1. INFLUENCE OF ACTIVATED CHARCOAL ON THE ABSORPTION OF FERROUS SULFATE IN RATS
- Author
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Gades, NM, Chyka, PA, Virgous, CK, Butler, AY, and Mandrell, TD
- Subjects
Carbon, Activated -- Health aspects ,Antidotes -- Physiological aspects ,Environmental issues ,Health ,Pharmaceuticals and cosmetics industries - Abstract
Objective: To determine whether activated charcoal alters the gastrointestinal absorption of iron as ferrous sulfate. Methods: Male Sprague-Dawley rats (236.0 [+ or -] 8.6 g; N = 75) were randomly assigned to one of five groups: 1) control given only distilled water, 2) 400 mg/kg elemental iron (approximately one-half of [LD.sub.50]) and water, 3) 1:1 ratio of charcoal to iron, 4) 2:1 ratio of charcoal to iron, and 5) 4:1 ratio of charcoal to iron. All treatments were administered consecutively by gavage within 5 minutes. Physiological measurements and blood samples were taken at 0, 1, 4, and 8 hours after treatment. Necropsies were performed at the end of the study period. Data were analyzed with ANOVA with an alpha of 0.05. Results: A total of 53 rats completed the study with the others excluded due to technical complications or premature death. There were no significant differences in physiological parameters among the 5 groups. There were no significant differences in mean (+SEM) serum iron concentrations ([micro]g/dL) among groups 2, 3, 4, and 5 except at 1 hour between groups 4 and 5 (*p [is less than] 0.004). Group N Baseline 1 hour 1 13 243 [+ or -] 84 292 [+ or -] 84 2 14 224 [+ or -] 81 1010 [+ or -] 91 3 11 215 [+ or -] 91 908 [+ or -] 91 4 7 265 [+ or -] 114 737 [+ or -] 135(*) 5 8 211 [+ or -] 107 1251 [+ or -] 114(*) Group 4 hours 8 hours 1 364 [+ or -] 84 169 [+ or -] 84 2 855 [+ or -] 87 536 [+ or -] 87 3 847 [+ or -] 107 440 [+ or -] 91 4 717 [+ or -] 135 443 [+ or -] 114 5 963 [+ or -] 107 450 [+ or -] 114 Conclusion: Activated charcoal did not alter the extent of iron absorption in the experimental model. Gades NM, Chyka PA, Virgous CK, Butler AY, Mandrell TD. University of Tennessee and Southern Poison Center, Memphis, TN, Gades NM, Chyka PA, Virgous CK, Butler AY, Mandrell TD. University of Tennessee and Southern Poison Center, Memphis, [...]
- Published
- 2000