16 results on '"Franco-Topete R"'
Search Results
2. Obesity and diabetes as a risk factor of chronic disease of the liver in the regional hospital
- Author
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Rodríguez-Villa, P., primary, Brizuela-Santana, J., additional, Jiménez-Partida, A., additional, Jiménez-Partida, M., additional, Ramírez-Flores, S., additional, Cortés-Aguilar, Y., additional, Bravo-Cuellar, A., additional, Franco-Topete, R., additional, and Sepúlveda-Castro Rogelio, R., additional
- Published
- 2020
- Full Text
- View/download PDF
3. Inmmunophenotypic classification of medulloblastoma and its correlation with clinical and histopathological variables of prognostic value.
- Author
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Santana Bejarano, M. B., Ortuño Sahagún, D., Franco Topete, R., López Venegas, S., Nava Villalba, M., Puebla Mora, A., and Godínez Rubí, J. M.
- Subjects
MEDULLOBLASTOMA ,TUMOR diagnosis ,HISTOPATHOLOGY - Abstract
Medulloblastomas (MB) are aggressive neoplasms of the central nervous system of embryonic origin. In 2016, the WHO proposed a new categorization that complements the previous histopathological classification and divides them into four molecular subgroups: WNT activated, SHH activated, group 3 and 4. Due to the complexity and cost of molecular methods, alternative methodologies have been sought to approach molecular diagnosis in a more affordable way. A panel of biomarkers identifiable by immunohistochemistry (IHC) has been proposed that would allow inferring the molecular group and thus predict the behavior of the neoplasm. The objective wasTo classify cases of MB according to the immunophenotype proposed for each molecular group, and to determine its association with clinical and histopathological variables with prognostic value. Observational, retrospective cohort follow-up study. Cases of patients with diagnosis of MB were selected in a period of 10 years from a public hospital. Relevant clinical and histopathological data were recorded. A IHC panel of six biomarkers was performed: ßcatenin, GAB1, YAP1, p75NTR, p53 and Ki- 67. Twenty-six cases of MB were included for analysis: 1 case (3.8%) WNT, 15 cases (57.7%) SHH, 6 cases (23.1%) No WNT/SHH, 4 cases (15.4%) NOS. A significant association was found between p53 expression and the variables recurrence/progression (p=0.014) and disease-free survival (p=0.008). The remaining variables did not show significant differences between the groups. No association was found between immunophenotype classification and the other variables. The percentage of p53 expression is associated with events related to poor prognosis (recurrence/progression and shorter disease-free survival time). [ABSTRACT FROM AUTHOR]
- Published
- 2019
4. National consensus of diagnosis and treatment of non-small cell lung cancer,Consenso nacional de diagnóstico y tratamiento del cáncer de pulmón de células no pequeñas
- Author
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Arrieta, O., Guzmán-De Alba, E., Alba-López, L. F., Acosta-Espinoza, A., Alatorre-Alexander, J., Alexander-Meza, J. F., Allende-Pérez, S. R., Alvarado-Aguilar, S., Araujo-Navarrete, M. E., Argote-Greene, L. M., Aquino-Mendoza, C. A., Astorga-Ramos, A. M., Astudillo-De La Vega, H., Avilés-Salas, A., Barajas-Figueroa, L. J., Barroso-Quiroga, N., Blake-Cerda, M., Cabrera-Galeana, P. A., Calderillo-Ruíz, G., Campos-Parra, A. D., Cano-Valdez, A. M., Capdeville-García, D., Castillo-Ortega, G., Casillas-Suárez, C., Castillo-González, P., Corona-Cruz, J. F., Correa-Acevedo, M. E., Cortez-Ramírez, S. S., Jhony A. De La Cruz-Vargas, La Garza-Salazar, J. G., La Mata-Moya, M. D., La