1. Olfactory marker protein (OMP) regulates formation and refinement of the olfactory glomerular map
- Author
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Prateek Agarwal, Allison C. Provost, Venkatesh N. Murthy, Dinu F. Albeanu, Edward R. Soucy, and Joseph D. Zak
- Subjects
0301 basic medicine ,Science ,General Physics and Astronomy ,Biology ,Receptors, Odorant ,urologic and male genital diseases ,complex mixtures ,Olfactory Receptor Neurons ,Article ,General Biochemistry, Genetics and Molecular Biology ,Genetic Heterogeneity ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Olfactory Marker Protein ,medicine ,Animals ,lcsh:Science ,Receptor ,Alleles ,Uncategorized ,Mice, Knockout ,Glomerulus (olfaction) ,Multidisciplinary ,urogenital system ,Genetic heterogeneity ,General Chemistry ,respiratory system ,Immunohistochemistry ,Olfactory Bulb ,Mice, Mutant Strains ,Sensory neuron ,Olfactory bulb ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,Odor ,nervous system ,biology.protein ,bacteria ,lcsh:Q ,sense organs ,Signal transduction ,Olfactory marker protein ,030217 neurology & neurosurgery - Abstract
Inputs from olfactory sensory neuron (OSN) axons expressing the same type of odorant receptor (OR) converge in the glomerulus of the main olfactory bulb. A key marker of mature OSNs is olfactory marker protein (OMP), whose deletion has been associated with deficits in OSN signal transduction and odor discrimination. Here, we investigate glomerular odor responses and anatomical architecture in mice in which one or both alleles of OMP are replaced by the fluorescent synaptic activity reporter, synaptopHluorin. Functionally heterogeneous glomeruli, that is, ones with microdomains with distinct odor responses, are rare in OMP+/– mice, but occur frequently in OMP–/– mice. Genetic targeting of single ORs reveals that these microdomains arise from co-innervation of individual glomeruli by OSNs expressing different ORs. This glomerular mistargeting is locally restricted to a few glomerular diameters. Our studies document functional heterogeneity in sensory input within individual glomeruli and uncover its anatomical correlate, revealing an unexpected role for OMP in the formation and refinement of the glomerular map., Olfactory marker protein (OMP) expressed in all olfactory sensory neurons (OSN) is required for proper signal transduction and odor discrimination. Here, the authors report that OMP deletion leads to formation of glomeruli with axons from heterogeneous OSNs due to local axonal mistargeting.
- Published
- 2023
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