2,621 results on '"Foodborne Diseases prevention & control"'
Search Results
2. Impact of non-pharmaceutical interventions during the COVID-19 pandemic on pathogens transmitted via food in the Netherlands.
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Pijnacker R, Mughini-Gras L, Verhoef L, van den Beld M, Franz E, and Friesema I
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- Humans, Netherlands epidemiology, Incidence, SARS-CoV-2, Gastroenteritis epidemiology, Gastroenteritis prevention & control, Gastroenteritis virology, COVID-19 epidemiology, COVID-19 prevention & control, COVID-19 transmission, Foodborne Diseases epidemiology, Foodborne Diseases prevention & control, Foodborne Diseases virology
- Abstract
The COVID-19 pandemic impacted the transmission of many pathogens. The aim was to determine the effect of non-pharmaceutical interventions on the incidence of diseases transmitted via food. Weekly incidence rates for nine foodborne pathogens were collected from national surveillance registries. Weekly pathogen incidence during lockdown weeks of 2020 and 2021 were compared with corresponding weeks in 2015-2019. The same analyses were performed to determine the effect of self-defined expected impact levels of measures (low, intermediate and high). Eight out of 9 diseases showed a significant decrease in case number in 2020, except for listeriosis, which remained unchanged. The largest decrease was observed for rotavirus gastronteritis A (-81%), norovirus gastroenteritis (-78%), hepatitis A (-75%) and shigellosis (-72). In 2021, lower case numbers were observed for 6 out of 9 diseases compared with 2015-2019, with the largest decrease for shigellosis (-5/%) and hepatitis E (-47%). No significant change was observed for listeriosis, STEC infection and rotavirus gastroenteritis. Overall, measures with increased expected impact level did not result in a larger decrease in number of cases, except for Campylobacter, and norovirus and rotavirus gastroenteritis. Disease transmitted via food significantly decreased during the COVID-19 pandemic, with a more pronounced effect during 2020 than 2021.
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- 2024
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3. Empowering consumers to purchase safe ready-to-eat chicken from street restaurants in Ouagadougou, Burkina Faso: impact of a multi-media behavior change campaign.
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Madjdian DS, van Asseldonk M, Talsma EF, Dione M, Ilboudo G, Roesel K, Grace D, Knight-Jones TJD, and de Vet E
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- Burkina Faso, Humans, Animals, Adult, Male, Female, Health Knowledge, Attitudes, Practice, Middle Aged, Young Adult, Social Media, Adolescent, Foodborne Diseases prevention & control, Chickens, Restaurants, Consumer Behavior, Food Safety
- Abstract
Foodborne disease resulting from food sold at urban informal markets is a major public health challenge in Sub-Saharan Africa. This study assessed the impact of an innovative nine-months multi-media campaign engaging a key influencer, aimed at empowering consumers to choose safer ready-to-eat chicken meat at informal street restaurants in Ouagadougou, Burkina Faso. A two-wave panel study assessed associations between recall of TV, radio, billboard, and social media advertisements, and self-reported behavior regarding purchasing and consumption of ready-to-eat chicken at outlets, consumer intentions, knowledge, attitudes, norms, and agency. A panel of randomly selected adult consumers (n = 852) were interviewed pre- and post-campaign, 12 months apart. 60% recalled at least one of the campaign channels when prompted. Mixed-effects models showed associations between prompted recall and feeling better informed about food safety (aOR 1.449) and increased knowledge (0.132 unit increase on total score). Social media recall was associated with higher perceived access to information (aOR 1.449) and knowledge. Billboard recall increased odds of higher perceived health benefits of paying attention to food safety behaviors when purchasing chicken (aOR 2.046). TV ad recall was associated with a 0.159 unit decrease in the gap between consumers' intentions and behavior. An engaging consumer food safety multimedia campaign that engages key influencers improved food safety awareness and knowledge, ultimately supporting consumers to choose safer chicken at markets., (© 2024. The Author(s).)
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- 2024
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4. Application of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry for Identification of Foodborne Pathogens: Current Developments and Future Trends.
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Zeng X, Wang Y, Shen X, Wang H, and Xu ZL
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- Food Contamination analysis, Humans, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods, Food Microbiology methods, Bacteria isolation & purification, Bacteria classification, Bacteria chemistry, Bacteria genetics, Foodborne Diseases microbiology, Foodborne Diseases prevention & control
- Abstract
Foodborne pathogens have gained sustained public attention, exerted significant pressure on food manufacturers, and posed serious health risks to human. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been employed for quick and accurate identification of microorganisms in the prevention of foodborne epidemics in recent years. Herein, we first summarize the principle of MALDI and its workflow for foodborne pathogens. Subsequently, we review the recent progress and applications of MALDI-TOF MS in foodborne pathogen determination. Additionally, we outline the expanded utilization of MALDI-based techniques for the identification of closely related species. We also assess the current gaps and propose possible solutions to address the existing challenges. MALDI-TOF MS is a promising biotool for rapid and accurate identification of foodborne microbes at the species and genus level in food samples. Database expansion and direct quantification of spoilage microbes are two promising areas for future progress in MALDI-TOF MS applications.
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- 2024
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5. Gut Lactococcus garvieae promotes protective immunity to foodborne Clostridium perfringens infection.
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Wang X-Y, Meng F-H, Zhang M-Y, Li F-X, Lei Y-X, Ma Z-G, Li J-Q, Lou Y-N, Chu Y-F, Ma K, and Yu S-X
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- Animals, Mice, Sheep, Foodborne Diseases microbiology, Foodborne Diseases prevention & control, Female, Disease Models, Animal, Dysbiosis microbiology, Dysbiosis prevention & control, Dysbiosis immunology, Clostridium perfringens immunology, Clostridium perfringens physiology, Clostridium Infections immunology, Clostridium Infections prevention & control, Clostridium Infections microbiology, Clostridium Infections veterinary, Gastrointestinal Microbiome, Lactococcus, Probiotics administration & dosage, Intestinal Mucosa microbiology, Intestinal Mucosa immunology
- Abstract
The gut microbiota, a pivotal component of the intestinal mucosal barrier, is critical for host resistance to enteric pathogen infection. Here, we report a novel function of the potentially probiotic Lactococcus garvieae strain LG1 ( L. garvieae strain LG1) in maintaining intestinal mucosal barrier integrity and protecting against foodborne Clostridium perfringens ( C. perfringens ) infection. L. garvieae was isolated from the intestinal contents of Chinese Mongolian sheep (MS) and exhibited potential probiotic properties. In a C. perfringens enterocolitis model, L. garvieae- pretreated mice were less susceptible to C. perfringens infection compared with Phosphate buffered solution (PBS) - pretreated mice, which manifested as higher survival rates, lower pathogen loads, less weight loss, mild clinical symptoms and intestinal damage, and minor inflammation. Further mechanistic analysis showed that L. garvieae could ameliorate the disruption of intestinal permeability and maintain the integrity of the intestinal mucosal barrier by promoting the expression of tight junction proteins and mucoproteins. Moreover, L. garvieae was also able to facilitate antimicrobial peptide expression and ameliorate dysbiosis of the gut microbiota caused by C. perfringens . Together, these findings highlight the prospect of immunomodulatory potentially probiotic L. garvieae and might offer valuable strategies for prophylaxis and/or treatment of pathogenic C. perfringens mucosal infection., Importance: C. perfringens necrotic enteritis leads to losses of about US $2 billion to the poultry industry worldwide every year. Worse, US Centers for Disease Control and Prevention (CDC) has estimated that C. perfringens causes nearly 1 million foodborne illnesses in the United States annually. Nowadays, the treatment recommendation is a combination of a broad-spectrum synergistic penicillin with clindamycin or a carbapenem, despite growing scientific concern over antibiotic resistance. The global understanding of the gut microbiome for C. perfringens infection may provide important insights into the intervention. L. garvieae originated from Mongolian sheep intestine, exhibited potentially probiotic properties, and was able to limit C. perfringens enterocolitis and pathogenic colonization. Importantly, we found that L. garvieae limits C. perfringens invasion via improving intestinal mucosal barrier function. Also, L. garvieae alleviates C. perfringens -induced gut microbiota dysbiosis. It allowed us to convince that utilization of probiotics to promote protective immunity against pathogens infection is of pivotal importance., Competing Interests: The authors declare no conflict of interest.
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- 2024
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6. Impact of COVID-19 on Chinese urban consumers' food safety knowledge and behavior - A comparative study between pre and post pandemic eras.
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Wang K, Cong L, Mirosa M, Bai L, Hou Y, and Bremer P
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- Humans, China epidemiology, Female, Male, Adult, Middle Aged, Young Adult, Surveys and Questionnaires, SARS-CoV-2, Consumer Behavior, Urban Population, Adolescent, Aged, Pandemics, COVID-19 epidemiology, COVID-19 prevention & control, Health Knowledge, Attitudes, Practice, Food Safety, Foodborne Diseases epidemiology, Foodborne Diseases prevention & control
- Abstract
The COVID-19 pandemic caused notable changes to the food-related habits of consumers worldwide due to their concerns about the risk of infection and the requirement to follow government mandates. To investigate the impact of the pandemic on Chinese consumers' food safety knowledge, food safety behaviors, and their most recent food poisoning experiences, we compared the results from an online survey (n = 583, Dec 2019) conducted before the pandemic was officially announced with an identical survey (n = 599, Aug 2023) conducted seven months after the Chinese government downgraded restrictions related to COVID-19. Post-pandemic there was a significant decrease in consumers' food safety knowledge and self-reported food safety behaviors and a significant increase in their self-reported experiences of food poisoning. Despite respondents stating that their food safety knowledge and behaviors had improved since the start of the pandemic, the data obtained from the two surveys and the respondents self-reporting of foodborne illness suggest that in fact their safety knowledge and behaviors had decreased. These findings highlight the need to reinforce food safety education and behaviors during times when the food system is disrupted, and consumers are focusing on what they perceive to be more immediate issues., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
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- 2024
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7. Research progress on detection of foodborne pathogens: The more rapid and accurate answer to food safety.
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Gao R, Liu X, Xiong Z, Wang G, and Ai L
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- Humans, CRISPR-Cas Systems, Food Contamination analysis, Foodborne Diseases microbiology, Foodborne Diseases prevention & control, Food Microbiology methods, Food Safety methods, Biosensing Techniques methods
- Abstract
In recent years, foodborne diseases have posed a serious threat to human health, and rapid detection of foodborne pathogens is particularly crucial for the prevention and control of such diseases. This article offers a detailed overview of the development of detection techniques for foodborne pathogens, transitioning from traditional microbiological culture methods to the current array of techniques, including immunological, molecular biological, and biosensor-based methods. It summarizes the technical principles, advantages, disadvantages, and research progress of these diverse methods. Furthermore, the article demonstrates that the combination of different methods enhances the efficiency and accuracy of pathogens detection. Specifically, the article focuses on the application and advantages of combining CRISPR/Cas systems with other detection methods in the detection of foodborne pathogens. CRISPR/Cas systems, with their high specificity, sensitivity, and ease of operation, show great potential in the field of foodborne pathogens detection. When integrated with other detection techniques such as immunological detection techniques, molecular biology detection techniques, and biosensors, the accuracy and efficiency of detection can be further improved. By fully utilizing these tools, early detection and control of foodborne diseases can be achieved, enhancing public health and preventing disease outbreaks. This article serves as a valuable reference for exploring more convenient, accurate, and sensitive field detection methods for foodborne pathogens, promoting the application of rapid detection techniques, and ensuring food safety and human health., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
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- 2024
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8. Home Delivery Meal Kits Online Food Safety-Related Information: A Perspective.
