32 results on '"Gola C"'
Search Results
2. Clinical significance and in vitro cellular regulation of hypoxia mimicry on HIF-1α and downstream genes in canine appendicular osteosarcoma
- Author
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Gola, C., Iussich, S., Noury, S., Martano, M., Gattino, F., Morello, E., Martignani, E., Maniscalco, L., Accornero, P., Buracco, P., Aresu, L., and De Maria, R.
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- 2020
- Full Text
- View/download PDF
3. Non-toxic acid-free glyoxal fixative for veterinary histopathology, immunohistochemistry and molecular analysis
- Author
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Zappulli, V., primary, Torrigiani, F., additional, Detillo, P., additional, Iussich, S., additional, Rondena, M., additional, Gola, C., additional, MInoli, L., additional, Morello, E., additional, Ferraris, E.I., additional, Rigillo, A., additional, and Bussolati, B., additional
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- 2023
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4. Evaluating diffusion model generated synthetic histopathology image data against authentic digital pathology images
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Tomaszewski, John E., Ward, Aaron D., Rai, T., Gola, C., Hernández, M., Fingerhood, S., Marrero, J., Diaz-Santana, P., Giglia, G., Morisi, A., Bacci, B., Thomas, S. A., Ressel, L., Bacon, N., Papachristou, N., Cook, A., La Ragione, R., and Wells, K.
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- 2024
- Full Text
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5. Genomic and Transcriptomic Characterization of Canine Osteosarcoma Cell Lines: A Valuable Resource for Translational Medicine
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Gola, C., primary, Giannuzzi, D., additional, Rinaldi, A., additional, Iussich, S., additional, Modesto, P., additional, Morello, E., additional, Buracco, P., additional, Aresu, L., additional, and De Maria, R., additional
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- 2022
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6. Amoxicillin administration to a patient with infectious mononucleosis: Report of an adverse reaction case
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García,MC, Cavigliasso,J, Gola,C, Valentín,R, Seguro,ML, and Romañuk,CB
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error de medicación ,purl.org/becyt/ford/1 [https] ,mononucleosis infecciosa ,ADVERSE REACTION ,INFECTIOUS MONONUCLEOSIS ,purl.org/becyt/ford/1.4 [https] ,reacción adversa ,purl.org/becyt/ford/3 [https] ,purl.org/becyt/ford/3.1 [https] ,MEDICATION ERROR ,AMOXICILLIN ,Amoxicilina - Abstract
Describir un caso clínico de un paciente tratado con amoxicilina (AMX) mientras cursaba una infección viral, en el que se detectaron errores de medicación (EM) y reacciones adversas a medicamentos (RAM). Descripción del caso clínico: Paciente masculino de 7 años y 23 kg concurrió a la guardia del hospital presentando fatiga, fiebre y ganglios linfáticos inflamados. Se diagnosticó faringitis bacteriana y se indicó AMX 50 mg/kg/día vía oral/8 h. Al día siguiente, el paciente fue nuevamente al hospital presentando rash cutáneo en todo el cuerpo. Se advirtió evento adverso (EA) por diagnóstico erróneo y se diagnosticó mononucleosis infecciosa (MI). Se suspendió la AMX y hubo remisión del rash. Un farmacéutico hospitalario realizó la imputación utilizando el algoritmo de Naranjo (puntaje 5-8) y notificó al Sistema Unificado de Farmacovigilancia de Córdoba. Discusión: Es fundamental el diagnóstico adecuado de la MI para evitar el uso inapropiado de antibióticos ante una infección viral. En el caso descripto ocurrió un EM en la etapa de prescripción, debido al diagnóstico incorrecto, y una RAM por el uso de AMX. El puntaje obtenido permitió catalogar a esta RAM como probable, no pudiendo ser considerada definida/probada por no haber reexposición; aunque el EA apareció luego de la administración de un fármaco sospechoso y no existieron causas alternativas para explicar esta reacción. Además, el EA desapareció tras suspender la AMX. Esto enfatiza el rol protagónico del farmacéutico para promover la seguridad de pacientes y la importancia de las notificaciones. Goal: To describe a clinical case of a patient treated with amoxicillin (AMX) while he had a viral infection. In this case, medication error (ME) as well as adverse drug reaction (ADR) were detected. Description of the clinical case: A 7- year-old, 23-kg male patient attended to the hospital with the following symptoms: fatigue, fever and swollen lymph nodes. Pharyngitis caused by bacteria was diagnosed and orally administration of AMX 50 mg/kg/day each 8 h was indicated. One day later, the patient returned to the hospital with skin rash throughout the body. An adverse event (AE) was noticed due to an error in the diagnosis, which was corrected and infectious mononucleosis (MI) was detected. AMX was suspended and remission of the rash was observed. A hospital pharmacist performed the imputation using the Naranjo algorithm (score 5-8) and notified to the Sistema Unificado de Farmacovigilancia of Cordoba. Discussion: Proper diagnosis of IM is essential to avoid inappropriate use of antibiotics when the patients present viral infections. In this clinical case, a ME occurred at the prescription, due to the incorrect diagnosis, and an ADR because of the use of AMX. The score obtained allowed us to classify this ADR as probable, and it could not be considered definite/proven because there was no re-exposure. However, the AE occurred after the administration of a suspected drug and there were no alternative causes to explain this reaction. In addition, the AE disappeared after suspending the AMX. This emphasizes the leading role of the pharmacist in promoting patient safety and the relevance of notifications. Fil: García, Mónica Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina Fil: Cavigliasso, Julieta. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina Fil: Gola, Camila. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina Fil: Valentín, Rocío. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina Fil: Seguro, María Laura. No especifíca; Fil: Romañuk, Carolina Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
- Published
- 2020
7. An Histological and Immunohistochemical Assessment of Canine Free Movable Thyroid Carcinomas
- Author
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Gola, C., primary, De Maria, R., additional, Morello, E., additional, Iussich, S., additional, and Guscetti, F., additional
- Published
- 2018
- Full Text
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8. ATTIVITÀ DELLA S.O.I. L'ITALIA ALLE « FLORALIES » DI GAND
- Author
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Gola, C. and Morettini, A.
