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1. Contribution of Psychological Autopsy to the Understanding of Suicidal Behaviors in French Overseas Territories (AUTOPSOM)

Author: Department for Research, Studies, Evaluation and Statistics, French Health and Social Affairs Ministry, France, French National Institute for Health and Medical Research, MOODS Unit, Clinical research and Innovation Office, Martinique, SOS KRIZ, Martinique, Amiens University Hospital, France, Suicide Prevention Resource Center, French Guiana, Cayenne Hospital Center, French Guiana, University Hospital, La Réunion, Tahiti Hospital Center, French Polynesia, Regional Health Agency, Martinique, Regional Health Agency, French Guiana, Regional Health Agency, La Somme, and Regional Health Agency, La Réunion
Published: 2024

2. Cardiovascular Risk Prevention Among Night Workers (Heart-Of-Night)

Author: assistance of the French National Health Insurance Fund for Employees (CNAMTS), French Directorate General of Health (DGS), ARC Foundation for Cancer Research, National Cancer Institute, France, French National Institute for Prevention and Education in Health (INPES), Institut National de la Santé Et de la Recherche Médicale, France, French Inter-Departemental Agency for the Fight against Drugs and Addictive Behaviors (Mildeca), and French Social Security Scheme for Liberal Professionals (RSI)
Published: 2024

3. Word Importance Discrimination using Context Information

Author: FRENCH NATIONAL INSTITUTE FOR RESEARCH IN COMPUTER SCIENCE AND CONTROL SOPHIA ANTIPOLIS- MEDITERRANEAN RESEARCH CENTER (FRANCE), Nemirovsky, Danil, Dobrynin, Vladimir, FRENCH NATIONAL INSTITUTE FOR RESEARCH IN COMPUTER SCIENCE AND CONTROL SOPHIA ANTIPOLIS- MEDITERRANEAN RESEARCH CENTER (FRANCE), Nemirovsky, Danil, and Dobrynin, Vladimir
Abstract: Word importance discrimination is a task deserving attention when one treats a topic from TREC where a topic is quite long. The goal of the process is to estimate importance of words which carry any (additional) information about user information needs. In our experiments we estimated word importance using context information of a word., Presented at the Text REtrieval Conference (TREC 2008) (17th) held in Gaithersburg, Maryland on 18-21 November 2008. Published in the Text REtrieval Conference Proceedings, p1-7, November 2008. Sponsored in part by DARPA and ARDA. The original document contains color images.
Published: 2008

4. Regional analysis of road mortality in Europe

Author: French National Institute for Road Safety Research - Group for the Analysis of Road Risk and its Governance, UCL - EUEN/CORE - Center for operations research and econometrics, Eksler, Vojtech, Lassarre, Sylvain, Thomas, Isabelle, French National Institute for Road Safety Research - Group for the Analysis of Road Risk and its Governance, UCL - EUEN/CORE - Center for operations research and econometrics, Eksler, Vojtech, Lassarre, Sylvain, and Thomas, Isabelle
Abstract: Background: Road accidents are the tenth leading cause of premature death worldwide and, based on current trends, are likely to become the third leading cause of disability-adjusted life years lost by 2020. Road mortality varies in time and space both between countries and also between regions within the same country. Identifying and understanding the background of regional differences may lead to better understanding of the sources of road accidents, and enable the application of more effective road safety policies. Methods: A Bayesian ecological regression model based on a unified generalized linear mixed model framework is introduced. Population density and country (affiliation) were used as covariates and were fitted into the model at the four levels of spatial aggregation known as ‘nomenclature of statistical territorial units (NUTS) regional classification’. Results: Population density has a significant influence on road mortality. For all countries together, the elasticity estimate is -0.32, meaning that a 10% increase in population density is linked to a 3.2% decrease in road fatalities. A multi-level model defined at the NUTS-3 level, taking into account the NUTS-2 aggregation, enables infraregional variances in road mortality to be taken into account and produces the most reliable estimates of the model parameters. Variation in the Bayes relative risk (the mortality ratio ‘standardized’ by population density and country effect) is highest at the NUTS-3 level, but is lower at country level and NUTS-2 level, which suggests that other important underlying factors are responsible for the variations in road mortality between regions. Mapping the Bayes relative risk enables the identification of regions that should be targeted by national and regional policies. Last but not least, a new ranking of European countries according to their road mortality risk, adjusted for population density, is presented.
Published: 2008

5. Prenatal and childhood exposure to ambient air pollution and cognitive function in school-age children: Examining sensitive windows and sex-specific associations

Author: Guilbert, Ariane, Bernard, Jonathan Y., Peyre, Hugo, Costet, Nathalie, Hough, Ian, Seyve, Emie, Monfort, Christine, Philippat, Claire, Slama, Rémy, Kloog, Itai, Chevrier, Cécile, Heude, Barbara, Ramus, Franck, Lepeule, Johanna, Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre de recherche en épidémiologie et santé des populations (CESP), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, CHU Montpellier, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), École des Hautes Études en Santé Publique [EHESP] (EHESP), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Ben-Gurion University of the Negev (BGU), Institut des Géosciences de l’Environnement (IGE), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA), Laboratoire de sciences cognitives et psycholinguistique (LSCP), Département d'Etudes Cognitives - ENS Paris (DEC), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École des hautes études en sciences sociales (EHESS)-Centre National de la Recherche Scientifique (CNRS), This work as well as AG were funded by the Fondation de France (PAND-Alp grant 00089597).The EDEN cohort was supported by the Foundation for Medical Research, the National Agency for Research, the National Institute for Research in Public Health (IRESP: TGIR cohorte santé. 2008 program), the French Ministry of Health, the French Ministry of Research, the Inserm Bone and Joint Diseases National Research and Human Nutrition National Research Programs, Paris-Sud University, Nestlé, the French National Institute for Population Health Surveillance, the French National Institute for Health Education, the European Union FP7 programs (FP7/2007–2013, HELIX, ESCAPE, ENRIECO, Medall projects), the Diabetes National Research Program (through a collaboration with the French Association of Diabetic Patients), the French Agency for Environmental Health Safety, MutuelleGénérale de l’Education Nationale, the French National Agency for Food Security and the French-speaking Association for the Study of Diabetes and Metabolism.This study in the PELAGIE cohort was mainly supported by Inserm, the French National Research Agency (ANR-10-PRSP-0007) and the French Agency for Food, Environmental and Occupational Health and Safety (ANSES APR EST, 2009). The initial fundings of the PELAGIE cohort were Inserm, the French Ministries of Health, Labor, and Research, the French National Institute for Public Health Surveillance, the National Agency for Research, the French Agency for Environmental Health Safety (Afsset/ANSES), the European programs Hi-WATE and ENRIECO, and the Research Institute of Public Health.FR and HP received support under the program 'Investissements d’Avenir' launched by the French Government and implemented by ANR with the references ANR-17-EURE-0017 and ANR-10-IDEX-0001-02.The funders contributed to data collection but had no role in the design and conduct of the study, analysis, and interpretation of the data, preparation, review, or approval of the manuscript, and decision to submit the manuscript for publication. The opinions, results, and conclusions herein are those of the authors and do not reflect the official policy or position of the funding sources., ANR-10-IDEX-0001,PSL,Paris Sciences et Lettres(2010), ANR-17-EURE-0017,FrontCog,Frontières en cognition(2017), and ANR-10-PRSP-0007,PEPSY(2010)
Subjects: DLNM, PM, IQ, Pregnancy, [SDV]Life Sciences [q-bio], Neurodevelopment, NO(2)
Abstract: International audience; BACKGROUND: Combined effect of both prenatal and early postnatal exposure to ambient air pollution on child cognition has rarely been investigated and sensitive periods of sensitivity are unknown. This study explores the temporal relationship between pre- and postnatal exposure to PM(10), PM(2.5), NO(2) and child cognitive function. METHODS: Using validated spatiotemporally resolved exposure models, pre- and postnatal daily PM(2.5), PM(10) (satellite based, 1 km resolution) and NO(2) (chemistry-transport model, 4 km resolution) concentrations at the mother’s residence were estimated for 1271 mother-child pairs from the French EDEN and PELAGIE cohorts. Scores representative of children’s General, Verbal and Non-Verbal abilities at 5-6 years were constructed based on subscale scores from the WPPSI-III, WISC-IV or NEPSY-II batteries, using confirmatory factor analysis (CFA). Associations of both prenatal (first 35 gestational weeks) and postnatal (60 months after birth) exposure to air pollutants with child cognition were explored using Distributed Lag Non-linear Models adjusted for confounders. RESULTS: Median exposure from conception until the 60th month of life was 19.3 μg/m(3) for PM(10), 12.4 μg/m(3) for PM(2.5) and 16.9 μg/m(3) for NO(2). Increased maternal exposure to both PM(10) and PM(2.5) between the 5th and the 11th gestational weeks was related to higher General, Verbal and Non-verbal abilities among males. On the contrary, increased maternal exposure to PM(10) between the 22nd and 29th gestational weeks was associated with lower General and Non-verbal abilities among males. Similar trends were observed for PM(2.5). No significant sensitive exposure windows were detected for postnatal exposure, NO(2) or among females. DISCUSSION: These results suggest poorer cognitive development among males at 5-6 years following increased maternal exposure to PM(10) during mid-pregnancy. Apparent protective associations observed for early prenatal exposure to PM(10) and PM(2.5) are unlikely to be causal and might be due to live birth selection bias.
Published: 2023
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6. Infant feeding practices associated with adiposity peak and rebound in the EDEN mother–child cohort

Author: Aurore Camier, Aminata H. Cissé, Sandrine Lioret, Jonathan Y. Bernard, Marie Aline Charles, Barbara Heude, Blandine de Lauzon-Guillain, de Lauzon-Guillain, Blandine, Influence de l'alimentation infantile sur la croissance et le développement de l'enfant - - InfaDiet2019 - ANR-19-CE36-0008 - AAPG2019 - VALID, Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Singapore Institute for Clinical Sciences, Agency for science, technology and research [Singapore] (A*STAR), Etude longitudinale française depuis l'enfance (UMS : Ined-Inserm-EFS) (ELFE), Institut national d'études démographiques (INED)-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), The EDEN study is supported by the Fondation pour la Recherche Médicale (FRM), French Ministry of Research: Federative Research Institutes and Cohort Program, INSERM Human Nutrition National Research Program, and Diabetes National Research Program (by a collaboration with the French Association of Diabetic Patients [AFD]), French Ministry of Health, French Agency for Environment Security (AFSSET), French National Institute for Population Health Surveillance (InVS), Paris‐Sud University, French National Institute for Health Education (INPES), Nestlé, Mutuelle Générale de l'Education Nationale (MGEN), French‐speaking Association for the Study of Diabetes and Metabolism (ALFEDIAM), National Agency for Research (ANR non‐thematic programme), and National Institute for Research in Public Health (IRESP: TGIR 2008 cohort in health programme).The study was funded by an ANR grant (InfaDiet project, grant no.: ANR-19-CE36-0008).This research benefited from the assistance of the funding partners of the IReSP within the framework of the 2016 General call for projects - Prevention topic (HEUDE-AAP16-PREV-24)., and ANR-19-CE36-0008,InfaDiet,Influence de l'alimentation infantile sur la croissance et le développement de l'enfant(2019)
Subjects: Male, Nutrition and Dietetics, infant feeding, breastfeeding, Endocrinology, Diabetes and Metabolism, Infant, Newborn, Infant, Medicine (miscellaneous), Mother-Child Relations, Body Mass Index, Cohort Studies, BMI, Breast Feeding, adiposity rebound, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, Humans, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Female, early growth, Obesity, Adiposity
Abstract: International audience; Background/ObjectiveHigh magnitude of adiposity peak and early adiposity rebound are early risk markers of later obesity. Infant diet represents one of the main modifiable determinants of early growth. This study aimed to investigate the association between infant feeding practices and age and magnitude of adiposity peak and rebound.Subjects/MethodsAnalyses were based on data from the French EDEN mother–child cohort. Data on breastfeeding and complementary feeding were collected at birth and 4, 8 and 12 months. From clinical examinations and measurements collected in the child’s health booklet up to 12 years, individual growth curves were modeled, and ages and magnitudes of adiposity peak and rebound were estimated. Associations between infant feeding practices and growth were investigated by multivariable linear regression in children after testing a child-sex interaction.ResultsIn the studied population (n=1 225), adiposity peak occurred at a mean of 9.9 ± 2 months and adiposity rebound at 5.5 ± 1.4 years. Associations between infant feeding practices and adiposity peak or rebound were moderated by child sex. For girls, each additional month of breastfeeding was related to a 2-day increase in the age at adiposity peak (p
Published: 2022
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7. Maternal Diet Quality during Pregnancy and Allergic and Respiratory Multimorbidity Clusters in Children from the EDEN Mother–Child Cohort

Author: Rosalie Delvert, Manel Ghozal, Karine Adel-Patient, Manik Kadawathagedara, Barbara Heude, Marie-Aline Charles, Isabella Annesi-Maesano, Muriel Tafflet, Bénédicte Leynaert, Raphaëlle Varraso, Blandine de Lauzon-Guillain, Annabelle Bédard, Centre de recherche en épidémiologie et santé des populations (CESP), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, Université Paris Cité (UPCité), Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Paris-Saclay, CEA- Saclay (CEA), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Etude longitudinale française depuis l'enfance (UMS : Ined-Inserm-EFS) (ELFE), Institut national d'études démographiques (INED)-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Desbrest de santé publique (IDESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Institut National de Prévention et d'Éducation pour la Santé, INPES, Agence Nationale de la Recherche, ANR: ANR-19-CE36-0008, Institut National de la Santé et de la Recherche Médicale, Inserm, Fondation pour la Recherche Médicale, FRM, Ministère des Affaires Sociales et de la Santé, Université Paris-Sud, Agence Française de Sécurité Sanitaire de l'Environnement et du Travail, AFSSET, Mutuelle Générale de l'Education Nationale, MGEN, Institut de Veille Sanitaire, InVS, Part of this study was included in the InfaDiet project, funded by a grant from the French National Research Agency (ANR-19-CE36-0008). The EDEN study is supported by Fondation pour la Recherche Médicale (FRM), French Ministry of Research: Federative Research Institutes and Cohort Program, INSERM Human Nutrition National Research Program, and Diabetes National Research Program (through a collaboration with the French Association of Diabetic Patients (AFD)), French Ministry of Health, French Agency for Environment Security (AFSSET), French National Institute for Population Health Surveillance (InVS), Paris-Sud University, French National Institute for Health Education (INPES), Nestlé, Mutuelle Générale de l’Education Nationale (MGEN), French-speaking Association for the Study of Diabetes and Metabolism (ALFEDIAM), National Agency for Research (ANR non-thematic programme), and National Institute for Research in Public Health (IRESP: TGIR 2008 cohort in health programme). The funders had no role in the study design, data collection and analysis, decision to publish or preparation of the manuscript., and ANR-19-CE36-0008,InfaDiet,Influence de l'alimentation infantile sur la croissance et le développement de l'enfant(2019)
Subjects: maternal diet, asthma and allergic diseases, children, cluster analysis, birth cohort, Nutrition and Dietetics, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Food Science
Abstract: We investigated the associations between maternal diet quality and allergic and respiratory diseases in children. Analyses were based on 1316 mother–child pairs from the EDEN mother–child cohort. Maternal diet quality during pregnancy was assessed through a food-based score (the Diet Quality), a nutrient-based score (the PANDiet), and the adherence to guidelines for main food groups. Clusters of allergic and respiratory multimorbidity clusters up to 8 years were identified using Latent Class Analysis. Associations were assessed by adjusted multinomial logistic regressions. Four clusters were identified for children: “asymptomatic” (67%, reference group), “asthma only” (14%), “allergies without asthma” (12%), “multi-allergic” (7%). These clusters were not associated with mother diet quality assessed by both scores. Children from mothers consuming legumes once a month or less were at higher risk of belonging to the “multi-allergic” cluster (odds ratio (OR) (95% confidence interval (95%CI)) = 1.60 (1.01;2.54)). No association was found with other food groups or other clusters. In our study, allergic and respiratory multimorbidity in children was described with four distinct clusters. Our results suggest an interest in legumes consumption in the prevention of allergic diseases but need to be confirmed in larger cohorts and randomized control trials.
Published: 2023
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8. Detecting Hospital Outliers in Post-Pancreatectomy Care Using Funnel Plots from 2009–2018 Based on Nationwide Medico-Administrative Data

Author: Anne-Sophie Mariet, Olivier Facy, Serge Aho, Alexandre Doussot, Catherine Quantin, Alain Bernard, Jonathan Cottenet, Service de chirurgie cardio-vasculaire et thoracique (CHU Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Service Biostatistiques et Informatique Médicale (CHU de Dijon) (DIM), Service de bactériologie et hygiène hospitalière [Nantes], Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes), Service de Chirurgie Digestive, Cancérologique, Générale, Endocrinienne et Urgences (CHU de Dijon), Centre d'Investigation Clinique 1432 (Dijon) - Epidemiologie Clinique/Essais Cliniques (CIC-EC), Université de Bourgogne (UB)-Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de recherche en épidémiologie et santé des populations (CESP), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, Institut National de la Santé et de la Recherche Médicale, Inserm, The authors would like to thank Suzanne Rankin (Dijon University Hospital) for revising the manuscript. This work was supported by the Multi-organization Cancer Institute (Institut th?matique multi-organismes cancer: ITMO Cancer), the Public Health Research Institute (Institut de Recherche en Sant? Publique: IRESP) and the French National Institute of Health and Medical Research (Institut national de la sant? et de la recherche m?dicale: INSERM) in the context of the 2014?2019 cancer plan., The authors would like to thank Suzanne Rankin (Dijon University Hospital) for revising the manuscript. This work was supported by the Multi-organization Cancer Institute (Institut thématique multi-organismes cancer: ITMO Cancer), the Public Health Research Institute (Institut de Recherche en Santé Publique: IRESP) and the French National Institute of Health and Medical Research (Institut national de la santé et de la recherche médicale: INSERM) in the context of the 2014–2019 cancer plan., and HAL UVSQ, Équipe
Subjects: medicine.medical_specialty, Funnel plot, Original Scientific Report, Databases, Factual, [SDV]Life Sciences [q-bio], medicine.medical_treatment, Hospital quality, Pancreatic surgery, 03 medical and health sciences, Pancreatectomy, 0302 clinical medicine, medicine, Humans, 030212 general & internal medicine, business.industry, General surgery, Mortality rate, Hospitals, 3. Good health, [SDV] Life Sciences [q-bio], Cardiothoracic surgery, Mortality data, 030220 oncology & carcinogenesis, Surgery, National database, France, business
Abstract: Objectives Our objective was to identify hospitals with unusual mortality rates for major pancreatectomies over a period of ten years using 30-day mortality data from the French national database. Methods Data for all patients who underwent pancreatectomy were extracted from the national medico-economic database (Programme de Médicalisation des Systèmes d'Information). To identify quality outliers for each hospital, the observed-to-expected 30-day mortality rates were used as a quality indicator. Results A total of 19 494 patients underwent a major pancreatectomy in France between January 2009 and December 2018. The overall 30-day mortality rate was 4.8% (n = 944). For the 2009–2014 period, the funnel plot showed that 10 of the 176 hospitals lie outside the central 95% region and 7 lie outside the central 99.8% region. For the 2015–2018 period, out of 176 hospitals, 6 lie outside the central 95% region and 2 lie outside the central 99.8% region. The change in standardized mortality ratios between 2009–2014 and 2015–2018 testing for differences from the overall change, they were there 4 hospitals lie outside the central 95% region and 0 lie outside the central 99.8% region. Conclusion Over time, the improvement in hospital quality was weak. This study suggests that there is a pressing need to reorganize the supply of care for pancreatic surgery in France.
Published: 2021
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9. Maternal blood pressure associates with placental DNA methylation both directly and through alterations in cell-type composition

Author: Lucile Broséus, Daniel Vaiman, Jörg Tost, Camino Ruano San Martin, Milan Jacobi, Joel D. Schwartz, Rémi Béranger, Rémy Slama, Barbara Heude, Johanna Lepeule, Chard-Hutchinson, Xavier, APPEL À PROJETS GÉNÉRIQUE 2018 - Exposition prénatale au tabac et à la pollution atmosphérique et effets sur la santé respiratoire et le neurodévelopment de l'enfant: rôle de la méthylation placentaire - - ETAPE2018 - ANR-18-CE36-0005 - AAPG2018 - VALID, Contaminants et Environnements : Santé, Adaptabilité, Comportements et Usages - Effets de la Pollution Atmosphérique sur la fonction Placentaire et le développement Post-natal - - EPPAP2013 - ANR-13-CESA-0011 - CESA - VALID, Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Harvard T.H. Chan School of Public Health, École des Hautes Études en Santé Publique [EHESP] (EHESP), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), French National Cancer Institute (INCa), French Institute for Public Health Research (IreSP) [INCa_13641], Fondation de France [2012-00031593, 2012-00031617], European Union, Diabetes National Research Program (French Association of Diabetic Patients (AFD)), ANSES, Mutuelle Generale de l'Education Nationale (MGEN), French National Agency for Food Security, Frenchspeaking Association for the Study of Diabetes and Metabolism (ALFEDIAM), Foundation for Medical Research (FRM), National Institute for Research in Public Health (IRESP: TGIR cohorte sante 2008 program), French Ministry of Health (DGS), French Ministry of Research, Inserm Bone and Joint Diseases National Research (PRO-A), Human Nutrition National Research Programs, Paris-Sud University, Nestle, French National Institute for Population Health Surveillance (InVS), French National Institute for Health Education (INPES), ANR-18-CE36-0005,ETAPE,Exposition prénatale au tabac et à la pollution atmosphérique et effets sur la santé respiratoire et le neurodévelopment de l'enfant: rôle de la méthylation placentaire(2018), and ANR-13-CESA-0011,EPPAP,Effets de la Pollution Atmosphérique sur la fonction Placentaire et le développement Post-natal(2013)
Subjects: [SDV]Life Sciences [q-bio], Placenta, Mesenchymal stromal cells, Blood Pressure, General Medicine, DNA Methylation, Cell-type heterogeneity, Epigenesis, Genetic, [SDV] Life Sciences [q-bio], Cohort Studies, Epigenome-wide association study, Pregnancy, Hypertension, Humans, Female, CpG Islands, Child
Abstract: Background Maternal blood pressure levels reflect cardiovascular adaptation to pregnancy and proper maternal-fetal exchanges through the placenta and are very sensitive to numerous environmental stressors. Maternal hypertension during pregnancy has been associated with impaired placental functions and with an increased risk for children to suffer from cardiovascular and respiratory diseases later on. Investigating changes in placental DNA methylation levels and cell-type composition in association with maternal blood pressure could help elucidate its relationships with placental and fetal development. Methods Taking advantage of a large cohort of 666 participants, we investigated the association between epigenome-wide DNA methylation patterns in the placenta, measured using the Infinium HumanMethylation450 BeadChip, placental cell-type composition, estimated in silico, and repeated measurements of maternal steady and pulsatile blood pressure indicators during pregnancy. Results At the site-specific level, no significant association was found between maternal blood pressure and DNA methylation levels after correction for multiple testing (false discovery rate < 0.05), but 5 out of 24 previously found CpG associations were replicated (p-value < 0.05). At the regional level, our analyses highlighted 64 differentially methylated regions significantly associated with at least one blood pressure component, including 35 regions associated with mean arterial pressure levels during late pregnancy. These regions were found enriched for genes implicated in lung development and diseases. Further mediation analyses show that a significant part of the association between steady blood pressure—but not pulsatile pressure—and placental methylation can be explained by alterations in placental cell-type composition. In particular, elevated blood pressure levels are associated with a decrease in the ratio between mesenchymal stromal cells and syncytiotrophoblasts, even in the absence of preeclampsia. Conclusions This study provides the first evidence that the association between maternal steady blood pressure during pregnancy and placental DNA methylation is both direct and partly explained by changes in cell-type composition. These results could hint at molecular mechanisms linking maternal hypertension to lung development and early origins of childhood respiratory problems and at the importance of controlling maternal blood pressure during pregnancy.
Published: 2022
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10. Stability of ex situ biological methanation of H2/CO2 with a mixed microbial culture in a pilot scale bubble column reactor