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Maneerat N, Byrd K, Almanza B, Behnke C, and Nelson D
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- Humans, Foodborne Diseases prevention & control, United States, Food Safety methods, Internet
- Abstract
The recent popularity of home delivery meal kits (HMK) has prompted concerns about its integrity and safety. On the basis of a food safety-related information evaluation of the common US-based HMK vendors' websites, this perspective highlights opportunities for improvement with the adequacy and accessibility of relevant information on HMK websites, an important resource for communicating food safety best practices to consumers. Identified gaps in information and inadequate delivery protocols potentially increase the risk of offering unsafe food to consumers. Suggestions for future research and recommendations for vendors, policymakers, and regulators to help protect consumers from potential foodborne illness risks are also discussed., (Copyright © 2024 Society for Nutrition Education and Behavior. Published by Elsevier Inc. All rights reserved.)
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- 2024
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9. Prioritization of vegetable-borne biological hazards in Argentina using a multicriteria decision analysis tool.
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Brusa V, Costa M, Oteiza JM, Galli L, Barril PA, Leotta GA, and Signorini M
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- Argentina epidemiology, Humans, Disease Outbreaks, Risk Assessment, Food Contamination analysis, Vegetables microbiology, Vegetables parasitology, Foodborne Diseases epidemiology, Foodborne Diseases prevention & control, Food Microbiology
- Abstract
Vegetables, especially those eaten raw, have been implicated in several foodborne disease outbreaks. Since multiple vegetable matrices and hazards are involved, risk managers have to prioritize those with the greatest impact on public health to design control strategies. In this study, a scientific-based risk ranking of foodborne pathogens transmitted by leafy green vegetables in Argentina was performed. The prioritization process included hazard identification, evaluation criteria identification and definition, criteria weighting, expert survey design and selection and call for experts, hazard score calculation, hazard ranking and variation coefficient, and result analysis. Regression tree analysis determined four risk clusters: high ( Cryptosporidum spp., Toxoplasma gondii , Norovirus), moderate ( Giardia spp., Listeria spp., Shigella sonnei ), low (Shiga toxin-producing Escherichia coli , Ascaris spp., Entamoeba histolytica , Salmonella spp., Rotavirus, Enterovirus) and very low ( Campylobacter jejuni , hepatitis A virus and Yersinia pseudotuberculosis ). Diseases caused by Norovirus, Cryptosporidium spp. and T. gondii do not require mandatory notification. Neither viruses nor parasites are included as microbiological criteria for foodstuff. The lack of outbreak studies did not allow to accurately identify vegetables as a source of Norovirus disease. Information on listeriosis cases or outbreaks due to vegetable consumption was not available. Shigella spp. was the main responsible for bacterial diarrhea, but it has not been epidemiologically associated with vegetable consumption. The quality of the available information for all hazards studied was very low and low. The implementation of good practice guidelines throughout the entire vegetable production chain could prevent the presence of the identified hazards. The current study allowed the identification of vacancy areas and could help reinforce the need for performing epidemiological studies on foodborne diseases potentially associated with vegetable consumption in Argentina., Competing Interests: DECLARATION OF CONFLICTING INTERESTSThe authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
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- 2024
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10. Antibacterial, bacteriolytic, and antibiofilm activities of the essential oil of temu giring ( Curcuma heyneana Val.) against foodborne pathogens.
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Septama AW, Tasfiyati AN, Rahmi EP, Jantan I, Dewi RT, and Jaisi A
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- Bacillus cereus drug effects, Food Microbiology, Tetracycline pharmacology, Salmonella typhi drug effects, Shigella sonnei drug effects, Plant Oils pharmacology, Oils, Volatile pharmacology, Oils, Volatile chemistry, Anti-Bacterial Agents pharmacology, Biofilms drug effects, Microbial Sensitivity Tests, Curcuma chemistry, Foodborne Diseases prevention & control, Escherichia coli drug effects
- Abstract
Foodborne pathogens may cause foodborne illness, which is among the major health problems worldwide. Since the therapeutic options for the treatment of the disease are becoming limited as a result of antibacterial resistance, there is an increasing interest to search for new alternatives of antibacterial. Bioactive essential oils from Curcuma sp become potential sources of novel antibacterial substances. The antibacterial activity of Curcuma heyneana essential oil (CHEO) was evaluated against Escherichia coli, Salmonella typhi, Shigella sonnei , and Bacillus cereus . The principal constituents of CHEO are ar-turmerone, β-turmerone, α-zingiberene, α-terpinolene, 1,8-cineole, and camphor. CHEO exhibited the strongest antibacterial activity against E. coli with a MIC of 3.9 µg/mL, which is comparable to that of tetracycline. The combination of CHEO (0.97 µg/mL) and tetracycline (0.48 µg/mL) produced a synergistic effect with a FICI of 0.37. Time-kill assay confirmed that CHEO enhanced the activity of tetracycline. The mixture disrupted membrane permeability of E. coli and induced cell death. CHEO at MIC of 3.9 and 6.8 µg/mL significantly reduced the formation of biofilm in E. coli . The findings suggest that CHEO has the potential to be an alternative source of antibacterial agents against foodborne pathogens, particularly E. coli ., Competing Interests: DECLARATION OF CONFLICTING INTERESTSThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
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- 2024
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11. Ingesting Risk - The FDA and New Food Ingredients.
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Cohen PA and Broad Leib EM
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- Humans, Government Regulation, Risk, United States, Food Safety, Food Ingredients analysis, Food Ingredients poisoning, Food Ingredients standards, United States Food and Drug Administration standards, Meat Substitutes analysis, Meat Substitutes poisoning, Meat Substitutes standards, Foodborne Diseases etiology, Foodborne Diseases prevention & control, Chemical and Drug Induced Liver Injury etiology, Chemical and Drug Induced Liver Injury prevention & control
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- 2024
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12. Evaluation of inhibitory activity of essential oils and natural extracts on foodborne viruses.
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Amri M, Jubinville É, Goulet-Beaulieu V, Fliss I, and Jean J
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- Animals, Vaccinium macrocarpon chemistry, Thymus Plant chemistry, Mice, Grape Seed Extract pharmacology, Rosmarinus chemistry, Food Microbiology, Foodborne Diseases prevention & control, Foodborne Diseases virology, Tea chemistry, Oils, Volatile pharmacology, Plant Extracts pharmacology, Antiviral Agents pharmacology, Herpesvirus 1, Human drug effects, Norovirus drug effects, Hepatitis A virus drug effects
- Abstract
Aims: Enteric viruses are recognized as a major concern in health care and in the food sector in Canada. Novel clean-label strategies for controlling enteric viruses are sought in the food industry. In this study, we examined the antiviral potential of plant extracts and essential oils on murine norovirus 1 (MNV-1), hepatitis A virus (HAV), and herpes simplex virus 1 (HSV-1)., Methods and Results: Inactivation of the viruses by grape seed, blueberry, green tea, and cranberry extracts and by rosemary and thyme essential oils was measured using plaque formation assay. Concentrations ranging from 50 to 200 000 ppm with a contact time of 90 min were tested. Grape seed extract at 10 000 ppm was the most effective (P < 0.05) at reducing MNV-1 and HAV infectious titers, respectively, by 2.85 ± 0.44 log10 and 1.94 ± 0.17 log10. HSV-1 titer was reduced by 3.81 ± 0.40 log10 at 1000 ppm grape seed extract., Conclusions: Among the plant products tested, grape seed extract was found the most effective at reducing the infectious titers of MNV-1, HAV, and HSV., (© The Author(s) 2024. Published by Oxford University Press on behalf of Applied Microbiology International.)
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- 2024
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13. Managing the microbiological safety of tilapia from farm to consumer.
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Ndraha N, Lin HY, Hsiao HI, and Lin HJ
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- Animals, Food Microbiology, Seafood microbiology, Food Safety methods, Humans, Foodborne Diseases prevention & control, Foodborne Diseases microbiology, Food Contamination prevention & control, Bacteria isolation & purification, Tilapia microbiology, Aquaculture methods
- Abstract
Tilapia stands out as one of the most extensively farmed and consumed fish species globally, valued for its ease of preparation and relative affordability. Although tilapia is a valuable protein source, it can also function as a vector for foodborne pathogens. This literature review reveals that tilapia could carry a variety of contamination with various foodborne pathogens, including Plesiomonas shigelloides, diarrheagenic Escherichia coli, Vibrio parahaemolyticus, Salmonella Weltevreden, Salmonella enterica, Shigella, Staphylococcus aureus, Campylobacter jejuni, Clostridium botulinum, and Listeria monocytogenes. Although guidelines from entities, such as the Global Seafood Alliance, Aquaculture Stewardship Council, and International Organization for Standardization, have been established to ensure the microbiological safety of tilapia, the unique challenges posed by pathogens in tilapia farming call for a more nuanced and targeted approach. Recognizing that contaminants could emerge at various stages of the tilapia supply chain, there is a crucial need for enhanced detection and monitoring of pathogens associated with this fish and its culturing environment. Additionally, it is essential to acknowledge the potential impact of climate change on the safety of tilapia, which may elevate the prevalence and contamination levels of pathogens in this fish. Proactive measures are essential to understand and mitigate the effects of climate change on tilapia production, ensuring the sustainability and safety of this seafood product for both present and future generations., (© 2024 Institute of Food Technologists®.)
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- 2024
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14. Risk factors affecting the food safety risk in food business operations for risk-based inspection: A systematic review.
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Ze Y, van Asselt ED, Focker M, and van der Fels-Klerx HJ
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- Risk Factors, Humans, Food Inspection methods, Food Inspection standards, Risk Assessment methods, Food Contamination prevention & control, Food Safety methods, Foodborne Diseases prevention & control
- Abstract
Foodborne illnesses result in a high disease burden worldwide, making food safety control of food business operations (FBOs) an urgent issue. With public agencies and FBOs facing challenges in monitoring the complex food supply chain with limited resources, scientific and objective insights into those factors that are related to food safety at FBOs are needed. These factors can be used as input for risk-based inspection. We conducted a systematic review to identify and analyze risk factors affecting the FBOs' food safety risk. We used a set of predefined search strings in Scopus and Web of Science to search for scientific manuscripts published in the English language between January 1 2003 and February 1 2023. The review identified 53 relevant studies and 43 risk factors. The presence of certified personnel turned out to be the most cited factor. Nearly half of the extracted factors had only been investigated in one study. Additional challenges were identified for developing a universal ready-to-use list of factors for the building of a risk-based inspection method, such as the limitation in the applicability of identified factors in different types of FBOs, and the variability in conclusions between publications for certain factors (e.g., FBO location and inspection history), stressing the need for additional research. Future studies should also prioritize standardizing definitions and measurements, particularly regarding compliance factors. In general, the current list of factors brought forward in our review lays the groundwork for building a transparent, objective, and risk-based method for food safety inspections of FBOs., (© 2024 The Author(s). Comprehensive Reviews in Food Science and Food Safety published by Wiley Periodicals LLC on behalf of Institute of Food Technologists.)
