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According to the latest scientific evidence, consumers appreciate hunted wild game meat (HWGM), but its consumption may expose them to some risks. Hunters produce HWGM, even if they may find it hard to identify themselves as food producers since in Western countries, hunting is practiced mostly as a leisure activity. Thus, hunters may underestimate the risk associated with HWGM handling, failing to preserve its safety during the production process. To test this hypothesis, our study aims to explore the presence of optimistic biases (OB) in a sample of Italian hunters. A sample of 408 hunters was asked to indicate their own risk and the perceived risk of their peers causing a foodborne disease to the final consumer, as well as the practices implemented during the phases of HWGM handling and preparation. Moreover, information about HWGM destinations, hunters' knowledge of basic principles of food safety, and risk perceptions of HWGM preparation were collected. Our results show that hunters generally tend to perceive themselves as "better than their peers" in performing actions to preserve HWGM safety, especially if the peers are unfamiliar to the respondent. Furthermore, OB correlates knowledge of basic principles of food safety with risk perceptions of HWGM preparation-related practices. Finally, hunters perceived themselves as "nature lovers" and "hunting enthusiasts" and did not recognize their role as food producers. Therefore, our findings emphasize the importance of improving hunters’ training to preserve public health, addressing the need for more targeted strategies able to enhance hunters’ awareness of their role as food producers.

In the US, newborn screening (NBS) is a unique health program that supports health equity and screens virtually every baby after birth, and has brought timely treatments to babies since the 1960's. With the decreasing cost of sequencing and the improving methods to interpret genetic data, there is an opportunity to add DNA sequencing as a screening method to facilitate the identification of babies with treatable conditions that cannot be identified in any other scalable way, including highly penetrant genetic neurodevelopmental disorders (NDD). However, the lack of effective dietary or drug-based treatments has made it nearly impossible to consider NDDs in the current NBS framework, yet it is anticipated that any treatment will be maximally effective if started early. Hence there is a critical need for large scale pilot studies to assess if and how NDDs can be effectively screened at birth, if parents desire that information, and what impact early diagnosis may have. Here we attempt to provide an overview of the recent advances in NDD treatments, explore the possible framework of setting up a pilot study to genetically screen for NDDs, highlight key technical, practical, and ethical considerations and challenges, and examine the policy and health system implications.

Newborn screening (NBS) for Severe Combined Immunodeficiency (SCID) by measurement of T-cell receptor excision circles (TRECs) successfully identifies newborns with SCID and severe T-cell lymphopenia, as intended. At the same time, NBS programs face the challenge of false positive results, with a disproportionately high number in the premature newborn population. This study evaluates TREC values and SCID screening outcomes in premature newborns and elucidates evidence-based SCID screening practices that reduce unnecessary follow-up activities in this population. De-identified individual SCID newborn screening data and aggregate SCID screening data were obtained from seven states across the US for babies born between 2018 and 2020. Relevant statistics were performed on data pooled from these states to quantify screening performance metrics and clinical impact on various birth and gestational age categories of newborns. The data were normalized using multiples-of-the-median (MoM) values to allow for the aggregation of data across states. The aggregation of NBS data across a range of NBS programs highlighted the trajectory of TREC values over time, both between and within newborns, and provides evidence for improved SCID screening recommendations in the premature and low birth weight population.

Marine operations that are required for the development and service of offshore wave energy farms represent a significant proportion of the total project costs. These operations can be optimised through design and innovation to improve the LCOE of the project. This paper presents an analysis of marine operations in offshore renewable energy projects and ows the importance of early, detailed analysis and optimisation of these activities. The analysis uses general-purpose techno-economic analysis software developed by Wave Venture. The software provides an integrated engineering and financial simulation specifically designed for the needs of offshore renewable energy technology. A 100 MW wave energy farm, made up of 250 CorPower devices, off the west coast of Ireland is defined and analysed to demonstrate the capabilities of the techno-economic analysis incorporating a marine operations logistics model. The results demonstrate the strength of integrated logistics and finance software in the analysis and design of wave farms, and how these simulations can lead to significant improvements in the LCOE of offshore renewable energy projects.

