36 results on '"Franco LC"'
Search Results
2. PURPURA TROPOCITOPÊNICA TROMBÓTICA POSSIVELMENTE ASSOCIADA À VACINA CONTRA COVID-19
- Author
-
Gavron, T, primary, Beleze, MAB, additional, Trigo, FC, additional, and Franco, LC, additional
- Published
- 2021
- Full Text
- View/download PDF
3. DIFICULDADE DIAGNÓSTICA DE LEUCEMIA LINFOIDE AGUDA/LINFOMA LINFOBLÁSTICO: RELATO DE CASO.
- Author
-
Beleze, MAB, primary, Gavron, T, additional, Trigo, FC, additional, and Franco, LC, additional
- Published
- 2021
- Full Text
- View/download PDF
4. POLIMORFISMO DE GENES DA GLUTATIONA-S-TRANFERASE E SUSCEPTIBILIDADE AO ESTRESSE OXIDATIVO EM PACIENTES COM LINFOMA HODGKIN E NÃO-HODGKIN
- Author
-
Franco, LC, primary, Oliveira, CEC, additional, and Pinho, RA, additional
- Published
- 2021
- Full Text
- View/download PDF
5. The NORSe: changing the way we communicate
- Author
-
Mehet, J, primary, Sanchez Franco, LC, additional, Gascon Conde, I, additional, Sanchez Campoy, A, additional, Thimmaiah, R, additional, Eldessouky, A, additional, Uzoigwe, CE, additional, and Smith, R, additional
- Published
- 2018
- Full Text
- View/download PDF
6. Biological material exposure during external handle of health services waste.
- Author
-
de Barros DX, Franco LC, Tipple AFV, Barbosa MA, and e Souza ACS
- Published
- 2010
7. Validation of a qualitative real-time PCR assay for the detection of Candida auris in hospital inpatient screening.
- Author
-
Franco LC, Ahmed M, Kendra CG, Sperling RM, Van Benten K, Lavik J-P, Emery CL, Relich RF, and Gavina K
- Subjects
- Humans, Mass Screening methods, Inpatients, Molecular Diagnostic Techniques methods, Molecular Diagnostic Techniques standards, Hospitals, Candida genetics, Candida isolation & purification, DNA, Fungal genetics, Real-Time Polymerase Chain Reaction methods, Sensitivity and Specificity, Candidiasis diagnosis, Candidiasis microbiology, Candida auris genetics
- Abstract
Candida auris is a multidrug-resistant opportunistic fungal pathogen capable of causing serious infections and healthcare-associated outbreaks. Screening for colonization with C. auris has become routine and is recommended in many hospitals and healthcare facilities as an infection control and prevention strategy. Subsequently, and since there are currently no FDA-approved tests for this purpose, clinical microbiology laboratories have become responsible for developing protocols to detect C. auris using axial and inguinal screening swabs. In a College of American Pathologists-accredited large academic healthcare center setting, we implemented a laboratory-developed nucleic-acid amplification test for the detection of C. auris DNA. Our test validation evaluated the performance of the DiaSorin C. auris primer set used in a real-time qualitative PCR assay on the LIAISON MDX thermocycler with the Simplexa Universal Disc. The assay was highly sensitive and specific, with a limit of detection of 1-2 CFU/reaction, with no observed cross-reactivity with other Candida spp., bacterial skin commensal organisms or commonly encountered viruses. When run in parallel with a culture-based detection method, the PCR assay was 100% sensitive and specific. The assay was precise, with low variability between replicates within and between runs. Lastly, pre-analytical factors, including swab storage time, temperature, and transport media, were assessed and found to have no significant effect on the detection of C. auris at variable concentrations. Taken together, this study expands the available options for nucleic acid detection of C. auris and characterizes pre-analytical factors for implementation in both high- and low-volume laboratory settings., Importance: This study overviews the validation and implementation of a molecular screening tool for the detection of Candida auris in a College of American Pathologist-accredited clinical laboratory. This molecular laboratory-developed test is both highly sensitive and specific and has significant health-system cost-savings associated with significantly reduced turn-around-time compared to traditional standard-of-care culture-based work up. This method and workflow is of interest to support clinical microbiology diagnostics and to help aid in hospital inpatient, and infection prevention control screening., Competing Interests: The authors declare no conflict of interest.
- Published
- 2024
- Full Text
- View/download PDF
8. Four new records of Sipuncula (Annelida) from Northern Brazil.
- Author
-
Franco LC, Magalhes WF, Guimares LS, Oliveira D, Rocha EC, and Kawauchi GY
- Subjects
- Animals, Brazil, Annelida, Nematoda
- Abstract
There are very few taxonomic studies of Brazilian sipunculan worms, and the species occurring along the northern coasts are largely understudied. We report herein the occurrence of four shallow water species along the intertidal zones of Bahia and Pernambuco states. Antillesoma antillarum (Grbe & Oersted, 1858) occurs from underneath beach rocks or within sandstone reefs and may reach high densities. The large species Sipunculus (Sipunculus) nudus Linnaeus, 1766, Sipunculus polymyotus Fisher, 1947, and Xenosiphon branchiatus (Fischer, 1895) were collected in sandy-mud, low-energy intertidal environments. For the first time, we report that sipunculans are collected and used as fishing bait by local fishermen in the Western Atlantic. All species are fully described, including notes on their external and internal anatomy.
- Published
- 2024
- Full Text
- View/download PDF
9. Molecular point-of-care devices for the diagnosis of infectious diseases in resource-limited settings - A review of the current landscape, technical challenges, and clinical impact.
- Author
-
Gavina K, Franco LC, Khan H, Lavik JP, and Relich RF
- Subjects
- Humans, Resource-Limited Settings, Point-of-Care Testing, Laboratories, Point-of-Care Systems, Communicable Diseases diagnosis
- Abstract
Molecular point-of-care (POC) tests offer high sensitivity, rapid turnaround times, relative ease of use, and the convenience of laboratory-grade testing in the absence of formal laboratory spaces and equipment, making them appealing options for infectious disease diagnosis in resource-limited settings. In this review, we discuss the role and potential of molecular POC tests in resource-limited settings and their associated logistical challenges. We discuss U.S. Food and Drug Administration approval, Clinical Laboratory Improvement Amendments complexity levels, and the REASSURED criteria as a starting point for assessing options currently available inside and outside of the United States. We then present POC tests currently in research and development phases that have potential for commercialization and implementation in limited-resource settings. Finally, we review published studies that have assessed the clinical impact of molecular POC testing in limited- and moderate-resource settings., Competing Interests: Declaration of Competing Interest Drs. Kenneth Gavina, Lauren C. Franco, Haseeba Khan, and JP-Lavik declare no conflicts of interest. Dr. Ryan F. Relich receives research support from bioMerieux/BioFire, Cepheid, COPAN Diagnostics, QIAGEN, and Roche Diagnostics; however, none of these commercial entities’ products, services, research data, etc. were included in this manuscript., (Copyright © 2023. Published by Elsevier B.V.)