Author: Léa Laguillaumie, Rafrafi Yan, Moya-Leclair Elisabeth, Delagnes Delphine, Dubos Simon, Spérandio Mathieu, Paul Etienne, Claire Dumas, Toulouse Biotechnology Institute (TBI), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), French National Institute of Applied Sciences (INSA) of Toulouse, French National Institute for Agricultural Research (INRAE), French agency for ecological transition (ADEME), Région Occitanie, and Bioenergies, Biomolecules, Biomaterials, renewable carbon recovery (3BCAR)
Subjects: History, Environmental Engineering, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Polymers and Plastics, CO 2 utilisation, Renewable Energy, Sustainability and the Environment, methane, gases fermentation, Bioengineering, General Medicine, Industrial and Manufacturing Engineering, homoacetogenesis, chemolithoautotrophs, microbial competition, CO2 utilisation, propionate, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, Business and International Management, acetate, Waste Management and Disposal, biotechnology
Abstract: International audience; Biological methanation is a promising technology for gas and carbon valorisation. Therefore, process stability is required to allow its scale up and development. A pilot scale bubble column reactor was used for ex situ biological methanation with Mixed Microbial Culture (MMC). A 16S rRNA high throughput sequencing analysis revealed the MMC reached a stable composition with 50-60% Methanobacterium in closed liquid mode, a robust genus adapted to large scale constraints. Class MBA03 was identified as an indicator of process stability. Methanogenic genera moved toward 50% of Methanothermobacter when intensifying the process, and proteolytic activity was identified while 94% of H2/CO2 was converted into methane at 4NL.L-1.d-1. This study gives clarifications on the origin of volatile fatty acids (VFA) apparitions. Acetate and propionate accumulated when methanogenic activity weakened due to nutritive deficiency, and when PH2 reached 0.7 bar. The MMC withstood a storage period of 34d at room temperature indicating its suitability for industrial constraints.
Published: 2022
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11. Family-focused contextual factors associated with lifestyle patterns in young children from two mother-offspring cohorts: GUSTO and EDEN

Author: Airu Chia, Alexandra Descarpentrie, Rene N. Cheong, Jia Ying Toh, Padmapriya Natarajan, Ray Sugianto, Shirong Cai, Cécilia Saldanha-Gomes, Patricia Dargent-Molina, Blandine de Lauzon-Guillain, Sabine Plancoulaine, Carla Lança, Seang Mei Saw, Keith M. Godfrey, Lynette P. Shek, Kok Hian Tan, Marie-Aline Charles, Yap Seng Chong, Barbara Heude, Johan G. Eriksson, Falk Müller-Riemenschneider, Sandrine Lioret, Mary F.-F. Chong, Jonathan Y. Bernard, Clinicum, Research Programs Unit, Johan Eriksson / Principal Investigator, Department of General Practice and Primary Health Care, Saw Swee Hock School of Public Health [Singapore, Singapore], National University of Singapore (NUS), Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université (HESAM)-HESAM Université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Singapore Institute for Clinical Sciences, Agency for science, technology and research [Singapore] (A*STAR), Singapore Institute for Clinical Sciences [Singapour] (SICS), Singapore Eye Research Institute [Singapore] (SERI), NIHR Southampton Biomedical Research Centre, Yong Loo Lin School of Medicine [Singapore], KK Women's and Children's Hospital [Singapore], This research is supported by the Paris‐NUS grant (ANR‐18‐IDEX‐0001).The EDEN study is supported by Foundation for Medical Research (FRM), National Agency for Research (ANR), National Institute for Research in Public Health (IRESP: TGIR cohorte santé 2008 program), French Ministry of Health (DGS), French Ministry of Research, INSERM Bone and Joint Diseases National Research (PRO‐A), and Human Nutrition National Research Programs, Paris‐Sud University, Nestlé, French National Institute for Population Health Surveillance (InVS.), French National Institute for Health Education (INPES), the European Union FP7 programmes (FP7/2007–2013, HELIX, ESCAPE, ENRIECO, Medall projects), Diabetes National Research Program (through a collaborationwith the French Association of Diabetic Patients (AFD)), French Agency for Environmental Health Safety (now ANSES), Mutuelle Générale de l’Education Nationale a complementary health insurance (MGEN), French National Agency for Food Security, French‐speaking Association for the Study of Diabetes and Metabolism (ALFEDIAM).The GUSTO study is supported by the Singapore National Research Founda‐ tion under its Translational and Clinical Research Flagship Programme and administered by the Singapore Ministry of Health’s National Medical Research Council, Singapore ‐ NMRC/TCR/004‐NUS/2008, NMRC/TCR/012‐NUHS/2014. Additional funding is provided by the Singapore Institute for Clinical Sciences, Agency for Science Technology and Research, Singapore. KMG is supported by the UK Medical Research Council (MC_UU_12011/4), the National Institute for Health Research (NIHR Senior Investigator (NF‐SI‐0515‐10042) and NIHR South‐ ampton Biomedical Research Centre (IS‐BRC‐1215‐20004)), the European Union (Erasmus+ Programme ImpENSA 598488‐EPP‐1‐2018‐1‐DE‐EPPKA2‐ CBHE‐JP) and the British Heart Foundation (RG/15/17/3174). The funders had no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript., and ANR-18-IDEX-0001,Université de Paris,Université de Paris(2018)
Subjects: Male, QUESTIONNAIRE, Mothers, Medicine (miscellaneous), Physical Therapy, Sports Therapy and Rehabilitation, Pregnancy, FOOD, Humans, Hierarchical analysis, VALIDITY, Child, Life Style, Nutrition and Dietetics, Physical activity, Preschool children, 1184 Genetics, developmental biology, physiology, Feeding Behavior, ADULTS, Screen time, Diet, DIETARY PATTERNS, PHYSICAL-ACTIVITY, Lifestyle patterns, Child, Preschool, OBESITY, 3121 General medicine, internal medicine and other clinical medicine, Female, Television, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Family ecological model, Snacks, 3143 Nutrition, TRANSITION
Abstract: Background Integrated patterns of energy balance-related behaviours of preschool children in Asia are sparse, with few comparative analyses. Purpose Using cohorts in Singapore (GUSTO) and France (EDEN), we characterized lifestyle patterns of children and investigated their associations with family-focused contextual factors. Methods Ten behavioural variables related to child’s diet, walking, outdoor play and screen time were ascertained by parental questionnaires at age 5–6 years. Using principal component analysis, sex-specific lifestyle patterns were derived independently for 630 GUSTO and 989 EDEN children. Contextual variables were organised into distal (family socio-economics, demographics), intermediate (parental health, lifestyle habits) and proximal (parent-child interaction factors) levels of influence and analysed with hierarchical linear regression. Results Three broadly similar lifestyle patterns were identified in both cohorts: “discretionary consumption and high screen time”, “fruit, vegetables, and low screen time” and “high outdoor playtime and walking”. The latter two patterns showed small differences between cohorts and sexes. The “discretionary consumption and high screen time” pattern was consistently similar in both cohorts; distal associated factors were lower maternal education (EDEN boys), no younger siblings (GUSTO boys) and Malay/Indian ethnicity (GUSTO), while intermediate and proximal associated factors in both cohorts and sexes were poor maternal diets during pregnancy, parents allowing high child control over food intake, snacking between meals and having television on while eating. Conclusions Three similar lifestyle patterns were observed among preschool children in Singapore and France. There were more common associated proximal factors than distal ones. Cohort specific family-focused contextual factors likely reflect differences in social and cultural settings. Findings will aid development of strategies to improve child health.
Published: 2022
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12. Urban environment and cognitive and motor function in children from four European birth cohorts

Author: Marina Vafeiadi, Raquel Soler-Blasco, Mariza Kampouri, Mònica Guxens, Martine Vrijheid, Johanna Lepeule, Llúcia González-Safont, Mark J. Nieuwenhuijsen, Mark Mon-Williams, John Wright, Rosie McEachan, Anne-Claire Binter, Claire Philippat, Jonathan Y. Bernard, Jordi Sunyer, Loreto Santa-Marina, Leda Chatzi, Lucia Alonso, Ainara Andiarena, Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Instituto de Salud Global - Institute For Global Health [Barcelona] (ISGlobal), Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Agency for science, technology and research [Singapore] (A*STAR), University of South-Eastern Norway (USN), University of the Basque Country/Euskal Herriko Unibertsitatea (UPV/EHU), CIBER de Epidemiología y Salud Pública (CIBERESP), Universitat Jaume I, University of Crete [Heraklion] (UOC), Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana [Espagne] (FISABIO), University of Bradford, University of Southern California (USC), Universitat Pompeu Fabra [Barcelona] (UPF), Erasmus University Medical Center [Rotterdam] (Erasmus MC), This work was supported by funding from the European Community’s Seventh Framework Programme [FP7/2007–206 n◦308333, the HELIX project]. This INMA cohort was funded by grants from Instituto de Salud Carlos III (Red INMA G03/176, CB06/02/0041, PI041436, PI081151 incl. FEDER funds, FIS PI06/0867, FIS-PI09/00090, FIS and FIS-PI18/01142 incl. FEDER funds, FIS-FEDER: PI03/1615, PI04/1509, PI04/1112, PI04/1931, PI05/1079, PI05/1052, PI06/1213, PI07/0314, PI09/02647, PI11/01007, PI11/02591, PI11/02038, PI13/1944, PI13/2032, PI14/00891, PI14/01687, PI16/1288, PI16/00118 and PI17/00663, FIS-FSE: 17/00260, Miguel Servet-FEDER CP11/00178, CP15/00025, CPII16/00051, and CPII18/00018), from UE (FP7-ENV-2011 cod 282957, HEALTH.2010.2.4.5-1, and H2020 n◦824989), Generalitat de Catalunya-CIRIT 1999SGR 00241, Fundació La marató de TV3 (090430), Generalitat Valenciana: FISABIO (UGP 15-230, UGP-15-244, and UGP-15-249), Alicia Koplowitz Foundation 2017, CIBERESP, Department of Health of the Basque Government (2013111089, 2009111069, 2013111089, 2015111065 and 2018111086), Provincial Government of Gipuzkoa (DFG06/002, DFG08/001, DFG15/221 and DFG 89/17) and annual agreements with the municipalities of the study area (Zumarraga, Urretxu , Legazpi, Azkoitia y Azpeitia y Beasain). We acknowledge support from the Spanish Ministry of Science and Innovation and the State Research Agency through the 'Centro de Excelencia Severo Ochoa 2019-2023' Program (CEX2018-000806-S), and support from the Generalitat de Catalunya through the CERCA Program. The work was also supported by MICINN [MTM2015-68140- R] and Centro Nacional de Genotipado- CEGEN- PRB2- ISCIII (Spain). The Rhea project was financially supported by European projects, and the Greek Ministry of Health (Program of Prevention of obesity and neurodevelopmental disorders in preschool children, in Heraklion district, Crete, Greece: 2011–2014, 'Rhea Plus': Primary Prevention Program of Environmental Risk Factors for Reproductive Health, and Child Health: 2012–15). This paper presents independent research funded by the National Institute for Health Research (NIHR) under its Collaboration for Applied Health Research and Care (CLAHRC) for Yorkshire and Humber (UK). The EDEN study was supported by Foundation for medical research (FRM), National Agency for Research (ANR), National Institute for Research in Public health (IRESP: TGIR cohorte santé 2008 program), French Ministry of Health (DGS), French Ministry of Research, INSERM Bone and Joint Diseases National Research (PRO-A), and Human Nutrition National Research Programs, Paris-Sud University, Nestlé, French National Institute for Population Health Surveillance (InVS), French National Institute for Health Education (INPES), the European Union FP7 programs (ESCAPE, ENRIECO, Medall projects), Diabetes National Research Program (through a collaboration with the French Association of Diabetic Patients (AFD)), French Agency for Environmental Health Safety and French National Agency for Food Security (now ANSES), Mutuelle Générale de l’Education Nationale a complementary health insurance (MGEN), French-speaking association for the study of diabetes and metabolism (ALFEDIAM). Core support for Born in Bradford is also provided by the Wellcome Trust (WT101597MA, UK). Born in Bradford (BiB) is only possible because of the enthusiasm and commitment of the children and parents in BiB. We are grateful to all the participants, health professionals, schools and researchers who have made BiB happen. BiB receives funding from the ESRC/MRC, the Wellcome Trust (WT101597MA) and the National Institute for Health Research Yorkshire and Humber ARC (reference: NIHR20016). M. Mon-Williams was supported by a Fellowship from the Alan Turing Institute. Additional funding from the National Institute of Environmental Health Science supported Dr Chatzi (R01ES030691, R01ES029944, R01ES030364, R21ES029681, and P30ES007048). The views expressed are those of the authors, and not necessarily those of the NHS or the NIHR. None of the funders were involved in designing the study, collecting the data, analyzing or interpreting the data, deciding to submit the article for publication, or the writing of the report., HESAM Université (HESAM)-HESAM Université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Singapore Institute for Clinical Sciences [Singapour] (SICS), Bradford Institute for Health Research [Bradford, UK], Bradford Teaching Hospitals NHS Foundation Trust [Bradford, UK] (BTHFT), University of Leeds, Universitat de València (UV), Karolinska Institutet [Stockholm], Binter, Anne-Claire, Child and Adolescent Psychiatry / Psychology, Erasmus MC other, European Commission, Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Santé et de la Recherche Médicale (INSERM)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Université de Rennes 1 (UR1), and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Université d'Angers (UA)
Subjects: Urban environment, 010501 environmental sciences, 01 natural sciences, 0302 clinical medicine, Cognition, Pregnancy, Cognició en els infants, 11. Sustainability, GE1-350, 030212 general & internal medicine, Children, Motor skill, ComputingMilieux_MISCELLANEOUS, Desenvolupament urbà, General Environmental Science, SDG 15 - Life on Land, 2. Zero hunger, education.field_of_study, Air Pollutants, 4. Education, motor function, Cohort, cohort, SDG 11 - Sustainable Cities and Communities, 3. Good health, Child, Preschool, Birth Cohort, Female, Cognitive function, Psychology, Population, Gross motor skill, 03 medical and health sciences, children, Urban planning, Air Pollution, medicine, Humans, education, Motor function, cognitive function, 0105 earth and related environmental sciences, Environmental Exposure, medicine.disease, urban environment, Confidence interval, Environmental sciences, Spain, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, Particulate Matter, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Demography
Abstract: [EN]Background: The urban environment may influence neurodevelopment from conception onwards, but there is no evaluation of the impact of multiple groups of exposures simultaneously. We investigated the association between early-life urban environment and cognitive and motor function in children. Methods: We used data from 5403 mother-child pairs from four population-based birth-cohorts (UK, France, Spain, and Greece). We estimated thirteen urban home exposures during pregnancy and childhood, including: built environment, natural spaces, and air pollution. Verbal, non-verbal, gross motor, and fine motor functions were assessed using validated tests at five years old. We ran adjusted multi-exposure models using the Deletion-Substitution-Addition algorithm. Results: Higher greenness exposure within 300 m during pregnancy was associated with higher verbal abilities (1.5 points (95% confidence interval 0.4, 2.7) per 0.20 unit increase in greenness). Higher connectivity density within 100 m and land use diversity during pregnancy were related to lower verbal abilities. Childhood exposure to PM2.5 mediated 74% of the association between greenness during childhood and verbal abilities. Higher exposure to PM2.5 during pregnancy was related to lower fine motor function (-1.2 points (-2.1, -0.4) per 3.2 mu g/m3 increase in PM2.5). No associations were found with non-verbal abilities and gross motor function. Discussion: This study suggests that built environment, greenness, and air pollution may impact child cognitive and motor function at five years old. This study adds evidence that well-designed urban planning may benefit children's cognitive and motor development. Acknowledgements We are grateful to all the participating children, parents, practi-tioners and researchers in the four countries who took part in this study. This work was supported by funding from the European Community's Seventh Framework Programme [FP7/2007-206 n 308333; the HELIX project] . This INMA cohort was funded by grants from Instituto de Salud Carlos III (Red INMA G03/176; CB06/02/0041; PI041436; PI081151 incl. FEDER funds, FIS PI06/0867, FIS-PI09/00090, FIS and FIS-PI18/01142 incl. FEDER funds, FIS-FEDER: PI03/1615, PI04/1509, PI04/1112, PI04/1931, PI05/1079, PI05/1052, PI06/1213, PI07/0314, PI09/02647, PI11/01007, PI11/02591, PI11/02038, PI13/1944, PI13/2032, PI14/00891, PI14/01687, PI16/1288, PI16/00118 and PI17/00663; FIS-FSE: 17/00260; Miguel Servet-FEDER CP11/00178, CP15/00025, CPII16/00051, and CPII18/00018) , from UE (FP7-ENV-2011 cod 282957, HEALTH.2010.2.4.5-1, and H2020 n 824989) , Generalitat de Catalunya-CIRIT 1999SGR 00241, Fundacio La marato de TV3 (090430) , Generalitat Valenciana: FISABIO (UGP 15-230, UGP-15-244, and UGP-15-249) , Alicia Koplowitz Foundation 2017, CIBERESP, Department of Health of the Basque Government (2013111089, 2009111069, 2013111089, 2015111065 and 2018111086) , Provincial Government of Gipuzkoa (DFG06/002, DFG08/001, DFG15/221 and DFG 89/17) and annual agreements with the municipalities of the study area (Zumarraga, Urretxu , Legazpi, Azkoitia y Azpeitia y Beasain) . We acknowledge support from the Spanish Ministry of Science and Inno-vation and the State Research Agency through the "Centro de Excelencia Severo Ochoa 2019-2023" Program (CEX2018-000806-S) , and support from the Generalitat de Catalunya through the CERCA Program. The work was also supported by MICINN [MTM2015-68140-R] and Centro Nacional de Genotipado-CEGEN-PRB2-ISCIII (Spain) . The Rhea project was financially supported by European projects, and the Greek Ministry of Health (Program of Prevention of obesity and neurodevelopmental disorders in preschool children, in Heraklion district, Crete, Greece: 2011-2014; "Rhea Plus": Primary Prevention Program of Environmental Risk Factors for Reproductive Health, and Child Health: 2012-15) . This paper presents independent research funded by the National Institute for Health Research (NIHR) under its Collaboration for Applied Health Research and Care (CLAHRC) for Yorkshire and Humber (UK) . The EDEN study was supported by Foundation for medical research (FRM) , National Agency for Research (ANR) , National Institute for Research inPublic health (IRESP: TGIR cohorte sante 2008 program) , French Min-istry of Health (DGS) , French Ministry of Research, INSERM Bone and Joint Diseases National Research (PRO-A) , and Human Nutrition Na-tional Research Programs, Paris-Sud University, Nestle, French National Institute for Population Health Surveillance (InVS) , French National Institute for Health Education (INPES) , the European Union FP7 pro-grams (ESCAPE, ENRIECO, Medall projects) , Diabetes National Research Program (through a collaboration with the French Association of Diabetic Patients (AFD) ) , French Agency for Environmental Health Safety and French National Agency for Food Security (now ANSES) , Mutuelle Generale de l'Education Nationale a complementary health insurance (MGEN) , French-speaking association for the study of diabetes and metabolism (ALFEDIAM) . Core support for Born in Bradford is also provided by the Wellcome Trust (WT101597MA, UK) . Born in Bradford (BiB) is only possible because of the enthusiasm and commitment of the children and parents in BiB. We are grateful to all the participants, health professionals, schools and researchers who have made BiB happen. BiB receives funding from the ESRC/MRC, the Wellcome Trust (WT101597MA) and the National Institute for Health Research York-shire and Humber ARC (reference: NIHR20016) . M. Mon-Williams was supported by a Fellowship from the Alan Turing Institute. Additional funding from the National Institute of Environmental Health Science supported Dr Chatzi (R01ES030691, R01ES029944, R01ES030364, R21ES029681, and P30ES007048) . The views expressed are those of the authors, and not necessarily those of the NHS or the NIHR. None of the funders were involved in designing the study, collecting the data, analyzing or interpreting the data, deciding to submit the article for publication, or the writing of the report. Data sharing statement The HELIX data warehouse has been established as an accessible resource for collaborative research involving researchers external to the project. Access to HELIX data is based on approval by the HELIX Project Executive Committee and by the individual cohorts. Further details on the content of the data warehouse (data catalogue) and procedures for external access are described on the project website (http:// www.proj-ecthelix.eu/index.php/es/data-inventory) .
Published: 2022
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13. Association between dietary patterns reflecting one-carbon metabolism nutrients intake before pregnancy and placental DNA methylation