Each year, through population-based newborn screening (NBS), 1 in 294 newborns is identified with a condition leading to early treatment and, in some cases, life-saving interventions. Rapid advancements in genomic technologies to screen, diagnose, and treat newborns promise to significantly expand the number of diseases and individuals impacted by NBS. However, expansion of NBS occurs slowly in the United States (US) and almost always occurs condition by condition and state by state with the goal of screening for all conditions on a federally recommended uniform panel. The Newborn Screening Translational Research Network (NBSTRN) conducted the NBS Expansion Study to describe current practices, identify expansion challenges, outline areas for improvement in NBS, and suggest how models could be used to evaluate changes and improvements. The NBS Expansion Study included a workshop of experts, a survey of clinicians, an analysis of data from online repositories of state NBS programs, reports and publications of completed pilots, federal committee reports, and proceedings, and the development of models to address the study findings. This manuscript (Part One) reports on the design, execution, and results of the NBS Expansion Study. The Study found that the capacity to expand NBS is variable across the US and that nationwide adoption of a new condition averages 9.5 years. Four factors that delay and/or complicate NBS expansion were identified. A companion paper (Part Two) presents a use case for each of the four factors and highlights how modeling could address these challenges to NBS expansion.

Iron water-soluble porphyrins have been long used as biomimetic compounds for modelling the active sites found in heme-enzymes. In this regard, the anionic porphyrin [FeIII(TPPS)]3─ and its coordination complexes have been repeatedly chosen as suitable water-soluble platforms for bioinorganic chemistry studies. In this work we report for the first time the crystal structure of the water-soluble nitrosyl complex [FeII(TPPS) (NO•)]4─ along with that of oxodimeric ferric species [μ-O-([FeIII(TPPS)])2]8─.

Abstract Periurban farming systems are characterized by the need to adapt the farming practices coping with a modified natural and social environment. Questions are thus posed on the efficient use of the inputs. The purpose of this study is to estimate the technical efficiency and the productivity of periurban farms. To do so, the study employs a data envelopment analysis that properly captures the heterogeneity of the periurban farming system. The sample considered livestock and crop farms, located in the South Milan Agricultural Park, where 50 farms were selected and interviewed. Results show that crop farms are more efficient than livestock farms, but they have a less productive technology. The participation in short food supply chains and the multifunctional agriculture does not affect the levels of technical efficiency of the farms. Policies are thus needed to improve the education level of farmers and to sustain the efficiency of farms that diversify the farm’s economy.

Newborn screening (NBS) is a state or territory-based public health system that screens newborns for congenital diseases that typically do not present with clinical symptoms at birth but can cause significant mortality and morbidity if not detected or treated quickly. NBS continues to be one of the most successful public health interventions in the US, providing early detection and intervention to all infants. The increase in overall birth prevalence of core Recommended Uniform Screening Panel (RUSP) diseases detected via dried blood spot (DBS) specimens from 2015–2017 (17.50–18.31 per 10,000) to 2018–2020 (20.07 per 10,000), as reported into the APHL NewSTEPs database, affirms the importance and impact of NBS programs. This report presents aggregate numbers and birth prevalence of diseases detected by DBS on the RUSP from 2018–2020, including data from fifty US states and two territories.

The increase in meat consumption expected in the next decade will require more and more proteins for animal feeding. The recent amendments to the European “BSE Regulation” allow the use of insects and porcine-based meals in poultry farming, providing novel, sustainable substitutes for vegetable fodder. While the technological and nutritional properties of novel feeds containing processed animal proteins are widely recognized, far less is known about consumers’ acceptance of meat produced by animals fed on animal-based meals. In the present research, a best–worst survey was applied to estimate consumers’ preferences for chicken fed on plants, insects, or porcine-based meals using a sample of 205 Italian consumers. Furthermore, product price, type of farming, and “Free-from” labeling were considered in the analysis to evaluate the relative importance of feed ingredients compared to other important attributes of meats. The results show that the most relevant attributes are type of farming and “Free-from” claims, while type of feed represents the third attribute in order of importance. Notably, both insect and porcine flour are considered as negative characteristics of the product, suggesting that mandatory labeling signaling the use of these feeds would negatively impact on the value of chicken meat.