- Published
- 2023
- Full Text
- View/download PDF
10. Standardization of SARS-CoV-2 Cycle Threshold Values: Multisite Investigation Evaluating Viral Quantitation across Multiple Commercial COVID-19 Detection Platforms.
- Author
-
Gavina K, Franco LC, Robinson CM, Hymas W, Lei GS, Sinclair W, Hall T, Carlquist J, Lavik JP, Emery CL, Heaton PR, Hillyard D, Lopransi BK, and Relich RF
- Subjects
- Humans, United States, SARS-CoV-2 genetics, COVID-19 Testing, Pandemics, Clinical Laboratory Techniques methods, Reference Standards, COVID-19 diagnosis
- Abstract
The demand for testing during the coronavirus disease 2019 (COVID-19) pandemic has resulted in the production of several different commercial platforms and laboratory-developed assays for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This has created several challenges, including, but not limited to, the standardization of diagnostic testing, utilization of cycle threshold ( C
T ) values for quantitation and clinical interpretation, and data harmonization. Using reference standards consisting of a linear range of SARS-CoV-2 concentrations quantitated by viral culture-based methods and droplet digital PCR, we investigated the commutability and standardization of SARS-CoV-2 quantitation across different laboratories in the United States. We assessed SARS-CoV-2 CT values generated on multiple reverse transcription-PCR (RT-PCR) platforms and analyzed PCR efficiencies, linearity, gene targets, and CT value agreement. Our results demonstrate the inappropriateness of using SARS-CoV-2 CT values without established standards for viral quantitation. Further, we emphasize the importance of using reference standards and controls validated to independent assays, to compare results across different testing platforms and move toward better harmonization of COVID-19 quantitative test results. IMPORTANCE From the onset of the COVID-19 pandemic, the demand for SARS-CoV-2 testing has resulted in an explosion of analytical tests with very different approaches and designs. The variability in testing modalities, compounded by the lack of available commercial reference materials for standardization early in the pandemic, has led to several challenges regarding data harmonization for viral quantitation. In this study, we assessed multiple commercially available RT-PCR platforms across different laboratories within the United States using standardized reference materials characterized by viral culture methods and droplet digital PCR. We observed variability in the results generated by different instruments and laboratories, further emphasizing the importance of utilizing validated reference standards for quantitation, to better harmonize SARS-CoV-2 test results.- Published
- 2023
- Full Text
- View/download PDF
11. Association Between Social Pensions With Depression, Social, and Health Behaviors Among Poor Older Individuals in Colombia.
- Author
-
Hessel P, Bermeo López LM, López Franco LC, Ham A, Pinilla-Roncancio M, and González-Uribe C
- Subjects
- Aged, Colombia, Female, Humans, Income, Male, Poverty economics, Rural Population statistics & numerical data, Depression epidemiology, Health Behavior, Pensions statistics & numerical data, Poverty statistics & numerical data, Retirement economics
- Abstract
Objectives: Many low- and middle-income countries have introduced social pensions to alleviate extreme poverty and improve the well-being of older individuals. However, evidence remains inconclusive about the potential effects of such programs on mental health, social, and health behaviors., Methods: Data for individuals aged 60 or older came from the nationally representative Encuesta Nacional de Salud, Bienestar y Envejeciamiento survey in Colombia 2015 (N = 9,456). We used propensity score matching to estimate the association between the country's social pension program (Colombia Mayor) with depression, self-rated health, food insecurity, alcohol consumption, social participation, and labor force participation., Results: Results show that receiving the program does not significantly affect the likelihood of suffering from depression or self-rated health among either men or women. However, receiving the program is associated with significant reductions in the likelihood of experiencing food insecurity and significant increases in the likelihood of participating socially. Among women, receiving the program is associated with significant reduction in the likelihood of participating in the labor force., Discussion: The absence of a measurable effect on depression and self-rated health may be explained, at least partly, by the program's comparatively small cash benefit and the sharing of resources with other family members. Policymakers should assess possibilities to maximize the health and social benefits of social pensions., (© The Author(s) 2020. Published by Oxford University Press on behalf of The Gerontological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Published
- 2021
- Full Text
- View/download PDF
12. Unexpected Heparin-Induced Thrombocytopenia While Bridging.
- Author
-
Uzoigwe CE, Sanchez Franco LC, and Ali O
- Subjects
- Anticoagulants adverse effects, Heparin adverse effects, Humans, Thrombocytopenia chemically induced, Thrombocytopenia diagnosis
- Published
- 2020
- Full Text
- View/download PDF
13. A microbiological survey of handwashing sinks in the hospital built environment reveals differences in patient room and healthcare personnel sinks.
