9 results on '"Fourniol, Lisa"'
Search Results
2. Method for tick-borne encephalitis virus detection in raw milk products
- Author
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Hennechart-Collette, Catherine, Gonzalez, Gaëlle, Fourniol, Lisa, Fraisse, Audrey, Beck, Cécile, Moutailler, Sara, Bournez, Laure, Dheilly, Nolwenn M., Lacour, Sandrine A., Lecollinet, Sylvie, Martin-Latil, Sandra, and Perelle, Sylvie
- Published
- 2022
- Full Text
- View/download PDF
3. Evaluation of a Proteinase K-Based Extraction Method to Detect Hepatitis A Virus, Hepatitis E Virus and Norovirus in Artificially Contaminated Dairy Products
- Author
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Hennechart-Collette, Catherine, primary, Fourniol, Lisa, additional, Fraisse, Audrey, additional, Martin-Latil, Sandra, additional, and Perelle, Sylvie, additional
- Published
- 2023
- Full Text
- View/download PDF
4. A laboratory-based study to explore the use of honey-impregnated cards to detect chikungunya virus in mosquito saliva
- Author
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Fourniol, Lisa, Madec, Yoann, Mousson, Laurence, Vazeille, Marie, Failloux, Anna-Bella, Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP), Epidémiologie des Maladies Emergentes - Emerging Diseases Epidemiology, Pasteur-Cnam Risques infectieux et émergents (PACRI), Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), This study was supported by the European Union’s Horizon 2020 research and innovation program under EVAg grant agreement no. 653316 and the French Government’s Investissement d’Avenir program, Laboratoire d’Excellence 'Integrative Biology of Emerging Infectious Diseases' (grant n°ANR-10-LABX-62-IBEID)., We thank Malika Hocine and Jocelyne Alexandre for helping in rearing mosquitoes., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 653316,H2020,H2020-INFRAIA-2014-2015,EVAg(2015), Institut Pasteur [Paris], and Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM)
- Subjects
RNA viruses ,Physiology ,viruses ,[SDV]Life Sciences [q-bio] ,Disease Vectors ,Pathology and Laboratory Medicine ,Mosquitoes ,Medical Conditions ,Filter Paper ,Animal Products ,Aedes ,Medicine and Health Sciences ,Chikungunya Virus ,Reverse Transcriptase Polymerase Chain Reaction ,Eukaryota ,virus diseases ,Agriculture ,Honey ,Body Fluids ,Insects ,Laboratory Equipment ,Infectious Diseases ,Blood ,Medical Microbiology ,Viral Pathogens ,Viruses ,Engineering and Technology ,RNA, Viral ,Medicine ,Pathogens ,Anatomy ,Salivation ,Research Article ,Paper ,Arthropoda ,Alphaviruses ,Science ,education ,Equipment ,Aedes Aegypti ,Microbiology ,Specimen Handling ,Togaviruses ,parasitic diseases ,Animals ,Saliva ,Microbial Pathogens ,Nutrition ,fungi ,Organisms ,Biology and Life Sciences ,Invertebrates ,Insect Vectors ,Diet ,Species Interactions ,Food ,Physiological Processes ,Zoology ,Entomology - Abstract
International audience; Mosquito control is implemented when arboviruses are detected in patients or in field-collected mosquitoes. However, mass screening of mosquitoes is usually laborious and expensive, requiring specialized expertise and equipment. Detection of virus in mosquito saliva using honey-impregnated filter papers seems to be a promising method as it is non-destructive and allows monitoring the viral excretion dynamics over time from the same mosquito. Here we test the use of filter papers to detect chikungunya virus in mosquito saliva in laboratory conditions, before proposing this method in large-scale mosquito surveillance programs. We found that 0.9 cm 2 cards impregnated with a 50% honey solution could replace the forced salivation technique as they offered a viral RNA detection until 7 days after oral infection of Aedes aegypti and Aedes albopictus mosquitoes with CHIKV.