- Published
- 1960
9. Séparation et identification par chromatographie sur couche mince de cinq antidépresseurs thymoanaleptiques dérivés de la dibenzoazépine
- Author
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Viala, A., primary, Gouezo, F., additional, and Gola, C., additional
- Published
- 1969
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10. Identification des antidépresseurs tricycliques dérivés de l'imipramine par chromatographie en phase gazeuse
- Author
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Viala, A., primary, Cano, J.P., additional, Gola, C., additional, and Gouezo, F., additional
- Published
- 1971
- Full Text
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11. Evaluation of the neoplastic infiltration of the skin overlying canine subcutaneous soft tissue sarcomas: An explorative study
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Marina Martano, Damiano Stefanello, Federico Massari, Davide Giacobino, Lisa Adele Piras, Boris Dalpozzo, Selina Iussich, Cecilia Gola, Paolo Buracco, Maurizio Annoni, Sara Del Magno, Emanuela Maria Morello, and Del Magno S, Morello E, Iussich S, Gola C, Dalpozzo B, Annoni M, Martano M, Massari F, Giacobino D, Piras LA, Stefanello D, Buracco P.
- Subjects
Wide excision ,Pathology ,medicine.medical_specialty ,Skin Neoplasms ,040301 veterinary sciences ,Soft Tissue Neoplasms ,0403 veterinary science ,histology ,03 medical and health sciences ,Dogs ,0302 clinical medicine ,Dermis ,histologic margin ,histologic margin, histology, skin infiltration, soft tissue sarcoma, subcutaneous sarcoma, tumor recurrence ,medicine ,Animals ,Dog Diseases ,tumor recurrence ,Curative intent ,General Veterinary ,business.industry ,Soft tissue sarcoma ,Soft tissue ,Sarcoma ,Histology ,04 agricultural and veterinary sciences ,medicine.disease ,medicine.anatomical_structure ,skin infiltration ,030220 oncology & carcinogenesis ,soft tissue sarcoma ,Cutaneous tumor ,Neoplasm Recurrence, Local ,subcutaneous sarcoma ,business ,Infiltration (medical) - Abstract
Studies regarding the neoplastic infiltration of the skin overlying canine subcutaneous soft tissue sarcoma (sSTS) are lacking. In case of the absence of tumor infiltration, there would be the possibility of leaving this unaffected skin in place, thus simplifying surgery. The aim of the study was to investigate whether the skin overlying sSTSs is infiltrated by neoplastic cells. Dogs with sSTSs treated surgically were prospectively enrolled. After excision, the skin was dissected from the tumor along the natural surgical plane of cleavage and histologically evaluated. Twenty-nine dogs with an sSTS were included (22 grade I, 6 grade II, and 1 grade III). The sSTS-overlying skin was not tumor-infiltrated in 14/29 cases (48.3%). A higher frequency of infiltration was observed in higher grade sSTSs (grades II and III, 100%; P = .006); nevertheless, 8/22 grade I sSTSs (36%) also showed cutaneous infiltration. This infiltration involved the dermis of the skin directly in contact with the tumor (multifocal in 11 and diffuse in four cases). Although the cutaneous tumor infiltration is less frequent in grade I sSTSs and a wide excision may still be the safest treatment for any sSTS for a greater possibility of local control, this study opens the possibility to a less aggressive cutaneous excision, but still with a local curative intent, as only the skin directly in contact with the sSTS has been proven to be tumor-infiltrated. Additional studies are warranted to confirm that excision of only this skin may guarantee a complete local control, especially in lower-grade sSTSs.
- Published
- 2021
12. Doxorubicin treatment modulates chemoresistance and affects the cell cycle in two canine mammary tumour cell lines
- Author
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Barbara Brunetti, Cecilia Gola, Antonio Brocco, Monica Forni, Cinzia Benazzi, Asia Marangio, Chiara Bernardini, Raffaella De Maria, Augusta Zannoni, Roberta Salaroli, Giuseppe Sarli, Luisa Vera Muscatello, Federico Parenti, Michela Levi, Levi M., Salaroli R., Parenti F., De Maria R., Zannoni A., Bernardini C., Gola C., Brocco A., Marangio A., Benazzi C., Muscatello L.V., Brunetti B., Forni M., and Sarli G.