Although the impact of floods on the agricultural sector is relevant, with potential consequences on food security, in the new EU Common Agricultural Policy (CAP) proposal, agricultural risk management tools have been reinforced and extended. As far as we know, guidelines for the estimation of insurance indemnities related to flooding damage in the European livestock sector have not been proposed yet, unlike what has occurred in extra-European contexts. The present research proposes a model to identify the components of flood damage on dairy farms aimed at categorizing the cost typologies related to flood events by implementing a what-if approach. Our results highlight that collecting data about the vulnerability of a farm is an essential condition to assess the severity of damage from an economic perspective. In fact, even if some of the variables considered cause large economic losses per se, others are mainly related to poor management of issues related to the health of the herd (i.e., mastitis, lameness, other diseases). Such issues can be exacerbated by floods. Herd management, which includes comprehensive data collection, is essential for the calculation of economic losses in a single farm case and is also indispensable for the calculation of indemnity for the recovery of farming activities.

Cystic fibrosis (CF) newborn screening (NBS) was universally adopted in 2009 in the United States. Variations in NBS practices between states may impact the timing of diagnosis and intervention. Quantitative metrics can provide insight into NBS programs (NBSP), but the nuances cannot be elucidated without additional feedback from programs. This study was designed to determine facilitators and barriers to timely diagnosis and intervention following NBS for CF. The median age at the first CF event for infants with CF within each state was used to define early and late states (n = 15 per group); multiple CF centers were invited in states with more than two CF centers. Thirty states were eligible, and 61 NBSP and CF centers were invited to participate in structured interviews to determine facilitators and barriers. Once saturation of themes was reached, no other interviews were conducted. Forty-five interviews were conducted (n = 16 early CF center, n = 12 late CF center, n = 11 early NBSP, and n = 6 late NBSP). Most interviewees reported good communication between CF centers and NBSP. Communication between primary care providers (PCPs) and families was identified as a challenge, leading to delays in referral and subsequent diagnosis. The misperception of low clinical risk in infants from racial and ethnic minority groups was a barrier to early diagnostic evaluation for all groups. NBSP and CF centers have strong relationships. Early diagnosis may be facilitated through more engagement with PCPs. Quality improvement initiatives should focus on continuing strong partnerships between CF centers and NBS programs, improving education, communication strategies, and partnerships with PCPs, and improving CF NBS timeliness and accuracy.

In this paper, we investigate the short-term and long-term effects of the COVID-19 emergency on consumers' decision of changing dietary habit. We used a certified dataset reporting information about 456 Italian consumers during the lockdown in the first wave of the pandemic emergency (April 2020). The survey collected data about changes in food purchases, respondents' mood during the lockdown, conspiracist beliefs, exposure to the virus, and planned food purchasing behavior after the lockdown. We used the data to construct measures of the psychological pressure exerted by the COVID-19 emergency on consumers. We use an endogenous selection regression model to assess the impact of psychological pressure on the decision of changing food purchased. The analysis identified two opposite approaches to change in food purchasing decisions: impulsive approach and reflective approach. The former is associated with a higher probability of changing food purchase but a lower probability to keep the changes in the long run than the latter. Our results suggest that COVID-19 psychological pressure was associated with impulsive approach to buy food. Consequently, food-purchasing behavior is expected to revert to pre-COVID 19 habits when the emergency is over.

Newborn screening (NBS) is an essential public health service that performs screening to identify those newborns at increased risk for a panel of disorders, most of which are genetic. The goal of screening is to link those newborns at the highest risk to timely intervention and potentially life-saving treatment. The global COVID-19 pandemic led to disruptions within the United States public health system, revealing implications for the continuity of newborn screening laboratories and follow-up operations. The impacts of COVID-19 across different states at various time points meant that NBS programs impacted by the pandemic later could benefit from the immediate experiences of the earlier impacted programs. This article will review the collection, analysis, and dissemination of information during the COVID-19 pandemic facilitated by a national, centralized technical assistance and resource center for NBS programs.

The debate on climate change arose several concerns on the impacts of floods on agriculture and, consequently, on food security. At the same time, the European Floods Directive asks Members States to implement suitable measures to mitigate flood damage on economic activities, including farms. Still, while several analyses were developed to estimate potential flood damages on crops, a gap exists for livestock productions. The purpose of this study is to develop a conceptual model for the assessment of flood economic damages on dairy farms. Results propose a static and a dynamic model of farms recovery actions to re-establish the farming activity, which takes into account all farm components, as well as their interaction. Facing the destruction caused by floods, a first reaction of farmers could be closing the activity, with repercussion on this well-being as well as on farm workers’, and on the economy of rural areas. From this perspective, this study wants to provide a first methodological pathway to support farmers in restoring their activity.

