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In search of innovative antifungal solutions for the control of plant diseases, a series of cyanoacrylate derivatives containing naphthalene groups were designed and synthesized, and their inhibition activity against four plant pathogenic fungi was evaluated. The results of in vitro bioassay revealed that some target compounds possessed obvious antifungal effect against Fusarium graminearum. As the most prominent one, compound A2 showed a inhibition rate of 98.46 % at 10 µg/mL and an EC50 value of 0.26 µg/mL, which was close to that of the positive control phenamacril (with a inhibition rate of 100 % at 10 µg/mL and EC50 value of 0.14 µg/mL). The compound A2 also markedly inhibited the growth of F. graminearum inoculated on rice leaves at 200 μg/mL with the protective and curative efficiencies of 89.03 % and 90.91 %, respectively, which were close to that of the positive control phenamacril (with the protective and curative efficiencies of 94.54 % and 96.36 %, respectively). The observation under scanning electron microscopy and measurement of relative conductivity revealed that compound A2 caused the hyphal surface become shrunken and rough, and made the cell membrane permeability increased. Molecular docking and molecular dynamics simulation analyses showed that compound A2 interacted with the key residues in the active site of myosin-5 in a similar mode as phenamacril. These results suggested that target compounds were potential myosin-5 inhibitors, they could serve as the lead compounds for further structural optimization to develop new fungicides against F. graminearum.

Abstract Polymer dielectrics that perform efficiently under harsh electrification conditions are critical elements of advanced electronic and power systems. However, developing polymer dielectrics capable of reliably withstanding harsh temperatures and electric fields remains a fundamental challenge, requiring a delicate balance in dielectric constant (K), breakdown strength (Eb), and thermal parameters. Here, amide crosslinking networks into cyano polymers is introduced, forming asymmetric dipole pairs with differing dipole moments. This strategy weakens the original electrostatic interactions between dipoles, thereby reducing the dipole orientation barriers of cyano groups, achieving dipole activation while suppressing polarization losses. The resulting styrene‐acrylonitrile/crosslinking styrene‐maleic anhydride (SAN/CSMA) blends exhibit a K of 4.35 and an Eb of 670 MV m−1 simultaneously at 120 °C, and ultrahigh discharged energy densities (Ue) with 90% efficiency of 8.6 and 7.4 J cm−3 at 120 and 150 °C are achieved, respectively, more than ten times that of the original dielectric at the same conditions. The SAN/CSMA blends show excellent cyclic stability in harsh conditions. Combining the results with SAN/CSMA and ABS (acrylonitrile‐butadiene‐styrene copolymer)/CSMA blends, it is demonstrated that this novel strategy can meet the demands of high‐performing dielectric polymers at elevated temperatures.

Monolayer 2D metal‐organic framework (MOF) nanosheets, characterized by abundant exposed active sites and tunable structure and function (such as altering the metal nodes or organic ligands), have emerged as a pivotal class of 2D materials, demonstrating irreplaceable applications across diverse research domains in materials and chemistry. This review provides a comprehensive survey of the latest research progress in the synthesis of monolayer 2D MOF nanosheets. Specifically, recent synthetic strategies, including top‐down and bottom‐up methods, are delved and their applications in gas separation, catalysis, sensing platforms, and energy storage are explored. Additionally, the challenges faced in the investigation of monolayer 2D MOF nanosheets are elucidated and future opportunities for these materials as a novel generation of 2D materials are outlined.

Abstract Bioelectronic medicine is a rapidly growing field where targeted electrical signals can act as an adjunct or alternative to drugs to treat neurological disorders and diseases via stimulating the peripheral nervous system on demand. However, current existing strategies are limited by external battery requirements, and the injury and inflammation caused by the mechanical mismatch between rigid electrodes and soft nerves. Here we report a wireless, leadless, and battery-free ferroelectret implant, termed NeuroRing, that wraps around the target peripheral nerve and demonstrates high mechanical conformability to dynamic motion nerve tissue. As-fabricated NeuroRing can act as an ultrasound receiver that converts ultrasound vibrations into electrostimulation pulses, thus stimulating the targeted peripheral nerve on demand. This capability is demonstrated by the precise modulation of the sacral splanchnic nerve to treat colitis, providing a framework for future bioelectronic medicines that offer an alternative to non-specific pharmacological approaches.

P2X receptors are extracellular ATP-gated ion channels that form homo- or heterotrimers and consist of seven subtypes. They are expressed in various tissues, including neuronal and nonneuronal cells, and play critical roles in physiological processes such as neurotransmission, inflammation, pain, and cancer. As a result, P2X receptors have attracted considerable interest as drug targets, and various competitive inhibitors have been developed. However, although several P2X receptor structures from different subtypes have been reported, the limited structural information of P2X receptors in complex with competitive antagonists hampers the understanding of orthosteric inhibition, hindering the further design and optimization of those antagonists for drug discovery. We determined the cryogenic electron microscopy (cryo-EM) structures of the mammalian P2X7 receptor in complex with two classical competitive antagonists of pyridoxal-5'-phosphate derivatives, pyridoxal-5'-phosphate-6-(2'-naphthylazo-6'-nitro-4',8'-disulfonate) (PPNDS) and pyridoxal phosphate-6-azophenyl-2′,5′-disulfonic acid (PPADS), and performed structure-based mutational analysis by patch-clamp recording as well as molecular dynamics (MD) simulations. Our structures revealed the orthosteric site for PPADS/PPNDS, and structural comparison with the previously reported apo- and ATP-bound structures showed how PPADS/PPNDS binding inhibits the conformational changes associated with channel activation. In addition, structure-based mutational analysis identified key residues involved in the PPNDS sensitivity of P2X1 and P2X3, which are known to have higher affinity for PPADS/PPNDS than other P2X subtypes.

