244 results on '"G, Chiappetta"'
Search Results
2. Peer Review #1 of 'Heat shock protein 90 is involved in the regulation of HMGA2-driven growth and epithelial-to-mesenchymal transition of colorectal cancer cells (v0.1)'
- Author
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G Chiappetta
- Published
- 2016
3. Catalytic membrane reactors for tritium recovery from tritiated water in the ITER fuel cycle
- Author
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Angelo Basile, V. Violante, Silvano Tosti, S Castelli, Francesca Sarto, S Scaglione, G Chiappetta, and M. De Francesco
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Materials science ,Hydrogen ,Membrane reactor ,Mechanical Engineering ,chemistry.chemical_element ,engineering.material ,Water-gas shift reaction ,Catalysis ,Membrane ,Nuclear Energy and Engineering ,Chemical engineering ,chemistry ,Coating ,engineering ,General Materials Science ,Thin film ,Civil and Structural Engineering ,Palladium - Abstract
Palladium and palladium–silver permeators have been obtained by coating porous ceramic tubes with a thin metal layer. Three coating techniques have been studied and characterized: chemical electroless deposition (PdAg film thickness of 10 μm), ion sputtering (about 1 μm) and rolling of thin metal sheets (50 μm). The Pd-ceramic membranes have been used for manufacturing catalytic membrane reactors (CMR) for hydrogen and its isotopes recovering and purifying. These composite membranes and the CMR have been studied and developed for a closed-loop process with reference to the design requirements of the international thermonuclear experimental reactor (ITER) blanket tritium recovery system in the enhanced performance phase of operation. The membranes and CMR have been tested in a pilot plant equipped with temperature, pressure and flow-rate on-line measuring and controlling devices. The conversion value for the water gas shift reaction in the CMR has been measured close to 100% (always above the equilibrium one, 80% at 350°C): the effect of the membrane is very clear since the reaction is moved towards the products because of the continuous hydrogen separation. The rolled thin film membranes have separated the hydrogen from other gases with a complete selectivity and exhibited a slightly larger mass transfer resistance with respect to the electroless membranes. Preliminary tests on the sputtered membranes have also been carried out with a promising performance. Considerations on the use of different palladium alloy in order to improve the performances of the membranes in terms of permeation flux and mechanical strength, such as palladium/yttrium, are also reported.
- Published
- 2000
4. An approach to whole blood serum proteomics using flow field-flow fractionation with multiangle laser scattering detection and nanochip-ion trap mass spectrometry
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RAMBALDI, DIANA CRISTINA, ZATTONI, ANDREA, RESCHIGLIAN, PIERLUIGI, A. Amoresano, G. Chiappetta, G. Marino, D.C. Rambaldi, A. Zattoni, P. Reschiglian, A. Amoresano, G. Chiappetta, and G. Marino
- Published
- 2007
5. Gene rearrangement and Chernobyl related thyroid cancers
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Gisela Keller, N D Tronko, T Bogdanova, V Pozcharskaya, G. A. Thomas, E P Demidchik, Giancarlo Vecchio, M Tonnachera, L Voscoboinik, Alfredo Fusco, Heinz Höfler, G Chiappetta, A Carss, E D Williams, H. Bunnell, Jacques Emile Dumont, J Parma, Massimo Santoro, Gareth H. Williams, E. D. Cherstvoy, Santoro, Massimo, G. A., Thoma, Vecchio, Giancarlo, G. H., William, Fusco, Alfredo, G., Chiappetta, V., Pozcharskaya, T. I., Bogdanova, E. P., Demidchik, E. D., Cherstvoy, L., Voscoboinik, N. D., Tronko, A., Car, H., Bunnell, M., Tonnachera, J., Parma, J. E., Dumont, G., Keller, H., Höfler, and E. D., Williams
- Subjects
Male ,Cancer Research ,Pathology ,etiology/genetics, Ukraine ,Neoplasms, Radiation-Induced ,Neoplasms, Radiation-Induced -- genetics ,oncogenes ,Chromosomes, Human, Pair 10 -- genetics ,Proto-Oncogenes -- genetics ,Papillary ,medicine.disease_cause ,ref ,Disasters ,Exon ,thyroid cancer ,Carcinoma, Papillary -- etiology ,Pair 10 ,genetics, Power Plants, Proto-Oncogene ,Child ,Thyroid cancer ,Thyroid ,Adolescent, Carcinoma ,Regular Article ,Sciences bio-médicales et agricoles ,Newborn, Male, Neoplasm ,medicine.anatomical_structure ,Oncology ,Child, Preschool ,Female ,Radioactive Hazard Release ,Ukraine ,Human ,medicine.medical_specialty ,endocrine system ,Tumor suppressor gene ,Adolescent ,etiology/genetics, Child, Child ,Preschool, Chromosome ,gene rearrangement ,Biology ,Chernobyl ,Thyroid carcinoma ,Proto-Oncogenes ,Carcinoma ,medicine ,Humans ,Thyroid Neoplasms ,genetics, Disasters, Female, Humans, Infant, Infant ,Thyroid Neoplasms -- genetics ,Chromosomes, Human, Pair 10 ,Carcinoma, Papillary -- genetics ,Infant, Newborn ,Infant ,Gene rearrangement ,Thyroid Neoplasms -- etiology ,medicine.disease ,Carcinoma, Papillary ,Radiation-Induced ,genetics, Radioactive Hazard Release, Thyroid Neoplasm ,Carcinogenesis ,Power Plants - Abstract
The increase in thyroid carcinoma post-Chernobyl has been largely confined to a specific subtype of papillary carcinoma (solid/follicular). This subtype is observed predominantly in children under 10 in unirradiated populations, but maintains a high frequency in those aged 10-15 from those areas exposed to fallout from the Chernobyl accident. The aim of this study was to link morphology with molecular biology. We examined 106 papillary carcinomas from children under the age of 15 at operation. All were examined for rearrangements of the RET oncogene by reverse transcription polymerase chain reaction (RT-PCR); a subset of these cases were also examined for mutations of the three ras oncogenes, exon 10 of the thyroid stimulating hormone receptor, associated more usually with a follicular rather than papillary morphology, and exons 5, 6, 7 and 8 of the p53 gene, commonly involved in undifferentiated thyroid carcinoma. Rearrangements of the REToncogene were found in 44% of papillary carcinomas in which we studied fresh material; none of the tumours examined showed mutation in any of the other genes. The two rearrangements resulting from inversion of part of chromosome 10 (PTC1 and PTC3) accounted for the majority of RET rearrangements identified, with PTC1 being associated with papillary carcinomas of the classic and diffuse sclerosing variants and PTC3 with the solid/follicular variant., Journal Article, Research Support, Non-U.S. Gov't, info:eu-repo/semantics/published
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- 2000
6. Involvement of the HMGI(Y) gene in a microfollicular adenoma of the thyroid
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P. D. Cin, G. Belge, G. Chiappetta, M. Fedele, P. Pauwels, R. Bullerdiek, H. V. den, FUSCO, ALFREDO, P. D., Cin, Fusco, Alfredo, G., Belge, G., Chiappetta, M., Fedele, P., Pauwel, R., Bullerdiek, and H. V., Den
- Abstract
Follicular thyroid adenomas are epithelial tumors characterized by subgroups with specific chromosomal aberrations. Here we present a case with translocation t(1;6)(p35;p21). In 1p35 and 6p21, two genes of the high- mobility group of proteins are located. With P1-derived Artificial Chromosome (PACs) derived from the HMG17 gene located at 1p35 and HMG1(Y) located at 6p21, fluorescence in situ hybridization was performed. The breakpoints were located distal to HMG17 and proximal to HMG1(Y), but no rearrangement of the genes was shown by FISH. However, an overexpression of the HMG1(Y) gene was detected by RT-PCR as well as by immunohistochemistry techniques. This suggests a breakpoint in the proximity of the HMG1(Y) deregulating HMG1(Y) gene expression, a situation found in a variety of human benign mesenchymal tumors with involvement of chromosome band 6p21.
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- 1999
7. Membrane Reactors on small scale for the methane steam reforming reaction: assessment on the high pressure importance
- Author
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G. Chiappetta, G. Barbieri, and E. Drioli
- Published
- 2009
8. The Expression of the High-mobility Group Hmgi (y) Proteins Correlates With the Malignant Phenotype of Human Thyroid Neoplasias
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G. CHIAPPETTA, A. BANDIERA, M. T. BERLINGIERI, R. VISCONTI, G. MANFIOLETTI, S. BATTISTA, F. J. MARTINEZTELLO, V. GIANCOTTI, SANTORO, MASSIMO, FUSCO, ALFREDO, G., Chiappetta, A., Bandiera, M. T., Berlingieri, R., Visconti, G., Manfioletti, S., Battista, F. J., Martineztello, Santoro, Massimo, V., Giancotti, and Fusco, Alfredo
- Abstract
High Mobility Group I(HMGI) proteins are nuclear proteins involved in the regulation of chromatin structure and function, Elevated expression of the HMGI proteins (HMGI, HMGY and HMGI-C) has been correlated with the presence of a highly malignant phenotype in epithelial and fibroblastic rat thyroid cells, and in several experimental carcinomas, Here, we demonstrate that HMGI and HMGY proteins are expressed in human thyroid carcinomas and thyroid carcinoma cell lines, but not in adenomas, goiters, normal thyroid tissues and cells, These results indicate a correlation between HMGI and HMGY expression and the malignant phenotype of thyroid neoplasias, suggesting that these proteins may be used as markers in thyroid cancer.
