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Захворюваність на гастроезофагеальну рефлюксну хворобу серед дітей неухильно зростає. Різноманітність клініко-морфологічних варіантів ГЕРХ зумовлює труднощі діагностики даної патології у дитячому віці. Враховуючи велику кількість етіологічних чинників, в тому числі порушення регуляції стравоходу з боку автономної нервової системи та генетичну схильність, необхідним є детальний аналіз кожного з них, Aim. To establish features of activity of different links of the autonomous nervous system in children with gastroesophageal reflux disease and different genotype of C825T locus of GNB3 gene.Materials and methods. There was realized the analysis of clinical features of GERD and functioning of the autonomous nervous system in 100 schoolchildren. The molecular-genetic study of C825T locus of GNB3 gene using PCR (rs5443) was carried out in the studied group (100 children) and in 40 healthy children, who formed the control group.Results. Sympathectomy was observed in 47 children (47%), vagotonia in 43 (43%), and euhonia was observed in 10 children (10%). Most patients with sympathectomy demonstrated normo- and hypacidity. Practically all patients with vagotonia demonstrated hyperacidity of the stomach contents.Among patients with hyperacidity (pH 0,86-1,59) the one third had СС genotype of C825T locus of GNB3 gene and persons with ТТ genotype were absent, whereas in the group of children with normo- and hypoacidity (pH 1,60-3,59)in 25% of patients was revealed ТТ genotype.Patients with СС genotype of C825T locus of GNB3 gene demonstrated vagotonia essentially more often (67,44%), and ТТ genotype was most often revealed in patients with eutonia (10%), (р0,05).2). Hyperacidity in children with GERD is associated with vagotonia.3). СС genotype of C825T locus of GNB3 gene is associated with vagotonia and hyperacidity, (р

Background/Aims A link between G protein β3 (GNB3) polymorphism and functional dyspepsia (FD) has been suggested. The aim of this study was to determine the role of GNB3 polymorphism in the long-term prognosis of FD in Koreans. Methods FD patients and normal healthy controls were recruited from patients who visited our center between December 2006 and June 2007. All of the subjects completed Rome III questionnaires before undergoing upper gastrointestinal endoscopy and colonoscopy. Genomic DNA was extracted for GNB3 genotyping. After 5 years, the subjects were reevaluated using the same questionnaires. Results GNB3 825T carrier status was significantly related to FD in Koreans (p=0.04). After 5 years, 61.0% of the initial FD patients and 12.2% of the initial normal subjects were diagnosed with FD (odds ratio [OR], 11.7; 95% confidence interval [CI], 4.3 to 31.1; p

The aim of the study was to verify the hypothesis if the interaction between the G protein beta3 subunit (GNB3) C825T polymorphism and ACE I/D polymorphism could lead to the disclosure of increased activity of sodium-proton exchanger and hypertension.The study included 44 male patients, median age: 40 years. Patients were divided into two groups: 26 patients with essential hypertension (EH), and 18 subjects in the normotensive group (C).CT + TT genotypes of GNB3 predominated in patients with hypertension (65%) compared to normotensive patients (12%) (p0.01). No significant differences were observed in the frequency of ACE gene polymorphisms between the examined groups. Significantly higher activity of erythrocyte NHE in patients with EH was observed: median 8.83 (interquartile range 4.27) mmol/l RBC/h, compared to C: median 6.18 (2.80) mmol/l RBC/h, p0.001. Multiple logistic regression analysis showed that the presence of the T allele increased the risk of hypertension 16-fold (p0.01) and higher erythrocyte NHE activity 2-fold per each unit of activity (p0.01). DD genotype of ACE polymorphism did not increase the risk of hypertension. No significant interaction of the influence of GNB3 T allele and ACE DD genotype on the risk of hypertension was observed. In multiple linear regression analysis, none of the examined genotypes and their interactions influenced NHE activity.The presence of the T allele of GNB3 polymorphism and increased activity of erythrocyte NHE independently of ACE genotype increase the risk of hypertension.