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402 results on '"Goto, Masatoshi"'

25. Indigenous opportunistic bacteria inhabit mammalian gut-associated lymphoid tissues and share a mucosal antibody-mediated symbiosis

Author

Obata, Takashi, Goto, Yoshiyuki, Kunisawa, Jun, Sato, Shintaro, Sakamoto, Mitsuo, Setoyama, Hiromi, Matsuki, Takahiro, Nonaka, Kazuhiko, Shibata, Naoko, Gohda, Masashi, Kagiyama, Yuki, Nochi, Tomonori, Yuki, Yoshikazu, Fukuyama, Yoshiko, Mukai, Akira, Shinzaki, Shinichiro, Fujihashi, Kohtaro, Sasakawa, Chihiro, Iijima, Hideki, Goto, Masatoshi, Umesaki, Yoshinori, Benno, Yoshimi, Kiyono, Hiroshi, and Curtiss, Roy

Published
2010

29. A New ICEclc Subfamily Integrative and Conjugative Element Responsible for Horizontal Transfer of Biphenyl and Salicylic Acid Catabolic Pathway in the PCB-Degrading Strain Pseudomonas stutzeri KF716

Author

Hirose, Jun, primary, Watanabe, Takahito, additional, Futagami, Taiki, additional, Fujihara, Hidehiko, additional, Kimura, Nobutada, additional, Suenaga, Hikaru, additional, Goto, Masatoshi, additional, Suyama, Akiko, additional, and Furukawa, Kensuke, additional

Published
2021
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43. Cross-regulation of biphenyl- and salicylate-catabolic genes by two regulatory systems in Pseudomonas pseudoalcaligenes KF707

Author

Fujihara, Hidehiko, Yoshida, Hideyuki, Matsunaga, Tetsuya, Goto, Masatoshi, and Furukawa, Kensuke

Subjects
Pseudomonas -- Physiological aspects, Bacterial genetics -- Research, Bacterial proteins -- Research, Biological sciences
Abstract

Pseudomonas pseudoalcaligenes KF707 grows on biphenyl and salicylate as sole sources of carbon. The biphenyl-catabolic (bph) genes are organized as bphR1A1A2(orf3)A3A4BCXOX1X2X3D, encoding the enzymes for conversion of biphenyl to acetyl coenzyme A. In this study, the salicylate-catabolic (sal) gene cluster encoding the enzymes for conversion of salicylate to acetyl coenzyme A were identified 6.6-kb downstream of the bph gene cluster along with a second regulatory gene, bphR2. Both the bph and sal genes were cross-regulated positively and/or negatively by the two regulatory proteins, BphR1 and BphR2, in the presence or absence of the effectors. The BphR2 binding sequence exhibits homology with the NahR binding sequences in various naphthalene-degrading bacteria. Based on previous studies and the present study we propose a new regulatory model for biphenyl and salicylate catabolism in strain KF707.

Published
2006

44. Protein O-mannosyltransferase A of Aspergillus awamori is involved in O-mannosylation of glucoamylase I

Author

Oka, Takuji, Sameshima, Yuka, Koga, Tomoko, Kim, Hoon, Goto, Masatoshi, and Furukawa, Kensuke

Subjects
Aspergillus -- Structure, Aspergillus -- Genetic aspects, Morphology -- Research, Genetic code -- Research, Biological sciences
Abstract

Industrially important extracellular enzymes from filamentous fungi are often O-mannosylated. The structure and function of the pmtA (AapmtA) gene encoding the protein O-D-mannosyltransferase of Aspergillus awamori were characterized. The AapmtA disruptant, designated AaPMTA, was constructed by homologous recombination. The strain AaPMTA exhibited fragile cell morphology with respect to hyphal extension, as well as swollen hyphae formation and conidia formation in potato dextrose medium. Moreover, the AapmtA disruptant showed increased sensitivity to high temperature and Congo red. Thus, the AaPmtA protein is involved in the formation of the normal cell wall. The strain AaPMTA could grow well in liquid synthetic medium and secrete glucoamylase I (GAl-AaPMTA) to a similar extent to the wild-type strain (GAl-WT). Matrix-assisted laser desorption ionization time-of-flight mass spectrometry analysis of the GAls revealed that approximately 33 mannose moieties of GAl were absent in strain AaPMTA. This result indicates that the AaPmtA protein is responsible for the transfer of mannose to GAl. Structural analysis of the O-linked oligosaccharides of GAl also demonstrated that the AapmtA disruption resulted in a reduction of the amounts of O-linked oligosaccharides, such as D-mannose and [alpha]-1,2-mannotriose, in GAl-AaPMTA. However, the amount of [alpha]-1,2-mannobiose was comparable between GAl-WT and GAl-AaPMTA. The result suggests the presence of a compensatory mechanism in the synthetic pathway of O-mannosylation in A. awamori.