Abstract Background Sepsis is a systemic inflammatory response syndrome caused by severe infection in children, but cases of sepsis associated with human parainfluenza virus (HPIV) have been rarely reported in newborns. Case presentation We report a case of HPIV-3 positive full-term newborn admitted to the Neonatal Intensive Care Unit of Beijing Children’s Hospital due to hematuria, gloomy spirit, inactivity and loss of appetite for 6 h. He had septic shock when he arrived the Accident & Emergency Department requiring immediate intubation and mechanical ventilation. Intravenous antibiotics were started. He had completely negative response to all anti-shock treatments including fluid resuscitation and vasopressor supports, and died 14 h later. Viral nucleic acid detection and metagenomic next-generation sequencing (mNGS) analyses of nasopharyngeal aspirate and blood specimens verified an HPIV-3 infection, with negative bacterial culture results. The HPIV-3 strain detected in this patient was subtyped as HPIV C3a, and two unreported amino acid mutations were found in the HN protein region. Conclusion The patient had a severe infection associated with HPIV-3, which was the cause of sepsis and septic shock. This study showed the diagnostic value of mNGS in etiological diagnosis, especially in severe neonatal case.

The stability of the backfill barricade is an important foundation for safe mining production. The backfill material is transported to the underground mined-out area. During the backfilling process, it is difficult to directly monitor the curing process of backfill body and its lateral load on barricades. This severely affects the accurate formulation of the mining and backfilling strategy as well as effective control of associated costs. In order to investigate the change law of pressure on barricade, a continuous monitoring experiment was conducted by installing pressure sensors inside the barricade. The results indicated that the pressure on barricade showed an initial increase followed by a subsequent decrease and eventual convergence towards zero with the increase in backfill height and curing time. A barricade pressure prediction model was established for discontinuous backfilling conditions using a fitting method that characterizes the relationship between backfill height and barricade pressure through a Gaussian function. Finally, from both on-site monitoring and theoretical analysis, it was jointly demonstrated that the continuous filling of Luohe iron ore mine quarry has safety and feasibility.

Although many dielectric polymers exhibit high energy storage density (Ue) with enhanced dipolar polarization at room temperature, the substantially increased electric conduction loss at high applied electric fields and high temperatures remains a great challenge. Here, we report a strategy that high contents of medium-polar ester group and end-group (St) modification are introduced into a biodegradable polymer polylactic acid (PLA) to synergistically reduce the loss and enhance Ue and charge-discharge efficiency (η). The resultant St-modified PLA polymer (PLA-St) exhibits an Ue of 6.5 J/cm3 with an ultra-high η (95.4%), far outperforming the best reported dielectric polymers. It is worth noting that the modified molecular structures can generate deep trap centers and restrict the local dipole motions in the polymer, which are responsible for the reduction of conduction loss and improvements in high-temperature capacitive performance. In addition, the PLA-St polymer shows intrinsically excellent self-healing ability and cyclic stability surviving over 500 000 charge-discharge cycles. This work offers an efficient route to next-generation eco-friendly dielectric polymers with high energy density, low loss, and long-term stability.

Coral sand is widely encountered in coastal areas of tropical and subtropical regions. Compared with silica sand, it usually exhibits weaker performance from the perspective of engineering geology. To improve the geomechanical performance of coral sand and meet the requirement of foundation construction in coastal areas, a novel alkali activation-based sustainable binder was developed. The alkali-activated slag (AAS) binder material was composed of ground granulated blast-furnace slag (GGBS) and hydrated lime with the amendment of biochar, an agricultural waste-derived material. The biochar-amended AAS stabilized coral sand was subjected to a series of laboratory tests to determine its mechanical, physicochemical, and microstructural characteristics. Results show that adding a moderate amount of biochar in AAS could improve soil strength, elastic modulus, and water holding capacity by up to 20%, 70%, and 30%, respectively. Moreover, the addition of biochar in AAS had a marginal effect on the sulfate resistance of the stabilized sand, especially at high biochar content. However, the resistance of the AAS stabilized sand to wet-dry cycles slightly deteriorated with the addition of biochar. Based on these observations, a conceptual model showing biochar-AAS-sand interactions was proposed, in which biochar served as an internal curing agent, micro-reinforcer, and mechanically weak point.

Nsp13, a non-structural protein belonging to the coronavirus family 1B (SF1B) helicase, exhibits 5′–3′ polarity-dependent DNA or RNA unwinding using NTPs. Crucially, it serves as a key component of the viral replication–transcription complex (RTC), playing an indispensable role in the coronavirus life cycle and thereby making it a promising target for broad-spectrum antiviral therapies. The imidazole scaffold, known for its antiviral potential, has been proposed as a potential scaffold. In this study, a fluorescence-based assay was designed by labeling dsDNA substrates with a commercial fluorophore and monitoring signal changes upon Nsp13 helicase activity. Optimization and high-throughput screening validated the feasibility of this approach. In accordance with the structural characteristics of ADP, we employed a structural-based design strategy to synthesize three classes of imidazole-based compounds through substitution reaction. Through in vitro activity research, pharmacokinetic parameter analysis, and molecular docking simulation, we identified compounds A16 (IC50 = 1.25 μM) and B3 (IC50 = 0.98 μM) as potential lead antiviral compounds for further targeted drug research.

