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We used the trait composition of macroinvertebrate communities to identify the effects of pesticides and multiple stressors associated with urban land use at different sites of four rivers in Spain. Several physical and chemical stressors (high metal pollution, nutrients, elevated temperature and flow alterations) affected the urban sites. The occurrence of multiple stressors influenced aquatic assemblages at 50% of the sites. We hypothesized that the trait composition of macroinvertebrate assemblages would reflect the strategies that the assemblages used to cope with the respective environmental stressors. We used RLQ and fourth corner analysis to address the relationship between stressors and the trait composition of benthic macroinvertebrates. We found a statistically significant relationship between the trait composition and the exposure of assemblages to environmental stressors. The first RLQ dimension, which explained most of the variability, clearly separated sites according to the stressors. Urban-related stressors selected taxa that were mainly plurivoltine and fed on deposits. In contrast, pesticide impacted sites selected taxa with high levels of egg protection (better egg survival), indicating a potentially higher risk for egg mortality. Moreover, the trait diversity of assemblages at urban sites was low compared to that observed in pesticide impacted sites, suggesting the homogenization of assemblages in urban areas. © 2017 The Authors, This study has been supported by the EU FP7 project GLOBAQUA [Grant Agreement No. 603629], the NET-Scarce project [Redes de Excelencia CTM2015-69780-REDC]?and by the Generalitat de Catalunya [Consolidated Research Groups: 2014 SGR 418?Water and Soil Quality Unit and 2014 SGR 291?ICRA]. MK acknowledges AGAUR fellowship of the Generalitat de Catalunya.?Special thanks to Laura Armend?riz for the identification of the?Oligochaeta.

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The concept of resilience has become popular in many disciplines far beyond its original use in the field of ecology. Despite of its wide use, it has received different definitions not always coincident. Such ambiguity is still more evident in its quantitative characterization. Most of the available methods are heavily context dependent and often difficult to apply in the practice. Here, we propose to define and calculate resilience starting from the data matrices resulting from multivariate measurements of different biological metrics. • The resilience between two field scenarios (each one characterized by their corresponding datasets) can be conveniently captured as the difference between its respective data complexities. • Complexity is quantified by means of the entropy associated to the spectral distribution of the singular values of each data matrix. • The method proposed has been illustrated with a case study in which the resilience of a river (Ebro River, NE Spain) is calculated comparing six biological metrics associated to the phytoplankton, upstream and downstream to a series of large reservoirs that alter the natural river flow regime. © 2019 The Author(s), This study has been financially supported by the EU FP7 project GLOBAQUA [Grant Agreement No. 603629 ] and by the Generalitat de Catalunya [Consolidated Research Groups: 2017 SGR 01404-Water and Soil Quality Unit].

In this paper, we build a preliminary inventory of dissolved phase water emissions of 36 of the 45 chemical priority substances under the European Union's Water Framework Directive. For point sources, we consider the European Pollutant Release and Transfer Register (E-PRTR) containing reported emissions from major industrial facilities. We consider all other sources as diffuse, and we estimate European average chemical emission factors from available measurements of dissolved phase concentrations, assuming simple emission patterns such as population and agricultural land. The emission inventory enables modelling concentrations, which have been compared with independent measurements. Due to the way they are estimated, they cannot withstand a point-by-point comparison. However, predicted concentrations exhibit a frequency distribution and order of magnitude compatible with observations, and match a fair proportion of independently reported exceedances of environmental quality standards for many of the substances studied. While apparently a preliminary picture based on crude simplifications, our representation suggests that simple drivers such as population and agriculture are useful to describe chemical pollution at European scale. From our preliminary inventory, E-PRTR industrial point emissions seem to account for a relatively small share of total emissions. Consequently, apart from specific measures such as upgrades to urban wastewater treatment plants in certain high impact areas, the management of priority substances may require a more strategic approach to emission control, addressing chemical use across sectors and the management of out-phased, legacy chemicals. At the same time, we advocate that improving emission inventories requires monitoring data reflecting the variability of emission patterns across Europe, as presently available monitoring data do not enable a catchment-specific estimation of emissions. © 2019 The Authors, This study was partly funded by, and developed in the context of Project Globaqua within the EU Seventh Framework Programme for research, technological development and demonstration under grant agreement No 603629 . Emission factors presented in this paper were estimated by Rafael Marcé within the scope of a service contract of ICRA for the JRC (ref. 392653) funded under the Danube Water Nexus cluster of activities of the JRC in support to the EU Strategy for the Danube region. The IPChem platform is developed and maintained at the European Commission's DG JRC under the responsibility of S. Kephalopoulos. We wish to particularly thank European Commission colleagues Silvia Dalla Costa for her generous and effective support with IPChem data, and Helen Clayton for her advice on an early version of this paper. Appendix A