- Subjects
p53 ,Proliferation index ,Population ,Canine mammary tumour ,Mammary Neoplasms, Animal ,P-glycoprotein ,03 medical and health sciences ,0302 clinical medicine ,Dogs ,Cell Line, Tumor ,ATP Binding Cassette Transporter, Subfamily G, Member 2 ,Animals ,Telomerase reverse transcriptase ,ATP Binding Cassette Transporter, Subfamily B, Member 1 ,education ,BCRP ,Cell cycle ,Cell line ,Chemoresistance ,Doxorubicin ,Telomerases ,030304 developmental biology ,Cell Proliferation ,0303 health sciences ,education.field_of_study ,lcsh:Veterinary medicine ,General Veterinary ,biology ,Cell growth ,Chemistry ,Cell Cycle ,General Medicine ,Neoplasm Proteins ,Gene Expression Regulation, Neoplastic ,Apoptosis ,Drug Resistance, Neoplasm ,030220 oncology & carcinogenesis ,Cancer research ,biology.protein ,Neoplastic cell ,lcsh:SF600-1100 ,Multidrug Resistance-Associated Proteins ,Research Article - Abstract
Background Doxorubicin (DOX) is widely used in both human and veterinary oncology although the onset of multidrug resistance (MDR) in neoplastic cells often leads to chemotherapy failure. Better understanding of the cellular mechanisms that circumvent chemotherapy efficacy is paramount. The aim of this study was to investigate the response of two canine mammary tumour cell lines, CIPp from a primary tumour and CIPm, from its lymph node metastasis, to exposure to EC50(20h) DOX at 12, 24 and 48 h of treatment. We assessed the uptake and subcellular distribution of DOX, the expression and function of P-glycoprotein (P-gp) and Breast Cancer Resistance Protein (BCRP), two important MDR mediators. To better understand this phenomenon the effects of DOX on the cell cycle and Ki67 cell proliferation index and the expression of p53 and telomerase reverse transcriptase (TERT) were also evaluated by immunocytochemistry (ICC). Results Both cell lines were able to uptake DOX within the nucleus at 3 h treatment while at 48 h DOX was absent from the intracellular compartment (assessed by fluorescence microscope) in all the surviving cells. CIPm, originated from the metastatic tumour, were more efficient in extruding P-gp substrates. By ICC and qRT-PCR an overall increase in both P-gp and BCRP were observed at 48 h of EC50(20h) DOX treatment in both cell lines and were associated with a striking increase in the percentage of p53 and TERT expressing cells by ICC. The cell proliferation fraction was decreased at 48 h in both cell lines and cell cycle analysis showed a DOX-induced arrest in the S phase for CIPp, while CIPm had an increase in cellular death without arrest. Both cells lines were therefore composed by a fraction of cells sensible to DOX that underwent apoptosis/necrosis. Conclusions DOX administration results in interlinked modifications in the cellular population including a substantial effect on the cell cycle, in particular arrest in the S phase for CIPp and the selection of a subpopulation of neoplastic cells bearing MDR phenotype characterized by P-gp and BCRP expression, TERT activation, p53 accumulation and decrease in the proliferating fraction. Important information is given for understanding the dynamic and mechanisms of the onset of drug resistance in a neoplastic cell population.
- Published
- 2020
13. La disciplina della perequazione urbanistica nella ricostruzione del dato giurisprudenziale
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Martini, P. Stella Richter, P. Urbani, M. Gola, C. Iaione, A. Giusti, G. Iudica, C. Cacciavillani, G. Martini, M.T. Paola Caputi Jambrenghi, A. Angiuli, L. Coen, G. Iacovone, M. di Donna, T. Bonetti, F. Ancora, P. Otranto, C. Lamberti, G. Mastrodonato, P. Mantini, M.V. Lumetti, G. Guzzardo, P. Stella Richter, and Martini, Giovanni
- Published
- 2018
14. D-galactonate metabolism in enteric bacteria: a molecular and physiological perspective.
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Singh S, Gola C, Singh B, Agrawal V, and Chaba R
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- Gene Expression Regulation, Bacterial, Animals, Humans, Operon, Enterobacteriaceae metabolism, Enterobacteriaceae genetics, Sugar Acids metabolism
- Abstract
D-galactonate, a widely prevalent sugar acid, was first reported as a nutrient source for enteric bacteria in the 1970s. Since then, decades of research enabled a description of the modified Entner-Doudoroff pathway involved in its degradation and reported the structural and biochemical features of its metabolic enzymes, primarily in Escherichia coli K-12. However, only in the last few years, the D-galactonate transporter has been characterized, and the regulation of the dgo operon, encoding the structural genes for the transporter and enzymes of D-galactonate metabolism, has been detailed. Notably, in recent years, multiple evolutionary studies have identified the dgo operon as a dominant target for adaptation of E. coli in the mammalian gut. Despite considerable research on dgo operon, numerous fundamental questions remain to be addressed. The emerging relevance of the dgo operon in host-bacterial interactions further necessitates the study of D-galactonate metabolism in other enterobacterial strains., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
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15. Cryptosporidium andersoni- associated proliferative abomasitis in a roan antelope.
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Fingerhood S, Shotton J, Gola C, and Betson M
- Abstract
A 2-y-old, intact male roan antelope ( Hippotragus equinus ) was submitted for routine postmortem investigation after a prolonged history of diarrhea and weight loss. The abomasal mucosa was diffusely thickened and corrugated. Abomasal gland hyperplasia was associated with abundant apical organisms consistent with Cryptosporidium spp. Genomic DNA was extracted from abomasal and intestinal contents and subjected to PCR using primers specific for the 18S rRNA gene of Cryptosporidium spp., followed by Sanger sequencing. The sequence was >99% homologous to Cryptosporidium andersoni. C. andersoni -associated proliferative abomasitis has not been reported previously in a captive hippotraginid, to our knowledge., Competing Interests: Declaration of conflicting interestsThe authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
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- 2024
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16. Hypoxia-associated markers in the prognosis of oral canine melanoma.
