1. Rapid and quantitative detection of C-reactive protein based on quantum dots and immunofiltration assay
- Author
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Huiqi Lu, Huanxing Han, Mohamed Shehata Draz, Yan Bao, Chang Liu, and Pengfei Zhang
- Subjects
Analyte ,Hepatitis B virus ,Point-of-Care Systems ,Biophysics ,Analytical chemistry ,Pharmaceutical Science ,Bioengineering ,Polyethylene glycol ,Hepacivirus ,C-reactive proteins ,Polyethylene Glycols ,Biomaterials ,chemistry.chemical_compound ,Hepatitis B, Chronic ,International Journal of Nanomedicine ,Limit of Detection ,Drug Discovery ,PEG ratio ,Quantum Dots ,medicine ,Humans ,Original Research ,Detection limit ,Immunoassay ,Chromatography ,medicine.diagnostic_test ,Chemistry ,Organic Chemistry ,technology, industry, and agriculture ,PEGylation ,General Medicine ,Fluorescence ,Glutathione ,Hepatitis C ,C-Reactive Protein ,Quantum dot ,Glutathione capped QDs ,point-of-care test ,Filtration - Abstract
Pengfei Zhang,1,* Yan Bao,1,* Mohamed Shehata Draz,2,3,* Huiqi Lu,1 Chang Liu,1 Huanxing Han11Center for Translational Medicine, Changzheng Hospital, Second Military Medical University, Shanghai, People’s Republic of China; 2Zhejiang-California International Nanosystems Institute, Zhejiang University, Hangzhou, Zhejiang, People’s Republic of China; 3Faculty of Science, Tanta University, Tanta, Egypt*These authors contributed equally tothis workAbstract: Convenient and rapid immunofiltration assays (IFAs) enable on-site “yes” or “no” determination of disease markers. However, traditional IFAs are commonly qualitative or semi-quantitative and are very limited for the efficient testing of samples in field diagnostics. Here, we overcome these limitations by developing a quantum dots (QDs)-based fluorescent IFA for the quantitative detection of C-reactive proteins (CRP). CRP, the well-known diagnostic marker for acute viral and bacterial infections, was used as a model analyte to demonstrate performance and sensitivity of our developed QDs-based IFA. QDs capped with both polyethylene glycol (PEG) and glutathione were used as fluorescent labels for our IFAs. The presence of the surface PEG layer, which reduced the non-specific protein interactions, in conjunction with the inherent optical properties of QDs, resulted in lower background signal, increased sensitivity, and ability to detect CRP down to 0.79 mg/L with only 5 µL serum sample. In addition, the developed assay is simple, fast and can quantitatively detect CRP with a detection limit up to 200 mg/L. Clinical test results of our QD-based IFA are well correlated with the traditional latex enhance immune-agglutination aggregation. The proposed QD-based fluorescent IFA is very promising, and potentially will be adopted for multiplexed immunoassay and in field point-of-care test.Keywords: C-reactive proteins, point-of-care test, Glutathione capped QDs, PEGylation
- Published
- 2015