- Author
-
Franco LC, Tanner W, Ganim C, Davy T, Edwards J, and Donlan R
- Subjects
- Carbapenem-Resistant Enterobacteriaceae isolation & purification, Hospitals, Humans, Pseudomonas aeruginosa isolation & purification, Hand Disinfection, Patients' Rooms, Personnel, Hospital
- Abstract
Handwashing sinks and their associated premise plumbing are an ideal environment for pathogen-harboring biofilms to grow and spread throughout facilities due to the connected system of wastewater plumbing. This study was designed to understand the distribution of pathogens and antibiotic resistant organisms (ARO) within and among handwashing sinks in healthcare settings, using culture-dependent methods to quantify Pseudomonas aeruginosa, opportunistic pathogens capable of growth on a cefotaxime-containing medium (OPP-C), and carbapenem-resistant Enterobacteriaceae (CRE). Isolates from each medium identified as P. aeruginosa or Enterobacteriaceae were tested for susceptibility to aztreonam, ceftazidime, and meropenem; Enterobacteriaceae were also tested against ertapenem and cefotaxime. Isolates exhibiting resistance or intermediate resistance were designated ARO. Pathogens were quantified at different locations within handwashing sinks and compared in quantity and distribution between healthcare personnel (HCP) and patient room (PR) sinks. ARO were compared between samples within a sink (biofilm vs planktonic samples) and between sink types (HCP vs. PR). The drain cover was identified as a reservoir within multiple sinks that was often colonized by pathogens despite daily sink cleaning. P. aeruginosa and OPP-C mean log
10 CFU/cm2 counts were higher in p-trap and tail pipe biofilm samples from HCP compared to PR sinks (2.77 ± 2.39 vs. 1.23 ± 1.62 and 5.27 ± 1.10 vs. 4.74 ± 1.06) for P. aeruginosa and OPP-C, respectively. P. aeruginosa and OPP-C mean log10 CFU/ml counts were also higher (p < 0.05) in HCP compared to PR sinks p-trap water (2.21 ± 1.52 vs. 0.89 ± 1.44 and 3.87 ± 0.78 vs. 3.21 ± 1.11) for P. aeruginosa and OPP-C, respectively. However, a greater percentage of ARO were recovered from PR sinks compared to HCP sinks (p < 0.05) for Enterobacteriaceae (76.4 vs. 32.9%) and P. aeruginosa (25.6 vs. 0.3%). This study supports previous work citing that handwashing sinks are reservoirs for pathogens and ARO and identifies differences in pathogen and ARO quantities between HCP and PR sinks, despite the interconnected premise plumbing.- Published
- 2020
- Full Text
- View/download PDF
14. Oral Anticoagulants, Proton Pump Inhibitors, and Fracture.
- Author
-
Uzoigwe CE, Sanchez Franco LC, and Gascon Conde I
- Subjects
- Anticoagulants adverse effects, Humans, Proton Pump Inhibitors adverse effects, Atrial Fibrillation, Fractures, Bone
- Published
- 2020
- Full Text
- View/download PDF
15. Poverty is the real enemy of women's health.
- Author
-
Uzoigwe CE and Sanchez Franco LC
- Abstract
Competing Interests: Competing interests: None declared.
- Published
- 2019
- Full Text
- View/download PDF
16. Adverse birth outcomes associated with Zika virus exposure during pregnancy in São José do Rio Preto, Brazil.
- Author
-
Nogueira ML, Nery Júnior NRR, Estofolete CF, Bernardes Terzian AC, Guimarães GF, Zini N, Alves da Silva R, Dutra Silva GC, Junqueira Franco LC, Rahal P, Bittar C, Carneiro B, Vasconcelos PFC, Freitas Henriques D, Barbosa DMU, Lopes Rombola P, de Grande L, Negri Reis AF, Palomares SA, Wakai Catelan M, Cruz LEAA, Necchi SH, Mendonça RCV, Penha Dos Santos IN, Alavarse Caron SB, Costa F, Bozza FA, Soares de Souza A, Brandão de Mattos CC, de Mattos LC, Vasilakis N, Oliani AH, Vaz Oliani DCM, and Ko AI
- Subjects
- Adult, Brazil, Female, Humans, Infant, Newborn, Phylogeny, Pregnancy, Young Adult, Zika Virus classification, Zika Virus genetics, Fetal Diseases virology, Pregnancy Complications, Infectious virology, Zika Virus isolation & purification, Zika Virus Infection complications
- Abstract
Objectives: We aimed to report the first 54 cases of pregnant women infected by Zika virus (ZIKV) and their virologic and clinical outcomes, as well as their newborns' outcomes, in 2016, after the emergence of ZIKV in dengue-endemic areas of São Paulo, Brazil., Methods: This descriptive study was performed from February to October 2016 on 54 quantitative real-time PCR ZIKV-positive pregnant women identified by the public health authority of São José do Rio Preto, São Paulo, Brazil. The women were followed and had clinical and epidemiologic data collected before and after birth. Adverse outcomes in newborns were analysed and reported. Urine or blood samples from newborns were collected to identify ZIKV infection by reverse transcription PCR (RT-PCR)., Results: A total of 216 acute Zika-suspected pregnant women were identified, and 54 had the diagnosis confirmed by RT-PCR. None of the 54 women miscarried. Among the 54 newborns, 15 exhibited adverse outcomes at birth. The highest number of ZIKV infections occurred during the second and third trimesters. No cases of microcephaly were reported, though a broad clinical spectrum of outcomes, including lenticulostriate vasculopathy, subependymal cysts, and auditory and ophthalmologic disorders, were identified. ZIKV RNA was detected in 18 of 51 newborns tested and in eight of 15 newborns with adverse outcomes., Conclusions: Although other studies have associated many newborn outcomes to ZIKV infection during pregnancy, these same adverse outcomes were rare or nonexistent in this study. The clinical presentation the newborns we studied was mild compared to other reports, suggesting that there is significant heterogeneity in congenital Zika infection., (Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2018
- Full Text
- View/download PDF
17. Male pattern hair loss: Taking one for the team: The selfless gene.
- Author
-
Uzoigwe CE, Sanchez Franco LC, Gascon Conde I, and Sanchez Campoy A
- Subjects
- Alleles, Female, Humans, Male, Marriage, Models, Theoretical, Alopecia genetics, Genetic Fitness, Paternal Age, Selection, Genetic
- Abstract
Male pattern hair loss (MPHL) is exceedingly common. It is characterised by onset in early adulthood and progression with age. It has a strong heritable component. The reason for its existence remains unexplained. Given that MPHL is progressive and has its earliest manifestations in young adults it may be a barometer of age. Here we suggest that MPHL may have atavistically allowed women in our species and ancestor species to select younger (but not necessarily the youngest) adult mates. Evidence suggests that conceptions by younger fathers are more likely to lead to live births and less likely to result in miscarriage. Further children fathered by younger men may have improved health and be less likely to suffer from a number of co-morbidities. This is collectively known as the "paternal age affect". Hence the selection of younger males mediated by the MPHL may improve the fitness of the population and of the species at the expense of the individual. Indeed MPHL may have been an evolutionary "nudge" directing women to favour younger partners. It is conceivable that for a species whose success is predicated upon co-operation, collaboration and altruism the gene cannot be exclusively selfish and must have a selfless allele., (Copyright © 2018 Elsevier Ltd. All rights reserved.)