The improvement of the AGC regulation capability of thermal power plants is very important for the secure and stable operation of the power grid, especially in the situation of large-scale renewable energy access to the power grid. In this study, the prediction and optimization for the AGC regulation capability of thermal power plants is proposed. Firstly, considering parameters related to the AGC regulation of the thermal power plant, the max-relevance and min-redundancy (mRMR) is used to extract features from historical sequences of the parameters. Next, a model with multi-long short-term neural networks (mLSTM) is constructed to predict the AGC regulation capability; that is, the obtained feature set is considered as the inputs of the first LSTM sub-model to predict future values of the main steam pressure and main steam temperature, which are then utilized as the inputs of the second LSTM sub-model to predict the actual power generation during AGC regulation operation. Then, the AGC regulation index is calculated according to the “management rules of grid-connected operation of power plant in Northern China” and “management rules of auxiliary service of the grid-connected power plant in Northern China” (i.e., “two rules”), and it is then considered as the objective function to be maximized by optimizing the coal feed rate, air supply rate, and feedwater flow rate. Finally, the actual AGC regulation process of a 300 MW coal-fired power plant is used as an application, and the results show that the proposed method can effectively predict and improve the regulation capability when the AGC instruction is received from the power grid.

Gut microbiota plays a critical role in regulating the health and adaptation of wildlife. However, our understanding of how exposure to environmental heavy metals influences the gut microbiota of wild birds, particularly during the vulnerable and sensitive nestling stage, remains limited. In order to investigate the relationship between heavy metals and the gut microbiota, we analyzed the characteristics of gut microbiota and heavy metals levels in tree sparrow nestlings at different ages (6, 9 and 12-day-old). The study was conducted in two distinct areas: Baiyin (BY), which is heavily contaminated with heavy metals, and Liujiaxia (LJX), a relatively unpolluted area. Our result reveled a decrease in gut microbiota diversity and increased inter-individual variation among nestlings in BY. However, we also observed an increase in the abundance of bacterial groups and an up-regulation of bacterial metabolic functions associated with resistance to heavy metals toxicity in BY. Furthermore, we identified a metal-associated shift in the relative abundance of microbial taxa in 12-day-old tree sparrow nestlings in BY, particularly involving Aeromonadaceae, Ruminococcaceae and Pseudomonadaceae. Moreover, a significant positive correlation was found between the body condition of tree sparrow nestlings and the abundance of Bifidobacteriaceae in BY. Collectively, our findings indicate that the gut microbiota of tree sparrow nestlings is susceptible to heavy metals during early development. However, the results also highlight the presence of adaptive responses that enable them to effectively cope with environmental heavy metal pollution.

In high-resolution remote sensing images, road scale diversity and occlusions caused by shadows, buildings, and vegetation often pose challenges for road extraction. Currently, end-to-end models constructed using deep convolutional neural networks are widely used in road extraction and have significantly improved the accuracy of this task. However, the connectivity and completeness of their results require improvement. This article proposes a dual task-driven deep convolutional neural network constructed by combining road shape patterns and scale differences. The mainline task is road-surface segmentation, the encoder of which employs residual convolution for feature extraction. The decoder comprises a multiscale and multidirection strip convolution module, the output of which is the final extraction result. The splitting task is road centerline extraction, the input features of which come from the coding layer of the road-surface segmentation branches. The intermediate features are incorporated into the decoder of the road-surface segmentation branches, to fully exploit the road centerline and thus improve the road-surface segmentation result connectivity. Implementation of the proposed method on the CHN6-CUG and DeepGlobe datasets reveals superior performance to comparative methods as regards quantitative evaluation metrics; evident advantages for road coverings, road intersections, and low-scale roads; greater model portability; and better small-sample learning capability.

In order to solve the problem of insufficient heating heat in solar thermal system and soil source heat pump system, and the problem of high energy consumption caused by traditional control mode in combined heating system, a multi-energy complementary solar thermal supplementary heating system controlled by fuzzy controller was proposed. The operating mode of the whole heating system was analyzed, and the relevant fuzzy model was established. TRNSYS and MATLAB simulation platform were used to simulate the system. The results show that the average performance coefficient of COPs(Coefficient of performance) is improved by 0.27 and the total energy consumption is reduced by 199.88kWh in 5 days compared with the traditional control system, which has the advantages of improving system performance and saving energy. At the same time, it is verified that the photothermal system can effectively maintain the long-term balance of soil temperature and make the heating system run efficiently for a long time.

Designing wereable neural invasive electrical stimulation system remains a challenge. Here, researchers provide an effective technology platform for the elimination of tricky neural stimulus-inertia using bionic electronic modulation, which is a significant step forward for long-lasting treatment of nervous system diseases.