Peña-Hinojosa, C., Domínguez-Flores, M. E., Domínguez-Malagón, H. R., Domínguez-Parra, L. M., Domínguez-Peregrina, A., Durán-Alcocer, J., Enríquez-Aceves, M. I., Elizondo-Ríos, A., Escobedo-Sánchez, M. D., Villafranca, P. E. -M, Flores-Cantisani, A., Flores-Gutiérrez, J. P., Franco-Marina, F., Franco-González, E. E., Franco-Topete, R. A., Fuentes-De La Peña, H., Galicia-Amor, S., Gallardo-Rincón, D., Gamboa-Domínguez, A., García-Andreu, J., García-Cuéllar, C. M., García-Sancho-Figueroa, M. C., García-Torrentera, R., Gerson-Cwilich, R., Gómez-González, A., Green-Schneeweiss, L., Del Rocío Guillén-Núñez, M., Gutiérrez-Velázquez, H., Ibarra-Pérez, C., Jiménez-Fuentes, E., Juárez-Sánchez, P., Juárez-Ramiro, A., Kelly-García, J., Kuri-Exsome, R., Lázaro-León, J. M., León-Rodríguez, E., Llanos-Osuna, S., Loyola-García, U., López-González, J. S., López Yde Antuñano, F. J., Loustaunau-Andrade, M. A., Macedo-Pérez, E. O., Machado-Villarroel, L., Magallanes-Maciel, M., Martínez-Barrera, L., Martínez-Cedillo, J., Martínez-Martínez, G., Medina-Esparza, A., Meneses-García, A., Mohar-Betancourt, A., Blanhir, J. M., Morales-Gómez, J., Motola-Kuba, D., Nájera-Cruz, M. P., Del Carmen Núñez-Valencia, C., Ocampo-Ocampo, M. A., Ochoa-Vázquez, M. D., Olivares-Torres, C. A., Palomar-Lever, A., Patiño-Zarco, M., Pérez-Padilla, R., Peña-Alonso, Y. R., Pérez-Romo, A. R., Pérez, M. A., Pinaya-Ruíz, P. M., Pointevin-Chacón, M. A., Poot-Braga, J. J., Posadas-Valay, R., Ramírez-Márquez, M., Reyes-Martínez, I., Robledo-Pascual, J., Rodríguez-Cid, J., Rojas-Marín, C. E., Romero-Bielma, E., Rubio-Gutiérrez, J. E., Sáenz-Frías, J. A., Salazar-Lezama, M. Á, Sánchez-Lara, K., Martínez, R. S., Santillán-Doherty, P., Alejandro-Silva, J., Téllez-Becerra, J. L., Toledo-Buenrostro, V., Torre-Bouscoulet, L., Torecillas-Torres, L., Torres, M., Tovar-Guzmán, V., Turcott-Chaparro, J. G., Vázquez-Cortés, J. J., Vázquez-Manríquez, M. E., Vilches-Cisneros, N., Villegas-Elizondo, J. F., Zamboni, M. M., Zamora-Moreno, J., and Zinser-Sierra, J. W.
5. Implementing Standard Diagnosis and Treatment for Locally Advanced Breast Cancer Through Global Research in Latin America: Results From a Multicountry Pragmatic Trial.
- Author
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Retamales J, Daneri-Navarro A, Artagaveytia N, Alves da Quinta D, Abdelhay E, Podhajcer OL, Velázquez C, Giunta D, Crocamo S, Garibay-Escobar A, Del Toro-Arreola A, Rodriguez R, Aghazarian M, Alcoba E, Alonso I, Binato R, Bravo AI, Canton-Romero J, Carraro DM, Castro M, Castro-Cervantes J, Cataldi S, Camejo N, Cortes-Sanabria L, Flores-Marquez M, Laviña G, Musetti E, Caserta B, Cerda M, Colombo A, Delgadillo-Cristerna R, Dreyer Breitenbach M, Fernandez E, Fernandez J, Franco-Topete R, Gabay C, Gaete F, Gamboa J, García-Gaeta R, Gomez Del Toro M, Gonzalez-Ramirez LP, Guerrero M, Herrera-Miramontes M, Lopez-Vasquez A, Maldonado S, Morán-Mendoza A, Morgan-Villela G, Nagai MA, Navarro-Ruiz N, Oceguera-Villanueva A, Ortiz MA, Quintero J, Quintero-Ramos A, Ramirez-Rosales G, Ramos-Ramirez M, Chiquitelli Marques MM, Rivera Claisse E, Rodriguez-Gonzalez D, Romero-Gomez A, Rosales C, Salas-Gonzalez E, Sanchotena V, Segovia L, Silva-García AA, Valenzuela-Antelo O, Venegas-Godinez L, Zagame L, Gomez J, Llera AS, and Müller B
- Subjects
- Humans, Female, Middle Aged, Latin America epidemiology, Adult, Aged, Breast Neoplasms therapy, Breast Neoplasms diagnosis, Breast Neoplasms pathology, Breast Neoplasms mortality, Neoadjuvant Therapy
- Abstract
