36 results on '"Ferreira FD"'
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2. Nutritional composition and bioactivity of Umbilicus rupestris (Salisb.) Dandy: An underexploited edible wild plant.
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Harumi Iyda J, Fernandes Â, Calhelha RC, Alves MJ, Ferreira FD, Barros L, Amaral JS, and Ferreira ICFR
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- Antioxidants analysis, Flavonoids analysis, Hydroxybenzoates analysis, Plant Extracts analysis, Plant Extracts chemistry, Plant Leaves chemistry, Polyphenols analysis, Portugal, Tocopherols analysis, Magnoliopsida chemistry, Nutritive Value, Plants, Edible chemistry
- Abstract
The inclusion of edible wild plants in human diet has been receiving an increasing attention, as they represent an easily accessible source of nutrients, vitamins and antioxidants. In this work, the leaves of Umbelicus rupestris (Salisb.) Dandy, an edible species for which only scarce data is available in literature, were thoroughly evaluated for its nutritional profile, chemical characterization and bioactive properties. Being considered a succulent plant, the leaves revealed a very high content of moisture, with several beneficial compounds, including omega-3 polyunsaturated fatty acids, tocopherols and different polyphenols. A total of twelve flavonoids, three phenolic acids and one phenylpropanoid glucoside were identified in the decoction and/or hydroethanolic extracts, with most of them being described for the first time in this plant. Both extracts showed antioxidant activity and potential to inhibit some of the assayed bacteria, while not presenting cytotoxic effects on a non-tumour primary cell culture., (Copyright © 2019 Elsevier Ltd. All rights reserved.)
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- 2019
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3. Chemical composition and bioactive properties of the wild edible plant Raphanus raphanistrum L.
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Iyda JH, Fernandes Â, Ferreira FD, Alves MJ, Pires TCSP, Barros L, Amaral JS, and Ferreira ICFR
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- Anti-Bacterial Agents analysis, Anti-Bacterial Agents pharmacology, Antioxidants analysis, Food Analysis, Gram-Negative Bacteria drug effects, Gram-Negative Bacteria metabolism, Gram-Positive Bacteria drug effects, Gram-Positive Bacteria metabolism, Kaempferols analysis, Nutritive Value, Phenols analysis, Phenols pharmacology, Plant Extracts analysis, Plant Extracts pharmacology, Thiobarbituric Acid Reactive Substances analysis, Plants, Edible chemistry, Raphanus chemistry
- Abstract
Recently, there has been an interest regarding the consumption of wild edible plants in modern diets. However, there is still scarce information about several wild vegetables traditionally consumed. Therefore, this work aims on documenting the nutritional and chemical composition of wild radish (Raphanus raphanistrum L.), as well as its bioactive potential. Results showed that wild radish is a potential source of beneficial compounds, including vitamin E, polyunsaturated fatty acid (particularly α-linolenic acid) and different phenolic compounds, in which fourteen phenolics were identified, with kaempferol-3,7-O-di-rhamnoside being the most abundant. The bioactive potential was exploited using hydroethanolic and decoction extracts. Both proved to inhibit several Gram-positive and Gram-negative bacteria and revealed antioxidant activity, while cytotoxicity against non-tumor cell was not observed. In general, results evidence the interest in recovering the use of this wild vegetable as part of a varied diet, which can bring several health benefits., (Copyright © 2018 Elsevier Ltd. All rights reserved.)
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- 2019
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4. Diminished cholesterol efflux mediated by HDL and coronary artery disease in young male anabolic androgenic steroid users.
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Souza FR, Dos Santos MR, Porello RA, Fonseca GWPD, Sayegh ALC, Lima TP, Ferreira FD, Oliveira TF, Yonamine M, Takayama L, Pereira RMR, Negrão CE, Passarelli M, Rochitte CE, and Alves MJNN
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- Adolescent, Adult, Anabolic Agents adverse effects, Biomarkers blood, Computed Tomography Angiography, Coronary Angiography, Coronary Artery Disease chemically induced, Coronary Artery Disease diagnosis, Cross-Sectional Studies, Follow-Up Studies, Healthy Volunteers, Humans, Lipoproteins, HDL drug effects, Male, Middle Aged, Retrospective Studies, Young Adult, Cholesterol blood, Coronary Artery Disease blood, Lipoproteins, HDL blood, Testosterone Congeners adverse effects
- Abstract
Background and Aims: Anabolic androgenic steroids (AAS) have been associated with coronary artery disease (CAD). AAS abuse leads to a remarkable decrease in high-density lipoprotein (HDL) plasma concentration, which could be a key factor in the atherosclerotic process. Moreover, not only the concentration of HDL, but also its functionality, plays a pivotal role in CAD. We tested the functionality of HDL by cholesterol efflux and antioxidant capacity. We also evaluated the prevalence of CAD in AAS users., Methods: Twenty strength-trained AAS users (AASU) age 29 ± 5 yr, 20 age-matched strength-trained AAS nonusers (AASNU), and 10 sedentary controls (SC) were enrolled in this cross-sectional study. Functionality of HDL was evaluated by
14 C-cholesterol efflux and the ability of HDL in inhibiting LDL oxidation. Coronary artery was evaluated with coronary computed tomography angiography., Results: Cholesterol efflux was lower in AASU compared with AASNU and SC (20 vs. 23 vs. 24%, respectively, p < 0.001). However, the lag time for LDL oxidation was higher in AASU compared with AASNU and SC (41 vs 13 vs 11 min, respectively, p < 0.001). We found at least 2 coronary arteries with plaques in 25% of AASU. None of the AASNU and SC had plaques. The time of AAS use was negatively associated with cholesterol efflux., Conclusions: This study indicates that AAS abuse impairs the cholesterol efflux mediated by HDL. Long-term AAS use seems to be correlated with lower cholesterol efflux and early subclinical CAD in this population., (Copyright © 2019. Published by Elsevier B.V.)- Published
- 2019
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5. Biological Safety Studies and Simultaneous Determination of Linagliptin and Synthetic Impurities by LC-PDA.
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Heleno Ferreira RB, Duarte JA, Ferreira FD, de Oliveira LFS, Machado MM, Malesuik MD, Paula FR, Steppe M, Schapoval EES, and Paim CS
- Abstract
A stability-indicating LC method was developed for quantification of linagliptin (LGT) and three synthetic impurities. The method utilizes a Thermo Scientific® RP-8 column (100 mm × 4.6 mm; 5 μ m) with the PDA detector for quantitation of impurities. A mixture of 0.1% formic acid with pH 3.5 (A) and acetonitrile (B) was used as the mobile phase at a flow rate of 0.6 mL·min
-1 with gradient elution. The percentage of mobile phase B increases from 30% to 70% over 5 min and decreases from 70% to 30% between 5 and 8 min. The method was validated according to International Council for Harmonization (ICH) guidelines. The LOD values obtained were 0.0171 μ g·mL-1 and 0.015 μ g·mL-1 for LGT and impurities, respectively. The LOQ values were 0.06 μ g·mL-1 for LGT and impurities. In all cases, the correlation coefficients of LGT and impurities were >0.999, showing the linearity of the method. The % recovery of the LGT and added impurity were in the range of 92.92-99.79%. The precision of the method showed values less than 1.47% for LGT and less than 4.63% for impurities. The robustness was also demonstrated by small modifications in the chromatographic conditions. The selectivity was evidenced because the degradation products formed in stress conditions did not interfere in the determination of LGT and impurities. Toxicity prediction studies suggested toxicity potential of the impurities, which was confirmed using biological safety studies in vitro.- Published
- 2019
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6. Effect of Zingiber officinale Roscoe essential oil in fungus control and deoxynivalenol production of Fusarium graminearum Schwabe in vitro.