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Gola C, Maniscalco L, Iussich S, Morello E, Olimpo M, Martignani E, Accornero P, Giacobino D, Mazzone E, Modesto P, Varello K, Aresu L, and De Maria R
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- Dogs, Animals, Prognosis, Male, Female, Vascular Endothelial Growth Factor A metabolism, Vascular Endothelial Growth Factor A genetics, Receptors, CXCR4 metabolism, Receptors, CXCR4 genetics, Cell Line, Tumor, Hypoxia veterinary, Immunohistochemistry veterinary, Carbonic Anhydrase IX metabolism, Carbonic Anhydrase IX genetics, Tumor Microenvironment, Mouth Neoplasms veterinary, Mouth Neoplasms pathology, Mouth Neoplasms metabolism, Mouth Neoplasms diagnosis, Dog Diseases pathology, Dog Diseases metabolism, Melanoma veterinary, Melanoma pathology, Melanoma metabolism, Melanoma diagnosis, Biomarkers, Tumor metabolism, Hypoxia-Inducible Factor 1, alpha Subunit metabolism
- Abstract
Canine oral malignant melanoma (COMM) is the most common neoplasm in the oral cavity characterized by local invasiveness and high metastatic potential. Hypoxia represents a crucial feature of the solid tumor microenvironment promoting cancer progression and drug resistance. Hypoxia-inducible factor-1α (HIF-1α) and its downstream effectors, vascular endothelial growth factor A (VEGF-A), glucose transporter isoform 1 (GLUT1), C-X-C chemokine receptor type 4 (CXCR4), and carbonic anhydrase IX (CAIX), are the main regulators of the adaptive response to low oxygen availability. The prognostic value of these markers was evaluated in 36 COMMs using immunohistochemistry. In addition, the effects of cobalt chloride-mediated hypoxia were evaluated in 1 primary COMM cell line. HIF-1α expression was observed in the nucleus, and this localization correlated with the presence or enhanced expression of HIF-1α-regulated genes at the protein level. Multivariate analysis revealed that in dogs given chondroitin sulfate proteoglycan-4 ( CSPG4 ) DNA vaccine, COMMs expressing HIF-1α, VEGF-A, and CXCR4 were associated with shorter disease-free intervals (DFI) compared with tumors that were negative for these markers ( P = .03), suggesting hypoxia can influence immunotherapy response. Western blotting showed that, under chemically induced hypoxia, COMM cells accumulate HIF-1α and smaller amounts of CAIX. HIF-1α induction and stabilization triggered by hypoxia was corroborated by immunofluorescence, showing its nuclear translocation. These findings reinforce the role of an hypoxic microenvironment in tumor progression and patient outcome in COMM, as previously established in several human and canine cancers. In addition, hypoxic markers may represent promising prognostic markers, highlighting opportunities for their use in therapeutic strategies for COMMs., Competing Interests: Declaration of Conflicting InterestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
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- 2024
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17. Meningoencephalomyelitis and brachial plexitis in a dog infected with louping ill virus.
- Author
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Fingerhood S, Mansfield KL, Folly AJ, Gomez Vitores A, Rocchi M, Clarke D, and Gola C
- Abstract
A foxhound from a hunting kennel in the United Kingdom was euthanized after being hospitalized with progressive neurologic signs, including tremors, seizures, and obtunded mentation. No abnormalities were appreciated on gross postmortem examination. Histologically, severe meningoencephalomyelitis and mild neuritis of the brachial plexus were present. Molecular analysis of brain tissue detected louping ill virus. In addition, louping ill virus-specific antigens were detected in neurons within the brainstem, the entire length of the spinal cord, as well as in rare cells in the brachial plexus using immunohistochemistry. The genetic sequence of the virus appears most closely related to a previously detected virus in a dog from a similar geographic location in 2015. This is the first characterization of the inflammatory lesions and viral distribution of louping ill virus in a naturally infected dog within the spinal cord and brachial plexus., Competing Interests: Declaration of Conflicting InterestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
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- 2024
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18. Presepsin Levels in Pediatric Patients with Fever and Suspected Sepsis: A Pilot Study in an Emergency Department.
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Gatto A, Mantani L, Gola C, Pansini V, Di Sarno L, Capossela L, Ferretti S, Graglia B, and Chiaretti A
- Abstract
Sepsis is a life-threatening condition that affects 1.2 million children annually. Although there are several criteria for diagnosing this condition, signs are often nonspecific, and identifying sepsis is challenging. In this context, presepsin (P-SEP) seems to be a promising new biomarker since its plasma levels increase earlier than other sepsis-related proteins and its measurement is faster. We enrolled 157 minors who presented to the Pediatric Emergency Department of Agostino Gemelli Hospital with fever and suspected sepsis. Biochemical, anamnestic, and clinical data were collected. Viral agents were identified as the causative factor in 64 patients, who had an average P-SEP value of 309.04 pg/mL (SD ± 273.2), versus an average P-SEP value of 526.09 pg/mL (SD ± 657) found in 27 bacterial cases ( p value: 0.0398). Four cases of overt sepsis had an average P-SEP value of 3328.5 pg/mL (SD ± 1586.6). The difference in P-SEP levels in viral versus bacterial infections was found to be statistically significant; therefore, P-SEP may have a central role in the evaluation of febrile children, helping clinicians distinguish between these two etiologies. Furthermore, amongst the cases of confirmed sepsis, P-SEP was always greater than 2000 pg/mL, while C-reactive protein and procalcitonin values appeared lower than what was considered significant.
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- 2024
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19. Dissecting aortitis in a goat associated with Pasteurella multocida and Staphylococcus spp infection.