Author: Anne Forhan, Matthew Suderman, Blandine de Lauzon-Guillain, Marie-Aline Charles, Marion Lecorguillé, Johanna Lepeule, Jörg Tost, Barbara Heude, Sandrine Lioret, Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), University of Bristol [Bristol], The EDEN study was supported by Foundation for Medical Research (FRM), National Agency for Research (ANR), National Institute for Research in Public health (IRESP: TGIR cohorte santé 2008 program), French Ministry of Health (DGS), French Ministry of Research, INSERM Bone and Joint Diseases National Research (PRO-A), and Human Nutrition National Research Programs, Paris-Sud University, Nestlé, French National Institute for Population Health Surveillance (InVS), French National Institute for Health Education (INPES), the European Union FP7 programs (FP7/2007–2013, HELIX, ESCAPE, ENRIECO, Medall projects), Diabetes National Research Program (through a collaboration with the French Association of Diabetic Patients), French Agency for Environmental Health Safety (now ANSES), Mutuelle Générale de l’Education Nationale, a complementary health insurance, French national agency for food security, and French-speaking association for the study of diabetes and metabolism (ALFEDIAM). The placental DNA measurements were funded by the Fondation de France (grant 2012-00031617 - 2012-00031593) and the ANR (ANR-13-CESA-0011-05). The scientific mobility of M Lecorguillé into the MRC Integrative Epidemiology Unit for a 4-month research stay was possible in part because of funding from the ALPHABET project. This latter project was supported by an award from the European Union’s Horizon 2020 research and innovation program under the ERA-Net Cofund of the Joint Programming Initiative Healthy Diet for Healthy Life (JPI-HDHL) (http://www.healthydietforhealthylife.eu) (action no. 696295, Biomarkers for Nutrition and Health). M Lecorguillé received a doctoral mobility grant from the Public Health Doctoral Network from the EHESP (School for Higher Studies in Public Health) and the University of Paris Descartes., Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Lecorguillé, Marion
Subjects: Cancer Research, medicine.medical_specialty, Placenta, [SDV]Life Sciences [q-bio], dietary patterns, Biology, methyl-donors, global DNA methylation, Epigenesis, Genetic, 03 medical and health sciences, chemistry.chemical_compound, Eating, pre-conception period, 0302 clinical medicine, Betaine, Nutrient, Pregnancy, Internal medicine, medicine, Choline, Humans, maternal diet, Epigenetics, Micronutrients, placental DNA methylation, Molecular Biology, 030304 developmental biology, 2. Zero hunger, 0303 health sciences, Methionine, epigenetics, birth cohort, Nutrients, DNA Methylation, Micronutrient, epigenome-wide association study, Carbon, [SDV] Life Sciences [q-bio], B vitamins, Endocrinology, chemistry, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, 030220 oncology & carcinogenesis, DNA methylation, Female, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Research Paper
Abstract: International audience; Background: The preconception period represents an important window for fetal and epigenetic programming. Some micronutrients (B vitamins, choline, betaine, methionine) implicated in one-carbon metabolism (OCM) are essential for major epigenetic processes that take place in early pregnancy. However, few studies have evaluated the implication of the micronutrients in placental DNA methylation.Objective: We investigated whether intake of OCM nutrients in the year before pregnancy was associated with placental DNA methylation in the EDEN mother–child cohort.Design: Maternal dietary intake was assessed with a food-frequency questionnaire. Three dietary patterns, “varied and balanced diet”, “vegetarian tendency”, and “bread and starchy food”, were used to characterize maternal OCM dietary intake. The Illumina Infinium HumanMethylation450 BeadChip was used to measure placental DNA methylation of 573 women included in the analyses. We evaluated the association of dietary patterns with global DNA methylation. Then, we conducted an agnostic epigenome-wide association study (EWAS) and investigated differentially methylated regions (DMRs) associated with each dietary pattern. Results: We found no significant association between the three dietary patterns and global DNA methylation or individual CpG sites. DMR analyses highlighted associations between the “varied and balanced” or “vegetarian tendency” pattern and DMRs located at genes previously implicated in functions essential for embryonic development, such as neurodevelopment. The “bread and starchy food” pattern was associated with regions related to genes whose functions involve various metabolic and cell synthesis-related processes.Conclusions: In mainly well-nourished French women without major deficiencies, OCM intake before pregnancy was not associated with major variation in DNA methylation.
Published: 2022
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14. Short- and medium-term air pollution exposure, plasmatic protein levels and blood pressure in children

Author: Paula de Prado-Bert, Charline Warembourg, Audrius Dedele, Barbara Heude, Eva Borràs, Eduard Sabidó, Gunn Marit Aasvang, Johanna Lepeule, John Wright, Jose Urquiza, Kristine B. Gützkow, Léa Maitre, Leda Chatzi, Maribel Casas, Marina Vafeiadi, Mark J. Nieuwenhuijsen, Montserrat de Castro, Regina Grazuleviciene, Rosemary R.C. McEachan, Xavier Basagaña, Martine Vrijheid, Jordi Sunyer, Mariona Bustamante, Instituto de Salud Global - Institute For Global Health [Barcelona] (ISGlobal), CIBER de Epidemiología y Salud Pública (CIBERESP), Universitat Pompeu Fabra [Barcelona] (UPF), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), École des Hautes Études en Santé Publique [EHESP] (EHESP), Vytautas Magnus University - Vytauto Didziojo Universitetas (VDU), Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Barcelona Institute of Science and Technology (BIST), Norwegian Institute of Public Health [Oslo] (NIPH), Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), University of Bradford, University of Crete [Heraklion] (UOC), Keck School of Medicine [Los Angeles], University of Southern California (USC), The study received funding from the European Community's Seventh Framework Programme (FP7/2007–206) (grant agreement no 308333) (HELIX project), the H2020-EU.3.1.2. - Preventing Disease Programme (grant agreement no 874583) (ATHLETE project) and from the European Union’s Horizon 2020 research and innovation programme (grant Agreement number: 733206) (Early Life stressors and Lifecycle Health (LIFECYCLE)). BiB received funding from the Welcome Trust (WT101597MA), from the UK Medical Research Council (MRC) and Economic and Social Science Research Council (ESRC) (MR/N024397/1), and the National Institute for Health Research (NIHR) Applied Research Collaboration for Yorkshire and Humber. The EDEN study was supported by Foundation for medical research (FRM), National Agency for Research (ANR), National Institute for Research in Public health (IRESP: TGIR cohorte santé 2008 program), French Ministry of Health (DGS), French Ministry of Research, INSERM Bone and Joint Diseases National Research (PRO-A), and Human Nutrition National Research Programs, Paris-Sud University, Nestlé, French National Institute for Population Health Surveillance (InVS), French National Institute for Health Education (INPES), the European Union FP7 programmes (FP7/2007–2013, HELIX, ESCAPE, ENRIECO, Medall projects), Diabetes National Research Program (through a collaboration with the French Association of Diabetic Patients (AFD)), French Agency for Environmental Health Safety (now ANSES), Mutuelle Générale de l’Education Nationale a complementary health insurance (MGEN), French national agency for food security, French-speaking association for the study of diabetes and metabolism (ALFEDIAM). INMA was supported by grants from the Instituto de Salud Carlos III, CIBERESP, and the Generalitat de Catalunya-CIRIT. KANC was funded by the grant of the Lithuanian Agency for Science Innovation and Technology (6-04-2014_31V-66). The Norwegian Mother, Father and Child Cohort Study is supported by the Norwegian Ministry of Health and Care Services and the Ministry of Education and Research. The Rhea project was financially supported by European projects (EU FP6-2003-Food-3-NewGeneris, EU FP6. STREP Hiwate, EU FP7 ENV. 2007.1.2.2.2. Project No 211250 Escape, EU FP7-2008-ENV-1.2.1.4 Envirogenomarkers, EU FP7-HEALTH-2009- single stage CHICOS, EU FP7 ENV. 2008.1.2.1.6. Proposal No 226285 ENRIECO, EU- FP7- HEALTH-2012 Proposal No 308333 HELIX), and the Greek Ministry of Health (Program of Prevention of obesity and neurodevelopmental disorders in preschool children, in Heraklion district, Crete, Greece: 2011–2014, and 'Rhea Plus': Primary Prevention Program of Environmental Risk Factors for Reproductive Health, and Child Health: 2012–15). MC received funding from Instituto Carlos III (Ministry of Economy and Competitiveness) (CD12/00563 and MS16/00128). JU is supported by Spanish regional program PERIS (Ref.: SLT017/20/000119) Granted by Departament de Salut de la Generalitat de Catalunya. The CRG/UPF Proteomics Unit is part of the Spanish Infrastructure for Omics Technologies (ICTS OmicsTech) and it is member of the ProteoRed PRB3 consortium which is supported by grant PT17/0019 of the PE I + D + i 2013–2016 from the Instituto de Salud Carlos III (ISCIII). Before the start of HELIX, all six cohorts had undergone the required evaluation by national ethics committees and obtained all the required permissions for their cohort recruitment and follow-up visits. The work in HELIX was covered by new ethic approvals in each country and at enrolment in the new follow-up, participants were asked to sign a new informed consent form.
Subjects: Plasmatic proteins, Air Pollutants, Short-term effects, Aire -- Contaminació, [SDV]Life Sciences [q-bio], Nitrogen Dioxide, Blood Pressure, Environmental Exposure, Environment, Pressió sanguínia, Childhood, [SDE.ES]Environmental Sciences/Environmental and Society, Biochemistry, Air Pollution, Humans, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Particulate Matter, Child, Infants, Proteïnes, General Environmental Science
Abstract: Exposure to air pollution influences children's health, however, the biological mechanisms underlying these effects are not completely elucidated. We investigated the association between short- and medium-term outdoor air pollution exposure with protein profiles and their link with blood pressure in 1170 HELIX children aged 6-11 years. Different air pollutants (NO2, PM10, PM2.5, and PM2.5abs) were estimated based on residential and school addresses at three different windows of exposure (1-day, 1-week, and 1-year before clinical and molecular assessment). Thirty-six proteins, including adipokines, cytokines, or apolipoproteins, were measured in children's plasma using Luminex. Systolic and diastolic blood pressure (SBP and DBP) were measured following a standardized protocol. We performed an association study for each air pollutant at each location and time window and each outcome, adjusting for potential confounders. After correcting for multiple-testing, hepatocyte growth factor (HGF) and interleukin 8 (IL8) levels were positively associated with 1-week home exposure to some of the pollutants (NO2, PM10, or PM2.5). NO2 1-week home exposure was also related to higher SBP. The mediation study suggested that HGF could explain 19% of the short-term effect of NO2 on blood pressure, but other study designs are needed to prove the causal directionality between HGF and blood pressure. The study received funding from the European Community’s Seventh Framework Programme (FP7/2007–206) (grant agreement no 308333) (HELIX project), the H2020-EU.3.1.2. - Preventing Disease Programme (grant agreement no 874583) (ATHLETE project) and from the European Union’s Horizon 2020 research and innovation programme (grant Agreement number: 733206) (Early Life stressors and Lifecycle Health (LIFECYCLE)). BiB received funding from the Welcome Trust (WT101597MA), from the UK Medical Research Council (MRC) and Economic and Social Science Research Council (ESRC) (MR/N024397/ 1). The study was supported by the European Union FP7 programmes (FP7/ 2007–2013, HELIX, ESCAPE, ENRIECO, Medall projects). INMA was supported by grants from the Instituto de Salud Carlos III, CIBERESP, and the Generalitat de Catalunya-CIRIT. KANC was funded by the grant of the Lithuanian Agency for Science Innovation and Technology (6-04-2014_31V-66). The Rhea project was financially supported by European projects (EU FP6-2003-Food-3-NewGeneris, EU FP6. STREP Hiwate, EU FP7 ENV. 2007.1.2.2.2. Project No 211250 Escape, EU FP7- 2008-ENV-1.2.1.4 Envirogenomarkers, EU FP7-HEALTH-2009- single stage CHICOS, EU FP7 ENV. 2008.1.2.1.6. Proposal No 226285 ENRIECO, EU- FP7- HEALTH-2012 Proposal No 308333 HELIX). MC received funding from Instituto Carlos III (Ministry of Economy and Competitiveness) (CD12/00563 and MS16/00128). JU is supported by Spanish regional program PERIS (Ref.: SLT017/20/ 000119) Granted by Departament de Salut de la Generalitat de Catalunya. The CRG/UPF Proteomics Unit is part of the Spanish Infrastructure for Omics Technologies (ICTS OmicsTech) and it is member of the ProteoRed PRB3 consortium which is supported by grant PT17/0019 of the PE I + D + i 2013–2016 from the Instituto de Salud Carlos III (ISCIII)
Published: 2022
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15. Associations between maternal eating behaviors and feeding practices in toddlerhood

Author: Claire Guivarch, Marie-Aline Charles, Anne Forhan, Barbara Heude, Blandine de Lauzon-Guillain, Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Etude longitudinale française depuis l'enfance (UMS : Ined-Inserm-EFS) (ELFE), Institut national d'études démographiques (INED)-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), The EDEN study is supported by Fondation pour la Recherche Médicale (FRM), French Ministry of Research: Federative Research Institutes and Cohort Program, INSERM Human Nutrition National Research Program, and Diabetes National Research Program (through a collaboration with the French Association of Diabetic Patients [AFD]), French Ministry of Health, French Agency for Environment Security (AFSSET), French National Institute for Population Health Surveillance (InVS), Paris‐Sud University, French National Institute for Health Education (INPES), Nestlé, Mutuelle Générale de l'Education Nationale (MGEN), French-speaking Association for the Study of Diabetes and Metabolism (ALFEDIAM), National Agency for Research (ANR non‐thematic programme), and National Institute for Research in Public Health (IRESP: TGIR 2008 cohort in health programme)., and de Lauzon-Guillain, Blandine
Subjects: Male, Eating behaviors, Nutrition and Dietetics, toddlerhood, Parenting, Child Behavior, Mothers, birth cohort, feeding practices, Feeding Behavior, Body Mass Index, Eating, Cross-Sectional Studies, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, Surveys and Questionnaires, Humans, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Female, Child, Maternal Behavior, coercive practices, General Psychology
Abstract: International audience; Few studies have examined the associations between parents' own eating behaviors and their feeding practices. We aimed to study the associations between maternal eating behaviors and feeding practices in toddlerhood. In this cross-sectional analysis, maternal eating behaviors and feeding practices were assessed at 2-year follow-up by using the Three-Factor Eating Questionnaire (TFEQ-R21) and the Comprehensive Feeding Practices Questionnaire (CFPQ), respectively, among mothers of 1322 children from the EDEN mother-child cohort. Depending on their distributions, scores from the two questionnaires were considered continuous or binary variables, according to the median. Linear or logistic regression models were used as appropriate to assess the associations between maternal eating behaviors, considered simultaneously in a combined model, and their feeding practices. Maternal cognitive restraint was positively associated with maternal restriction for health and restriction for weight. Maternal uncontrolled eating was positively associated with pressure to eat and use of food to regulate the child's emotions. Maternal uncontrolled eating was also negatively associated with restriction for weight, but only among boys. This study supports that mothers' own eating behaviors are associated with their feeding practices in toddlerhood. Further studies are needed to understand the role of parental feeding practices in the familial transmission of eating behavior.
Published: 2022
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16. Clusters of diet, physical activity, television exposure and sleep habits and their association with adiposity in preschool children: the EDEN mother-child cohort

Author: SALDANHA-GOMES, Cécilia, Marbac, Matthieu, Sedki, Mohammed, Cornet, Maxime, Plancoulaine, Sabine, Charles, Marie-Aline, Lioret, Sandrine, Dargent-Molina, Patricia, Equipe 6 : ORCHAD - Origines précoces de la santé du développement de l'enfant (CRESS - U1153), Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Paris-Saclay, Centre de Recherche en Économie et Statistique (CREST), Ecole Nationale de la Statistique et de l'Analyse de l'Information [Bruz] (ENSAI)-École polytechnique (X)-École Nationale de la Statistique et de l'Administration Économique (ENSAE Paris)-Centre National de la Recherche Scientifique (CNRS), Centre de recherche en épidémiologie et santé des populations (CESP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris-Sud - Paris 11 (UP11)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), The EDEN mother cohort is funded by National Institute for Research in Public Health (IRESP TGIR Cohorte Santé 2008 Program), National Agency for Research (ANR nonthematic program), French Speaking Association for the Study of Diabetes and Metabolism (Alfediam), Mutuelle Générale de l’Éducation Nationale, Nestlé, French National Institute for Health Education (INPES), Paris-Sud University, French National Institute for Population Health Surveillance (INVS), French Agency for Environment Security (AFFSET), French Ministry of Health Perinatal Program, INSERM Nutrition Research Program, Institut Fédératif de Recherche and Cohort Program, French Ministry of Research, Fondation pour la Recherche Médicale., Université Paris Descartes - Paris 5 (UPD5)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Ecole Nationale de la Statistique et de l'Analyse de l'Information [Bruz] (ENSAI)-École polytechnique (X)-École Nationale de la Statistique et de l'Administration Économique (ENSAE ParisTech )-Centre National de la Recherche Scientifique (CNRS), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Sud - Paris 11 (UP11)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM), Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Sud - Paris 11 (UP11)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM), and SALDANHA-GOMES, Cécilia
Subjects: Male, Preschoolers, Physical activity, lcsh:Public aspects of medicine, Research, Mothers, lcsh:RA1-1270, Feeding Behavior, Lifestyle clusters, Overweight, Diet, lcsh:Nutritional diseases. Deficiency diseases, TV exposure, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, Child, Preschool, Latent class analysis, Humans, Female, Television, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Sleep, lcsh:RC620-627, Exercise, Adiposity
Abstract: International audience; Background: Despite the growing interest in the relation between adiposity in children and different lifestyle clusters, few studies used a longitudinal design to examine a large range of behaviors in various contexts, in particular eating-and sleep-related routines, and few studies have examined these factors in young children. The objectives of this study were to identify clusters of boys and girls based on diet, sleep and activity-related behaviors and their family environment at 2 and 5 years of age, and to assess whether the clusters identified varied across maternal education levels and were associated with body fat at age 5. Methods: At 2 and 5 years, respectively, 1436 and 1195 parents from the EDEN mother-child cohort completed a questionnaire including behavioral data. A latent class analysis aimed to uncover gender-specific behavioral clusters. Body fat percentage was estimated by anthropometric and bioelectrical impedance measurements. Association between cluster membership and body fat was assessed with mutivariable linear regression models. Results: At 2 years, two clusters emerged that were essentially characterized by opposite eating habits. At 5 years, TV exposure was the most distinguishing feature, but the numbers and types of clusters differed by gender. An association between cluster membership and body fat was found only in girls at 5 years of age, with girls in the cluster defined by very high TV exposure and unfavorable mealtime habits (despite high outdoor playing and walking time) having the highest body fat. Girls whose mother had low educational attainment were more likely to be in this high-risk cluster. Girls who were on a cluster evolution path corresponding to the highest TV viewing time and the least favorable mealtime habits from 2 to 5 years of age had higher body fat at 5 years. Conclusions: Efforts to decrease TV time and improve mealtime routines may hold promise for preventing overweight in young children, especially girls growing up in disadvantaged families. These preventive efforts should start as early in life as possible, ideally before the age of two, and should be sustained over the preschool years.
Published: 2020
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17. A simple method for high molecular-weight genomic DNA extraction suitable for long-read sequencing from spores of an obligate biotroph oomycete

Author: Penouilh-Suzette, Charlotte, Fourré, Sandra, Besnard, Guillaume, Godiard, Laurence, Pecrix, Yann, Laboratoire des Interactions Plantes Microbes Environnement (LIPME), Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Génome et Transcriptome - Plateforme Génomique ( GeT-PlaGe), Plateforme Génome & Transcriptome (GET), Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Evolution et Diversité Biologique (EDB), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Peuplements végétaux et bioagresseurs en milieu tropical (UMR PVBMT), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de La Réunion (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), This work was supported by the French ANR grant EFFECTOORES (ANR-13-ADAP-0003) and by the Inter-Units grant EVOPLASMO funded by the French Laboratory of Excellence project TULIP (LabexTULIP, ANR-10-LABX-41, ANR-11-IDEX-0002-02) and the Agrobiosciences, Interactions and Biodiversity Research Federation (FRAIB, FR3450). We thank the Sunflower Biological Resources Centre of the French National Institute for Agricultural Research (INRAE) forproviding sunflower seeds. We thank the Get-PlaGe platform for DNA measurements and sequencing, and the LIPM Bioinformatics platform for performing metrics calculations. GB is member of the EDB laboratory supported by the French Laboratory of Excellence project CEBA (LabexCEBA,ANR-10-LABX-25-01) and LabexTULIP, managed by theFrench ANR., Sunflower Biological Resources Centre of the French National Institute for Agricultural Research, ANR-13-ADAP-0003,EFFECTOORES,Exploitation des connaissances sur les effecteurs des Oomycetes pour la recherche de résistances durables aux maladies chez les plantes cultivées(2013), ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010), ANR-10-LABX-0025,CEBA,CEnter of the study of Biodiversity in Amazonia(2010), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), and Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Spores, PacBio, Oidium (manifestation maladies), [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics, High molecular weight genomic DNA, Agent pathogène, Spore fongique, Oomycete, Séquence d'ADN, Oomycetes, Long-read sequencing, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Plasmopara halstedii, H20 - Maladies des plantes, Downy mildew
Abstract: International audience; Long-read sequencing technologies are having a major impact on our approaches to studying non-model organisms and microbial communities. By significantly reducing the cost and facilitating the genome assembly pipelines, any laboratory can now develop its own genomics program regardless of the complexity of the genome studied. The most crucial current challenge is to develop efficient protocols for extracting genomic DNA (gDNA) with high quality and integrity adapted to the organism of interest. This can be particularly complex for obligate pathogens that must maintain intimate interactions inside infected host tissues. Here we propose a simple and cost-effective method for high molecular weight gDNA extraction from spores of Plasmopara halstedii, an obligate biotroph oomycete pathogen responsible for downy mildew in sunflower. We optimized the yield, the quality and the integrity of the extracted gDNA by fine-tuning three critical parameters, the grinding, the lysis temperature and the lysis duration. We obtained gDNA with a fragment size distribution reaching a peak ranging from 79 to 145 kb. More than half of the extracted gDNA consisted of DNA fragments larger than 42 kb, with 23% of fragments larger than 100 kb. We then demonstrated the relevance of this protocol for long-read se-quencing using PacBio RSII technology. With this protocol, we were able to obtain a mean read length of 9.3 kb, a max read length of 71 kb and an N50 of 13.3 kb. The development of such DNA extraction protocols is an essential prerequisite for fully exploiting technologies requiring high molecular weight gDNA (e.g. long-read sequencing or optical mapping). These technological advances will help generate data to answer questions such as the role of newly duplicated gene clusters, repeated regions, genomic structural variations or to define number of chromosomes that still remains undefined in many species of pathogenic fungi and oomycetes.
Published: 2020
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18. <scp>FAK</scp> activity in cancer‐associated fibroblasts is a prognostic marker and a druggable key metastatic player in pancreatic cancer

