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During clinical procedures, the lithotripter shock wave (LSW) that is incident on the stone and resultant stress field is often asymmetric due to the respiratory motion of the patient. The variations of the LSW-stone interaction and associated fracture pattern were investigated by photoelastic imaging, phantom experiments, and three-dimensional fluid-solid interaction modeling at different lateral locations in a lithotripter field. In contrast to a T-shaped fracture pattern often observed in the posterior region of the disk-shaped stone under symmetric loading, the fracture pattern gradually transitioned to a tilted L-shape under asymmetric loading conditions. Moreover, the model simulations revealed the generation of surface acoustic waves (SAWs), i.e., a leaky Rayleigh wave on the anterior boundary and Scholte wave on the posterior boundary of the stone. The propagation of SAWs on the stone boundary is accompanied by a progressive transition of the LSW reflection pattern from regular to von Neumann and to weak von Neumann reflection near the glancing incidence and, concomitantly, the development and growth of a Mach stem, swirling around the stone boundary. The maximum tensile stress and stress integral were produced by SAWs on the stone boundary under asymmetric loading conditions, which drove the initiation and extension of surface cracks into the bulk of the stone that is confirmed by micro-computed tomography analysis.

Passive cavitation mapping, which generates images using bubble acoustic emission signals, has been increasingly used for monitoring and guiding focused ultrasound surgery. Passive cavitation mapping can be used as an adjunct to magnetic resonance imaging to provide crucial information on the safety and efficacy of focused ultrasound surgery. The most widely used algorithm for passive cavitation mapping is delay-and-sum. One of the major limitations of delay-and-sum is its suboptimal computational efficiency. Although frequency-domain delay-and-sum can partially resolve this issue, such an algorithm is not suitable for imaging the evolution of bubble activity in real time and for cases in which cavitation events occur asynchronously. This study investigates a transient angular spectrum approach for passive cavitation mapping. The working principle of this approach is to back propagate the received signal to the domain of interest and reconstruct the spatial-temporal wave field encoded with the bubble location and collapse time. The transient angular spectrum approach is validated using an in silico model and water bath experiments. It is found that the transient angular spectrum approach yields similar results to delay-and-sum, but it is one order of magnitude faster. The results obtained by this study suggest that the transient angular spectrum approach is promising for fast and accurate passive cavitation mapping.

OBJECTIVE: To investigate the mechanism of stone dusting in Holmium (Ho): YAG laser lithotripsy (LL). MATERIALS AND METHODS: Cylindrical BegoStone samples (6 × 6 mm, H × D) were treated in water using a clinical Ho:YAG laser lithotripter in dusting mode (0.2–0.4 J with 70–78 μs in pulse duration, 20 Hz) at various fiber tip to stone standoff distances (SD = 0, 0.5, and 1 mm). Stone damage craters were quantified by optical coherence tomography and bubble dynamics were captured by high-speed video imaging. To differentiate the contribution of cavitation vs thermal ablation to stone damage, three additional experiments were performed. First, presoaked wet stones were treated in air to assess stone damage without cavitation. Second, the laser fiber was advanced at various offset distances (OSD = 0.25, 1, 2, 3, and 10 mm) from the tip of a flexible ureteroscope to alter the dynamics of bubble collapse. Third, stones were treated with parallel fiber to minimize photothermal damage while isolating the contribution of cavitation to stone damage. RESULTS: Treatment in water resulted in 2.5- to 90-fold increase in stone damage compared with those produced in air where thermal ablation dominates. With the fiber tip placed at OSD = 0.25 mm, the collapse of the bubble was distracted away from the stone surface by the ureteroscope tip, leading to significantly reduced stone damage compared with treatment without the scope or with scope at large OSD of 3–10 mm. The average crater volume produced by parallel fiber orientation at 0.2 J after 100 pulses, where cavitation is the dominant mechanism of stone damage, was comparable with those produced by using perpendicular fiber orientation within SD = 0.25–1 mm. CONCLUSION: Cavitation plays a dominant role over photothermal ablation in stone dusting during short pulse Ho:YAG LL when 10 or more pulses are delivered to the same location.

The penetration depth of photoacoustic imaging in biological tissues has been fundamentally limited by the strong optical attenuation when light is delivered externally through the tissue surface. To address this issue, we previously reported internal-illumination photoacoustic imaging using a customized radial-emission optical fiber diffuser, which, however, has complex fabrication, high cost, and non-uniform light emission. To overcome these shortcomings, we have developed a new type of low-cost fiber diffusers based on a graded-scattering method in which the optical scattering of the fiber diffuser is gradually increased as the light travels. The graded scattering can compensate for the optical attenuation and provide relatively uniform light emission along the diffuser. We performed Monte Carlo numerical simulations to optimize several key design parameters, including the number of scattering segments, scattering anisotropy factor, divergence angle of the optical fiber, and reflective index of the surrounding medium. These optimized parameters collectively result in uniform light emission along the fiber diffuser and can be flexibly adjusted to accommodate different applications. We fabricated and characterized the prototype fiber diffuser made of agarose gel and intralipid. Equipped with the new fiber diffuser, we performed thorough proof-of-concept studies on ex vivo tissue phantoms and an in vivo swine model to demonstrate the deep-imaging capability (~10 cm achieved ex vivo) of photoacoustic tomography. We believe that the internal light delivery via the optimized fiber diffuser is an effective strategy to image deep targets (e.g., kidney) in large animals or humans.