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Gola C, Fingerhood S, Parry NM, and Diaz-Delgado J
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- Animals, Female, Pasteurella multocida, Goats, Goat Diseases microbiology, Goat Diseases pathology, Pasteurella Infections veterinary, Staphylococcal Infections veterinary, Aortic Dissection veterinary
- Abstract
Reports of primary cardiovascular disease in goats are rare and most commonly include ventricular septal defect, valvular endocarditis, traumatic pericarditis, ionophore poisoning and nutritional cardiomyopathies. We now report the pathological findings in a 67 kg, 6-year-old, adult female Boer goat that presented with neurological signs (ie, head pressing, unsteadiness and paddling) and hyperthermia 2 days prior to death. Lack of therapeutic response to meloxicam and penicillin‒streptomycin and poor prognosis led to euthanasia of the animal. At necropsy, the main findings included severe aortic dissection with luminal thrombosis and stenosis, and pulmonary congestion and oedema. Histological examination of the aorta revealed severe chronic granulomatous and fibrosing dissecting aortitis with mineralization. Bacterial culture of the affected aortic segment resulted in isolation of a profuse growth of Pasteurella multocida and a moderate growth of Staphylococcus spp. Histopathological findings in the central nervous system were consistent with neurolisteriosis., (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
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- 2024
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20. Fatal cerebrovascular accident in a captive red panda (Ailurus fulgens fulgens) with concurrent amdoparvovirus infection.
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Gola C, Kvapil P, Kuhar U, Diaz-Delgado J, Alex CE, Shotton J, Smith SJ, and Fingerhood S
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- Male, Animals, Kidney, Hemorrhage veterinary, Ailuridae, Stroke veterinary
- Abstract
We report the pathological and molecular findings in an adult male Himalayan red panda (Ailurus fulgens fulgens) whose death was attributed to parenchymal brain haemorrhage (PBH) of the thalamus. Post-mortem examination revealed severe, acute PBH and intraventricular haemorrhage with major involvement of the thalamus, as well as scattered chronic microinfarctions. Vascular disease in the brain and other organs was suggestive of systemic hypertension. Histological lesions included arteriolar hyalinosis and varying degrees of arteriosclerosis, arterial tunica media hypertrophy and hyperplasia and infiltration of arterial walls by lipid-laden macrophages. Other relevant findings included marked myocardial fibrosis, lymphoplasmacytic tubulointerstitial nephritis, lymphoplasmacytic meningoencephalitis and chronic mitral valve degeneration. The changes in the cerebral vasculature were consistent with hypertensive encephalopathy and a cerebrovascular accident, specifically PBH, which has not been previously reported in this species. Additionally, polymerase chain reaction analysis for red panda amdoparvovirus (RPAV) was positive in the brain and kidneys. Preceded by hypertensive vascular changes and brain microinfarctions, sudden death in this animal likely resulted from fatal PBH with intraventricular haemorrhage. The clinicopathological role of RPAV infection is unknown in this case, although its contribution to the chronic renal disease is considered possible in the context of our current understanding of RPAV-associated pathology., (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2023
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21. Investigating a Prognostic Factor for Canine Hepatocellular Carcinoma: Analysis of Different Histological Grading Systems and the Role of PIVKA-II.
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Maniscalco L, Varello K, Morello E, Montemurro V, Olimpo M, Giacobino D, Abbamonte G, Gola C, Iussich S, and Bozzetta E
- Abstract
Background: Hepatocellular carcinoma (HCC) in dogs is uncommon and often associated with a good prognosis, although some cases prove to be aggressive. In human oncology HCC is often very aggressive and diagnostic methods and prognostic factors are widely used to predict its biological behaviour. These include the expression of PIVKA-II., Methods: in order to identify a prognostic factor for canine HCC, we applied different methods of histological grading and investigated PIVKA-II expression in 22 HCC of dogs treated surgically and followed clinically for at least 2 years., Results: Nineteen patients analysed have passed the observation period without tumour recurrence, while 3 died following the development of metastases. PIVKA-II was positive in 15/22 cases without correlation with prognosis or tumoural grading even if a trend of PIVKA-II negativity in low WHO grades as well as increased number of PIVKA-II positive cases in higher WHO grades weres observed., Conclusions: This work showed that, PIVKA-II cannot be considered either as a marker of malignancy or as a prognostic marker for canine HCC. The poor prognosis depends usually on the clinical presentation. Thus prognostic parameters in canine HCC able to predict its aggressive behaviour through histological examination are still missing. The most promising method, limited to our study, seems to be the WHO histological grading.
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- 2022
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22. The mitotic regulator polo-like kinase 1 as a potential therapeutic target for c-Myc-overexpressing canine osteosarcomas.
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Gola C, Licenziato L, Accornero P, Iussich S, Morello E, Buracco P, Modesto P, Aresu L, and De Maria R
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- Dogs, Animals, Humans, Cell Line, Tumor, Cell Cycle Proteins genetics, Cell Cycle Proteins metabolism, Cell Proliferation, Apoptosis, Polo-Like Kinase 1, Dog Diseases drug therapy, Osteosarcoma genetics, Osteosarcoma veterinary, Osteosarcoma drug therapy, Bone Neoplasms genetics, Bone Neoplasms veterinary, Bone Neoplasms drug therapy
- Abstract
Osteosarcoma is the most common primary malignant bone tumour in dogs, characterized by a locally aggressive and highly metastatic behaviour. Despite the current standards of care, most dogs succumb to the disease, indicating the need for novel treatment strategies. Polo-like kinase 1 (PLK1) is dysregulated in a variety of human cancer types, including osteosarcoma, and induces c-Myc accumulation. The crosstalk between the two molecules coordinates cell proliferation, differentiation, self-renewal and apoptosis. Therefore, PLK1 has recently emerged as a potential therapeutic target, mainly in tumours overexpressing c-Myc. BI 2536 is a selective PLK1 inhibitor promoting mitotic arrest and apoptosis in a variety of cancer cells. This research aimed at evaluating PLK1 and c-Myc protein expression in 53 appendicular canine osteosarcoma (cOSA) samples and the in vitro effects of BI 2536 on a c-Myc and PLK1-overexpressing cOSA cell line (D17). PLK1 and c-Myc expression in cOSA samples showed no correlation with clinicopathological data. However, c-Myc overexpression was associated with a significantly reduced overall survival (p = .003). Western Blot and RT-qPCR assays revealed that D17 expressed high protein and transcript levels of both PLK1 and MYC. When treated with BI 2536 (range 2.5-15 nM) for 24 h, D17 showed a substantial decrease in cell growth, inducing apoptosis and G
2 /M cell cycle arrest. Interestingly, under BI 2536 treatment, D17 showed decreased c-Myc protein levels. Consistent with human OSA, these preliminary data outline the prognostic value of c-Myc expression in cOSA and highlight the potential role of PLK1 as an antiproliferative therapeutic target for tumours overexpressing c-Myc., (© 2022 The Authors. Veterinary and Comparative Oncology published by John Wiley & Sons Ltd.)- Published
- 2022
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23. Evaluation of the neoplastic infiltration of the skin overlying canine subcutaneous soft tissue sarcomas: An explorative study.
