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A laboratory-based study to explore the use of honey-impregnated cards to detect chikungunya virus in mosquito saliva
- Source :
- PLoS ONE, PLoS ONE, 2021, 16 (4), pp.e0249471. ⟨10.1371/journal.pone.0249471⟩, PLoS ONE, Public Library of Science, 2021, 16 (4), pp.e0249471. ⟨10.1371/journal.pone.0249471⟩, PLoS ONE, Vol 16, Iss 4, p e0249471 (2021)
- Publication Year :
- 2021
- Publisher :
- HAL CCSD, 2021.
-
Abstract
- International audience; Mosquito control is implemented when arboviruses are detected in patients or in field-collected mosquitoes. However, mass screening of mosquitoes is usually laborious and expensive, requiring specialized expertise and equipment. Detection of virus in mosquito saliva using honey-impregnated filter papers seems to be a promising method as it is non-destructive and allows monitoring the viral excretion dynamics over time from the same mosquito. Here we test the use of filter papers to detect chikungunya virus in mosquito saliva in laboratory conditions, before proposing this method in large-scale mosquito surveillance programs. We found that 0.9 cm 2 cards impregnated with a 50% honey solution could replace the forced salivation technique as they offered a viral RNA detection until 7 days after oral infection of Aedes aegypti and Aedes albopictus mosquitoes with CHIKV.
- Subjects :
- RNA viruses
Physiology
viruses
[SDV]Life Sciences [q-bio]
Disease Vectors
Pathology and Laboratory Medicine
Mosquitoes
Medical Conditions
Filter Paper
Animal Products
Aedes
Medicine and Health Sciences
Chikungunya Virus
Reverse Transcriptase Polymerase Chain Reaction
Eukaryota
virus diseases
Agriculture
Honey
Body Fluids
Insects
Laboratory Equipment
Infectious Diseases
Blood
Medical Microbiology
Viral Pathogens
Viruses
Engineering and Technology
RNA, Viral
Medicine
Pathogens
Anatomy
Salivation
Research Article
Paper
Arthropoda
Alphaviruses
Science
education
Equipment
Aedes Aegypti
Microbiology
Specimen Handling
Togaviruses
parasitic diseases
Animals
Saliva
Microbial Pathogens
Nutrition
fungi
Organisms
Biology and Life Sciences
Invertebrates
Insect Vectors
Diet
Species Interactions
Food
Physiological Processes
Zoology
Entomology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, PLoS ONE, 2021, 16 (4), pp.e0249471. ⟨10.1371/journal.pone.0249471⟩, PLoS ONE, Public Library of Science, 2021, 16 (4), pp.e0249471. ⟨10.1371/journal.pone.0249471⟩, PLoS ONE, Vol 16, Iss 4, p e0249471 (2021)
- Accession number :
- edsair.pmid.dedup....49d82395f9773830f0ecd75683b9fdba