- Published
- 1995
9. THE RET PROTOONCOGENE IS EXPRESSED IN PREDOMINANTLY EPITHELIAL HUMAN THYMOMAS
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M. Santoro, G. Botti, P. M. Dubois, C. Paulin, N. Fabien, M. Grieco, G. Chiappetta, A. Fusco, N., Fabien, C., Paulin, Santoro, Massimo, G., Chiappetta, G., Botti, M., Grieco, P. M., Duboi, and Fusco, Alfredo
- Subjects
Cancer Research ,Thymoma ,Oncogene ,Cell ,Cancer ,In situ hybridization ,Cell cycle ,RET proto-oncogene ,Biology ,medicine.disease ,Molecular medicine ,medicine.anatomical_structure ,Oncology ,hemic and lymphatic diseases ,medicine ,Cancer research ,neoplasms - Abstract
Thymomas are thymic tumors composed of epithelial cells with various number of lymphocytes. On the basis of lymphocytes number, they can be histologically classified into predominantly lymphocytic, predominantly epithelial and mixed lympho-epithelial. These tumors are rare and their prognosis is not well known yet. In this study we demonstrate by in situ hybridization the presence of RET transcripts in the medullary part of normal murine thymus and in epithelial thymomas. Conversely RET expression was not detected in the cortical part of the thymus and in thymomas with the predominance of the lymphocytic component, suggesting that its expression could be a specific marker of the predominantly epithelial histotype.
- Published
- 1994
10. Preliminary analysis on the potentiality of a palladium-based membrane reactor for producing high purity hydrogen to be used in PEM fuel cells
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E. Drioli, A. Criscuoli, G. Clarizia, and G. Chiappetta
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- 2003
11. High Mobility Group (HMGA1a) protein expression as a diagnostic indicator of human colorectal neoplastic diseases
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G. Chiappetta, G. Manfioletti, F. Pentimalli, M. Molnaco, R. Pasquinelli, T. Watanabe, M. Fedele, G. Viglietto, M. Santoro, and V. Giancotti A. Fusco
- Published
- 2002
12. Retraction Note: High-mobility group A1 protein inhibits p53-mediated intrinsic apoptosis by interacting with Bcl-2 at mitochondria
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Antonio Federico, Mara Tornincasa, Giovanna Maria Pierantoni, Franca Esposito, G Chiappetta, and Alfredo Fusco
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Cancer Research ,Transcription, Genetic ,Ultraviolet Rays ,Immunology ,Apoptosis ,macromolecular substances ,Mitochondrion ,Bioinformatics ,Cell Line ,Cellular and Molecular Neuroscience ,Humans ,Medicine ,HMGA1a Protein ,business.industry ,digestive, oral, and skin physiology ,Intrinsic apoptosis ,Cytochromes c ,Cell Biology ,Recombinant Proteins ,humanities ,Retraction ,Mitochondria ,Cell biology ,HEK293 Cells ,High-mobility group ,Proto-Oncogene Proteins c-bcl-2 ,Caspases ,MCF-7 Cells ,Tumor Suppressor Protein p53 ,business ,HeLa Cells ,Protein Binding - Abstract
The high-mobility group A (HMGA) proteins are a family of non-histone chromatin factors, encoded by the HMGA1 and HMGA2 genes. Several studies demonstrate that HMGA proteins have a critical role in neoplastic transformation, and their overexpression is mainly associated with a highly malignant phenotype, also representing a poor prognostic index. Even though a cytoplasmic localization of these proteins has been previously reported in some highly malignant neoplasias, a clear role for this localization has not been defined. Here, we first confirm the localization of the HMGA1 proteins in the cytoplasm of cancer cells, and then we report a novel mechanism through which HMGA1 inhibits p53-mitochondrial apoptosis by counteracting the binding of p53 to the anti-apoptotic factor Bcl-2. Indeed, we demonstrate a physical and functional interaction between HMGA1 and Bcl-2 proteins. This interaction occurs at mitochondria interfering with the ability of p53 protein to bind Bcl-2, thus counteracting p53-mediated mitochondrial apoptosis. This effect is associated with the inhibition of cytochrome c release and activation of caspases. Consistent with this mechanism, a strong correlation between HMGA1 cytoplasmic localization and a more aggressive histotype of thyroid, breast and colon carcinomas has been observed. Therefore, cytoplasmic localization of HMGA1 proteins in malignant tissues is a novel mechanism of inactivation of p53 apoptotic function.
- Published
- 2014
13. Pancreatic duct cell carcinomas express high levels of high mobility group I(Y) proteins
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N, Abe, T, Watanabe, T, Masaki, T, Mori, M, Sugiyama, H, Uchimura, Y, Fujioka, G, Chiappetta, A, Fusco, and Y, Atomi
- Subjects
Pancreatic Neoplasms ,Phenotype ,Pancreatitis ,Papilloma ,Carcinoma ,Cystadenoma, Serous ,High Mobility Group Proteins ,Pancreatic Ducts ,Humans ,HMGA1a Protein ,Immunohistochemistry ,Transcription Factors - Abstract
The high mobility group I (HMGI) family of proteins in mammals belongs to a group of nonhistone nuclear proteins known as architectural transcriptional factors. They function in vivo as both structural components of chromatin and auxiliary gene transcription factors. In an earlier study (N. Abe et al, Cancer Res., 59: 1169-1174, 1999), we demonstrated that the expression level of the HMGI(Y) gene/proteins was significantly increased in colorectal adenocarcinoma and colorectal adenoma with severe cellular atypia. In the current study, we analyzed HMGI(Y) expression in several human pancreatic lesions to investigate (a) whether HMGI(Y) overexpression is also observed in pancreatic carcinoma, and (b) the role of HMGI(Y) in the diagnosis of pancreatic neoplasms. To this end, HMGI(Y) expression was determined at the protein level by immunohistochemistry using a HMGI(Y)-specific antibody in 6 surgically resected specimens of nonneoplastic tissue (4 specimens of normal pancreatic tissue and 2 specimens of chronic pancreatitis tissue), 8 pancreatic cystic neoplasms (5 intraductal papillary mucinous adenomas, 1 serous cystadenoma, and 2 solid pseudopapillary tumors), and 15 duct cell carcinomas of the pancreas. Immunohistochemical analysis revealed intense nuclear staining in the pancreatic carcinoma cells, whereas only very faint nuclear staining was seen in the nonneoplastic cells. There was a strong correlation between HMGI(Y) protein overexpression and a diagnosis of carcinoma (P = 0.000018). Thus, an increased expression level of the HMGI(Y) proteins was clearly associated with the malignant phenotype in pancreatic tissue. In addition, a low level of protein expression was also apparent in two of the cystic neoplasms that exhibited cellular atypia, but not in those that did not exhibit cellular atypia. Based on these findings, we propose that the HMGI(Y) proteins could be closely associated with tumorigenesis in the pancreas and that HMGI(Y) could serve as a potential diagnostic molecular marker for distinguishing pancreatic malignancies unambiguously from normal tissue or benign lesions.
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- 2000
14. Involvement of the HMGI(Y) gene in a microfollicular adenoma of the thyroid
- Author
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P, Dal Cin, A, Fusco, G, Belge, G, Chiappetta, M, Fedele, P, Pauwels, J, Bullerdiek, and H, Van den Berghe
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Adenoma ,Adult ,Male ,Reverse Transcriptase Polymerase Chain Reaction ,High Mobility Group Proteins ,Immunohistochemistry ,Translocation, Genetic ,Chromosome Banding ,Neoplasm Proteins ,Humans ,HMGA1a Protein ,Thyroid Neoplasms ,In Situ Hybridization, Fluorescence ,Transcription Factors - Abstract
Follicular thyroid adenomas are epithelial tumors characterized by subgroups with specific chromosomal aberrations. Here we present a case with translocation t(1;6)(p35;p21). In 1p35 and 6p21, two genes of the high-mobility group of proteins are located. With P1-derived Artificial Chromosome (PACs) derived from the HMG17 gene located at 1p35 and HMGI(Y) located at 6p21, fluorescence in situ hybridization was performed. The breakpoints were located distal to HMG17 and proximal to HMGI(Y), but no rearrangement of the genes was shown by FISH. However, an overexpression of the HMGI(Y) gene was detected by RT-PCR as well as by immunohistochemistry techniques. This suggests a breakpoint in the proximity of the HMGI(Y) deregulating HMGI(Y) gene expression, a situation found in a variety of human benign mesenchymal tumors with involvement of chromosome band 6p21.
