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Increase in AP-1 activity is a general event in thyroid cell transformation in vitro and in vivo
- Source :
- Oncogene (Basingstoke) (1998)., info:cnr-pdr/source/autori:Battista S, de Nigris F, Fedele M, Chiappetta G, Scala S, Vallone D, Pierantoni GM, Mega T, Santoro M, Viglietto G, Verde P, Fusco A./titolo:Increase in AP-1 activity is a general event in thyroid cell transformation in vitro and in vivo./doi:/rivista:Oncogene (Basingstoke)/anno:1998/pagina_da:/pagina_a:/intervallo_pagine:/volume, Scopus-Elsevier
- Publication Year :
- 1998
- Publisher :
- Macmillan, Basingstoke , Regno Unito, 1998.
-
Abstract
- We have recently reported that neoplastic transformation of two rat thyroid epithelial cell lines by retroviruses carrying the v-mos and v-ras Ki oncogenes is associated with a drastic increase of AP-1 activity. The most important effects were represented by the dramatic junB and fra-1 gene induction, which was abolished by the block of the transformation-induced HMGI-C protein synthesis. Here, we have further characterized the transformation-dependent AP-1 activity, by analysing the expression of different jun- and fos-related components, in rat thyroid cell lines transformed by several oncogenes, in human thyroid carcinoma cell lines, and in naturally occurring human thyroid tumours. A significant increase of Fra-1 and JunB protein levels was detected in all oncogene transformed rat thyroid cell lines. Fra-1 gene induction was demonstrated to occur also in human thyroid carcinoma cell lines and tissues. Conversely, c-Jun and JunD proteins, rather than JunB, accumulated in human thyroid carcinoma cell lines. An induction of AP-1 target genes was also detected both in rat and human thyroid transformed cell lines. Therefore, in vivo and in vitro thyroid cell transformation is associated with important compositional changes in the AP-1 complex and an increased transcriptional activity.
- Subjects :
- endocrine system
Cancer Research
medicine.medical_specialty
endocrine system diseases
JUNB
Proto-Oncogene Proteins c-jun
Thyroid Gland
Thyrotropin
Biology
Polymerase Chain Reaction
Thyroglobulin
Thyroid carcinoma
Genes, jun
In vivo
Internal medicine
Genetics
medicine
Animals
Humans
Neoplastic transformation
Thyroid Neoplasms
Molecular Biology
Oncogene
Thyroid
HMGA2 Protein
High Mobility Group Proteins
Epithelial Cells
Oncogenes
In vitro
Neoplasm Proteins
Rats
Transcription Factor AP-1
Endocrinology
medicine.anatomical_structure
Cell Transformation, Neoplastic
Cell culture
Cancer research
Proto-Oncogene Proteins c-fos
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Oncogene (Basingstoke) (1998)., info:cnr-pdr/source/autori:Battista S, de Nigris F, Fedele M, Chiappetta G, Scala S, Vallone D, Pierantoni GM, Mega T, Santoro M, Viglietto G, Verde P, Fusco A./titolo:Increase in AP-1 activity is a general event in thyroid cell transformation in vitro and in vivo./doi:/rivista:Oncogene (Basingstoke)/anno:1998/pagina_da:/pagina_a:/intervallo_pagine:/volume, Scopus-Elsevier
- Accession number :
- edsair.doi.dedup.....c5d94c88cfb8bf05e766f5c25145020e