Recent studies indicate that cavitation may play a vital role in laser lithotripsy. However, the underlying bubble dynamics and associated damage mechanisms are largely unknown. In this study, we use ultra-high-speed shadowgraph imaging, hydrophone measurements, three-dimensional passive cavitation mapping (3D-PCM), and phantom test to investigate the transient dynamics of vapor bubbles induced by a holmium:yttrium aluminum garnet laser and their correlation with solid damage. We vary the standoff distance ( SD) between the fiber tip and solid boundary under parallel fiber alignment and observe several distinctive features in bubble dynamics. First, long pulsed laser irradiation and solid boundary interaction create an elongated “pear-shaped” bubble that collapses asymmetrically and forms multiple jets in sequence. Second, unlike nanosecond laser-induced cavitation bubbles, jet impact on solid boundary generates negligible pressure transients and causes no direct damage. A non-circular toroidal bubble forms, particularly following the primary and secondary bubble collapses at SD = 1.0 and 3.0 mm, respectively. We observe three intensified bubble collapses with strong shock wave emissions: the intensified bubble collapse by shock wave, the ensuing reflected shock wave from the solid boundary, and self-intensified collapse of an inverted “triangle-shaped” or “horseshoe-shaped” bubble. Third, high-speed shadowgraph imaging and 3D-PCM confirm that the shock origins from the distinctive bubble collapse form either two discrete spots or a “smiling-face” shape. The spatial collapse pattern is consistent with the similar BegoStone surface damage, suggesting that the shockwave emissions during the intensified asymmetric collapse of the pear-shaped bubble are decisive for the solid damage.

Purpose: Although cavitation during laser lithotripsy (LL) contributes to the Moses effect, the impact of cavitation on stone damage is less clear. Using different laser settings, we investigate the role of cavitation bubbles in energy delivery and stone damage. Materials and Methods: The role of cavitation in laser energy delivery was characterized by using photodetector measurements synced with high-speed imaging for laser pulses of varying durations. BegoStone samples were treated with the laser fiber oriented perpendicularly in contact with the stone in water or in air to assess the impact of cavitation on crater formation. Crater volume and geometry were quantified by using optical coherence tomography. Further, the role of cavitation in stone damage was elucidated by treatment in water with the fiber oriented parallel to the stone surface and by photoelastic imaging. Results: Longer pulse durations resulted in higher energy delivery but smaller craters. Stones treated in water resulted in greater volume, wider yet shallower craters compared with those treated in air. Stones treated with the parallel fiber showed crater formation after 15 pulses, confirmed by high-speed imaging of the bubble collapse with the resultant stress field captured by photoelastic imaging. Conclusions: Despite improved energy delivery, the longer pulse mode produced smaller crater volume, suggesting additional processes secondary to photothermal ablation are involved in stone damage. Our critical observations of the difference in stone damage treated in water vs in air, combined with the crater formation by parallel fiber, suggest that cavitation is a contributor to stone damage during LL.

Shock wave lithotripsy (SWL) has been widely used for non-invasive treatment of kidney stones. Cavitation plays an important role in stone fragmentation, yet it may also contribute to renal injury during SWL. It is therefore crucial to determine the spatiotemporal distributions of cavitation activities to maximize stone fragmentation while minimizing tissue injury. Traditional cavitation detection methods include high-speed optical imaging, active cavitation mapping (ACM), and passive cavitation mapping (PCM). While each of the three methods provides unique information about the dynamics of the bubbles, PCM has most practical applications in biological tissues. To image the dynamics of cavitation bubble collapse, we previously developed a sliding-window PCM (SW-PCM) method to identify each bubble collapse with high temporal and spatial resolution. In this work, to further validate and optimize the SW-PCM method, we have developed tri-modality cavitation imaging that includes three-dimensional high-speed optical imaging, ACM, and PCM seamlessly integrated in a single system. Using the tri-modality system, we imaged and analyzed laser-induced single cavitation bubbles in both free field and constricted space and shock wave-induced cavitation clusters. Collectively, our results have demonstrated the high reliability and spatial-temporal accuracy of the SW-PCM approach, which paves the way for the future in vivo applications on large animals and humans in SWL.

A damage model suggested by the Tuler–Butcher concept of dynamic accumulation of microscopic defects is obtained from experimental data on microcrack formation in synthetic kidney stones. Experimental data on appearance of microcracks is extracted from micro-computed tomography images of BegoStone simulants obtained after subjecting the stone to successive pulses produced by an electromagnetic shock-wave lithotripter source. Image processing of the data is used to infer statistical distributions of crack length and width in representative transversal cross-sections of a cylindrical stone. A high-resolution finite volume computational model, capable of accurately modeling internal reflections due to local changes in material properties produced by material damage, is used to simulate the accumulation of damage due to successive shocks. Comparison of statistical distributions of microcrack formation in computation and experiment allows calibration of the damage model. The model is subsequently used to compute fracture of a different aspect-ratio cylindrical stone predicting concurrent formation of two main fracture areas as observed experimentally.