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Ferreira FMD, Hirooka EY, Ferreira FD, Silva MV, Mossini SAG, and Machinski M Jr
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- Antifungal Agents chemistry, Antifungal Agents isolation & purification, Microbial Sensitivity Tests, Oils, Volatile chemistry, Oils, Volatile isolation & purification, Trichothecenes chemistry, Antifungal Agents pharmacology, Fusarium chemistry, Fusarium drug effects, Zingiber officinale chemistry, Oils, Volatile pharmacology, Trichothecenes biosynthesis
- Abstract
Members of the Fusarium genus are capable of contaminating agricultural commodities, compromising the quality of maize and other grains, which leads to severe quality and yield losses. Contamination with mycotoxins is also a concern. Essential oils are possible alternatives to the use of synthetic pesticides for control of fungal contamination, as many have antifungal and anti-mycotoxigenic properties and are innocuous to human health. They also do not cause any sort of microbial resistance and do not promote environmental pollution. The aim of this study was to evaluate the antifungal and anti-mycotoxigenic effects of Zingiber officinale Roscoe essential oil (GEO) upon Fusarium graminearum Schwabe in vitro. The essential oil was extracted by hydrodistillation and analysed by GC/MS. Antifungal and anti-mycotoxigenic activities were assessed by HPLC/UV by quantifying ergosterol and deoxynivalenol (DON), respectively. Results indicated that GEO inhibited ergosterol production at a concentration of 1000 µg/mL and DON production at a concentration of 500 µg/mL, evidencing that the anti-mycotoxigenic effect is independent of the antifungal effect due to its probable direct action upon toxin biosynthesis.
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- 2018
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7. Occurrence and risk assessment of population exposed to deoxynivalenol in foods derived from wheat flour in Brazil.
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Silva MV, Pante GC, Romoli JCZ, de Souza APM, Rocha GHOD, Ferreira FD, Feijó ALR, Moscardi SMP, de Paula KR, Bando E, Nerilo SB, and Machinski M Jr
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- Adolescent, Adult, Aged, Brazil, Child, Chromatography, High Pressure Liquid, Female, Humans, Male, Middle Aged, Risk Assessment, Young Adult, Flour analysis, Food Analysis, Food Contamination analysis, Trichothecenes analysis, Triticum chemistry
- Abstract
Deoxynivalenol (DON) is the most important of the trichothecenes in terms of amounts and occurrence in wheat. This compound was shown to be associated with a glomerulonephropathy involving an increase of immunoglobulin A in humans. This study assessed the occurrence of DON in wheat flour and the exposure of Brazilian teenagers, adults and elderly to this mycotoxin due to intake of wheat flour-based products. DON extraction in wheat flour was carried out by solid phase extraction and the quantification was performed by ultra-high proficiency liquid chromatography with diode-array detection. A total of 77.9% of all samples were positive for DON, with concentrations ranging from 73.50 to 2794.63 µg kg
-1 . The intake was calculated for the average and 90th percentile of the contamination levels of DON in foods based-wheat for teenagers, adults and elderly in Brazil, and compared with the provisional maximum tolerable daily intakes (PMTDI). Females of all age groups were exposed to DON at higher levels when compared to males in regard of consumption of breads and pastas. Teenagers were the main consumers of foods derived from wheat flour, with maximum probable daily intakes of 1.28 and 1.20 µg kg-1 b.w. day-1 for females and males, respectively. This population is at an increased risk of exposure to DON due to consumption of wheat flour-based foods in Brazil.- Published
- 2018
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8. Corrigendum to "Dietary interesterified fat enriched with palmitic acid induces atherosclerosis by impairing macrophage cholesterol efflux and eliciting inflammation" [J Nutr Biochem 32C (2016) 91-100].
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Afonso MS, Lavrador MS, Koike MK, Cintra DE, Ferreira FD, Nunes VS, Castilho G, Gioielli LA, Bombo RP, Catanozi S, Caldini EG, Damaceno-Rodrigues NR, Passarelli M, Nakandakare ER, and Lottenberg AM
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- 2016
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9. Dietary interesterified fat enriched with palmitic acid induces atherosclerosis by impairing macrophage cholesterol efflux and eliciting inflammation.
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Afonso MS, Lavrador MS, Koike MK, Cintra DE, Ferreira FD, Nunes VS, Castilho G, Gioielli LA, Paula Bombo R, Catanozi S, Caldini EG, Damaceno-Rodrigues NR, Passarelli M, Nakandakare ER, and Lottenberg AM
- Subjects
- Animals, Aorta immunology, Aorta metabolism, Aorta pathology, Atherosclerosis immunology, Atherosclerosis metabolism, Atherosclerosis pathology, Biomarkers blood, Biomarkers metabolism, Cholesterol blood, Cytokines blood, Cytokines metabolism, Endothelium, Vascular immunology, Endothelium, Vascular pathology, Esterification, Gene Expression Regulation, Developmental, Macrophage Activation, Macrophages immunology, Macrophages pathology, Male, Mice, Inbred C57BL, Mice, Knockout, Palmitic Acid chemistry, Random Allocation, Receptors, LDL genetics, Receptors, LDL metabolism, Stearic Acids adverse effects, Stearic Acids chemistry, Trans Fatty Acids adverse effects, Trans Fatty Acids chemistry, Triglycerides chemistry, Weaning, Atherosclerosis etiology, Cholesterol metabolism, Diet, High-Fat adverse effects, Endothelium, Vascular metabolism, Macrophages metabolism, Palmitic Acid adverse effects, Triglycerides adverse effects
- Abstract
Interesterified fats are currently being used to replace trans fatty acids. However, their impact on biological pathways involved in the atherosclerosis development was not investigated. Weaning male LDLr-KO mice were fed for 16weeks on a high-fat diet (40% energy as fat) containing polyunsaturated (PUFA), TRANS, palmitic (PALM), palmitic interesterified (PALM INTER), stearic (STEAR) or stearic interesterified (STEAR INTER). Plasma lipids, lipoprotein profile, arterial lesion area, macrophage infiltration, collagen content and inflammatory response modulation were determined. Macrophage cholesterol efflux and the arterial expression of cholesterol uptake and efflux receptors were also performed. The interesterification process did not alter plasma lipid concentrations. Although PALM INTER did not increase plasma cholesterol concentration as much as TRANS, the cholesterol enrichment in the LDL particle was similar in both groups. Moreover, PALM INTER induced the highest IL-1β, MCP-1 and IL-6 secretion from peritoneal macrophages as compared to others. This inflammatory response elicited by PALM INTER was confirmed in arterial wall, as compared to PALM. These deleterious effects of PALM INTER culminate in higher atherosclerotic lesion, macrophage infiltration and collagen content than PALM, STEAR, STEAR INTER and PUFA. These events can partially be attributed to a macrophage cholesterol accumulation, promoted by apoAI and HDL2-mediated cholesterol efflux impairment and increased Olr-1 and decreased Abca1 and Nr1h3 expressions in the arterial wall. Interesterified fats containing palmitic acid induce atherosclerosis development by promoting cholesterol accumulation in LDL particles and macrophagic cells, activating the inflammatory process in LDLr-KO mice., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2016
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10. Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro.
