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5 results on '"Feng-Biao Wang"'

1. Production, Biosynthesis, and Commercial Applications of Fatty Acids From Oleaginous Fungi

Author

Xin-Yue Zhang, Bing Li, Bei-Chen Huang, Feng-Biao Wang, Yue-Qi Zhang, Shao-Geng Zhao, Min Li, Hai-Ying Wang, Xin-Jun Yu, Xiao-Yan Liu, Jing Jiang, and Zhi-Peng Wang

Subjects
oleaginous fungi, triacylglycerols, regulation strategy, fatty acids, Commercial application, Nutrition. Foods and food supply, TX341-641
Abstract

Oleaginous fungi (including fungus-like protists) are attractive in lipid production due to their short growth cycle, large biomass and high yield of lipids. Some typical oleaginous fungi including Galactomyces geotrichum, Thraustochytrids, Mortierella isabellina, and Mucor circinelloides, have been well studied for the ability to accumulate fatty acids with commercial application. Here, we review recent progress toward fermentation, extraction, of fungal fatty acids. To reduce cost of the fatty acids, fatty acid productions from raw materials were also summarized. Then, the synthesis mechanism of fatty acids was introduced. We also review recent studies of the metabolic engineering strategies have been developed as efficient tools in oleaginous fungi to overcome the biochemical limit and to improve production efficiency of the special fatty acids. It also can be predictable that metabolic engineering can further enhance biosynthesis of fatty acids and change the storage mode of fatty acids.

Published
2022
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2. Characterization of a New Intracellular Alginate Lyase with Metal Ions-Tolerant and pH-Stable Properties

Author

Yan Ma, Jie Li, Xin-Yue Zhang, Hao-Dong Ni, Feng-Biao Wang, Hai-Ying Wang, and Zhi-Peng Wang

Subjects
Alginate lyase, Intracellular, pH-stable, Metal ion-resisted, Yarrowia lipolytica, Biology (General), QH301-705.5
Abstract

Alginate lyases play an important role in alginate oligosaccharides (AOS) preparation and brown seaweed processing. Many extracellular alginate lyases have been characterized to develop efficient degradation tools needed for industrial applications. However, few studies focusing on intracellular alginate lyases have been conducted. In this work, a novel intracellular alkaline alginate lyase Alyw202 from Vibrio sp. W2 was cloned, expressed and characterized. Secretory expression was performed in a food-grade host, Yarrowia lipolytica. Recombinant Alyw202 with a molecular weight of approximately 38.3 kDa exhibited the highest activity at 45 °C and more than 60% of the activity in a broad pH range of 3.0 to 10.0. Furthermore, Alyw202 showed remarkable metal ion-tolerance, NaCl independence and the capacity of degrading alginate into oligosaccharides of DP2-DP4. Due to the unique pH-stable and high salt-tolerant properties, Alyw202 has potential applications in the food and pharmaceutical industries.

Published
2020
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3. Characterization of a New Intracellular Alginate Lyase with Metal Ions-Tolerant and pH-Stable Properties

Author

Hao-Dong Ni, Xin-Yue Zhang, Jie Li, Feng-Biao Wang, Yan Ma, Hai-Ying Wang, and Zhi-Peng Wang

Subjects
Yarrowia lipolytica, Alginates, Metal ions in aqueous solution, Pharmaceutical Science, Sodium Chloride, 01 natural sciences, Article, Catalysis, Substrate Specificity, Alginate lyase, law.invention, Metal ion-resisted, Metal, 03 medical and health sciences, Bacterial Proteins, law, Enzyme Stability, Drug Discovery, Extracellular, pH-stable, lcsh:QH301-705.5, Pharmacology, Toxicology and Pharmaceutics (miscellaneous), Polysaccharide-Lyases, Vibrio, 030304 developmental biology, Ions, 0303 health sciences, biology, 010405 organic chemistry, Chemistry, Temperature, Yarrowia, Hydrogen-Ion Concentration, biology.organism_classification, Recombinant Proteins, Intracellular, 0104 chemical sciences, lcsh:Biology (General), Biochemistry, visual_art, visual_art.visual_art_medium, Recombinant DNA, Degradation (geology)
Abstract

Alginate lyases play an important role in alginate oligosaccharides (AOS) preparation and brown seaweed processing. Many extracellular alginate lyases have been characterized to develop efficient degradation tools needed for industrial applications. However, few studies focusing on intracellular alginate lyases have been conducted. In this work, a novel intracellular alkaline alginate lyase Alyw202 from Vibrio sp. W2 was cloned, expressed and characterized. Secretory expression was performed in a food-grade host, Yarrowia lipolytica. Recombinant Alyw202 with a molecular weight of approximately 38.3 kDa exhibited the highest activity at 45 &deg, C and more than 60% of the activity in a broad pH range of 3.0 to 10.0. Furthermore, Alyw202 showed remarkable metal ion-tolerance, NaCl independence and the capacity of degrading alginate into oligosaccharides of DP2-DP4. Due to the unique pH-stable and high salt-tolerant properties, Alyw202 has potential applications in the food and pharmaceutical industries.

Published
2020

4. Fracture Mechanics Study on Porosity Zirnocia/hydroxyapatite Coating Obtained through Microarc Oxidation on Medical Titanium Alloy Surface

Author

Jie Yu, Shi Chun Di, and Feng Biao Wang

Subjects
Materials science, General Engineering, Titanium alloy, Fracture mechanics, Electrolyte, engineering.material, Coating, visual_art, Phase (matter), engineering, visual_art.visual_art_medium, Cubic zirconia, Ceramic, Composite material, Porosity
Abstract

In electrolytes TiO2-based complex ceramic coatings containing Ca and P on titanium alloy were ultimately formed containing zirconia/hydroxyapatite by Micro-arc oxidation process. As well as the biomechanics measure experiment of speciments had been done, and the datas were analysised and researched. In addition, the toughening mechanism of reinforced coating was studied in detail, and consists of the second phase toughening, phase transformation toughening and microcrack toughening.The results show that the zirconia /hydroxyapatite coating has advanced biomechanics propertys compared to the individual hydroxyapatite one.

Published
2012

5. Biocompatibility Research of Medicine Titanium Alloy Coating by Microarc Oxidation

Author

Shi Chun Di, Feng Biao Wang, and Jie Yu

Subjects
Materials science, Biocompatibility, Scanning electron microscope, Simulated body fluid, Metallurgy, technology, industry, and agriculture, General Engineering, Titanium alloy, engineering.material, Ceramic coating, Coating, Chemical engineering, engineering, Hydroxyapatite coating, Oxidation process
Abstract

A medicinal titanium alloy hydroxyapatite coating was fabricated by microarc oxidation process, and a kind standard of simulated body fluid was configured, after that the biocompatibility experiment was carried out. The soaked coatings surface morphologies were observed and analysed by scanning electron microscopy,and the coating spectra were simultaneityly researched by X-ray diffraction. The instance of degradation and precipitate were investigated,and the transformation of Ca/P value were measured.

Published
2011

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