Temporary rivers become dependent on wastewater effluent for base flows, which severely impacts river ecosystems through exposure to elevated levels of nutrients, dissolved organic matter, and organic micropollutants. However, biodegradation processes occurring in these rivers can be enhanced by wastewater bacteria/biofilms. Here, we evaluated the attenuation of pharmaceuticals and their human metabolites performing retrospective analysis of 120 compounds (drugs, their metabolites and transformation products) in mesocosm channels loaded with wastewater effluents twice a week for a period of 31 days. Eighteen human metabolites and seven biotransformation products were identified with high level of confidence. Compounds were classified into five categories. Type-A: recalcitrant drugs and metabolites (diclofenac, carbamazepine and venlafaxine); Type-B: degradable drugs forming transformation products (TPs) (atenolol, sitagliptin, and valsartan); Type-C: drugs for which no known human metabolites or TPs were detected (atorvastatin, azithromycin, citalopram, clarithromycin, diltiazem, eprosartan, fluconazole, ketoprofen, lamotrigine, lormetazepam, metformin, telmisartan, and trimethoprim); Type-D: recalcitrant drug metabolites (4-hydroxy omeprazole sulfide, erythro/threo-hydrobupropion, and zolpidem carboxylic acid); Type-E: unstable metabolites whose parent drug was not detectable (norcocaine, benzolylecgonine, and erythromycin A enol ether). Noteworthy was the valsartan acid formation from valsartan with transient formation of TP-336., This study has been financially supported by the EU through the PRIMA project (INWAT 201980E121). This work was supported by the Spanish Ministry of Science and Innovation (Project CEX2018-000794-S). The authors also acknowledge SCIEX for providing the loan instrument LC/HRMS QTOF X500R. The EU is not liable for any use that may be made of the information contained therein.

Wastewater discharges from cities and industries, especially megacities, and intensive livestock can be considered as main sources of pollution of our rivers and groundwater. Water pollution, therefore, constitutes a major threat to both aquatic ecosystems and human health. Here we address the influence of chemical pollution in waste- and drinking water, their associated potential toxicological effects, as well as, the available technologies for their removal. This opinion paper provides illustrative selected examples covering a broad range for both drinking water and wastewater treatment processes, for which a battery of toxicity tests is applied for their risk assessment. The examples are classified based on five hot topics: (i) Bioassays for toxicity evaluation, (ii) Toxicity of municipal wastewaters, (iii) Toxicity of pharmaceutical residues and hospital wastewaters, (iv) Toxicity of other non-urban effluent examples, and (v) Drinking water treatment processes and toxicity evaluation. 'Chemical analysis combined with batteries of bioassays covering a broad range of endpoints: cytotoxicity, endocrine disruption, genotoxicity, and other types seem to be good way to assess performance/efficiency of the water treatment processes when removing chemical contaminants.. Altogether, while recognizing that water treatment is a cornerstone for water pollution reduction, providing safe water for both human use and its return back to the aquatic environment will be undoubtedly enhanced with the use of ecotoxicity biomonitoring., This work has been financially supported by the Generalitat de Catalunya (Consolidated Research Group “2017 SGR 1404 - Water and Soil Quality Unit”) and by the Spanish Ministry of Science and Innovation (Project CEX2018-000794-S). The authors would like to thank the reviewers whose valuable comments helped us to improve the manuscript.