- Published
- 1999
15. Determination of high mobility group I(Y) expression level in colorectal neoplasias: a potential diagnostic marker
- Author
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N, Abe, T, Watanabe, M, Sugiyama, H, Uchimura, G, Chiappetta, A, Fusco, and Y, Atomi
- Subjects
Adult ,Aged, 80 and over ,Male ,High Mobility Group Proteins ,Middle Aged ,Prognosis ,Cell Transformation, Neoplastic ,Lymphatic Metastasis ,Biomarkers, Tumor ,Humans ,Female ,Neoplasm Invasiveness ,HMGA1a Protein ,RNA, Messenger ,Colorectal Neoplasms ,Aged ,Transcription Factors - Abstract
High mobility group I(Y) [HMGI(Y)] proteins are architectural factors abundantly expressed during embryogenesis, and their overexpression is known to be closely associated with neoplastic transformation of cells. This study was performed to investigate whether determination of HMGI(Y) expression level could assist in (a) differential diagnosis between colorectal carcinoma, adenoma, and normal tissue and (b) determination of the prognosis of patients with colorectal cancer. To this end, HMGI(Y) expression was determined at both the protein and mRNA levels in 30 colorectal carcinomas, 26 adenomas, and 23 normal mucosa samples, and further correlations between the protein expression levels and various clinicopathological parameters, such as depth of tumor invasion, lymphatic and/or venous involvement, regional lymph node metastasis, and Dukes' stage, were determined in 30 carcinoma cases. The expression of HMGI(Y) proteins was significantly increased in carcinoma and adenoma with severe atypia compared with that in adenoma with less atypia and normal colorectal mucosa. This increase in HMGI(Y) protein expression was found to be because of an increase in its mRNA expression by RNA in situ hybridization analysis. Clinicopathological analysis revealed that the level of HMGI(Y) protein expression was significantly correlated with parameters known to be indicative of a poor prognosis in colorectal cancer patients. These findings indicate that the determination of the HMGI(Y) protein expression level could be a potential marker for the diagnosis of colorectal neoplasias and can be of great value in predicting the prognosis of patients with colorectal cancer.
- Published
- 1999
16. RET/PTC oncogene activation defines a subset of papillary thyroid carcinomas lacking evidence of progression to poorly differentiated or undifferentiated tumor phenotypes
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G, Tallini, M, Santoro, M, Helie, F, Carlomagno, G, Salvatore, G, Chiappetta, M L, Carcangiu, and A, Fusco
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Adult ,Gene Rearrangement ,Male ,Adolescent ,Proto-Oncogene Proteins c-ret ,Age Factors ,Infant ,Receptor Protein-Tyrosine Kinases ,Cell Differentiation ,Immunohistochemistry ,Proto-Oncogene Mas ,Carcinoma, Papillary ,Gene Expression Regulation, Neoplastic ,Phenotype ,Isomerism ,Risk Factors ,Child, Preschool ,Proto-Oncogene Proteins ,Disease Progression ,Drosophila Proteins ,Humans ,Female ,Thyroid Neoplasms ,Child - Abstract
Malignant tumors of the thyroid gland vary considerably in aggressiveness, ranging from a well-differentiated, clinically indolent, to an undifferentiated, often lethal phenotype. Undifferentiated (anaplastic) thyroid tumors are supposed to be derived, through a process of progression, from previously differentiated neoplasms. A common genetic alteration in thyroid tumors is the rearrangement of the tyrosine kinase-encoding RET proto-oncogene, leading to the generation of chimeric RET/PTC oncogenes. To define the characteristics of the thyroid tumor subset with RET rearrangements, we have investigated its activation by a combined immunohistochemistry and reverse transcription-PCR approach in a series of 316 well-characterized thyroid tumors representative of the main diagnostic groups. RET activation was detected in 81 of 201 (40.3%) papillary carcinomas. It correlated with tumors exhibiting the "classic" morphological features of papillary cancer or with the microcarcinoma subtype (P = 0.017). RET activation in papillary carcinoma was not associated with clinical markers (such as large tumor size, extrathyroidal extension, or metastases) of increased morbidity. Follicular-type neoplasms (61 adenomas and 22 carcinomas), as well as the aggressive poorly differentiated (15 cases) or undifferentiated (anaplastic) carcinomas (17 cases), were negative. This study demonstrates that all thyroid carcinomas harboring activating RET rearrangements exhibit a well-differentiated phenotype, that of papillary carcinoma, and indicates that the subset of RET/PTC-positive papillary carcinomas do not progress to more aggressive, less differentiated tumor phenotypes.
- Published
- 1998
17. Increase in AP-1 activity is a general event in thyroid cell transformation in vitro and in vivo
- Author
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S, Battista, F, de Nigris, M, Fedele, G, Chiappetta, S, Scala, D, Vallone, G M, Pierantoni, T, Mega, M, Santoro, G, Viglietto, P, Verde, A, Fusco, T, Megar, Battista, S, de NIGRIS, Filomena, Fedele, M, Chiappetta, G, Scala, S, Vallone, D, Pierantoni, Gm, Mega, T, Santoro, M, Viglietto, G, Verde, P, Fusco, A., Battista, S., de Nigris, F., Fedele, M., Chiappetta, G., Scala, S., Vallone, D., Pierantoni, GIOVANNA MARIA, Mega, T., Santoro, Massimo, Viglietto, G., Verde, P., and Fusco, Alfredo
- Subjects
endocrine system ,Cancer Research ,medicine.medical_specialty ,endocrine system diseases ,JUNB ,Proto-Oncogene Proteins c-jun ,Thyroid Gland ,Thyrotropin ,Biology ,Polymerase Chain Reaction ,Thyroglobulin ,Thyroid carcinoma ,Genes, jun ,In vivo ,Internal medicine ,Genetics ,medicine ,Animals ,Humans ,Neoplastic transformation ,Thyroid Neoplasms ,Molecular Biology ,Oncogene ,Thyroid ,HMGA2 Protein ,High Mobility Group Proteins ,Epithelial Cells ,Oncogenes ,In vitro ,Neoplasm Proteins ,Rats ,Transcription Factor AP-1 ,Endocrinology ,medicine.anatomical_structure ,Cell Transformation, Neoplastic ,Cell culture ,Cancer research ,Proto-Oncogene Proteins c-fos - Abstract
We have recently reported that neoplastic transformation of two rat thyroid epithelial cell lines by retroviruses carrying the v-mos and v-ras Ki oncogenes is associated with a drastic increase of AP-1 activity. The most important effects were represented by the dramatic junB and fra-1 gene induction, which was abolished by the block of the transformation-induced HMGI-C protein synthesis. Here, we have further characterized the transformation-dependent AP-1 activity, by analysing the expression of different jun- and fos-related components, in rat thyroid cell lines transformed by several oncogenes, in human thyroid carcinoma cell lines, and in naturally occurring human thyroid tumours. A significant increase of Fra-1 and JunB protein levels was detected in all oncogene transformed rat thyroid cell lines. Fra-1 gene induction was demonstrated to occur also in human thyroid carcinoma cell lines and tissues. Conversely, c-Jun and JunD proteins, rather than JunB, accumulated in human thyroid carcinoma cell lines. An induction of AP-1 target genes was also detected both in rat and human thyroid transformed cell lines. Therefore, in vivo and in vitro thyroid cell transformation is associated with important compositional changes in the AP-1 complex and an increased transcriptional activity.
- Published
- 1998
18. Neovascularization in human germ cell tumors correlates with a marked increase in the expression of the vascular endothelial growth factor but not the placenta-derived growth factor
- Author
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G, Viglietto, A, Romano, D, Maglione, M, Rambaldi, I, Paoletti, C T, Lago, D, Califano, C, Monaco, A, Mineo, G, Santelli, G, Manzo, G, Botti, G, Chiappetta, and M G, Persico
- Subjects
Male ,Teratocarcinoma ,Vascular Endothelial Growth Factor A ,Lymphokines ,Base Sequence ,Neovascularization, Pathologic ,Transcription, Genetic ,Vascular Endothelial Growth Factors ,Blotting, Western ,Molecular Sequence Data ,Receptor Protein-Tyrosine Kinases ,Endothelial Growth Factors ,Pregnancy Proteins ,Polymerase Chain Reaction ,Cell Hypoxia ,Isomerism ,Testicular Neoplasms ,Carcinoma, Embryonal ,Tumor Cells, Cultured ,Humans ,Choriocarcinoma ,Germinoma ,RNA, Messenger ,Placenta Growth Factor - Abstract
Neoangiogenesis is a prerequisite for tumor growth and metastasis. In germ cell cancer patients with the disease limited to the testicle (stage A), tumor-associated neovascularization is predictive of metastatic disease (stage B). To investigate the molecular mechanisms underlying neovascularization in human germ cell tumors (GCTs), we analysed the expression of two angiogenic growth factors, vascular endothelial growth factor (VEGF) and placenta growth factor (P1GF), and of their receptors (FLT-1) and Flk-1/KDR) in a panel of testicular tumors. In this study we show a marked increase in VEGF expression in 36/44 (81.8%) primary testicular-derived GCTs, as compared to normal testis, that significantly correlates with a high density of intratumor microvessels (r = 0.72461, P0.001; n = 24). As determined by RT - PCR and/or Western blot, the predominant VEGF isoforms expressed in GCTs are the VEGF121 and VEGF165, which are more efficiently secreted by the cells, and thus more active in eliciting angiogenesis. Conversely, in the case of PIGF, only a weak correlation with the vascular density of tumors is observed (r = 0.26599, P0.05; n = 24). Northern blot analysis also revealed significant up-regulation of VEGF/ PIGF receptors in highly vascularized germ cell tumors, compared to normal testes. These findings suggest that VEGF may act in a paracrine manner to induce neovascularization, oedema extravasation and cyst formation in human germ cell tumors. The correlation between VEGF expression and the vascular density of tumors, suggest that the evaluation of VEGF expression may be of help in predicting patients at risk for metastatic diseases. Finally, we demonstrate that VEGF up-regulation may occur at the RNA level since no gene amplification is observed; conversely, in in vitro models such as the embryonal stem cell line NTERA-2 and the choricarcinoma JEG-3 cell line, VEGF (but not PIGF) mRNA expression is regulated by hypoxic stress.