- Published
- 2018
- Full Text
- View/download PDF
18. Night shifts: chronotype and social jetlag.
- Author
-
Uzoigwe CE and Sanchez Franco LC
- Subjects
- Humans, Work Schedule Tolerance physiology, Circadian Rhythm physiology, Jet Lag Syndrome physiopathology, Medical Staff, Hospital organization & administration
- Abstract
Competing Interests: Competing interests: None declared.
- Published
- 2018
- Full Text
- View/download PDF
19. Cr(VI) reduction and physiological toxicity are impacted by resource ratio in Desulfovibrio vulgaris.
- Author
-
Franco LC, Steinbeisser S, Zane GM, Wall JD, and Fields MW
- Subjects
- Anaerobiosis, Desulfovibrio vulgaris growth & development, Microbial Viability drug effects, Oxidation-Reduction, Sulfates metabolism, Temperature, Carcinogens, Environmental metabolism, Carcinogens, Environmental toxicity, Chromium metabolism, Chromium toxicity, Desulfovibrio vulgaris drug effects, Desulfovibrio vulgaris metabolism
- Abstract
Desulfovibrio spp. are capable of heavy metal reduction and are well-studied systems for understanding metal fate and transport in anaerobic environments. Desulfovibrio vulgaris Hildenborough was grown under environmentally relevant conditions (i.e., temperature, nutrient limitation) to elucidate the impacts on Cr(VI) reduction on cellular physiology. Growth at 20 °C was slower than 30 °C and the presence of 50 μM Cr(VI) caused extended lag times for all conditions, but once growth resumed the growth rate was similar to that without Cr(VI). Cr(VI) reduction rates were greatly diminished at 20 °C for both 50 and 100 μM Cr(VI), particularly for the electron acceptor limited (EAL) condition in which Cr(VI) reduction was much slower, the growth lag much longer (200 h), and viability decreased compared to balanced (BAL) and electron donor limited (EDL) conditions. When sulfate levels were increased in the presence of Cr(VI), cellular responses improved via a shorter lag time to growth. Similar results were observed between the different resource (donor/acceptor) ratio conditions when the sulfate levels were normalized (10 mM), and these results indicated that resource ratio (donor/acceptor) impacted D. vulgaris response to Cr(VI) and not merely sulfate limitation. The results suggest that temperature and resource ratios greatly impacted the extent of Cr(VI) toxicity, Cr(VI) reduction, and the subsequent cellular health via Cr(VI) influx and overall metabolic rate. The results also emphasized the need to perform experiments at lower temperatures with nutrient limitation to make accurate predictions of heavy metal reduction rates as well as physiological states in the environment.
- Published
- 2018
- Full Text
- View/download PDF
20. CDK9: A key player in cancer and other diseases.
- Author
-
Franco LC, Morales F, Boffo S, and Giordano A
- Subjects
- Animals, Cardiovascular Diseases genetics, Cyclin T genetics, Cyclin T metabolism, Cyclin-Dependent Kinase 9 genetics, Humans, Neoplasm Proteins genetics, Neoplasms genetics, Positive Transcriptional Elongation Factor B genetics, Positive Transcriptional Elongation Factor B metabolism, Transcription Elongation, Genetic, Virus Diseases genetics, Cardiovascular Diseases metabolism, Cyclin-Dependent Kinase 9 metabolism, Neoplasm Proteins metabolism, Neoplasms metabolism, Virus Diseases metabolism
- Abstract
Cyclin-Dependent Kinase 9 (CDK9) is part of a functional diverse group of enzymes responsible for cell cycle control and progression. It associates mainly with Cyclin T1 and forms the Positive Transcription Elongation Factor b (p-TEFb) complex responsible for regulation of transcription elongation and mRNA maturation. Recent studies have highlighted the importance of CDK9 in many relevant pathologic processes, like cancer, cardiovascular diseases, and viral replication. Herein we provide an overview of the different pathways in which CDK9 is directly and indirectly involved., (© 2017 Wiley Periodicals, Inc.)
- Published
- 2018
- Full Text
- View/download PDF
21. Delivery route determines the presence of immune complexes on umbilical cord erythrocytes.
- Author
-
de Lima A, Franco LC, Sarmiento A, and González JM
- Subjects
- Adult, Apgar Score, Cesarean Section, Female, Gestational Age, Humans, Infant, Newborn, Male, Pregnancy, Young Adult, Antigen-Antibody Complex metabolism, Delivery, Obstetric methods, Erythrocytes immunology, Erythrocytes metabolism, Fetal Blood immunology, Fetal Blood metabolism
- Abstract
Objective: Umbilical cord blood offers a unique opportunity to study the basal level of immunoglobulin complexes. This study aims to determine the presence of immune complexes and complement deposition on erythrocytes from umbilical cord blood from normal, full-term pregnancies., Methods: In vitro pre-formed IgA, IgG, and IgM complexes were used as positive control for flow cytometry detection, and for C3d deposition. Blood samples (34) of umbilical cord blood taken from vaginal and cesarean deliveries were tested for the presence of immunoglobulin complexes., Results: Fourteen samples from vaginal deliveries and 20 samples from cesarean deliveries were assessed. IgG and IgM complexes were detected on erythrocytes, whereas no IgA complexes or complement deposition was observed. Interestingly, the percentage of IgG complexes was higher on erythrocytes from vaginal delivery samples compared to those from cesarean deliveries. No other associations between immune complexes and other maternal or newborn variables were found., Conclusions: IgG and IgM complexes seem to be normally present on umbilical cord erythrocytes. Erythrocytes from vaginal deliveries have a higher percentage of IgG complexes present compared to that from cesarean deliveries. Since no C3d activity was detected, these complexes are non-pathological and should be part of the newborn's initial innate immune response.