Purpose: Breast cancer mortality rates in Latin America (LA) are higher than those in the United States, possibly because of advanced disease presentation, health care disparities, or unfavorable molecular subtypes. The Latin American Cancer Research Network was established to address these challenges and to promote collaborative clinical research. The Molecular Profiling of Breast Cancer Study (MPBCS) aimed to evaluate the clinical characteristics and treatment outcomes of LA participants with locally advanced breast cancer (LABC)., Patients and Methods: The MPBCS enrolled 1,449 participants from Argentina, Brazil, Chile, Mexico, and Uruguay. Through harmonized procedures and quality assurance measures, this study evaluated clinicopathologic characteristics, neoadjuvant chemotherapy response, and survival outcomes according to residual cancer burden (RCB) and the type of surgery., Results: Overall, 711 and 480 participants in the primary surgery and neoadjuvant arms, respectively, completed the 5-year follow-up period. Overall survival was independently associated with RCB (worse survival for RCBIII-adjusted hazard ratio, 8.19, P < .001, and RCBII [adjusted hazard ratio, 3.69, P < .008] compared with RCB0 [pathologic complete response or pCR]) and type of surgery (worse survival in mastectomy than in breast-conserving surgery [BCS], adjusted hazard ratio, 2.97, P = .001). The hormone receptor-negative-human epidermal growth factor receptor 2-positive group had the highest proportion of pCR (48.9%). The analysis of the ASCO Quality Oncology Practice Initiative breast module revealed high compliance with pathologic standards but lower adherence to treatment administration standards. Notably, compliance with trastuzumab administration varied widely among countries (33.3%-88.7%)., Conclusion: In LABC, we demonstrated the survival benefit of BCS and the prognostic effect of the response to available neoadjuvant treatments despite an important variability in access to key treatments. The MPBCS represents a significant step forward in understanding the real-world implementation of oncologic procedures in LA.
- Published
- 2024
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6. Kinematic Locomotion Changes in C57BL/6 Mice Infected with Toxoplasma Strain ME49.
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Galván-Ramírez ML, Salas-Lais AG, Dueñas-Jiménez SH, Mendizabal-Ruiz G, Franco Topete R, Berumen-Solís SC, Rodríguez Pérez LR, and Franco Topete K
- Abstract
Chronic infection with the intracellular parasite Toxoplasma gondii produces an accumulation of cysts in the brain and muscle, causing tissue damage. The cysts in the brain motor regions affect some kinematic locomotion parameters in the host. To localize the brain cysts from Toxoplasma gondii and study the changes in kinematic locomotion in C57BL/6 mice. Female adult C57BL/6 mice were infected orally with 30 ME-49 Toxoplasma gondii cysts. An uninfected group ( n = 7) and two infected groups, examined 15 and 40 days postinfection, were used for this study. To evaluate kinematic locomotion, the mice were marked with indelible ink on the iliac crest, hip, knee, ankle, and phalangeal metatarsus of the left and right hindlimbs. At least three recordings were carried out to obtain videos of the left and right hindlimbs. Mice were video recorded at 90 fps at a resolution of 640 × 480 pixels while walking freely in a transparent Plexiglass tunnel. We measured the hindlimb pendular movement and the hindlimb transfer [linear displacement] curves for each step and evaluated them statistically with Fréchet dissimilarity tests. Afterward, the mice were sacrificed, and the brain, heart, skeletal muscle, lung, liver, and kidney were obtained. The different tissues were stained with hematoxylin and eosin for analysis with optical microscopy. Topographic localization of the cysts was made using bregma coordinates for the mouse brain. The cysts were distributed in several brain regions. In one mouse, cyst accumulation occurred in the hippocampus, coinciding with an alteration in foot displacement. The step length was different among the different studied groups.
