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Rapid and quantitative detection of C-reactive protein based on quantum dots and immunofiltration assay
- Source :
- International Journal of Nanomedicine
- Publication Year :
- 2015
-
Abstract
- Pengfei Zhang,1,* Yan Bao,1,* Mohamed Shehata Draz,2,3,* Huiqi Lu,1 Chang Liu,1 Huanxing Han11Center for Translational Medicine, Changzheng Hospital, Second Military Medical University, Shanghai, People’s Republic of China; 2Zhejiang-California International Nanosystems Institute, Zhejiang University, Hangzhou, Zhejiang, People’s Republic of China; 3Faculty of Science, Tanta University, Tanta, Egypt*These authors contributed equally tothis workAbstract: Convenient and rapid immunofiltration assays (IFAs) enable on-site “yes” or “no” determination of disease markers. However, traditional IFAs are commonly qualitative or semi-quantitative and are very limited for the efficient testing of samples in field diagnostics. Here, we overcome these limitations by developing a quantum dots (QDs)-based fluorescent IFA for the quantitative detection of C-reactive proteins (CRP). CRP, the well-known diagnostic marker for acute viral and bacterial infections, was used as a model analyte to demonstrate performance and sensitivity of our developed QDs-based IFA. QDs capped with both polyethylene glycol (PEG) and glutathione were used as fluorescent labels for our IFAs. The presence of the surface PEG layer, which reduced the non-specific protein interactions, in conjunction with the inherent optical properties of QDs, resulted in lower background signal, increased sensitivity, and ability to detect CRP down to 0.79 mg/L with only 5 µL serum sample. In addition, the developed assay is simple, fast and can quantitatively detect CRP with a detection limit up to 200 mg/L. Clinical test results of our QD-based IFA are well correlated with the traditional latex enhance immune-agglutination aggregation. The proposed QD-based fluorescent IFA is very promising, and potentially will be adopted for multiplexed immunoassay and in field point-of-care test.Keywords: C-reactive proteins, point-of-care test, Glutathione capped QDs, PEGylation
- Subjects :
- Analyte
Hepatitis B virus
Point-of-Care Systems
Biophysics
Analytical chemistry
Pharmaceutical Science
Bioengineering
Polyethylene glycol
Hepacivirus
C-reactive proteins
Polyethylene Glycols
Biomaterials
chemistry.chemical_compound
Hepatitis B, Chronic
International Journal of Nanomedicine
Limit of Detection
Drug Discovery
PEG ratio
Quantum Dots
medicine
Humans
Original Research
Detection limit
Immunoassay
Chromatography
medicine.diagnostic_test
Chemistry
Organic Chemistry
technology, industry, and agriculture
PEGylation
General Medicine
Fluorescence
Glutathione
Hepatitis C
C-Reactive Protein
Quantum dot
Glutathione capped QDs
point-of-care test
Filtration
Subjects
Details
- ISSN :
- 11782013
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- International journal of nanomedicine
- Accession number :
- edsair.doi.dedup.....43c82b2fc4f8068231464af3d7e95955