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Identification of B- and T-cell epitopes within the MTP40 protein of Mycobacterium tuberculosis and their correlation with the disease course.
- Source :
-
Infection and immunity [Infect Immun] 1991 Jul; Vol. 59 (7), pp. 2265-73. - Publication Year :
- 1991
-
Abstract
- Synthetic peptides derived from the amino acid sequence of MTP40, a recently characterized Mycobacterium tuberculosis protein, were tested by two different immunological assays in 91 individuals. For the purposes of this study, the population was distributed in four groups: active tuberculosis (TBC) patients with elevated bacillus loads (BK+), active TBC patients with low bacillus loads (BK-), healthy individuals living in the same household with tuberculous patients (HH), and normal individuals, who had presumably never been in contact with the bacilli (control). We found that T cells of individuals belonging to the HH group showed the highest and most frequent recognition of these peptides in a T-cell proliferation assay, while their antibodies showed the lowest recognition of these peptides when tested by enzyme-linked immunosorbent assay. In contrast, TBC patients revealed an inverse pattern of immune response. Interestingly, one of these peptides (P7) was recognized by T cells of 64% of the HH individuals and by 4.5% of normal donors. Another peptide (P4) was recognized by 55% of sera from BK+ patients and by 5.5% of normal donors. The results presented here indicate the existence of T- and B-cell epitopes within the MTP40 protein. Given the particular recognition pattern of this protein, added to the fact that it appears to be a species-specific antigen of M. tuberculosis, a detailed study of the immune response to it may be useful in the design of more accurate diagnostic tests and an improved vaccine against human TBC.
- Subjects :
- Amino Acid Sequence
Antibodies, Bacterial immunology
Antibody Specificity
Antigens, Bacterial chemistry
Epitopes
Humans
Lymphocyte Activation
Molecular Sequence Data
Peptides chemistry
Tuberculosis immunology
Antigens, Bacterial immunology
B-Lymphocytes immunology
Bacterial Proteins
Mycobacterium tuberculosis immunology
T-Lymphocytes immunology
Type C Phospholipases
Subjects
Details
- Language :
- English
- ISSN :
- 0019-9567
- Volume :
- 59
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Infection and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 1711013
- Full Text :
- https://doi.org/10.1128/iai.59.7.2265-2273.1991