- Author
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Del Magno S, Morello E, Iussich S, Gola C, Dalpozzo B, Annoni M, Martano M, Massari F, Giacobino D, Piras LA, Stefanello D, and Buracco P
- Subjects
- Animals, Dogs, Neoplasm Recurrence, Local veterinary, Dog Diseases surgery, Sarcoma veterinary, Skin Neoplasms veterinary, Soft Tissue Neoplasms surgery, Soft Tissue Neoplasms veterinary
- Abstract
Studies regarding the neoplastic infiltration of the skin overlying canine subcutaneous soft tissue sarcoma (sSTS) are lacking. In case of the absence of tumor infiltration, there would be the possibility of leaving this unaffected skin in place, thus simplifying surgery. The aim of the study was to investigate whether the skin overlying sSTSs is infiltrated by neoplastic cells. Dogs with sSTSs treated surgically were prospectively enrolled. After excision, the skin was dissected from the tumor along the natural surgical plane of cleavage and histologically evaluated. Twenty-nine dogs with an sSTS were included (22 grade I, 6 grade II, and 1 grade III). The sSTS-overlying skin was not tumor-infiltrated in 14/29 cases (48.3%). A higher frequency of infiltration was observed in higher grade sSTSs (grades II and III, 100%; P = .006); nevertheless, 8/22 grade I sSTSs (36%) also showed cutaneous infiltration. This infiltration involved the dermis of the skin directly in contact with the tumor (multifocal in 11 and diffuse in four cases). Although the cutaneous tumor infiltration is less frequent in grade I sSTSs and a wide excision may still be the safest treatment for any sSTS for a greater possibility of local control, this study opens the possibility to a less aggressive cutaneous excision, but still with a local curative intent, as only the skin directly in contact with the sSTS has been proven to be tumor-infiltrated. Additional studies are warranted to confirm that excision of only this skin may guarantee a complete local control, especially in lower-grade sSTSs., (© 2021 John Wiley & Sons Ltd.)
- Published
- 2021
- Full Text
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24. Genomic and Transcriptomic Characterization of Canine Osteosarcoma Cell Lines: A Valuable Resource in Translational Medicine.
- Author
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Gola C, Giannuzzi D, Rinaldi A, Iussich S, Modesto P, Morello E, Buracco P, Aresu L, and De Maria R
- Abstract
Osteosarcoma (OSA) represents the most common primary bone tumor in dogs and is characterized by a highly aggressive behavior. Cell lines represent one of the most suitable and reproducible pre-clinical models, and therefore the knowledge of their molecular landscape is mandatory to investigate oncogenic mechanisms and drug response. The present study aims at determining variants, putative driver genes, and gene expression aberrations by integrating whole-exome and RNA sequencing. For this purpose, eight canine OSA cell lines and one matched pair of primary tumor and normal tissue were analyzed. Overall, cell lines revealed a mean tumor mutational burden of 9.6 mutations/Mb (range 3.9-16.8). Several known oncogenes and tumor suppressor genes, such as ALK, MYC , and MET , were prioritized as having a likely role in canine OSA. Mutations in eight genes, previously described as human OSA drivers and including TP53, PTCH1, MED12 , and PI3KCA , were retrieved in our cell lines. When variants were cross-referenced with human OSA driver mutations, the E273K mutation of TP53 was identified in the Wall cell line and tumor sample. The transcriptome profiling detected two possible p53 inactivation mechanisms in the Wall cell line on the one hand, and in D17 and D22 on the other. Moreover, MET overexpression, potentially leading to MAPK/ERK pathway activation, was observed in D17 and D22 cell lines. In conclusion, our data provide the molecular characterization of a large number of canine OSA cell lines, allowing future investigations on potential therapeutic targets and associated biomarkers. Notably, the Wall cell line represents a valuable model to empower prospective in vitro studies both in human and in dogs, since the TP53 driver mutation was maintained during cell line establishment and was widely reported as a mutation hotspot in several human cancers., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Gola, Giannuzzi, Rinaldi, Iussich, Modesto, Morello, Buracco, Aresu and De Maria.)