- Published
- 1996
19. Development of thyroid papillary carcinomas secondary to tissue-specific expression of the RET/PTC1 oncogene in transgenic mice
- Author
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M, Santoro, G, Chiappetta, A, Cerrato, D, Salvatore, L, Zhang, G, Manzo, A, Picone, G, Portella, G, Santelli, G, Vecchio, and A, Fusco
- Subjects
Saccharomyces cerevisiae Proteins ,Base Sequence ,Molecular Sequence Data ,Proto-Oncogene Proteins c-ret ,Receptor Protein-Tyrosine Kinases ,Mice, Transgenic ,Immunohistochemistry ,Proto-Oncogene Mas ,Thyroglobulin ,Carcinoma, Papillary ,Rats ,Gene Expression Regulation, Neoplastic ,Protein Phosphatase 2C ,Mice ,Proto-Oncogene Proteins ,Phosphoprotein Phosphatases ,Animals ,Drosophila Proteins ,Protein Phosphatase 2 ,Thyroid Neoplasms ,Promoter Regions, Genetic ,Plasmids - Abstract
Gene rearrangements activating the RET proto-oncogene are frequently associated with human thyroid carcinomas belonging to the papillary subtype. These arrangements cause the fusion of the tyrosine-kinase domain of RET to the 5'-terminal region of different genes creating the RET/PTC chimeric oncogenes. Here we report the generation of transgenic mice lines expressing the RET/PTC1 oncogene under the control of the thyroid-specific rat thyroglobulin promoter. RET/PTC1-transgenic mice developed thyroid tumors displaying the histological aspect of papillary carcinomas. These tumors were slowly progressive and did not cause premature death of the animals. Two additional mice developed areas of thyroid hyperplasia. Immunohistochemical and reverse-transcriptase polymerase chain reaction analyses confirmed the thyroid-specific expression of the transgene. Given the frequency of activating rearrangements of RET in human papillary thyroid carcinomas we conclude that this animal system could be a good model for studying the neoplastic progression of thyroid carcinomas.
- Published
- 1996
20. Upregulation of vascular endothelial growth factor (VEGF) and downregulation of placenta growth factor (PlGF) associated with malignancy in human thyroid tumors and cell lines
- Author
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G, Viglietto, D, Maglione, M, Rambaldi, J, Cerutti, A, Romano, F, Trapasso, M, Fedele, P, Ippolito, G, Chiappetta, and G, Botti
- Subjects
Vascular Endothelial Growth Factor A ,Lymphokines ,Vascular Endothelial Growth Factor Receptor-1 ,Goiter ,Vascular Endothelial Growth Factors ,Blotting, Western ,Carcinoma ,Receptor Protein-Tyrosine Kinases ,Endothelial Growth Factors ,Pregnancy Proteins ,Precipitin Tests ,Up-Regulation ,Gene Expression Regulation, Neoplastic ,Immunoenzyme Techniques ,Receptors, Vascular Endothelial Growth Factor ,Proto-Oncogene Proteins ,Tumor Cells, Cultured ,Humans ,Receptors, Growth Factor ,RNA, Messenger ,RNA, Neoplasm ,Thyroid Neoplasms ,Placenta Growth Factor - Abstract
Vascular endothelial growth factor (VEGF) is a potent mitogen for endothelial cells in vitro, promotes neoangiogenesis in vivo and increases the permeability of the vascular endothelium. VEGF overexpression occurs in several cultured tumor cell lines and in certain human malignancies. Placenta growth factor (PlGF) is a recently identified growth factor for endothelial cells (EC); PlGF strongly potentiates both the proliferative and the permeabilization effects exerted by VEGF on the vascular endothelium. To uncover the molecular mechanisms underlying neoangiogenesis in human thyroid tumors, we have analysed VEGF and PlGF expression in a panel of thyroid carcinoma cell lines with different tumorigenic potential as well as in human primary thyroid tumors. We show that a high tumorigenic potential is associated with an elevated VEGF expression in human thyroid tumor cell lines. Furthermore, VEGF overexpression occurs in 5/5 highly malignant anaplastic carcinomas. Papillary and follicular carcinomas express intermediate levels of VEGF mRNA. In contrast, PlGF expression is severely down regulated in the majority of thyroid tumor cell lines and in tumors. Furthermore, we show that both the VEGF receptors, FLT-1 and flk/KDR, are expressed in endothelial cells that line tumor-embedded microvascular vessels, suggesting that VEGF but not PlGF, contributes to thyroid tumor development.
- Published
- 1995
21. High-mobility group A1 protein inhibits p53-mediated intrinsic apoptosis by interacting with Bcl-2 at mitochondria
- Author
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Giovanna Maria Pierantoni, Mara Tornincasa, Franca Esposito, Alfredo Fusco, G Chiappetta, and Antonio Federico
- Subjects
p53 ,Cancer Research ,Immunology ,Intrinsic apoptosis ,HMGA proteins ,HMGA ,apoptosis ,Cell Biology ,Plasma protein binding ,Mitochondrion ,Biology ,Cell biology ,mitochondria ,Cellular and Molecular Neuroscience ,High-mobility group ,Apoptosis ,Neoplastic transformation ,Original Article ,Bcl-2 ,HMGA Proteins - Abstract
The high-mobility group A (HMGA) proteins are a family of non-histone chromatin factors, encoded by the HMGA1 and HMGA2 genes. Several studies demonstrate that HMGA proteins have a critical role in neoplastic transformation, and their overexpression is mainly associated with a highly malignant phenotype, also representing a poor prognostic index. Even though a cytoplasmic localization of these proteins has been previously reported in some highly malignant neoplasias, a clear role for this localization has not been defined. Here, we first confirm the localization of the HMGA1 proteins in the cytoplasm of cancer cells, and then we report a novel mechanism through which HMGA1 inhibits p53-mitochondrial apoptosis by counteracting the binding of p53 to the anti-apoptotic factor Bcl-2. Indeed, we demonstrate a physical and functional interaction between HMGA1 and Bcl-2 proteins. This interaction occurs at mitochondria interfering with the ability of p53 protein to bind Bcl-2, thus counteracting p53-mediated mitochondrial apoptosis. This effect is associated with the inhibition of cytochrome c release and activation of caspases. Consistent with this mechanism, a strong correlation between HMGA1 cytoplasmic localization and a more aggressive histotype of thyroid, breast and colon carcinomas has been observed. Therefore, cytoplasmic localization of HMGA1 proteins in malignant tissues is a novel mechanism of inactivation of p53 apoptotic function.
- Published
- 2012
22. Production of transgenic mice expressing the Ki-ras oncogene under the control of a thyroglobulin promoter
- Author
-
G, Santelli, V, de Franciscis, G, Portella, G, Chiappetta, A, D'Alessio, D, Califano, R, Rosati, A, Mineo, C, Monaco, and G, Manzo
- Subjects
Adenoma ,Chloramphenicol O-Acetyltransferase ,Perchlorates ,Base Sequence ,Molecular Sequence Data ,Thyroid Gland ,Mice, Transgenic ,Polymerase Chain Reaction ,Sodium Compounds ,Gene Expression Regulation, Enzymologic ,Gene Expression Regulation, Neoplastic ,Mice ,Genes, ras ,Animals ,RNA, Messenger ,Thyroid Neoplasms ,In Situ Hybridization ,Amitrole - Abstract
Transgenic mice have been generated bearing three fusion genes consisting of: (a) a 900-base pair rat thyroglobulin promoter followed by a gene coding for a chloramphenicol acetyl transferase activity; (b) the same promoter followed by the complementary DNA of the human activated Ki-ras oncogene; (c) a 2000-base pair rat thyroglobulin promoter followed by the complementary DNA of the human activated Ki-ras. We have shown that the 900-base pair rat thyroglobulin promoter is able to direct the expression of the reporter gene specifically in the thyroid gland of transgenic mice. The mice bearing the two Ki-ras constructs, which express the transgene in thyroid glands, show thyroid abnormalities, although at very low incidence. These lesions appear after a long latency and with a benign aspect, thus suggest that, in agreement with literature data on naturally occurring human thyroid tumors, the action of an activated ras gene is not sufficient to attain a complete malignant conversion of thyroid glands in vivo. However, ras expression in thyroid follicular cells represents a favorable ground for tumor development, as shown by the fact that goitrogen stimulation experiments increase the occurrence of tumors.