- Published
- 2019
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7. IFN-γR2 is strongly expressed on endothelial cells of gingival tissues from patients with chronic periodontitis.
- Author
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Franco-Topete R, Zepeda-Nuño JS, Zamora-Perez AL, Fuentes-Lerma MG, Gómez-Meda BC, and Guerrero-Velázquez C
- Subjects
- Adult, Biopsy, Case-Control Studies, Enzyme-Linked Immunosorbent Assay, Female, Humans, Immunohistochemistry, Male, Middle Aged, Reference Values, Statistics, Nonparametric, Interferon gamma Receptor, Chronic Periodontitis pathology, Endothelial Cells pathology, Gingiva pathology, Interferon-gamma analysis, Receptors, Interferon analysis
- Abstract
Objective: Chronic periodontitis (CP) is characterized by gingival inflammation and bone destruction. It has been reported that interferon-gamma (IFN-γ) levels are high in CP patients; however, the IFN-γ receptor (IFN-γR) has not been studied in gingival tissue from these patients. To evaluate IFN-γ levels and IFN-γR expression in gingival tissue biopsies from chronic periodontitis patients compared with healthy subjects (HS)., Material and Methods: Gingival tissues were obtained from all study subjects, CP (n = 18) and healthy subjects (HS) (n = 12). A tissue section of each study subject was embedded in paraffin blocks to determine the expression of IFN-γ R (IFN-γR1 and IFN-γR2) through immunohistochemistry. Another section of the tissue was homogenized and IFN-γ was measured by the ELISA technique., Results: No significant differences were found in the IFN-γR1 expression within the cell layers of the gingival tissue of the study groups. When analyzing the IFN-γR2 expression it was found that IFN-γR2 is strongly expressed in the endothelial cells of CP patients when compared to HS (p<0.05). IFN-γ concentrations in the gingival tissue were significantly higher in CP patients than in HS. No significant correlation between IFN-γ levels and the expression of IFN-γR1 and IFN-γR2 was found. However, a positive correlation between IFN-γ levels and clinical parameters [probing depth (PD) and clinical attachment level (CAL)] was found., Conclusion: The study of IFN-γR expression in gingival tissue samples from patients with CP showed an increase only in the IFN-γR2 chain in endothelial cells when compared to HS.
- Published
- 2018
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8. Expression profile of NF-κB regulated genes in sporadic colorectal cancer patients.
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González-Quezada BA, Santana-Bejarano UF, Corona-Rivera A, Pimentel-Gutiérrez HJ, Silva-Cruz R, Ortega-De-la-Torre C, Franco-Topete R, Franco-Topete K, Centeno-Flores MW, Maciel-Gutiérrez VM, Corona-Rivera JR, Armendáriz-Borunda J, and Bobadilla-Morales L
- Abstract
Colorectal cancer (CRC) is the fourth leading worldwide cause of cancer-associated mortalities. Nuclear factor-κB (NF-κB) is a transcriptional regulator of multiple genes associated with CRC. Tumor tissue were compared with normal adjacent mucosa from 30 sporadic patients with CRC were investigated. A total of 8 non-CRC patients were analyzed as a control group. In the present study, the protein expression of NF-κB/p65 was detected by immunohistochemistry, and the gene expression profiles of cyclin D1 (CCND1) , prostaglandin-endoperoxide synthase 2, vascular endothelial growth factor A, matrix metallopeptidase 9, BCL2 apoptosis regulator (BCL2) , BCL2 like 1, nitric oxide synthase 2, tumor necrosis factor and arachidonate lipoxygenase were detected by reverse transcription-quantitative polymerase chain reaction. NF-κB/p65 and genes expression profiles were classified according to tumor-node-metastasis (TNM) clinicopathological parameters, followed by statistical analysis. Higher protein expression of NF-κB/p65 in the cytoplasm of tumor tissues compared with adjacent normal mucosa was reported; this increment was positively associated with all clinicopathological parameters, except for tumor localization site. The selected genes demonstrated a diverse associative pattern when analyzed with clinicopathological parameters. CCND1 was positively associated with all TNM parameters and BCL2 was negatively associated with all TNM parameters, thus indicating their importance as strong molecular biomarkers for CRC. According to these results, not all selected genes regulated by NF-κB/p65 show increased expression during CRC development, whereas the transcription factor did. The present study suggests that NF-κB/p65 overexpression is necessary for CRC establishment and progression, but its transcriptional activity is not sufficient to regulate all target genes in CRC. NF-κB/p65 and the gene expression profiles reported in the present study may be therapeutically useful. Considering the heterogeneity of the disease, the particular evaluation of these molecules may allow for the selection of proper diagnosis, treatment and follow-up for patients with sporadic CRC.