Rotavirus remains a major cause of diarrhea among 5-y-old children, and vaccination is currently the most effective and economical measure. We conducted a randomized, double-blind, placebo-controlled phase II clinical trial designed to determine the dosage, immunogenicity, and safety profile of a novel hexavalent rotavirus vaccine. In total, 480 eligible healthy infants, who were 6–12 weeks of age at the time of randomization were randomly allocated (1:1:1) to receive 105.5 focus-forming unit (FFU) or 106.5FFU of vaccine or placebo on a 0, 28 and 56-d schedule. Blood samples were collected 28 d after the third dose to assess rotavirus immunoglobulin A (IgA) antibody levels. Adverse events (AEs) up to 28 d after each dose and serious adverse events (SAEs) up to 6 months after the third dose were recorded as safety measurements. The anti-rotavirus IgA seroconversion rate of the vaccine groups reached more than 70.00%, ranging from 74.63% to 76.87%. The postdose 3 (PD3) geometric mean concentrations (GMCs) of anti-rotavirus IgA among vaccine recipients ranged from 76.97 U/ml to 84.46 U/ml. At least one solicited AE was recorded in 114 infants (71.25%) in the high-dose vaccine group, 106 infants (66.25%) in the low-dose vaccine group and 104 infants (65.00%) in the placebo group. The most frequently solicited AE was fever. The novel oral hexavalent rotavirus vaccine was safe and immunogenic in infants support the conclusion to advance the candidate vaccine for phase 3 efficacy trials.

ObjectiveMalassezia furfur (M. furfur) is a lipophilic, conditionally pathogenic yeast that mainly causes skin infections, but the reports of related invasive infections are increasing. The aim of this study is to provide clinical data to assist physicians in the management of patients with invasive infections caused by M. furfur.MethodsA case of pulmonary infection caused by M. furfur in a hematopoietic stem cell transplant patient for aplastic anemia was reported. In addition, the literature on invasive infection by M. furfur published in PubMed and Web of Science in English until 31 July 2022 was reviewed.ResultsClinical data analysis of 86 patients (from 37 studies and our case) revealed that most of them were preterm (44.2%), followed by adults (31.4%). M. furfur fungemia occurred in 79.1% of the 86 patients, and 45 of them were clearly obtained from catheter blood. Other patients developed catheter-related infections, pneumonia, peripheral thromboembolism, endocarditis, meningitis, peritonitis and disseminated infections. Thirty-eight preterm infants had underlying diseases such as very low birth weight and/or multiple organ hypoplasia. The remaining patients had compromised immunity or severe gastrointestinal diseases. 97.7% of patients underwent invasive procedures and 80.2% received total parenteral nutrition (TPN). Fever, thrombocytopenia and leukocytosis accounted for 55.8%, 38.4% and 24.4% of patients with M. furfur invasive infections, respectively. 69.8% of the patients received antifungal therapy, mainly amphotericin B (AmB) or azoles. Of 84 patients with indwelling catheters, 58.3% underwent the removal of catheters. TPN were discontinued in 30 of 69 patients. The all-cause mortality of 86 patients was 27.9%.ConclusionsM. furfur can cause a variety of invasive infections. These patients mostly occur in premature infants, low immunity and severe gastrointestinal diseases. Indwelling catheters and TPN infusion are major risk factors. AmB, l-AmB and azoles are the most commonly used agents, and simultaneous removal of the catheter and termination of TPN infusion are important for the treatment of M. furfur invasive infections.

In this work, oxygen vacancy-regulated La0.7Ca0.3MnO3-δ:Ag (LCMO:A) nanocomposite thin films on LaAlO3 (001) substrates were investigated to obtain films with large temperature coefficient of resistance (TCR) values. LCMO:A nanocomposite thin films were synthesized using pulsed laser deposition, and oxygen pressures during film deposition and annealing steps were optimized. As oxygen pressures increased, lattice parameter increased from 70 Pa to 100 Pa, Tp increased monotonically from 168 K to 282 K, and average Mn4+ concentration in the film increased as indicated by X-ray photoemission spectroscopy data. Record high TCR value of ∼37% K−1 was achieved in LCMO:A nanocomposite thin film prepared with optimal oxygen pressures, making this film promising candidate for applications in bolometers.

Methanol steam reforming manifests great potential for generating hydrogen owing to its lower reaction temperature (200–300 °C) and higher hydrogen/carbon ratio comparing with ethanol and methane reforming. In this case, methanol steam reforming is applied in various renewable energy systems to assist the energy conversion and improve the system efficiency. The performance of methanol steam reforming reaction strongly depends on the catalysts and reactor structure. In this paper, the development of the copper-based, the noble metal-based and the nanomaterial catalysts were summarized by analyzing the effects of different modification methods, which indicates that cutting the cost and simplifying the manufacturing process are the future goal of catalyst modification. Moreover, the reaction mechanism of different catalyst types was discussed. For the reactor performance, conventional, miniature, micro, and membrane reactors were discussed and compared, where conventional reactor with high CO tolerance is more suitable for industrial application while membrane reactor with high H2 purity and compact structure is ideal for fuel cell technology. The integration of the methanol steam reforming system into renewable power systems was reviewed as well. Methanol steam reforming technology is of great potential in exhaust heat recovery, cogeneration system and other renewable energy field, where more comprehensive researches should be performed.