- Published
- 2021
5. Experimental Ixodes ricinus-Sheep Cycle of Anaplasma phagocytophilum NV2Os Propagated in Tick Cell Cultures
- Author
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Almazán, Consuelo, Fourniol, Lisa, Rouxel, Clotilde, Alberdi, Pilar, Gandoin, Christelle, Lagrée, Anne-Claire, Boulouis, Henri-Jean, de la Fuente, José, Bonnet, Sarah, Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), École nationale vétérinaire d'Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Instituto de Investigación en Recursos Cinegéticos (IREC), Oklahoma State University [Stillwater], SATTIdF Innov, ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), École nationale vétérinaire - Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, and Agence Nationale de la Recherche (France)
- Subjects
sheep ,Anaplasma ,[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health ,NV2Os ,Garrapatas ,animal diseases ,fungi ,Ixodes ricinus ,bacterial infections and mycoses ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Ovejas ,parasitic diseases ,tick cell cultures ,Veterinary Science ,Original Research ,Anaplasma phagocytophilum - Abstract
The causative agent of tick-borne fever and human granulocytic anaplasmosis, Anaplasma phagocytophilum, is transmitted by Ixodes ricinus, and is currently considered an emerging disease throughout Europe. In this study, we established a model of A. phagocytophilum sheep infection and I. ricinus transmission using the European Norway variant 2 ovine strain (NV2Os) propagated in both IDE8 and ISE6 tick cells. Two sheep were inoculated with IDE8 tick cells infected with NV2Os. Both sheep developed A. phagocytophilum infection as determined by qPCR and PCR, the presence of fever 4 days post inoculation (dpi), the observation of morulae in granulocytes at 6 dpi, and the detection of A. phagocytophilum antibodies at 14 dpi. A. phagocytophilum was detected by PCR in skin, lung, small intestine, liver, spleen, uterus, bone marrow, and mesenteric lymph node from necropsies performed at 14 and 15 dpi. One sheep was infested during the acute phase of infection with I. ricinus nymphs from a pathogen-free colony. After molting, A. phagocytophilum transstadial transmission in ticks was validated with qPCR positive bacterial detection in 80% of salivary glands and 90% of midguts from female adults. Infected sheep blood collected at 14 dpi was demonstrated to be able to infect ISE6 tick cells, thus enabling the infection of two additional naive sheep, which then went on to develop similar clinical signs to the sheep infected previously. One of the sheep remained persistently infected until 115 dpi when it was euthanized, and transmitted bacteria to 70 and 2.7% of nymphs engorged as larvae during the acute and persistent infection stages, respectively. We then demonstrated that these infected nymphs were able to transmit the bacteria to one of two other naive infested sheep. As expected, when I. ricinus females were engorged during the acute phase of infection, no A. phagocytophilum transovarial transmission was detected. The development of this new experimental model will facilitate future research on this tick-borne bacterium of increasing importance, and enable the evaluation of any new tick/transmission control strategies., This study received funding from the French Government's Investissement d'Avenir program, Laboratoire d'Excellence Integrative Biology of Emerging Infectious Diseases (grant n°ANR-10-LABX-62-IBEID) and from the SATTIdF Innov.
- Published
- 2020
6. Multiple Antigenic Peptide-Based Vaccines Targeting Ixodes ricinus Neuropeptides Induce a Specific Antibody Response but Do Not Impact Tick Infestation
- Author
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Almazán, Consuelo, primary, Šimo, Ladislav, additional, Fourniol, Lisa, additional, Rakotobe, Sabine, additional, Borneres, Jérémie, additional, Cote, Martine, additional, Peltier, Sandy, additional, Mayé, Jennifer, additional, Versillé, Nicolas, additional, Richardson, Jennifer, additional, and Bonnet, Sarah I., additional
- Published
- 2020
- Full Text
- View/download PDF
7. Failed Disruption of Tick Feeding, Viability, and Molting after Immunization of Mice and Sheep with Recombinant Ixodes ricinus Salivary Proteins IrSPI and IrLip1
- Author
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Almazán, Consuelo, primary, Fourniol, Lisa, additional, Rakotobe, Sabine, additional, Šimo, Ladislav, additional, Bornères, Jérémie, additional, Cote, Martine, additional, Peltier, Sandy, additional, Maye, Jennifer, additional, Versillé, Nicolas, additional, Richardson, Jennifer, additional, and Bonnet, Sarah I., additional
- Published
- 2020
- Full Text
- View/download PDF
8. A laboratory-based study to explore the use of honey-impregnated cards to detect chikungunya virus in mosquito saliva.