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do Amaral RJ, da Silva NP, Haddad NF, Lopes LS, Ferreira FD, Filho RB, Cappelletti PA, de Mello W, Cordeiro-Spinetti E, and Balduino A
- Abstract
There are promising results in the use of platelet-rich plasma (PRP) for musculoskeletal tissue repair. However, the variability in the methodology for its obtaining may cause different and opposing findings in the literature. Particularly, the choice of the anticoagulant is the first definition to be made. In this work, blood was collected with sodium citrate (SC), ethylenediaminetetraacetic acid (EDTA), or anticoagulant citrate dextrose (ACD) solution A, as anticoagulants, prior to PRP obtaining. Hematological analysis and growth factors release quantification were performed, and the effects on mesenchymal stromal cell (MSC) culture, such as cytotoxicity and cell proliferation (evaluated by MTT method) and gene expression, were evaluated. The use of EDTA resulted in higher platelet yield in whole blood; however, it induced an increase in the mean platelet volume (MPV) following the blood centrifugation steps for PRP obtaining. The use of SC and ACD resulted in higher induction of MSC proliferation. On the other hand, PRP obtained in SC presented the higher platelet recovery after the blood first centrifugation step and a minimal change in MSC gene expression. Therefore, we suggest the use of SC as the anticoagulant for PRP obtaining.
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- 2016
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11. Comparison between qualitative and real-time polymerase chain reaction to evaluate minimal residual disease in children with acute lymphoblastic leukemia.
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Paula FD, Elói-Santos SM, Xavier SG, Ganazza MA, Jotta PY, Yunes JA, Viana MB, and Assumpção JG
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Introduction: Minimal residual disease is an important independent prognostic factor that can identify poor responders among patients with acute lymphoblastic leukemia., Objective: The aim of this study was to analyze minimal residual disease using immunoglobulin (Ig) and T-cell receptor (TCR) gene rearrangements by conventional polymerase chain reaction followed by homo-heteroduplex analysis and to compare this with real-time polymerase chain reaction at the end of the induction period in children with acute lymphoblastic leukemia., Methods: Seventy-four patients diagnosed with acute lymphoblastic leukemia were enrolled. Minimal residual disease was evaluated by qualitative polymerase chain reaction in 57 and by both tests in 44. The Kaplan-Meier and multivariate Cox methods and the log-rank test were used for statistical analysis., Results: Nine patients (15.8%) were positive for minimal residual disease by qualitative polymerase chain reaction and 11 (25%) by real-time polymerase chain reaction considering a cut-off point of 1×10(-3) for precursor B-cell acute lymphoblastic leukemia and 1×10(-2) for T-cell acute lymphoblastic leukemia. Using the qualitative method, the 3.5-year leukemia-free survival was significantly higher in children negative for minimal residual disease compared to those with positive results (84.1%±5.6% versus 41.7%±17.3%, respectively; p-value=0.004). There was no significant association between leukemia-free survival and minimal residual disease by real-time polymerase chain reaction. Minimal residual disease by qualitative polymerase chain reaction was the only variable significantly correlated to leukemia-free survival., Conclusion: Given the difficulties in the implementation of minimal residual disease monitoring by real-time polymerase chain reaction in most treatment centers in Brazil, the qualitative polymerase chain reaction strategy may be a cost-effective alternative., (Copyright © 2015 Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular. Published by Elsevier Editora Ltda. All rights reserved.)
- Published
- 2015
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12. Optimization and validation method to evaluate the residues of β-lactams and tetracyclines in kidney tissue by UPLC-MS/MS.
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de Almeida MP, Rezende CP, Ferreira FD, de Souza LF, de Assis DC, de Figueiredo TC, de Oliveira Leite M, and de Vasconcelos Cançado S
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- Animals, Birds, Cattle, Chromatography, Liquid methods, Horses, Limit of Detection, Reproducibility of Results, Swine, Tandem Mass Spectrometry, Anti-Bacterial Agents analysis, Kidney chemistry, Tetracyclines analysis, beta-Lactams analysis
- Abstract
Methods are validated by a process that defines the analytical requirements and confirms that the investigated method is capable of performing consistently. A quantitative and confirmatory method for determining the presence of β-lactam and tetracycline multiresidues in avian, bovine, equine, and swine kidney tissues using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed, optimized, and validated. Analytes were extracted from the kidneys by a mixture of water and acetonitrile, and the extract was then purified with hexane and C18 (dispersive phase). The method was evaluated by the following parameters: linearity, matrix effect, specificity, decision limits (CCα), detection capability (CCβ), accuracy, precision, trueness, limits of detection (LOD), limits of quantification (LOQ), and robustness. The validated method presented a broad linear study range and significant matrix effect. The limit of detection (LOD) was defined from 2.5 to 25.0 µg kg(-1), and the limit of quantification (LOQ) was defined from 5.0 to 50.0 µg kg(-1) for individual analytes. The resultant recovery values ranged from 98.1% to 107.3% in repeatability conditions and from 95.2% to 106% under intralaboratory reproducibility conditions for the studied analytes. It was concluded that the performance parameters demonstrated total method adequacy for detecting and quantifying β-lactam and tetracycline residues in swine, equine, bovine, and avian kidneys., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2015
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13. Oxytetracycline, tetracycline, chlortetracycline and doxycycline in pasteurised cow's milk commercialised in Brazil.
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Prado CK, Ferreira FD, Bando E, and Machinski M Jr
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- Animals, Brazil, Cattle, Chlortetracycline analysis, Chromatography, High Pressure Liquid, Doxycycline analysis, Drug Residues analysis, Humans, Maximum Allowable Concentration, Oxytetracycline analysis, Pasteurization, Tetracycline analysis, Anti-Bacterial Agents analysis, Food Contamination analysis, Milk chemistry, Tetracyclines analysis
- Abstract
The aim of this study was to investigate the presence of tetracycline residues in pasteurised cow's milk using high-performance liquid chromatography coupled with UV/VIS detection to determine the exposure of Brazilian's population to antibiotic residues. One hundred samples collected from the State of Paraná, Brazil, were analysed. Three of these samples were contaminated at the following concentrations: 121.8 µg·kg(-1) for oxytetracycline, 93.5 µg·kg(-1) for tetracycline and 134.6 µg·kg(-1) for chlortetracycline (61.6 µg·kg(-1)) and doxycycline (73.0 µg·kg(-1)). The median tetracycline residue concentration found in the samples was 42.3 µg·kg(-1), and the estimated daily intake (EDI) was 0.05 µg Kg(-1) bw day(-1) in Brazil. These results demonstrate that the occurrence of tetracycline in Brazilian milk was low (3%) and only for 2% above the maximum residue limit, so the risk to the population from the presence of these residues in milk was low (<1% of the acceptable daily intake).
- Published
- 2015
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14. Effect of Zingiber officinale essential oil on Fusarium verticillioides and fumonisin production.
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Yamamoto-Ribeiro MM, Grespan R, Kohiyama CY, Ferreira FD, Mossini SA, Silva EL, Filho BA, Mikcha JM, and Machinski M Jr
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- Antifungal Agents chemistry, Fusarium growth & development, Fusarium metabolism, Microbial Sensitivity Tests, Oils, Volatile chemistry, Plant Oils chemistry, Antifungal Agents pharmacology, Fumonisins metabolism, Fusarium drug effects, Zingiber officinale chemistry, Oils, Volatile pharmacology, Plant Oils pharmacology
- Abstract
The antifungal activity of ginger essential oil (GEO; Zingiber officinale Roscoe) was evaluated against Fusarium verticillioides (Saccardo) Nirenberg. The minimum inhibitory concentration (MIC) of GEO was determined by micro-broth dilution. The effects of GEO on fumonisin and ergosterol production were evaluated at concentrations of 500-5000 μg/mL in liquid medium with a 5mm diameter mycelial disc of F. verticillioides. Gas chromatography-mass spectrometry showed that the predominant components of GEO were α-zingiberene (23.9%) and citral (21.7%). GEO exhibited inhibitory activity, with a MIC of 2500 μg/mL, and 4000 and 5000 μg/mL reduced ergosterol biosynthesis by 57% and 100%, respectively. The inhibitory effect on fumonisin B1 (FB1) and fumonisin B2 (FB2) production was significant at GEO concentrations of 4000 and 2000 μg/mL, respectively. Thus, the inhibition of fungal biomass and fumonisin production was dependent on the concentration of GEO. These results suggest that GEO was able to control the growth of F. verticillioides and subsequent fumonisin production., (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Published
- 2013
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15. Identification of Aspergillus flavus isolates as potential biocontrol agents of aflatoxin contamination in crops.