- Published
- 2017
- Full Text
- View/download PDF
22. Establishment and cryptic transmission of Zika virus in Brazil and the Americas.
- Author
-
Faria NR, Quick J, Claro IM, Thézé J, de Jesus JG, Giovanetti M, Kraemer MUG, Hill SC, Black A, da Costa AC, Franco LC, Silva SP, Wu CH, Raghwani J, Cauchemez S, du Plessis L, Verotti MP, de Oliveira WK, Carmo EH, Coelho GE, Santelli ACFS, Vinhal LC, Henriques CM, Simpson JT, Loose M, Andersen KG, Grubaugh ND, Somasekar S, Chiu CY, Muñoz-Medina JE, Gonzalez-Bonilla CR, Arias CF, Lewis-Ximenez LL, Baylis SA, Chieppe AO, Aguiar SF, Fernandes CA, Lemos PS, Nascimento BLS, Monteiro HAO, Siqueira IC, de Queiroz MG, de Souza TR, Bezerra JF, Lemos MR, Pereira GF, Loudal D, Moura LC, Dhalia R, França RF, Magalhães T, Marques ET Jr, Jaenisch T, Wallau GL, de Lima MC, Nascimento V, de Cerqueira EM, de Lima MM, Mascarenhas DL, Neto JPM, Levin AS, Tozetto-Mendoza TR, Fonseca SN, Mendes-Correa MC, Milagres FP, Segurado A, Holmes EC, Rambaut A, Bedford T, Nunes MRT, Sabino EC, Alcantara LCJ, Loman NJ, and Pybus OG
- Subjects
- Americas epidemiology, Basic Reproduction Number, Brazil epidemiology, Genetic Variation, Genome, Viral genetics, Humans, Microcephaly epidemiology, Microcephaly virology, Molecular Epidemiology, Phylogeography, Spatio-Temporal Analysis, Zika Virus genetics, Zika Virus Infection epidemiology, Zika Virus isolation & purification, Zika Virus Infection transmission, Zika Virus Infection virology
- Abstract
Transmission of Zika virus (ZIKV) in the Americas was first confirmed in May 2015 in northeast Brazil. Brazil has had the highest number of reported ZIKV cases worldwide (more than 200,000 by 24 December 2016) and the most cases associated with microcephaly and other birth defects (2,366 confirmed by 31 December 2016). Since the initial detection of ZIKV in Brazil, more than 45 countries in the Americas have reported local ZIKV transmission, with 24 of these reporting severe ZIKV-associated disease. However, the origin and epidemic history of ZIKV in Brazil and the Americas remain poorly understood, despite the value of this information for interpreting observed trends in reported microcephaly. Here we address this issue by generating 54 complete or partial ZIKV genomes, mostly from Brazil, and reporting data generated by a mobile genomics laboratory that travelled across northeast Brazil in 2016. One sequence represents the earliest confirmed ZIKV infection in Brazil. Analyses of viral genomes with ecological and epidemiological data yield an estimate that ZIKV was present in northeast Brazil by February 2014 and is likely to have disseminated from there, nationally and internationally, before the first detection of ZIKV in the Americas. Estimated dates for the international spread of ZIKV from Brazil indicate the duration of pre-detection cryptic transmission in recipient regions. The role of northeast Brazil in the establishment of ZIKV in the Americas is further supported by geographic analysis of ZIKV transmission potential and by estimates of the basic reproduction number of the virus.
- Published
- 2017
- Full Text
- View/download PDF
23. Obesity and Cesarean Section: Bacteria or Birth Interval?
- Author
-
Uzoigwe CE, Porter KJ, and Sanchez Franco LC
- Subjects
- Adolescent, Bacteria, Body Mass Index, Child, Female, Humans, Obesity, Pregnancy, Birth Intervals, Cesarean Section
- Published
- 2017
- Full Text
- View/download PDF
24. Frequency of anti- Toxoplasma gondii IgA, IgM, and IgG antibodies in high-risk pregnancies, in Brazil.
- Author
-
Murata FH, Ferreira MN, Camargo NS, Santos GS, Spegiorin LC, Silveira-Carvalho AP, Pereira-Chioccola VL, Mattos LC, and Mattos CC
- Subjects
- Adolescent, Adult, Brazil, Enzyme-Linked Immunosorbent Assay, Female, Humans, Pregnancy, Pregnancy Complications, Parasitic immunology, Pregnancy, High-Risk immunology, Young Adult, Antibodies, Protozoan blood, Immunoglobulin A blood, Immunoglobulin G blood, Immunoglobulin M blood, Pregnancy Complications, Parasitic diagnosis, Toxoplasma immunology
- Abstract
Introduction: Toxoplasmosis during pregnancy can be severe; thus, it is essential to diagnose the disease via serological tests., Methods: An enzyme-linked immunosorbent assay (ELISA) was used to investigate anti-Toxoplasma gondii immunoglobulin A (IgA), M (IgM) and G (IgG) antibodies in 62 high-risk pregnant women., Results: Forty-three (69.4%) women were positive for IgA, 31 (50%) for IgG, and 57 (91.9%) for IgM; 4 (6,5%) were positive for IgA but negative for IgM; 10 (16.1%) were negative for IgA and IgM but positive for IgG., Conclusions: Testing for these antibodies can help diagnose infection in pregnant women, thereby contributing to clinical management.
- Published
- 2016
- Full Text
- View/download PDF
25. Maternal and Neonatal Mortality After Cesarean Delivery.
- Author
-
Uzoigwe C, Sanchez Franco LC, and Gascon Conde I
- Subjects
- Female, Humans, Pregnancy, Cesarean Section statistics & numerical data, Infant Mortality trends, Maternal Mortality trends