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- 2024
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15. Inactivation kinetics for surrogates of common foodborne pathogens during food residue drying.
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Shea E, Toniato J, and Simmons C
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- Kinetics, Food Handling methods, Temperature, Colony Count, Microbial, Food Preservation methods, Food Contamination prevention & control, Food Contamination analysis, Foodborne Diseases prevention & control, Foodborne Diseases microbiology, Listeria, Food Microbiology methods, Escherichia coli, Enterococcus faecium, Desiccation methods
- Abstract
Postconsumer household food residues can act as useful substrates for other industries, but transporting high-moisture material corresponds to high fuel use and associated greenhouse gas production. Drying food residues at the household level reduces transportation weight, increases stability, and preserves the nutritional quality of recovered material. Mitigating foodborne microbiological safety risks is crucial to encourage the development of novel methods to rapidly dry and stabilize food residues. The objective of this study was to improve the prediction of bacterial pathogen inactivation under various thermal and drying processes in a synthetic mixture of residual food material (RFM). The log reduction rate was measured for Escherichia coli, Enterococcus faecium, and Listeria innocua (surrogates of common foodborne pathogens) in RFM under different moisture contents (12% and 25% by fresh weight) and temperatures (50, 55, and 60°C). Inactivation data were used to determine D- and z-values and to fit a multiple regression model to predict log(D-values) in response to temperature and moisture content. Across conditions, D-values were measured to be 5.1-120, 4.6-123, and 32-545 min for E. coli, L. innocua, and E. faecium, respectively. Temperature sensitivities were significantly higher in lower moisture RFM for E. coli and L. innocua. Applying E. coli inactivation models during RFM at 55°C yielded inactivation rates that aligned with experimental values after 5 min (0.1 vs. 0-0.1 logs), 30 min (2.1 vs. 1.3-2.3 logs), and 90 min (7.2 vs. 7.1-8.9 logs). These results can inform the design of RFM drying and stabilization processes to promote pathogen inactivation and safety in downstream applications of dried material., (© 2024 The Author(s). Journal of Food Science published by Wiley Periodicals LLC on behalf of Institute of Food Technologists.)
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- 2024
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16. Foodborne bacterial pathogens: genome-based approaches for enduring and emerging threats in a complex and changing world.
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Mather AE, Gilmour MW, Reid SWJ, and French NP
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- Humans, Food Safety, Foodborne Diseases microbiology, Foodborne Diseases epidemiology, Foodborne Diseases prevention & control, Genome, Bacterial, Bacteria genetics, Bacteria classification, Food Microbiology
- Abstract
Foodborne illnesses pose a substantial health and economic burden, presenting challenges in prevention due to the diverse microbial hazards that can enter and spread within food systems. Various factors, including natural, political and commercial drivers, influence food production and distribution. The risks of foodborne illness will continue to evolve in step with these drivers and with changes to food systems. For example, climate impacts on water availability for agriculture, changes in food sustainability targets and evolving customer preferences can all have an impact on the ecology of foodborne pathogens and the agrifood niches that can carry microorganisms. Whole-genome and metagenome sequencing, combined with microbial surveillance schemes and insights from the food system, can provide authorities and businesses with transformative information to address risks and implement new food safety interventions across the food chain. In this Review, we describe how genome-based approaches have advanced our understanding of the evolution and spread of enduring bacterial foodborne hazards as well as their role in identifying emerging foodborne hazards. Furthermore, foodborne hazards exist in complex microbial communities across the entire food chain, and consideration of these co-existing organisms is essential to understanding the entire ecology supporting pathogen persistence and transmission in an evolving food system., (© 2024. Springer Nature Limited.)
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- 2024
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17. Revolutionizing food safety with electrochemical biosensors for rapid and portable pathogen detection.
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Ávila Oliveira B, Gomes RS, de Carvalho AM, Lima EMF, Pinto UM, and da Cunha LR
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- Humans, Bacteria isolation & purification, Bacteria classification, Bacteria genetics, Biosensing Techniques instrumentation, Biosensing Techniques methods, Food Safety methods, Electrochemical Techniques instrumentation, Electrochemical Techniques methods, Foodborne Diseases microbiology, Foodborne Diseases prevention & control, Food Microbiology methods, Food Microbiology instrumentation
- Abstract
Foodborne diseases remain a worldwide concern, despite the advances made in sanitation, pathogen surveillance and food safety management systems. The methods routinely applied for detecting pathogens in foods are time consuming, labor intensive and usually require trained and qualified individuals. The objective of this review was to highlight the use of biosensors, with a focus on the electrochemical devices, as promising alternatives for detecting foodborne pathogens. These biosensors present high speed for obtaining results, with the possibility of evaluating foods in real time, at low cost, ease of use, in addition to being compact and portable. These aspects are considered advantageous and suitable for use in food safety management systems. This work also shows some limitations for the application of biosensors, and we present perspectives with the development and use of nanomaterials., (© 2024. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
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- 2024
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18. Advances in the applications of Bacteriophages and phage products against food-contaminating bacteria.
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E S and Gummadi SN
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- Foodborne Diseases microbiology, Foodborne Diseases prevention & control, Food Safety, Food Contamination prevention & control, Humans, Food Preservation methods, Biological Control Agents, Bacteriophages physiology, Bacteriophages genetics, Food Microbiology methods, Bacteria virology
- Abstract
Food-contaminating bacteria pose a threat to food safety and the economy by causing foodborne illnesses and spoilage. Bacteriophages, a group of viruses that infect only bacteria, have the potential to control bacteria throughout the "farm-to-fork continuum". Phage application offers several advantages, including targeted action against specific bacterial strains and minimal impact on the natural microflora of food. This review covers multiple aspects of bacteriophages applications in the food industry, including their use as biocontrol and biopreservation agents to fight over 20 different genera of food-contaminating bacteria, reduce cross-contamination and the risk of foodborne diseases, and also to prolong shelf life and preserve freshness. The review also highlights the benefits of using bacteriophages in bioprocesses to selectively inhibit undesirable bacteria, such as substrate competitors and toxin producers, which is particularly valuable in complex microbial bioprocesses where physical or chemical methods become inadequate. Furthermore, the review briefly discusses other uses of bacteriophages in the food industry, such as sanitizing food processing environments and detecting specific bacteria in food products. The review also explores strategies to enhance the effectiveness of phages, such as employing multi-phage cocktails, encapsulated phages, phage products, and synergistic hurdle approaches by combining them with antimicrobials.
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- 2024
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19. Review: Impact of food safety on global trade.
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Tibebu A, Tamrat H, and Bahiru A
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- Foodborne Diseases prevention & control, Foodborne Diseases epidemiology, Humans, Food Supply, Animals, Food Safety, Commerce, Internationality
- Abstract
Food safety encompasses the supply and assurance of safe, high-quality food for consumers. It is a crucial aspect of food security, gaining greater global attention due to the increasing number of widespread foodborne incidents. International trade is expanding as countries increasingly rely on each other to secure a sufficient and diverse food supply. Beyond this, concerns about food safety have become more prevalent due to various factors. Therefore, this review aims to investigate the effects of food safety-associated risks on the international trade of food and related products. A total of 37 published studies retrieved using different search engines were included in this review. This review revealed that because of rapid population growth and rising food demand in developing nations, agricultural intensification is growing. It has been found that foodborne illnesses and associated discrepancies can impede the international trade of food commodities. Trade bans due to the fear of foodborne illnesses are growing. The consequences of foodborne diseases are multifaceted and include financial losses from trade restrictions, medical costs for prevention or control, resource depletion and a decline in food production. The overall effects are increased international trade tensions and livelihood vulnerability to poverty, notably for small-scale livestock producers. Potential food contaminants include microbes, pesticides, pharmaceutical residues, heavy metals and fraudulent such as improper food processing, mislabelling, poor packaging, adulteration and substitution. Hence, countries are encouraged to harmonize the rights and duties set by the World Trade Organization under sanitary and phytosanitarys to maximize their advantages in global markets. Based on this evidence, we recommend that each country develop and integrate regulations that would ensure the safety of both domestic and international food production systems. Furthermore, the global community should either revise the current functioning food regulatory and monitoring body or establish a more genuine collaborative network., (© 2024 The Author(s). Veterinary Medicine and Science published by John Wiley & Sons Ltd.)
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- 2024
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20. Identifying the impact of climate variables on biological and chemical contaminants of preharvest foods and their associated food safety risks: a scoping review protocol.
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Zai B, McReavy S, Hogan G, Ng V, Papadopoulos A, Young I, and Grant LE
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- Canada, Humans, Foodborne Diseases prevention & control, Foodborne Diseases epidemiology, Research Design, Climate Change, Food Safety, Food Contamination
- Abstract
Background: Foodborne and waterborne illnesses affect over four million Canadians annually and pose a preventable burden on the nation's healthcare system. Climate change can increase the risk of such illnesses by increasing the likelihood of exposure to contaminants. As climate change progresses, it is imperative to better understand its impact on the dissemination of foodborne and waterborne contaminants throughout the food system. Currently, there is limited, synthesised evidence for how future changes in Canada's climate may affect the risk of contamination of preharvest foods. The aim of this research is to collate and describe available information on effects of climate variables on biological and chemical contamination of preharvest foods in Canada. This information will contribute to improved understanding of climate change impacts and potential adaptation and mitigation strategies to increase climate resiliency in Canada's food system., Methods: A preliminary search of MEDLINE, Web of Science and Google was conducted to verify the absence of existing reviews and to inform the development of this review protocol. Information will be identified by searching four academic databases: MEDLINE via Ovid, AGRICultural OnLine Access (AGRICOLA), CAB International and Web of Science. This search will be supplemented by a targeted grey literature search. The search strategy includes index terms and keywords for Canada-relevant foodborne and waterborne pathogens and chemical contaminants, preharvest foods and climate change. Search results will be managed using Covidence during all phases of the review, conducted by two independent reviewers. Data will be extracted, synthesised and presented using graphical and tabular formats.This scoping review protocol describes the process for retrieving a comprehensive set of evidence for how climate change variables may increase risk of biological or chemical contamination of preharvest foods in Canada. This review will provide decision-makers with a detailed understanding of climate variable-preharvest food-contaminant combinations using the best available evidence., Ethics and Dissemination: Ethical considerations are not applicable to this protocol as scoping reviews conduct secondary data analysis that synthesises data from publicly available sources. The results from this review will be disseminated through a peer-reviewed publication and conference presentation., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2024. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)
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- 2024
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21. Responding to outbreaks of illness linked to unpasteurized milk: A needs assessment of state health and agriculture departments.