- Author
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Fourniol L, Madec Y, Mousson L, Vazeille M, and Failloux AB
- Subjects
- Animals, Chikungunya virus isolation & purification, Honey, Paper, RNA, Viral genetics, RNA, Viral metabolism, Reverse Transcriptase Polymerase Chain Reaction instrumentation, Saliva virology, Specimen Handling instrumentation, Specimen Handling methods, Aedes virology, Chikungunya virus genetics, Reverse Transcriptase Polymerase Chain Reaction methods
- Abstract
Mosquito control is implemented when arboviruses are detected in patients or in field-collected mosquitoes. However, mass screening of mosquitoes is usually laborious and expensive, requiring specialized expertise and equipment. Detection of virus in mosquito saliva using honey-impregnated filter papers seems to be a promising method as it is non-destructive and allows monitoring the viral excretion dynamics over time from the same mosquito. Here we test the use of filter papers to detect chikungunya virus in mosquito saliva in laboratory conditions, before proposing this method in large-scale mosquito surveillance programs. We found that 0.9 cm2 cards impregnated with a 50% honey solution could replace the forced salivation technique as they offered a viral RNA detection until 7 days after oral infection of Aedes aegypti and Aedes albopictus mosquitoes with CHIKV., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2021
- Full Text
- View/download PDF
9. Experimental Ixodes ricinus -Sheep Cycle of Anaplasma phagocytophilum NV2Os Propagated in Tick Cell Cultures.
- Author
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Almazán C, Fourniol L, Rouxel C, Alberdi P, Gandoin C, Lagrée AC, Boulouis HJ, de la Fuente J, and Bonnet SI
- Abstract
The causative agent of tick-borne fever and human granulocytic anaplasmosis, Anaplasma phagocytophilum , is transmitted by Ixodes ricinus , and is currently considered an emerging disease throughout Europe. In this study, we established a model of A. phagocytophilum sheep infection and I. ricinus transmission using the European Norway variant 2 ovine strain (NV2Os) propagated in both IDE8 and ISE6 tick cells. Two sheep were inoculated with IDE8 tick cells infected with NV2Os. Both sheep developed A. phagocytophilum infection as determined by qPCR and PCR, the presence of fever 4 days post inoculation (dpi), the observation of morulae in granulocytes at 6 dpi, and the detection of A. phagocytophilum antibodies at 14 dpi. A. phagocytophilum was detected by PCR in skin, lung, small intestine, liver, spleen, uterus, bone marrow, and mesenteric lymph node from necropsies performed at 14 and 15 dpi. One sheep was infested during the acute phase of infection with I. ricinus nymphs from a pathogen-free colony. After molting, A. phagocytophilum transstadial transmission in ticks was validated with qPCR positive bacterial detection in 80% of salivary glands and 90% of midguts from female adults. Infected sheep blood collected at 14 dpi was demonstrated to be able to infect ISE6 tick cells, thus enabling the infection of two additional naive sheep, which then went on to develop similar clinical signs to the sheep infected previously. One of the sheep remained persistently infected until 115 dpi when it was euthanized, and transmitted bacteria to 70 and 2.7% of nymphs engorged as larvae during the acute and persistent infection stages, respectively. We then demonstrated that these infected nymphs were able to transmit the bacteria to one of two other naive infested sheep. As expected, when I. ricinus females were engorged during the acute phase of infection, no A. phagocytophilum transovarial transmission was detected. The development of this new experimental model will facilitate future research on this tick-borne bacterium of increasing importance, and enable the evaluation of any new tick/transmission control strategies., (Copyright © 2020 Almazán, Fourniol, Rouxel, Alberdi, Gandoin, Lagrée, Boulouis, de la Fuente and Bonnet.)
- Published
- 2020
- Full Text
- View/download PDF
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