- Published
- 2016
- Full Text
- View/download PDF
26. New anti-resorptives and antibody mediated anti-resorptive therapy.
- Author
-
Farrier AJ, Sanchez Franco LC, Shoaib A, Gulati V, Johnson N, Uzoigwe CE, and Choudhury MZ
- Subjects
- Adaptor Proteins, Signal Transducing, Antibodies, Monoclonal, Humanized pharmacology, Bisphosphonate-Associated Osteonecrosis of the Jaw etiology, Bone Density Conservation Agents pharmacology, Bone Morphogenetic Proteins antagonists & inhibitors, Bone Neoplasms drug therapy, Bone Neoplasms secondary, Cost-Benefit Analysis, Denosumab pharmacology, Diphosphonates therapeutic use, Drug Approval, Femoral Fractures chemically induced, Genetic Markers, Humans, Hypocalcemia chemically induced, Osteoporosis drug therapy, Osteoporotic Fractures drug therapy, Practice Guidelines as Topic, Quality-Adjusted Life Years, RANK Ligand antagonists & inhibitors, Antibodies, Monoclonal, Humanized therapeutic use, Bone Density Conservation Agents therapeutic use, Bone Resorption drug therapy, Denosumab therapeutic use
- Abstract
Unlabelled: The ageing population and an increase in both the incidence and prevalence of cancer pose a healthcare challenge, some of which is borne by the orthopaedic community in the form of osteoporotic fractures and metastatic bone disease. In recent years there has been an increasing understanding of the pathways involved in bone metabolism relevant to osteoporosis and metastases in bone. Newer therapies may aid the management of these problems. One group of drugs, the antibody mediated anti-resorptive therapies (AMARTs) use antibodies to block bone resorption pathways. This review seeks to present a synopsis of the guidelines, pharmacology and potential pathophysiology of AMARTs and other new anti-resorptive drugs. We evaluate the literature relating to AMARTs and new anti-resorptives with special attention on those approved for use in clinical practice. Denosumab, a monoclonal antibody against Receptor Activator for Nuclear Factor Kappa-B Ligand. It is the first AMART approved by the National Institute for Health and Clinical Excellence and the US Food and Drug Administration. Other novel anti-resorptives awaiting approval for clinical use include Odanacatib. Denosumab is indicated for the treatment of osteoporosis and prevention of the complications of bone metastases. Recent evidence suggests, however, that denosumab may have an adverse event profile similar to bisphosphonates, including atypical femoral fractures. It is, therefore, essential that orthopaedic surgeons are conversant with these medications and their safe usage., Take Home Message: Denosumab has important orthopaedic indications and has been shown to significantly reduce patient morbidity in osteoporosis and metastatic bone disease., (©2016 The British Editorial Society of Bone & Joint Surgery.)
- Published
- 2016
- Full Text
- View/download PDF
27. Iatrogenic greenhouse gases: the role of anaesthetic agents.
- Author
-
Uzoigwe CE, Sanchez Franco LC, and Forrest MD
- Subjects
- Climate Change, Greenhouse Effect, Humans, Stratospheric Ozone chemistry, Anesthetics chemistry, Fluorocarbons chemistry, Gases chemistry
- Abstract
The contribution of health-care activity to climate change is not negligible and is increasing. Anaesthetic greenhouse gases, in particular the fluranes, have a much more potent global warming capacity, volume for volume, than carbon dioxide, but their emissions remain completely unregulated.
- Published
- 2016
- Full Text
- View/download PDF
28. Prematurity and Low Birth Weight did not Correlate with Anti-Toxoplasma gondii Maternal Serum Profiles--a Brazilian Report.
- Author
-
Fochi MM, Baring S, Spegiorin LC, Vaz-Oliani DC, Galão EA, Oliani AH, de Mattos LC, and de Mattos CC
- Subjects
- Abortion, Spontaneous immunology, Adult, Brazil, Female, Humans, Infant, Newborn, Infant, Premature, Pregnancy, Risk Factors, Toxoplasmosis immunology, Abortion, Spontaneous etiology, Antibodies, Protozoan blood, Infant, Low Birth Weight immunology, Toxoplasma immunology, Toxoplasmosis complications
- Abstract
Gestational Toxoplasma gondii infection is considered a major risk factor for miscarriage, prematurity and low birth weight in animals. However, studies focusing on this topic in humans are scarce. The objective of this study is to determine whether anti-Toxoplasma gondii maternal serum profiles correlate prematurity and low birth weight in humans. The study examined 213 pregnant women seen at the High-Risk Pregnancy Hospital de Base, São José do Rio Preto, São Paulo, Brazil. All serological profiles (IgM-/IgG+; IgM-/IgG-; IgM+/IgG+) were determined by ELISA commercial kits. Maternal age, gestational age and weight of the newborn at birth were collected and recorded in the Statement of Live Birth. Prematurity was defined as gestational age <37 weeks and low birth weight ≤ 2499 grams. The t-test was used to compare values (p < 0.05). The mean maternal age was 27.6±6.6 years. Overall, 56.3% (120/213) of the women studied were IgM-/IgG+, 36.2% (77/213) were IgM-/IgG- and 7.5% (16/213) were IgM+/IgG+. The average age of the women with serological profile IgM+/IgG+ (22.3±3.9 years) was different from women with the profile IgM-/IgG+ (27.9±6.7 years, p = 0.0011) and IgM-/IgG- (27.9±6.4 years, p = 0.0012). There was no statistically significant difference between the different serological profiles in relation to prematurity (p = 0.6742) and low birth weight (p = 0.7186). The results showed that prematurity and low birth weight did not correlate with anti-Toxoplasma gondii maternal serum profiles.
- Published
- 2015
- Full Text
- View/download PDF
29. Genome sequence of Exiguobacterium antarcticum B7, isolated from a biofilm in Ginger Lake, King George Island, Antarctica.
- Author
-
Carneiro AR, Ramos RT, Dall'Agnol H, Pinto AC, de Castro Soares S, Santos AR, Guimarães LC, Almeida SS, Baraúna RA, das Graças DA, Franco LC, Ali A, Hassan SS, Nunes CI, Barbosa MS, Fiaux KK, Aburjaile FF, Barbosa EG, Bakhtiar SM, Vilela D, Nóbrega F, dos Santos AL, Carepo MS, Azevedo V, Schneider MP, Pellizari VH, and Silva A
- Subjects
- Antarctic Regions, Bacillales isolation & purification, Bacillales physiology, Biofilms growth & development, Fresh Water microbiology, Islands, Lakes, Molecular Sequence Data, Bacillales genetics, DNA, Bacterial chemistry, DNA, Bacterial genetics, Genome, Bacterial, Sequence Analysis, DNA
- Abstract
Exiguobacterium antarcticum is a psychotropic bacterium isolated for the first time from microbial mats of Lake Fryxell in Antarctica. Many organisms of the genus Exiguobacterium are extremophiles and have properties of biotechnological interest, e.g., the capacity to adapt to cold, which make this genus a target for discovering new enzymes, such as lipases and proteases, in addition to improving our understanding of the mechanisms of adaptation and survival at low temperatures. This study presents the genome of E. antarcticum B7, isolated from a biofilm sample of Ginger Lake on King George Island, Antarctic peninsula.