- Author
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Ablan M, Canning M, Koski L, Landsman L, Stapleton GS, Nichols M, and Robyn M
- Subjects
- Animals, Humans, United States epidemiology, Needs Assessment, State Government, Agriculture, Milk, Disease Outbreaks, Pasteurization, Foodborne Diseases epidemiology, Foodborne Diseases prevention & control
- Abstract
Aims: Consumption of unpasteurized milk can result in severe illness or death. In the United States, the number of people who regularly consume unpasteurized milk is relatively low, but outbreaks resulting from unpasteurized milk outnumber outbreaks linked to pasteurized milk. The sale of unpasteurized milk for human consumption through interstate commerce is prohibited at the federal level, but laws among states vary considerably with respect to the sale of unpasteurized milk. Each state has a different perspective on responding to and preventing outbreaks of illness linked to consuming unpasteurized milk., Methods and Results: We conducted a needs assessment of state health and agriculture departments to gather information on state-level strategies to prevent illnesses linked to consuming unpasteurized milk, characterize challenges states face, and identify areas where partners can support state efforts to prevent illnesses. We deployed a survey from 6 January 2021 to 1 March 2021, using a snowball sampling strategy and had 158 respondents. Of 115 respondents, 46 (40%) believed that state laws were ineffective in preventing illnesses, and 92 (80%) agreed that consumers continue to find ways to get unpasteurized milk despite laws restricting sale. Respondents from 19 states were aware of future legislative or regulatory efforts surrounding unpasteurized milk in their state, with 14 (74%) indicating these efforts would expand consumer access. The most common outbreak prevention strategies respondents mentioned included sharing knowledge and experiences with other public health and agriculture officials, providing information to inform legislative efforts, and communicating to the public about outbreaks. Most respondents (41/50, 91%) were interested in pursuing further efforts to prevent unpasteurized milk-associated illnesses in their state., Conclusions: The results from this needs assessment can be used to inform future strategies for preventing illness outbreaks associated with unpasteurized milk consumption., (Published 2024. This article is a U.S. Government work and is in the public domain in the USA.)
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- 2024
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22. Antimicrobial activities of natural flavonoids against foodborne pathogens and their application in food industry.
- Author
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Wu Y, Jiang L, Ran W, Zhong K, Zhao Y, and Gao H
- Subjects
- Food Industry, Food Microbiology, Anti-Infective Agents pharmacology, Anti-Infective Agents chemistry, Humans, Food Safety, Foodborne Diseases microbiology, Foodborne Diseases prevention & control, Flavonoids pharmacology, Flavonoids chemistry, Bacteria drug effects, Food Preservatives pharmacology, Food Preservatives chemistry
- Abstract
The application of natural alternatives as food preservatives has gained much attention due to the escalating negative perception of synthetic preservatives among consumers and the spread of drug-resistance foodborne pathogens. Natural flavonoids have the potential to be employed for food safety due to their antimicrobial properties against a wide range of foodborne pathogenic microorganisms. In this perspective, we reviewed the antimicrobial activities of natural flavonoids, the mechanism of action, as well as their application for food safety and quality. Various strategies for the incorporation of flavonoids into food products were highlighted, including direct addition to food formulations, encapsulation as micro or nanocarriers, and incorporation into edible or active films and coatings. Furthermore, we discussed the current challenges of industrial application of flavonoids, and proposed future trends to enhance their potential as natural preservatives. This review provides a theoretical foundation for the further development and application of flavonoids for food safety., Competing Interests: Declaration of competing interest The authors declare no conflict of interest., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
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- 2024
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23. Microplastics occurrence in sea cucumbers and impacts on sea cucumbers & human health: A systematic review.
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Bhuyan MS, Jenzri M, Pandit D, Adikari D, Alam MW, and Kunda M
- Subjects
- Animals, Humans, Ecosystem, Foodborne Diseases etiology, Foodborne Diseases prevention & control, Environmental Monitoring, Microplastics analysis, Microplastics poisoning, Microplastics toxicity, Sea Cucumbers chemistry, Sea Cucumbers drug effects, Sea Cucumbers physiology, Water Pollutants, Chemical analysis, Water Pollutants, Chemical poisoning, Water Pollutants, Chemical toxicity
- Abstract
Microplastics (MPs) are a developing concern in marine environments, with scientists concentrating more on their effects on various creatures. Sea cucumbers (SCs), as suspension and deposit feeders, are expected to be exposed to and consume MPs in their habitat. The purpose of this methodical review is to gather and integrate accessible research on the presence and effects of MPs on SCs. A systematic search of relevant databases yielded relevant papers exploring the occurrence of MPs in SC habitats as well as the possible effects of MP intake on SCs. Bibliometric analysis was also conducted to collect and analyze a large volume of data. Then the papers were sorted (a total of 249) related to the occurrence and effects of MPs in SCs. Finally, targeted data were collected from the articles for the study. The review emphasizes the ubiquity of MPs in SC ecosystems, citing studies that found high quantities in coastal areas and sediment. MPs have a variety of effects on SCs, with some studies indicating that they lower eating efficiency, affect behavior, and cause tissue damage. However, there is still no unanimity on the overall effects of MP exposure on SCs. This review gives a complete summary of the present state of information about the incidence and impact of MPs on SCs, highlighting the need for additional study in this area. Understanding the possible dangers of MPs on SCs is critical for the survival of these ecologically significant creatures., Competing Interests: Declaration of competing interest The authors claim that they have no known competing financial or personal interests that could have impacted the findings of this study., (Copyright © 2024 Elsevier B.V. All rights reserved.)
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- 2024
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24. An Experimental Test of the Effects of Public Mockery of a Social Media Health Campaign: Implications for Theory and Health Organizations' Social Media Strategies.
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Myrick JG, Chen J, Jang E, Norman MP, Liu Y, Medina L, Blessing JN, and Parhizkar H
- Subjects
- Humans, Female, United States, Male, Intention, Adult, Centers for Disease Control and Prevention, U.S., Foodborne Diseases prevention & control, Social Media, Health Promotion methods
- Abstract
This study explored how social media users' mocking of a public health campaign can affect other users' emotions, cognitions, and behavioral intentions. Inspired by public mocking of the CDC's "Say No to Raw Dough" campaign aiming to prevent food poisoning caused by eating raw flour-based products, this experiment ( N = 681) employed a 2 (Public responses to a PSA: Mocking or serious) x 3 (Organizational response to public responses: Self-mocking, serious, or none) + 1 (control condition) design. Statistical tests revealed that user-generated mocking can lower intentions to avoid the health risk by decreasing perceptions of injunctive norms (that is, seeing others mock a public health campaign resulted in weaker perceptions that others think you should avoid the risky behavior). Mockery of a public health campaign also engender anger at the CDC and at other users, with the target of the anger having differential effects on intentions to avoid eating raw dough. Implications for theory and the practice of social media-based health promotion are discussed.
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- 2024
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25. Bacteriophages: a potential game changer in food processing industry.
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Chaudhary V, Kajla P, Lather D, Chaudhary N, Dangi P, Singh P, and Pandiselvam R
- Subjects
- Food Safety, Food Handling, Foodborne Diseases microbiology, Foodborne Diseases prevention & control, Food-Processing Industry, Humans, Food Preservation methods, Food Industry, Bacteriophages physiology, Food Microbiology
- Abstract
In the food industry, despite the widespread use of interventions such as preservatives and thermal and non-thermal processing technologies to improve food safety, incidences of foodborne disease continue to happen worldwide, prompting the search for alternative strategies. Bacteriophages, commonly known as phages, have emerged as a promising alternative for controlling pathogenic bacteria in food. This review emphasizes the potential applications of phages in biological sciences, food processing, and preservation, with a particular focus on their role as biocontrol agents for improving food quality and preservation. By shedding light on recent developments and future possibilities, this review highlights the significance of phages in the food industry. Additionally, it addresses crucial aspects such as regulatory status and safety concerns surrounding the use of bacteriophages. The inclusion of up-to-date literature further underscores the relevance of phage-based strategies in reducing foodborne pathogenic bacteria's presence in both food and the production environment. As we look ahead, new phage products are likely to be targeted against emerging foodborne pathogens. This will further advance the efficacy of approaches that are based on phages in maintaining the safety and security of food.
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- 2024
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26. Inadequate food safety knowledge and hygiene practices among street food vendors in Dhaka, Bangladesh.
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Islam S, Tanjia N, Mitra AK, Hossain A, Jasika MT, Suhi SS, and Hossain SJ
- Subjects
- Humans, Bangladesh, Male, Female, Adult, Cross-Sectional Studies, Hygiene, Surveys and Questionnaires, Middle Aged, Young Adult, Food Safety, Health Knowledge, Attitudes, Practice, Food Handling standards, Foodborne Diseases prevention & control, Foodborne Diseases epidemiology
- Abstract
Food safety remains a critical issue with outbreaks of foodborne illness. The knowledge gap of food safety and improper hygienic practices of food handlers are the key factors for the transmission of foodborne diseases. This study was conducted to investigate the level of food safety knowledge and practices among street food vendors in Dhaka City, Bangladesh, and its implications on consumers' health. This cross-sectional study was conducted among 350 respondents in seven areas of Dhaka City. Trained data collectors gather data by interviewing street vendors using a pretested questionnaire. Most of the vendors (98%) were male, with 48% having secondary education and 85% having no food safety training. Although about 89% of the vendors were found to practice hand washing, only a small proportion of them practised using hand gloves while handling raw products (5.6%), cleaning tables (2.2%), preparing foods (1.3%) or handling garbage (0.9%). The education level of the vendors and their work experience were significantly correlated with their hand washing practice, wearing hand gloves and their knowledge about food-borne illnesses. The study demonstrated that formal education played a significant role in vendors' knowledge and practice of health safety measures for food handlers to prevent foodborne illness. Effective food safety training and monitoring are needed to increase vendors' knowledge and practices, and in reducing foodborne diseases., (© 2024. The Author(s).)
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- 2024
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27. Development of Stabilizing Solution for Long-Term Storage of Bacteriophages at Room Temperature and Application to Control Foodborne Pathogens.
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Kim EJ, Lim MC, Woo MA, Kim BS, and Lim JA
- Subjects
- Foodborne Diseases prevention & control, Foodborne Diseases microbiology, Food Safety, Bacteriophages physiology, Temperature, Food Microbiology, Vegetables microbiology, Vegetables virology, Escherichia coli O157 virology
- Abstract
Bacteriophages (phages) have gained considerable attention as effective antimicrobial agents that infect and kill pathogenic bacteria. Based on this feature, phages have been increasingly used to achieve food safety. They are stored in a medium or buffer to ensure stability; however, they cannot be directly applied to food under these conditions due to reasons such as regulatory considerations and concerns about marketability. This study developed a stabilizing solution that allowed the maintenance of phage activity for extended periods at room temperature while being directly applicable to food. The stability of phages stored in distilled water was relatively low. However, adding a stabilizer composed of sugars and salts improved the survival rates of phages significantly, resulting in stability for up to 48 weeks at room temperature. When Escherichia coli O157:H7-contaminated vegetables were washed with tap water containing phages, the phages reduced the pathogenic E. coli count by over 90% compared with washing with tap water alone. Additionally, when pathogenic E. coli -contaminated vegetables were placed in a phage-coated container and exposed to water, the coating of the container dissolved, releasing phages and lysing the pathogenic E. coli . This led to a significant 90% reduction in pathogenic E. coli contamination compared to that after water rinsing. These results suggest an effective and economical method for maintaining phage activity and establishing the potential for commercialization through application in the food industry.
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- 2024
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28. A One-Step RPA-CRISPR Assay Using crRNA Based on Suboptimal Protospacer Adjacent Motif for Vibrio vulnificus Detection.
