1. Cryopreservation of Plant Shoot Tips of Potato, Mint, Garlic, and Shallot Using Plant Vitrification Solution 3.
- Author
-
Senula A and Nagel M
- Subjects
- Cell Proliferation, Cells, Cultured, Garlic drug effects, Glycerol chemistry, Mentha drug effects, Plant Shoots drug effects, Shallots drug effects, Solanum tuberosum drug effects, Sucrose chemistry, Cell Culture Techniques methods, Cryopreservation methods, Cryoprotective Agents pharmacology, Garlic cytology, Mentha cytology, Plant Shoots cytology, Shallots cytology, Solanum tuberosum cytology
- Abstract
Cryopreservation of shoot tips facilitates long-term storage of plant genetic resources which can otherwise only be propagated vegetatively. The vitrification approach using the cryoprotectant plant vitrification solution 3 (PVS3, 50% sucrose and 50% glycerol) is easy to handle, has shown to produce high regrowth percentages in a number of potato, mint, garlic, and shallot accessions, and is, thus, highly suitable for routine cryopreservation of plant genetic resources. In the current chapter, the vitrification procedure is described for potato, mint, garlic, and shallot and includes details about modifications for the different plant species. Special emphasis is given on the preparation of the different culture media, solutions, the culture conditions prior and post-cryopreservation, and the preparation of the shoot tips from different sources. Furthermore, protocols to introduce plants into in vitro culture and methods to estimate cryopreservation success are provided.
- Published
- 2021
- Full Text
- View/download PDF