Farmland values are driven by a complex set of factors. Starting from the idea that land rent values may reflect several characteristics both internal and external to agricultural sector, the paper has implemented a hedonic model based on land rent values in the metropolitan area of Milan, Northern Italy, assessing the influence of climate, soil, territorial and farm variables on a sample of farms. The model is based on data at rent contract level, matched with data at farm and municipal scale retrieved from different sources. Results confirm that land rent prices are affected by some climate variables, along with territorial and farm characteristics.

This study assesses the benefits and challenges of using genomics in Newborn Screening Programs (NBS) from the perspectives of State program officials. This project aims to help programs develop policies that will aid in the integration of genomic technology. Discussion groups were conducted with the NBS Program and Laboratory Directors in the seven HRSA Regional Genomics Collaboratives (August 2014–March 2016). The discussion groups addressed expected uses of genomics, potential benefits, and challenges of integrating genomic technology, and educational needs for parents and other NBS stakeholders: Twelve focus groups were conducted, which included participants from over 40 state programs. Benefits of incorporating genomics included improving screening modalities, supporting diagnostic procedures, and screening for a wider spectrum of disorders. Challenges included the costs of genomics, the ability to educate parents and health care providers about results, and the potential negative psychosocial impact of genomic information. Attempts to address the challenges of integrating genomics must focus on preserving the child welfare goals of NBS programs. Health departments will need to explore how genomics could be used to enhance programs while maintaining universal access to screening.

During the COVID-19 pandemic, state newborn screening programs faced challenges to ensure this essential public health program continued to function at a high level. In December 2020, the EveryLife Foundation for Rare Diseases held a workshop to discuss these common challenges and solutions. Newborn screening officials described challenges including short staffing across the entire program, collection and transport of specimens, interrupted follow-up activities, and pilot study recruitment. To address these challenges, state programs implemented a wide variety of solutions to maintain the high standards of newborn screening. To address staffing issues, newborn screening programs, public health laboratories, and hospitals all cross-trained personnel, worked to manage staff stress, and established essential functions. Other solutions included working with courier companies to ensure the timely pick-up of specimen, creating educational materials for hospital staff, and the creation of hybrid recruitment models for pilot studies. Implementing the lessons discussed throughout this paper can help to prepare for the next public health emergencies to ensure that a program that interacts with millions of families every year and saves the lives of thousands of children every year is minimally impacted.

To ensure the sustainable development of mountain livestock farming, the adequate remuneration of high-quality dairy products is fundamental. In this sense, communication strategies aimed at promoting mountain products and ensure better positioning and higher market prices are fundamental. The present research seeks to expand the literature regarding consumers’ willingness to pay for mountain foods by using an online real auction experiment aimed at evaluating the premium price that consumers are willing to pay for summer over winter mountain cheese, depending on the information provided concerning the taste anticipation or animal welfare. The results showed an overall small premium price given to the higher quality summer cheese; this could be, partially, due to a generally low degree of consumer knowledge about mountain dairy farming. With reference to communication strategies, the results provide evidence about the effectiveness of the rational messages founded upon sensorial characteristics and the anticipated taste of cheese. In addition, this study explored that adding a logo had no main effect on the price premium participants were willing to pay. This may be due to the fact that logos and claims, having a lower information content, are more indicated to lead the choice of consumers with a higher level of awareness. In the conclusion section, policy and agribusiness implications of the findings are provided.

Local production supports the economies of place and increasingly makes sense as the citizen-consumers increase in number and awareness. Nonetheless, despite the value of the short supply chains, some researchers have reacted sceptically to the irrational optimism around this sales structure. A close relationship with consumers does not imply more profit or exchange fairness by definition. In fact, increasing marketing costs must be considered and there is still information asymmetry, and the profiteering farmers could take advantage of the consumer trust. Through data reduction we explored the farmers' motivation and perceived effects of participating in short food supply chains. We also analysed the location of farms along with their size, production, sale channels and the relative market share, as well as whether they adopted quality certifications. We found that the farmers that work within the short food supply chains opt for a sort of co-certification mechanism based on the consumer/producer relationships rather than opting for the quality certification. Furthermore, the multivariate analysis showed different motivations and perceptions of direct sales among farmers: those that were the largest and farthest from the point of sale, were positive toward the social values of short food supply chains, while the rest seemed less competitive and were more motivated by profit and survival. The results reaffirm that the local production may not be good per se, and the presence of profit and surviving-orientation to market should be considered a treat especially for the reputation of the whole system.