Temperature mapping is essential in many biomedical studies and interventions to precisely control the tissue’s thermal conditions for optimal treatment efficiency and minimal side effects. Based on the Grüneisen parameter’s temperature dependence, photoacoustic (PA) imaging can provide relative temperature measurement, but it has been traditionally challenging to measure absolute temperatures without knowing the baseline temperature, especially in deep tissues with unknown optical and acoustic properties. Here, we report a new thermal-energy-memory-based photoacoustic thermometry (TEMPT). By illuminating the tissue with a burst of nanosecond laser pulses, TEMPT exploits the temperature dependence of the thermal energy lingering, which is probed by the corresponding PA signals acquired within the thermal confinement. A self-normalized ratiometric measurement cancels out temperature-irrelevant quantities and estimates the Grüneisen parameter. The temperature can then be evaluated, given the tissue’s temperature-dependent Grüneisen parameter, mass density, and specific heat capacity. Unlike the conventional PA thermometry, TEMPT does not require the knowledge of tissue’s baseline temperature, nor the optical properties. We have developed a mathematical model to describe the temperature dependence in TEMPT. We have demonstrated the feasibility of the temperature evaluation on tissue phantoms at 1.5 cm depth within a clinically relevant temperature range. Finally, as proof-of-concept, we applied TEMPT for temperature mapping during focused ultrasound treatment in mice in vivo at 2 mm depth. As a generic temperature mapping method, TEMPT is expected to find applications in thermotherapy of cancers on small animal models.

A multiphysics computational model of the focusing of an acoustic pulse and subsequent shock wave formation that occurs during extracorporeal shock wave lithotripsy is presented. In the electromagnetic lithotripter modeled in this work the focusing is achieved via a polystyrene acoustic lens. The transition of the acoustic pulse through the solid lens is modeled by the linear elasticity equations and the subsequent shock wave formation in water is modeled by the Euler equations with a Tait equation of state. Both sets of equations are solved simultaneously in subsets of a single computational domain within the BEARCLAW framework which uses a finite-volume Riemann solver approach. This model is first validated against experimental measurements with a standard (or original) lens design. The model is then used to successfully predict the effects of a lens modification in the form of an annular ring cut. A second model which includes a kidney stone simulant in the domain is also presented. Within the stone the linear elasticity equations incorporate a simple damage model.

In this manuscript, we first describe the fabrication protocol of a microfluidic chip, with gold dots and fibronectin-coated regions on the same glass substrate, that precisely controls the generation of tandem bubbles and individual cells patterned nearby with well-defined locations and shapes. We then demonstrate the generation of tandem bubbles by using two pulsed lasers illuminating a pair of gold dots with a few-microsecond time delay. We visualize the bubble-bubble interaction and jet formation by high-speed imaging and characterize the resultant flow field using particle image velocimetry (PIV). Finally, we present some applications of this technique for single cell analysis, including cell membrane poration with macromolecule uptake, localized membrane deformation determined by the displacements of attached integrin-binding beads, and intracellular calcium response from ratiometric imaging. Our results show that a fast and directional jetting flow is produced by the tandem bubble interaction, which can impose a highly localized shear stress on the surface of a cell grown in close proximity. Furthermore, different bioeffects can be induced by altering the strength of the jetting flow by adjusting the standoff distance from the cell to the tandem bubbles.

In shock wave lithotripsy (SWL), acoustic pulses with a leading compression wave followed by a tensile wave are delivered into the patient’s body using a water-filled coupling cushion. Cavitation-related acoustic energy loss in the coupling unit depends critically on water conditions, e.g. dissolved gas concentration and exchange flow rate. We have systematically investigated the attenuation mechanism in the coupling water via pressure measurements and cavitation characterization. In non-degassed water the bubble cluster became progressively dense (i.e., proliferated because of gas diffusion into bubbles and splitting of bubbles into many daughter bubbles) in shock waves delivered at 1 Hz leading to reduction in the tensile wave duration from a nominal value of 4.6 to 1.8 µs. To reduce cavitation in the coupling water along the beam path, we have used a continuous jet flow to remove residual daughter bubbles between consecutive shocks. As a result, stone fragmentation efficiency was increased from 16±4% t...

Carefully timed tandem microbubbles have been shown to produce directional and targeted membrane poration of individual cells in microfluidic systems, which could be of use in ultrasound-mediated drug and gene delivery. This study aims at contributing to the understanding of the mechanisms at play in such an interaction. The dynamics of single and tandem microbubbles between two parallel plates is studied numerically and analytically. Comparisons are then made between the numerical results and the available experimental results. Numerically, assuming a potential flow, a three-dimensional boundary element method (BEM) is used to describe complex bubble deformations, jet formation, and bubble splitting. Analytically, compressibility and viscous boundary layer effects along the channel walls, neglected in the BEM model, are considered while shape of the bubble is not considered. Comparisons show that energy losses modify the bubble dynamics when the two approaches use identical initial conditions. The initial conditions in the boundary element method can be adjusted to recover the bubble period and maximum bubble volume when in an infinite medium. Using the same conditions enables the method to recover the full dynamics of single and tandem bubbles, including large deformations and fast re-entering jet formation. This method can be used as a design tool for future tandem-bubble sonoporation experiments.