Author: Rémi Samain, Vinothini Rajeeve, Stéphane Pyronnet, Ismahane Belhabib, Manon Strehaiano, Stéphanie Cassant-Sourdy, Muriel Mathonnet, Yvan Martineau, Aurélie Perraud, Emilie Decaup, Cindy Neuzillet, Amandine Alard, Richard Tomasini, Corinne Bousquet, David D. Schlaepfer, Alexia Brunel, Christine Jean, Oliver M. T. Pearce, Jérôme Cros, Sonia Zaghdoudi, Julia Rochotte, Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées, University of California [San Diego] (UC San Diego), University of California, Hôpital Beaujon, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Institut Curie [Paris], Queen Mary University of London (QMUL), Université de Limoges (UNILIM), Barts Cancer Institute [London, UK], Cancer Research UK, CRUK: A27947 Fondation pour la Recherche Médicale, FRM: FRM ING20150532688, 40493 Ligue Contre le Cancer: RAB17029BBA, RMA04002BPA, RAB20008BBA PLBIO2015‐115, PAIR 2018‐080, IP/SC16060 G16001BB, We thank Dr Frédéric Lagarrigue (IPBS, Toulouse) for his help with beta‐1 integrin assay, and Marcin Domagala and Sophie Gazzola (CRCT, Toulouse) for their advice on macrophage experiments. We thank Laetitia Ligat (CRCT), Sophie Allart (CPTP) and Astrid Canivet‐Laffitte (CPTP) for their help and expertise in microscopy, Manon Farce (CRCT) for her help and flow cytometry expertise, and Loic Van Den Berghe and Christelle Segura for their help in lentivirus production and expertise in vectorology. The authors also acknowledge Dr Francois‐Xavier Frenois from the Imag'IN platform (IUC, Toulouse, France) for his help and expertise on Definiens Tissue Studio® and Dr Pedro Cutillas from the Centre for Haemato‐Oncology (Barts Cancer Institute, Queen Mary University of London, UK). We would like to thank Servier Medical Art ( https://smart.servier.com ) for graphics used in the figures. This work was supported by LNCC (Ligue Nationale Contre le Cancer RAB20007BBA, RAB20008BBA and RAB17029BBA), the Canceropole Grand Sud‐Ouest (RMA04002BPA), the Labex TOUCAN, the Toulouse University IDEX (G16001BB), the PHUC CAPTOR, la Fondation Bristol‐Myers Squibb and the French National Institute of Cancer (INCa, PLBIO2015‐115, PAIR 2018‐080). I.B is a recipient of LNCC fellowship (IP/SC16060). E.D. and J.R. salaries are funded, respectively, by Fondation pour la Recherche Médicale (FRM ING20150532688) and the French National Institute of Cancer (PLBIO2015‐115), and R.S. was a recipient of a fellowship from FRM (#40493). C.J. was recipient of a fellowship from the LNCC. O.M.T.P is a recipient of a Cancer Research UK and Credit Suisse fellowship (A27947).
Subjects: 0301 basic medicine, Medicine (General), Stromal cell, [SDV]Life Sciences [q-bio], pancreatic ductal adenocarcinoma, QH426-470, Biology, Article, extracellular matrix remodelling, Fibroblast migration, Metastasis, Focal adhesion, Extracellular matrix, 03 medical and health sciences, chemistry.chemical_compound, R5-920, 0302 clinical medicine, Cell Line, Tumor, Pancreatic cancer, Genetics, medicine, Humans, metastasis, Phosphorylation, Cancer, Biomarkers & Diagnostic Imaging, cancer‐associated fibroblasts, focal adhesion kinase, Tyrosine phosphorylation, Articles, Fibroblasts, Prognosis, medicine.disease, 3. Good health, Pancreatic Neoplasms, 030104 developmental biology, chemistry, Cancer research, Molecular Medicine, Cancer-Associated Fibroblasts, cancer-associated fibroblasts, 030217 neurology & neurosurgery, Carcinoma, Pancreatic Ductal
Abstract: Cancer‐associated fibroblasts (CAFs) are considered the most abundant type of stromal cells in pancreatic ductal adenocarcinoma (PDAC), playing a critical role in tumour progression and chemoresistance; however, a druggable target on CAFs has not yet been identified. Here we report that focal adhesion kinase (FAK) activity (evaluated based on 397 tyrosine phosphorylation level) in CAFs is highly increased compared to its activity in fibroblasts from healthy pancreas. Fibroblastic FAK activity is an independent prognostic marker for disease‐free and overall survival of PDAC patients (cohort of 120 PDAC samples). Genetic inactivation of FAK within fibroblasts (FAK kinase‐dead, KD) reduces fibrosis and immunosuppressive cell number within primary tumours and dramatically decreases tumour spread. FAK pharmacologic or genetic inactivation reduces fibroblast migration/invasion, decreases extracellular matrix (ECM) expression and deposition by CAFs, modifies ECM track generation and negatively impacts M2 macrophage polarization and migration. Thus, FAK activity within CAFs appears as an independent PDAC prognostic marker and a druggable driver of tumour cell invasion., Understanding how cancer‐associated fibroblasts (CAFs) promote PDAC progression is of major interest given the poor prognosis of patients. This study identifies a druggable key regulator of CAF‐induced tumour cell metastasis and a prognostic factor: the protein Focal Adhesion Kinase (FAK).
Published: 2020
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19. Intact cell MALDI-TOF mass spectrometry, a promising proteomic profiling method in farm animal clinical and reproduction research

Author: Elisabeth Blesbois, Laura Soler, Svetlana Uzbekova, Xavier Druart, Valérie Labas, Biosynthèse & Toxicité des Mycotoxines (ToxAlim-BioToMyc), ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur], This work was supported by the French National Infrastructure of Research CRB anim funded by 'Investissements d'avenir', ANR-11-INBS-0003 and from the French National Institute of Agronomic Research. The high-resolution mass spectrometer was financed (SMHART project) by the European Regional Development Fund (ERDF), the Conseil Regional du Centre, the French National Institute for Agricultural Research (INRA) and the French National Institute of Health and Medical Research (Inserm). Laura Soler has received the support of the EU in the framework of the Marie-Curie FP7 COFUND People Programme, through the award of an AgreenSkills fellowship (under grant agreement no 267196)., ANR-11-INBS-0003,CRB-Anim,Réseau de Centres de Ressources Biologiques pour les animaux domestiques(2011), European Project: 267196,EC:FP7:PEOPLE,FP7-PEOPLE-2010-COFUND,AGREENSKILLS(2012), Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Ecole d'Ingénieurs de Purpan (INP - PURPAN), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Subjects: Proteomics, MALDI-TOF, Oocyte, Livestock, media_common.quotation_subject, [SDV]Life Sciences [q-bio], Vitelline membrane, Computational biology, Biology, 03 medical and health sciences, 0302 clinical medicine, Food Animals, medicine, Animals, Small Animals, reproductive and urinary physiology, media_common, 030219 obstetrics & reproductive medicine, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, Mass spectrometry, Equine, Proteomic Profiling, Reproduction, 0402 animal and dairy science, Intact cell, 04 agricultural and veterinary sciences, MALDI-TOF Mass Spectrometry, 040201 dairy & animal science, Sperm, medicine.anatomical_structure, Gene Expression Regulation, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, ICM-MS, [SDV.SA.SPA]Life Sciences [q-bio]/Agricultural sciences/Animal production studies, Proteome, embryonic structures, Animal Science and Zoology, Single-Cell Analysis
Abstract: The objective of this review is to provide new insights into the possible use of a proteomic method known as Intact Cell Matrix-Assisted Laser Desorption-ionization Time-Of-Flight Mass Spectrometry (ICM-MS) in animal clinical research. Here, we give an overview of the basics of this technique, its advantages and disadvantages compared with other proteomic approaches, past applications and future perspectives. A special emphasis on its implementation in animal reproduction science is given, including examples of the reliable use of ICM-MS on fertility screening. In mammals, the ICM-MS profiles from pig epididymal spermatozoa reflect the proteome changes that they undergo during epididymal maturation and could be associated with the acquisition of fertilizing ability. In chicken, using adequate pre-processing and bioinformatics analysis tools, sperm ICM-MS profiles showed characteristic spectral features that allowed their classification according to their actual fertilizing ability. The association of ICM-MS and Top-down proteomic strategies allowed the identification of chicken fertility biomarkers candidates such as protein vitelline membrane outer layer protein 1 (VMO-1) and avian beta-defensin 10 (AvBD10). In female reproduction, a similar approach on ovarian follicular cells allowed the identification of specific markers of oocyte maturation in the oocyte and surrounding cumulus cells. Altogether, these results indicate that ICM-MS profiling could be a suitable approach for molecular phenotyping of male and female gametes.
Published: 2020
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20. Breastfeeding initiation or duration and longitudinal patterns of infections up to 2 years and skin rash and respiratory symptoms up to 8 years in the EDEN mother–child cohort

Author: Karine Adel-Patient, Blandine de Lauzon-Guillain, Sandrine Lioret, Barbara Heude, Camille Davisse-Paturet, Isabella Annesi-Maesano, Anne Forhan, Marie-Aline Charles, Equipe 3: EREN- Equipe de Recherche en Epidémiologie Nutritionnelle (CRESS - U1153), Université Sorbonne Paris Nord-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Service de Pharmacologie et Immunoanalyse (SPI), Médicaments et Technologies pour la Santé (MTS), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Etude longitudinale française depuis l'enfance (UMS : Ined-Inserm-EFS) (ELFE), EFS-Institut national d'études démographiques (INED)-Institut National de la Santé et de la Recherche Médicale (INSERM), National Institute for Research in Public health (IRESP: TGIR 2008 cohort in health programme), National Agency for Research (ANR nonthematic program), French speaking association for the study of diabetes and metabolism (ALFEDIAM), Mutuelle Générale de l'Education Nationale, Nestlé, French National Institute for Health Education (INPES), Paris–Sud University, French National Institute for Population Health Surveillance (InVS), French Ministry of Health, French Agency for Environment Security (AFSSET), Diabetes National Research Program (by a collaboration with the French Association of Diabetic Patients), INSERM Human Nutrition National Research Program, French Ministry of Research: Federative Research Institutes and Cohort Program, Fondation pour la Recherche Médicale, Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université (HESAM)-HESAM Université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université (HESAM)-HESAM Université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Institut national d'études démographiques (INED)-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), and HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Subjects: 0301 basic medicine, Male, Pediatrics, Time Factors, breastfeeding, Respiratory Tract Diseases, Breastfeeding, Cohort Studies, 0302 clinical medicine, Recurrence, longitudinal pattern, 030212 general & internal medicine, Prospective Studies, 2. Zero hunger, education.field_of_study, Nutrition and Dietetics, Incidence, Obstetrics and Gynecology, birth cohort, Rash, 3. Good health, Diarrhea, Breast Feeding, Bronchitis, Original Article, Female, France, medicine.symptom, Adult, medicine.medical_specialty, Population, Infections, 03 medical and health sciences, medicine, Humans, education, Asthma, 030109 nutrition & dietetics, business.industry, wheezing, Public Health, Environmental and Occupational Health, Infant, Original Articles, Exanthema, medicine.disease, infection, Otitis, Bronchiolitis, skin rash, Pediatrics, Perinatology and Child Health, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, business, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract: This paper aimed to examine the effect of breastfeeding on longitudinal patterns of common infections up to 2 years and respiratory symptoms up to 8 years. To assess the incidence and reoccurrence of infections and allergic symptoms in the first years of life among 1,603 children from the EDEN mother–child cohort, distinct longitudinal patterns of infectious diseases as well as skin rash and respiratory symptoms were identified by group‐based trajectory modelling. To characterize infections, we considered the parent‐reported number of cold/nasopharyngitis and diarrhoea from birth to 12 months and otitis and bronchitis/bronchiolitis from birth to 2 years. To characterize allergy‐related symptoms, we considered the parent‐reported occurrence of wheezing and skin rash from 8 months to 8 years and asthma from 2 to 8 years. Then associations between breastfeeding and these longitudinal patterns were assessed through adjusted multinomial logistic regression. Compared with never‐breastfed infants, ever‐breastfed infants were at a lower risk of diarrhoea events in early infancy as well as infrequent events of bronchitis/bronchiolitis throughout infancy. Only predominant breastfeeding duration was related to frequent events of bronchitis/bronchiolitis and infrequent events of otitis. We found no significant protective effect of breastfeeding on longitudinal patterns of cold/nasopharyngitis, skin rash, or respiratory symptoms. For an infant population with a short breastfeeding duration, on average, our study confirmed a protective effect of breastfeeding on diarrhoea events in early infancy, infrequent bronchitis/bronchiolitis and, to a lesser extent, infrequent otitis events up to 2 years but not on other infections, skin rash, or respiratory symptoms4., In an infant population with a short breastfeeding duration and using longitudinal patterns of infection, skin rash and respiratory symptoms, on average, our study confirmed a protective effect of breastfeeding on diarrhea events in early infancy, infrequent bronchitis/bronchiolitis and, to a lesser extent,infrequent otitis events up to 2 years but not on cold/ nasopharyngitis, skin rash or respiratory symptoms.
Published: 2020
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21. Impulsivity and consideration of future consequences as moderators of the association between emotional eating and body weight status

Author: Marc Bénard, France Bellisle, Fabrice Etilé, Gérard Reach, Emmanuelle Kesse-Guyot, Serge Hercberg, Sandrine Péneau, Institut de Chimie de Strasbourg, Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), Unité de Recherche en Epidémiologie Nutritionnelle (UREN), Université Paris 13 (UP13)-Institut National de la Recherche Agronomique (INRA)-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Paris Jourdan Sciences Economiques (PJSE), Université Panthéon-Sorbonne (UP1)-École normale supérieure - Paris (ENS Paris)-Institut National de la Recherche Agronomique (INRA)-École des hautes études en sciences sociales (EHESS)-École des Ponts ParisTech (ENPC)-Centre National de la Recherche Scientifique (CNRS), Paris School of Economics (PSE), Hotel-Dieu, Service de Diabétologie, Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Bénard, Marc, Université Louis Pasteur - Strasbourg I-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université Paris 13 (UP13)-Institut National de la Recherche Agronomique (INRA)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Equipe 3: EREN- Equipe de Recherche en Epidémiologie Nutritionnelle (CRESS - U1153), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris 1 Panthéon-Sorbonne (UP1)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École des hautes études en sciences sociales (EHESS)-École des Ponts ParisTech (ENPC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Hôpital Avicenne [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), French Ministry of Health (DGS), Sante Publique France agency, French National Institute for Health and Medical Research (INSERM), French National Institute for Agricultural Research (INRA), National Conservatory for Arts and Crafts (CNAM), Medical Research Foundation (FRM), University of Paris 13, French National Institute for Agricultural Research (Institut National de la Recherche Agronomique), Centre National de la Recherche Scientifique (CNRS)-Université Louis Pasteur - Strasbourg I-Institut de Chimie du CNRS (INC), Université Paris 13 (UP13)-Institut National de la Recherche Agronomique (INRA)-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA), Université Paris 1 Panthéon-Sorbonne (UP1)-École normale supérieure - Paris (ENS Paris), École des Ponts ParisTech (ENPC)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris 1 Panthéon-Sorbonne (UP1)-Centre National de la Recherche Scientifique (CNRS)-École des hautes études en sciences sociales (EHESS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Sorbonne Paris Cité (USPC)-Université Paris 13 (UP13)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université (HESAM)-HESAM Université (HESAM)-Institut National de la Recherche Agronomique (INRA), and HESAM Université (HESAM)-HESAM Université (HESAM)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153))
Subjects: Male, 0301 basic medicine, Emotional eating, Emotions, Medicine (miscellaneous), Weight Gain, Body Mass Index, Nutritional status, Consideration of future consequences, Impulsivity, Psychology, Cohort Studies, Eating, Surveys and Questionnaires, lcsh:RC620-627, ComputingMilieux_MISCELLANEOUS, Nutrition and Dietetics, lcsh:Public aspects of medicine, Regression analysis, Middle Aged, lcsh:Nutritional diseases. Deficiency diseases, Female, medicine.symptom, Cohort study, Adult, Physical Therapy, Sports Therapy and Rehabilitation, Clinical nutrition, 03 medical and health sciences, Barratt Impulsiveness Scale, medicine, Humans, Obesity, Life Style, Aged, 030109 nutrition & dietetics, business.industry, Research, Body Weight, Repeated measures design, lcsh:RA1-1270, Feeding Behavior, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, Impulsive Behavior, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Self Report, business, Demography
Abstract: Background Emotional eating (EmE) is characterized by an over consumption of food in response to negative emotions and is associated with an increased weight status. Consideration of Future Consequences (CFC) or a low level of impulsivity could influence the association between EmE and weight status. The objective was to analyze the moderating influence of CFC and impulsivity on the relationship between EmE and BMI. Methods A total of 9974 men and 39,797 women from the NutriNet-Santé cohort study completed the revised 21-item Three-Factor Eating Questionnaire to assess their EmE, the CFC questionnaire (CFC-12) to assess their level of time perspective, and the Barratt Impulsiveness Scale (BIS-11) to assess their impulsivity. Weight and height were self-reported each year over a median follow-up of 5.3 years. The associations between EmE and repeated measures of BMI were estimated by multiple linear mixed-effects regression models stratified by gender, tertiles of the CFC, or tertiles of the BIS-11, taking into account sociodemographic and lifestyle factors. Results Overall, EmE was positively associated with BMI. CFC and impulsivity did not moderate the effect of EmE on changes of BMI per year, but quantitatively moderated the effect of EmE on overall BMI. In women, the strength of the association between EmE and weight status increased with CFC level. Difference of BMI slopes between a low and a high level of CFC was − 0.43 kg/m2 (95% CI: -0.55, − 0.30) (p
Published: 2018
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22. Binder of Sperm Proteins protect ram spermatozoa from freeze-thaw damage

Author: Xavier Druart, Valérie Labas, Simon P. de Graaf, Kiri Farmer, Ana Paula Teixeira-Gomes, Guillaume Tsikis, Taylor Pini, T. Leahy, University of Sydney, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA), Australian Wool Innovation [ON_00252], NSW Stud Merino Breeders Association, European Regional Development Fund [3069], Conseil Regional du Centre, French National Institute for Agricultural Research, French National Institute of Health and Medical Research (INSERM), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
Subjects: Male, endocrine system, Cryoprotectant, Seminal Plasma Proteins, [SDV]Life Sciences [q-bio], Motility, General Biochemistry, Genetics and Molecular Biology, Cryopreservation, Andrology, 03 medical and health sciences, chemistry.chemical_compound, Cryoprotective Agents, 0302 clinical medicine, Semen, Binder of sperm proteins, Freezing, Animals, [INFO]Computer Science [cs], Phosphatidylethanolamine, Sheep, 030219 obstetrics & reproductive medicine, urogenital system, Phosphatidylethanolamines, 0402 animal and dairy science, Tyrosine phosphorylation, 04 agricultural and veterinary sciences, General Medicine, Spermatozoa, 040201 dairy & animal science, Mucus, Sperm, Ovine, Fertility, chemistry, Sperm Motility, Cattle, General Agricultural and Biological Sciences, Semen Preservation
Abstract: International audience; Cryopreservation causes sub-lethal damage which limits the fertility of frozen thawed spermatozoa. Seminal plasma has been investigated as a cryoprotectant, but has yielded inconsistent results due to considerable variation in its constituents. Individual seminal plasma proteins offer an ideal alternative to whole seminal plasma, and several have been correlated with freezing success. Binder of Sperm Proteins (BSPs) are abundant ram seminal plasma proteins which have been suggested to have significant protective effects on ram spermatozoa during cold shock. This is in direct opposition to bull spermatozoa, where BSPs cause sperm deterioration during in vitro handling. We investigated the potential of BSP1 and BSP5 to prevent freezing associated damage to important functional parameters of ram spermatozoa. BSPs purified by size exclusion chromatography improved post thaw motility and penetration through artificial mucus. Highly purified BSP1 and BSP5, isolated by gelatin affinity and RP-HPLC, improved motility and membrane integrity, and reduced post thaw protein tyrosine phosphorylation. Exposure to BSP5 before freezing increased the amount of phosphatidylethanolamine on the sperm surface after thawing. Neither BSP1 nor BSP5 prevented freezing associated changes in membrane lipid disorder. These results suggest that BSPs may significantly improve freezing outcomes of ram spermatozoa.
Published: 2018
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23. Human health risks related to the consumption of foodstuffs of animal origin contaminated by bisphenol A

Author: Gorecki, Sébastien, Bemrah, Nawel, Roudot, Alain-Claude, Marchioni, Eric, Le Bizec, Bruno, Faivre, Franck, Kadawathagedara, Manik, Botton, Jérémie, Rivière, Gilles, EDEN mother-child cohort study group, ., Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Université de Bretagne Occidentale, Université de Strasbourg (UNISTRA), Laboratoire d'étude des Résidus et Contaminants dans les Aliments (LABERCA), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), LUNAM Université [Nantes Angers Le Mans], Ministère de l'Agriculture et de l'Alimentation, Service de biostatistique et information médicale de l’hôpital Saint Louis (Equipe ECSTRA) (SBIM), Hopital Saint-Louis [AP-HP] (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut national du cancer [Boulogne] (INCA)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Faculty of pharmacy, Université Paris-Sud - Paris 11 (UP11), Institut National de la Santé et de la Recherche Médicale (INSERM), Foundation for medical research (FRM), National Agency for Research (ANR) [ANR-06-BLAN-0359], National Institute for Research in Public health (IRESP: TGIR cohorte santé 2008 program), French Ministry of Health (DGS), French Ministry of Research, INSERM Bone and Joint Diseases National Research (PRO-A) and Human Nutrition National Research Programs, Paris-Sud University, Nestlé, French National Institute for Population Health Surveillance (InVS), French National Institute for Health Education (INPES), European Union FP7 programme (HELIX project), European Union FP7 programme (ESCAPE project), European Union FP7 programme (ENRIECO project), Diabetes National Research Program through a collaboration with the French Association of Diabetic Patients (AFD), French Agency for Environmental Health Safety, Mutuelle Générale de l'Education Nationale a complementary health insurance (MGEN), French national agency for food security, French speaking association for the study of diabetes and metabolism (ALFEDIAM), European Project: 261357,EC:FP7:HEALTH,FP7-HEALTH-2010-single-stage,MEDALL(2010), Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut national du cancer [Boulogne] (INCA)-Hopital Saint-Louis [AP-HP] (AP-HP), and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)
Subjects: Male, Animal food, Food contamination, 010501 environmental sciences, Toxicology, 01 natural sciences, Poultry, Non-canned food, Bisphenol A, Pregnancy, Food science, Child, Foodstuffs of animal origin, 2. Zero hunger, education.field_of_study, Fishes, 04 agricultural and veterinary sciences, General Medicine, Middle Aged, Contamination, 040401 food science, Food Analysis, 3. Good health, Conjugates, Child, Preschool, Female, France, Adult, Meat, Adolescent, Population, Biology, Young Adult, Health risk assessment, 0404 agricultural biotechnology, Phenols, Environmental health, Animals, Humans, Benzhydryl Compounds, education, Aged, 0105 earth and related environmental sciences, Exposure assessment, business.industry, Consumer Product Safety, Food processing, business, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Food Science, Food contaminant
Abstract: International audience; Bisphenol A (BPA) is used in a wide variety of products and objects for consumers use (digital media such as CD's and DVD's, sport equipment, food and beverage containers, medical equipment). For humans, the main route of exposure to BPA is food. Based on previous estimates, almost 20% of the dietary exposure to BPA in the French population would be from food of animal origin. However, due to the use of composite samples, the source of the contamination had not been identified. Therefore, 322 individual samples of non-canned foods of animal origin were collected with the objectives of first updating the estimation of the exposure of the French population and second identifying the source of contamination of these foodstuffs using a specific analytical method. Compared to previous estimates in France, a decline in the contamination of the samples was observed, in particular with regard to meat. The estimated mean dietary exposures ranged from 0.048 to 0.050 μg (kg bw)(-1) d(-1) for 3-17 year children and adolescents, from 0.034 to 0.035 μg (kg bw)(-1) d(-1) for adults and from 0.047 to 0.049 μg (kg bw)(-1) d(-1) for pregnant women. The contribution of meat to total dietary exposure of pregnant women, adults and children was up to three times lower than the previous estimates. Despite this downward trend in contamination, the toxicological values were observed to have been exceeded for the population of pregnant women. With the aim of acquiring more knowledge about the origin the potential source(s) of contamination of non-canned foods of animal origin, a specific analytical method was developed to directly identify and quantify the presence of conjugated BPA (BPA-monoglucuronide, BPA-diglucuronide and sulphate forms) in 50 samples. No conjugated forms of BPAs were detected in the analysed samples, indicating clearly that BPA content in animal food was not due to metabolism but arise post mortem in food. This contamination may occur during food production. However, despite extensive sampling performed in several different shops (butcheries, supermarkets ....) and in different conditions (fresh, prepared, frozen ...), the source(s) of the contamination could not be specifically identified.
Published: 2017
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24. Adherence to the 2017 French dietary guidelines and adult weight gain: A cohort study

