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The marine environment has been represented as the most vital source of bioactive constituents. In the present work, we intended to purification, characterization, and investigation of the apoptotic effect of the bioactive molecule from edible mollusc Donax variabilis on NSCLC cell lines. The bioactive molecules were purified using Anion Exchange Chromatographic method with different millimolar of NaCl concentrations. The fractions were further performed to check the proliferative effect on A549 and NCI-H23 cells by MTT assay. The molecular mass of the active purified protein fraction (PPF-V) was 40 kDa, determined by SDS-PAGE. Furthermore, MALDI-TOF/MS was disclosed that it shared a 91% protein sequence similarity with FMRFamide peptide. Meanwhile, the apoptotic effect of PPF-V on A549 and NCI-H23 cells were investigated. Immunoblot analysis found that the PPF-V treatment groups showed the upregulation of Bax, Cytochrome-c, Cleaved Caspase-9 and 3 expression and downregulation of Bcl-2 protein expression on both NSCLC cell lines. In addition, purified protein fraction induced the loss of mitochondrial membrane potential and generation of reactive oxygen species (ROS) on lung cancer cells. Altogether, our results proved that the PPF-V from Donax variabilis inducing apoptosis against NSCLC cell lines, which could be a potential natural candidate for lung cancer therapy.

The marine invertebrates are one of the main leads to the derivation of anticancer compounds. The present investigation was to explore the anticancer effect of a potentially active polysaccharide fraction from marine clam Donax variabilis on human lung cancer cells (A549). A polysaccharide was purified from the whole tissue of D. variabilis by GFC and characterized by FT-IR and NMR spectroscopy. Subsequently, fraction 2.1 (F2.1) showed a significant inhibitory effect on A549 cells (p

The aim of this study is to investigate on anticancer properties of crude skin extract from marine catfish Tachysurus dussumieri on human colon cancer cell line. The biochemical contents protein, lipid and carbohydrate were estimated to be 1.02, 2.96 and 3.26 mg/ml respectively. The protein profiles were analysed by NATIVE and SDS-PAGE, results indicated both high and low molecular weight proteins ranging from 135 to 25 kDa. The crude extract was tested for its anticancer activity against human colon cancer cell line (HT 29) by MTT assay, the Inhibition concentration (IC50) was obtained on 600 μg/ml at 24 h. Fluorescent microscopy was used to study the cell morphology, nuclear condensation, early and late apoptotic stages by definite staining methods. DNA fragmentation and flow cytometric results revealed that the skin extract induces chromatin condensation, apoptotic cell death and also induce cell cycle arrest at G0/G1 phase on colon cancer cell line.