Published
2005

45. Double relaxation oscillation SQUID with a 4JL on-chip digital flux locked-loop circuit

Author

Myoren, Hiroaki, Goto, Masatoshi, Taino, Tohru, Kikuchi, Katsuya, Nakagawa, Hiroshi, Tokoro, Kazuhiko, Aoyagi, Masahiro, and Takada, Susumu

Subjects
Relaxation phenomena -- Analysis, Integrated circuit fabrication -- Methods, Digital-to-analog converters -- Design and construction, Superconducting quantum interference devices -- Design and construction, Integrated circuit fabrication, Business, Electronics, Electronics and electrical industries
Abstract

A design of an up/down counter composed of 4-Junction Logic (JL) gates and the digital/analog (D/A) converter using an R-2R ladder circuit is presented to realize the digital flux locked-loop (FLL) circuit. The 4 JL gates with the Superconducting QUantum Interference Device (SQUID) regulator is developed and the correct logic operations are successfully confirmed using fabricated 4JL gates.

Published
2005

46. Efficient fabrication process for superconducting integrated circuits using photosensitive polyimide insulation layers

Author

Kikuchi, Katsuya, Goto, Masatoshi, Nakagawa, Hiroshi, Aoyagi, Masahiro, Segawa, Shigemasa, Takada, Susumu, Tokoro, Kazuhiko, Taino, Tohru, and Myoren, Hiroaki

Subjects
Polyimides -- Electric properties, Superconductive devices -- Design and construction, Integrated circuit fabrication -- Methods, Integrated circuit fabrication, Business, Electronics, Electronics and electrical industries
Abstract

Photosensitive polyimide insulation layers are introduced to fabricate superconducting integrated circuits. It simplifies the fabrication process, because the photosensitive polyimide insulation layer can be patterned by conventional photolithography process, resulting in the etching process unnecessary in the present new fabrication process.

Published
2005

47. Molecular characterization of protein O-mannosyltransferase and its involvement in cell-wall synthesis in Aspergillus nidulans

Author

Oka, Takuji, Hamaguchi, Tetsu, Sameshima, Yuka, Goto, Masatoshi, and Furukawa, Kensuke

Subjects
Bacterial cell walls -- Research, Glycosylation -- Research, Metabolic regulation -- Research, Aspergillus -- Research, Aspergillus -- Physiological aspects, Proteins, Biological sciences
Abstract

Protein O-glycosylation is essential for protein modification and plays important roles in eukaryotic cells. O-Mannosylation of proteins occurs in the filamentous fungus Aspergillus. The structure and function of the pmtA gene, encoding protein O-D-mannosyltransferase, which is responsible for the initial O-mannosylation reaction in Aspergillus nidulans, was characterized. Disruption of the pmtA gene resulted in the reduction of in vitro protein O-D-mannosyltransferase activity to 6% of that of the wild-type strain and led to underglycosylation of an extracellular glucoamylase. The pmtA disruptant exhibited abnormal cell morphology and alteration in carbohydrate composition, particularly reduction in the skeletal polysaccharides in the cell wall. The results indicate that PmtA is required for the formation of a normal cell wall in A. nidulans.

Published
2004

48. Engineering of polyploid Saccharomyces cerevisiae for secretion of large amounts of fungal glucoamylase

Author

Ekino, Keisuke, Hayashi, Hiroyuki, Moriyama, Masahiro, Matsuda, Minoru, Goto, Masatoshi, Yoshino, Sadazo, and Furukawa, Kensuke

Subjects
Brewer's yeast -- Genetic aspects, Brewer's yeast -- Physiological aspects, Polyploidy -- Research, Secretion -- Genetic aspects, Secretion -- Physiological aspects, Fungi -- Physiological aspects, Gene expression -- Physiological aspects, Biological sciences
Abstract

Research has been conducted on Saccharomyces cerevisiae polyploid cells. The expression of glucoamylase in S. cerevisiae cells has been carried out, and the results demonstrate that glucoamylase genes are integrated into delta-sequence.

Published
2002

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