1. Photodynamic Therapy Associated with a Blue Dye Papain-Based Gel and Evaluation of Its Degradation of Type I Collagen Fibers
- Author
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R. A. Mesquita-Ferrari, Sergio Brossi Botta, Patricia Aparecida Ana, Gonçalves Mll, Fernandes Kps, Brugnera A Junior, de Araújo Prates R, and Sandra Kalil Bussadori
- Subjects
medicine.medical_treatment ,Biomedical Engineering ,Photodynamic therapy ,In Vitro Techniques ,Collagen Type I ,030207 dermatology & venereal diseases ,03 medical and health sciences ,chemistry.chemical_compound ,Structure-Activity Relationship ,0302 clinical medicine ,medicine ,In vitro study ,Organic chemistry ,Animals ,Humans ,Radiology, Nuclear Medicine and imaging ,Analysis of Variance ,Blue dye ,Chromatography ,Photosensitizing Agents ,Chemistry ,030206 dentistry ,Methylene Blue ,Papain ,Photochemotherapy ,Case-Control Studies ,Degradation (geology) ,Cattle ,Gels ,After treatment ,Type I collagen - Abstract
The aim of this in vitro study was to evaluate the degradation of type I collagen fibers after treatment with a papain-based gel associated with a blue dye (PapaMBlue™) for use in antimicrobial photodynamic therapy.For such, 60 bioabsorbable membrane sponge discs were used. Group 1 was the negative control group. In groups 2, 3, and 4, the papain-based gel PapaMBlue gel was applied all over the samples for 4 min and irradiated using red laser (660 ± 10 nm) with 15, 30, and 40 J/cmNeither classic Papacarie™ nor the modified product with a photosensitizing agent (PapaMBlue) promoted collagen degradation.The irradiation of methylene blue added to papain gel with red light did not alter the chemical structure of type I collagen.
- Published
- 2017