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High performance liquid chromatography coupled to an optical fiber detector coated with laccase for screening catecholamines in plasma and urine.
- Source :
-
Journal of chromatography. A [J Chromatogr A] 2009 Oct 16; Vol. 1216 (42), pp. 7049-54. Date of Electronic Publication: 2009 Aug 31. - Publication Year :
- 2009
-
Abstract
- An analytical method based on separation by high performance liquid chromatography (HPLC) and detection by optical fiber (OF) coated with an enzyme (laccase), has been developed for separation and quantification of catecholamines, namely epinephrine, dopamine and norepinephrine. The application of OF as a detector in this analytical system relies on the variation of the reflected optical power detected when the catecholamines eluted from the HPLC column act as the substrate of the laccase immobilized on a tip of a single-mode OF. The developed method shows a high linearity in a range between 5 and 125 pg/mL and detection limits of 3.5, 2.9 and 3.3 pg/mL for epinephrine, dopamine and norepinephrine, respectively. The analytical performance of the proposed method was compared with a classical analytical method, namely high performance liquid chromatography-electrochemical detector (HPLC-ED) regarding catecholamines detection, showing great analytical advantages such as low cost of equipment. Additionally, the proposed method was applied to catecholamines determination in actual samples of plasma and human urine.
- Subjects :
- Enzymes, Immobilized metabolism
Equipment Design
Female
Humans
Laccase metabolism
Linear Models
Male
Reproducibility of Results
Sensitivity and Specificity
Catecholamines blood
Catecholamines urine
Chromatography, High Pressure Liquid instrumentation
Chromatography, High Pressure Liquid methods
Enzymes, Immobilized chemistry
Laccase chemistry
Optical Fibers
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3778
- Volume :
- 1216
- Issue :
- 42
- Database :
- MEDLINE
- Journal :
- Journal of chromatography. A
- Publication Type :
- Academic Journal
- Accession number :
- 19747685
- Full Text :
- https://doi.org/10.1016/j.chroma.2009.08.067