To meet the United Nations (UN) sustainable development goals and the European Union (EU) strategy for a non-toxic environment, water resources and ecosystems management require cost-efficient solutions for prevailing complex contamination and multiple stressor exposures. For the protection of water resources under global change conditions, specific research needs for prediction, monitoring, assessment and abatement of multiple stressors emerge with respect to maintaining human needs, biodiversity, and ecosystem services. Collaborative European research seems an ideal instrument to mobilize the required transdisciplinary scientific support and tackle the large-scale dimension and develop options required for implementation of European policies. Calls for research on minimizing society’s chemical footprints in the water–food–energy–security nexus are required. European research should be complemented with targeted national scientific funding to address specific transformation pathways and support the evaluation, demonstration and implementation of novel approaches on regional scales. The foreseeable pressure developments due to demographic, economic and climate changes require solution-oriented thinking, focusing on the assessment of sustainable abatement options and transformation pathways rather than on status evaluation. Stakeholder involvement is a key success factor in collaborative projects as it allows capturing added value, to address other levels of complexity, and find smarter solutions by synthesizing scientific evidence, integrating governance issues, and addressing transition pathways. This increases the chances of closing the value chain by implementing novel solutions. For the water quality topic, the interacting European collaborative projects SOLUTIONS, MARS and GLOBAQUA and the NORMAN network provide best practice examples for successful applied collaborative research including multi-stakeholder involvement. They provided innovative conceptual, modelling and instrumental options for future monitoring and management of chemical mixtures and multiple stressors in European water resources. Advancement of EU water framework directive-related policies has therefore become an option. © 2019, The Author(s)., Funding text #1 This article has been prepared as an outcome of the projects SOLUTIONS (European Union’s Seventh Framework Programme for research, technological development and demonstration under Grant Agreement No. 603437), GLOBAQUA (European Union’s Seventh Framework Programme for research, technological development and demonstration under Grant Agreement No. 603629) and MARS (European Union’s Seventh Framework Programme for research, technological development and demonstration under Grant Agreement No. 603378). Funding text #2 The authors declare that they have no competing interests. CIS Common Implementation Strategy DG Directorate General DPSIR Drivers–Pressure–State–Impacts–Response ECHA European Chemical Agency EEA European Environmental Agency EFSA European Food Safety Authority EU European Union ICPDR International Commission for the Protection of the Danube River ICPR International Commission for the Protection of the Rhine NGO Non-Governmental Organisation SDG sustainable development goals UN United Nations WFD Water Framework Directive