The aim of the present research is to propose a new, quick and objective method for the certification of hunted and/or culled wild game meat quality and to monitor its origin and the hunting practices adopted by hunters. The expected deliverable is a new labelling scheme for Italian hunted wild game meat that will guarantee high quality and safety standards for consumers and will decrease transaction costs of the supply chains. During the 2015, 2016 and 2017 hunting seasons, 1,056 hunted wild ungulates were sampled. Specifically, alpine chamois (n=537), roe deer (n=113), red deer (n=342) and wild boar (n=64), which were all hunted in the VCO2-Ossola Nord hunting district (Verbania Province, Piedmont, Italy). Samples of the longissimus dorsi were collected to evaluate the nutritional parameters and the acid profiles of the products. As a measure of meat quality, pH values have been recorded after slaughtering by inserting a probe in the semimembranosus muscle. The results were categorized as DFD (pH≥6,2), intermediate DFD (5,8≤pH

For the economic sectors, the need to address the challenges posed by natural disasters due to climate change is an outstanding issue. To date, according to the European Commission (2019), there is still a gap in the estimation of the costs of flood in all European countries and the direct impact that these floods have on agricultural activities. More specifically, the damage to livestock has been minimally studied. The aim of this study is is therefore to identify the flood damage that affects dairy cattle farms, focusing on the damage to herds caused by a flood event; in fact, poor welfare conditions of dairy cattle directly affect production and thus farm revenue. To accomplish the aim of this study, a framework was first developed to identify possible damage types. Then, scientific literature focusing on the identification of flood damage to dairy herds was reviewed, and to quantify this damage to herds, literature sources providing information on the magnitude of variation in the identified damage types were used. Thus, our results provide relevant information on the variables that should be taken into account when assessing of the direct damage affecting the overall welfare of a dairy herd after a flood event. This evidence could then contribute to the development of tools aimed at assessing damage to dairy cattle on flood-affected farms.

The growing body of literature concerning the hunted wild game meat (HWGM) supply chain is mainly focused on the final consumer, while little is known about upstream production processes. Even though the hunter plays a central role here, it is not well understood how hunters themselves perceive their role in the various phases of the production process. The present study explores Italian hunters’ perception of the HWGM supply chain and compares it to their perception towards the conventional farmed meat supply chain. We distinguish several phases of this production process and find that the final phase related to on-site game dressing is considered problematic, perhaps because hunters perceive themselves as less skilled than professional butchers. The results, in fact, show that hunters prefer hunted products over farmed meat, but that they consider hunted wild boar meat less safe compared to farmed pork. Findings from this study provide a rare glimpse from the inside of the supply chain and reveals the needs for a broad risk assessment analysis on the Italian game meat supply chain. Considering the development of the Italian emerging market of the HWGM, our results also highlight the relevance of training activities on hunters in order to increase the safety and quality of the final product.

This study investigates potential areas for research and development in the feed sectors in Italy and Serbia. A questionnaire was submitted to 113 feed companies, 37% of these answered. Frequency statistics, data graphs and Simple Correspondence Analysis was obtained. Results indicated that 7% of responders in Italy and 47% of Serbia have not planned any budged for research and development in the last 3 years. The industrial processes were the main focus for Italy, while new product developments for Serbia. For both countries will be essential in the near future increase in product quality, search for new markets and reduction of energy consumption. Additionally, it was observed that company dimension is linked to the area/focus of innovation, irrespective of the type of feed production.

Acute brain injury leads to the recruitment and activation of immune cells including resident microglia and infiltrating peripheral myeloid cells (MC), which contribute to the inflammatory response involved in neuronal damage. We previously reported that TLR2 stimulation by peptidoglycan (PGN) from Staphylococcus aureus, in vitro and in vivo, induced microglial cell activation followed by autophagy induction. In this report, we evaluated if phosphatidyl-inositol-3 kinase (PI3K) pharmacological inhibitors LY294200 and 3-methyladenine (3-MA) can modulate the innate immune response to PGN in the central nervous system. We found that injection of PGN into the mouse brain parenchyma (caudate putamen) triggered an inflammatory reaction, which involved activation of microglial cells, recruitment of infiltrating MC to injection site, production of pro-inflammatory mediators, and neuronal injury. In addition, we observed the accumulation of LC3B+ CD45+ cells and colocalization of LC3B and lysosomal-associated membrane protein 1 in brain cells. Besides, we found that pharmacological inhibitors of PI3K, including the classical autophagy inhibitor 3-MA, reduced the recruitment of MC, microglial cell activation, and neurotoxicity induced by brain PGN injection. Collectively, our results suggest that PI3K pathways and autophagic response may participate in the PGN-induced microglial activation and MC recruitment to the brain. Thus, inhibition of these pathways could be therapeutically targeted to control acute brain inflammatory conditions.