- Published
- 1993
23. Thyroid specific expression of the Ki-ras oncogene in transgenic mice
- Author
-
G, Santelli, V, de Franciscis, G, Chiappetta, A, D'Alessio, D, Califano, A, Mineo, C, Monaco, and G, Vecchio
- Subjects
Mice ,Genes, ras ,Organ Specificity ,Thyroid Gland ,Animals ,Gene Expression ,Mice, Transgenic - Published
- 1993
24. Mammary carcinoma. A multiparametric (MDR-P-glycoprotein expression, regional node status, tumor cells kinetics and receptor status) and immunohistochemical study
- Author
-
G, Botti, A, De Matteis, G, Chiappetta, G, D'Aiuto, G, Esposito, and A, Picone
- Subjects
Kinetics ,Membrane Glycoproteins ,Receptors, Estrogen ,Lymphatic Metastasis ,Drug Resistance ,Humans ,Breast Neoplasms ,ATP Binding Cassette Transporter, Subfamily B, Member 1 ,Prognosis ,Immunohistochemistry ,Cell Division - Published
- 1990
25. Assessment of multidrug resistance (MDR) by immunohistochemistry in breast carcinoma. Correlation with tumor size and regional node status
- Author
-
G, Chiappetta, A M, Bevilacqua, A, Picone, G, Riccio, and G, Botti
- Subjects
Membrane Glycoproteins ,Lymphatic Metastasis ,Axilla ,Drug Resistance ,Humans ,Breast Neoplasms ,ATP Binding Cassette Transporter, Subfamily B, Member 1 ,Immunohistochemistry - Published
- 1990
26. [Clinico-hematological evaluation of 57 patients undergoing antiaggregant treatment with indobufen]
- Author
-
A, Andriani, G, Mosiello, M G, Chiappetta, G, Gregori, and P, Rinaldi
- Subjects
Male ,Time Factors ,Cardiovascular Diseases ,Humans ,Female ,Isoindoles ,Middle Aged ,Phenylbutyrates ,Platelet Aggregation Inhibitors - Abstract
A long-term administration of Indobufen, 400 mg daily, was evaluated in 57 patients with cardiovascular diseases. Aggregation waves induced by ADP, collagen and epinephrine showed a significant and persistent inhibition of platelet function. Minor side effects were observed and in 6 patients the drug was withdrawn: 4 patients began to experience gastric troubles, 1 patient had positive occult test for blood, 1 patient developed an allergic rush. Clinical evaluation and platelet aggregation study before starting indobufen and during the follow up period seem to be useful in the evaluation of effectiveness, safety, compliance and suitable daily dose.
- Published
- 1990
27. 95 DEVELOPMENT OF A QUANTITATIVE VANCOMYCIN IMMUNOASSAY FOR THE ABBOTT AxSYM ANALYZER
- Author
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M. Adamczyk, E. M. Brate, E. G. Chiappetta, S. Ginsburg, E. Hoffman, C. Klein, M. M. Perkowitz, S. D. Rege, P. P. Chou, and A. G. Costantino
- Subjects
Pharmacology ,Pharmacology (medical) - Published
- 1997
28. P927. Analysis of BRCA-1 mutations in families from Southern Italy
- Author
-
G. Esposito, E. Squame, Massimiliano D’Aiuto, G. Viglietto, Immacolata Capasso, G. Chiappetta, G.D.' Aiuto, Paola Bruni, M.T. Vento, M. Pizzorusso, and S. Buonagura
- Subjects
Genetics ,business.industry ,Medicine ,Surgery ,General Medicine ,business - Published
- 1997
29. HMGA1 protein over-expression is a frequent feature of epithelial ovarian carcinomas.
- Author
-
G. Viglietto, V. Masciullo, G. Scambia, G. Baldassarre, A. Fusco, F. Pentimalli, M.T. Berlingieri, A. Boccia, G. Chiappetta, J. Palazzo, G. Manfioletti, and V. Giancotti
- Subjects
OVARIAN cancer ,IMMUNOHISTOCHEMISTRY - Abstract
High mobility group A 1 (HMGA1) proteins are chromatinic factors, which are absent or expressed at very low levels in normal adult tissues, while they are over-expressed in several human malignant tumors. In this study, HMGA1 protein expression was investigated by immunohistochemistry in a series of 44 epithelial ovarian specimens, which included four normal ovarian tissues, 29 primary invasive carcinomas, one metastatic ovarian tumor and 10 low malignant potential (LMP) tumors. HMGA1 staining was not detected in normal ovarian surface epithelium, which is the area from which ovarian adenocarcinoma frequently arises. HMGA1 proteins were expressed at low levels in some LMP tumors, whereas they were present in abundance in most of the primary ovarian adenocarcinomas. RT-PCR and western blot analysis correlated with immunohistochemical data. We demonstrated that the suppression of HMGA1 protein synthesis by an adenovirus carrying the HMGA1 gene in antisense orientation (Ad-Yas-GFP) inhibited the growth of two human ovarian carcinoma cell lines (OVCAR-5 and OVCAR-8). These results confirm HMGA1 over-expression as a general feature of human malignant neoplasias, including ovarian cancer and suggest that suppression of HMGA1 protein synthesis by an antisense adenoviral vector may represent a new and promising gene therapy for the treatment of ovarian cancer. [ABSTRACT FROM AUTHOR]
- Published
- 2003
30. Immunologic Responses of Guinea Pigs to Dextran
- Author
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J R, Battisto, G, Chiappetta, and R, Hixon
- Subjects
Freund's Adjuvant ,Guinea Pigs ,Immunology ,Dextrans ,Precipitin Tests ,Antibodies ,Molecular Weight ,Immunity, Active ,Animals ,Immunology and Allergy ,Hypersensitivity, Delayed ,Lymph Nodes ,Antigens ,Spleen ,Skin Tests - Abstract
Summary A highly purified dextran of large molecular weight has been found to be antigenic in guinea pigs. Delayed dermal reactions and PCA antibody were produced upon immunization with this polysaccharide in Freund's complete adjuvant. The incidence of actively acquired immunity to this antigen is highest among guinea pigs of the Abyssinian strain. The majority of Pirbright, Hartley and family XIII guinea pigs do not become immunized to dextran. The ability of guinea pigs to actively develop immune responses to dextran has been tentatively interpreted as genetically determined. Adoptive transfer of the delayed dermal reactivity for dextran was accomplished with lymphoid cells but not serum. Transfers were successful from sensitive Abyssinian animals to normal Abyssinian animals (responders) but not to Pirbright animals (nonresponders). The inability of Pirbright animals to demonstrate delayed dermal responses to dextran following adoptive transfer of competent lymphoid cells has been viewed as a separate, second defect. The latter has been termed an inability of “translation.”
- Published
- 1968
31. [Simultaneous determination using gas chromatography of mestranol and norethisterone in estrogen-progestins combination for oral use]
- Author
-
G, Moretti, G, Cavina, G, Chiappetta, I, Fattori, M, Petrella, and V, Pompi
- Subjects
Drug Combinations ,Chromatography, Gas ,Mestranol ,Norethindrone ,Contraceptives, Oral ,Contraceptives, Oral, Hormonal - Abstract
The article describes a new method to determine the quantity of mestranol and of norethisterone in a combined oral contraceptive. Gas chromatography was used simultaneously to detect both agents, after they were extracted with ethyl acetate from the total compound of the tablet.