Previous studies have demonstrated that stone comminution decreases with increased pulse repetition frequency as a result of bubble proliferation in the cavitation field of a shock wave lithotripter (Pishchalnikov et al 2011 J. Acoust. Soc. Am. 130 EL87-93). If cavitation nuclei remain in the propagation path of successive lithotripter pulses, especially in the acoustic coupling cushion of the shock wave source, they will consume part of the incident wave energy, leading to reduced tensile pressure in the focal region and thus lower stone comminution efficiency. We introduce a method to remove cavitation nuclei from the coupling cushion between successive shock exposures using a jet of degassed water. As a result, pre-focal bubble nuclei lifetime quantified by B-mode ultrasound imaging was reduced from 7 to 0.3 s by a jet with an exit velocity of 62 cm s(-1). Stone fragmentation (percent mass2 mm) after 250 shocks delivered at 1 Hz was enhanced from 22 ± 6% to 33 ± 5% (p = 0.007) in water without interposing tissue mimicking materials. Stone fragmentation after 500 shocks delivered at 2 Hz was increased from 18 ± 6% to 28 ± 8% (p = 0.04) with an interposing tissue phantom of 8 cm thick. These results demonstrate the critical influence of cavitation bubbles in the coupling cushion on stone comminution and suggest a potential strategy to improve the efficacy of contemporary shock wave lithotripters.

The Nanopulse Lithotripter (NPL; Lithotech Medical, Israel) is a novel intracorporeal device that uses a nanosecond duration electrical discharge through a reusable flexible coaxial probe to endoscopically fragment urinary stones. This device was compared with a holmium laser lithotripsy (HoL) with regard to stone fragmentation efficiency (SFE) and its impact on flexible ureteroscope (URS) deflection and flow of irrigation.Using a custom bench model, a 6 mm BegoStone cylindrical phantom (mixture 5:2) was confined under 0.9% saline atop sequential mesh sieves. The SFE of two NPL probe sizes (2.0F, 3.6F) and two HoL fibers (200, 365 μm) was evaluated using concordant settings of 1 J and 5 Hz. URS deflection and irrigation flow with NPL probes in the working channel were tested in five new fourth generation flexible URS and compared with other adjunct endourologic instruments.The 2.0F NPL showed improved SFE compared with the 200 μm laser (86 mg/min vs 52 mg/min, p = 0.014) as did the 3.6F NPL vs the 365 μm laser (173 mg/min vs 80 mg/min, p = 0.05). The NPL created more 1 to 2 mm fragments; the laser created more dust. URS deflection reduced by 3.75° with the 2.0 NPL probe. URS irrigation flow reduced from 36.5 to 6.3 mL/min with the 2.0F NPL probe.NPL shows improved SFE compared with HoL. Flow with the 2.0F probe is akin to a stone basket. NPL offers an effective alternative to HoL.

INTRODUCTION AND OBJECTIVES: A new intracorporeal lithotriptor uses a nanosecond duration electrical discharge through a flexible coaxial probe to endoscopically fragment urinary stones. This device was compared to a holmium laser with regards to stone fragmentation efficiency and their impact on flexible ureteroscope deflection and flow of irrigation. METHODS: Using a custom bench model, stone fragmentation efficiency of the Nano-electric Pulse Lithotriptor (NPL, Lithotech Group, Israel) was evaluated with a begostone (mixture 5:2) confined under 0.9% normal saline atop sequential mesh sieves. Two NPL probe sizes (2.0 and 3.6F) and two holmium laser fibers (200 mm and 365mm) were evaluated using 6mm begostones. Time to first break, time to complete break (all fragments

The dynamics, stabilization, and acoustic spectra of a bubble cluster in different liquids are investigated under the condition of ultrasonic cavitation. Experimental data for the dynamics of a spherical ultrasonic cluster near the end face of a rod, capillary, or pressure sensor placed in the antinode of a pressure standing wave at the center of a single-wave spherical piezoelectric concentrator (piezoelectric sphere) are presented. The variation of the cluster size with the parameters of the ultrasonic field and properties of the liquid is studied. It is found that the shape, collapse dynamics, and stability of the cavitation cluster have a significant influence on the acoustocapillary effect. It is shown that the maximal acoustocapillary effect and sonoluminescence are observed when a stable cluster with spherically symmetric collapse dynamics is provided at the end of a capillary in a 50% solution of glycerol. Using a small-size piezotransducer placed at the center of the sphere, the acoustic pressure is measured and acoustic spectra are studied for different voltages across the piezosphere and during the formation of variously shaped cavitation clusters. In the case of fully developed cavitation and a spherical cluster, the acoustic spectra contain subharmonic components, the cavitation noise factor rises to 35%, and the maximum of the noise envelope shifts toward higher frequencies.

In this study, a foam mask is introduced to elongate the beam width in the focal plane of a shock wave lithotripter, and a respiration gating beam steering strategy is proposed to accurately target stones, which are movable because of patient respiratory motion, radiation force, hydraulics, gravity, etc. The mask is steered to revolve the elongated elliptical focal zone of the acoustic field, so as to cover as much as possible the stone moving area. The beam steering strategy provides the most opportunity to deliver the shock wave energy straightly on the moving stones but not to the peripheral tissues, enhancing the stone fracture efficacy whilst reducing tissue injury. Simulation results with specific stone motion models are given to proof the advantages of the elongated focal zone in tracking stone target, and show that the stone overlap in the focal zone is improved significantly and competitively with the beam steering strategy, compared with no beam steering.

Kidney stones are a common and painful affliction, but fortunately physics offers some relief: Sound can be used to break up the stones, without invasive surgery. Efficacy of this ``lithotripsy'' treatment, however, depends strongly on cavitation, which can both attenuate the sound waves and provoke significant tissue damage. In this collaboration between a physicist, engineers, and materials scientists, our understanding of this process is advanced through measurements and modeling, providing insight into the relations between bubble dynamics and lithotripsy effectiveness.