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Rosada LJ, Sant'anna JR, Franco CC, Esquissato GN, Santos PA, Yajima JP, Ferreira FD, Machinski M, Corrêa B, and Castro-Prado MA
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- Aflatoxins biosynthesis, Aflatoxins genetics, Arachis microbiology, Aspergillus flavus genetics, Aspergillus flavus metabolism, Genetic Variation, Recombination, Genetic, Zea mays microbiology, Aflatoxins analysis, Aspergillus flavus physiology, Food Contamination prevention & control, Food Microbiology
- Abstract
Aspergillus flavus, a haploid organism found worldwide in a variety of crops, including maize, cottonseed, almond, pistachio, and peanut, causes substantial and recurrent worldwide economic liabilities. This filamentous fungus produces aflatoxins (AFLs) B1 and B2, which are among the most carcinogenic compounds from nature, acutely hepatotoxic and immunosuppressive. Recent efforts to reduce AFL contamination in crops have focused on the use of nonaflatoxigenic A. flavus strains as biological control agents. Such agents are applied to soil to competitively exclude native AFL strains from crops and thereby reduce AFL contamination. Because the possibility of genetic recombination in A. flavus could influence the stability of biocontrol strains with the production of novel AFL phenotypes, this article assesses the diversity of vegetative compatibility reactions in isolates of A. flavus to identify heterokaryon self-incompatible (HSI) strains among nonaflatoxigenic isolates, which would be used as biological controls of AFL contamination in crops. Nitrate nonutilizing (nit) mutants were recovered from 25 A. flavus isolates, and based on vegetative complementation between nit mutants and on the microscopic examination of the number of hyphal fusions, five nonaflatoxigenic (6, 7, 9 to 11) and two nontoxigenic (8 and 12) isolates of A. flavus were phenotypically characterized as HSI. Because the number of hyphal fusions is reduced in HSI strains, impairing both heterokaryon formation and the genetic exchanges with aflatoxigenic strains, the HSI isolates characterized here, especially isolates 8 and 12, are potential agents for reducing AFL contamination in crops.
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- 2013
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16. Fusarium species and fumonisins associated with maize kernels produced in Rio Grande do Sul State for the 2008/09 and 2009/10 growing seasons.
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Stumpf R, Dos Santos J, Gomes LB, Silva CN, Tessmann DJ, Ferreira FD, Machinski M Jr, and Del Ponte EM
- Abstract
Ear rots caused by Fusarium spp. are among the main fungal diseases that contribute to poor quality and the contamination of maize grains with mycotoxins. This study aimed to determine the visual incidence of fungal-damaged kernels (FDKs), the incidence of two main Gibberella (a teleomorph of Fusarium) complexes (G. fujikuroi and G. zeae) associated with maize using a seed health blotter test, and the fumonisin levels, using high performance liquid chromatography, in samples of maize grains grown across 23 municipalities during the 2008/09 and 2009/10 growing seasons. Additionally, 104 strains that were representative of all of the analysed samples were identified to species using PCR assays. The mean FDK was seven per cent, and only six of the samples had levels greater than six per cent. Fusarium spp. of the G. fujikuroi complex were present in 96% of the samples, and G. zeae was present in 18% of the samples (5/27). The mean incidence of G. fujikuroi was 58%, and the incidence of G. zeae varied from 2 to 6%. FB1 was found in 58.6%, FB2 in 37.9%, and both toxins in 37.9% of the samples. The FB1 and FB2 levels were below the quantification limits for 41.3% of the samples, and the mean FB1 levels (0.66 μg/g) were higher than the mean FB2 levels (0.42 μg/g). The PCR identification separated the 104 isolates into three of the G. fujikuroi complex: F. verticillioides (76%), F. subglutinans (4%) and F. proliferatum (2%); and G. zeae (anamorph = F. graminearum) (18%). Our results confirmed the dominance of F. verticillioides, similar to other regions of Brazil, but they differed due to the relatively higher incidence of F. graminearum. Total fumonisin levels were below the maximum limit determined by current Brazilian regulations.
- Published
- 2013
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17. Inhibitory effect of the essential oil of Curcuma longa L. and curcumin on aflatoxin production by Aspergillus flavus Link.
- Author
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Ferreira FD, Kemmelmeier C, Arrotéia CC, da Costa CL, Mallmann CA, Janeiro V, Ferreira FM, Mossini SA, Silva EL, and Machinski M Jr
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- Antifungal Agents chemistry, Antioxidants chemistry, Antioxidants pharmacology, Aspergillus flavus metabolism, Down-Regulation drug effects, Oils, Volatile chemistry, Plant Oils chemistry, Aflatoxins biosynthesis, Antifungal Agents pharmacology, Aspergillus flavus drug effects, Curcuma chemistry, Oils, Volatile pharmacology, Plant Oils pharmacology
- Abstract
Aflatoxins are highly toxic, mutagenic, teratogenic and carcinogenic mycotoxins. Consumption of aflatoxin-contaminated food and commodities poses serious hazards to the health of humans and animals. Turmeric, Curcuma longa L., is a native plant of Southeast Asia and has antimicrobial, antioxidant and antifungal properties. This paper reports the antiaflatoxigenic activities of the essential oil of C. longa and curcumin. The medium tests were prepared with the oil of C. longa, and the curcumin standard at concentrations varied from 0.01% to 5.0%. All doses of the essential oil of the plant and the curcumin standard interfered with mycotoxin production. Both the essential oil and curcumin significantly inhibited the production of aflatoxins; the 0.5% level had a greater than 96% inhibitory effect. The levels of aflatoxin B(1) (AFB(1)) production were 1.0 and 42.7 μg/mL, respectively, for the samples treated with the essential oil of C. longa L. and curcumin at a concentration of 0.5%., (Copyright © 2012 Elsevier Ltd. All rights reserved.)
- Published
- 2013
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18. Gene rearrangement study for minimal residual disease monitoring in children with acute lymphocytic leukemia.
- Author
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Assumpção JG, Paula FD, Xavier SG, Murao M, de Aguirre JC Neto, Dutra AP, Lima ER, de Oliveira BM, and Viana MB
- Abstract
Objective: To detect markers for minimal residual disease monitoring based on conventional polymerase chain reaction for immunoglobulin, T-cell receptor rearrangements and the Sil-Tal1 deletion in patients with acute lymphocytic leukemia., Methods: Fifty-nine children with acute lymphocytic leukemia from three institutions in Minas Gerais, Brazil, were prospectively studied. Clonal rearrangements were detected by polymerase chain reaction followed by homo/heteroduplex clonality analysis in DNA samples from diagnostic bone marrow. Follow-up samples were collected on Days 14 and 28-35 of the induction phase. The Kaplan-Meier and multivariate Cox methods were used for survival analysis., Results: Immunoglobulin/T-cell receptor rearrangements were not detected in 5/55 children screened (9.0%). For precursor-B acute lymphocytic leukemia, the most frequent rearrangement was IgH (72.7%), then TCRG (61.4%), and TCRD and IgK (47.7%); for T-acute lymphocytic leukemia, TCRG (80.0%), and TCRD and Sil-Tal deletion (20.0%) were the most common. Minimal residual disease was detected in 35% of the cases on Day 14 and in 22.5% on Day 28-35. Minimal residual disease on Day 28-35, T-acute lymphocytic leukemia, and leukocyte count above 50 x 10(9)/L at diagnosis were bad prognostic factors for leukemia-free survival in univariate analysis. Relapse risk for minimal residual disease positive relative to minimal residual disease negative children was 8.5 times higher (95% confidence interval: 1.02-70.7)., Conclusion: Immunoglobulin/T-cell receptor rearrangement frequencies were similar to those reported before. Minimal residual disease is an independent prognostic factor for leukemia-free survival, even when based on a non-quantitative technique, but longer follow-ups are needed.
