Back to Search Start Over

Micropropagation and cryopreservation of garlic (Allium sativum L.).

Authors :
Keller ER
Senula A
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2013; Vol. 11013, pp. 353-68.
Publication Year :
2013

Abstract

Garlic (Allium sativum L.) is a very important medicinal and spice plant. It is conventionally propagated by daughter bulbs ("cloves") and bulbils from the flower head. Micropropagation is used for speeding up the vegetative propagation mainly using the advantage to produce higher numbers of healthy plants free of viruses, which have higher yield than infected material. Using primary explants from bulbs and/or bulbils (shoot tips) or unripe inflorescence bases, in vitro cultures are initiated on MS-based media containing auxins, e.g., naphthalene acetic acid, and cytokinins, e.g., 6-γ-γ-(dimethylallylaminopurine) (2iP). Rooting is accompanying leaf formation. It does not need special culture phases. The main micropropagation methods rely on growth of already formed meristems. Long-term storage of micropropagated material, cryopreservation, is well-developed to maintain germplasm. The main method is vitrification using the cryoprotectant mixture PVS3.

Details

Language :
English
ISSN :
1940-6029
Volume :
11013
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
23179713
Full Text :
https://doi.org/10.1007/978-1-62703-074-8_28