Environmental water quality monitoring aims to provide the data required for safeguarding the environment against adverse biological effects from multiple chemical contamination arising from anthropogenic diffuse emissions and point sources. Here, we integrate the experience of the international EU-funded project SOLUTIONS to shift the focus of water monitoring from a few legacy chemicals to complex chemical mixtures, and to identify relevant drivers of toxic effects. Monitoring serves a range of purposes, from control of chemical and ecological status compliance to safeguarding specific water uses, such as drinking water abstraction. Various water sampling techniques, chemical target, suspect and non-target analyses as well as an array of in vitro, in vivo and in situ bioanalytical methods were advanced to improve monitoring of water contamination. Major improvements for broader applicability include tailored sampling techniques, screening and identification techniques for a broader and more diverse set of chemicals, higher detection sensitivity, standardized protocols for chemical, toxicological, and ecological assessments combined with systematic evidence evaluation techniques. No single method or combination of methods is able to meet all divergent monitoring purposes. Current monitoring approaches tend to emphasize either targeted exposure or effect detection. Here, we argue that, irrespective of the specific purpose, assessment of monitoring results would benefit substantially from obtaining and linking information on the occurrence of both chemicals and potentially adverse biological effects. In this paper, we specify the information required to: (1) identify relevant contaminants, (2) assess the impact of contamination in aquatic ecosystems, or (3) quantify cause–effect relationships between contaminants and adverse effects. Specific strategies to link chemical and bioanalytical information are outlined for each of these distinct goals. These strategies have been developed and explored using case studies in the Danube and Rhine river basins as well as for rivers of the Iberian Peninsula. Current water quality assessment suffers from biases resulting from differences in approaches and associated uncertainty analyses. While exposure approaches tend to ignore data gaps (i.e., missing contaminants), effect-based approaches penalize data gaps with increased uncertainty factors. This integrated work suggests systematic ways to deal with mixture exposures and combined effects in a more balanced way, and thus provides guidance for future tailored environmental monitoring. © 2019, The Author(s)., Funding text #1 1 UFZ‑Helmholtz Centre for Environmental Research, Permoserstr. 15, 04318 Leipzig, Germany. 2 Institute for Environmental Research, RWTH Aachen University, 52074 Aachen, Germany. 3 Office of Research and Development, Atlantic Ecology Division, United States Environmental Protection Agency, Narragansett, RI, USA. 4 Center for Applied Geoscience, Eberhard Karls Uni‑ versity Tübingen, 72074 Tübingen, Germany. 5 Alterra, Wageningen University and Research Centre, P.O. Box 47, 6700 AA Wageningen, The Netherlands. 6 Environment and Climate Change Canada, Burlington, ON, Canada. 7 Sophus Bauditz Vej 19 B, 2800 Kgs. Lyngby, Denmark. 8 Water and Soil Quality Research Group, Institute of Environmental Assessment and Water Research (IDAEA‑ CSIC), Jordi Girona 18‑26, 08034 Barcelona, Spain. 9 Unité d’Ecotoxicologie Funding text #2 The SOLUTIONS Project is supported by the Seventh Framework Programme (FP7‑ENV‑2013) of the European Union under Grant Agreement No. 603437. G.A. Umbuzeiro thanks FAPESP Projects 2013/16956‑6 and 2015/24758‑5. We like to thank all partners for their continued efforts in making this project a success story. Funding text #3 The SOLUTIONS Project is supported by the Seventh Framework Programme (FP7‑ENV‑704 2013) of the European Union under Grant Agreement No. 603437. G.A. Umbuzeiro thanks 705 FAPESP Projects 2013/16956‑6 and 2015/24758‑5.

Urban wastewater inputs are a relevant pollution source to rivers, contributing a complex mixture of nutrients, organic matter and organic microcontaminants to these systems. Depending on their composition, WWTP effluents might perform either as enhancers (subsidizers) or inhibitors (stressors) of biological activities. In this study, we evaluated in which manner biofilms were affected by treated urban WWTP effluent, and how much they recovered after exposure was terminated. We used indoor artificial streams in a replicated regression design, which were operated for a total period of 56 days. During the first 33 days, artificial streams were fed with increasing concentration of treated effluents starting with non-contaminated water and ending with undiluted effluent. During the recovery phase, the artificial streams were fed with unpolluted water. Sewage effluents contained high concentrations of personal care products, pharmaceuticals, nutrients, and dissolved organic matter. Changes in community structure, biomass, and biofilm function were most pronounced in those biofilms exposed to 58% to 100% of WWTP effluent, moving from linear to quadratic or cubic response patterns. The return to initial conditions did not allow for complete biofilm recovery, but biofilms from the former medium diluted treatments were the most benefited (enhanced response), while those from the undiluted treatments showed higher stress (inhibited response). Our results indicated that the effects caused by WWTP effluent discharge on biofilm structure and function respond to the chemical pressure only in part, and that the biofilm dynamics (changes in community composition, increase in thickness) imprint particular response pathways over time. © 2019 Elsevier B.V., This study has been financially supported by the EU FP7 project GLOBAQUA [Grant Agreement No. 603629] and by the Generalitat de Catalunya [Consolidated Research Groups: 2017 SGR 01404−Water and Soil Quality Unit]. NM thanks SCIEX for generously providing the loaned instrument. Authors are grateful to Vicenç Acuña, Maria Casellas, Ferran Romero and Olatz Pereda for their support and advice during the experiment, and to Peter Eichhorn for his critical reading of the manuscript.