- Published
- 1977
32. [Platelet aggregation and antiaggregant drugs]
- Author
-
A, Andriani, M G, Chiappetta, and M, Fabiani
- Subjects
Thromboxane A2 ,Ticlopidine ,Platelet Aggregation ,Theophylline ,Verapamil ,Papaverine ,Prostaglandins ,Humans ,Dipyridamole ,Thiophenes - Published
- 1982
33. [The antiaggregation effect of ticlopidine on blood platelets preliminary results and prospectives for studies in various diseases]
- Author
-
A, Andriani, M G, Chiappetta, M, Fabiani, G, Mosiello, and A, Beni
- Subjects
Clinical Trials as Topic ,Ticlopidine ,Platelet Aggregation ,Anticoagulants ,Drug Evaluation ,Humans ,Thiophenes ,Middle Aged ,Aged - Published
- 1983
34. [Antiaggregating effect of indobufen. Preliminary results of a study of 20 patients]
- Author
-
A, Andriani, M G, Chiappetta, E, Mannarino, G, Mosiello, G, Zampa, and A, Beni
- Subjects
Adenosine Diphosphate ,Male ,Epinephrine ,Platelet Aggregation ,Humans ,Female ,Thrombosis ,Collagen ,Isoindoles ,Phenylbutyrates - Published
- 1986
35. Residenza, domicilio e dimora
- Author
-
Carolina Perlingieri, A. Alpini, V. Barba, G. Biscontini, E. Briganti, E. Capobianco, G. Carapezza Figlia G. Chiappetta, N. Cipriani, O. Clarizia, L. Corsaro, M.V. Cozzi, C. Crea F. Criscuolo, P. D’Addino, M. D’Ambrosio, P. D’Amico, A. Di Amato, S. Di Amato R. Di Raimo, V. Donato, C. Donisi, R. Favale, A. Federico, P. Femia, L. Ferroni A. Flamini, M. Galli, M. Imbrenda, F. Lazzarelli, G. Lisella, O. Lombardi L. Lonardo, F. Maisto, A. Malomo, D. Mantucci, G. Marinaro, E. Migliaccio E. Minervini, R. Mongillo, F. Nappi, A.C. Nazzaro, R. Pane, M. Pennasilico C. Perlingieri, G. Perlingieri, S. Polidori, I. Prisco, F. Prosperi, G. Recinto, V. Rizzo G. Romano, L. Rossi Carleo, F. Ruscello, T.V. Russo, F. Sbordone, P. Stanzione A. Tartaglia Polcini, G. Tatarano, B. Troisi, L. Tullio, M.A. Urciuoli D. Valentino, F. Volpe, G. Zarra, P. Perlingieri, and Perlingieri, Carolina
- Published
- 2021
36. Nascita ed esistenza
- Author
-
Carolina Perlingieri, A. Alpini, V. Barba, G. Biscontini, E. Briganti, E. Capobianco, G. Carapezza Figlia G. Chiappetta, N. Cipriani, O. Clarizia, L. Corsaro, M.V. Cozzi, C. Crea F. Criscuolo, P. D’Addino, M. D’Ambrosio, P. D’Amico, A. Di Amato, S. Di Amato R. Di Raimo, V. Donato, C. Donisi, R. Favale, A. Federico, P. Femia, L. Ferroni A. Flamini, M. Galli, M. Imbrenda, F. Lazzarelli, G. Lisella, O. Lombardi L. Lonardo, F. Maisto, A. Malomo, D. Mantucci, G. Marinaro, E. Migliaccio E. Minervini, R. Mongillo, F. Nappi, A.C. Nazzaro, R. Pane, M. Pennasilico C. Perlingieri, G. Perlingieri, S. Polidori, I. Prisco, F. Prosperi, G. Recinto, V. Rizzo G. Romano, L. Rossi Carleo, F. Ruscello, T.V. Russo, F. Sbordone, P. Stanzione A. Tartaglia Polcini, G. Tatarano, B. Troisi, L. Tullio, M.A. Urciuoli D. Valentino, F. Volpe, G. Zarra, P. Perlingieri, and Perlingieri, Carolina
- Published
- 2021
37. Nozione di matrimonio e sistema matrimoniale italiano
- Author
-
Carolina Perlingieri, A. Alpini, V. Barba, G. Biscontini, E. Briganti, E. Capobianco, G. Carapezza Figlia G. Chiappetta, N. Cipriani, O. Clarizia, L. Corsaro, M.V. Cozzi, C. Crea F. Criscuolo, P. D’Addino, M. D’Ambrosio, P. D’Amico, A. Di Amato, S. Di Amato R. Di Raimo, V. Donato, C. Donisi, R. Favale, A. Federico, P. Femia, L. Ferroni A. Flamini, M. Galli, M. Imbrenda, F. Lazzarelli, G. Lisella, O. Lombardi L. Lonardo, F. Maisto, A. Malomo, D. Mantucci, G. Marinaro, E. Migliaccio E. Minervini, R. Mongillo, F. Nappi, A.C. Nazzaro, R. Pane, M. Pennasilico C. Perlingieri, G. Perlingieri, S. Polidori, I. Prisco, F. Prosperi, G. Recinto, V. Rizzo G. Romano, L. Rossi Carleo, F. Ruscello, T.V. Russo, F. Sbordone, P. Stanzione A. Tartaglia Polcini, G. Tatarano, B. Troisi, L. Tullio, M.A. Urciuoli D. Valentino, F. Volpe, G. Zarra, P. Perlingieri, and Perlingieri, Carolina
- Published
- 2021
38. Modi acquisto della proprietà a titolo originario
- Author
-
Carolina Perlingieri, A. Alpini, V. Barba, G. Biscontini, E. Briganti, E. Capobianco, G. Carapezza Figlia G. Chiappetta, N. Cipriani, O. Clarizia, L. Corsaro, M.V. Cozzi, C. Crea F. Criscuolo, P. D’Addino, M. D’Ambrosio, P. D’Amico, A. Di Amato, S. Di Amato R. Di Raimo, V. Donato, C. Donisi, R. Favale, A. Federico, P. Femia, L. Ferroni A. Flamini, M. Galli, M. Imbrenda, F. Lazzarelli, G. Lisella, O. Lombardi L. Lonardo, F. Maisto, A. Malomo, D. Mantucci, G. Marinaro, E. Migliaccio E. Minervini, R. Mongillo, F. Nappi, A.C. Nazzaro, R. Pane, M. Pennasilico C. Perlingieri, G. Perlingieri, S. Polidori, I. Prisco, F. Prosperi, G. Recinto, V. Rizzo G. Romano, L. Rossi Carleo, F. Ruscello, T.V. Russo, F. Sbordone, P. Stanzione A. Tartaglia Polcini, G. Tatarano, B. Troisi, L. Tullio, M.A. Urciuoli D. Valentino, F. Volpe, G. Zarra, P. Perlingieri, and Perlingieri, Carolina
- Published
- 2021
39. La giurisdizione ecclesiastica e gli accertamenti del giudice statale in materia di matrimonio concordatario
- Author
-
Carolina Perlingieri, A. Alpini, V. Barba, G. Biscontini, E. Briganti, E. Capobianco, G. Carapezza Figlia G. Chiappetta, N. Cipriani, O. Clarizia, L. Corsaro, M.V. Cozzi, C. Crea F. Criscuolo, P. D’Addino, M. D’Ambrosio, P. D’Amico, A. Di Amato, S. Di Amato R. Di Raimo, V. Donato, C. Donisi, R. Favale, A. Federico, P. Femia, L. Ferroni A. Flamini, M. Galli, M. Imbrenda, F. Lazzarelli, G. Lisella, O. Lombardi L. Lonardo, F. Maisto, A. Malomo, D. Mantucci, G. Marinaro, E. Migliaccio E. Minervini, R. Mongillo, F. Nappi, A.C. Nazzaro, R. Pane, M. Pennasilico C. Perlingieri, G. Perlingieri, S. Polidori, I. Prisco, F. Prosperi, G. Recinto, V. Rizzo G. Romano, L. Rossi Carleo, F. Ruscello, T.V. Russo, F. Sbordone, P. Stanzione A. Tartaglia Polcini, G. Tatarano, B. Troisi, L. Tullio, M.A. Urciuoli D. Valentino, F. Volpe, G. Zarra, P. Perlingieri, and Perlingieri, Carolina
- Published
- 2021
40. Capacità giuridica. Soggettività. Personalità
- Author
-
Carolina Perlingieri, A. Alpini, V. Barba, G. Biscontini, E. Briganti, E. Capobianco, G. Carapezza Figlia G. Chiappetta, N. Cipriani, O. Clarizia, L. Corsaro, M.V. Cozzi, C. Crea F. Criscuolo, P. D’Addino, M. D’Ambrosio, P. D’Amico, A. Di Amato, S. Di Amato R. Di Raimo, V. Donato, C. Donisi, R. Favale, A. Federico, P. Femia, L. Ferroni A. Flamini, M. Galli, M. Imbrenda, F. Lazzarelli, G. Lisella, O. Lombardi L. Lonardo, F. Maisto, A. Malomo, D. Mantucci, G. Marinaro, E. Migliaccio E. Minervini, R. Mongillo, F. Nappi, A.C. Nazzaro, R. Pane, M. Pennasilico C. Perlingieri, G. Perlingieri, S. Polidori, I. Prisco, F. Prosperi, G. Recinto, V. Rizzo G. Romano, L. Rossi Carleo, F. Ruscello, T.V. Russo, F. Sbordone, P. Stanzione A. Tartaglia Polcini, G. Tatarano, B. Troisi, L. Tullio, M.A. Urciuoli D. Valentino, F. Volpe, G. Zarra, P. Perlingieri, and Perlingieri, Carolina
- Published
- 2021
41. Riservatezza e banche dati
- Author
-