- Published
- 2021
- Full Text
- View/download PDF
25. Doxorubicin treatment modulates chemoresistance and affects the cell cycle in two canine mammary tumour cell lines.
- Author
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Levi M, Salaroli R, Parenti F, De Maria R, Zannoni A, Bernardini C, Gola C, Brocco A, Marangio A, Benazzi C, Muscatello LV, Brunetti B, Forni M, and Sarli G
- Subjects
- ATP Binding Cassette Transporter, Subfamily B, Member 1 genetics, ATP Binding Cassette Transporter, Subfamily B, Member 1 metabolism, ATP Binding Cassette Transporter, Subfamily G, Member 2 genetics, ATP Binding Cassette Transporter, Subfamily G, Member 2 metabolism, Animals, Cell Line, Tumor, Cell Proliferation drug effects, Dogs, Gene Expression Regulation, Neoplastic drug effects, Mammary Neoplasms, Animal, Multidrug Resistance-Associated Proteins genetics, Multidrug Resistance-Associated Proteins metabolism, Neoplasm Proteins genetics, Neoplasm Proteins metabolism, Cell Cycle drug effects, Doxorubicin pharmacology, Drug Resistance, Neoplasm drug effects
- Abstract
Background: Doxorubicin (DOX) is widely used in both human and veterinary oncology although the onset of multidrug resistance (MDR) in neoplastic cells often leads to chemotherapy failure. Better understanding of the cellular mechanisms that circumvent chemotherapy efficacy is paramount. The aim of this study was to investigate the response of two canine mammary tumour cell lines, CIPp from a primary tumour and CIPm, from its lymph node metastasis, to exposure to EC50
(20h) DOX at 12, 24 and 48 h of treatment. We assessed the uptake and subcellular distribution of DOX, the expression and function of P-glycoprotein (P-gp) and Breast Cancer Resistance Protein (BCRP), two important MDR mediators. To better understand this phenomenon the effects of DOX on the cell cycle and Ki67 cell proliferation index and the expression of p53 and telomerase reverse transcriptase (TERT) were also evaluated by immunocytochemistry (ICC)., Results: Both cell lines were able to uptake DOX within the nucleus at 3 h treatment while at 48 h DOX was absent from the intracellular compartment (assessed by fluorescence microscope) in all the surviving cells. CIPm, originated from the metastatic tumour, were more efficient in extruding P-gp substrates. By ICC and qRT-PCR an overall increase in both P-gp and BCRP were observed at 48 h of EC50(20h) DOX treatment in both cell lines and were associated with a striking increase in the percentage of p53 and TERT expressing cells by ICC. The cell proliferation fraction was decreased at 48 h in both cell lines and cell cycle analysis showed a DOX-induced arrest in the S phase for CIPp, while CIPm had an increase in cellular death without arrest. Both cells lines were therefore composed by a fraction of cells sensible to DOX that underwent apoptosis/necrosis., Conclusions: DOX administration results in interlinked modifications in the cellular population including a substantial effect on the cell cycle, in particular arrest in the S phase for CIPp and the selection of a subpopulation of neoplastic cells bearing MDR phenotype characterized by P-gp and BCRP expression, TERT activation, p53 accumulation and decrease in the proliferating fraction. Important information is given for understanding the dynamic and mechanisms of the onset of drug resistance in a neoplastic cell population.- Published
- 2021
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26. CD3-CD20-positive nodal lymphoma with cross-lineage rearrangement in a dog.
- Author
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Nicoletti A, Aresu L, Marino M, Massaro M, Martignani E, Caporali E, Capuccini S, Bonfanti U, and Gola C
- Subjects
- Animals, Antigens, CD20 metabolism, CD3 Complex metabolism, Dog Diseases physiopathology, Dogs, Fluorescent Antibody Technique veterinary, Immunohistochemistry, Lymph Nodes pathology, Lymphoma, Large B-Cell, Diffuse diagnosis, Lymphoma, Large B-Cell, Diffuse genetics, Lymphoma, Large B-Cell, Diffuse physiopathology, Male, Antigens, CD20 genetics, CD3 Complex genetics, Dog Diseases diagnosis, Dog Diseases genetics, Gene Rearrangement, Lymphoma, Large B-Cell, Diffuse veterinary
- Abstract
A 7-y-old mixed-breed male dog was presented with a history of generalized lymphadenopathy. Fine-needle aspirates of the enlarged peripheral lymph nodes were suggestive of lymphoma. Histologic examination of a retromandibular lymph node was suggestive of high-grade, medium large-cell lymphoma. Immunohistochemistry revealed concurrent expression of CD3 and CD20. The co-localization of the 2 antigens was confirmed by immunofluorescence. PCR for antigen receptor gene rearrangements (PARR) detected clonal rearrangements for both T-cell receptor gamma and B-cell receptor. The final diagnosis was CD3-CD20-positive anaplastic lymphoma with cross-lineage rearrangement.
- Published
- 2020
- Full Text
- View/download PDF
27. In vitro and in vivo effects of toceranib phosphate on canine osteosarcoma cell lines and xenograft orthotopic models.
- Author
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Sánchez-Céspedes R, Accornero P, Miretti S, Martignani E, Gattino F, Maniscalco L, Gola C, Iussich S, Martano M, Morello E, Buracco P, Aresu L, and Maria R
- Subjects
- Animals, Bone Neoplasms veterinary, Cell Line, Tumor drug effects, Cell Proliferation drug effects, Dogs, Heterografts, In Vitro Techniques, Mice, Treatment Outcome, Bone Neoplasms drug therapy, Indoles pharmacology, Osteosarcoma drug therapy, Pyrroles pharmacology
- Abstract
Canine osteosarcoma (OSA) is the most common primary malignant bone tumour in dogs, and it has a high metastatic rate and poor prognosis. Toceranib phosphate (TOC; Palladia, Zoetis) is a veterinary tyrosine kinase inhibitor that selectively inhibits VEGFR-2, PDGFRs and c-Kit, but its efficacy is not yet fully understood in the treatment of canine OSA. Here, we evaluated the functional effects of TOC on six OSA cell lines by transwell, wound healing and colony formation assays. Subsequently, two cell lines (Wall and Penny) were selected and were inoculated in mice by intrafemoral injection to develop an orthotopic xenograft model of canine OSA. For each cell line, 30 mice were xenografted; half of them were used as controls, and the other half were treated with TOC at 40 mg/kg body weight for 20 days. TOC inhibited cell growth of all cell lines, but reduced invasion and migration was only observed in Penny and Wall cell lines. In mice engrafted with Penny cells and subjected to TOC treatment, decreased tumour growth was observed, and PDGFRs and c-Kit mRNA were downregulated. Immunohistochemical analyses demonstrated a significant reduction of Ki67 staining in treated mice when compared to controls. The results obtained here demonstrate that TOC is able to slightly inhibit cell growth in vitro, while its effect is evident only in a Penny cell xenograft model, in which TOC significantly reduced tumour size and the Ki67 index without modifying apoptosis markers., (© 2019 John Wiley & Sons Ltd.)