Author: Mathilde Touvier, Chantal Julia, Dan Chaltiel, Serge Hercberg, Emmanuelle Kesse-Guyot, Moufidath Adjibade, Equipe 3: EREN- Equipe de Recherche en Epidémiologie Nutritionnelle (CRESS - U1153), Institut National de la Recherche Agronomique (INRA)-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Université Paris 13 (UP13)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Département de Santé Publique [Avicenne], Hôpital Avicenne [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), The NutriNet-Santé Study is supported by the French Ministry of Health (https://solidaritessante. gouv.fr/ministere/organisation/directions/ article/dgs-direction-generale-de-la-sante), the French Public Health Agency (http://www.santepubliquefrance.fr), the French National Institute for Health and Medical Research (INSERM, www.inserm.fr), the French National Institute for Agricultural Research (INRA, www.inra.fr), the National Conservatory for Arts and Crafts (CNAM, www.cnam.fr) and the Paris 13 University (www.univ-paris13.fr)., Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Sorbonne Paris Nord-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département de Santé Publique [Bobigny] (Hôpital Avicenne), Hôpital Avicenne, Kesse-guyot, Emmanuelle, Université Paris 13 (UP13)-Institut National de la Recherche Agronomique (INRA)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), and HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153))
Subjects: Male, Physiology, [SDV]Life Sciences [q-bio], Health Behavior, 030204 cardiovascular system & hematology, Overweight, Weight Gain, Body Mass Index, Nutrition Policy, Cohort Studies, 0302 clinical medicine, Animal Products, Medicine and Health Sciences, Public and Occupational Health, Prospective Studies, 030212 general & internal medicine, 10. No inequality, Prospective cohort study, 2. Zero hunger, Alcohol Consumption, Eukaryota, Agriculture, General Medicine, Plants, Middle Aged, Legumes, Nutrition Surveys, 3. Good health, [SDV] Life Sciences [q-bio], Physiological Parameters, Cohort, Medicine, Female, France, medicine.symptom, Research Article, Cohort study, Adult, Meat, Adolescent, Lower risk, Young Adult, 03 medical and health sciences, medicine, Humans, Obesity, Nutrition, business.industry, Proportional hazards model, Body Weight, Food Consumption, Organisms, Biology and Life Sciences, Physical Activity, Feeding Behavior, medicine.disease, Diet, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, Food, Physiological Processes, business, Body mass index, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Demography
Abstract: Background The French dietary guidelines were updated in 2017, and an adherence score to the new guidelines (Programme National Nutrition Santé Guidelines Score 2 [PNNS-GS2]) has been developed and validated recently. Since overweight and obesity are key public health issues and have been related to major chronic conditions, this prospective study aimed to measure the association between PNNS-GS2 and risk of overweight and obesity, and to compare these results with those for the modified Programme National Nutrition Santé Guidelines Score (mPNNS-GS1), reflecting adherence to 2001 guidelines. Methods and findings Participants (N = 54,089) were recruited among French adults (≥18 years old, mean baseline age = 47.1 [SD 14.1] years, 78.3% women) in the NutriNet-Santé web-based cohort. Mean (SD) score was 1.7 (3.3) for PNNS-GS2 and 8.2 (1.6) for mPNNS-GS1. Selected participants were those included between 2009 and 2014 and followed up to September 2018 (median follow-up = 6 years). Collected data included at least three 24-hour dietary records over a 2-year period following inclusion, baseline sociodemographics, and anthropometric data over time. In Cox regression models, PNNS-GS2 was strongly and linearly associated with a lower risk of overweight and obesity (HR for quintile 5 versus quintile 1 [95% CI] = 0.48 [0.43–0.54], p < 0.001, and 0.47 [0.40–0.55], p < 0.001, for overweight and obesity, respectively). These results were much weaker for mPNNS-GS1 (HR for quintile 5 versus quintile 1 = 0.90 [0.80–0.99], p = 0.03, and 0.98 [0.84–1.15], p = 0.8, for overweight and obesity, respectively). In multilevel models, PNNS-GS2 was negatively associated with baseline BMI and BMI increase over time (β for a 1-SD increase in score [95% CI] = −0.040 [−0.041; −0.038], p < 0.001, and −0.00080 [−0.00094; −0.00066], p < 0.001, respectively). In “direct comparison” models, PNNS-GS2 was associated with a lower risk of overweight and obesity, lower baseline BMI, and lower BMI increase over time than mPNNS-GS1. Study limitations include possible selection bias, reliance on participant self-report, use of arbitrary cutoffs in data analyses, and residual confounding, but robustness was tested in sensitivity analyses. Conclusions Our findings suggest that adherence to the 2017 French dietary guidelines is associated with a lower risk of overweight and obesity. The magnitude of the association and the results of the direct comparison reinforced the validity of the updated recommendations. Trial registration The NutriNet-Santé Study ClinicalTrials.gov (NCT03335644), Dan Chaltiel and colleagues assess the association between adherence to 2017 French dietary recommendation and the risk of weight gain., Author summary Why was this study done? Obesity is a major condition, growing worldwide at a pandemic rate, and represents an important risk factor for main non-communicable diseases like cardiovascular diseases, type II diabetes, and some types of cancer. In public health, weight management is a critical lever to limit the occurrence of obesity in the population, and nutrition is an important part of it. French food-based dietary guidelines were updated in 2017, and a dietary score, PNNS-GS2, was developed to measure their level of adherence in the population. As part of evaluation of these guidelines, it was necessary to study the association between PNNS-GS2 and BMI change and the risk of developing overweight or obesity. What did the researchers do and find? We used statistical models to capture the effect of following the new dietary guidelines (reflected by a high PNNS-GS2) on weight. We used data from 54,089 participants of the French NutriNet-Santé cohort, with a median follow-up of 6 years. Having a high PNNS-GS2 was associated with lower weight gain and a lower risk of developing overweight or obesity. Another model allowed comparison of PNNS-GS2 to mPNNS-GS1, reflecting the former French dietary guidelines (2001), and PNNS-GS2 performed significantly better than its predecessor regarding the outcomes. What do these findings mean? Following the 2017 French dietary guidelines can be expected to improve weight management in the French population. It is highly probable that this will also reduce the incidence of chronic diseases, as BMI is a strong risk factor. The next step will be to test the association of PNNS-GS2 with chronic diseases.
Published: 2019
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25. Identification of potential chemosignals in the European water vole Arvicola terrestris

Author: Nagnan-Le Meillour, Patricia, Descamps, Amandine, Le Danvic, Chrystelle, Grandmougin, Maurane, Saliou, Jean-Michel, Klopp, Christophe, MILHES, Marine, Bompard, Coralie, Chesneau, Didier, Poissenot, Kévin, Keller, Matthieu, Université de Lille, CNRS, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF], Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL], Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Unité de Biométrie et Intelligence Artificielle (ancêtre de MIAT) (UBIA), Institut National de la Recherche Agronomique (INRA), Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), région Auvergne-Rhône-Alpes Conseil Regional Auvergne-Rhone-Alpes 1250 French National Institute for Agronomic Research (INRA), Université de Lille-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Unité de Biométrie et Intelligence Artificielle (UBIA), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Neurobiologie de l'Olfaction et de la Prise Alimentaire (NOPA), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Sciences Analytiques et Interactions Ioniques et Biomoléculaires (DSA-IPHC), Institut Pluridisciplinaire Hubert Curien (IPHC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), région Auvergne-Rhône-Alpes Conseil Regional Auvergne-Rhone-Alpes 1250 French National Institute for Agronomic Research (INRA) French National Research Agency (ANR) ANR-10-INBS-09, Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Allice, Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Coeur Poumon [CHU Lille], Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] (IRI), and Université de Lille, Sciences et Technologies-Université de Lille, Droit et Santé-Centre National de la Recherche Scientifique (CNRS)
Subjects: Male, Proteomics, HOUSE MOUSE, [SDV]Life Sciences [q-bio], Population Dynamics, PROTEIN, lcsh:Medicine, water vole, ARVICOLIN, Chemical ecology, Article, PHEROMONES, Animals, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Scent Glands, Sex Attractants, lcsh:Science, ComputingMilieux_MISCELLANEOUS, POPULATION, ODORANT-BINDING PROTEIN, APHRODISIN, LIPOCALIN, URINARY VOLATILE COMPOUNDS, Arvicolinae, [SDV.BA]Life Sciences [q-bio]/Animal biology, lcsh:R, Small molecules, Fatty Acids, Volatile, Lipocalins, United Kingdom, O-GlcNAc, odorant-binding protein, urinary volatile compounds, pheromones, lipocalin, population, arvicolin, Liver, AGGRESSION, HAMSTER, RNA, lcsh:Q, Female, France, Seasons, Post-translational modifications
Abstract: International audience; The water vole Arvicola terrestris is endemic to Europe where its outbreak generates severe economic losses for farmers. Our project aimed at characterising putative chemical signals used by this species, to develop new sustainable methods for population control that could also be used for this species protection in Great Britain. The water vole, as well as other rodents, uses specific urination sites as territorial and sex pheromone markers, still unidentified. Lateral scent glands and urine samples were collected from wild males and females caught in the field, at different periods of the year. Their volatile composition was analysed for each individual and not on pooled samples, revealing a specific profile of flank glands in October and a specific profile of urinary volatiles in July. The urinary protein content appeared more contrasted as males secrete higher levels of a lipocalin than females, whenever the trapping period. We named this protein arvicolin. Male and female liver transcript sequencing did not identify any expression of other odorant-binding protein sequence. This work demonstrates that even in absence of genome, identification of chemical signals from wild animals is possible and could be helpful in strategies of species control and protection.
Published: 2019
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26. Optimism is associated with diet quality, food group consumption and snacking behavior in a general population

Author: Wassila Ait-hadad, Marc Bénard, Serge Hercberg, Mathilde Touvier, Sandrine Péneau, Rebecca Shankland, Emmanuelle Kesse-Guyot, Camille Buscail, Margaux Robert, Equipe 3: EREN- Equipe de Recherche en Epidémiologie Nutritionnelle (CRESS - U1153), Université Paris 13 (UP13)-Institut National de la Recherche Agronomique (INRA)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Inter-universitaire de Psychologie : Personnalité, Cognition, Changement Social (LIP-PC2S), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Grenoble Alpes (UGA), Hôpital Avicenne [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Federation of European Nutrition Societies (FENS), AUDOUX, Michèle, Université Sorbonne Paris Nord-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), French Ministry of Health (DGS) Sante Publique France agencyInstitut National de la Sante et de la Recherche Medicale (Inserm)French National Institute for Agricultural Research (INRA)National Conservatory for Arts and Crafts (CNAM)Fondation pour la Recherche MedicaleUniversity of Paris 13 French National Institute for Agricultural Research (Institut National de la Recherche Agronomique), Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Grenoble Alpes (UGA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), and Institut National de la Recherche Agronomique (INRA)-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Université Paris 13 (UP13)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153))
Subjects: Male, 030309 nutrition & dietetics, media_common.quotation_subject, [SDV]Life Sciences [q-bio], Population, Medicine (miscellaneous), lcsh:TX341-641, 030209 endocrinology & metabolism, Diet Surveys, Food group, 03 medical and health sciences, 0302 clinical medicine, Optimism, Environmental health, Food Quality, Humans, Psychology, Eating behavior, education, lcsh:RC620-627, Cross-sectional study, ComputingMilieux_MISCELLANEOUS, media_common, 2. Zero hunger, Consumption (economics), 0303 health sciences, education.field_of_study, Nutrition and Dietetics, Snacking, Research, Feeding Behavior, Middle Aged, Diet, [SDV] Life Sciences [q-bio], lcsh:Nutritional diseases. Deficiency diseases, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, Cross-Sectional Studies, Diet quality, Female, France, Snacks, lcsh:Nutrition. Foods and food supply, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract: Background Dispositional optimism is a psychological trait that has been associated with positive health outcomes such as reduced risk of cardiovascular diseases. However, there is little knowledge on the relationship between optimism and dietary intake in the population. The objective of this cross-sectional study was to assess whether optimism was associated with overall diet quality, food group consumption and snacking. Methods In 2016, 32,806 adult participants from the NutriNet-Santé study completed the Life-Orientation Test Revised (LOT-R) which assesses dispositional optimism. Overall diet quality (assessed by the mPNNS-Guideline Score) and consumption of 22 food groups were evaluated using at least three self-reported 24-h dietary records. Snacking behavior was evaluated by an ad-hoc question. Logistic and linear regressions were used to analyze the associations between optimism and these dietary behaviors, taking into account socio-demographic, lifestyle and depressive symptomatology characteristics. Results Optimism was associated with greater overall diet quality (β (95% CI) = 0.07 (0.004–0.11), P
Published: 2019
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27. Association between genetic obesity susceptibility and mother-reported eating behavior in children up to 5 years

Author: De Lauzon-Guillain, Blandine, Koudou, Yves, Botton, Jérémie, Forhan, Anne, Carles, Sophie, Pelloux, Véronique, Clément, Karine, Ong, Ken, Charles, Marie Aline, Heude, Barbara, Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Equipe 6 : ORCHAD - Origines précoces de la santé du développement de l'enfant (CRESS - U1153), Université Paris Descartes - Paris 5 (UPD5)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris-Sud - Paris 11 (UP11), Université Paris-Saclay, Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Institute of cardiometabolism and nutrition (ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [APHP]-Sorbonne Université (SU), Medical Research Council Epidemiology Unit [Cambridge, UK] (Department of Paediatrics), University of Cambridge [UK] (CAM)-Addenbrooke’s Hospital [Cambridge, UK], The EDEN study is supported by: Fondation pour la Recherche Médicale (FRM), French Ministry of Research: Federative Research Institutes and Cohort Program, INSERM Human Nutrition National Research Program, and Diabetes National Research Program (through a collaboration with the French Association of Diabetic Patients (AFD)), French Ministry of Health, French Agency for Environment Security (AFSSET), French National Institute for Population Health Surveillance (InVS), Paris–Sud University, French National Institute for Health Education (INPES), Nestlé, Mutuelle Générale de l’Education Nationale (MGEN), French speaking association for the study of diabetes and metabolism (ALFEDIAM), National Agency for Research (ANR non thematic program), National Institute for Research in Public health (IRESP: TGIR 2008 cohort in health program). The genotyping was funded by a Collaborative Research Grant from the European Society for Paediatric Endocrinology. KKO is supported by the Medical Research Council (Unit program: MC_UU_12015/2)., Members of the EDEN Mother-Child Cohort Study Group: I. Annesi-Maesano, JY. Bernard, J. Botton, M.A. Charles, P. Dargent-Molina, B. de Lauzon-Guillain, P. Ducimetière, M. de Agostini, B. Foliguet, A. Forhan, X. Fritel, A. Germa, V. Goua, R. Hankard, B. Heude, M. Kaminski, B. Larroque†, N. Lelong, J. Lepeule, G. Magnin, L. Marchand, C. Nabet, F. Pierre, R. Slama, M.J. Saurel-Cubizolles, M. Schweitzer, O. Thiebaugeorges., Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA), Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), and Addenbrooke’s Hospital [Cambridge, UK]-University of Cambridge [UK] (CAM)
Subjects: eating behaviour, growth, birth cohort, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, genetic
Abstract: International audience; BACKGROUND:Many genetic polymorphisms identified by genome-wide association studies for adult body mass index (BMI) have been suggested to regulate food intake.OBJECTIVE:The objective was to study the associations between a genetic obesity risk score, appetitive traits, and growth of children up to age 5 years, with a longitudinal design.METHODS:In 1142 children from the Etude des Déterminants pre et post natals de la santé de l'ENfant (EDEN) birth cohort, a combined obesity risk-allele score (BMI genetic risk score [GRS]) was related to appetitive traits (energy intake up to 12 mo, a single item on appetite from 4 mo to 3 y, a validated appetite score at 5 y) using Poisson regressions with robust standard errors. The potential mediation of appetitive traits on the association between BMI-GRS and growth was assessed by the Sobel test.RESULTS:Children with a high BMI-GRS were more likely to have high energy intake at 1 year and high appetite at 2 and 5 years. High energy intake in infancy and high appetite from 1 year were related to higher subsequent BMI. High 2-year appetite seemed to partially mediate the associations between BMI-GRS and BMI from 2 to 5 years (all P ≤ 0.05).CONCLUSIONS:Genetic susceptibility to childhood obesity seems to be partially explained by appetitive traits in infancy, followed by an early childhood rise in BMI.
Published: 2019
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28. Variation in the Venom of Parasitic Wasps, Drift, or Selection? Insights From a Multivariate QST Analysis

Author: Mathe-Hubert, Hugo, Kremmer, Laurent, Colinet, Dominique, Gatti, Jean-Luc, van Baaren, Joan, Delava, Emilie, Poirie, Marylène, Institut Sophia Agrobiotech (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), Génétique et évolution des interactions hôtes-parasites, Département génétique, interactions et évolution des génomes [LBBE] (GINSENG), Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), This work received support from the French National Research Agency (CLIMEVOL project, ANR-08-BLAN-0231) and the Department of Plant Health and Environment from the French National Institute for Agricultural Research (INRA). It was performed in the context of the Investments for the Future LABEX SIGNALIFE: program reference ANR-11-LABX-0028. HM-H was funded by the Provence Alpes Côte d’Azur (PACA) regionand the Department of Plant Health and Environment from the French National Institute for Agricultural Research (INRA)., ANR-08-BLAN-0231,CLIMEVOL,Climate change and Evolution of host-parasitoid interactions: from molecules to communities(2008), ANR-11-LABX-0028,SIGNALIFE,Réseau d'Innovation sur les Voies de Signalisation en Sciences de la Vie(2011), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Institut Sophia Agrobiotech [Sophia Antipolis] (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Centre National de la Recherche Scientifique (CNRS), and ANR: 11-LABX-0028,SIGNALIFE,Réseau d'Innovation sur les Voies de Signalisation en Sciences de la Vie(2011)
Subjects: adaptive divergence, Leptopilina wasps, [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE], antagonistic coevolution, lcsh:Evolution, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, multivariate QST, lcsh:QH540-549.5, lcsh:QH359-425, Drosophila, lcsh:Ecology, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, population proteomics, individual 1D SDS-PAGE, local adaptation
Abstract: International audience; Differentiation of traits among populations can evolve by drift when gene flow is low relative to drift or selection when there are different local optima in each population (heterogeneous selection), whereas homogeneous selection tends to prevent evolution of such a differentiation. Analyses of geographical variations in venom composition have been done in several taxa such as wasps, spiders, scorpions, cone snails and snakes, but surprisingly never in parasitoid wasps, although their venom should constrain their ability to succeed on locally available hosts. Such a study is now facilitated by the development of an accurate method (quantitative digital analysis) that allows analyzing the quantitative variation of large sets of proteins from several individuals. This method was used here to analyse the venom-based differentiation of four samples of Leptopilina boulardi and five samples of L. heterotoma from populations along a 300 km long south-north gradient in the Rhône-Saône valley (South-East of France). A major result is that the composition of the venom allows to differentiate the populations studied even when separated by few kilometers. We further analyzed these differentiations on the populations (reared under similar conditions to exclude environmental variance) with a QST analysis which compared the variance of a quantitative trait (Q) among the subpopulations (S) to the total variance (T). We also used random forest clustering analyses to detect the venom components the most likely to be adapted locally. The signature of the natural selection was strong for L. heterotoma and L. boulardi. For the latter, the comparison with the differentiation observed at some neutral markers revealed that differentiation was partly due to some local adaptation. The combination of methods used here appears to be a powerful framework for population proteomics and for the study of eco-evolutionary feedbacks between proteomic level and population and ecosystem levels. This is of interest not only for studying field evolution at an intermediate level between the genome and phenotypes, or for understanding the role of evolution in chemical ecology, but also for more applied issues in biological control.
Published: 2019
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29. Data on endogenous chicken sperm peptides and small proteins obtained through Top-Down High Resolution Mass Spectrometry

Author: Laura Bouguereau, Isabelle Grasseau, L. Soler, Elisabeth Blesbois, Valérie Labas, Aurore Thélie, Ana-Paula Teixeira-Gomes, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Plate-forme d'Analyse Intégrative des Biomolécules, Institut National de la Recherche Agronomique (INRA), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, French National Infrastructure of Research CRB anim funded by 'Investissements d׳avenir', ANR-11-INBS-0003, (SMHART project) by the European Regional Development Fund (ERDF), Conseil Régional du Centre, French National Institute for Agricultural Research (INRA), French National Institute of Health and Medical Research (Inserm), European Project: 267196,EC:FP7:PEOPLE,FP7-PEOPLE-2010-COFUND,AGREENSKILLS(2012), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], sperme, chicken, poulet, Context (language use), Endogeny, Peptide, Biology, lcsh:Computer applications to medicine. Medical informatics, Mass spectrometry, Proteomics, sperm, 03 medical and health sciences, spectrométrie de masse, top-down HRMS, peptidome, lcsh:Science (General), Data Article, chemistry.chemical_classification, modèle en top down, Multidisciplinary, Chromatography, Intact cell, Sperm, 030104 developmental biology, Biochemistry, chemistry, Male fertility, lcsh:R858-859.7, Autre (Sciences du Vivant), lcsh:Q1-390
Abstract: The endogenous peptides and small proteins present in chicken sperm were identified in the context of the characterization of a fertility-diagnostic method based on the use of ICM-MS (Intact Cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry). The interpretation and description of these data can be found in a research article, “Intact cell MALDI-TOF MS on sperm: a molecular test for male fertility diagnosis” (Soler et al., 2016) [1], and raw data derived from this analysis have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PRIDE: PXD002768. Here, we describe the inventory of all the molecular species identified, along with their biochemical features and functional analysis. This peptide/protein catalogue can be further employed as reference for other studies and reveal that the use of proteomics allows for a global evaluation of sperm cells functions. Keywords: Chicken, Sperm, Top-Down HRMS, Peptidome
Published: 2016
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30. Prenatal diet and children's trajectories of hyperactivity-inattention and conduct problems from 3 to 8 years: the EDEN mother-child cohort