Carolina Perlingieri, A. Alpini, V. Barba, G. Biscontini, E. Briganti, E. Capobianco, G. Carapezza Figlia G. Chiappetta, N. Cipriani, O. Clarizia, L. Corsaro, M.V. Cozzi, C. Crea F. Criscuolo, P. D’Addino, M. D’Ambrosio, P. D’Amico, A. Di Amato, S. Di Amato R. Di Raimo, V. Donato, C. Donisi, R. Favale, A. Federico, P. Femia, L. Ferroni A. Flamini, M. Galli, M. Imbrenda, F. Lazzarelli, G. Lisella, O. Lombardi L. Lonardo, F. Maisto, A. Malomo, D. Mantucci, G. Marinaro, E. Migliaccio E. Minervini, R. Mongillo, F. Nappi, A.C. Nazzaro, R. Pane, M. Pennasilico C. Perlingieri, G. Perlingieri, S. Polidori, I. Prisco, F. Prosperi, G. Recinto, V. Rizzo G. Romano, L. Rossi Carleo, F. Ruscello, T.V. Russo, F. Sbordone, P. Stanzione A. Tartaglia Polcini, G. Tatarano, B. Troisi, L. Tullio, M.A. Urciuoli D. Valentino, F. Volpe, G. Zarra, P. Perlingieri, and Perlingieri, Carolina
- Published
- 2021
42. Diritto all'oblio
- Author
-
Carolina Perlingieri, A. Alpini, V. Barba, G. Biscontini, E. Briganti, E. Capobianco, G. Carapezza Figlia G. Chiappetta, N. Cipriani, O. Clarizia, L. Corsaro, M.V. Cozzi, C. Crea F. Criscuolo, P. D’Addino, M. D’Ambrosio, P. D’Amico, A. Di Amato, S. Di Amato R. Di Raimo, V. Donato, C. Donisi, R. Favale, A. Federico, P. Femia, L. Ferroni A. Flamini, M. Galli, M. Imbrenda, F. Lazzarelli, G. Lisella, O. Lombardi L. Lonardo, F. Maisto, A. Malomo, D. Mantucci, G. Marinaro, E. Migliaccio E. Minervini, R. Mongillo, F. Nappi, A.C. Nazzaro, R. Pane, M. Pennasilico C. Perlingieri, G. Perlingieri, S. Polidori, I. Prisco, F. Prosperi, G. Recinto, V. Rizzo G. Romano, L. Rossi Carleo, F. Ruscello, T.V. Russo, F. Sbordone, P. Stanzione A. Tartaglia Polcini, G. Tatarano, B. Troisi, L. Tullio, M.A. Urciuoli D. Valentino, F. Volpe, G. Zarra, P. Perlingieri, and Perlingieri, Carolina
- Published
- 2021
43. Diritti della personalità ed enti collettivi
- Author
-
PERLINGIERI, CAROLINA, A. Alpini, V. Barba, G. Biscontini, E. Briganti, E. Capobianco, G. Carapezza Figlia G. Chiappetta, N. Cipriani, O. Clarizia, L. Corsaro, M.V. Cozzi, C. Crea F. Criscuolo, P. D’Addino, M. D’Ambrosio, P. D’Amico, A. Di Amato, S. Di Amato R. Di Raimo, V. Donato, C. Donisi, R. Favale, A. Federico, P. Femia, L. Ferroni A. Flamini, M. Galli, M. Imbrenda, F. Lazzarelli, G. Lisella, O. Lombardi L. Lonardo, F. Maisto, A. Malomo, D. Mantucci, G. Marinaro, E. Migliaccio E. Minervini, R. Mongillo, F. Nappi, A.C. Nazzaro, R. Pane, M. Pennasilico C. Perlingieri, G. Perlingieri, S. Polidori, I. Prisco, F. Prosperi, G. Recinto, V. Rizzo G. Romano, L. Rossi Carleo, F. Ruscello, T.V. Russo, F. Sbordone, P. Stanzione A. Tartaglia Polcini, G. Tatarano, B. Troisi, L. Tullio, M.A. Urciuoli D. Valentino, F. Volpe, G. Zarra, P. Perlingieri, Perlingieri, Carolina, and Pietro Perlingieri
- Subjects
diritti ,persona ,enti ,enti non riconosciuti - Abstract
diritti della personalità ed enti non riconosciuti
- Published
- 2021
44. Incapacità d'intendere o di volere
- Author
-
Carolina Perlingieri, A. Alpini, V. Barba, G. Biscontini, E. Briganti, E. Capobianco, G. Carapezza Figlia G. Chiappetta, N. Cipriani, O. Clarizia, L. Corsaro, M.V. Cozzi, C. Crea F. Criscuolo, P. D’Addino, M. D’Ambrosio, P. D’Amico, A. Di Amato, S. Di Amato R. Di Raimo, V. Donato, C. Donisi, R. Favale, A. Federico, P. Femia, L. Ferroni A. Flamini, M. Galli, M. Imbrenda, F. Lazzarelli, G. Lisella, O. Lombardi L. Lonardo, F. Maisto, A. Malomo, D. Mantucci, G. Marinaro, E. Migliaccio E. Minervini, R. Mongillo, F. Nappi, A.C. Nazzaro, R. Pane, M. Pennasilico C. Perlingieri, G. Perlingieri, S. Polidori, I. Prisco, F. Prosperi, G. Recinto, V. Rizzo G. Romano, L. Rossi Carleo, F. Ruscello, T.V. Russo, F. Sbordone, P. Stanzione A. Tartaglia Polcini, G. Tatarano, B. Troisi, L. Tullio, M.A. Urciuoli D. Valentino, F. Volpe, G. Zarra, P. Perlingieri, and Perlingieri, Carolina
- Published
- 2021
45. Minore età: dalla potestà dei genitori alla responsabilità genitoriale
- Author
-
Carolina Perlingieri, A. Alpini, V. Barba, G. Biscontini, E. Briganti, E. Capobianco, G. Carapezza Figlia G. Chiappetta, N. Cipriani, O. Clarizia, L. Corsaro, M.V. Cozzi, C. Crea F. Criscuolo, P. D’Addino, M. D’Ambrosio, P. D’Amico, A. Di Amato, S. Di Amato R. Di Raimo, V. Donato, C. Donisi, R. Favale, A. Federico, P. Femia, L. Ferroni A. Flamini, M. Galli, M. Imbrenda, F. Lazzarelli, G. Lisella, O. Lombardi L. Lonardo, F. Maisto, A. Malomo, D. Mantucci, G. Marinaro, E. Migliaccio E. Minervini, R. Mongillo, F. Nappi, A.C. Nazzaro, R. Pane, M. Pennasilico C. Perlingieri, G. Perlingieri, S. Polidori, I. Prisco, F. Prosperi, G. Recinto, V. Rizzo G. Romano, L. Rossi Carleo, F. Ruscello, T.V. Russo, F. Sbordone, P. Stanzione A. Tartaglia Polcini, G. Tatarano, B. Troisi, L. Tullio, M.A. Urciuoli D. Valentino, F. Volpe, G. Zarra, P. Perlingieri, and Perlingieri, Carolina
- Published
- 2021
46. Diritto alla riservatezza
- Author
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Carolina Perlingieri, A. Alpini, V. Barba, G. Biscontini, E. Briganti, E. Capobianco, G. Carapezza Figlia G. Chiappetta, N. Cipriani, O. Clarizia, L. Corsaro, M.V. Cozzi, C. Crea F. Criscuolo, P. D’Addino, M. D’Ambrosio, P. D’Amico, A. Di Amato, S. Di Amato R. Di Raimo, V. Donato, C. Donisi, R. Favale, A. Federico, P. Femia, L. Ferroni A. Flamini, M. Galli, M. Imbrenda, F. Lazzarelli, G. Lisella, O. Lombardi L. Lonardo, F. Maisto, A. Malomo, D. Mantucci, G. Marinaro, E. Migliaccio E. Minervini, R. Mongillo, F. Nappi, A.C. Nazzaro, R. Pane, M. Pennasilico C. Perlingieri, G. Perlingieri, S. Polidori, I. Prisco, F. Prosperi, G. Recinto, V. Rizzo G. Romano, L. Rossi Carleo, F. Ruscello, T.V. Russo, F. Sbordone, P. Stanzione A. Tartaglia Polcini, G. Tatarano, B. Troisi, L. Tullio, M.A. Urciuoli D. Valentino, F. Volpe, G. Zarra, P. Perlingieri, and Perlingieri, Carolina
- Published
- 2021
47. La proporzionalità e la ragionevolezza nella proposta di regolamento di un diritto comune europeo della vendita
- Author
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GIOVANNA D'ALFONSO, A. Alpini, M. Angelone, V. Barba, C. Buccico, G. Carapezza Figlia, G. Chiappetta, C. Crea, G. D'Alfonso, C. De Fiores, A. De Franceschi, V. De Oto, F. Di Porto, D. Di Sabato, J. Estruch, A. Fachechi, M. Francesca, F. Gambardella, E. Giorgini, L. Lamberti, F. Lazzarelli, A. Lepore, L. Letizia, F. Maisto, A. Malomo, A. Mignozzi, E. Minervini, G. Palmieri, A. Pastena, A. Patroni Griffi, M. Pennasilico, G. Perlingieri, G. Petrillo, S. Polidori, F. Quarta, G. Rotondo, G. D. Ruggiero, G. Salvi, R. Santagata de Castro, S. Sajeva, G. Tepedino, S. Triggiani, M.C. Vitucci, G. Perlingieri A. Fachechi, and D'Alfonso, Giovanna
- Subjects
Proporzionalità, Ragionevolezza, Proposta di regolamento di un diritto comune europeo, giustizia contrattuale - Abstract
Il contributo esamina la portata che il principio di proporzionalità ed il criterio ermeneutico-argomentativo di ragionevolezza assumono nella Proposta di Regolamento del diritto comune europeo della vendita. L’analisi assume preminente interesse, poiché il provvedimento, pur non essendo stato approvato, rappresenta un modello innovativo di regolamentazione uniforme, ispirato al principio di “giustizia contrattuale”.