Measuring the nitrogen oxides concentration accurately at the inlet of the selective catalytic reduction denitrification system plays an important role in controlling the nitrogen oxides concentration for coal-fired power plants, and a coupling relationship exists between nitrogen oxides concentration and multiple operational variables. Here, a modeling method based on feature fusion and long short-term memory network is proposed to mine the spatial and temporal coupling relationship between input variables for improving the prediction accuracy. First, the collected data were converted to image-like sequences. Then, the high-dimensional features of image-like sequences were fused by a convolutional neural network, and the spatial coupling features among the variables were mined. Finally, the constructed fusion features were input into the long short-term memory network to further explore the time coupling characteristics among the variables and complete the prediction of nitrogen oxides concentration at the inlet of the selective catalytic reduction denitrification system. The simulation results show that the prediction error of nitrogen oxides concentration at the inlet of selective catalytic reduction denitrification system based on CNN-LSTM model is 15.15% lower than that of traditional LSTM model.

Developing highly efficient flexible microwave absorber is of great significance for wearable electronics and aerospace applications. In this work, the fusiform SnO2 nanocrystals film in situ grown on flexible carbon fiber cloth is rationally designed and fabricated through combining air calcination and hydrothermal synthesis. X-ray photoelectron spectrum confirms fusiform SnO2 nanocrystals film and carbon fiber cloth are effectively integrated with strong chemical bonds of COSn. The as-prepared composite exhibits strong reflection loss of −49.1 dB (2.6 GHz) and wide effective absorption bandwidth of 5.8 GHz (11.6–17.4 GHz) with a thin matched thickness of 1.6 mm, surpassing to pure carbon fiber cloth and many SnO2/carbon-based microwave absorbers. The efficient performance originates from well-matched characteristic impedance and multifarious electromagnetic attenuation mechanisms, i.g., dipole orientation polarization, interfacial polarization relaxation, conductive loss, and multiple reflections/scatterings. Especially, differential charge density calculation reveals the uneven charge distribution at SnO2/C interface, which is believed to remarkably enhance interfacial polarization relaxation and contribute to microwave absorption. Our results illustrate that the ingenious integration of nanomaterials on carbon fiber cloth promises a way to achieve efficient and flexible microwave absorbers.

In this work, the (1-x)CaWO4-xNa2WO4 (x = 0.1, 0.2, denoted as 0.9CW-0.1NW and 0.8CW-0.2NW, respectively) ultralow-loss microwave dielectric ceramics were prepared via solid-state reaction method. Using low melting-point Na2WO4 as sintering aid to prepare CaWO4Na2WO4 composite ceramics, the sintering temperature of CaWO4 was successfully reduced while maintaining excellent microwave performance. The optimal microwave dielectric properties have been achieved at 900 °C for 0.9CW-0.1NW ceramic: εr = 9.0, Q × f = 105660 GHz, tanδ = 1.1 × 10−4 and τf = −35.4 ppm/°C at a frequency of 12.0 GHz. For the 0.8CW-0.2NW ceramic, the optimal microwave dielectric properties have been obtained at 740 °C, with εr = 8.5, Q × f = 97014 GHz, tanδ = 1.2 × 10−4 and τf = −37.4 ppm/°C at a frequency of 11.8 GHz. In summary, both composite ceramics exhibit low sintering temperatures, excellent dielectric properties and chemical compatibility with the Ag electrode. The findings of this study provide an effective approach to prepare novel composite ceramics as promising candidates for LTCC applications.

In the current research, we show that low-power state promotes variety-seeking. We argue that this effect occurs because consumers in low-power state desire control and variety-seeking as a way to restore sense of control. The effect of power on variety-seeking is reduced when consumer knowledge in a certain consumption domain is high (vs. low) because knowledge is an alternative way to retain sense of control. Three experiments provide systematic evidence for this effect across different product categories. These findings contribute to the literature on how variety-seeking can be used as a way to compensate and enhance our understanding of power and consumer knowledge.

Functionalization of endohedral metallofullerenes (EMFs) plays an important role in exploring the reactivity of EMFs and stabilizing missing EMFs, thus conferring tunable properties and turning EMFs into applicable materials. In this review, we present exhaustive progress on the functionalization of EMFs since 2019. Classic functionalization reactions include Prato reactions, Bingel–Hirsch reactions, radical addition reactions, carbene addition reactions, and so on are summarized. And new complicated multi-component reactions and other creative reactions are presented as well. We also discuss the structural features of derivatives of EMFs and the corresponding reaction mechanisms to understand the reactivity and regioselectivity of EMFs. In the end, we make conclusions and put forward an outlook on the prospect of the functionalization of EMFs.

Background Rotavirus infections, prevalent in human populations, are caused mostly by group A viruses. Immunization against rotaviruses in infancy is currently the most effective and economical strategy to prevent rotavirus infection. This study evaluated the safety of a novel hexavalent rotavirus vaccine and analyzed its dose and immunogenicity. Methods This randomized, double-blinded, placebo-controlled phase I clinical trial enrolled healthy adults, toddlers, and infants in Zhengding County, Hebei Province, northern China. 40 adults and 40 children were assigned in a 2:1:1 ratio to receive one vaccine dose, placebo 1, and placebo 2, respectively. 120 6–12 week old infants were assigned equivalently into 3 groups. The infants in each group were assigned in a 2:1:1 ratio to receive three doses of vaccine, placebo 1, and placebo 2, at a 28-day interval. Adverse events (AEs) until 28 days after each dose and serious adverse events (SAEs) until 6 months after the third dose were reported. Virus shedding until 14 days after each dose in infants was tested. Geometric mean concentrations (GMCs) and seroconversion rates were measured for anti-rotavirus IgA by using an enzyme-linked immunosorbent assay (ELISA). Results The solicited and unsolicited AE frequencies and laboratory indexes were similar among the treatment groups. No vaccine-related SAEs were reported. The average percentage of rotavirus vaccine shedding in the infant vaccine groups was 5.00%. The post-3rd dose anti-rotavirus IgA antibody geometric mean concentrations (GMC) and seroconversion rate were higher in the vaccine groups than in the placebo groups. Conclusions The novel oral hexavalent rotavirus vaccine was generally well-tolerated in all adults, toddlers and infants, and the vaccine was immunogenic in infants.