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Zhang X, Wang Y, Tang Y, Yang L, Zhao C, Yang G, Wang P, and Gao S
- Subjects
- Food Microbiology, Seafood microbiology, Bacterial Proteins genetics, Bacterial Proteins metabolism, Clustered Regularly Interspaced Short Palindromic Repeats, Recombinases metabolism, Nucleic Acid Amplification Techniques methods, Foodborne Diseases microbiology, Foodborne Diseases prevention & control, CRISPR-Associated Proteins genetics, CRISPR-Associated Proteins metabolism, Sensitivity and Specificity, Vibrio vulnificus isolation & purification, Vibrio vulnificus genetics, CRISPR-Cas Systems
- Abstract
Vibrio vulnificus is a hazardous foodborne pathogen responsible for approximately 95% of seafood-related deaths. This highlights the urgent requirement for specialized detection tools to be developed and used by food enterprises and food safety authorities. The DETECTR (DNA endonuclease targeted CRISPR trans reporter) system that combines CRISPR/Cas and recombinase polymerase amplification (RPA) has been utilized to develop a molecular detection assay for V. vulnificus . However, because the incompatibility between RPA and Cas12a cleavage has not been addressed, it is a two-step assay that lacks convenience and presents contamination risk. Here, we developed a one-step RPA-CRISPR assay for V. vulnificus using a special crRNA targeting a sequence with a suboptimal protospacer adjacent motif (PAM). The entire assay, conducted at 37°C, takes only 40-60 min, yields results visualized under blue light, and exhibits exceptional specificity and sensitivity (detecting 4 pathogen genome copies per reaction). This study offers a valuable tool for detecting V. vulnificus , aiding in foodborne infection prevention, and exemplifies one-step RPA-CRISPR assays managing Cas-cleavage activity through PAM adjustments.
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- 2024
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29. Interference of gastrointestinal barriers with antibiotic susceptibility of foodborne pathogens: an in vitro case study of ciprofloxacin and tetracycline against Salmonella enterica and Listeria monocytogenes.
- Author
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Gaspari S, Akkermans S, Akritidou T, Whelan R, Devine F, and Van Impe JFM
- Subjects
- Humans, Gastrointestinal Tract microbiology, Gastrointestinal Microbiome drug effects, Food Microbiology, Hydrogen-Ion Concentration, Foodborne Diseases microbiology, Foodborne Diseases prevention & control, Ciprofloxacin pharmacology, Listeria monocytogenes drug effects, Salmonella enterica drug effects, Tetracycline pharmacology, Anti-Bacterial Agents pharmacology, Microbial Sensitivity Tests
- Abstract
Minimum inhibitory concentrations (MIC) assays are often questioned for their representativeness. Especially when foodborne pathogens are tested, it is of crucial importance to also consider parameters of the human digestive system. Hence, the current study aimed to assess the inhibitory capacity of two antibiotics, ciprofloxacin and tetracycline, against Salmonella enterica and Listeria monocytogenes, under representative environmental conditions. More specifically, aspects of the harsh environment of the human gastrointestinal tract (GIT) were gradually added to the experimental conditions starting from simple aerobic lab conditions into an in vitro simulation of the GIT. In this way, the effects of parameters including the anoxic environment, physicochemical conditions of the GIT (low gastric pH, digestive enzymes, bile acids) and the gut microbiota were evaluated. The latter was simulated by including a representative consortium of selected gut bacteria species. In this study, the MIC of the two antibiotics against the relevant foodborne pathogens were established, under the previously mentioned environmental conditions. The results of S. enterica highlighted the importance of the anaerobic environment when conducting such studies, since the pathogen thrived under such conditions. Inclusion of physicochemical barriers led to exactly opposite results for S. enterica and L. monocytogenes since the former became more susceptible to ciprofloxacin while the latter showed lower susceptibility towards tetracycline. Finally, the inclusion of gut bacteria had a bactericidal effect against L. monocytogenes even in the absence of antibiotics, while gut bacteria protected S. enterica from the effect of ciprofloxacin., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Ltd.)
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- 2024
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30. Comprehensive analysis of predominant pathogenic bacteria and viruses in seafood products.
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Roy PK, Roy A, Jeon EB, DeWitt CAM, Park JW, and Park SY
- Subjects
- Humans, Food Safety, Food Contamination analysis, Foodborne Diseases microbiology, Foodborne Diseases epidemiology, Foodborne Diseases prevention & control, Foodborne Diseases virology, Animals, Food Handling methods, Seafood microbiology, Food Microbiology, Bacteria isolation & purification, Viruses isolation & purification
- Abstract
Given the growing global demand for seafood, it is imperative to conduct a comprehensive study on the prevalence and persistence patterns of pathogenic bacteria and viruses associated with specific seafood varieties. This assessment thoroughly examines the safety of seafood products, considering the diverse processing methods employed in the industry. The importance of understanding the behavior of foodborne pathogens, such as Salmonella typhimurium, Vibrio parahaemolyticus, Clostridium botulinum, Listeria monocytogenes, human norovirus, and hepatitis A virus, is emphasized by recent cases of gastroenteritis outbreaks linked to contaminated seafood. This analysis examines outbreaks linked to seafood in the United States and globally, with a particular emphasis on the health concerns posed by pathogenic bacteria and viruses to consumers. Ensuring the safety of seafood is crucial since it directly relates to consumer preferences on sustainability, food safety, provenance, and availability. The review focuses on assessing the frequency, growth, and durability of infections that arise during the processing of seafood. It utilizes next-generation sequencing to identify the bacteria responsible for these illnesses. Additionally, it analyzes methods for preventing and intervening of infections while also considering the forthcoming challenges in ensuring the microbiological safety of seafood products. This evaluation emphasizes the significance of the seafood processing industry in promptly responding to evolving consumer preferences by offering current information on seafood hazards and future consumption patterns. To ensure the continuous safety and sustainable future of seafood products, it is crucial to identify and address possible threats., (© 2024 The Author(s). Comprehensive Reviews in Food Science and Food Safety published by Wiley Periodicals LLC on behalf of Institute of Food Technologists.)
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- 2024
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31. Assessment of the Microbiological Quality and Effect of Public Health of Ready-to-Eat Salad Samples in Isparta.
- Author
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Yamaner Ç and Sürücü N
- Subjects
- Humans, Turkey, Salads microbiology, Food Contamination analysis, Microbial Sensitivity Tests, Fast Foods microbiology, Anti-Bacterial Agents pharmacology, Colony Count, Microbial, Citrobacter freundii isolation & purification, Foodborne Diseases microbiology, Foodborne Diseases prevention & control, Drug Resistance, Bacterial, Salmonella isolation & purification, Food Microbiology, Citrobacter isolation & purification, Public Health
- Abstract
Salmonella spp. and Citrobacter spp. are among the microorganisms causing important foodborne outbreaks. In this study, it was tried to determine the presence and rate of Salmonella spp. and Citrobacter spp. in salad samples collected from certain regions of province of Isparta in Türkiye. A total of 50 salad samples were analyzed. Classical culture technique was used for microbiological analysis of salad samples. Suspected isolates obtained were identified using the VITEK-2 system. Although no negative visual changes were observed in the salad samples used in the study, it was determined that the number of Gram-negative microorganisms was very high and six salad samples were not suitable for public health. In 50 salad samples, 2% Salmonella and 4% Citrobacter freundii were detected. In addition, it was determined that the Salmonella strain isolated from the salad sample was resistant to three different antibiotics and Citrobacter was resistant to two different antibiotics. Salmonella spp. and Citrobacter spp. are considered very dangerous to public health because they are associated with foodborne outbreaks and can develop antibiotic resistance very quickly. Salad producers should try to reduce the possibility of microbial contamination by using different technologies.
- Published
- 2024
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32. The Influence of Simulated Organic Matter on the Inactivation of Viruses: A Review.
- Author
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Allingham C, Taniguchi M, Kinchla AJ, and Moore MD
- Subjects
- Humans, Food Microbiology, Disinfection methods, Foodborne Diseases prevention & control, Foodborne Diseases virology, Organic Chemicals pharmacology, Organic Chemicals chemistry, Virus Inactivation drug effects, Disinfectants pharmacology, Viruses drug effects
- Abstract
Viruses impose a significant public health burden globally, and one of the key elements in controlling their transmission is the ability to inactivate them using disinfectants. However, numerous challenges to inactivating foodborne viruses exist due to inherent viral characteristics (such as recalcitrance to commonly used inactivation agents) and external factors (such as improper cleaning before application of inactivation agent, improper contact time, etc.). Given the potential for improper application of disinfectants (such as shorter than recommended contact time, improper disinfectant concentration, etc.), understanding the performance of a disinfectant in the presence of an organic load is important. To accomplish this, the introduction of simulated organic loads is often used when studying the efficacy of a disinfectant against different viruses. However, the different types of simulated organic loads used in foodborne virus inactivation studies or their relative effects on inactivation have not been reviewed. The purpose of this review is to survey different simulated organic load formulations used in studying foodborne virus inactivation, as well as present and compare the influence of these different formulations on viral inactivation. The findings included in this review suggest that many simulated organic load formulations can reduce disinfectants' efficacy against viruses. Based on the findings in this review, blood, particularly serum or feces, are among the most commonly used and efficacious forms of simulated organic load in many tests.
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- 2024
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33. CRISPR/Cas-based colorimetric biosensors: a promising tool for the diagnosis of bacterial foodborne pathogens in food products.
- Author
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Saleh EAM, Ali E, Muxamadovna GM, Kassem AF, Kaur I, Kumar A, Jabbar HS, Alwaily ER, Elawady A, and Omran AA
- Subjects
- Humans, Bacteria isolation & purification, Bacteria genetics, Biosensing Techniques methods, Colorimetry methods, CRISPR-Cas Systems, Food Microbiology methods, Foodborne Diseases diagnosis, Foodborne Diseases microbiology, Foodborne Diseases prevention & control
- Abstract
Some physical phenomena and various chemical substances newly introduced in nanotechnology have allowed scientists to develop valuable devices in the field of food sciences. Regarding such progress, the identification of foodborne pathogenic microorganisms is an imperative subject nowadays. These bacterial species have been found to cause severe health impacts after food ingestion and can result in high mortality in acute cases. The rapid detection of foodborne bacterial species at low concentrations is in high demand in recent diagnostics. CRISPR/Cas-mediated biosensors possess the potential to overcome several challenges in classical assays such as complex pretreatments, long turnaround time, and insensitivity. Among them, colorimetric nanoprobes based on the CRISPR strategy afford promising devices for POCT (point-of-care testing) since they can be visualized with the naked eye and do not require diagnostic apparatus. In this study, we briefly classify and discuss the working principles of the different CRISPR/Cas protein agents that have been employed in biosensors so far. We assess the current status of the CRISPR system, specifically focusing on colorimetric biosensing platforms. We discuss the utilization of each Cas effector in the detection of foodborne pathogens and examine the restrictions of the existing technology. The challenges and future opportunities are also indicated and addressed.
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- 2024
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34. Machine learning approach as an early warning system to prevent foodborne Salmonella outbreaks in northwestern Italy.