Author: Cédric Galéra, Hugo Peyre, Jonathan Y. Bernard, Maria Melchior, Blandine de Lauzon-Guillain, Laura Pryor, A. Forhan, Manuel-Pierre Bouvard, Sandrine Lioret, Barbara Heude, Centre hospitalier Charles Perrens [Bordeaux], Bordeaux population health (BPH), Université de Bordeaux (UB)-Institut de Santé Publique, d'Épidémiologie et de Développement (ISPED)-Institut National de la Santé et de la Recherche Médicale (INSERM), Equipe 6 : ORCHAD - Origines précoces de la santé du développement de l'enfant (CRESS - U1153), Université Paris Descartes - Paris 5 (UPD5)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Agency for science, technology and research [Singapore] (A*STAR), AP-HP Hôpital universitaire Robert-Debré [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Laboratoire de sciences cognitives et psycholinguistique (LSCP), Département d'Etudes Cognitives - ENS Paris (DEC), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École des hautes études en sciences sociales (EHESS)-Centre National de la Recherche Scientifique (CNRS), Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Université Pierre et Marie Curie - Paris 6 (UPMC), Charles Perrens Hospital, Department of Child and Adolescent Psychiatry, Université de Bordeaux (UB), Fondation pour la Recherche Medicale, French Ministry of Research Institut Federatif de Recherche, Institut National de la Sante et de la Recherche Medicale (Inserm), French Ministry of Health Perinatal Program, French Agency for Environment Security, French National Institute for Population Health Surveillance, Paris-Sud University, French National Institute for Health Education, Nestle, Mutuelle Generale de l'Education Nationale, French-Speaking Association for the Study of Diabetes and Metabolism (Alfediam), French National Research Agency (ANR), National Institute for Research in Public Health (Institut de Recherche en Sante Publique, the Tres Grande Infrastructure de Recherche Cohorte Sante 2008 Program), Cohort Program, ANR-12-DSSA-0005,DYSEDEN,Déterminants sociaux des troubles psychologiques et des apprentissages chez les enfants d'âge scolaire(2012), de Lauzon-Guillain, Blandine, Déterminants sociaux de santé - Déterminants sociaux des troubles psychologiques et des apprentissages chez les enfants d'âge scolaire - - DYSEDEN2012 - ANR-12-DSSA-0005 - DSS - VALID, Hôpital Charles Perrens, Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA), École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), and Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Conduct Disorder, Male, medicine.medical_specialty, Offspring, conduct problems, 03 medical and health sciences, 0302 clinical medicine, children, Epidemiology, Developmental and Educational Psychology, medicine, Humans, Attention deficit hyperactivity disorder, 030212 general & internal medicine, Child, 2. Zero hunger, Pregnancy, Odds ratio, medicine.disease, externalizing, 3. Good health, Diet, Gestational diabetes, Psychiatry and Mental health, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, attention deficit hyperactivity disorder, Attention Deficit Disorder with Hyperactivity, Diet, Western, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, Child, Preschool, Prenatal Exposure Delayed Effects, Pediatrics, Perinatology and Child Health, Cohort, Gestation, Female, epidemiology, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, France, pregnancy, Diet, Healthy, Psychology, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, 030217 neurology & neurosurgery, mental health, Follow-Up Studies, Demography
Abstract: International audience; Background: Evidence shows that diet contributes substantially to lifelong physical and mental health. Although dietary exposure during gestation and early postnatal life is critical, human epidemiological data are limited regarding its link with children's subsequent externalizing issues. The aim of this study was to investigate the role of maternal diet during pregnancy in offspring's symptoms of hyperactivity-inattention and conduct problems from ages 3 to 8 years. Methods: We used data of 1,242 mother-child pairs from a French cohort followed up from pregnancy until the children were 8 years of age. Dietary patterns (DP) of the mother during pregnancy were assessed with food frequency questionnaires. Children's externalizing behavior was assessed with the Strength and Difficulties Questionnaire at ages 3, 5, and 8 years, from which trajectories of hyperactivity-inattention symptoms and conduct problems were derived. We conducted multivariable logistic models to study associations adjusted for a range of potential confounders. Results: Results showed significant relationships between maternal 'low Healthy diet' (adjusted Odds Ratio (aOR) = 1.61; IC 95%: 1.09-2.37) and 'high Western diet' (aOR=1.67; IC 95%: 1.13-2.47) during pregnancy and children's trajectories of high symptoms of hyperactivity-inattention. The associations took into account relevant confounders such as DP of the children at age 2 years, maternal stress and depression, gestational diabetes, and socioeconomic variables. Conclusions: Maternal diet during pregnancy was independently associated with children's hyperactivity-inattention symptoms but not with conduct problems. Early prevention addressing lifestyle should specifically target diet in pregnant women.
Published: 2018
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31. Cost-Effectiveness Analysis of a Navigation Program for Colorectal Cancer Screening to Reduce Social Health Inequalities: A French Cluster Randomized Controlled Trial

Author: Lydia Guittet, Rémy De Mil, Olivier Dejardin, Véronique Christophe, Elodie Guillaume, Hélène Delattre-Massy, Carole Pornet, Chantal De Seze, Guy Launoy, Jérome Peng, Annick Notari, Célia Berchi, Véronique Bouvier, Unité de recherche interdisciplinaire pour la prévention et le traitement des cancers (ANTICIPE), CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Régional de Lutte contre le Cancer François Baclesse [Caen] (UNICANCER/CRLC), UNICANCER-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-UNICANCER, Agence Régionale de Santé de Normandie [Caen] (ARS Normandie), Sciences Cognitives et Sciences Affectives (SCALab) - UMR 9193 (SCALab), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Hôpital Côte de Nacre [CHU Caen], Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN), Association Dépistage des Maladies dans la Somme (ADEMA 80), Association pour le dépistage des cancers dans l'Oise (ADECASO), Aisne Preventis [Laon], This work was supported by the French National Institute of Cancer (2010-065) and by the Cancéropole Nord-Ouest (2011-098) through a payment convention between the French National Institute of Cancer and the Cancéropole Nord-Ouest from a grant dedicated to the 2009 to 2013 cancer plan (2009-13)., Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN)-Centre Régional de Lutte contre le Cancer François Baclesse [Caen] (UNICANCER/CRLC), Normandie Université (NU)-UNICANCER-Tumorothèque de Caen Basse-Normandie (TCBN)-UNICANCER-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Sciences Cognitives et Sciences Affectives - UMR 9193 (SCALab), Bodescot, Myriam, and UNICANCER-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-UNICANCER-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Male, Cost-Benefit Analysis, Social Workers, colorectal cancer screening, law.invention, Indirect costs, 0302 clinical medicine, Randomized controlled trial, law, Cluster Analysis, Mass Screening, 030212 general & internal medicine, Prospective Studies, Early Detection of Cancer, health care economics and organizations, Social work, Health Policy, Age Factors, cost-effectiveness analysis, Cost-effectiveness analysis, Middle Aged, 3. Good health, patient navigation, 030220 oncology & carcinogenesis, Female, France, Colorectal Neoplasms, medicine.medical_specialty, [SDV.CAN]Life Sciences [q-bio]/Cancer, Disease cluster, 03 medical and health sciences, [SDV.CAN] Life Sciences [q-bio]/Cancer, Intervention (counseling), medicine, Humans, Social inequality, Social determinants of health, Healthcare Disparities, Aged, social inequalities, business.industry, Public Health, Environmental and Occupational Health, Health Status Disparities, Surgery, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, Patient Compliance, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Patient Participation, business, Demography
Abstract: International audience; BACKGROUND:Patient navigation programs to increase colorectal cancer (CRC) screening adherence have become widespread in recent years, especially among deprived populations.OBJECTIVES:To evaluate the cost-effectiveness of the first patient navigation program in France.METHODS:A total of 16,250 participants were randomized to either the usual screening group (n = 8145) or the navigation group (n = 8105). Navigation consisted of personalized support provided by social workers. A cost-effectiveness analysis of navigation versus usual screening was conducted from the payer perspective in the Picardy region of northern France. We considered nonmedical direct costs in the analysis.RESULTS:Navigation was associated with a significant increase of 3.3% (24.4% vs. 21.1%; P = 0.003) in participation. The increase in participation was higher among affluent participants (+4.1%; P = 0.01) than among deprived ones (+2.6%; P = 0.07). The cost per additional individual screened by navigation compared with usual screening (incremental cost-effectiveness ratio) was €1212 globally and €1527 among deprived participants. Results were sensitive to navigator wages and to the intervention effectiveness whose variations had the greatest impact on the incremental cost-effectiveness ratio.CONCLUSIONS:Patient navigation aiming at increasing CRC screening participation is more efficient among affluent individuals. Nevertheless, when the intervention is implemented for the entire population, social inequalities in CRC screening adherence increase. To reduce social inequalities, patient navigation should therefore be restricted to deprived populations, despite not being the most cost-effective strategy, and accepted to bear a higher extra cost per additional individual screened.TRIAL REGISTRATION:ClinicalTrials.gov NCT01555450.
Published: 2018
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32. Mindfulness Is Associated with the Metabolic Syndrome among Individuals with a Depressive Symptomatology

Author: Pilar Galan, Julia Baudry, Erika Guyot, Emmanuelle Kesse-Guyot, Serge Hercberg, Sandrine Péneau, Institut National de la Recherche Agronomique (INRA), Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Avicenne Hosp, Publ Hlth Dept, F-93017 Bobigny, France, Partenaires INRAE, Paris 13 Univ, Sorbonne Paris Cite, Surveillance & Nutr Epidemiol Res Unit, Sante Publ France,COMUE, F-93017 Bobigny, France, Equipe 3: EREN- Equipe de Recherche en Epidémiologie Nutritionnelle (CRESS - U1153), Université Paris 13 (UP13)-Institut National de la Recherche Agronomique (INRA)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), French Ministry of Health (DGS), Sante publique France (SpF), French National Institute for Health and Medical Research (INSERM), French National Institute for Agricultural Research (INRA), National Conservatory for Arts and Crafts (CNAM), Medical Research Foundation (FRM), University of Paris 13, ProdInra, Migration, Institut National de la Recherche Agronomique (INRA)-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Université Paris 13 (UP13)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), and Guyot, Erika
Subjects: Blood Glucose, Male, 050103 clinical psychology, Mindfulness, mindfulness, [SDV]Life Sciences [q-bio], Overweight, 0302 clinical medicine, Surveys and Questionnaires, Epidemiology, Odds Ratio, Medicine, risk factors, Prospective Studies, 030212 general & internal medicine, metabolic syndrome x, Depression (differential diagnoses), Metabolic Syndrome, education.field_of_study, Nutrition and Dietetics, 05 social sciences, Middle Aged, 3. Good health, [SDV] Life Sciences [q-bio], Eating disorders, depression, Female, epidemiology, France, Waist Circumference, medicine.symptom, lcsh:Nutrition. Foods and food supply, Clinical psychology, Adult, medicine.medical_specialty, Waist, Population, lcsh:TX341-641, Article, 03 medical and health sciences, Humans, 0501 psychology and cognitive sciences, education, Aged, Chi-Square Distribution, business.industry, Cholesterol, HDL, medicine.disease, Cross-Sectional Studies, Logistic Models, Multivariate Analysis, Metabolic syndrome, business, Biomarkers, Food Science
Abstract: International audience; The Metabolic Syndrome (MetS) is a major public health burden. Dispositional mindfulness has recently been associated with eating disorders, being overweight, and could therefore be associated with the MetS. We aimed to examine in a cross-sectional design the relationship between mindfulness, the MetS, and its risk factors in a large sample of the adult general population and the influence of depressive symptomatology on this association. Adults participating in the NutriNet-Sante study who had completed the Five Facets Mindfulness Questionnaire and attended a clinical and biological examination were available for inclusion. Multivariable logistic regression models adjusted for socio-demographic and lifestyle factors were performed. A total of 17,490 individuals were included. Among individuals with a depressive symptomatology, those with higher mindfulness were less likely to have a MetS (OR: 0.73, 95% CI: 0.57-0.93), a high waist circumference, a low HDL-cholesterol level and an elevated fasting blood glucose level (all p
Published: 2018
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33. Identification by proteomics of oviductal sperm-interacting proteins

Author: Marie Saint-Dizier, Lucie Combes-Soia, Xavier Druart, Valérie Labas, Guillaume Tsikis, Julie Lamy, Perrine Nogues, Pascal Mermillod, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Plate-forme de Chirurgie et Imagerie pour la Recherche et l’Enseignement (CIRE), Institut National de la Recherche Agronomique (INRA), European Regional Development Fund (ERDF) [3069], Conseil Regional du Centre, French National Institute for Agricultural Research (INRA), French National Institute of Health and Medical Research (Inserm), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Male, Proteomics, Embryology, endocrine system, statistical-model, epithelial-cells, [SDV]Life Sciences [q-bio], fluproteins, Estrous Cycle, Oviducts, Luteal phase, Biology, Andrology, 03 medical and health sciences, Endocrinology, Capacitation, Tandem Mass Spectrometry, OVGP1, Animals, estrus-associated protein, [INFO]Computer Science [cs], estrous-cycle, membrane, reproductive and urinary physiology, Glycoproteins, Estrous cycle, bovine sperm, Sperm-Ovum Interactions, urogenital system, Obstetrics and Gynecology, Cell Biology, Sperm, Spermatozoa, 030104 developmental biology, Reproductive Medicine, shotgun proteomics, Proteome, in-vitro capacitation, Cattle, Female
Abstract: The interactions between oviductal fluid (OF) proteins and spermatozoa play major roles in sperm selection, storage and capacitation before fertilization. However, only a few sperm-interacting proteins in the OF has been identified and very little is known about the regulation of sperm-oviduct interactions across the estrous cycle. Samples of bovine frozen-thawed sperm from three bulls were incubated with OF at pre-, post-ovulatory stages (Pre-/Post-ov) or luteal phase (LP) of the estrous cycle (7 mg/mL proteins, treated groups) or with a protein-free media (control). The proteomes of sperm cells were assessed by nanoLC–MS/MS and quantified by label-free methods. A total of 27 sperm-interacting proteins originating in the OF were identified. Among those, 14 were detected at all stages, eight at Post-ov and LP and five only at LP. The sperm-interacting proteins detected at all stages or at LP and Post-ov were on average more abundant at LP than at other stages (P
Published: 2017
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34. On the turning away

Author: de Brevern, Alexandre, Biologie Intégrée du Globule Rouge (BIGR (UMR_S_1134 / U1134)), Institut National de la Transfusion Sanguine [Paris] (INTS)-Université Paris Diderot - Paris 7 (UPD7)-Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université des Antilles (UA), is work was supported by grants from the French Ministry of Research, University of Paris Diderot – Paris 7, French National Institute for Blood Transfusion (INTS), French Institute for Health and Medical Research (INSERM). AdB also acknowledges the Indo-French Centre for the Promotion of Advanced Research/CEFIPRA for collaborative grants (numbers 3903-E and 5302-2). is study was supported by grants from the Laboratory of Excellence GR-Ex, reference ANR-11-LABX-0051. e labex GR-Ex is funded by the programme 'Investissements d’avenir' of the French National Research Agency, reference ANR-11- IDEX-0005-02. Calculations were performed on an SGI cluster granted by Conseil Régional Ile de France and INTS (SESAME Grant)., de Brevern, Alexandre G., and This work was supported by grants from the French Ministry of Research, University of Paris Diderot – Paris 7, French National Institute for Blood Transfusion (INTS), French Institute for Health and Medical Research (INSERM). AdB also acknowledges the Indo-French Centre for the Promotion of Advanced Research/CEFIPRA for collaborative grants (numbers 3903-E and 5302-2). is study was supported by grants from the Laboratory of Excellence GR-Ex, reference ANR-11-LABX-0051. e labex GR-Ex is funded by the programme 'Investissements d’avenir' of the French National Research Agency, reference ANR-11- IDEX-0005-02. Calculations were performed on an SGI cluster granted by Conseil Régional Ile de France and INTS (SESAME Grant).
Subjects: secondary structures, propensities, structural comparison, k-means, helical structures, classification, Protein Data Bank, Protein Blocks, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, structural alphabet, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, amino acid, protein folds, Self-Organizing Maps
Abstract: National audience; The functional properties of a protein depend mainly on its three-dimensional (3D) structure. They are classically assigned, visualized and analysed through the prism of classical secondary structures composed of repetitive parts (α-helices for 1/3rd of the residues and β-strands for 1/5th, resp.) connected by coil. Two other repetitive structures also exist, namely the PolyProline II and the β-turns.The β-turns have been characterized by a hydrogen bond between N-H and C=O of residues i and i+3 by Venkatachalam [1]. He also characterized the first β-turn types. Later novel turns were defined, some being discarded, leading to a final collection of type I, I’, II, II’, IV, VIa1, VIa2, VIb, and VIII β-turns. Types VIa1, VIa2 and VIb are characterized by the presence of a cis-Proline at residue i+2. Turns that do not fit any of the above criteria are classified as type IV [2].β-turn IV, i.e. the miscellaneous category, represents near 1/3rd of β-turn residues in protein structure, and is the second most frequent β -turn. 25 years have passed since the last proposition of an extension of the classical definition of β-turns. Over all we have known, it seems a good moment to dig them and to see if some new recurrent conformations are not hidden into this miscellaneous type. An automatic clustering approach based on the rules of β-turn type assignment was designed to search for recurrent new turns inside this miscellaneous type. The four most occurring clusters defined the new β-turn types. Surprisingly, these types, named IV1, IV2, IV3 and IV4, represent half of the type IV β-turns, and are more frequent that many established ones. Type IV1, is in the neighbourhood of type II but with very different amino acid composition, while IV2 is close to type VIII with related amino acid content. Types IV3 and IV4 are in the same dihedral angle region than frequent β-turn type I, but with distinct dihedral angle values [3].[1] Venkatachalam CM (1968) Stereochemical criteria for polypeptides and proteins. V. Conformation of a system of three linked peptide units. Biopolymers 6:1425-1436.[2] Hutchinson EG, Thornton JM (1996) PROMOTIF--a program to identify and analyze structural motifs in proteins. Protein Sci 5:212-220.[3] de Brevern AG (2016) Extension of the classical classification of β-turns. Sci Rep. 2016 Sep 15;6:33191.
Published: 2017

35. Influence of infant feeding patterns over the first year of life on growth from birth to 5 years: Feeding patterns and growth in early childhood

Author: M.-A. Charles, Barbara Heude, Sophie Carles, Aisha Betoko, B. de Lauzon-Guillain, Régis Hankard, Sandrine Lioret, Jérémie Botton, A. Forhan, N. Regnault, Equipe 6 : ORCHAD - Origines précoces de la santé du développement de l'enfant (CRESS - U1153), Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA), Nutrition, croissance et cancer (U 1069) (N2C), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Francois Rabelais [Tours], Faculté de Pharmacie, Université Paris-Sud - Paris 11 (UP11), We acknowledge all funding sources for the EDEN study: Fondation pour la Recherche Médicale (FRM), French Ministry of Research: Federative Research Institutes and Cohort Program, INSERM Human Nutrition National Research Program, and Diabetes National Research Program (through a collaboration with the French Association of Diabetic Patients (AFD)), French Ministry of Health, French Agency for Environment Security (AFSSET), French National Institute for Population Health Surveillance (InVS), Paris–Sud University, French National Institute for Health Education (INPES), Nestlé, Mutuelle Générale de l’Education Nationale (MGEN), French speaking association for the study of diabetes and metabolism (ALFEDIAM), National Agency for Research (ANR non thematic program), National Institute for Research in Public health (IRESP: TGIR cohorte santé 2008 program). The research leading to these results has received funding from the European Community's Seventh Framework Program (FP7/ 2007-2013) under the grant agreement n°FP7-245012- HabEat and from the National Agency for Research (ANR Social determinants of health program) under the grant agreement n°ANR-12-DSSA-0001 (SOFI project). Aisha Betoko was supported by a research grant from the French Ministry for Higher Education and Research., The EDEN mother-child cohort study group, ANR-12-DSSA-0001,SOFI,Déterminants socio-culturel des pratiques alimentaires dans l'enfance(2012), European Project: 245012,EC:FP7:KBBE,FP7-KBBE-2009-3,HABEAT(2010), Université Paris Descartes - Paris 5 (UPD5)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Tours, Université Paris-Sud - Paris 11 (UP11)-Université Paris-Saclay, Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: preschool children, growth, Breastfeeding, 030209 endocrinology & metabolism, 03 medical and health sciences, 0302 clinical medicine, medicine, 030212 general & internal medicine, Infant feeding, 2. Zero hunger, Meal, Nutrition and Dietetics, business.industry, Health Policy, Confounding, Public Health, Environmental and Occupational Health, Anthropometry, medicine.disease, Obesity, Pediatrics, Perinatology and Child Health, Cohort, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, business, Body mass index, feeding patterns, Birth cohort, Demography
Abstract: International audience; OBJECTIVES: As early-life feeding experiences may influence later health, we aimed to examine relations between feeding patterns over the first year of life and child's growth in the first 5 years of life. METHODS: Our analysis included 1022 children from the EDEN mother-child cohort. Three feeding patterns were previously identified, i.e. 'Later dairy products introduction and use of ready-prepared baby foods' (pattern-1), 'Long breastfeeding, later main meal food introduction and use of home-made foods' (pattern-2) and 'Use of ready-prepared adult foods' (pattern-3). Associations between the feeding patterns and growth [weight, height and body mass index {BMI}] were analysed by multivariable linear regressions. Anthropometric changes were assessed by the final value adjusted for the initial value. RESULTS: Even though infant feeding patterns were not related to anthropometric measurements at 1, 3 and 5 years, high scores on pattern-1 were associated with higher 1-3 years weight and height changes. High scores on pattern-2 were related to lower 0-1 year weight and height changes, higher 1-5 years weight and height changes but not to BMI changes, after controlling for a wide range of potential confounding variables including parental BMI. Scores on pattern-3 were not significantly related to growth. Additional adjustment for breastfeeding duration reduced the strength of the associations between pattern-2 and growth but not those between pattern-1 and height growth. CONCLUSION: Our findings emphasize the relevance of considering infant feeding patterns including breastfeeding duration, age of complementary foods introduction as well as type of foods used when examining effects of early infant feeding practices on later health. (c) 2017 World Obesity Federation.
Published: 2017
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36. Qualitative proteomic analysis of Tipula oleracea nudivirus occlusion bodies

Author: Annie Bézier, Grégoire Harichaux, Karine Musset, Elisabeth A. Herniou, Valérie Labas, UMR 7261, Centre National de la Recherche Scientifique (CNRS), Université Francois Rabelais [Tours], Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Plateforme d'Analyse Intégrative des Biomolécules, Institut National de la Recherche Agronomique (INRA), (SMHART project) by the European Regional Development Fund (ERDF), Conseil Régional du Centre, French National Institute for Agricultural Research (INRA), French National Institute of Healt Medical Research (Inserm), European Project: 206205, Institut de recherche sur la biologie de l'insecte UMR7261 (IRBI), Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Université de Tours-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Proteomics, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], food.ingredient, nudiviris, proteome, Virologie, Hypothetical protein, Protein Array Analysis, Mass Spectrometry, Nudivirus, virus à adn, 03 medical and health sciences, Viral Proteins, food, Penaeidae, Virology, analyse qualitative, Polyhedrin, qualitative analysis, Animals, protéomique, électrophorèse, Arthropods, biology, DNA Viruses, biology.organism_classification, Molecular biology, bracovirus, 3. Good health, 030104 developmental biology, Capsid, electrophoresis, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Proteome, Insect Proteins, Tipula oleracea, Electrophoresis, Polyacrylamide Gel, DNA, Circular, Bracovirus, Baculoviridae, nucléopolyhédrovirus, Autre (Sciences du Vivant), Chromatography, Liquid
Abstract: Nudiviruses are arthropod-specific large double-stranded circular DNA viruses, related to baculoviruses, which replicate in the nucleus of the cells they infect. To date, six fully sequenced nudiviral genomes are available in databases, and the protein profile from nudivirus particles was mainly characterized by PAGE. However, only a few direct matches have been completed between genomic and proteomic data, with the exception of the major occlusion body protein from Penaeus monodon nudivirus and four nucleocapsid proteins from Helicoverpa zea nudivirus-2. The function of predicted nudiviral proteins is still inferred from what is known from baculoviruses or endogenous nudiviruses (i.e. bracoviruses). Tipula oleracea nudivirus (ToNV) is the causative agent of crane fly nucleopolyhedrosis. Along with Penaeus monodon nudivirus, ToNV is the second fully sequenced nudivirus to be described as forming occlusion bodies. The protein profile revealed by Coomassie-stained SDS-PAGE is very similar to those observed for other nudiviruses, with five major protein bands of about 75, 48, 35, 25 and 12 kDa. Proteomic analysis, using on-line nanoflow liquid chromatography in tandem with high-resolution mass spectrometry, revealed that ToNV occlusion bodies are composed of 52 viral proteins, the most abundant of which are the functional homologue of baculovirus polyhedrin/granulin and the homologues of three Helicoverpa zea nudivirus-2 predicted proteins: the two virion structural proteins 34K (Hz2V052, the baculovirus capsid protein VP39 homologue) and 11K (Hz2V025), and the hypothetical protein Hz2V079, a newly identified nudivirus core gene product.
Published: 2017
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37. Meal planning is associated with food variety, diet quality and body weight status in a large sample of French adults