- Published
- 2013
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19. Omega-6 polyunsaturated fatty acids prevent atherosclerosis development in LDLr-KO mice, in spite of displaying a pro-inflammatory profile similar to trans fatty acids.
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Machado RM, Nakandakare ER, Quintao EC, Cazita PM, Koike MK, Nunes VS, Ferreira FD, Afonso MS, Bombo RP, Machado-Lima A, Soriano FG, Catanozi S, and Lottenberg AM
- Subjects
- ATP Binding Cassette Transporter 1, ATP-Binding Cassette Transporters biosynthesis, Animals, Aorta metabolism, Atherosclerosis metabolism, Atherosclerosis pathology, Cholesterol blood, Collagen metabolism, Diet, High-Fat, Dietary Fats, Unsaturated metabolism, Dietary Fats, Unsaturated therapeutic use, Inflammation metabolism, Inflammation prevention & control, Interleukin-10 metabolism, Interleukin-6 metabolism, Macrophages physiology, Male, Mice, Mice, Knockout, Receptors, LDL deficiency, Trans Fatty Acids pharmacology, Tumor Necrosis Factor-alpha metabolism, Atherosclerosis prevention & control, Fatty Acids, Omega-6 therapeutic use
- Abstract
The development of atherosclerosis and the inflammatory response were investigated in LDLr-KO mice on three high-fat diets (40% energy as fat) for 16 weeks: trans (TRANS), saturated (SAFA) or ω-6 polyunsaturated (PUFA) fats. The following parameters were measured: plasma lipids, aortic root total cholesterol (TC), lesion area (Oil Red-O), ABCA1 content and macrophage infiltration (immunohistochemistry), collagen content (Picrosirius-red) and co-localization of ABCA1 and macrophage (confocal microscopy) besides the plasma inflammatory markers (IL-6, TNF-α) and the macrophage inflammatory response to lipopolysaccharide from Escherichia coli (LPS). As expected, plasma TC and TG concentrations were lower on the PUFA diet than on TRANS or SAFA diets. Aortic intima macrophage infiltration, ABCA1 content, and lesion area on PUFA group were lower compared to TRANS and SAFA groups. Macrophages and ABCA1 markers did not co-localize in the atherosclerotic plaque, suggesting that different cell types were responsible for the ABCA1 expression in plaques. Compared to PUFA, TRANS and SAFA presented higher collagen content and necrotic cores in atherosclerotic plaques. In the artery wall, TC was lower on PUFA compared to TRANS group; free cholesterol was lower on PUFA compared to TRANS and SAFA; cholesteryl ester concentration did not vary amongst the groups. Plasma TNF-α concentration on PUFA and TRANS-fed mice was higher compared to SAFA. No difference was observed in IL-6 concentration amongst groups. Regarding the macrophage inflammatory response to LPS, TRANS and PUFA presented higher culture medium concentrations of IL-6 and TNF-α as compared to SAFA. The PUFA group showed the lowest amount of the anti-inflammatory marker IL-10 compared to TRANS and SAFA groups. In conclusion, PUFA intake prevented atherogenesis, even in a pro-inflammatory condition., (Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2012
- Full Text
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20. Aerobic exercise improves reverse cholesterol transport in cholesteryl ester transfer protein transgenic mice.
- Author
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Rocco DD, Okuda LS, Pinto RS, Ferreira FD, Kubo SK, Nakandakare ER, Quintão EC, Catanozi S, and Passarelli M
- Subjects
- ATP Binding Cassette Transporter 1, ATP-Binding Cassette Transporters metabolism, Animals, Apolipoprotein A-I metabolism, Atherosclerosis metabolism, Biological Transport, Cholesterol blood, Cholesterol metabolism, Cholesterol 7-alpha-Hydroxylase metabolism, Cholesterol Ester Transfer Proteins genetics, Humans, Lipids blood, Lipoproteins, HDL blood, Lipoproteins, HDL metabolism, Lipoproteins, LDL blood, Liver chemistry, Macrophages chemistry, Macrophages metabolism, Male, Mice, Mice, Transgenic, Receptors, LDL metabolism, CD36 Antigens metabolism, Cholesterol Ester Transfer Proteins metabolism, Lipids analysis, Lipoproteins metabolism, Liver metabolism, Physical Conditioning, Animal physiology
- Abstract
We analyzed the effect of a 6-week aerobic exercise training program on the in vivo macrophage reverse cholesterol transport (RCT) in human cholesteryl ester transfer protein (CETP) transgenic (CETP-tg) mice. Male CETP-tg mice were randomly assigned to a sedentary group or a carefully supervised exercise training group (treadmill 15 m/min, 30 min sessions, five sessions per week). The levels of plasma lipids were determined by enzymatic methods, and the lipoprotein profile was determined by fast protein liquid chromatography (FPLC). CETP activity was determined by measuring the transfer rate of ¹⁴C-cholesterol from HDL to apo-B containing lipoproteins, using plasma from CETP-tg mice as a source of CETP. The reverse cholesterol transport was determined in vivo by measuring the [³H]-cholesterol recovery in plasma and feces (24 and 48 h) and in the liver (48 h) following a peritoneal injection of [³H]-cholesterol labeled J774-macrophages into both sedentary and exercise trained mice. The protein levels of liver receptors were determined by immunoblot, and the mRNA levels for liver enzymes were measured using RT-PCR. Exercise training did not significantly affect the levels of plasma lipids or CETP activity. The HDL fraction assessed by FPLC was higher in exercise-trained compared to sedentary mice. In comparison to the sedentary group, a greater recovery of [³H]-cholesterol from the injected macrophages was found in the plasma, liver and feces of exercise-trained animals. The latter occurred even with a reduction in the liver CYP7A1 mRNA level in exercised trained animals. Exercise training increased the liver LDL receptor and ABCA-1 protein levels, although the SR-BI protein content was unchanged. The RCT benefit in CETP-tg mice elicited by exercise training helps to elucidate the role of exercise in the prevention of atherosclerosis in humans.
- Published
- 2011
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21. Optical fiber biosensor coupled to chromatographic separation for screening of dopamine, norepinephrine and epinephrine in human urine and plasma.
- Author
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Silva LI, Ferreira FD, Freitas AC, Rocha-Santos TA, and Duarte AC
- Subjects
- Analysis of Variance, Biosensing Techniques instrumentation, Calibration, Dopamine blood, Dopamine urine, Electrochemistry, Epinephrine blood, Epinephrine urine, Humans, Norepinephrine blood, Norepinephrine urine, Polystyrenes chemistry, Biosensing Techniques methods, Chromatography, High Pressure Liquid methods, Dopamine isolation & purification, Epinephrine isolation & purification, Norepinephrine isolation & purification, Optical Fibers
- Abstract
An optical fiber biosensor has been developed for the determination of catecholamines (dopamine, norepinephrine and epinephrine) based on the recognition capacity of the enzyme laccase. In this study, a glass tube constituted by a fused silica fiber coated with a film of polystyrene/divinylbenzene resin (PS/DVB) was used for catecholamines separation. Firstly, the analyzer was tested for calibration and its analytical performance for catecholamines detection was compared with a classical analytical method, namely high performance liquid chromatography-electrochemical detector (HPLC-ED). The developed analytical device shows a high potential for catecholamines quantification with a detection limit of 2.1, 2.6 and 3.4 pg mL(-1) for dopamine, norepinephrine and epinephrine, respectively. The analytical sensitivity, inferred from the slope of the calibration curves established for a range of concentrations between 5 and 125 pg mL(-1), was found to be 0.344, 0.252 and 0.140 dB/pg mL(-1) for dopamine, norepinephrine and epinephrine, respectively. Furthermore, catecholamines speciation with the PS/DVB fiber was completely achieved in 3 min. The analytical performance of the reported sensor was also evaluated and found adequate for catecholamines determination in human urine and plasma samples.