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- 2018
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9. Chronic exposure to endosulfan induces inflammation in murine colon via β-catenin expression and IL-6 production.
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Téllez-Bañuelos MC, Haramati J, Franco-Topete K, Peregrina-Sandoval J, Franco-Topete R, and Zaitseva GP
- Subjects
- 1,2-Dimethylhydrazine administration & dosage, Administration, Oral, Animals, Colorectal Neoplasms immunology, Endosulfan immunology, Female, Humans, Inflammation Mediators metabolism, Interleukin-6 metabolism, Mice, Mice, Inbred C57BL, P-Selectin metabolism, Pesticides immunology, Tumor Necrosis Factor-alpha metabolism, beta Catenin genetics, beta Catenin metabolism, Colitis immunology, Colon immunology, Colorectal Neoplasms epidemiology, Endosulfan administration & dosage, Inflammation immunology
- Abstract
Endosulfan (ENDO) is a widely used organochlorine (OC) pesticide and persistent organo-pollutant. Epidemiological studies have shown that high levels of OC exposure were related to colorectal cancer (CRC) incidence. The objectives of the present study were to evaluate histological changes in the colon, as well as in in situ expression of β-catenin and P-selectin, and serum levels of select pro-inflammatory cytokines in mice administered ENDO; there is a relationship between increased serum IL-6 and P-selectin levels in CRC patients and aberrant β-catenin signaling is important in initiation/maintenance of most CRCs. Mice were exposed to ENDO (at dose < LD
50 ) orally once a week for up to 24 weeks, and monitored (inclusive) for a total of 42 weeks. The experiment was comprised of three groups, one that did not receive ENDO (olive oil vehicle), one administered 2 mg ENDO/kg/week and a positive control (for induction of CRC) given a weekly 20 mg 1,2-dimethylhydrazine (DMH)/kg injection. The results indicated that oral administration of ENDO provoked moderate inflammation starting at six weeks, and severe colonic inflammation with an appearance of dysplastic formations (aberrant crypts) in mice treated with ENDO (or DMH) for 12 weeks or longer. Serum IL-6 levels significantly increased starting at six weeks and rose to a peak of 15-fold higher than in controls at 42 weeks; TNFα levels likewise significantly increased, with a later peak (≈four-fold higher than controls) at 30-42 weeks. Immunohistochemical analysis of the colon also showed that expression of β-catenin and P-selectin increased with length of exposure to ENDO. Taken together, the results indicate that continued repeated oral exposure to ENDO induces increased expression of β-catenin and P-selectin, inflammation in the colon, and, ultimately, local tissue dysplasia.- Published
- 2016
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10. Prolactin and prolactin receptor expression in cervical intraepithelial neoplasia and cancer.