Heavy metal (HM) pollution in the marine environment has been of concern for decades. The potential impact of HMs carried by emerging marine pollutants such as microplastics (MPs) has attracted attention only in recent years. In this study, we investigated the single and combined chronic toxic effects (growth, burrowing behavior, and histopathology) of cadmium (Cd, three concentrations of low, medium, and high) and polystyrene (PS) microspheres (1 μm, 10 μg/L) on the coastal polychaete Perinereis aibuhitensis. Cd bioaccumulation under two exposure scenarios was also explored. Our results showed that Cd and PS did not affect worm growth for single or combined exposure, while 13.08 μg/L of Cd (CdL) alone significantly decreased the burrowing time of P. aibuhitensis in sediment. The presence of PS mitigated the hormetic effect of Cd on worm burrowing behavior, and this influence was Cd concentration-related. Cd body burdens in worms exposed to the medium and high Cd concentrations (CdM and CdH) were significantly greater than those of control worms with or without the presence of PS. In addition, Cd bioaccumulation was significantly higher with the coexistence of PS than those of Cd alone at the CdL and CdM groups. Histopathological analyses demonstrated a trend of epidermal and intestinal damages for single Cd/PS and their combined groups, and the contribution of PS-MPs should not be ignored. Our results indicate that the toxic effects and bioaccumulation pattern of Cd could be altered with the presence of PS-MPs for P. aibuhitensis, especially at environmentally relevant concentrations.

To mitigate the GFP-triggered artifacts in Fluorescence-detection Size-Exclusion Chromatography (FSEC), Jin et al. develop an FSEC assay utilizing nanobody technology which allows evaluation of the expression, monodispersity and thermostability of membrane proteins without the need for purification. It holds promise as a powerful tool for screening of membrane proteins for functional and structural studies.

A simulation model based on Dymola modelling was developed to investigate the dynamic characteristics of automatic generation control (AGC) for variable-load thermal power units in this study. Specifically, a 300 MW unit from a power plant in northern China was used to verify the model’s validity in steady-state processes and to analyze the behavior of the main thermal parameters under different rates of load changes. The economic performance of the unit under different rates of load changes is also analyzed by combining the economic indexes of “two regulations” in the power grid. Results indicate that as the rate of load changes increases, boiler output, main steam temperature, reheat steam temperature, main steam pressure, and working temperatures of various equipment fluctuate more intensely. Specifically, at a rate of load reduction of 2.0% Pe MW/min, the maximum deviation of the main steam temperature can reach 7.6 °C, with the screen-type superheater experiencing the largest heat exchange. To achieve a balance between safety and economics for the unit, the rate of load raising should not exceed 1.2% Pe MW/min, and the rate of load reduction should not exceed 0.8% Pe MW/min. This paper applies the covariance index and AGC assessment index of the thermal power unit load control system to the established dynamic simulation model to supplement the AGC assessment index in the “two regulations”, and to provide a flexible and reasonable system evaluation result for field operators to refer to, so as to improve the economics of the system on the basis of safety.

Micro and nanoplastics (MNPs) as emerging contaminants have become a global environmental issue due to their small size and high bioavailability. However, very little information is available regarding their impact on zooplankton, especially when food availability is a limiting factor. Therefore, the present study aims at evaluating the long-term effects of two different sizes (50 nm and 1 μm) of amnio-modified polystyrene (PS-NH2) particles on brine shrimp, Artemia parthenogenetica, by providing different levels of food (microalgae) supply. Larvae were exposed to three environmentally relevant concentrations (5.5, 55, and 550 μg/L) of MNPs over a 14-days of exposure with two food levels, high (3 × 105~1 × 107 cells/mL), and low (1 × 105 cells/mL) food conditions. When exposed to high food levels, the survival, growth, and development of A. parthenogenetica were not negatively affected at the studied exposure concentrations. By comparison, when exposed to a low food level, a U shape trend was observed for the three measured effects (survival rate, body length, and instar). Significant interactions between food level and exposure concentration were found for all three measured effects (three-way ANOVA, p < 0.05). The activities of additives extracted from 50 nm PS-NH2 suspensions were below toxic levels, while those from 1-μm PS-NH2 showed an impact on artemia growth and development. Our results demonstrate the long-term risks posed by MNPs when zooplankton have low levels of food intake.