- Published
- 2009
- Full Text
- View/download PDF
22. High performance liquid chromatography coupled to an optical fiber detector coated with laccase for screening catecholamines in plasma and urine.
- Author
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Ferreira FD, Silva LI, Freitas AC, Rocha-Santos TA, and Duarte AC
- Subjects
- Enzymes, Immobilized metabolism, Equipment Design, Female, Humans, Laccase metabolism, Linear Models, Male, Reproducibility of Results, Sensitivity and Specificity, Catecholamines blood, Catecholamines urine, Chromatography, High Pressure Liquid instrumentation, Chromatography, High Pressure Liquid methods, Enzymes, Immobilized chemistry, Laccase chemistry, Optical Fibers
- Abstract
An analytical method based on separation by high performance liquid chromatography (HPLC) and detection by optical fiber (OF) coated with an enzyme (laccase), has been developed for separation and quantification of catecholamines, namely epinephrine, dopamine and norepinephrine. The application of OF as a detector in this analytical system relies on the variation of the reflected optical power detected when the catecholamines eluted from the HPLC column act as the substrate of the laccase immobilized on a tip of a single-mode OF. The developed method shows a high linearity in a range between 5 and 125 pg/mL and detection limits of 3.5, 2.9 and 3.3 pg/mL for epinephrine, dopamine and norepinephrine, respectively. The analytical performance of the proposed method was compared with a classical analytical method, namely high performance liquid chromatography-electrochemical detector (HPLC-ED) regarding catecholamines detection, showing great analytical advantages such as low cost of equipment. Additionally, the proposed method was applied to catecholamines determination in actual samples of plasma and human urine.
- Published
- 2009
- Full Text
- View/download PDF
23. Carbon nanotube field-effect transistor detector associated to gas chromatography for speciation of benzene, toluene, ethylbenzene, (o-, m- and p-)xylene.
- Author
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Silva LI, Ferreira FD, Rocha-Santos TA, and Duarte AC
- Subjects
- Air Pollutants, Occupational analysis, Hydrocarbons, Aromatic analysis, Microchemistry methods, Portugal, Transistors, Electronic, Air Pollutants analysis, Benzene analysis, Benzene Derivatives analysis, Chromatography, Gas instrumentation, Nanotubes, Carbon
- Abstract
An analytical methodology based on a field-effect transistor detector using carbon nanotubes (NTFET) coupled to a gas chromatograph has been developed for the speciation of the following aromatic compounds: benzene, toluene, ethylbenzene, m-xylene, p-xylene and o-xylene (BTEX). This methodology combines the proven separation capability of gas chromatography (GC) with the potential for detection of a NTFET. The developed analyzer shows a high and stable analytical response upon repeated analysis of BTEX during 4 weeks, with detection limit less than 4mug/L. The GC-NTFET system also shows a great suitability for actual monitoring of indoor atmospheres and no significant difference was observed between the results obtained by the developed analyzer and a more classical analytical methodology, namely gas chromatography-flame ionization detection (GC-FID).
- Published
- 2009
- Full Text
- View/download PDF
24. Periablative Anticoagulation Strategies in Patients with Atrial Fibrillation.
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Costa Ferreira FD and Saad EB
- Abstract
Atrial fibrillation is associated with thromboembolic events that may cause important impairment on quality of life. Pulmonary vein isolation is the treatment of choice in cases that are refractory to medical therapy. Once sheaths and catheters are manipulated inside the left atrium, anticoagulation with heparin must be used during the procedure to protect patients from thromboembolic phenomena. Different strategies of anticoagulation are used at different centers. This review summarizes the pathophysiology of thrombus formation in the left atrium, defines which patients are under high risk and describes the main strategies used for anticoagulation.
- Published
- 2008
- Full Text
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25. Molecular detection of HPV 16 and 18 in cervical samples of patients from Belo Horizonte, Minas Gerais, Brazil.
- Author
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Freitas TP, Carmo BB, Paula FD, Rodrigues LF, Fernandes AP, and Fernandes PA
- Subjects
- Brazil, DNA, Viral analysis, Female, Humans, Papillomavirus Infections pathology, Polymerase Chain Reaction, Prospective Studies, Regression Analysis, Severity of Illness Index, Uterine Cervical Neoplasms pathology, Vaginal Smears, Uterine Cervical Dysplasia pathology, Human papillomavirus 16 genetics, Human papillomavirus 18 genetics, Papillomavirus Infections virology, Uterine Cervical Neoplasms virology, Uterine Cervical Dysplasia virology
- Abstract
Purpose: The aim of this study was to investigate the frequency of HPV infection and the types 16 and 18 in cervical samples from patients attended at two public health services of the city of Belo Horizonte, MG., Methods: Cervical samples from 174 patients were collected for cytopathological and molecular tests. HPV infection was searched by PCR utilizing MY09 and MY11 primers or HPV 16 and HPV 18 specific primers., Results: Amongst the 174 samples analyzed, 20.7% presented squamous intraepithelial and/or invasive lesions detected on cytopathological analysis and of those, 94.4% were infected by HPV. HPV 16 was found in 20% of the cases of low-grade squamous intraepithelial lesions and in 40% and 50% of high-grade squamous intraepithelial lesion and squamous invasive carcinoma, respectively. HPV 18 was detected in 6.7% of the low-grade lesion samples and in two HPV16 co-infected samples. In 50% of the cases of high-grade lesion, the HPV type was not determined., Conclusion: The HPV 16 was the virus type more frequently detected. However, more than 50% of the positive samples at the cytopathological analysis were negative for HPV 16 and 18, indicating that possibly other virus types are present in relative high frequencies in the studied population.
- Published
- 2007
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26. Molecular detection of Chlamydia trachomatis and HPV infections in cervical samples with normal and abnormal cytopathological findings.
- Author
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de Paula FD, Fernandes AP, Carmo BB, Vieira DC, Dutra MS, Santos CG, Souza Mdo C, Andrade TC, Vago AR, and Fernandes PA
- Subjects
- Adult, Chlamydia Infections epidemiology, Chlamydia trachomatis isolation & purification, DNA, Viral analysis, Female, Humans, Middle Aged, Papillomaviridae isolation & purification, Papillomavirus Infections epidemiology, Polymerase Chain Reaction, Precancerous Conditions virology, Tumor Virus Infections complications, Uterine Cervical Neoplasms virology, Uterine Cervical Dysplasia virology, Cervix Uteri pathology, Cervix Uteri virology, Chlamydia Infections complications, Papillomavirus Infections complications, Tumor Virus Infections epidemiology
- Abstract
It has been suggested that Chlamydia trachomatis (CT) and human papillomaviruses (HPV) co-infection could contribute to development of intraepithelial lesions. In this study, HPV and CT-DNA were investigated in 250 cervicovaginal samples of patients from Minas Gerais, Brazil. The cytological analysis revealed that 70% of samples (175) were negative, 5.2% (13) presented atypical squamous or glandular cells of undetermined significance (ASCUS/AGUS), 12.4% (31) presented low-grade squamous intraepithelial lesion (LSIL), 10.8% (27) high-grade squamous intraepithelial lesion (HSIL), and 1.6% (4) invasive carcinoma. HPV-DNA and HPV/CT co-infection was observed in 40% (100/250) and in 5.2% (13/250) of samples, respectively. Among the positive cytological samples, HPV-DNA was detected in 73.3% and CT-DNA in 9.33% and in 13%, if only the HPV positive samples were considered. The highest co-infection rate (15.4%) was observed among ASCUS/AGUS samples. Although a significant association was found for HPV infection and the precursor lesions of cervical cancer, it was not possible to establish a significant association between these lesions and CT or HPV/CT co-infection., ((c) 2007 Wiley-Liss, Inc.)