Brain-resident microglia and peripheral migratory leukocytes play essential roles in shaping the immune response in the central nervous system. These cells activate and migrate in response to chemokines produced during active immune responses and may contribute to the progression of neuroinflammation. Herein, we addressed the participation of type I–II interferons in the response displayed by microglia and inflammatory monocytes to comprehend the contribution of these cytokines in the establishment and development of a neuroinflammatory process. Following systemic lipopolysaccharide (LPS) challenge, we found glial reactivity and an active recruitment of CD45hi leukocytes close to CD31+ vascular endothelial cells in circumventricular organs. Isolated CD11b+ CD45hi Ly6Chi Ly6G−-primed inflammatory monocytes were able to induce T cell proliferation, unlike CD11b+ CD45lo microglia. Moreover, ex vivo re-stimulation with LPS exhibited an enhancement of T cell proliferative response promoted by inflammatory monocytes. These myeloid cells also proved to be recruited in a type I interferon-dependent fashion as opposed to neutrophils, unveiling a role of these cytokines in their trafficking. Together, our results compares the phenotypic and functional features between tissue-resident vs peripheral recruited cells in an inflamed microenvironment, identifying inflammatory monocytes as key sentinels in a LPS-induced murine model of neuroinflammation.

Convenience foods, such as cured meats, have always been appreciated in terms of their price, convenience and of course their taste. The pig sector in Italy is substantially stable, and has recently been enhanced by new forms of product differentiation, such as quality certifications. We studied consumer preferences with respect to cured meats from organic farming using a Choice Experiment (CE) and Conditional Logit (CL) approach. In order to estimate consumer attitudes towards organic cured meats, we estimated the weight of externalities that such meats produce presenting them as intrinsic attributes of the product. The final aim was therefore to examine how better health, the protection of biodiversity and the reduction of pollution can affect the choices of organic versus conventional cured meats. Given the characteristics of CE, the trade-offs among these attributes and their value in terms of consumer willingness to pay (WTP) were thus estimated. The results indicate that consumers are well-disposed to organic cured meats, and are aware of the need to support livestock productions that respect the environment and animal welfare.

Newborn screening is a process-based public health service. Newborn screening staff and families alike are essential to maintaining the timeliness of the screening process. Newborn screening education must be accurate and accessible. Past newborn screening conferences have highlighted gaps in best practice and evidence-based guidance on newborn screening education. Sharing successful strategies across programs mitigates the scarcity of resources by cutting costs and reducing the burden of work. These factors illustrate the need for an education framework to guide newborn screening education efforts. The Newborn Screening Education Best Practices Framework responds to these issues by outlining guidance for newborn screening education approaches. Experts in the fields of newborn screening, genetics, and bioethics as well as previous research on best practice guidelines have contributed to the development of this framework. The framework outlines a process for users to evaluate newborn screening education approaches as best practices. This framework reviews best practices using a two-step approach, looking at guiding questions, implementation of the newborn screening issue, and evaluation. The framework helps the user define the characteristics of the newborn screening issue, intended audience, and practical steps to implementation, and then decide whether or not it can be used as a best practice.