- Author
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Garcia-Vozmediano A, Maurella C, Ceballos LA, Crescio E, Meo R, Martelli W, Pitti M, Lombardi D, Meloni D, Pasqualini C, and Ru G
- Subjects
- Italy epidemiology, Humans, Animals, Salmonella physiology, Food Microbiology, Foodborne Diseases prevention & control, Foodborne Diseases epidemiology, Foodborne Diseases microbiology, Prevalence, Salmonella Infections epidemiology, Salmonella Infections prevention & control, Machine Learning, Disease Outbreaks veterinary, Disease Outbreaks prevention & control, Salmonella Food Poisoning prevention & control, Salmonella Food Poisoning epidemiology
- Abstract
Salmonellosis, one of the most common foodborne infections in Europe, is monitored by food safety surveillance programmes, resulting in the generation of extensive databases. By leveraging tree-based machine learning (ML) algorithms, we exploited data from food safety audits to predict spatiotemporal patterns of salmonellosis in northwestern Italy. Data on human cases confirmed in 2015-2018 (n = 1969) and food surveillance data collected in 2014-2018 were used to develop ML algorithms. We integrated the monthly municipal human incidence with 27 potential predictors, including the observed prevalence of Salmonella in food. We applied the tree regression, random forest and gradient boosting algorithms considering different scenarios and evaluated their predictivity in terms of the mean absolute percentage error (MAPE) and R
2 . Using a similar dataset from the year 2019, spatiotemporal predictions and their relative sensitivities and specificities were obtained. Random forest and gradient boosting (R2 = 0.55, MAPE = 7.5%) outperformed the tree regression algorithm (R2 = 0.42, MAPE = 8.8%). Salmonella prevalence in food; spatial features; and monitoring efforts in ready-to-eat milk, fruits and vegetables, and pig meat products contributed the most to the models' predictivity, reducing the variance by 90.5%. Conversely, the number of positive samples obtained for specific food matrices minimally influenced the predictions (2.9%). Spatiotemporal predictions for 2019 showed sensitivity and specificity levels of 46.5% (due to the lack of some infection hotspots) and 78.5%, respectively. This study demonstrates the added value of integrating data from human and veterinary health services to develop predictive models of human salmonellosis occurrence, providing early warnings useful for mitigating foodborne disease impacts on public health., (© 2024. The Author(s).)- Published
- 2024
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35. Antibacterial effectiveness of trans-cinnamaldehyde against foodborne Enterobacteriaceae and its adjuvant effect with gentamicin.
- Author
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da Silva BF, Pereira AMG, Coelho PAT, de Almeida MVA, Dos Santos HS, Carneiro VA, and Costa RA
- Subjects
- Animals, Food Microbiology, Foodborne Diseases microbiology, Foodborne Diseases prevention & control, Anti-Bacterial Agents pharmacology, Gentamicins pharmacology, Microbial Sensitivity Tests, Enterobacteriaceae drug effects, Enterobacteriaceae growth & development, Acrolein analogs & derivatives, Acrolein pharmacology, Drug Synergism
- Abstract
The Enterobacteriaceae family is recognized as a primary group of Gram-negative pathogens responsible for foodborne illnesses and is frequently associated with antibiotic resistance. The present study explores the natural-based compound trans-cinnamaldehyde (TC) against drug-resistant Enterobacteriaceae and its synergism with gentamicin (GEN) to address this issue. The research employs three strains of Escherichia coli, Klebsiella pneumoniae, and Enterobacter cloacae, previously isolated from shrimp. The antibacterial activity was evaluated by the disk diffusion method, microdilution test, kinetics of growth, and time-kill curve. In addition, the synergistic effect between TC/GEN was investigated by checkerboard assay. All strains showed sensitivity to TC with an inhibition zone diameter > 35 mm. The TC showed inhibitory and bactericidal action in the most tested bacteria around 625 μg/mL. Sub-inhibitory amounts (1/2 and 1/4 MIC) of TC interfered with the growth kinetics by lag phase extension and decreased the log phase. Time-kill curves show a reduction of viable cells after the first hour of TC treatment at bactericidal concentrations. The synergistic effect between TC/GEN was observed for E. coli and E. cloacae strains with FICi ranging from 0.15 to 0.50. These findings, therefore, suggest TC as a promising alternative in the fight against drug-resistant Enterobacteriaceae that can cause foodborne illnesses., (© 2024. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
- Published
- 2024
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36. Characterization of Antimicrobial Activities of Bifidobacterium lactis BB-12 and Their Inhibitory Effect Against Some Foodborne Pathogens.
- Author
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Roozbahani F, Ahanjan M, Moshiri M, Abediankenari S, Goli HR, Kakavan M, and Gholami M
- Subjects
- Antibiosis, Foodborne Diseases prevention & control, Foodborne Diseases microbiology, Anti-Bacterial Agents pharmacology, Listeria monocytogenes drug effects, Probiotics pharmacology, Salmonella typhimurium drug effects, Salmonella typhimurium growth & development, Microbial Sensitivity Tests, Bifidobacterium animalis, Food Microbiology
- Abstract
Bifidobacterium animalis subsp . lactis BB-12, a probiotic, has shown potential to promote health benefits and control pathogens. This study aimed to investigate the effectiveness of BB-12 and its cell-free supernatant (CFS) in inhibiting the growth of Listeria monocytogenes and Salmonella enterica serovar Typhimurium. To assess the antimicrobial activity of BB-12, agar well diffusion, disk diffusion, and minimum inhibitory concentration (MIC) tests were conducted. The bicinchoninic acid (BCA) assay was performed to measure the protein concentration in CFS. The study's results indicated that the BB-12 strain inhibited the pathogens' growth. The disk diffusion test using BB-12 showed inhibitory results ranging from 11 to 14 mm for both bacteria. The agar well diffusion test reported the zone of inhibition ranging from 11.6 to 16 mm for both bacteria. The MIC test was conducted as a confirmatory test, which demonstrated the highest inhibitory zone using 2 McFarland (6 × 10
8 CFU/mL) concentrations of probiotics on L. monocytogenes (44.98%) and S. Typhimurium (66.41%). The disk diffusion test revealed that the probiotic CFS had a significant inhibitory impact on S. Typhimurium with a 16.6 mm zone of inhibition. The BCA test findings indicated that the 24- and 48-h CFSs exhibited inhibitory properties against infections. Notably, the 24-h CFS, including a protein level of 78.47 μg/mL, demonstrated a more pronounced inhibitory impact on both pathogens. The findings highlight that utilizing the BB-12 strain and its CFS can serve as a viable approach to battle infections, enhancing food safety and public health.- Published
- 2024
- Full Text
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37. Degradation of Toxins Derived from Foodborne Pathogens by Atmospheric-Pressure Dielectric-Barrier Discharge.
- Author
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Sakudo A and Yagyu Y
- Subjects
- Enterotoxins, Depsipeptides chemistry, Food Microbiology methods, Chromatography, Liquid methods, Foodborne Diseases prevention & control, Foodborne Diseases microbiology, Enzyme-Linked Immunosorbent Assay, Food Contamination analysis, Plasma Gases chemistry, Aflatoxin B1, Atmospheric Pressure, Tandem Mass Spectrometry
- Abstract
Foodborne diseases can be attributed not only to contamination with bacterial or fungal pathogens but also their associated toxins. Thus, to maintain food safety, innovative decontamination techniques for toxins are required. We previously demonstrated that an atmospheric-pressure dielectric-barrier discharge (APDBD) plasma generated by a roller conveyer plasma device is effective at inactivating bacteria and fungi in foods. Here, we have further examined whether the roller conveyer plasma device can be used to degrade toxins produced by foodborne bacterial pathogens, including aflatoxin, Shiga toxins (Stx1 and Stx2), enterotoxin B and cereulide. Each toxin was spotted onto an aluminum plate, allowed to dry, and then treated with APDBD plasma applied by the roller conveyer plasma device for different time periods. Assessments were conducted using a competitive enzyme-linked immunosorbent assay (ELISA) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The results demonstrate a significant time-dependent decrease in the levels of these toxins. ELISA showed that aflatoxin B
1 concentrations were reduced from 308.6 µg/mL to 74.4 µg/mL within 1 min. For Shiga toxins, Stx1 decreased from 913.8 µg/mL to 65.1 µg/mL, and Stx2 from 2309.0 µg/mL to 187.6 µg/mL within the same time frame (1 min). Enterotoxin B levels dropped from 62.67 µg/mL to 1.74 µg/mL at 15 min, and 1.43 µg/mL at 30 min, but did not display a significant decrease within 5 min. LC-MS/MS analysis verified that cereulide was reduced to below the detection limit following 30 min of APDBD plasma treatment. Taken together, these findings highlight that a range of foodborne toxins can be degraded by a relatively short exposure to plasma generated by an APDBD using a roller conveyer device. This technology offers promising advancements in food safety, providing a novel method to alleviate toxin contamination in the food processing industry.- Published
- 2024
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38. Multimodal Biosensing of Foodborne Pathogens.
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Ullah N, Bruce-Tagoe TA, Asamoah GA, and Danquah MK
- Subjects
- Humans, Food Safety methods, Biosensing Techniques methods, Foodborne Diseases microbiology, Foodborne Diseases diagnosis, Foodborne Diseases prevention & control, Food Microbiology methods
- Abstract
Microbial foodborne pathogens present significant challenges to public health and the food industry, requiring rapid and accurate detection methods to prevent infections and ensure food safety. Conventional single biosensing techniques often exhibit limitations in terms of sensitivity, specificity, and rapidity. In response, there has been a growing interest in multimodal biosensing approaches that combine multiple sensing techniques to enhance the efficacy, accuracy, and precision in detecting these pathogens. This review investigates the current state of multimodal biosensing technologies and their potential applications within the food industry. Various multimodal biosensing platforms, such as opto-electrochemical, optical nanomaterial, multiple nanomaterial-based systems, hybrid biosensing microfluidics, and microfabrication techniques are discussed. The review provides an in-depth analysis of the advantages, challenges, and future prospects of multimodal biosensing for foodborne pathogens, emphasizing its transformative potential for food safety and public health. This comprehensive analysis aims to contribute to the development of innovative strategies for combating foodborne infections and ensuring the reliability of the global food supply chain.
- Published
- 2024
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39. Food-Borne Biotoxin Neutralization in Vivo by Nanobodies: Current Status and Prospects.
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Liu ML, Liang XM, Jin MY, Huang HW, Luo L, Wang H, Shen X, and Xu ZL
- Subjects
- Animals, Humans, Food Contamination analysis, Food Contamination prevention & control, Antibodies, Neutralizing immunology, Toxins, Biological immunology, Foodborne Diseases prevention & control, Foodborne Diseases immunology, Camelus immunology, Single-Domain Antibodies immunology, Single-Domain Antibodies chemistry, Single-Domain Antibodies genetics
- Abstract
Food-borne biotoxins from microbes, plants, or animals contaminate unclean, spoiled, and rotten foods, posing significant health risks. Neutralizing such toxins is vital for human health, especially after food poisoning. Nanobodies (Nbs), a type of single-domain antibodies derived from the genetic cloning of a variable domain of heavy chain antibodies (VHHs) in camels, offer unique advantages in toxin neutralization. Their small size, high stability, and precise binding enable effective neutralization. The use of Nbs in neutralizing food-borne biotoxins offers numerous benefits, and their genetic malleability allows tailored optimization for diverse toxins. As nanotechnology continues to evolve and improve, Nbs are poised to become increasingly efficient and safer tools for toxin neutralization, playing a pivotal role in safeguarding human health and environmental safety. This review not only highlights the efficacy of these agents in neutralizing toxins but also proposes innovative solutions to address their current challenges. It lays a solid foundation for their further development in this crucial field and propels their commercial application, thereby contributing significantly to advancements in this domain.
- Published
- 2024
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40. A Reliable Multifaceted Solution against Foodborne Viral Infections: The Case of RiLK1 Decapeptide.