- Published
- 2020
- Full Text
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28. Fenoldopam Mesylate: A Narrative Review of Its Use in Acute Kidney Injury.
- Author
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Noce A, Marrone G, Rovella V, Busca A, Gola C, Ferrannini M, and Di Daniele N
- Subjects
- Acute Kidney Injury metabolism, Humans, Kidney metabolism, Male, Treatment Outcome, Acute Kidney Injury drug therapy, Dopamine Agonists therapeutic use, Fenoldopam therapeutic use, Kidney drug effects, Receptors, Dopamine D1 agonists
- Abstract
Background: Fenoldopam mesylate is a selective agonist of DA-1 receptors. It is currently used for the in-hospital treatment of severe hypertension. DA-1 receptors have high density in renal parenchyma and for this reason, a possible reno-protective role of Fenoldopam mesylate was investigated., Methods: We examined all studies regarding the role of Fenoldopam mesylate in Acute Kidney Injury (AKI); particularly, those involving post-surgical patients, intensive care unit patients and contrastinduced nephropathy., Results: Fenoldopam mesylate was found to be effective in reducing the onset of postoperative AKI, when used before the development of the kidney damage. Positive results were also obtained in the management of intensive care unit patients with AKI, although the clinical studies investigated were few and conducted on small samples., Conclusion: Conflicting results were achieved in contrast-induced nephropathy., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
- Published
- 2019
- Full Text
- View/download PDF
29. PDGFR-α, PDGFR-β, VEGFR-2 and CD117 expression in canine mammary tumours and evaluation of the in vitro effects of toceranib phosphate in neoplastic mammary cell lines.
- Author
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Gattino F, Maniscalco L, Iussich S, Biasato I, Martano M, Morello E, Gola C, Millán Ruiz Y, Saeki N, Buracco P, Martín de Las Mulas J, and De Maria R
- Subjects
- Animals, Cell Line, Tumor drug effects, Dogs, Female, Proto-Oncogene Proteins c-kit metabolism, Receptor, Platelet-Derived Growth Factor alpha metabolism, Receptor, Platelet-Derived Growth Factor beta metabolism, Vascular Endothelial Growth Factor Receptor-2 metabolism, Dog Diseases drug therapy, Dog Diseases metabolism, Indoles pharmacology, Mammary Neoplasms, Animal drug therapy, Mammary Neoplasms, Animal metabolism, Pyrroles pharmacology
- Abstract
Canine mammary tumours (CMTs) are one of the most common malignancies in bitches. Platelet-derived growth factor receptor (PDGFR) α and β, vascular endothelial growth factor receptor-2 (VEGFR-2) and CD117 are tyrosine kinase receptors involved in several tumours and represent suitable targets for specific therapy with toceranib phosphate. The purpose of this study was to evaluate the expression of these receptors in the pathogenesis and progression of CMTs. PDGFRα, PDGFRβ, VEGFR-2 and CD117 were expressed in 46/83 (55.4 per cent), 33/83 (39.8 per cent), 46/83 (55.4 per cent) and 32/83 (38.5 per cent) of CMTs, respectively. Immunohistochemical results showed a statistically significant loss of PDGFRα and PDGFRβ expression in simple carcinomas compared with complex/mixed carcinomas. Protein expression by western blot revealed specific bands corresponding to PDGFRα and VEGFR-2 in 3/7 and in 1/7 cell lines. Moreover, in vitro treatment showed that toceranib phosphate weakly reduced cell proliferation in one canine mammary cell line. Before considering TKR inhibitors for possible therapeutic approaches, actually further studies are necessary to evaluate the effect of these drugs on CMTs in vivo., Competing Interests: Competing interests: None declared., (© British Veterinary Association (unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.)
- Published
- 2018
- Full Text
- View/download PDF
30. [Separation and identification by thin-layer chromatography of 5 thymoanaleptic drugs derived from dibenzoazepine].
- Author
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Viala A, Gouezo F, and Gola C
- Subjects
- Chromatography, Thin Layer, Antidepressive Agents analysis, Desipramine analysis, Dibenzazepines analysis, Imipramine analysis, Opipramol analysis
- Published
- 1969
- Full Text
- View/download PDF
31. [Identification of tricyclic antidepressants derived from imipramine by gas chromatography].
- Author
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Viala A, Cano JP, Gola C, and Gouezo F
- Subjects
- Desipramine isolation & purification, Methods, Antidepressive Agents isolation & purification, Chromatography, Gas, Dibenzazepines isolation & purification, Imipramine isolation & purification
- Published
- 1971
- Full Text
- View/download PDF
32. [Method for the detection of imipraminic antidepressants in urine by gas chromatography and thin layer chromatography].
- Author
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Viala A, Gola C, Cano JP, and Gouezo F
- Subjects
- Chromatography, Gas, Chromatography, Thin Layer, Desipramine urine, Imipramine urine, Methods, Antidepressive Agents urine, Dibenzazepines urine
- Published
- 1972
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