Late-onset Alzheimer's disease (LOAD) is a common irreversible neurodegenerative disease with heterogeneous genetic characteristics. Identifying the biological biomarkers with the potential to predict the conversion from normal controls to LOAD is clinically important for early interventions of LOAD and clinical treatment. The polygenic risk score for LOAD (AD-PRS) has been reported the potential possibility for reliably identifying individuals with risk of developing LOAD recently. To investigate the external phenotype changes resulting from LOAD and the underlying etiology, we summarize the comprehensive associations of AD-PRS with multiple biomarkers, including neuroimaging, cerebrospinal fluid and plasma biomarkers, cardiovascular risk factors, cognitive behavior, and mental health. This systematic review helps improve the understanding of the biomarkers with potential predictive value for LOAD and further optimizing the prediction and accurate treatment of LOAD.

In practice, more and more companies are using warmth appeals in their advertisements, but not all warmth appeals can bring the expected results. Grounded in social perception, we propose that consumers’ inferences and behavioral intentions stemming from warmth appeals in advertising are moderated by brand concepts. Specifically, warmth appeal decreases competence inferences and, in turn, behavioral intentions toward the self-enhancement brands. However, it increases warmth inferences and, in turn, behavioral intentions toward self-transcendence brands. We tested our hypotheses through two experimental studies. Experiment 1 demonstrated that for self-enhancement brands, warmth appeals in advertisements decreased brand attitudes and purchase intentions; for self-transcendence brands, warmth appeals in advertisements increased brand attitudes and purchase intentions. Experiment 2 showed further evidence to the proposed effect and tested the mediating effects of warmth perception and competence perception. This research provides significant implications for advertising strategies.

Objective: To assess the immune effect of different types of hepatitis B vaccine (HepB) booster doses 2–32 years after primary immunization, explore the influencing factors, and offer guidance regarding the necessity and timing of boosters. Methods: In total, 1163 participants who were born from 1986 to 2015, received the HepB full-course primary vaccination, were hepatitis B surface antigen (HBsAg) and hepatitis B core antibody (anti-HBc) negative, and had hepatitis B surface antibody (anti-HBs)

Background: To assess the long-term protection conferred by plasma-derived hepatitis B vaccine at 20–31y after primary immunization during infancy in Chinese rural community. Method: Participants born between 1986 and 1996, who received a full course of primary vaccination with plasma-derived hepatitis B vaccine and had no experience with booster vaccination were enrolled. An epidemiological investigation was performed, and blood samples were collected to detect hepatitis B surface antigen (HBsAg), hepatitis B surface antibody (anti-HBs), and hepatitis B core antibody (anti-HBc). The positive rate of HBsAg, anti-HBs, and anti-HBc were calculated to evaluate the long-term protection of the plasma-derived hepatitis B vaccine. Results: A total of 949 participants were enrolled in the final analysis. Six subjects were detected to be HBsAg-positive, resulting in a HBsAg carrier rate of 0.63% (6/949). A total of 468 (52.41%) participants maintained a level of anti-HBs antibody ≥10 mIU/mL, with a GMC of 112.20 mIU/mL (95%CI: 97.72 ~ 128.82 mIU/mL). A significant downtrend was observed in the anti-HBs positive rate (P < .001). The average anti-HBc positive rate was 5.90% (56/949), increased with prolongation of immunization (P < .001). Conclusions: The plasma-derived hepatitis B vaccine maintained satisfactory protection at 20–31 y after primary immunization. These results indicate that a booster dose is not necessary. Further studies on the immune memory induced by the plasma-derived hepatitis B vaccine are needed.

Oil spill is a major marine environmental pollution issue. Research regarding the long-term effects of oil spills on the early life stage of marine fish is still limited. In this study, the potential adverse impact of crude oil from one oil spill accident which occurred in the Bohai Sea on the early life stages of marine medaka (Oryzias melastigma, McClelland, 1839) was evaluated. A 96-h acute test (larvae) and a 21-d chronic test (embryo–larvae) of water-accommodated fractions (WAFs) from crude oil were conducted, respectively. The results of the acute test showed that only the highest concentration of WAFs (100.00%) significantly affected the mortality of larvae (p < 0.01) and that the 96 h-LC50 was 68.92% (4.11 mg·L-1 expressed as total petroleum hydrocarbons (TPHs)). Larval heart demonstrated histopathological alterations in all WAF-exposed groups. The chronic test results showed that, except for larval mortality, the total hatching success (%)/hatching time of embryos in WAF treatments was not significantly different from those of the control group (p > 0.05), and no malformation was found in surviving larvae after 21 d of exposure. Nevertheless, the exposed embryos and larvae in the highest concentration of WAFs (60.00%) demonstrated significantly reduced heart rate (p < 0.05) and increased mortality (p < 0.01), respectively. Overall, our results indicated that both acute and chronic WAF exposures had adverse impacts on the survival of marine medaka. In the early life stages, the heart of the marine medaka was the most sensitive organ which showed both structural alteration and cardiac dysfunction.