The mechanism responsible for the development of autocyclic circular breakdowns in electrolyte as a result of the electric discharge within a hole in a dielectric plate has been experimentally studied. It is demonstrated that the breakdowns are caused by an axisymmetric electrohydrodynamic process involving the formation of a circular bubble breakdown, which acts as a current interrupter in the stage of expansion and as a switch in the stage of collapse, which is followed by the circular breakdown. On the whole, a self-adjusting antiphase process of bubble generation dynamics is developed in the system, whereby each preceding bubble collapses, in the field of pressure due to the subsequent bubble breakdown. This collapse proceeds at an accelerated rate and is accompanied by the fractionation of bubbles.

The early stage of the dynamics of interacting cavitation bubbles in a transmitted spherically focused pulsed wave consisting of compression and rarefaction phases is studied. The cavitation is investigated away from the liquid boundaries for negative pressures up to −42 MPa with a decrease rate of −40 MPa/μs by high-speed microscopic filming (100 million frames per second and a spatial resolution of 5–50μm/pixel) and pressure measurements. It is demonstrated that, according to the kinematics of size variation, the bubbles are separated into two fractions: expanding and collapsing ones. Experimental data provide grounds to assume that the formation of a two-fraction bubble cluster occurs on account of different threshold values of pressure in the rarefaction wave for two characteristic sizes of nuclei, namely, micronuclei (d I < 10μm) and nanonuclei (“bubstons,” d II ∼ 1 nm), which become detectable only when the rarefaction wave amplitude exceeds the critical one. Pulsed compression of small bubbles in the process of transformation of a rarefaction wave into a compression wave occurs under the effect of a cluster’s internal pressure up to 20 MPa and proceeds with the conservation of the spherical shape of the second-fraction bubbles. It is demonstrated that the velocity of the center of mass of a bubble reaches its peak value close to the moment of bubble collapse. The translational and radial dynamics of a bubble are studied in a numerical experiment using the Rayleigh-Plesset equation and taking into account the viscous force and the pressure gradient. The results of measuring the translational shift may be useful for estimating the minimum bubble radius by comparing the numerical results with the experiment.

To elucidate mechanisms of cavitation action on a surface with microcapillary discontinuity and refine the model of the sonocapillary effect, the force acting on a cylinder is correlated with the height of a liquid in the capillary under cavitation, both quantities being measured at the same point of the ultrasonic field. It is found that the force acting on the cylinder is directed toward a cavitation cluster stabilized at the end face of the cylinder. This force can be enhanced with another cylinder placed on the side of the cluster coaxially with the first one. The dynamics of the cavitation cluster depending on the cylinder spacing is investigated in the case when one of the cylinders is on a pendulum suspension. The conditions for self-sustained oscillations of the pendulum are found. The dependence of the attracting force between the cylinders on the ultrasonic frequency and cylinder spacing is derived. The end-face-averaged pressure exerted on a cylinder of diameter 1.2 mm may reach 0.16 kPa, and the intracapillary pressure attains 0.89 kPa. Thus, the sonocapillary effect may be explained, at least partially, by a counterpressure arising from cluster-capillary interaction. This effect can be used in designing cavitation sensors and ultrasonic sources.

The determination of the phase of the bubble oscillation at the instant of light emission, which is a key issue for understanding the origin of cavitation luminescence of liquids, is discussed. The observation of luminescence in the course of the nucleation and growth of a bubble up to its collapse is performed in a bipolar wave consisting of a compression phase followed by a rarefaction phase in the regime of a two-fraction bubble cluster formation. The space-time distributions of the luminescence intensity and pressure and the dynamics of the cluster in water and a glycerin solution are investigated at the early stage of cavitation. A correlation between the maximal density of light flashes and the positive pressure pulses in the field of superposition of the initial and secondary cavitation compression waves is revealed. It is shown that the spherical focusing of acoustic pulses both away from the boundaries of the liquid and near its free surface makes it possible to compare the luminescence intensities for different rates of the pressure decrease.

The application of an electric discharge at numerous holes in a dielectric diaphragm for generating spherically focused shock waves in a salt solution is described. The design of the presented transducer makes the erosion of the electrodes impossible, since they are placed beyond the discharge zone; as a result, the point of focus stabilizes. The dependences on the discharge voltage, the number and total area of the holes in the diaphragm, and the electrolyte concentration are obtained for the pressure-wave amplitude at the focus; the extreme values are ∼40 and –12 MPa in the compression and rarefaction waves, respectively. The possibility of comminuting stones with masses of

Nonlinear processes accompanying the focusing of a microsecond acoustic pulse produced by an electromagnetic source shaped as a spherical segment are investigated. The processes are considered to be far from the boundaries of a liquid, in the absence of cavitation. Detailed measurements of the pressure field by a fiber-optic sensor and high-speed photography of the shock front are performed. The pressure field is found to be determined by the nonlinear effects that occur in the course of the propagation of the initial converging compression wave and an edge rarefaction wave. The peak pressure amplitudes at the focus are 75 and −42 MPa for the compression and rarefaction waves, respectively, at the maximum voltage of the pulse generator in use. The measured length of the compression wave front is equal to the response time of the sensor (8 ns). The pressure amplitude is shown to be limited by the irregularity of the propagation of a shock wave in the form of Mach’s disk. At the focus, the pressure gradient across the radiator axis reaches 0.5 atm/μm, while the diameter of the focal spot is 2.5±0.2 mm. The focus of the edge rarefaction wave formed due to diffraction is located closer to the radiator than the focus of the compression wave, which may facilitate the study of the biological effect of cavitation independently of the shear motion of the medium.