Author: Gladys Ibanez, Benjamin Allès, Pauline Ducrot, Emmanuelle Kesse-Guyot, Serge Hercberg, Sandrine Péneau, Vani Aroumougame, Caroline Méjean, Equipe 3: EREN- Equipe de Recherche en Epidémiologie Nutritionnelle (CRESS - U1153), Université Paris 13 (UP13)-Institut National de la Recherche Agronomique (INRA)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Pierre et Marie Curie - Paris 6 - UFR de Médecine Pierre et Marie Curie (UPMC), Université Pierre et Marie Curie - Paris 6 (UPMC), Dpt Santé publique [CHU Avicenne], Hôpital Avicenne [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), French Ministry of Health (DGS), French Institute for Public Health Surveillance (InVS), French National Institute for Health and Medical Research (INSERM), French National Institute for Agricultural Research (INRA), Medical Research Foundation (FRM), National Conservatory for Arts and Crafts (CNAM), National Institute for Prevention and Health Education (INPES), University of Paris, HAL UPMC, Gestionnaire, Institut National de la Recherche Agronomique (INRA)-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Université Paris 13 (UP13)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Université Paris 13 (UP13)-Institut National de la Recherche Agronomique (INRA)-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Hôpital Avicenne, and Ducrot, Pauline
Subjects: 0301 basic medicine, Adult, Male, Adolescent, Cross-sectional study, Medicine (miscellaneous), Physical Therapy, Sports Therapy and Rehabilitation, Clinical nutrition, Meal planning, Food variety, Overweight, Diet quality, Body Mass Index, Food group, 03 medical and health sciences, Young Adult, Environmental health, medicine, Humans, Cooking, Obesity, Meals, Aged, 2. Zero hunger, Meal, 030109 nutrition & dietetics, Nutrition and Dietetics, business.industry, Research, Body Weight, digestive, oral, and skin physiology, Feeding Behavior, Middle Aged, medicine.disease, Diet Records, 3. Good health, Diet, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, Cross-Sectional Studies, Quartile, Meal preparation, Female, medicine.symptom, business, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract: Background Meal planning could be a potential tool to offset time scarcity and therefore encourage home meal preparation, which has been linked with an improved diet quality. However, to date, meal planning has received little attention in the scientific literature. The aim of our cross-sectional study was to investigate the association between meal planning and diet quality, including adherence to nutritional guidelines and food variety, as well as weight status. Methods Meal planning, i.e. planning ahead the foods that will be eaten for the next few days, was assessed in 40,554 participants of the web-based observational NutriNet-Santé study. Dietary measurements included intakes of energy, nutrients, food groups, and adherence to the French nutritional guidelines (mPNNS-GS) estimated through repeated 24-h dietary records. A food variety score was also calculated using Food Frequency Questionnaire. Weight and height were self-reported. Association between meal planning and dietary intakes were assessed using ANCOVAs, while associations with quartiles of mPNNS-GS scores, quartiles of food variety score and weight status categories (overweight, obesity) were evaluated using logistic regression models. Results A total of 57% of the participants declared to plan meals at least occasionally. Meal planners were more likely to have a higher mPNNS-GS (OR quartile 4 vs. 1 = 1.13, 95% CI: [1.07–1.20]), higher overall food variety (OR quartile 4 vs. 1 = 1.25, 95% CI: [1.18–1.32]). In women, meal planning was associated with lower odds of being overweight (OR = 0.92 [0.87–0.98]) and obese (OR = 0.79 [0.73–0.86]). In men, the association was significant for obesity only (OR = 0.81 [0.69–0.94]). Conclusions Meal planning was associated with a healthier diet and less obesity. Although no causality can be inferred from the reported associations, these data suggest that meal planning could potentially be relevant for obesity prevention. Electronic supplementary material The online version of this article (doi:10.1186/s12966-017-0461-7) contains supplementary material, which is available to authorized users.
Published: 2017
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38. Early factors related to carbohydrate and fat intake at 8 and 12 months: results from the EDEN mother–child cohort

Author: Christine Lange, M.-A. Charles, W L Yuan, Sophie Nicklaus, A. Forhan, Barbara Heude, B. de Lauzon-Guillain, Sandrine Lioret, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Dietary Survey Unit Nutr Epidemiol OCA EN, Agence Française de Sécurité Sanitaire des Aliments, Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Didit metaprogramme of INRA Foundation for Medical Research (FRM) National Agency for Research (ANR) National Institute for Research in Public Health (IRESP: TGIR cohorte sante programme) French Ministry of Health (DGS) French Ministry of Research, Inserm Bone and Joint Diseases National Research (PRO-A) Human Nutrition National Research Programmes, Paris-Sud University Nestle French National Institute for Population Health Surveillance (InVS) French National Institute for Health Education (INPES) European Union FP7 programmes (FP7, HELIX, ESCAPE, ENRIECO, Medall projects) Diabetes National Research Programme (French Association of Diabetic Patients (AFD) ANSES Mutuelle Generale de l'Education Nationale (MGEN) French National Agency for Food Security French-speaking association for the study of diabetes and metabolism (ALFEDIAM), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Institut National de la Santé et de la Recherche Médicale ( INSERM ), Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité ( CRESS (U1153 / UMR_A 1125) ), Institut National de la Recherche Agronomique ( INRA ) -Université Sorbonne Paris Cité ( USPC ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), and Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)
Subjects: 0301 basic medicine, Vitamin, Adult, multivariate-analysis, Calorie, [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, dietary patterns, Medicine (miscellaneous), Physiology, Recommended Dietary Allowances, Cohort Studies, 03 medical and health sciences, chemistry.chemical_compound, Eating, Fat intake, medicine, Dietary Carbohydrates, Humans, Food science, Infant Nutritional Physiological Phenomena, 2. Zero hunger, 030109 nutrition & dietetics, Nutrition and Dietetics, business.industry, infants, Infant, eating habits, Feeding Behavior, Maternal Nutritional Physiological Phenomena, food-intake, Carbohydrate, medicine.disease, Obesity, Dietary Fats, 3. Good health, Diet, Breast Feeding, chemistry, age, principal-components, Cohort, Female, sociodemographic characteristics, business, Energy Intake, energy-intake, Body mass index, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, associations
Abstract: International audience; BACKGROUND/OBJECTIVES: Few studies have examined the factors explaining the variability in fat and carbohydrate intake during infancy. We aimed to describe infants' fat and carbohydrate intake and analyse the associations with infant and maternal characteristics and feeding practices.SUBJECTS/METHODS: This study included 1275 infants aged 8 months from the French EDEN mother-child cohort. Carbohydrate intake, fat intake, added fat (vegetable oils and animal fats) and added sugar (honey, white sugar, brown sugar, jam and sweetened beverages) consumption were calculated at 8 and 12 months. Associations between these variables and infant and maternal characteristics as well as maternal dietary patterns during pregnancy, breast-feeding duration and age at complementary feeding introduction were analysed using multivariable linear and logistic regressions.RESULTS: Less than 5% of non-breast-fed infants reached the recommendation of consuming at least 40% of total energy from fat, whereas more than 95% of them reached 45% of energy from carbohydrates. Overall, infant and maternal characteristics and maternal diet during pregnancy were marginally associated with both carbohydrate/added sugar and fat/added fat intake. Nevertheless, age at complementary feeding introduction was associated with all outcomes.CONCLUSIONS: Our results suggest that only a small proportion of non-breast-fed infants at 8 and 12 months reached the recommendations for fat intake, whereas a majority of them reached the recommendations for carbohydrate intake. As subgroups of infants with a higher risk of inadequate diet were not identified, the present results call for an improved dissemination of information regarding infant-specific dietary fat needs in the entire population.
Published: 2017
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39. Investigating proteins and proteases composing amniotic and allantoic fluids during chicken embryonic development

Author: Yves Nys, Sophie Réhault-Godbert, Valérie Labas, M. Da Silva, Recherches Avicoles (SRA), Institut National de la Recherche Agronomique (INRA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Plateforme d'Analyse Intégrative des Biomolécules, (SMHART project) by the European Regional Development Fund (ERDF), the Conseil Régional du Center, the French National Institute for Agricultural Research (INRA), French National Institute of Health and Medical Research (Inserm), Unité de Recherches Avicoles (URA), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Proteases, proteolysis, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], food.ingredient, Amniotic fluid, Metal ion transport, medicine.medical_treatment, chicken, mass spectrum analysis, poulet, Context (language use), Chick Embryo, Biology, proteolyse, Mass Spectrometry, Avian Proteins, 03 medical and health sciences, food, Allantois, spectrométrie de masse, Yolk, medicine, Animals, foetal development, Protease, Meprin A, chicken egg, allantoic fluid, 0402 animal and dairy science, Proteolytic enzymes, amniotic fluid, protease, 04 agricultural and veterinary sciences, General Medicine, 040201 dairy & animal science, Molecular biology, 030104 developmental biology, Biochemistry, développement embryonnaire, embryonic development, Electrophoresis, Polyacrylamide Gel, Animal Science and Zoology, Chickens, Peptide Hydrolases
Abstract: In amniotes, the amniotic fluid is a significant contributor to fetal development and health. While numerous studies have been conducted in mammalian amniotic fluid, the composition of amniotic and other extraembryonic fluids in avian egg along with their physiological functions remain largely unexplored. In such a context, our objective was to characterize the chicken amniotic fluid (AmF) and allantoic fluid (AlF) properties, protein composition, and some associated functions from day 8 to day 16 of incubation. SDS-PAGE combined to mass spectrometry analysis revealed common and specific proteins to each fluid, suggesting distinct properties and functions. Indeed, major AlF proteins are mostly “egg yolk” proteins involved in lipid, vitamin metabolisms, and metal ion transport, while major AmF proteins resemble those of albumen. Drastic changes in the AmF protein profiles were observed during incubation, when the albumen transfers from day 12 onwards, while few changes were detected for the AlF protein profile. The decreases in osmolality (from 231 to 183 mOsm/kg) and pH (from 8.26 to 7.26) observed in the AlF during incubation are associated with water and electrolytes reallocation for the embryo needs. In contrast, AmF pH value remained stable (≈7.5). Active proteolytic enzymes have been identified in the 2 fluids using gelatin zymography, followed by mass spectrometry analysis for protease identification. A total of 12 proteases was detected in the AlF, compared to 5 in the AmF. We have shown that AlF concentrates proteolytic enzymes assumed to participate in digestive processes: aminopeptidase N, dipeptidyl peptidase-4, meprin A, and 72 kDa type IV collagenase preproprotein. The other proteases identified in both fluids also could have a role in morphogenesis (hepatocyte growth factor activator, suppressor of tumorigenicity 14, astacin-like metalloendopeptidase) and hemostasis (prothrombin and coagulation factor X). Altogether, these data suggest that the roles of chicken AlF and AmF are not merely associated with protection of the embryo and regulation of metabolic disposable wastes, but also they could have more sophisticated roles during embryonic development.
Published: 2017
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40. Data on endogenous bovine ovarian follicular cells peptides and small proteins obtained through Top-down High Resolution Mass Spectrometry

Author: Laura Bouguereau, Svetlana Uzbekova, Aurélie Marestaing, Valérie Labas, Lucie Spina, Ana-Paula Teixeira-Gomes, Audrey Gargaros, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Plateforme d'Analyse Intégrative des Biomolécules, Institut National de la Recherche Agronomique (INRA), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), European Regional Development Fund (ERDF), Val-de-Loire Region general Committee, French National Institute for Agricultural Research (INRA), French National Institute of Health and Medical Research (INSERM) under the SMHART (LTQ Velos Orbitrap high resolution mass spectrometer acquisition, no 3069) project, INRA, French National Research Agency under grant number ANR-08-GENN-03, Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Infectiologie Animale et Santé Publique - IASP (Nouzilly, France), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Physiologie de la reproduction et des comportements [Nouzilly] ( PRC ), Institut National de la Recherche Agronomique ( INRA ) -Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique ( CNRS ), Institut National de la Recherche Agronomique ( INRA ), Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés ( LISBP ), Institut National de la Recherche Agronomique ( INRA ) -Institut National des Sciences Appliquées - Toulouse ( INSA Toulouse ), and Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -Centre National de la Recherche Scientifique ( CNRS )
Subjects: 0301 basic medicine, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], Follicular cells, bovin, Top-down proteomics, Bovine, Ovary, mass spectrum analysis, Endogeny, Peptide, Biology, maturation ovocytaire, Mass spectrometry, Bioinformatics, lcsh:Computer applications to medicine. Medical informatics, 03 medical and health sciences, spectrométrie de masse, Follicular phase, medicine, [ SDV.OT ] Life Sciences [q-bio]/Other [q-bio.OT], lcsh:Science (General), Data Article, chemistry.chemical_classification, Multidisciplinary, ovaire, Intact cell, Oocyte, peptide, 030104 developmental biology, medicine.anatomical_structure, Biochemistry, Functional annotation, chemistry, lcsh:R858-859.7, ovary, Autre (Sciences du Vivant), lcsh:Q1-390
Abstract: The endogenous peptides and small proteins extracted from bovine ovarian follicular cells (oocytes, cumulus and granulosa cells) were identified by Top-down High Resolution Mass Spectrometry (TD-HR-MS/MS) in order to annotate peptido- and proteoforms detected using qualitative and quantitative profiling method based on ICM-MS (Intact Cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry). The description and analysis of these Top-down MS data in the context of oocyte quality biomarkers research are available in the original research article of Labas et al. (2017) http://dx.doi.org/10.1016/j.jprot.2017.03.027 [1]. Raw data derived from this peptidomic/proteomic analysis have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository (dataset identifier PXD004892). Here, we described the inventory of all identified peptido- and proteoforms including their biochemical and structural features, and functional annotation of correspondent proteins. This peptide/protein inventory revealed that TD-HR-MS/MS was appropriate method for both global and targeted proteomic analysis of ovarian tissues, and it can be further employed as a reference for other studies on follicular cells including single oocytes. Keywords: Top-down proteomics, Bovine, Ovary, Follicular cells
Published: 2017
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41. Associations between motives for dish choice during home-meal preparation and diet quality in French adults: findings from the NutriNet-Santé study

Author: Caroline Méjean, Philippine Fassier, Serge Hercberg, Benjamin Allès, Sandrine Péneau, Pauline Ducrot, Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC), Université Sorbonne Paris Cité (USPC), Department of Public Health, Vientiane Municipality, French Ministry of Health, French Institute for Public Health Surveillance, French National Institute for Health and Medical Research, French National Institute for Agricultural Research, Medical Research Foundation, National Conservatory for Arts and Crafts, National Institute for Prevention and Health Education, University of Paris 13, Open Food System project, Vitagora, Cap Digital, Imaginove, Aquimer, Microtechnique, Agrimip - French State, Agrimip - Franche-Comte Region, Investments for the Future Programme, Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS), Institut National de la Recherche Agronomique (INRA) - Université Paris Diderot - Paris 7 (UPD7) - Université Paris Descartes - Paris 5 (UPD5) - Université Paris 13 - Institut National de la Santé et de la Recherche Médicale (INSERM), and Université Sorbonne Paris Cité (COMUE) (USPC)
Subjects: 0301 basic medicine, Adult, Male, food.type_of_dish, Convenience food, Adolescent, Cross-sectional study, Food Handling, media_common.quotation_subject, [SDV]Life Sciences [q-bio], Medicine (miscellaneous), home-meal preparation, Logistic regression, Choice Behavior, Diet Surveys, Pleasure, Nutrition Policy, Food group, 03 medical and health sciences, Food Preferences, Young Adult, food, Environmental health, Medicine, Humans, Food science, media_common, Aged, 2. Zero hunger, Analysis of Variance, Motivation, 030109 nutrition & dietetics, Nutrition and Dietetics, business.industry, diet quality, Middle Aged, Diet, [SDV] Life Sciences [q-bio], Logistic Models, Diet quality, dish choices, Cross-sectional studies, Meal preparation, Causal link, Female, France, Diet, Healthy, business, Attitude to Health
Abstract: A number of motives such as constraints or pleasure have been suggested to influence dish choices during home-meal preparation. However, no study has evaluated how the importance conferred to these motives potentially influence diet quality. The present study aims at investigating the difference in diet quality according to the importance attached by individuals to various dish choice motives. The importance of twenty-seven criteria related to dish choices on weekdays was evaluated among 48 010 French adults from the NutriNet-Santé study. ANCOVA and logistic regression models, adjusted for sociodemographic and lifestyle factors, were used to evaluate the association between the importance attached to dish choice motives (yes v. no) and energy and food group intakes, as well as adherence to French nutritional guidelines (modified Programme National Nutrition Santé-Guideline Score (mPNNS-GS)). A higher adherence to nutritional guidelines was observed in individuals attaching importance to a healthy diet (mPNNS-GS score 7·87 (sd 0·09) v. 7·39 (sd 0·09)) and specific diets (mPNNS-GS score 7·73 (sd 0·09) v. 7·53 (sd 0·09)), compared with those who attached little/no importance (all PPconstraints, pleasure and organisation, only small differences were observed. The main difference in diet quality was related to the importance placed on a healthy diet. Although a causal link should be demonstrated, our findings suggested that strategies aiming at enabling people to take into account diet quality during home-meal preparation might be effective levers to promote healthy eating.
Published: 2017
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42. Intact cell MALDI-TOF mass spectrometry on single bovine oocyte and follicular cells combined with top-down proteomics: A novel approach to characterise markers of oocyte maturation

Author: Laura Bouguereau, Audrey Gargaros, Aurélie Marestaing, Valérie Labas, Ana-Paula Teixeira-Gomes, Lucie Spina, Svetlana Uzbekova, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Plateforme d'Analyse Intégrative des Biomolécules, Institut National de la Recherche Agronomique (INRA), Infectiologie Animale et Santé Publique - IASP (Nouzilly, France), Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), European Regional Development Fund (ERDF), Val-de-Loire Region general Committee, the French National Institute for Agricultural Research (INRA), French National Institute of Health and Medical Research (INSERM) under the SMHART (LTQ Velos Orbitrap high resolution mass spectrometer acquisition, no. 3069), InnovoMass (ICM-MS) projects, INRA, French National Research Agency., Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Proteomics, bovin, cumulus cells, mass spectrum analysis, Peptide, Meiotic maturation, MALDI-TOF mass spectrometry, Top-down proteomics, oocyte, bovine, 01 natural sciences, Biochemistry, Ubiquitin, Ovarian Follicle, spectrométrie de masse, Follicular phase, Biologie de la reproduction, maturation de l'ovocyte, chemistry.chemical_classification, Reproductive Biology, ovocyte, Cell biology, Meiosis, medicine.anatomical_structure, Female, Single-Cell Analysis, follicule, Autre (Sciences du Vivant), Proteases, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], Biophysics, Biology, Mass spectrometry, reproduction, 03 medical and health sciences, Histone H2B, medicine, Animals, Granulosa Cells, 010401 analytical chemistry, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, Oocyte, Molecular biology, 0104 chemical sciences, 030104 developmental biology, chemistry, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, biology.protein, Oocytes, Cattle, Biomarkers
Abstract: International audience; Intact cell MALDI-TOF mass spectrometry (ICM-MS) was adapted to bovine follicular cells from individual ovarian follicles to obtain the protein/peptide signatures (< 17 kDa) of single oocytes, cumulus cells (CC) and granulosa cells (GC), which shared a total of 439 peaks. By comparing the ICM-MS profiles of single oocytes with CC before and after in vitro maturation (IVM), 71 different peaks were characterised, and their relative abundance was found to vary depending on the stage of oocyte meiotic maturation. To identify these endogenous biomolecules, top-down workflow using high resolution MS/MS (TD HR-MS) was performed on the protein extracts from oocytes, CC and GC. The TD HR-MS proteomic approach allowed for: (1) identification of 386 peptide/proteoforms encoded by 194 genes; and (2) characterisation of proteolysis products likely resulting from the action of kallikreins and caspases. In total, 136 peaks observed by ICM-MS were annotated by TD HR-MS (ProteomeXchange PXD004892). Among these, 16 markers of maturation were identified, including IGF2 binding protein 3 and hemoglobin B in the oocyte, thymosins beta-4 and beta-10, histone H2B and ubiquitin in CC. The combination of ICM-MS and TD HR-MS proved to be a suitable strategy to identify non-invasive markers of oocyte quality using limited biological samples. Biological significance Intact cell MALDI-TOF mass spectrometry on single oocytes and their surrounding cumulus cells, coupled to an optimised top-down HR-MS proteomic approach on ovarian follicular cells, was used to identify specific markers of oocyte meiotic maturation represented by whole low molecular weight proteins or products of degradation by specific proteases.
Published: 2017
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43. Influence of infant feeding patterns over the first year of life on growth from birth to 5 years

Author: Betoko, Aisha, Lioret, Sandrine, Heude, Barbara, Hankard, Regis, Carles, Sophie, Forhan, Anne, Regnault, Nolwenn, Botton, Jérémie, Charles, Marie, de Lauzon-Guillain, Blandine, de Lauzon-Guillain, Blandine, Déterminants sociaux de santé - Déterminants socio-culturel des pratiques alimentaires dans l'enfance - - SOFI2012 - ANR-12-DSSA-0001 - DSS - VALID, Determining factors and critical periods in food habit formation and breaking in early childhood: a multidisciplinary approach - HABEAT - - EC:FP7:KBBE2010-01-01 - 2014-04-30 - 245012 - VALID, Equipe 6 : ORCHAD - Origines précoces de la santé du développement de l'enfant (CRESS - U1153), Université Paris Descartes - Paris 5 (UPD5)-Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA)-Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Nutrition, croissance et cancer (U 1069) (N2C), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Francois Rabelais [Tours], Faculté de Pharmacie, Université Paris-Sud - Paris 11 (UP11), We acknowledge all funding sources for the EDEN study: Fondation pour la Recherche Médicale (FRM), French Ministry of Research: Federative Research Institutes and Cohort Program, INSERM Human Nutrition National Research Program, and Diabetes National Research Program (through a collaboration with the French Association of Diabetic Patients (AFD)), French Ministry of Health, French Agency for Environment Security (AFSSET), French National Institute for Population Health Surveillance (InVS), Paris–Sud University, French National Institute for Health Education (INPES), Nestlé, Mutuelle Générale de l’Education Nationale (MGEN), French speaking association for the study of diabetes and metabolism (ALFEDIAM), National Agency for Research (ANR non thematic program), National Institute for Research in Public health (IRESP: TGIR cohorte santé 2008 program). The research leading to these results has received funding from the European Community's Seventh Framework Program (FP7/ 2007-2013) under the grant agreement n°FP7-245012- HabEat and from the National Agency for Research (ANR Social determinants of health program) under the grant agreement n°ANR-12-DSSA-0001 (SOFI project). Aisha Betoko was supported by a research grant from the French Ministry for Higher Education and Research., The EDEN mother-child cohort study group, ANR-12-DSSA-0001,SOFI,Déterminants socio-culturel des pratiques alimentaires dans l'enfance(2012), and European Project: 245012,EC:FP7:KBBE,FP7-KBBE-2009-3,HABEAT(2010)
Subjects: preschool children, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, growth, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, feeding patterns, Birth cohort
Abstract: International audience; OBJECTIVES: As early-life feeding experiences may influence later health, we aimed to examine relations between feeding patterns over the first year of life and child's growth in the first 5 years of life. METHODS: Our analysis included 1022 children from the EDEN mother-child cohort. Three feeding patterns were previously identified, i.e. 'Later dairy products introduction and use of ready-prepared baby foods' (pattern-1), 'Long breastfeeding, later main meal food introduction and use of home-made foods' (pattern-2) and 'Use of ready-prepared adult foods' (pattern-3). Associations between the feeding patterns and growth [weight, height and body mass index {BMI}] were analysed by multivariable linear regressions. Anthropometric changes were assessed by the final value adjusted for the initial value. RESULTS: Even though infant feeding patterns were not related to anthropometric measurements at 1, 3 and 5 years, high scores on pattern-1 were associated with higher 1-3 years weight and height changes. High scores on pattern-2 were related to lower 0-1 year weight and height changes, higher 1-5 years weight and height changes but not to BMI changes, after controlling for a wide range of potential confounding variables including parental BMI. Scores on pattern-3 were not significantly related to growth. Additional adjustment for breastfeeding duration reduced the strength of the associations between pattern-2 and growth but not those between pattern-1 and height growth. CONCLUSION: Our findings emphasize the relevance of considering infant feeding patterns including breastfeeding duration, age of complementary foods introduction as well as type of foods used when examining effects of early infant feeding practices on later health. (c) 2017 World Obesity Federation.
Published: 2017
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44. Oestrus synchronisation and superovulation alter the cervicovaginal mucus proteome of the ewe