- Author
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Ascencio-Cedillo R, López-Pulido EI, Muñoz-Valle JF, Villegas-Sepúlveda N, Del Toro-Arreola S, Estrada-Chávez C, Daneri-Navarro A, Franco-Topete R, Pérez-Montiel D, García-Carrancá A, and Pereira-Suárez AL
- Subjects
- Biopsy, Cell Line, Tumor, Cervix Uteri metabolism, Cervix Uteri pathology, Female, Humans, Immunohistochemistry, Neoplasm Grading, Prolactin genetics, Receptors, Prolactin genetics, Signal Transduction physiology, Uterine Cervical Neoplasms pathology, Uterine Cervical Neoplasms physiopathology, Uterine Cervical Dysplasia pathology, Uterine Cervical Dysplasia physiopathology, Gene Expression Regulation, Neoplastic physiology, Prolactin metabolism, Receptors, Prolactin metabolism, Uterine Cervical Neoplasms metabolism, Uterine Cervical Dysplasia metabolism
- Abstract
Prolactin receptor (PRLR) overexpression could play a role in tumorigenesis. The aim of this study was to determine prolactin (PRL) and PRLR expression in biopsies from patients with precursor lesions and uterine cervical cancer. PRLR expression was analyzed in 63 paraffin-embedded biopsies of uterine cervical tissue. In total, eleven low-grade squamous intraepithelial lesions (LSIL), 23 high-grade squamous intraepithelial lesions (HSIL), 21 uterine cervical cancers (UCC) and 8 normal epithelium (NE) were examined using immunoperoxidase staining and Western blot analysis. Additionally, PRL expression was identified in human cervical cancer serum and tissues. The PRLR expression was found to be significantly increased in cervical cancer in comparison with normal tissue and precursor lesions (P < 0.0003). The presence of the long isoform of the PRLR was observed only in cervical cancer tissues. Serum PRL levels were normal in all samples and local prolactin expression was similar in precursor lesions and cervical cancer by Western blot analysis. Our data suggest a possible role for PRLR in the progression of cervical cancer.
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- 2015
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11. 1236 C/T and 3435 C/T polymorphisms of the ABCB1 gene in Mexican breast cancer patients.
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Gutierrez-Rubio SA, Quintero-Ramos A, Durán-Cárdenas A, Franco-Topete RA, Castro-Cervantes JM, Oceguera-Villanueva A, Jiménez-Pérez LM, Balderas-Peña LM, Morgan-Villela G, Del-Toro-Arreola A, and Daneri-Navarro A
- Subjects
- ATP Binding Cassette Transporter, Subfamily B genetics, Adult, Aged, Aged, 80 and over, Alleles, Case-Control Studies, Ethnicity, Female, Genetic Predisposition to Disease, Genotype, Humans, Mexico, Middle Aged, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Postmenopause, Premenopause, Risk Factors, Breast Neoplasms ethnology, Breast Neoplasms genetics, Gene Expression Regulation, Neoplastic, Polymorphism, Single Nucleotide
- Abstract
MDR1, which is encoded by the ABCB1 gene, is involved in multidrug resistance (hydrophobic), as well as the elimination of xenotoxic agents. The association between ABCB1 gene polymorphisms and breast cancer risk in different populations has been described previously; however, the results have been inconclusive. In this study, we examined the association between polymorphisms 3435 C/T and 1236 C/T in the ABCB1 gene and breast cancer development in Mexican women according to their menopausal status and molecular classification. Molecular subtypes as well as allele and genotype frequencies were analyzed. A total of 248 women with initial breast cancer diagnosis and 180 ethnically matched, healthy, unrelated individuals were enrolled. Polymerase chain reaction-restriction fragment length polymorphism was performed to detect polymorphisms 3435 C/T and 1236 C/T in the ABCB1 gene. Premenopausal T allele carriers of the 3435 C/T polymorphism showed a 2-fold increased risk of breast cancer with respect to the reference and postmenopausal groups, as well as triple-negative expression regarding the luminal A/B molecular subrogated subtypes. In contrast, the CT genotype of the 1236 polymorphism was a protective factor against breast cancer. We conclude that the T allele carrier of the 3435 C/T polymorphism in the ABCB1 gene in combination with an estrogen receptor-negative status may be an important risk factor for breast cancer development in premenopausal women.
- Published
- 2015
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12. Association between polymorphisms in the thymidylate synthase gene and risk of breast cancer in a Mexican population.