A modified version of a flat electromagnetic converter for enhancing the amplitude of the pressure of a shock wave using the Laptev-Trishin method and reducing the duration of an acoustic pulse is described. The interference of a focused edge diffraction rarefaction wave and a wave reflected from a free surface is used to form a narrow space-time negative-pressure region. The conditions for generating a single cavitation bubble near the free surface are found.

A transducer for measuring the velocity of shock wave propagation in salt water is described. The transducer operation is based on the depolarization of a double electrical layer exposed to an acoustic pulse. The time lag of the electrolysis current step at a disk anode was used for measuring the velocity of the propagation of the pressure disturbance. It was shown experimentally that the suggested method provides a measurement accuracy of 2% for the shock-wave front velocity, and base length of 5 mm. The transducer withstands repeated exposure to cavitation and shock waves at a pressure of up to 70 MPa.

The efficiency of shock wave lithotripsy (SWL), a noninvasive first-line therapy for millions of nephrolithiasis patients, has not improved substantially in the past two decades, especially in regard to stone clearance. Here, we report a new acoustic lens design for a contemporary electromagnetic (EM) shock wave lithotripter, based on recently acquired knowledge of the key lithotripter field characteristics that correlate with efficient and safe SWL. The new lens design addresses concomitantly three fundamental drawbacks in EM lithotripters, namely, narrow focal width, nonidealized pulse profile, and significant misalignment in acoustic focus and cavitation activities with the target stone at high output settings. Key design features and performance of the new lens were evaluated using model calculations and experimental measurements against the original lens under comparable acoustic pulse energy (E+) of 40 mJ. The −6-dB focal width of the new lens was enhanced from 7.4 to 11 mm at this energy level, and peak pressure (41 MPa) and maximum cavitation activity were both realigned to be within 5 mm of the lithotripter focus. Stone comminution produced by the new lens was either statistically improved or similar to that of the original lens under various in vitro test conditions and was significantly improved in vivo in a swine model (89% vs. 54%, P = 0.01), and tissue injury was minimal using a clinical treatment protocol. The general principle and associated techniques described in this work can be applied to design improvement of all EM lithotripters.

The dynamics of luminescence generation and cavitation in the field of spherically focused and plane shock-acoustic waves in water, glycerin, and their mixtures is studied. It is shown that in the spherical focusing of shock-acoustic waves, the first pulse of luminescence correlates with cavitational rupture of the liquid. It is assumed that the registered luminescence is caused by electrokinetic processes upon formation and growth of cavitational bubbles.

A resonant plasma coiwerter for an atomie particie analyzer, created for an open magnetic trap AMBAL-M, is presented. A flow of charge-exchange atoms from the main plasma passes through a hydrogen plasma target, where they undergo partially the resonant charge-exchange and are transformed into posilive ions. High efficiency of the charge conversion in a wide energy range is reached, pollution of the main plasma by heavy impurities is excluded, the analyzed particles do not lose energy on acauiring the electric charge. The formed ion flow is analyzed for energy by a 30° electrostatic analyzer, and after accelerations are registered by a multichannel deteetor made on the MCP basis. Experimental researches of the plasma converter have shown that the chosen plasma are source providcs a controlled change of the target linear density in the range of (0.2–2) 1014cm−2 at low ion temperature and Frank Condon atomie concentration of hydrogen. It permits to reach high transformation efficiency of atoms into...

The acoustic lens of the Modularis electromagnetic shock wave lithotripter (Siemens, Malvern, Pennsylvania) was modified to produce a pressure waveform and focal zone more closely resembling that of the original HM3 device (Dornier Medtech, Wessling, Germany). We assessed the newly designed acoustic lens in vivo in an animal model.Stone fragmentation and tissue injury produced by the original and modified lenses of the Modularis lithotripter were evaluated in a swine model under equivalent acoustic pulse energy (about 45 mJ) at 1 Hz pulse repetition frequency. Stone fragmentation was determined by the weight percent of stone fragments less than 2 mm. To assess tissue injury, shock wave treated kidneys were perfused, dehydrated, cast in paraffin wax and sectioned. Digital images were captured every 120 μm and processed to determine functional renal volume damage.After 500 shocks, the mean ± SD stone fragmentation efficiency produced by the original and modified lenses was 48% ± 12% and 52% ± 17%, respectively (p = 0.60). However, after 2,000 shocks, the modified lens showed significantly improved stone fragmentation compared to the original lens (mean 86% ± 10% vs 72% ± 12%, p = 0.02). Tissue injury caused by the original and modified lenses was minimal at a mean of 0.57% ± 0.44% and 0.25% ± 0.25%, respectively (p = 0.27).With lens modification the Modularis lithotripter demonstrates significantly improved stone fragmentation with minimal tissue injury at a clinically relevant acoustic pulse energy. This new lens design could potentially be retrofitted to existing lithotripters, improving the effectiveness of electromagnetic lithotripters.

An experimental system for digital stereoscopic imaging produced by using a high-speed color camera is described. Two bright-field image projections of a three-dimensional object are captured utilizing additive-color backlighting (blue and red). The two images are simultaneously combined on a two-dimensional image sensor using a set of dichromatic mirrors, and stored for off-line separation of each projection. This method has been demonstrated in analyzing cavitation bubble dynamics near boundaries. This technique may be useful for flow visualization and in machine vision applications.