Author: Simon P. de Graaf, Lucie Combes-Soia, Naomi C. Bernecic, Guillaume Tsikis, Clément Soleilhavoup, J.P. Rickard, T. Leahy, Jessie W. Maddison, Grégoire Harichaux, Xavier Druart, Valérie Labas, Faculty of Veterinary Science, University of Sydney, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Plateforme d'Analyse Intégrative des Biomolécules, Institut National de la Recherche Agronomique (INRA), NSW Stud Merino Breeders Association Trust and Australian Wool Innovation, (SMHART project) by the European Regional Development Fund (ERDF), Conseil Régional du Centre, French National Institute for Agricultural Research (INRA), French National Institute of Health and Medical Research (INSERM), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, medicine.medical_specialty, endocrine system, Proteome, medicine.medical_treatment, media_common.quotation_subject, Biophysics, Cervix Uteri, Biology, Luteal phase, Biochemistry, Andrology, 03 medical and health sciences, 0302 clinical medicine, Estrus, ovin, mucus, Internal medicine, Follicular phase, medicine, Animals, Ovulation, protéome, Sperm motility, media_common, 2. Zero hunger, Estrous cycle, superovulation, 030219 obstetrics & reproductive medicine, Sheep, hormones, Artificial insemination, gimmers, brebis, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, exogenous, Mucus, Embryo transfer, cervicovaginal, 030104 developmental biology, Endocrinology, Vagina, oestrous cycle, Female
Abstract: Although essential for artificial insemination (AI) and MOET (multiple ovulation and embryo transfer), oestrus synchronisation and superovulation are associated with increased female reproductive tract mucus production and altered sperm transport. The effects of such breeding practices on the ovine cervicovaginal (CV) mucus proteome have not been detailed. The aim of this study was to qualitatively and quantitatively investigate the Merino CV mucus proteome in naturally cycling (NAT) ewes at oestrus and mid-luteal phase, and quantitatively compare CV oestrus mucus proteomes of NAT, progesterone synchronised (P4) and superovulated (SOV) ewes. Quantitative analysis revealed 60 proteins were more abundant during oestrus and 127 were more abundant during the luteal phase, with 27 oestrus specific and 40 luteal specific proteins identified. The oestrus proteins most disparate in abundance compared to mid-luteal phase were ceruloplasmin (CP), chitinase-3-like protein 1 (CHI3L1), clusterin (CLU), alkaline phosphatase (ALPL) and mucin-16 (MUC16). Exogenous hormones greatly altered the proteome with 51 and 32 proteins more abundant and 98 and 53 proteins less abundant, in P4 and SOV mucus, respectively when compared to NAT mucus. Investigation of the impact of these proteomic changes on sperm motility and longevity within mucus may help improve sperm transport and fertility following cervical AI. Significance This manuscript is the first to detail the proteome of ovine cervicovaginal mucus using qualitative and quantitative proteomic methods over the oestrous cycle in naturally cycling ewes, and also after application of common oestrus synchronisation and superovulation practices. The investigation of the mucus proteome throughout both the follicular and luteal periods of the oestrous cycle, and also after oestrous synchronisation and superovulation provides information about the endocrine control and the effects that exogenous hormones have on protein expression in the female reproductive tract. This information contributes to the field by providing important information on the changes that occur to the cervicovaginal mucus proteome after use of exogenous hormones in controlled breeding programs, which are commonly used on farm and also in a research setting.
Published: 2017
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45. Crossed clinical features between eating disorders and types of bipolar disorder: Results from the FondaMental Advanced Centers of Expertise - Bipolar Disorder cohort

Author: Flaudias, Valentin, Samalin, Ludovic, Godin, Ophélia, Gard, Sébastien, Brousse, Georges, Loftus, Joséphine, Aubin, Valérie, Belzeaux, Raoul, Dubertret, Caroline, Le Strat, Yann, Mazer, Nicolas, de Prémorel, Alix, Roux, Paul, Polosan, Mircea, Schwitzer, Thomas, Aouizerate, Bruno, Llorca, Pierre Michel, Biseul, Isabelle, Etain, B., Moirand, Rémi, Olié, Émilie, Haffen, Emmanuel, Leboyer, Marion, Courtet, Philippe, Icick, Romain, Guillaume, Sébastien, Laboratoire de Psychologie des Pays de la Loire (LPPL), Université d'Angers (UA)-Nantes Université - UFR Lettres et Langages (Nantes Univ - UFR LL), Nantes Université - pôle Humanités, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Humanités, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ), Fondation FondaMental [Créteil], Institut Pascal (IP), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), Nutrition et Neurobiologie intégrée (NutriNeuro), Université de Bordeaux (UB)-Institut Polytechnique de Bordeaux-Ecole nationale supérieure de chimie, biologie et physique-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre Hospitalier Princesse Grace, Institut de Neurosciences de la Timone (INT), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Hôpital Louis Mourier - AP-HP [Colombes], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre de recherche en épidémiologie et santé des populations (CESP), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, [GIN] Grenoble Institut des Neurosciences (GIN), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA), Centre Psychothérapique de Nancy [Laxou] (CPN), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Cohortes épidémiologiques en population (CONSTANCES), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay-Université Paris Cité (UPCité), Centre Hospitalier le Vinatier [Bron], Hôpital Lapeyronie [Montpellier] (CHU), Service de chirurgie pédiatrique [CHU Besançon], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Hôpital Henri Mondor, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Institut de Génomique Fonctionnelle (IGF), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Fondation FondaMental, FFM, Agence Nationale de la Recherche, ANR: ANR-10- COHO-10-01, ANR-11-IDEX-0004-02, Institut National de la Santé et de la Recherche Médicale, Inserm, This research was supported by the FondaMental Foundation , French National Institute of Health and Medical Research (INSERM), Public Hospitals of Paris (AP-HP), and by the French National Research Agency (ANR)'s Investment for the Future program (ref. nos ANR-11-IDEX-0004-02 and ANR-10- COHO-10-01 ). The funding sources had no role in the study design, data collection, analysis, preparation of the manuscript, or decision to submit the manuscript for publication., ANR-11-IDEX-0004,SUPER,Sorbonne Universités à Paris pour l'Enseignement et la Recherche(2011), and ANR-10-COHO-0010,Psy-COH,FondaMental-Cohortes(2010)
Subjects: Psychiatry and Mental health, Clinical Psychology, Bipolar disorder, [SDV.MHEP.PSM]Life Sciences [q-bio]/Human health and pathology/Psychiatrics and mental health, Types, Eating disorders, Comorbidity
Abstract: International audience; Background: Eating disorders (EDs) are liable to alter the disease course of bipolar disorder (BD). We explored the crossed clinical features between EDs and BD, particularly as a function of BD type (BD1 vs. BD2). Methods: 2929 outpatients attending FondaMental Advanced Centers of Expertise were assessed for BD and lifetime EDs with a semi-structured interview, and their sociodemographic, dimensional and clinical data were collected according to a standardized procedure. For each ED type, bivariate analyses were used to investigate associations between these variables and the type of BD type followed by multinomial regressions with the variables associated with EDs and BDs after Bonferroni correction. Results: Comorbid EDs were diagnosed in 478 (16.4 %) cases, and were more prevalent in patients with BD2 than in those with BD1 (20.6 % vs. 12.4 %, p < 0.001). Regression models showed no difference according to the subtype of bipolar disorder on the characteristics of patients with anorexia nervosa (AN), bulimia nervosa (BN) or binge eating disorder (BED). After multiple adjustments, the factors differentiating BD patients with versus without ED were primarily age, gender, body mass index, more affective lability and comorbidity with anxiety disorders. BD patients with BED also scored higher regarding childhood trauma. BD patients with AN also showed higher risk of past suicide attempts than those with BED. Conclusions: In a large sample of patients with BD, we found a high prevalence of lifetime EDs, especially for the BD2 type. EDs were associated with several severity indicators, but not with BD type-specific characteristics. This should prompt clinicians to carefully screen patients with BD for EDs, regardless of BD and ED types.
Published: 2023
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46. Proteomic Investigation of Ram Spermatozoa and the Proteins Conferred by Seminal Plasma

Author: T. Leahy, Simon P. de Graaf, Lucie Combes-Soia, J.P. Rickard, Guillaume Tsikis, Clément Soleilhavoup, Valérie Labas, Grégoire Harichaux, Xavier Druart, Taylor Pini, Faculty of Veterinary Science, School of Life and Environmental Sciences, University of Sydney, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Plateforme d'Analyse Intégrative des Biomolécules, Institut National de la Recherche Agronomique (INRA), NSW Stud Merino Breeders Association Trust and Australian Wool Innovation Limited, The high resolu tion mass spectrometer was financed (SMHART project) by the European Regional Development Fund (ERDF), Conseil Régional du Centre, French National Institute for Agricultural Research (INRA), French National Institute of Health and Medical Research (Inserm), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Male, Proteomics, endocrine system, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], sperm proteome, Seminal Plasma Proteins, Plasma protein binding, Biology, Bioinformatics, Insemination, Biochemistry, Mass Spectrometry, Cell membrane, 03 medical and health sciences, medicine, Animals, ram spermatozoa, reproductive and urinary physiology, Sheep, urogenital system, Cell Membrane, Proteins, General Chemistry, MS, species comparaison, Sperm, Spermatozoa, Metabolic pathway, ovine, 030104 developmental biology, medicine.anatomical_structure, Fertility, Proteome, seminal plasma, Metabolic Networks and Pathways, Chromatography, Liquid, Protein Binding
Abstract: Sperm proteomes have emerged for several species; however, the extent of species similarity is unknown. Sheep are an important agricultural species for which a comprehensive sperm proteome has not been produced. In addition, potential proteomic factors from seminal plasma that may contribute to improved fertility after cervical insemination are yet to be explored. Here we use liquid chromatography-tandem mass spectrometry to investigate the proteome of ejaculated ram spermatozoa, with quantitative comparison to epididymal spermatozoa. We also present a comparison to published proteomes of five other species. We identified 685 proteins in ejaculated ram spermatozoa, with the most abundant proteins involved in metabolic pathways. Only 5% of ram sperm proteins were not detected in other species, which suggest highly conserved structures and pathways. Of the proteins present in both epididymal and ejaculated ram spermatozoa, 7% were more abundant in ejaculated spermatozoa. Only two membrane-bound proteins were detected solely in ejaculated sperm lysates: liver enriched gene 1 (LEG1/C6orf58) and epidermal growth factor-like repeats and discoidin I-like domains 3 (EDIL3). This is the first evidence that despite its relatively complex proteomic composition, seminal plasma exposure leads to few novel proteins binding tightly to the ram sperm plasma membrane.
Published: 2016
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47. Molecular Evolution of the Substrate Specificity of Chloroplastic Aldolases/Rubisco Lysine Methyltransferases in Plants

Author: Océane Gigarel, Sabine Brugière, Jacqueline Martin-Laffon, Marianne Tardif, Morgane Mininno, Stéphane Ravanel, Claude Alban, Sheng Ma, Olivier Bastien, Laboratoire de Physiologie Cellulaire & Végétale [Grenoble] (UGA), Université Grenoble Alpes (UGA), Laboratoire de physiologie cellulaire végétale (LPCV), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie à Grande Échelle (BGE - UMR S1038), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), This work was supported by the Department of Plant Biology and Breeding from the French National Institute for Agricultural Research (AAP BAP2013 INRA_Calvin Cycle-Me) and by the Proteomics French Infrastructure (ANR-10-INBS-08-01)., ANR-10-INBS-08-01/10-INBS-0008,ProFI,Infrastructure Française de Protéomique(2010), Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes (UGA), French National Institute for Agricultural Research (AAP BAP2013 INRA_Calvin Cycle-Me), Physiologie cellulaire et végétale (LPCV), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), ANR-10-INBS-0008,ProFI,Infrastructure Française de Protéomique(2010), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])
Subjects: 0301 basic medicine, Models, Molecular, Methyltransferase, Chloroplasts, Arabidopsis thaliana, Protein subunit, Ribulose-Bisphosphate Carboxylase, [SDV]Life Sciences [q-bio], Protein domain, Amino Acid Motifs, Plant Science, Biology, Substrate Specificity, Evolution, Molecular, 03 medical and health sciences, Protein Domains, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Amino Acid Sequence, SET-domain protein methyltransferase, green lineage, Site-directed mutagenesis, Rosaceae, Molecular Biology, ComputingMilieux_MISCELLANEOUS, Pisum sativum, Aldehyde-Lyases, Genetics, molecular evolution, chimeric enzyme, Chlamydomonas rheinhardtii, RuBisCO, Carbon fixation, fungi, food and beverages, Fabaceae, Plant, Methylation, Chloroplast, Cucurbitaceae, 030104 developmental biology, Biochemistry, biology.protein, Mutagenesis, Site-Directed, methylation, site-directed mutagenesis, Site directed mutagenesis
Abstract: International audience; Rubisco and fructose-1,6-bisphosphate aldolases (FBAs) are involved in CO2 fixation in chloroplasts. Both enzymes are trimethylated at a specific lysine residue by the chloroplastic protein methyltransferase LSMT. Genes coding LSMT are present in all plant genomes but the methylation status of the substrates varies in a species-specific manner. For example, chloroplastic FBAs are naturally trimethylated in both Pisum sativum and Arabidopsis thaliana, whereas the Rubisco large subunit is trimethylated only in the former species. The in vivo methylation status of aldolases and Rubisco matches the catalytic properties of AtLSMT and PsLSMT, which are able to trimethylate FBAs or FBAs and Rubisco, respectively. Here, we created chimera and site-directed mutants of monofunctional AtLSMT and bifunctional PsLSMT to identify the molecular determinants responsible for substrate specificity. Our results indicate that the His-Ala/Pro-Trp triad located in the central part of LSMT enzymes is the key motif to confer the capacity to trimethylate Rubisco. Two of the critical residues are located on a surface loop outside the methyltransferase catalytic site. We observed a strict correlation between the presence of the triad motif and the in vivo methylation status of Rubisco. The distribution of the motif into a phylogenetic tree further suggests that the ancestral function of LSMT was FBA trimethylation. In a recent event during higher plant evolution, this function evolved in ancestors of Fabaceae, Cucurbitaceae, and Rosaceae to include Rubisco as an additional substrate to the archetypal enzyme. Our study provides insight into mechanisms by which SET-domain protein methyltransferases evolve new substrate specificity.
Published: 2016
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48. Intact cell MALDI-TOF MS on sperm: a molecular test for male fertility diagnosis

Author: Elisabeth Blesbois, Laura Soler, Valérie Labas, Aurore Thélie, Isabelle Grasseau, Ana Paula Teixeira-Gomes, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Plateforme d'Analyse Intégrative des Biomolécules, Institut National de la Recherche Agronomique (INRA), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), French National Infrastructure of Research CRB anim, Investissements d'avenir, ANR-11-INBS-0003, French National Institute of Agronomic Research, SMHART project, European Regional Development Fund (ERDF), Conseil Régional du Centre, French National Institute of Health and Medical Research, European Project: 267196, Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), UR Infectiologie animale et Santé publique (UR IASP), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Université de Tours
Subjects: 0301 basic medicine, Male, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], Seminal Plasma Proteins, media_common.quotation_subject, Acrosome reaction, Motility, Fertility, Protein degradation, Biology, Biochemistry, Analytical Chemistry, Andrology, 03 medical and health sciences, Animals, Molecular Biology, media_common, Genetics, 030102 biochemistry & molecular biology, Research, Models, Theoretical, Sperm, Spermatozoa, In vitro, 3. Good health, 030104 developmental biology, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Chickens, Function (biology), Biomarkers
Abstract: Currently, evaluation of sperm quality is primarily based on in-vitro measures of sperm function such as motility, viability and/or acrosome reaction. However, results are often poorly correlated with fertility, and alternative diagnostic tools are therefore needed both in veterinary and human medicine. In a recent pilot study, we demonstrated that MS profiles from intact chicken sperm using MALDI-TOF profiles could detect significant differences between fertile/subfertile spermatozoa showing that such profiles could be useful for in-vitro male fertility testing. In the present study, we performed larger standardized experimental procedures designed for the development of fertility- predictive mathematical models based on sperm cell MALDI-TOF MS profiles acquired through a fast, automated method. This intact cell MALDI-TOF MS-based method showed high diagnostic accuracy in identifying fertile/subfertile males in a large male population of known fertility from two distinct genetic lineages (meat and egg laying lines). We additionally identified 40 % of the m/z peaks observed in sperm MS profiles through a top-down high-resolution protein identification analysis. This revealed that the MALDI-TOF MS spectra obtained from intact sperm cells contained a large proportion of protein degradation products, many implicated in important functional pathways in sperm such as energy metabolism, structure and movement. Proteins identified by our predictive model included diverse and important functional classes providing new insights into sperm function as it relates to fertility differences in this experimental system. Thus, in addition to the chicken model system developed here, with the use of appropriate models these methods should effectively translate to other animal taxa where similar tests for fertility are warranted.
Published: 2016
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49. Impact of Different Front-of-Pack Nutrition Labels on Consumer Purchasing Intentions A Randomized Controlled Trial

Author: Ducrot, Pauline, Julia, Chantal, Mejean, Caroline, Kesse-Guyot, Emmanuelle, Touvier, Mathilde, Fezeu Kamedjie, Léopold, Hercberg, Serge, Péneau, Sandrine, Centre de Recherche Épidémiologie et Statistique Sorbonne Paris Cité (CRESS (U1153 / UMR_A_1125 / UMR_S_1153)), Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA), French Ministry of Health (DGS), National Institute for Prevention and Health Education (INPES), French Institute for Public Health Surveillance, French National Institute for Health and Medical Research (INSERM), French National Institute for Agricultural Research, National Conservatory for Arts and Crafts, INPES, and University of Paris 13
Subjects: Adult, Male, Adolescent, [SDV]Life Sciences [q-bio], Health Behavior, digestive, oral, and skin physiology, food choice, health, Health Promotion, Consumer Behavior, Middle Aged, Choice Behavior, information, Food Preferences, Young Adult, Food Labeling, Humans, Female, Nutritive Value, nutrient profiling system, Aged
Abstract: International audience; Introduction: Despite growing evidence supporting the utility of front-of-pack nutrition labels in enabling consumer evaluation of food product healthiness, research on food choices is scarce. This study aims at comparing the impact of front-of-pack nutrition labels on consumers' purchasing intentions. Design: Five-arm, open-label RCT. Setting/participants: The study setting was a virtual web-based supermarket, with participants from the French NutriNet-Sante study. The eligibility requirement was grocery shopping involvement. Intervention: The intervention was to simulate one shopping situation with front-of-pack nutrition labels affixed on food products (December 2014 to March 2015). Participants were randomly assigned to one of five exposure conditions using a central computer system: Guideline Daily Amounts, Multiple Traffic Lights, Five-Color Nutrition Label, Green Tick, or control (no front-of-pack exposure). Given the nature of the intervention, masking of participants was not performed. Main outcome measures: The primary outcome was the overall nutritional quality of the contents of the shopping cart, estimated using the United Kingdom Food Standards Agency nutrient profiling system. Secondary outcomes included energy and nutrient content of the shopping cart. Impact of the front-of-pack labels was also evaluated across sociodemographic subgroups based on age, educational level, income, and nutrition knowledge. Results: A total of 11,981 participants were included in the analyses (April 2015). The Five-Color Nutrition Label significantly led to the highest overall nutritional quality of the shopping cart, as reflected by lower Food Standards Agency scores(M=8.72; SD=2.75), followed by Multiple Traffic Lights (M=8.97; SD=2.68) and Green Tick (M=8.99; SD=2.71), compared with the control (M=9.34; SD=2.57) (p
Published: 2016
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50. Proteomes of the female genital tract during the oestrous cycle

Author: Philippa L. Kohnke, Simon P. de Graaf, Karine Reynaud, Grégoire Harichaux, Guillaume Tsikis, Xavier Druart, Cindy Riou, Valérie Labas, Clément Soleilhavoup, Nadine Gérard, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Plate-forme d'Analyse Intégrative des Biomolécules, Institut National de la Recherche Agronomique (INRA), École nationale vétérinaire d'Alfort (ENVA), Faculty of Veterinary Science, University of Sydney, French Agence Nationale de la Recherche, Australian Wool Innovation and the NSW Stud Merino Breeders Association Trust, (SMHART project) by the European Regional Development Fund (ERDF), Conseil Régional du Centre, French National Institute for Agricultural Research (INRA), French National Institute of Health and Medical Research (Inserm), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Proteomics, medicine.medical_specialty, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], Proteome, Blotting, Western, Uterus, Cervix Uteri, Oviducts, Luteal phase, Biology, Biochemistry, Analytical Chemistry, Andrology, 03 medical and health sciences, Estrus, Tandem Mass Spectrometry, Internal medicine, Heat shock protein, medicine, Animals, HSPA8, Molecular Biology, Fertilisation, Sheep, urogenital system, Research, Mucin, Genitalia, Female, Immunohistochemistry, 030104 developmental biology, Endocrinology, medicine.anatomical_structure, Oviduct, Female, Chromatography, Liquid
Abstract: The female genital tract includes several anatomical regions whose luminal fluids successively interact with gametes and embryos and are involved in the fertilisation and development processes. The luminal fluids from the inner cervix, the uterus and the oviduct were collected along the oestrous cycle at oestrus (Day 0 of the cycle) and during the luteal phase (Day 10) from adult cyclic ewes. The proteomes were assessed by GeLC-MS/MS and quantified by spectral counting. A set of 940 proteins were identified including 291 proteins differentially present along the cycle in one or several regions. The global analysis of the fluid proteomes revealed a general pattern of endocrine regulation of the tract, with the cervix and the oviduct showing an increased differential proteins abundance mainly at oestrus while the uterus showed an increased abundance mainly during the luteal phase. The proteins more abundant at oestrus included several families such as the heat shock proteins (HSP), the mucins, the complement cascade proteins and several redox enzymes. Other proteins known for their interaction with gametes such as oviductin (OVGP), osteopontin, HSPA8, and the spermadhesin AWN were also overexpressed at oestrus. The proteins more abundant during the luteal phase were associated with the immune system such as ceruloplasmin, lactoferrin, DMBT1, or PIGR, and also with tissue remodeling such as galectin 3 binding protein, alkaline phosphatase, CD9, or fibulin. Several proteins differentially abundant between estrus and the luteal phase, such as myosin 9 and fibronectin, were also validated by immunohistochemistry. The potential roles in sperm transit and uterine receptivity of the proteins differentially regulated along the cycle in the female genital tract are discussed.
Published: 2016
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