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Quintero-Ramos A, Gutiérrez-Rubio SA, Del Toro-Arreola A, Franco-Topete RA, Oceguera-Villanueva A, Jiménez-Pérez LM, Castro-Cervantes JM, Barragán-Ruiz A, Vázquez-Camacho JG, and Daneri-Navarro A
- Subjects
- 3' Untranslated Regions genetics, 5' Untranslated Regions genetics, Adult, Alleles, Female, Gene Frequency, Genotype, Haplotypes, Humans, INDEL Mutation, Mexico, Middle Aged, Polymorphism, Single Nucleotide, Postmenopause genetics, Premenopause genetics, Risk Factors, Tandem Repeat Sequences genetics, Young Adult, Breast Neoplasms genetics, Genetic Predisposition to Disease genetics, Polymorphism, Genetic, Thymidylate Synthase genetics
- Abstract
Breast cancer (BC) is the leading cause of cancer-related deaths among women in Mexico. Two single-nucleotide polymorphisms (SNPs) in the thymidylate synthase (TS) gene, the 28-base pair (bp) tandem repeat in the TS 5'-untranslated enhanced region (TSER) and the 6-bp insertion/deletion in the TS 3'-untranslated region (TS 3'-UTR), increase the rate of misincorporation of uridylate into DNA and may lead to chromosomal damage. We examined the association between these polymorphisms and BC risk in Mexican women according to menopause status. Mexican patients with initial BC diagnosis (N = 230) and 145 individuals from a reference general population group (RGP) were included. For statistical analysis, the BC group was divided into pre- and post-menopause groups (PRE and POST groups, respectively). We analyzed both TS polymorphisms (TSER and TS 3'-UTR) using polymerase chain reaction. Finetti analysis was used to evaluate inter-and intra-group differences. The results showed a high frequency for the 3R and ins6 alleles in the BC, RGP, PRE, and POST groups. No significant differences were observed for the TS and TSER genotype and allele frequency distributions between groups. We found that the TSER and TS 3'-UTR SNPs are not associated with BC risk in Mexican patients.
- Published
- 2014
- Full Text
- View/download PDF
13. Protease-activated receptor-2 (PAR-2) in cervical cancer proliferation.
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Sánchez-Hernández PE, Ramirez-Dueñas MG, Albarran-Somoza B, García-Iglesias T, del Toro-Arreola A, Franco-Topete R, and Daneri-Navarro A
- Subjects
- Alphapapillomavirus genetics, Carcinoma, Squamous Cell pathology, Carcinoma, Squamous Cell virology, Cell Growth Processes drug effects, Cell Growth Processes physiology, Cell Line, Tumor, Female, Flow Cytometry, HeLa Cells, Humans, Immunohistochemistry, Neoplasm Staging, Papillomavirus Infections metabolism, Papillomavirus Infections pathology, Paraffin Embedding, Polymerase Chain Reaction, Trypsin biosynthesis, Trypsin pharmacology, Uterine Cervical Neoplasms pathology, Uterine Cervical Neoplasms virology, Carcinoma, Squamous Cell metabolism, Receptor, PAR-2 biosynthesis, Uterine Cervical Neoplasms metabolism
- Abstract
Objective: Protease-activated receptor-2 (PAR-2) is a G-protein-coupled receptor that is cleaved and activated by trypsin and tryptase. There is evidence that PAR-2 contributes to tumor progression in stomach, colon, pancreas, prostate and breast cancer patients. However, the role of PAR-2 in cervical cancer is still unknown. The aim of this work was to study the PAR-2 expression in cervical cancer tissues and the effect of PAR-2 activation on cervical cancer proliferation., Methods: Immunohistochemistry was used to analyze PAR-2 expression in fixed paraffin-embedded tumor tissue from 16 patients with invasive cervical cancer. HPV types were identified by PCR. PAR-2 expression in UISO-SQC-1, HeLa, SiHa, CasKi and C-33 A cervical cancer cell lines was evaluated by flow cytometry. Trypsin was detected by Western blot. Tumor proliferation in response to trypsin or agonist peptide was evaluated by the MTT method., Results: A strong correlation between trypsin and PAR-2 expression in five cervical cancer cell lines, in association with proliferative growth in the presence of trypsin or agonist peptide, was found. All tumors from cervical cancer patients expressed PAR-2 (immunoreactive score was higher in poorly differentiated tumors)., Conclusions: Results suggest that trypsin and PAR-2 are involved in cervical cancer cell proliferation.
- Published
- 2008
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14. CEACAM1 in cervical cancer and precursor lesions: association with human papillomavirus infection.