- Published
- 2012
- Full Text
- View/download PDF
30. Canalis sinuosus: a rare anatomical variation.
- Author
-
Neves FS, Crusoé-Souza M, Franco LC, Caria PH, Bonfim-Almeida P, and Crusoé-Rebello I
- Subjects
- Bone Transplantation, Cone-Beam Computed Tomography methods, Female, Humans, Maxilla diagnostic imaging, Maxilla surgery, Maxillary Nerve diagnostic imaging, Middle Aged, Nasal Cavity diagnostic imaging, Palate, Hard abnormalities, Palate, Hard diagnostic imaging, Maxilla abnormalities, Maxillary Nerve abnormalities, Nasal Cavity abnormalities
- Abstract
The infraorbital canal issues a small branch on its lateral face close to its midpoint to allow passage of the anterior superior alveolar nerve. This small canal, sometimes called the canalis sinuosus, runs forward and downward to the inferior wall of the orbit, lateral to the infraorbital canal and medially bent to the anterior wall of the maxillary sinus, passing below the infraorbital foramen. Anatomical variations in the maxilla are rarely described in the literature and, in most cases, are related to the nasopalatine canal. This article describes a rare anatomical variation of the presence of a bilateral accessory canal extending from the nasal cavity lateral wall to an accessory foramen located on the hard palate, adjacent to the maxillary lateral incisor observed in cone beam computed tomography (CBCT) images. This case is an anatomical variation of the anterior superior alveolar nerve (canalis sinuosus). Identification of individual anatomical variations, especially on CBCT, may help the surgeon to avoid injuries to nerves during implant placement.
- Published
- 2012
- Full Text
- View/download PDF
31. Additional aberrations of the ETV6 and RUNX1 genes have no prognostic impact in 229 t(12;21)(p13;q22)-positive B-cell precursor acute lymphoblastic leukaemias treated according to the NOPHO-ALL-2000 protocol.
- Author
-
Barbany G, Andersen MK, Autio K, Borgström G, Franco LC, Golovleva I, Heim S, Heinonen K, Hovland R, Johansson B, Johannsson JH, Kjeldsen E, Nordgren A, Palmqvist L, and Forestier E
- Subjects
- Adolescent, Child, Child, Preschool, Chromosome Aberrations chemically induced, Chromosomes, Human, Pair 12 genetics, Chromosomes, Human, Pair 21 genetics, Clinical Trials as Topic methods, Cohort Studies, Denmark, Finland, Humans, Iceland, Infant, Mutation physiology, Norway, Precursor B-Cell Lymphoblastic Leukemia-Lymphoma genetics, Prognosis, Sweden, Translocation, Genetic drug effects, ETS Translocation Variant 6 Protein, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Core Binding Factor Alpha 2 Subunit genetics, Precursor B-Cell Lymphoblastic Leukemia-Lymphoma diagnosis, Precursor B-Cell Lymphoblastic Leukemia-Lymphoma therapy, Proto-Oncogene Proteins c-ets genetics, Repressor Proteins genetics
- Published
- 2012
- Full Text
- View/download PDF
32. The ADA*2 allele of the adenosine deaminase gene (20q13.11) and recurrent spontaneous abortions: an age-dependent association.
- Author
-
Nunes DP, Spegiorin LC, Mattos CC, Oliani AH, Vaz-Oliani DC, and Mattos LC
- Subjects
- Abortion, Habitual epidemiology, Abortion, Spontaneous epidemiology, Abortion, Spontaneous genetics, Adult, Age Factors, Brazil epidemiology, Case-Control Studies, Chi-Square Distribution, Female, Genotype, Humans, Pregnancy, Abortion, Habitual genetics, Adenosine Deaminase genetics, Alleles, Polymorphism, Genetic genetics
- Abstract
Objective: Adenosine deaminase acts on adenosine and deoxyadenosine metabolism and modulates the immune response. The adenosine deaminase G22A polymorphism (20q.11.33) influences the level of adenosine deaminase enzyme expression, which seems to play a key role in maintaining pregnancy. The adenosine deaminase 2 phenotype has been associated with a protective effect against recurrent spontaneous abortions in European Caucasian women. The aim of this study was to investigate whether the G22A polymorphism of the adenosine deaminase gene is associated with recurrent spontaneous abortions in Brazilian women., Methods: A total of 311 women were recruited to form two groups: G1, with a history of recurrent spontaneous abortions (N = 129), and G2, without a history of abortions (N = 182). Genomic DNA was extracted from peripheral blood with a commercial kit and PCR-RFLP analysis was used to identify the G22A genetic polymorphism. Fisher's exact test and odds ratio values were used to compare the proportions of adenosine deaminase genotypes and alleles between women with and without a history of recurrent spontaneous abortion (p<0.05). The differences between mean values for categorical data were calculated using unpaired t tests. The Hardy-Weinberg equilibrium was assessed with a chi-square test., Results: Statistically significant differences were identified for the frequencies of adenosine deaminase genotypes and alleles between the G1 and G2 groups when adjusted for maternal age., Conclusions: The results suggest that the adenosine deaminase *2 allele is associated with a low risk for recurrent spontaneous abortions, but this association is dependent on older age.
- Published
- 2011
- Full Text
- View/download PDF
33. Anti-Toxoplasma gondii antibodies in pregnant women and their newborn infants in the region of São José do Rio Preto, São Paulo, Brazil.