- Published
- 2017
48. An increased high-mobility group A2 expression level is associated with malignant phenotype in pancreatic exocrine tissue
- Author
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N Matsumoto, Yutaka Atomi, Toshiyuki Mori, N. Abe, Masanori Sugiyama, Yutaka Suzuki, Tadahiko Masaki, Takashi Watanabe, Alfredo Fusco, Gennaro Chiappetta, N., Abe, T., Watanabe, Y., Suzuki, N., Matsumoto, T., Masaki, T., Mori, M., Sugiyama, G., Chiappetta, Fusco, Alfredo, Y., Atomi, Abe, N, Watanabe, T, Suzuki, Y, Matsumoto, N, Masaki, T, Mori, T, Sugiyama, M, Chiappetta, G, and Atomi, Y.
- Subjects
Cancer Research ,Pathology ,medicine.medical_specialty ,HMGA2 ,Pancreatic disease ,pancreatic cancer ,Biology ,Pancreatic cancer ,medicine ,Carcinoma ,Humans ,RNA, Messenger ,Pancreas ,immunostaining ,Reverse Transcriptase Polymerase Chain Reaction ,HMGA2 Protein ,Molecular and Cellular Pathology ,medicine.disease ,Immunohistochemistry ,RT–PCR ,Pancreatic Neoplasms ,Reverse transcription polymerase chain reaction ,Real-time polymerase chain reaction ,Pancreatitis ,Oncology ,Cancer research ,Immunostaining ,Carcinoma, Pancreatic Ductal - Abstract
The altered form of the high-mobility group A2 (HMGA2) gene is somehow related to the generation of human benign and malignant tumours of mesenchymal origin. However, only a few data on the expression of HMGA2 in malignant tumour originating from epithelial tissue are available. In this study, we examined the HMGA2 expression level in pancreatic carcinoma, and investigated whether alterations in the HMGA2 expression level are associated with a malignant phenotype in pancreatic tissue. High-mobility group A2 mRNA and protein expression was determined in eight surgically resected specimens of non-neoplastic tissue (six specimens of normal pancreatic tissue and two of chronic pancreatitis tissue) and 27 pancreatic carcinomas by highly sensitive reverse transcriptase-polymerase chain reaction (RT-PCR) techniques and immunohistochemical staining, respectively. Reverse transcriptase-polymerase chain reaction analysis revealed the expression of the HMGA2 gene in non-neoplastic pancreatic tissue, although its expression level was significantly lower than that in carcinoma. Immunohistochemical analysis indicated that the presence of the HMGA2 gene in non-neoplastic pancreatic tissue observed in RT-PCR reflects its abundant expression in islet cells, together with its focal expression in duct epithelial cells. Intense and multifocal or diffuse HMGA2 immunoreactivity was noted in all the pancreatic carcinoma examined. A strong correlation between HMGA2 overexpression and the diagnosis of carcinoma was statistically verified. Based on these findings, we propose that an increased expression level of the HMGA2 protein is closely associated with the malignant phenotype in the pancreatic exocrine system, and accordingly, HMGA2 could serve as a potential diagnostic molecular marker for distinguishing pancreatic malignant cells from non-neoplastic pancreatic exocrine cells.
- Published
- 2003
49. HMGA1 protein over-expression is a frequent feature of epithelial ovarian carcinomas
- Author
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Giuseppe Viglietto, Francesca Pentimalli, Gustavo Baldassarre, Giovanni Scambia, Juan P. Palazzo, Guidalberto Manfioletti, Vincenzo Giancotti, Angelo Boccia, Alfredo Fusco, Maria Teresa Berlingieri, Gennaro Chiappetta, Valeria Masciullo, V., Masciullo, G., Baldassarre, F., Pentimalli, M. T., Berlingieri, A., Boccia, G., Chiappetta, J., Palazzo, G., Manfioletti, V., Giancotti, G., Viglietto, G., Scambia, and Fusco, Alfredo
- Subjects
Cancer Research ,endocrine system diseases ,Adenocarcinoma ,Biology ,DNA, Antisense ,Adenoviridae ,Immunoenzyme Techniques ,Ovarian tumor ,Western blot ,Ovarian carcinoma ,Tumor Cells, Cultured ,medicine ,Carcinoma ,Humans ,HMGA1a Protein ,Adaptor Proteins, Signal Transducing ,DNA Primers ,GRB2 Adaptor Protein ,Ovarian Neoplasms ,medicine.diagnostic_test ,Reverse Transcriptase Polymerase Chain Reaction ,Proteins ,General Medicine ,medicine.disease ,HMGA1 ,female genital diseases and pregnancy complications ,Epithelium ,Gene Expression Regulation, Neoplastic ,medicine.anatomical_structure ,biology.protein ,Cancer research ,Immunohistochemistry ,Female ,Ovarian cancer - Abstract
High mobility group A 1 (HMGA1) proteins are chromatinic factors, which are absent or expressed at very low levels in normal adult tissues, while they are over-expressed in several human malignant tumors. In this study, HMGA1 protein expression was investigated by immunohistochemistry in a series of 44 epithelial ovarian specimens, which included four normal ovarian tissues, 29 primary invasive carcinomas, one metastatic ovarian tumor and 10 low malignant potential (LMP) tumors. HMGA1 staining was not detected in normal ovarian surface epithelium, which is the area from which ovarian adenocarcinoma frequently arises. HMGA1 proteins were expressed at low levels in some LMP tumors, whereas they were present in abundance in most of the primary ovarian adenocarcinomas. RT-PCR and western blot analysis correlated with immunohistochemical data. We demonstrated that the suppression of HMGA1 protein synthesis by an adenovirus carrying the HMGA1 gene in antisense orientation (Ad-Yas-GFP) inhibited the growth of two human ovarian carcinoma cell lines (OVCAR-5 and OVCAR-8). These results confirm HMGA1 over-expression as a general feature of human malignant neoplasias, including ovarian cancer and suggest that suppression of HMGA1 protein synthesis by an antisense adenoviral vector may represent a new and promising gene therapy for the treatment of ovarian cancer.
- Published
- 2003
50. Diagnostic Significance of High Mobility Group I(Y) Protein Expression in Intraductal Papillary Mucinous Tumors of the Pancreas
- Author
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Masanori Sugiyama, Gennaro Chiappetta, Yasunori Fujioka, Toshiyuki Mori, Yumi Izumisato, Alfredo Fusco, Yutaka Atomi, Nobutsugu Abe, Tadahiko Masaki, Takashi Watanabe, N., Abe, T., Watanabe, Y., Izumisato, T., Masaki, T., Mori, M., Sugiyama, G., Chiappetta, Fusco, Alfredo, Y., Fujioka, and Y., Atomi
- Subjects
Mild Dysplasia ,Pathology ,medicine.medical_specialty ,Pancreatic disease ,Hepatology ,Endocrinology, Diabetes and Metabolism ,Biology ,medicine.disease ,Adenocarcinoma, Mucinous ,Immunohistochemistry ,Carcinoma, Papillary ,Malignant transformation ,Pancreatic Neoplasms ,Endocrinology ,medicine.anatomical_structure ,Internal Medicine ,medicine ,Carcinoma ,Humans ,Adenocarcinoma ,HMGA1a Protein ,Mucinous Tumor ,Pancreas ,Carcinoma, Pancreatic Ductal ,Moderate Dysplasia - Abstract
Introduction Overexpression of the high mobility group I(Y), [HMGI(Y)], gene/proteins has been demonstrated in many types of human malignancies, suggesting that HMGI(Y) may play a vital role in the oncogenic transformation of cells. Aims To analyze HMGI(Y) expression in intraductal papillary mucinous tumor (IPMT) of the pancreas to verify whether determination of the HMGI(Y) expression level could provide any diagnostic advantages in the pathological diagnosis of IPMT. Methodology Thirty-three different lesions from 25 patients with IPMT, including 20 with mild dysplasia, 7 with moderate dysplasia, and 6 with carcinoma, were analyzed immunohistochemically with use of an HMGI(Y)-specific antibody. Results Immunohistochemical analysis revealed that, although no significant immunoreactivity was found in cases of normal pancreatic duct or mild dysplasia, 28.6% (2/7) of moderate dysplasia showed multifocal immunoreactivity with moderate intensity. In contrast, in 50% (3/6) of the cases of carcinoma, intense multifocal or diffuse immunoreactivity occurred, almost equivalent to that observed in cases of duct cell carcinoma, whereas in the remaining 3 cases of carcinoma only a faint focal immunoreactivity occurred. Histologic examination revealed that these HMGI(Y)-positive carcinomas had an invasive growth pattern, whereas the HMGI(Y)-negative carcinomas were either carcinomas in situ or tumors with minimal invasion. Thus, an increased expression level of HMGI(Y) proteins was closely associated with the malignant phenotype in IPMT. Conclusion On the basis of these findings, we propose that HMGI(Y) proteins could play an important role(s) in a multistage process of carcinogenesis of IPMT and that the HMGI(Y) protein level could serve as a potential diagnostic marker, which may enable the identification of tumor cells with potential to be biologically malignant.
- Published
- 2002
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