- Author
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Albarran-Somoza B, Franco-Topete R, Delgado-Rizo V, Cerda-Camacho F, Acosta-Jimenez L, Lopez-Botet M, and Daneri-Navarro A
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- Adult, Antigens, CD analysis, Cell Adhesion Molecules analysis, Disease Progression, Female, Humans, Immunohistochemistry, Middle Aged, Papillomavirus Infections complications, Polymerase Chain Reaction, Precancerous Conditions chemistry, Precancerous Conditions virology, Sensitivity and Specificity, Uterine Cervical Neoplasms chemistry, Uterine Cervical Neoplasms virology, Uterine Cervical Dysplasia chemistry, Uterine Cervical Dysplasia virology, Antigens, CD biosynthesis, Cell Adhesion Molecules biosynthesis, Papillomavirus Infections pathology, Precancerous Conditions pathology, Uterine Cervical Neoplasms pathology, Uterine Cervical Dysplasia pathology
- Abstract
Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is an adhesion molecule expressed in a wide variety of tissues including epithelial cells, leukocytes, and tumors that may establish both homotypic and heterotypic interactions. The aim of this work was to study the protein expression pattern of CEACAM1 in cervical cancer and precursor lesions in the context of human papillomavirus (HPV) infection. We used immunohistochemistry to analyze CEACAM1 expression in formalin-fixed, paraffin-embedded cervical tissues from 15 healthy women, 15 patients with low-grade squamous intraepithelial lesions (SIL), 15 patients with high-grade SIL, and 15 patients with squamous carcinomas. HPV types were identified by PCR. CEACAM1 was either undetectable (13/15) or low (2/15) in normal cervical tissues. By contrast, CEACAM1 expression was increased in high-grade SIL (10 samples staining intermediate/high and 4 samples staining low) as compared with low-grade SIL with undetectable (n=3) or low (n=12) expression. CEACAM1 expression was undetectable or low in cervical carcinoma. Our results suggest that CEACAM1 may be an interesting progression marker in SIL and cervical cancer, in particular due to reported immunoregulatory properties.
- Published
- 2006
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15. Atypical parasitic ischiopagus conjoined twins.
- Author
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Corona-Rivera JR, Corona-Rivera E, Franco-Topete R, Acosta-León J, Aguila-Dueñas V, and Corona-Rivera A
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- Fatal Outcome, Humans, Infant, Newborn, Lumbar Vertebrae diagnostic imaging, Male, Pelvis abnormalities, Radiography, Leg abnormalities, Lumbar Vertebrae abnormalities, Twins, Conjoined pathology
- Abstract
Occurrence of asymmetrical or parasitic conjoined twins (CT) is rare, and currently they are classified analogically to the common unions of symmetrical CT. The authors report on an infant with a parasitic third limb attached to the left lateral aspect of the autosite trunk, in whom male gonadal tissue was found histologically. Parasite parts included complete left lower limb, hemipelvis, lumbosacral vertebral column, spinal cord, and one kidney with ureter and adrenal gland. Autosite anomalies comprised a small left diaphragmatic defect, omphalocele, exstrophy of cloaca, and lumbar meningomyelocele. The authors considered this case to be a rare atypical parasitic ischiopagus CT. The differential diagnosis of the type of twining and other entities with caudal duplications is analyzed briefly., (Copyright 2003, Elsevier Science (USA). All rights reserved.)
- Published
- 2003
- Full Text
- View/download PDF
16. [Anatomopathologic and ultrastructural study of cardiac myxomas].
- Author
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Barrios del Valle R, Franco Topete R, Fortoul Van der Goes TI, and Oliva Ramírez EB
- Subjects
- Heart Atria pathology, Heart Neoplasms ultrastructure, Humans, Myxoma ultrastructure, Heart Neoplasms pathology, Myxoma pathology
- Abstract
For clinicians, cardiac myxomas has been a diagnostic challenge. It may simulate a valvular disfunction. The present paper displays the morphologic aspects on 17 patients with the diagnosis of atrial myxoma. In 14 cases the tumor was located in the left atrium. In all the cases stellate shaped cells were seen within a myxoid matrix. The findings in this report support the idea that myxomas are tumors and not thrombi as it has been speculated in the literature. We also consider that the cell of origin of this neoplasm is a pluripotential cell which needs further studies.
- Published
- 1986
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