- Published
- 2007
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27. Etiology and incidence facial fractures in children and adults.
- Author
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Montovani JC, de Campos LM, Gomes MA, de Moraes VR, Ferreira FD, and Nogueira EA
- Subjects
- Adolescent, Adult, Aged, Brazil epidemiology, Child, Child, Preschool, Epidemiologic Methods, Female, Humans, Infant, Infant, Newborn, Male, Middle Aged, Facial Bones injuries, Skull Fractures epidemiology, Skull Fractures etiology
- Abstract
Unlabelled: Facial trauma has presented an increasing occurrence in the last four decades, due especially to the growth of accidents with automobiles as well as to the urban violence. Both of which continue being the main cause of such traumas., Aim: To evaluate the features of the population victim of facial trauma as to gender, age, occupation, origin, type of fracture and its cause., Design Study: retrospective clinical with transversal cohort., Material and Method: Retrospective study consulting hospital registers of 513 patients victims of the facial trauma., Results: There was a higher incidence of facial trauma on men (84,9%), white (82,7) and with an average age of 29. Regarding occupation, the trauma was mostly occurred to students (16,6%) and Masons (11,2%). The jaw was the most affected place (35%), followed by zygoma (24%) and by the nose (23%), though most patients presented a single facial fracture (82,5%). Among the causes, accidents with automobiles (28,3%), aggression (21%) and accidental fall s (19,5%) were the most common., Conclusions: Accidents with automobiles continue being the main cause of facial trauma, especially of multiple fractures due to the great transmission of kinetic energy.
- Published
- 2006
- Full Text
- View/download PDF
28. Surgery of frontal sinus fractures: epidemiologic study and evaluation of techniques.
- Author
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Montovani JC, Nogueira EA, Ferreira FD, Lima Neto AC, and Nakajima V
- Subjects
- Adult, Female, Fracture Fixation, Internal, Frontal Sinus surgery, Humans, Male, Middle Aged, Prostheses and Implants, Retrospective Studies, Skull Fractures etiology, Frontal Sinus injuries, Skull Fractures surgery
- Abstract
Unlabelled: The frontal sinus trauma is not rare and it is 8% of the facial fractures. It can affect the anterior and/or posterior plates, with or without hitting the nasofrontal duct. It has a large potential of complications and its management still being a controversy., Objective: To present the casuistic of fractures frontal sinus, the epidemiology and clinical and surgical management of frontal sinus fractures., Materials and Methods: Not randomized retrospective study of 24 patients with frontal sinus fractures Hospital of Clinics, School of Medicine Botucatu, São Paulo, Brazil., Results: From the 24 patients, we had 16 (66,6%) fractures of the extern plate and 8 (33,4%) of both. In 2 patients the nasofrontal duct was involved. Others facial fractures were associated in 20 (83,4%) cases and major lesions of the cerebral segment were found in 13 (54,2%). Subpalpebral incision was performed in the majority with satisfactory aesthetic results. The basis of the surgical treatment was reduction and fixation with different materials (steel wire, mononylon, titanium miniplates) and if necessary we used alogen implants or parietal bone to reconstruct the anterior plate., Conclusion: The principal cause of frontal sinus fractures is crashed car. The management depends of the complexity, because commonly there are cranioencephalic lesions associated. The surgical techniques used are the incisions, bicoronal flap or brow-glabella, infra-orbital rim ("butterfly"), associated a endoscopy sinus surgery in cases of infection, cerebrospinal fluid leak and orbital complications.
- Published
- 2006
- Full Text
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29. Sigmoid cancer adherent to the uterus during pregnancy: case report.
- Author
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Corrêa DS, Lopes A, Ferreira Fd, Nakagawa WT, and Rossi BM
- Published
- 2002
- Full Text
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30. Induction of IgE antibodies with predefined specificity in rhesus monkeys with recombinant birch pollen allergens, Bet v 1 and Bet v 2.
- Author
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Ferreira FD, Mayer P, Sperr WR, Valent P, Seiberler S, Ebner C, Liehl E, Scheiner O, Kraft D, and Valenta R
- Subjects
- Animals, Antibody Formation, Antibody Specificity, Antigens, Plant, Disease Models, Animal, Hypersensitivity immunology, Microfilament Proteins genetics, Plant Proteins genetics, Profilins, Recombinant Proteins genetics, Recombinant Proteins immunology, Allergens immunology, Contractile Proteins, Immunoglobulin E blood, Macaca mulatta immunology, Microfilament Proteins immunology, Plant Proteins immunology, Pollen immunology
- Abstract
Background: Recombinant birch pollen allergens Bet v 1 and Bet v 2 (birch profilin) have been characterized in vitro previously., Objective: To establish a close-to-man model of type I allergy, recombinant birch pollen allergens were injected into rhesus monkeys., Methods: The allergens were expressed in Escherichia coli, purified to homogeneity and injected into rhesus monkeys with aluminium hydroxide as adjuvans. The development of type I allergy was monitored by measurement of specific IgE, in vitro histamine release tests, cellular proliferation assays, skin testing, and bronchial provocation tests., Results: Immunized rhesus monkeys displayed symptoms of type I allergy comparable to those of allergic patients, and cross-reactivity of IgE antibodies with Bet v 1 and Bet v 2 homologous allergens was observed. Systemic application of corticosteroids during secondary immunizations suppressed specific antibody responses., Conclusion: Recombinant birch pollen allergens (Bet v 1 and Bet v 2) were effective to establish a close-to-man model of natural type I allergy in rhesus monkeys, allowing study of specific IgE regulation in vivo.
- Published
- 1996
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31. Purification and characterization of recombinant Bet v I, the major birch pollen allergen. Immunological equivalence to natural Bet v I.
- Author
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Ferreira FD, Hoffmann-Sommergruber K, Breiteneder H, Pettenburger K, Ebner C, Sommergruber W, Steiner R, Bohle B, Sperr WR, and Valent P
- Subjects
- Amino Acid Sequence, Antigens, Plant, Cloning, Molecular, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Humans, Hypersensitivity, Immunoblotting, Immunoglobulin E metabolism, Isoelectric Focusing, Light, Lymphocyte Activation, Molecular Sequence Data, Molecular Weight, Oligodeoxyribonucleotides, Peptide Fragments, Plant Proteins genetics, Plant Proteins immunology, Recombinant Fusion Proteins isolation & purification, Recombinant Proteins immunology, Recombinant Proteins isolation & purification, Scattering, Radiation, Skin Tests, Allergens isolation & purification, Plant Proteins isolation & purification, T-Lymphocytes immunology, Trees immunology
- Abstract
Pollen from trees of the order Fagales (e.g. birch, alder, hazel, oak, and hornbeam) are a major cause of Type I allergies observed in early spring. Previously, we reported the cloning and sequencing of Bet v I, the major birch pollen allergen, which showed high sequence similarities to a family of plant pathogen-activated genes (Breiteneder, H., Pettenburger, K., Bito, A., Valenta, R., Kraft, D., Rumpold, H., Scheiner, O., and Breitenbach, M. (1989) EMBO J. 8, 1935-1938). Here, we present the results on the expression, purification, and characterization of recombinant Bet v I produced in Escherichia coli as fusion and non-fusion protein, respectively. The purified recombinant proteins were analyzed to verify purity and structural integrity, and their immunological properties were compared to those of Bet v I isolated from birch pollen (natural Bet v I). Immunoblot analyses showed that the recombinant proteins are specifically recognized by monoclonal antibodies raised against natural Bet v I as well as by IgE from birch pollen-allergic patients. However, enzyme-linked immunosorbent assays revealed a decreased IgE-binding activity of the recombinant fusion Bet v I compared to the non-fusion and natural Bet v I proteins, which probably results from conformational changes due to the fusion tail. Recombinant non-fusion Bet v I was equivalent to natural Bet v I with respect to IgE-binding properties, the ability to induce in vitro proliferation of allergen-specific T-cell clones, and the ability to release histamine from basophils derived from birch pollen-allergic patients.