The performance of a newly developed light spot hydrophone (LSHD) in lithotripter field characterization was compared to that of the fiber optic probe hydrophone (FOPH). Pressure waveforms produced by a stable electromagnetic shock wave source were measured by the LSHD and FOPH under identical experimental conditions. In the low energy regime, focus and field acoustic parameters matched well between the two hydrophones. At clinically relevant high energy settings for shock wave lithotripsy, the measured leading compressive pressure waveforms matched closely with each other. However, the LSHD recorded slightly larger |P–| (p < 0.05) and secondary peak compressive pressures (p < 0.01) than the FOPH, leading to about 20% increase in total acoustic pulse energy calculated in a 6 mm radius around the focus (p = 0.06). Tensile pulse durations deviated ∼5% (p < 0.01) due to tensile wave shortening from cavitation activity using the LSHD. Intermittent compression spikes and laser light reflection artifacts have been correlated to bubble activity based on simultaneous high-speed imaging analysis. Altogether, both hydrophones are adequate for lithotripter field characterization as specified by the international standard IEC 61846.

Previous studies have demonstrated that treatment strategy plays a critical role in ensuring maximum stone fragmentation during shockwave lithotripsy (SWL). We aimed to develop an optimal treatment strategy in SWL to produce maximum stone fragmentation.Four treatment strategies were evaluated using an in-vitro experimental setup that mimics stone fragmentation in the renal pelvis. Spherical stone phantoms were exposed to 2100 shocks using the Siemens Modularis (electromagnetic) lithotripter. The treatment strategies included increasing output voltage with 100 shocks at 12.3 kV, 400 shocks at 14.8 kV, and 1600 shocks at 15.8 kV, and decreasing output voltage with 1600 shocks at 15.8 kV, 400 shocks at 14.8 kV, and 100 shocks at 12.3 kV. Both increasing and decreasing voltages models were run at a pulse repetition frequency (PRF) of 1 and 2 Hz. Fragmentation efficiency was determined using a sequential sieving method to isolate fragments less than 2 mm. A fiberoptic probe hydrophone was used to characterize the pressure waveforms at different output voltage and frequency settings. In addition, a high-speed camera was used to assess cavitation activity in the lithotripter field that was produced by different treatment strategies.The increasing output voltage strategy at 1 Hz PRF produced the best stone fragmentation efficiency. This result was significantly better than the decreasing voltage strategy at 1 Hz PFR (85.8% vs 80.8%, P=0.017) and over the same strategy at 2 Hz PRF (85.8% vs 79.59%, P=0.0078).A pretreatment dose of 100 low-voltage output shockwaves (SWs) at 60 SWs/min before increasing to a higher voltage output produces the best overall stone fragmentation in vitro. These findings could lead to increased fragmentation efficiency in vivo and higher success rates clinically.

The 3rd‐generation electromagnetic (EM) shock wave lithotripters often have narrow focal width and high peak pressure compared to the original Dornier HM‐3. In addition, the pressure waveform produced by a typical EM lithotripter has a secondary compressive wave following the tensile component that suppresses lithotripter pulse induced cavitation, which may impact negatively on stone comminution. These characteristic changes in the modern EM lithotripters may contribute in part to their reduced effectiveness observed clinically. To overcome these two drawbacks, we have designed a new acoustic lens for the Siemens Modularis EM lithotripter that produces an idealized pressure waveform similar to that of the HM‐3 with broad focal width and low peak pressure. At acoustic pulse energy of 53 mJ, the new lens design enlarges the −6 dB focal width of the Modularis by 47% while significantly reducing the second compressive wave in the lithotripter pulse throughout its focal plane. After 2000 shocks, in vitro commi...

The dynamic interaction between laser-generated tandem bubble and individual polystyrene particles of 2 and 10 μm in diameter is studied in a microfluidic channel (25 μm height) by high-speed imaging and particle image velocimetry. The asymmetric collapse of the tandem bubble produces a pair of microjets and associated long-lasting vortices that can propel a single particle to a maximum velocity of 1.4 m/s in 30 μs after the bubble collapse with a resultant directional displacement up to 60 μm in 150 μs. This method may be useful for high-throughput cell sorting in microfluidic devices.

A simple method for preparing artificial kidney stones with varying physical properties is described. BegoStone was prepared with a powder to water ratio (by weight) ranging from 15:3 to 15:6. The acoustic properties of the phantoms were characterized by using an ultrasound transmission technique, from which the corresponding mechanical properties were calculated based on elastic wave theory. The measured parameters for BegoStone phantoms of different water contents are: longitudinal wave speed (3148 – 4159 m/s), transverse wave speed (1813 – 2319 m/s), density (1563 – 1995 kg/m3), longitudinal acoustic impedance (4.92 – 8.30 Kg/m2*s), transverse acoustic impedance (2.83 – 4.63 Kg/m2*s), Young’s modulus (12.9 – 27.4 GPa), bulk modulus (8.6 – 20.2 GPa), and shear modulus (5.1 – 10.7 GPa), which cover the range of corresponding properties reported in natural kidney stones. In addition, diametral compression tests were carried out to determine tensile failure strength of the stone phantoms. BegoStone phantoms with varying water content at preparation have tensile failure strength from 6.9 – 16.3 MPa when tested dry and 3.2 – 7.1 MPa when tested in water-soaked condition. Overall, it is demonstrated that this new BegoStone preparation method can be used to fabricate artificial stones with physical properties matched with those of natural kidney stones of various chemical compositions.

