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Synergism of interleukin-12 and interleukin-3 on development of hematopoietic progenitors
- Source :
- European journal of haematology. 54(3)
- Publication Year :
- 1995
-
Abstract
- The recently cloned cytotoxic lymphocyte maturation factor [CLMF] also called NK cell stimulatory factor [NKSF] or interleukin-12 [IL-12] has been described as a growth factor for mature lymphoid cells. The present study investigated whether purified recombinant human IL-12 could stimulate CFU colony growth. Source of progenitor cells were peripheral blood cells depleted of adherent, CD2- and CD56-positive cells. RhIL-12 was investigated either alone or in combination with rhIL-3, rhIL-6 and rhGM-CSF. RhIL-12 alone did not support colony formation of myeloid or erythroid progenitors. RhIL-12 in combination with rhIL-3 increased the numbers of BFU-E and CFU-GM. No synergism or additive effect was seen with the combination of rhIL-12 and rhGM-CSF or rhIL-12 and rhIL-6. An additive increase in the number of granulocytic colonies was observed when rhIL-3, rhIL-6 and rhGM-CSF were used together with rhIL-12. Our result therefore suggest that, in addition to being a potent lymphopoietic stimulator, IL-12 acts synergistically with IL-3 in enhancing the sensitivity of hemopoietic progenitors to IL-3.
- Subjects :
- Myeloid
medicine.medical_treatment
Cell
Biology
medicine
Humans
Progenitor cell
Interleukin 3
Erythroid Precursor Cells
Dose-Response Relationship, Drug
Interleukin-6
Growth factor
Granulocyte-Macrophage Colony-Stimulating Factor
Drug Synergism
Hematology
General Medicine
Hematopoietic Stem Cells
Interleukin-12
Cell biology
Hematopoiesis
Haematopoiesis
medicine.anatomical_structure
Immunology
Interleukin 12
Interleukin-3
Stem cell
Subjects
Details
- ISSN :
- 09024441
- Volume :
- 54
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- European journal of haematology
- Accession number :
- edsair.doi.dedup.....b029dbfce0df9d737e23e6aa0422aafb