- Published
- 1993
32. Differential expression of proline-rich proteins in rabbit salivary glands.
- Author
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Ferreira FD, Robinson R, Hand AR, and Bennick A
- Subjects
- Animals, Blotting, Northern, Blotting, Western, DNA, Electrophoresis, Polyacrylamide Gel, Gene Expression, Immunohistochemistry, Microscopy, Electron, Peptides genetics, Proline-Rich Protein Domains, RNA metabolism, Rabbits, Salivary Glands ultrastructure, Salivary Proteins and Peptides genetics, Peptides metabolism, Salivary Glands metabolism, Salivary Proteins and Peptides metabolism
- Abstract
Salivary glands synthesize and secrete an unusual family of proline-rich proteins (PRPs) that can be broadly divided into acidic and basic PRPs. We studied the tissue-specific expression of these proteins in rabbits, using antibodies to rabbit acidic and basic PRPs as well as antibodies and cDNA probes to human PRPs. By immunoblotting, in vitro translation, and Northern blotting, basic PRPs could be readily detected in the parotid gland but were absent in other salivary glands. In contrast, synthesis in vitro of acidic PRPs was detected in parotid, sublingual, and submandibular glands. Ultrastructural localization with immunogold showed heavy labeling with antibodies to acidic PRPs of secretory granules of parotid acinar cells and sublingual serous demilune cells. Less intense labeling occurred in the seromucous acinar cells of the submandibular gland. With antibodies to basic PRPs, the labeling of the parotid gland was similar to that observed with antibodies to acidic PRPs, but there was only weak labeling of granules of a few sublingual demilune cells, and no labeling of the submandibular gland. These results demonstrate a variable pattern of distribution of acidic and basic PRPs in rabbit salivary glands. These animals are therefore well suited for study of differential tissue expression of PRPs.
- Published
- 1992
- Full Text
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33. Adenine nucleotide changes in submandibular salivary glands of rats following isoproterenol or incisor tooth amputation.
- Author
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Nicolau J and Ferreira FD
- Subjects
- Adenosine Diphosphate metabolism, Adenosine Monophosphate metabolism, Adenosine Triphosphate metabolism, Animals, Dose-Response Relationship, Drug, Male, Rats, Submandibular Gland drug effects, Adenine Nucleotides metabolism, Incisor surgery, Isoproterenol pharmacology, Submandibular Gland metabolism
- Abstract
Male rats (180-220 g) were injected daily with isoproterenol (2 mg/kg of body weight) for up to 6 days, or their incisor teeth were amputated on every other day for up to six amputations. The animals were subdivided into groups killed 12 or 24 h after the first or last intervention. In the development of sialadenosis caused by isoproterenol, the levels of ATP were greater (13-30%), while those of AMP were lower (13-19%) in the experimental groups. No variation was noted in ADP content. In tooth-amputated animals, only the five and six amputation subgroups showed higher values for ATP (approx. 17%), and ADP (12 and 15%, respectively). The inorganic phosphate level was lower in both experimental groups (between 11-28% for isoproterenol and 13-22% for amputation). Thus isoproterenol caused different metabolic responses in submandibular salivary glands from those induced by incisor amputation.
- Published
- 1989
- Full Text
- View/download PDF
34. Different responses of the submandibular salivary glands and liver of rats subjected to excess vitamin A: study on glycogen metabolism.
- Author
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Ferreira FD and Nicolau J
- Subjects
- Animals, Body Weight, Liver drug effects, Liver Glycogen metabolism, Male, Phosphorylase a metabolism, Phosphorylases metabolism, Rats, Rats, Inbred Strains, Submandibular Gland drug effects, Vitamin A metabolism, Glycogen metabolism, Hypervitaminosis A, Liver metabolism, Submandibular Gland metabolism
- Abstract
The effect of excess vitamin A on glycogen metabolism in the submandibular salivary gland, as compared to that in liver was studied. Contrary to what has been previously reported for the liver, either using from 1 to 6 X 15 000 or 2 X 30 000 U.I. vitamin A, the glycogen content in the submandibular salivary gland was lower in the hypervitaminotic rats. The total phosphorylase activity was reduced in the groups receiving lower vitamin A doses.
- Published
- 1985
35. Adenine nucleotide contents and ATPases activities in porcine deciduous dental pulp during the root formation, in fully formed root and during root resorption phases.
- Author
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Paiva-Novaes MS, Ferreira FD, and Nicolau J
- Subjects
- Adenosine Diphosphate analysis, Adenosine Monophosphate analysis, Adenosine Triphosphate analysis, Animals, Dental Pulp enzymology, Female, Male, Molar enzymology, Root Resorption enzymology, Swine, Tooth Root embryology, Tooth Root enzymology, Tooth, Deciduous enzymology, Adenine Nucleotides analysis, Aging physiology, Ca(2+) Mg(2+)-ATPase analysis, Calcium-Transporting ATPases analysis, Dental Pulp analysis, Molar analysis, Root Resorption physiopathology, Tooth Root analysis, Tooth, Deciduous analysis
- Abstract
Nucleotide content and activity of certain enzymes were compared in pigs of various ages in order to study the energetic metabolism of deciduous dental pulps in the three phases of the cycle of tooth ontogeny, namely, root formation, fully formed root and root resorption phases. The frozen pulps were removed with the help of a screw vise and analysed for ATP, ADP and AMP contents and Ca2+ and Mg2+-ATPases activities. The highest ATP content in the first deciduous molar pulp was found when the tooth was still in an intrabony position. The calculated energy charge, although low for all groups, at this stage of development, indicated an activation of the consuming processes. In the root resorption phase, lowest ATP content and higher Ca2+ and Mg2+-ATPases activities were observed.
- Published
- 1989
36. Changes in glucose metabolism in submandibular salivary glands of rats after isoproterenol or incisor-tooth amputation.
- Author
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Ferreira FD and Nicolau J
- Subjects
- Amputation, Surgical, Animals, Hexokinase metabolism, Incisor surgery, Isoproterenol pharmacokinetics, Male, Pyruvate Kinase metabolism, Rats, Rats, Inbred Strains, Submandibular Gland drug effects, Glucose metabolism, Salivary Gland Diseases metabolism, Submandibular Gland metabolism
- Abstract
Rats were injected daily with isoproterenol (2 mg/kg of body wt) for up to five days, or their incisor teeth were amputated on every other day for up to five amputations. The animals were subdivided in two subgroups killed 12 or 24 h after the first or last intervention. At 12 h all enzymes except hexokinase (HK) and pyruvate kinase (PFK) showed decreased activities after isoproterenol. After incisor amputation, only increased HK and PFK activities were observed. With both procedures, there is activation of beta-adrenergic receptors but results show that different biochemical events take place, suggesting different mechanisms.
- Published
- 1987
- Full Text
- View/download PDF
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