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Galatola E, Agrillo B, Gogliettino M, Palmieri G, Maccaroni S, Vicenza T, Proroga YTR, Mancusi A, Di Pasquale S, Suffredini E, and Cozzi L
- Subjects
- Animals, Humans, Mice, Antimicrobial Peptides pharmacology, Antimicrobial Peptides chemistry, Hepatitis A virus drug effects, Microbial Sensitivity Tests, Norovirus drug effects, Virus Diseases prevention & control, Antiviral Agents pharmacology, Antiviral Agents chemistry, Foodborne Diseases prevention & control
- Abstract
Food-borne transmission is a recognized route for many viruses associated with gastrointestinal, hepatic, or neurological diseases. Therefore, it is essential to identify new bioactive compounds with broad-spectrum antiviral activity to exploit innovative solutions against these hazards. Recently, antimicrobial peptides (AMPs) have been recognized as promising antiviral agents. Indeed, while the antibacterial and antifungal effects of these molecules have been widely reported, their use as potential antiviral agents has not yet been fully investigated. Herein, the antiviral activity of previously identified or newly designed AMPs was evaluated against the non-enveloped RNA viruses, hepatitis A virus (HAV) and murine norovirus (MNV), a surrogate for human norovirus. Moreover, specific assays were performed to recognize at which stage of the viral infection cycle the peptides could function. The results showed that almost all peptides displayed virucidal effects, with about 90% of infectivity reduction in HAV or MNV. However, the decapeptide RiLK1 demonstrated, together with its antibacterial and antifungal properties, a notable reduction in viral infection for both HAV and MNV, possibly through direct interaction with viral particles causing their damage or hindering the recognition of cellular receptors. Hence, RiLK1 could represent a versatile antimicrobial agent effective against various foodborne pathogens including viruses, bacteria, and fungi.
- Published
- 2024
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41. Designing a Multiepitope Vaccine against the Foodborne Pathogenic Bacteria Listeria monocytogenes Using Subtractive Immunoinformatics Approaches.
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Aziz T, Naveed M, Shabbir MA, Jabeen K, Khan AA, Hasnain A, Yang Z, Zinedine A, Rocha JM, and Albekairi TH
- Subjects
- Epitopes, B-Lymphocyte immunology, Epitopes, B-Lymphocyte chemistry, Humans, Epitopes immunology, Molecular Dynamics Simulation, Animals, Foodborne Diseases prevention & control, Foodborne Diseases microbiology, Foodborne Diseases immunology, Immunoinformatics, Listeria monocytogenes immunology, Molecular Docking Simulation, Bacterial Vaccines immunology, Vaccines, Subunit immunology, Listeriosis prevention & control, Listeriosis immunology, Listeriosis microbiology, Computational Biology methods
- Abstract
Background: Listeria monocytogenes , a Gram-positive bacterium, is a prominent foodborne pathogen that causes listeriosis and poses substantial health hazards worldwide. The continuing risk of listeriosis outbreaks underlies the importance of designing an effective prevention strategy and developing a robust immune response by reverse vaccinology approaches. This study aimed to provide a critical approach for developing a potent multiepitope vaccine against this foodborne disease., Methods: A chimeric peptide construct containing 5 B-cell epitopes, 16 major histocompatibility complex I (MHC-I) epitopes, and 18 MHC-II epitopes were used to create a subunit vaccination against L. monocytogenes . The vaccine safety was evaluated by several online methods, and molecular docking was performed using ClusPro to determine the binding affinity. Immune simulation was performed using the C-ImmSimm server to demonstrate the immune response., Results: The results validated the antigenicity, non-allergenicity, and nontoxicity of the chimeric peptide construct, confirming its suitability as a subunit vaccine. Molecular docking showed a good score of 1276.5 and molecular dynamics simulations confirmed the construct's efficacy, demonstrating its promise as a good candidate for listeriosis prophylaxis. The population coverage was as high as 91.04% with a good immune response, indicating good antigen presentation with dendritic cells and production of memory cells., Conclusions: The findings of this study highlight the potential of the designed chimeric peptide construct as an effective subunit vaccine against Listeria , paving the way for future advances in preventive methods and vaccine design., Competing Interests: The authors declare no conflict of interest., (© 2024 The Author(s). Published by IMR Press.)
- Published
- 2024
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42. Nanomaterials with Enzyme-like Properties for Combatting Foodborne Pathogen Infections: Classifications, Mechanisms, and Applications in Food Preservation.
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Cui F, Li L, Wang D, Li J, and Li T
- Subjects
- Humans, Foodborne Diseases prevention & control, Foodborne Diseases microbiology, Food Microbiology, Biofilms drug effects, Food Preservation methods, Nanostructures chemistry, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents chemistry, Bacteria drug effects, Bacteria genetics, Bacteria classification
- Abstract
During the transportation and storage of food, foodborne spoilage caused by bacterial and biofilm infection is prone to occur, leading to issues such as short shelf life, economic loss, and sensory quality instability. Therefore, the development of novel and efficient antibacterial agents capable of efficiently inhibiting bacteria throughout various stages of food processing, transportation, and storage is strongly recommended by researchers. The emergence of nanozymes is considered to be an effective candidate for inhibiting foodborne bacteria agents in the food industry. As potent antibacterial agents, nanozymes have the advantages of low cost, high stability, strong broad-spectrum antibacterial ability, and biocompatibility. Herein, we aim to summarize the classification status of various nanozymes. Furthermore, the general catalytic bacteriostatic mechanism of nanozymes against intracellular bacteria, planktonic bacteria, and biofilm activities are highlighted, mainly concerning the destruction of cell walls and/or membranes, reactive oxygen species regulation, HOBr/Cl generation, damage of intracellular components, and so forth. In particular, the review focuses on the pivotal role of nanozymes as antibacterial agents and delivery vehicles in the fields of food preservation applications. We look forward to the future prospects, especially in the field of food preservation, to promote broader applications based on antimicrobial nanozymes.
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- 2024
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43. Systematic review on microbiome-related nutritional interventions interfering with the colonization of foodborne pathogens in broiler gut to prevent contamination of poultry meat.
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Mekonnen YT, Savini F, Indio V, Seguino A, Giacometti F, Serraino A, Candela M, and De Cesare A
- Subjects
- Animals, Meat analysis, Probiotics administration & dosage, Probiotics pharmacology, Animal Feed analysis, Food Microbiology, Foodborne Diseases veterinary, Foodborne Diseases prevention & control, Foodborne Diseases microbiology, Diet veterinary, Chickens, Gastrointestinal Microbiome, Poultry Diseases prevention & control, Poultry Diseases microbiology
- Abstract
This systematic review aimed to compile the available body of knowledge about microbiome-related nutritional interventions contributing to improve the chicken health and having an impact on the reduction of colonization by foodborne pathogens in the gut. Original research articles published between 2012 and 2022 were systematically searched in Scopus and PubMed. A total of 1,948 articles were retrieved and 140 fulfilled the inclusion criteria. Overall, 73 papers described 99 interventions against colonization by Escherichia coli and related organisms; 10 papers described 15 interventions against Campylobacter spp.; 36 papers described 54 interventions against Salmonella; 40 papers described 54 interventions against Clostridium perfringens. A total of 197 microbiome-related interventions were identified as effective against one or more of the listed pathogens and included probiotics (n = 80), prebiotics (n = 23), phytobiotics (n = 25), synbiotics (n = 12), organic acids (n = 12), enzymes (n = 4), essential oils (n = 14) and combination of these (n = 27). The identified interventions were mostly administered in the feed (173/197) or through oral gavage (11/197), in the drinking water (7/197), in ovo (2/197), intra amniotic (2/197), in fresh or reused litter (1/197) or both in the feed and water (1/197). The interventions enhanced the beneficial microbial communities in the broiler gut as Lactic acid bacteria, mostly Lactobacillus spp., or modulated multiple microbial populations. The mechanisms promoting the fighting against colonization by foodborne pathogens included competitive exclusion, production of short chain fatty acids, decrease of gut pH, restoration of the microbiome after dysbiosis events, promotion of a more stable microbial ecology, expression of genes improving the integrity of intestinal mucosa, enhancing of mucin production and improvement of host immune response. All the studies extracted from the literature described in vivo trials but performed on a limited number of animals under experimental settings. Moreover, they detailed the effect of the intervention on the chicken gut without details on further impact on poultry meat safety., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2024
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44. The Knowledge and Practices Toward Food Safety Measures at Home in the Lebanese Community.
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Annan M, Sakr S, Alaouie Z, Salla M, Sheet I, and Al Khatib A
- Subjects
- Female, Child, Humans, Cross-Sectional Studies, Food Handling methods, Cooking methods, Health Knowledge, Attitudes, Practice, Food Safety methods, Foodborne Diseases prevention & control, Foodborne Diseases epidemiology, Foodborne Diseases etiology
- Abstract
Hygienic measures practiced at home are highly related to the occurrence of food-borne diseases during food production, storage, and handling. Contaminated food remains a major cause of several diarrheal diseases, hospitalizations, and spikes in medical expenses. In our current study, we aimed to assess the knowledge of food safety and the food safety and hygiene practices at home among the Lebanese population. A cross-sectional study was conducted through an online questionnaire including two sections. The first section included socio-demographic characteristics of participants, whereas the second section included questions related to practices and knowledge about food safety, divided into five parts; personal hygiene practices, dry and cold storage, sanitizing and cleaning and food intoxication. A total of 1101 Lebanese above 18 years participated and provided their responses to the questionnaire. Overall, the majority of participants had fair knowledge about food safety where 96.8% of the participants answered correctly about preventing microbial growth on food. 77.9% of those participants acquired their knowledge about food safety from articles, workshops, or the internet. Moreover, females, people with children and those who cook for themselves scored significantly higher than others (68.8, 70.6, and 70%, respectively). In comparison to younger participants (67.8%), older participants (50+ and 30-49) scored higher at 69.7% and 68.9%, respectively. Higher scores were obtained for questions related to storing dried foods/meat and poultry products with percentages 91.4 and 87.8%, respectively. However, lower scores were noticed on questions related to washing raw chicken before handling and storing eggs (9.7 and 12.3%, respectively). Altogether, our results revealed the need for directed food safety awareness campaigns at the national level to educate the Lebanese community about domestic food handling practices. We believe these campaigns can significantly reduce related diseases and hospitalizations.
- Published
- 2024
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45. Optical sensing for real-time detection of food-borne pathogens in fresh produce using machine learning.
- Author
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Sharma S and Tharani L
- Subjects
- Foodborne Diseases microbiology, Foodborne Diseases prevention & control, Food Safety methods, Humans, Vegetables microbiology, Food Contamination analysis, Salmonella isolation & purification, Machine Learning, Food Microbiology methods, Escherichia coli isolation & purification
- Abstract
Contaminated fresh produce remains a prominent catalyst for food-borne illnesses, prompting the need for swift and precise pathogen detection to mitigate health risks. This paper introduces an innovative strategy for identifying food-borne pathogens in fresh produce samples from local markets and grocery stores, utilizing optical sensing and machine learning. The core of our approach is a photonics-based sensor system, which instantaneously generates optical signals to detect pathogen presence. Machine learning algorithms process the copious sensor data to predict contamination probabilities in real time. Our study reveals compelling results, affirming the efficacy of our method in identifying prevalent food-borne pathogens, including Escherichia coli ( E. coli ) and Salmonella enteric , across diverse fresh produce samples. The outcomes underline our approach's precision, achieving detection accuracies of up to 95%, surpassing traditional, time-consuming, and less accurate methods. Our method's key advantages encompass real-time capabilities, heightened accuracy, and cost-effectiveness, facilitating its adoption by both food industry stakeholders and regulatory bodies for quality assurance and safety oversight. Implementation holds the potential to elevate food safety and reduce wastage. Our research signifies a substantial stride toward the development of a dependable, real-time food safety monitoring system for fresh produce. Future research endeavors will be dedicated to optimizing system performance, crafting portable field sensors, and broadening pathogen detection capabilities. This novel approach promises substantial enhancements in food safety and public health., Competing Interests: Declaration of conflicting interestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
- Published
- 2024
- Full Text
- View/download PDF
46. Phage cocktails - an emerging approach for the control of bacterial infection with major emphasis on foodborne pathogens.