- Author
-
Mattos Cde C, Spegiorin LC, Meira Cda S, Silva Tda C, Ferreira AI, Nakashima F, Pereira-Chioccola VL, and Mattos LC
- Subjects
- Antibody Affinity, Brazil epidemiology, Cross-Sectional Studies, Enzyme-Linked Immunosorbent Assay, Female, Fluorescent Antibody Technique, Indirect, Gestational Age, Humans, Risk Factors, Toxoplasmosis transmission, Antibodies, Protozoan immunology, Immunoglobulin G blood, Immunoglobulin M blood, Infant, Newborn immunology, Pregnancy immunology, Toxoplasma immunology
- Abstract
Context and Objective: Toxoplasmosis transmission during pregnancy can cause severe sequelae in fetuses and newborns. Maternal antibodies may be indicators of risk or immunity. The aim here was to evaluate seropositivity for anti-Toxoplasma gondii (anti-T. gondii) immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies and IgG avidity in pregnant women and their newborn infants., Design and Setting: Cross-sectional study in a high-risk pregnancy outpatient clinic., Methods: Serum samples from pregnant women (n = 87) and their respective newborns (n = 87) were evaluated for anti-T. gondii antibodies using indirect immunofluorescence (IIF) (IgM and IgG), enzyme-linked immunosorbent assay (ELISA) (IgG) and an avidity test., Results: Anti-T. gondii antibodies were identified in 64.4% of the serum samples from the mothers and their infants (56/87). Except for two maternal serum samples (2.3%), all others were negative for anti-T. gondii IgM antibodies, using IIF. The results showed that 92.9% of the pregnant women had high IgG avidity indexes (> 30%) and four samples had avidity indexes between 16 and 30%. Two women in the third trimester of pregnancy were positive for anti-T. gondii IgM antibodies; their babies had avidity indexes between 16 and 30%. The avidity indexes of serum from the other 83 newborns were similar to the results from their mothers., Conclusions: The results showed that 2% of the pregnant women were at risk of T. gondii transmission during the gestational period. These data seem to reflect the real situation of gestational toxoplasmosis in the northwestern region of the state of São Paulo.
- Published
- 2011
- Full Text
- View/download PDF
34. Seropositivity rates for toxoplasmosis, rubella, syphilis, cytomegalovirus, hepatitis and HIV among pregnant women receiving care at a public health service, São Paulo state, Brazil.
- Author
-
Gonçalves MA, Matos Cde C, Spegiorin LC, Oliani DC, Oliani AH, and Mattos LC
- Subjects
- Adolescent, Adult, Antibodies, Bacterial blood, Antibodies, Protozoan blood, Brazil epidemiology, Cytomegalovirus Infections diagnosis, Cytomegalovirus Infections epidemiology, Female, HIV Infections diagnosis, HIV Infections epidemiology, Hepatitis, Viral, Human diagnosis, Hepatitis, Viral, Human epidemiology, Humans, Immunoglobulin G blood, Immunoglobulin M blood, Immunologic Tests, Pregnancy, Pregnancy Complications, Infectious diagnosis, Rubella diagnosis, Rubella epidemiology, Seroepidemiologic Studies, Syphilis diagnosis, Toxoplasmosis diagnosis, Virus Diseases diagnosis, Young Adult, Pregnancy Complications, Infectious epidemiology, Syphilis epidemiology, Toxoplasmosis epidemiology, Virus Diseases epidemiology
- Abstract
Infectious and parasitic diseases affecting women during their reproductive age may result in vertical transmission. The aim of this study was to determine the seroprevalence for TORSCH among pregnant women receiving care at a university hospital. Records of 574 pregnant women who received medical attention from January 2006 to December 2007 were assessed. The mean age was 27.2 ± 6.5 years ranging from 13 to 44. The results of the immunodiagnostic tests were: 62.0% (345/556) for IgG and 3.4% (19/556) for IgM anti-T. gondii; 93.1% (433/465) for IgG and 0.6% (3/465) for IgM anti-rubella; 0.9% (5/561) for VDRL; 1.8% (10/554) for HBsAg; 0.7% (4/545) for anti-HCV and 2.1% (11/531) for HIV. In conclusion, the results of immunodiagnostic tests for the TORSCH panel among pregnant women attending a perinatal service of a university hospital are in agreement with those reported by previous studies and by governmental sources.
- Published
- 2010
35. Prevalence of anticardiolipin antibodies in pregnancies with history of repeated miscarriages.
- Author
-
Spegiorin LC, Galão EA, Bagarelli LB, Oliani AH, and de Godoy JM
- Abstract
Aim: This study aimed at evaluating the prevalence of high anticardiolipin antibody and lupus anticoagulant levels in women with histories of at least two miscarriages., Methods: The presence of high anticardiolipin antibody and lupus anticoagulant levels were evaluated in 52 patients with ages between 17 and 41 (mean 26.7 years old) who had histories of repeated miscarriages., Results: High anticardiolipin antibody levels were identified in 55.77% and lupus anticoagulant levels in 2% of the individuals., Conclusion: In conclusion, women who suffer from repeated miscarriages present with a high prevalence of anticardiolipin antibodies. Further studies on this issue are crucial as these antibodies are associated with hypercoagulability and thus increased the risk of thromboembolic events.
- Published
- 2010
- Full Text
- View/download PDF
36. Identification of B- and T-cell epitopes within the MTP40 protein of Mycobacterium tuberculosis and their correlation with the disease course.
- Author
-
Falla JC, Parra CA, Mendoza M, Franco LC, Guzmán F, Forero J, Orozco O, and Patarroyo ME
- Subjects
- Amino Acid Sequence, Antibodies, Bacterial immunology, Antibody Specificity, Antigens, Bacterial chemistry, Epitopes, Humans, Lymphocyte Activation, Molecular Sequence Data, Peptides chemistry, Tuberculosis immunology, Antigens, Bacterial immunology, B-Lymphocytes immunology, Bacterial Proteins, Mycobacterium tuberculosis immunology, T-Lymphocytes immunology, Type C Phospholipases
- Abstract
Synthetic peptides derived from the amino acid sequence of MTP40, a recently characterized Mycobacterium tuberculosis protein, were tested by two different immunological assays in 91 individuals. For the purposes of this study, the population was distributed in four groups: active tuberculosis (TBC) patients with elevated bacillus loads (BK+), active TBC patients with low bacillus loads (BK-), healthy individuals living in the same household with tuberculous patients (HH), and normal individuals, who had presumably never been in contact with the bacilli (control). We found that T cells of individuals belonging to the HH group showed the highest and most frequent recognition of these peptides in a T-cell proliferation assay, while their antibodies showed the lowest recognition of these peptides when tested by enzyme-linked immunosorbent assay. In contrast, TBC patients revealed an inverse pattern of immune response. Interestingly, one of these peptides (P7) was recognized by T cells of 64% of the HH individuals and by 4.5% of normal donors. Another peptide (P4) was recognized by 55% of sera from BK+ patients and by 5.5% of normal donors. The results presented here indicate the existence of T- and B-cell epitopes within the MTP40 protein. Given the particular recognition pattern of this protein, added to the fact that it appears to be a species-specific antigen of M. tuberculosis, a detailed study of the immune response to it may be useful in the design of more accurate diagnostic tests and an improved vaccine against human TBC.
- Published
- 1991
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.