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Your search keyword '"Gabriela T, Silveira"' showing total 4 results
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4 results on '"Gabriela T, Silveira"'

2. DMH-CBD, a cannabidiol analog with reduced cytotoxicity, inhibits TNF production by targeting NF-kB activity dependent on A2A receptor

Author

Fernando Q. Cunha, Rangel L. Silva, Thiago M. Cunha, Lucas Miranda Marques, José Alexandre de Souza Crippa, José C. Alves-Filho, Gabriela T. Silveira, Nerry T. Cecilio, Alexandre Gomes de Macedo Maganin, Marcelo Franchin, Carlos W. Wanderlei, Norberto Peporine Lopes, and Francisco Silveira Guimarães

Subjects
0301 basic medicine, Pharmacology, endocrine system, Programmed cell death, Chemistry, p38 mitogen-activated protein kinases, Antagonist, Toxicology, ANTI-INFLAMATÓRIOS, digestive system, digestive system diseases, 03 medical and health sciences, surgical procedures, operative, 030104 developmental biology, 0302 clinical medicine, 030220 oncology & carcinogenesis, medicine, Phosphorylation, Tumor necrosis factor alpha, Receptor, Cytotoxicity, Cannabidiol, medicine.drug
Abstract

Cannabidiol (CBD) is a natural compound with psychoactive therapeutic properties well described. Conversely, the immunological effects of CBD are still poorly explored. In this study, the potential anti-inflammatory effects and underlying mechanisms of CBD and its analog Dimethyl-Heptyl-Cannabidiol (DMH-CBD) were investigated using RAW 264.7 macrophages. CBD and DMH-CBD suppressed LPS-induced TNF production and NF-kB activity in a concentration-dependent manner. Both compounds reduced the NF-kB activity in a μM concentration range: CBD (IC50 = 15 μM) and DMH-CBD (IC50 = 38 μM). However, the concentrations of CBD that mediated NF-kB inhibition were similar to those that cause cytotoxicity (LC50 = 58 μM). Differently, DMH-CBD inhibited the NF-kB activation without cytotoxic effects at the same concentrations, although it provokes cytotoxicity at long-term exposure. The inhibitory action of the DMH-CBD on NF-kB activity was not related to the reduction in IkBα degradation or either p65 (NF-kB) translocation to the nucleus, although it decreased p38 MAP kinase phosphorylation. Additionally, 8-(3-Chlorostyryl) caffeine (CSC), an A2A antagonist, reversed the effect of DMH-CBD on NF-kB activity in a concentration-dependent manner. Collectively, our results demonstrated that CBD reduces NF-kB activity at concentrations intimately associated with those that cause cell death, whereas DMH-CBD decreases NF-kB activity at non-toxic concentrations in an A2A receptor dependent-manner.

Published
2019

3. Complete endoscopic resection of low-grade nasopharyngeal papillary adenocarcinoma: a case report

Author

Maristella B. Francisco dos Reis, Gabriela T. Silveira, Francesca M. Faria, Fabiana Cardoso Pereira Valera, Letícia Fontanini, Inaê M. Compagnoni, and Lorena A. Lamounier

Subjects
medicine.medical_specialty, Otorhinolaryngology, RF1-547, business.industry, Nasopharyngeal Papillary Adenocarcinoma, medicine, Endoscopic resection, Radiology, business, NASOFARINGE
Published
2021

4. DMH-CBD, a cannabidiol analog with reduced cytotoxicity, inhibits TNF production by targeting NF-kB activity dependent on A

Author

Rangel L, Silva, Gabriela T, Silveira, Carlos W, Wanderlei, Nerry T, Cecilio, Alexandre G M, Maganin, Marcelo, Franchin, Lucas M M, Marques, Norberto P, Lopes, José A, Crippa, Francisco S, Guimarães, José C F, Alves-Filho, Fernando Q, Cunha, and Thiago M, Cunha

Subjects
Secretory Pathway, Adenosine A2 Receptor Agonists, Dose-Response Relationship, Drug, Receptor, Adenosine A2A, Cell Survival, THP-1 Cells, Tumor Necrosis Factor-alpha, Macrophages, NF-kappa B, p38 Mitogen-Activated Protein Kinases, Inhibitory Concentration 50, Mice, RAW 264.7 Cells, Animals, Cannabidiol, Humans, Phosphorylation, Signal Transduction
Abstract

Cannabidiol (CBD) is a natural compound with psychoactive therapeutic properties well described. Conversely, the immunological effects of CBD are still poorly explored. In this study, the potential anti-inflammatory effects and underlying mechanisms of CBD and its analog Dimethyl-Heptyl-Cannabidiol (DMH-CBD) were investigated using RAW 264.7 macrophages. CBD and DMH-CBD suppressed LPS-induced TNF production and NF-kB activity in a concentration-dependent manner. Both compounds reduced the NF-kB activity in a μM concentration range: CBD (IC

Published
2018

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