Microglial cells are resident macrophages in the central nervous system (CNS) and become activated in many pathological conditions. Activation of microglial cells results in reactive microgliosis, manifested by an increase in cell number in the affected CNS regions. The control of microgliosis may be important to prevent pathological damage to the brain. The type 2 cytokine IL-4 has been reported to be protective in brain inflammation. However, its effect on microglial cell survival was not well understood. In this study, we report a dual effect of IL-4 on the survival of mouse microglial cells. In a 6 h short term culture, IL-4 reduced the death of microglial cells induced by staurosporine. In contrast, in long term treatment (more than 48 h), IL-4 increased the apoptotic death of both primary mouse microglial cells and a microglial cell line N9. Mechanistic studies revealed that, in microglial cells, IL-4 increased the levels of cleaved caspase 3 and PARP, which is down-stream of activated caspase 3. In addition, IL-4 down regulated the autophagy and the antiapoptotic protein Bcl-xL in microglial cells. On the other hand, the pre-incubation of microglial cells with IL-4 for 24 h, attenuated the cell death induced by the neurotoxic peptide amyloid beta 1–42 (Aβ42). Our observations demonstrate a novel function of IL-4 in regulating the survival of microglial cells, which may have important significance in reduction of undesired inflammatory responses in the CNS.

Although in recent decades, meat from hunted wild ungulates has shown interesting results in terms of market opportunities, the scientific literature is still lacking in economic studies concerning the estimation of the meat’s value for involved stakeholders. We present an analysis of the evolution of price in the local red deer meat supply chain. This analysis has been conducted through a survey based on in-depth interviews with the stakeholders involved in an Italian local supply chain. Findings derived from this study describe a case study, however, they also represent the potential dynamics of the value of Italian game meat, highlighting that the development of a sustainable local supply chain of this product may represent an economic resource for involved stakeholders.

Indicator-based tools are widely used for the assessment of farm sustainability, but analysts still face methodological and conceptual issues, including data availability, the complexity of the concept of sustainability and the heterogeneity of agricultural systems. This study contributes to this debate through the illustration of a procedure for farm sustainability assessment focussed on the case study of the South Milan Agricultural Park, Italy. The application is based on a set of environmental, social and economic indicators retrieved from the literature review. The framework is based on three main steps: (i) Data collection mainly through interviews with farmers and institutions; (ii) data elaboration through an aggregative structure; and (iii) score analysis. The latter step includes a descriptive analysis that allows a comparison among farms or groups of farms and a principal components analysis that helps to confirm the dimensions in which indicators were previously included (components). Results derived from the sampled farms show that the framework can provide easy-to-read results useful at different levels. The study highlighted the procedures for the framework construction that is compatible with the region’s context and objectives, using an analytical approach that aims at the use of balanced features of availability and reliability of data.

In the last decade, increased environmental awareness has prompted the adoption of incentives for exploiting renewable energy sources. Among these, biogas production has received a certain attention in developed countries. Nonetheless, the subsidies provided have posed the problem of an activity (the production of bioenergy) that engages in direct competition with food and feed production for limited resources, like agricultural land. Even if this competition may be softened by allocating marginal land and/or using dedicated non-agricultural crops, empirical evidence shows that biogas plants have been developed in highly-productive agricultural areas, using increasing amounts of maize silage as feedstock. Thus, studies aimed at measuring the effect of biogas production on agricultural activities are needed in order to avoid this socially undesirable outcome. The paper presents an econometric estimation of the impact of biogas plants on farmland rental values of a Northern Italian rural area. Results show that biogas has a non-linear effect on rental prices, suggesting that incentive schemes specifically accounting for plants’ dimensions and technologies would improve the social sustainability of the bioenergy sector and its coexistence with agricultural activity.

Soft-sediment deformation involves complex interactions between discrete fracturing and diffuse bulk strain, described in terms of volume change and shear strain in a critical state mechanics framework. This study reports on a mesoscale normal fault zone, intraformational in Oligocene argillaceous sediments from the Boom Formation (Belgium), containing several metre-scale normal fault strands. They form either discrete fault planes or decimetre-wide shear zones with internal fabric. The faults have been subjected to microtectonic and petrophysical analysis. Small but significant changes occur in the porous network of the argillaceous matrix approaching a fault or shear zone, indicating compactional strain in both footwall and hanging wall. Internal compaction associated with faulting is put forward as a ductile brittle feedback mechanism in the kinematics of intraformational fracture systems. Small differential stress induced by compaction and minor regional tectonic forces (differential uplift and tilt) and subsequent gravitational forces (downslope shear stress) induce small shear bands in nearly critically stressed weak mud. Shear banding is accompanied by layer-parallel shortening and bulk volume loss. This provides an additional extension of endogenous origin, accommodated by further deformation. This ductile brittle feedback mechanism eventually leads to commonly observed intraformational collapse structures called polygonal fault systems. Keywords: intraformational structures, soft-sediment deformation, argillite, strain, polygonal structures.