1. Synergism of interleukin-12 and interleukin-3 on development of hematopoietic progenitors
- Author
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M. Dzondo‐Gadet, J. P. Vannier, G. Teixeira‐Lebrun, B. Lenormand, and D. Fardoun‐Joalland
- Subjects
Myeloid ,medicine.medical_treatment ,Cell ,Biology ,medicine ,Humans ,Progenitor cell ,Interleukin 3 ,Erythroid Precursor Cells ,Dose-Response Relationship, Drug ,Interleukin-6 ,Growth factor ,Granulocyte-Macrophage Colony-Stimulating Factor ,Drug Synergism ,Hematology ,General Medicine ,Hematopoietic Stem Cells ,Interleukin-12 ,Cell biology ,Hematopoiesis ,Haematopoiesis ,medicine.anatomical_structure ,Immunology ,Interleukin 12 ,Interleukin-3 ,Stem cell - Abstract
The recently cloned cytotoxic lymphocyte maturation factor [CLMF] also called NK cell stimulatory factor [NKSF] or interleukin-12 [IL-12] has been described as a growth factor for mature lymphoid cells. The present study investigated whether purified recombinant human IL-12 could stimulate CFU colony growth. Source of progenitor cells were peripheral blood cells depleted of adherent, CD2- and CD56-positive cells. RhIL-12 was investigated either alone or in combination with rhIL-3, rhIL-6 and rhGM-CSF. RhIL-12 alone did not support colony formation of myeloid or erythroid progenitors. RhIL-12 in combination with rhIL-3 increased the numbers of BFU-E and CFU-GM. No synergism or additive effect was seen with the combination of rhIL-12 and rhGM-CSF or rhIL-12 and rhIL-6. An additive increase in the number of granulocytic colonies was observed when rhIL-3, rhIL-6 and rhGM-CSF were used together with rhIL-12. Our result therefore suggest that, in addition to being a potent lymphopoietic stimulator, IL-12 acts synergistically with IL-3 in enhancing the sensitivity of hemopoietic progenitors to IL-3.
- Published
- 1995