- Author
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Teklemariam AD, Al Hindi R, Qadri I, Alharbi MG, Hashem AM, Alrefaei AA, Basamad NA, Haque S, Alamri T, and Harakeh S
- Subjects
- Humans, Bacteria virology, Phage Therapy methods, Bacteriophages, Foodborne Diseases therapy, Foodborne Diseases microbiology, Foodborne Diseases prevention & control, Bacterial Infections therapy
- Abstract
Phage therapy has recently attracted a great deal of attention to counteract the rapid emergence of antibiotic-resistant bacteria. In comparison to monophage therapy, phage cocktails are typically used to treat individual and/or multi-bacterial infections since the bacterial agents are unlikely to become resistant as a result of exposure to multiple phages simultaneously. The bacteriolytic effect of phage cocktails may produce efficient killing effect in comparison to individual phage. However, multiple use of phages (complex cocktails) may lead to undesirable side effects such as dysbiosis, horizontal gene transfer, phage resistance, cross resistance, and/or higher cost of production. Cocktail formulation, therefore, representa compromise between limiting the complexity of the cocktail and achieving substantial bacterial load reduction towards the targeted host organisms. Despite some constraints, the applications of monophage therapy have been well documented in the literature. However, phage cocktails-based approaches and their role for the control of pathogens have not been well investigated. In this review, we discuss the principle of phage cocktail formulations, their optimization strategies, major phage cocktail preparations, and their efficacy in inactivating various food borne bacterial pathogens.
- Published
- 2024
- Full Text
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47. Impact of Foodborne Disease in Taiwan during the COVID-19 Pandemic.
- Author
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Yang YL, Chen CC, Chin PW, Cheng CG, and Cheng CA
- Subjects
- Humans, Pandemics, Taiwan epidemiology, Public Health, COVID-19 epidemiology, Foodborne Diseases epidemiology, Foodborne Diseases microbiology, Foodborne Diseases prevention & control
- Abstract
Background and Objectives : The coronavirus disease 2019 (COVID-19) pandemic originated in Wuhan, China, in December 2019, the first case diagnosed since January 2020 in Taiwan. The study about the potential impact of the COVID-19 pandemic on event, location, food source, and pathogens of foodborne disease (FBD) is limited in Taiwan. Our aim in this study is to investigate FBD in the context of the COVID-19 pandemic. Materials and Methods : We collected publicly available annual summary data from the FBD dataset in the Taiwan Food and Drug Administration and Certifiable Disease on reported FBD in Taiwan from 2019 to 2020. We used logistic regression to evaluate changes in the occurrence or likelihood of FBD cases and Poisson regression to examine the relative risk (RR) between FBD and climate factors. Results : Similar events occurred in 2019 and 2020, but the total number of FBD cases decreased from 6935 in 2019 to 4920 in 2020. The places where FBD decreased were in schools, hospitals, outdoors, vendors, and exteriors. The top place in FBD shifted from schools to restaurants. The top food source for FBD has changed from boxed food to compound food. Bacillus cereus and Salmonella emerged as the top two observed bacterial pathogens causing FBD. The risk of FBD cases increased with a higher air temperature, with an RR of 1.055 (1.05-1.061, p < 0.001) every 1 °C. Conclusion : The incidence of FBD decreased significantly during the COVID-19 pandemic in Taiwan. This decline may be attributed to protective measures implemented to control the spread of the virus. This shift in locations could be influenced by changes in public behavior, regulations, or other external factors. The study emphasizes the importance of understanding the sources and effectiveness of severe infection prevention policies. The government can use these findings to formulate evidence-based policies aimed at reducing FBD cases and promoting public health. Consumers can reduce the risk of FBD by following safe food handling and preparation recommendations.
- Published
- 2024
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48. Raman spectroscopy-based microfluidic platforms: A promising tool for detection of foodborne pathogens in food products.
- Author
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Jayan H, Yin L, Xue S, Zou X, and Guo Z
- Subjects
- Humans, Spectrum Analysis, Raman, Food Microbiology, Food Safety, Microfluidics, Foodborne Diseases prevention & control
- Abstract
Rapid and sensitive detection of foodborne pathogens in food products is paramount for ensuring food safety and public health. In the ongoing effort to tackle this issue, detection methods are continually researched and upgraded to achieve rapidity, sensitivity, portability, and cost-effectiveness. This review addresses the critical need for improved technique by focusing on Raman spectroscopy-based microfluidic platforms, which have shown potential in revolutionizing the field of foodborne pathogen analysis offering point-of-care diagnosis and multiplex detection. The key problem lies in the persistent threat of compromised food quality and public health due to inadequate pathogen detection. The review elucidates the various trapping strategies employed in a microfluidic platform, including optical trapping, electrical trapping, mechanical trapping, and acoustic trapping for the capture of microbial cells. Subsequently, the review delves into the key aspects of the application of microbial detection in food products, highlighting recent advances and challenges in the field. The integrated technique allows point-of-care application assessment, which is an attractive quality for in-line and real-time detection of foodborne pathogens. However, the application of the technique in food products is limited and requires further research to combat the complexity of the food matrix, reduced costs of production, and ensure real-time use for diverse pathogens. Ultimately, this review aims to propel advancements in microbial detection, thus promoting enhanced food safety through state-of-the-art technologies., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
- Full Text
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49. Antibacterial activity of flavonoid extracts from Enteromorpha intestinalis and Caulerpa prolifera against multidrug-resistant foodborne bacterial isolates.
- Author
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Alghazeer RO, Azwai SM, Elmansori AA, Alzintani KM, Elghmasi S, Alnajjar AY, Gammoudi FT, Sidati M, Hawisa ST, Garbaj AM, and Eldaghayes IM
- Subjects
- Ulva chemistry, Plant Extracts pharmacology, Plant Extracts chemistry, Foodborne Diseases microbiology, Foodborne Diseases prevention & control, Foodborne Diseases veterinary, Animals, Flavonoids pharmacology, Flavonoids chemistry, Anti-Bacterial Agents pharmacology, Microbial Sensitivity Tests, Drug Resistance, Multiple, Bacterial drug effects, Caulerpa chemistry
- Abstract
Background: Food poisoning caused by bacterial agents is a worldwide problem, usually accompanied by unpleasant symptoms and may be severe leading to death. Natural compounds from marine algae namely flavonoids may play a role in the remedy of this condition., Aim: This research aims to assess the potency of flavonoids extracted from Enteromorpha intestinalis and Caulerpa prolifera as antibacterial agents., Methods: Enteromorpha intestinalis was collected from Western Libyan Coast and C. prolifera was collected from Farwa Island. The antimicrobial activity and determination of minimum inhibitory concentration of algal flavonoid-containing extracts was performed in vitro against some positive and negative Gram bacteria., Results: Crude extract containing flavonoids from E. intestinalis was more effective than C. prolifera extract against Staphylococcus aureus with antimicrobial essay (25-28 + 1 and 14.5-37.5 + 0.5-1.5), MIC (50 and 50-250 µg/ml), MBC (75 and 75-250 µg/ml). In Bacillus cereus, the antimicrobial assay (19-24.5 + 0.5-1.5: 24 + 1), MIC (50-250 + 100 µg/ml), and MBC (250 and 125 µg/ml). On the other hand, flavonoids containing extract from C. prolifera were more effective than E. intestinalis against Enterohemorrhagic Escherichia coli O157 EHEC O157 (25-28 + 1: 14-18.5 + 0.5-1.5), MIC (100-250:100-500 µg/ml), and MBC (150-250 and 250-500 µg/ml). Salmonella enterica qualitatively combat by flavonoid from E. intestinalis (13.5-14 + 0.5-1: 10.5-13.5 + 0.5-1.5), MIC (100-250: 250 µg/ml), and MBC (100-250: 250 µg/ml). Flavonoids from C. prolifera (4 strains: 2 strains) were effective against S. enterica. Crude flavonoids from both algae were not effective against Bacillus pumilus., Conclusion: Data from this study could conclude that flavonoid extracts from E. intestinalis and C. prolifera could be used against foodborne bacterial agents., Competing Interests: All authors declare that there is no conflict of interest.
- Published
- 2024
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50. Understanding the role of household hygiene practices and foodborne disease risks in child stunting: a UKRI GCRF Action Against Stunting Hub protocol paper.
- Author
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Dominguez-Salas P, Waddington HS, Grace D, Bosire C, Moodley A, Kulkarni B, Dasi T, Banjara SK, Kumar RN, Fahmida U, Htet MK, Sudibya ARP, Faye B, Tine RC, Heffernan C, Saxena D, Dreibelbis R, and Häsler B
- Subjects
- Infant, Child, Pregnancy, Animals, Humans, Female, Growth Disorders epidemiology, Growth Disorders etiology, Growth Disorders prevention & control, Hygiene, Surveys and Questionnaires, Observational Studies as Topic, Drinking Water, Foodborne Diseases epidemiology, Foodborne Diseases prevention & control
- Abstract
Introduction: Environmental hygiene and food safety are important determinants of child stunting. This research aims to explore the relationship between child stunting and household hygiene practices and behaviours, including the availability of water, sanitation and hygiene (WASH) facilities; the use of safe food and good quality drinking water (especially when used for complementary feeding); hygienic practices in food transport, storage and preparation and the control of cross-contamination from animals, their produce and waste., Methods and Analysis: This study is part of a wider observational study which aims to investigate the interdisciplinary factors contributing to child stunting using a 'whole child' paradigm. The observational study recruits women during pregnancy in Hyderabad, India, Lombok, Indonesia and Kaffrine, Senegal, and dyads (ie, 500 mother-infant pairs per country) are followed longitudinally up to 24 months after birth. Within the interdisciplinary niche, the study here has developed tools to investigate the potential exposure pathways to environmental pathogen contamination of foods and water. Holistic WASH and food safety data collection tools have been developed to explore exposure pathways at the household level, including: (1) survey questionnaires; (2) spot-checks; (3) biological sampling of drinking water, food and domestic surfaces and (4) direct observation. An integrated analytical approach will be used to triangulate the evidence in order to examine the relationships between child stunting, WASH and food safety behaviours., Ethics and Dissemination: Ethical approval of the study was granted by the ethics committee of the LSHTM, RVC, ILRI, ICMR, IIPHG, SEAMEO-RECFON, University of Cheikh Anta Diop. Findings of the study will be disseminated through publication in peer-reviewed journals, relevant international conferences, public engagement events, and policy-maker and stakeholder events., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2024. Re-use permitted under CC BY. Published by BMJ.)
- Published
- 2024
- Full Text
- View/download PDF
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