The interaction of laser-generated tandem microbubble (maximum diameter of about $50\text{ }\text{ }\ensuremath{\mu}\mathrm{m}$) with single (rat mammary carcinoma) cells is investigated in a $25\mathrm{\text{\ensuremath{-}}}\ensuremath{\mu}\mathrm{m}$ liquid layer. Antiphase and coupled oscillation of the tandem microbubble leads to the formation of alternating, directional microjets (with max microstreaming velocity of $10\text{ }\text{ }\mathrm{m}/\mathrm{s}$) and vortices (max vorticity of $350\text{ }000\text{ }\text{ }{\mathrm{s}}^{\ensuremath{-}1}$) in opposite directions. Localized and directional membrane poration (200 nm to $2\text{ }\text{ }\ensuremath{\mu}\mathrm{m}$ in pore size) can be produced by the tandem microbubble in an orientation and proximity-dependent manner, which is absent from a single oscillating microbubble of comparable size and at the same stand-off distance.

Using a reflector insert, the original HM-3 lithotripter field at 20 kV was altered significantly with the peak positive pressure (p(+)) in the focal plane increased from 49 to 87 MPa while the -6 dB focal width decreased concomitantly from 11 to 4 mm. Using the original reflector, p(+) of 33 MPa with a -6 dB focal width of 18 mm were measured in a pre-focal plane 15-mm proximal to the lithotripter focus. However, the acoustic pulse energy delivered to a 28-mm diameter area around the lithotripter axis was comparable ( approximately 120 mJ). For all three exposure conditions, similar stone comminution ( approximately 70%) was produced in a mesh holder of 15 mm after 250 shocks. In contrast, stone comminution produced by the modified reflector either in a 15-mm finger cot (45%) or in a 30-mm membrane holder (14%) was significantly reduced from the corresponding values (56% and 26%) produced by the original reflector (no statistically significant differences were observed between the focal and pre-focal planes). These observations suggest that a low-pressure/broad focal width lithotripter field will produce better stone comminution than its counterpart with high-pressure/narrow focal width under clinically relevant in vitro comminution conditions.

The effect of beam size on stone comminution in the Dornier HM‐3 lithotripter were investigated using a modified reflector (MR), which was developed to produce an acoustic field with higher peak pressure and smaller beam size compared to the original reflector (OR) of the HM‐3. The acoustic fields produced at 20 kV by the MR in the focal plane, and by the OR in the focal and pre‐focal plane (z = −15 mm) were characterized using a light spot hydrophone. The efficiencies of stone comminution in a mesh holder were similar, but the corresponding values produced by using the MR in a membrane holder were significantly lower than those produced by the OR in the focal and pre‐focal planes. These results suggest that a broad beam size could increase stone comminution efficiency when fragments are spreading out or moving due to respiratory motion in a large area during SWL. In contrast, when stone fragments are confined and well aligned to lithotripter focus the beam size may not influence significantly the treatme...

To correlate thermal dose from focused ultrasound (FUS) with gene expression and tissue injury, a temperature plateau strategy was employed. Plasmids encoding luciferase gene under the control of hsp70B promoter were transfected into the right gastrocnemius muscle in a rat via electroporation. One day after transfection, hind limbs were treated with 3.3-MHz focused ultrasound, using one of four different temperature plateaus with spatial-peak time-average focal temperatures (T(SPTA)) of 46 degrees C, 48 degrees C, 51 degrees C and 62 degrees C. The treatment duration at the plateau temperature was varied from 0 to 30s. Gene expression was analyzed in vivo one day following FUS treatment, and HE staining was employed to assess tissue injury. Gene activation and tissue damage correlated closely with thermal dose. The highest level of gene activation was induced by FUS at T(SPTA)=51 degrees C for 20s, which was found to be statistically equivalent to that produced by water-bath hyperthermia.

The dynamic interaction of a shockwave (modelled as a pressure pulse) with an initially spherically oscillating bubble is investigated. Upon the shockwave impact, the bubble deforms non-spherically and the flow field surrounding the bubble is determined with potential flow theory using the boundary-element method (BEM). The primary advantage of this method is its computational efficiency. The simulation process is repeated until the two opposite sides of the bubble surface collide with each other (i.e. the formation of a jet along the shockwave propagation direction). The collapse time of the bubble, its shape and the velocity of the jet are calculated. Moreover, the impact pressure is estimated based on water-hammer pressure theory. The Kelvin impulse, kinetic energy and bubble displacement (all at the moment of jet impact) are also determined. Overall, the simulated results compare favourably with experimental observations of lithotripter shockwave interaction with single bubbles (using laser-induced bubbles at various oscillation stages). The simulations confirm the experimental observation that the most intense collapse, with the highest jet velocity and impact pressure, occurs for bubbles with intermediate size during the contraction phase when the collapse time of the bubble is approximately equal to the compressive pulse duration of the shock wave. Under this condition, the maximum amount of energy of the incident shockwave is transferred to the collapsing bubble. Further, the effect of the bubble contents (ideal gas with different initial pressures) and the initial conditions of the bubble (initially oscillating vs. non-oscillating) on the dynamics of the shockwave-bubble interaction are discussed.