Degradation of solid polymers in the aquatic environment encompasses a variety of biotic and abiotic processes giving rise to heterogeneous patterns across the surface of the material, which cannot be investigated using conventional Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) that only renders an "average" picture of the sample. In that context, MALDI-TOF MS Imaging (MALDI MSI) provides a rapid and efficient tool to study 2D spatial changes occurred in the chemical composition of the polymer surface.Commercial polycaprolactone diol (average molecular weight of 1250 Da) was selected as test material because it had been previously known to be amenable to biological degradation. The test oligomer probe was incubated under aerobic and denitrifying conditions using synthetic water and denitrifying mixed liquor obtained from a wastewater treatment plant respectively. After ca. seven days of exposure the mass spectra obtained by MALDI MSI showed the occurrence of chemical modifications in the sample surface. Observed heterogeneity across the probe's surface indicated significant degradation and suggested the contribution of biotic processes. The results were investigated using different image processing tools. Major changes on the oligomer surface were observed when exposed to denitrifying conditions. © 2016., This study has been financially supported by the EU through the FP7 project GLOBAQUA (Grant agreement No 603629 ), and by the Generalitat de Catalunya (Consolidated Research Groups “2014 SGR 418—Water and Soil Quality Unit” and "2014 SGR 291—ICRA"). It reflects only the author's views. The Community is not liable for any use that may be made of the information contained therein. The authors are thankful to Dr. P. Eichhorn for his critical reading of the manuscript and the valuable comments provided and to Maria Casellas (Experimental Streams Facility, Catalan Institute for Water Research, Girona, Spain) for her technical help in the operation of the artificial stream. Appendix A

Characterization of river ecosystems must take into consideration both structural and functional aspects. For the latter, a convenient and simple approach for routine monitoring is based on the decomposition of organic matter measured in terms of breakdown of natural organic substrates like leaf litter, wood sticks. Here we extended the method to a synthetic organic material using polymer probes characterized by MALDI-TOF/MS. We first characterized several commercial available polymers, and finally selected polycaprolactonediol 1250 (PCP 1250), a polyester oligomer, as the most convenient for further studies. PCP 1250 was first tested at mesocosms scale under conditions simulating those of the river, with and without nutrient addition for up to 4 weeks. Differences to the starting material measured in terms of changes in the relative ion peak intensities were clearly observed. Ions exhibited a different pattern evolution along time depending on their mass. Greatest changes were observed at longest exposure time and in the nutrient addition treatment. At shorter times, the effect of nutrients (addition or not) was indistinguishable. Finally, we performed an experiment in 11 tributaries of the Ebro River during 97 days of exposure. Principal Component Analysis confirmed the different behavior of ions, which were clustered according to their mass. Exposed samples were clearly different to the standard starting material, but could not be well distinguished among each other. Polymer mass loss rates, as well as some environmental variables such as conductivity, temperature and flow were correlated with some peak intensities. Overall, the interpretation of field results in terms of environmental conditions remains elusive, due to the influence of multiple concurrent factors. Nevertheless, breakdown of synthetic polymers opens an interesting field of research, which can complement more traditional breakdown studies to assess river ecosystem functioning. © 2016 The Authors, This study has been financially supported by the EU through the FP7 project GLOBAQUA (Grant agreement No 603629 ), and by the Generalitat de Catalunya (Consolidated Research Groups “ 2014 SGR 418 —Water and Soil Quality Unit” and 2014 SGR 291 —ICRA). It reflects only the author's views. The Community is not liable for any use that may be made of the information contained therein. The authors are thankful to Maria Casellas (Experimental Streams Facility, Catalan Institute for Water Research, Girona, Spain) for her technical help in the operation of the artificial streams. Appendix A

Los traductores de las dos obras fueron Antonio de Ginebreda y Jacobo de Benavente, respectivamente El pie de imp. consta en colofón Juan Varela de Salamanca, aunque imprimió principalmente en Sevilla, lo hizo también en Toledo entre 1510 y 1514 Sign: a-g\p6\s, A-E\p6\s Port. con grab. xil. representando a San Jerónimo Port. con orla de cuatro piezas Texto a dos col

Expuesto en: Un tesoro en la Universidad de Sevilla : Incunables y obras de los siglos XVI y XVII : Exposición, noviembre-diciembre 1993 L. gót. --2 tam. --2 col. --45-46 lín. --Inic. grab.