1. CHARACTERISATION AND BIOLOGICAL ACTIVITIES OF A FOREST Streptomyces Too2 ISOLATED FROM WESTERN ALGERIA PRODUCING BLANCHAQUINONE.
- Author
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Khodja, Fatima Idris, Hamini-Kadar, Nisserine, AmineGacem, Mohamed, and Kihal, Mebrouk
- Abstract
Exploring natural and uncharted ecosystems can yield novel biocontrol agents with potential benefits in both pharmaceuticals and agriculture. Microorganisms, being economically sustainable and biologically valuable, are a valuable source for the production of biocontrol compounds. The objective of this study is to assess the bioactive capabilities, specifically the antimicrobial and antioxidant properties, of Streptomyces sp. T
oo2 , which was isolated, for the first time, from the "Tifrit forest" in western Algeria. This study involved collecting 42 soil samples from Tifrit forest located in Western Algeria to evaluate their potential for producing bioactive substances. The Too2 isolate, which demonstrated the highest level ofbioactivity, was identified using various techniques such as mmphological, physiological, biochemical, and molecular characterization. The bioactivity of the metabolites produced by this isolate was then assessed through in vitro evaluation. Finally, the crude extract was analyzed using liquid chromatography and mass spectrometry (LC-MS). The potent antimicrobial activity and significant antioxidant scavenging properties of the ethyl acetate extract ofToo2 were evident. TheHPLC-MS analysis identified Blanchaquinone, a major compound in the extract, which may be responsible for its antimicrobial and antioxidant properties. The taxonomic position of Too2 was determined to be similar to Streptomyces galilaus through the analysis of its 16S rRNA sequence. The strain Too2 seems to hold great potential for producing new molecules that could be useful in the field of medicine. [ABSTRACT FROM AUTHOR]- Published
- 2023