Cadmium (Cd) pollution highly threatens food security and human health, and phytoremediation with Cd-tolerant plants is a cost-effective in situ method for remediation of Cd contamination. Broomcorn millet is known for its strong abiotic stress resistance and can be used as a pioneer crop in both marginal regions and newly reclaimed land. To evaluate their potential in remediation of Cd contamination, a total of 288 broomcorn millet core collections were investigated under hydroponic conditions to compare their capabilities in Cd tolerance, translocation, and accumulation. The core collections varied considerably in their growth parameters, Cd concentration, Cd translocation factor, Cd bioaccumulation factor, and Cd accumulation under Cd stress. According to the Cd tolerance index (TI) values, 160 varieties were Cd tolerant. The Cd TI was significantly positively correlated with Cd accumulation, and the shoot Cd concentrations of five Cd-tolerant varieties were more than 100 mgkg-1, the threshold for being Cd hyperaccumulators. Moreover, the concentrations of essential metal elements were significantly decreased in shoots, and Cd concentration had a significantly positive relationship with magnesium (Mg) and zinc (Zn) concentrations in roots under Cd stress. These results demonstrate that broomcorn millet shows considerable tolerance to Cd stress and great differences in Cd accumulation abilities among varieties. Accordingly, broomcorn millet is a promising plant species for Cd bioremediation, with valuable varieties that have been identified for further study on Cd tolerance mechanisms and the remediation of Cd contamination.

This research examines how recipients’ efforts to get out of the plight affect the empathy they evoke and the subsequent help they receive from donors. Through three experiments, we find that the higher the efforts made by the recipients to get out of the plight, the stronger the donors’ willingness to donate. This effect is moderated by the need severity of the recipients. The more serious the plight is, the stronger the impact of the degree of efforts on the willingness to donate. This research makes theoretical contributions to charitable giving and provides implications for non-profit organizations on describing recipients’ efforts to get out of their plight.

Alkyl-PAHs are the predominant form of PAHs in crude oils which are supposed to demonstrate different toxicities compared to non-alkyl PAHs. Little information is available about the toxicity of alkyl-PAHs on marine Artemia. This study addressed and compared the lethal, behavioral, growth and developmental toxicities of three alkyl-PAHs, namely 3-methyl phenanthrene (3-mPhe), retene (Ret) and 2-methyl anthracene (2-mAnt), to their non-alkyl forms, phenanthrene (Phe) and anthracene (Ant) using Artemia parthenogenetica (nauplii,

The exploration of safe antibiotic substitutes is one of the research hotspots in animal husbandry. Adding suitable plant essential oils into feed could improve the growth performance and immune capacity of animals. In order to make plant essential oil play a better role in feed application, sodium alginate and chitosan were used as the wall materials, and blended plant essential oils (BEO) as the core material to prepare BEO microcapsules by the sharp-hole condensation method. On the basis of single-factor experiments, the optimal preparation conditions for BEO microcapsules were obtained by response surface experiments. The physicochemical properties were characterized and analyzed by Fourier-transform infrared spectroscopy (FTIR) and field scanning electron microscope (FSEM). Meanwhile, the release mechanism was studied by simulating a gastrointestinal sustained-release experiment. The results showed that under the optimal preparation conditions, the encapsulation efficiency of BEO microcapsules could reach 80.33 ± 2.35%. FTIR and SEM analysis displayed that the microcapsules obtained had uniform color and size and a complete and compact structure. In vitro study indicated that the release amount of BEO microcapsules in the simulated intestinal fluid is higher than that in the simulated intestinal fluid, which was consistent with animal digestive and absorptive characteristics.

Abstract Although epitaxial strain imparted by lattice mismatch between a film and the underlying substrate has led to distinct structures and emergent functionalities, the discrete lattice parameters of limited substrates, combined with strain relaxations driven by film thickness, result in severe obstructions to subtly regulate electro‐elastic coupling properties in perovskite ferroelectric films. Here a practical and universal method to achieve highly strained phases with large tetragonal distortions in Pb‐based ferroelectric films through synergetic effects of moderately (≈1.0%) misfit strains and laser fluences during pulsed laser deposition process is demonstrated. The phase possesses unexpectedly large Poisson's ratio and negative thermal expansion, and concomitant enhancements of spontaneous polarization (≈100 µC cm−2) and Curie temperature (≈800 °C), 40% and 75% larger than that of bulk counterparts, respectively. This strategy efficiently circumvents the long‐standing issue of limited numbers of discrete substrates and enables continuous regulations of exploitable lattice states in functional oxide films with tightly elastic coupled performances beyond their present levels.

MgtE is a Mg2+ channel conserved in organisms ranging from prokaryotes to eukaryotes, including humans, and plays an important role in Mg2+ homeostasis. The previously determined MgtE structures in the Mg2+-bound, closed-state, and structure-based functional analyses of MgtE revealed that the binding of Mg2+ ions to the MgtE cytoplasmic domain induces channel inactivation to maintain Mg2+ homeostasis. There are no structures of the transmembrane (TM) domain for MgtE in Mg2+-free conditions, and the pore-opening mechanism has thus remained unclear. Here, we determined the cryo-electron microscopy (cryo-EM) structure of the MgtE-Fab complex in the absence of Mg2+ ions. The Mg2+-free MgtE TM domain structure and its comparison with the Mg2+-bound, closed-state structure, together with functional analyses, showed the Mg2+-dependent pore opening of MgtE on the cytoplasmic side and revealed the kink motions of the TM2 and TM5 helices at the glycine residues, which are important for channel activity. Overall, our work provides structure-based mechanistic insights into the channel gating of MgtE.

Background: Antibiotics are commonly used in the neonatal intensive care unit (NICU). The objective was to observe the effect of weekly antibiotic round in NICU (WARN) to the antibiotic use in NICU.Methods: A retrospective observational study was performed. Departmental-level diagnosis categories and the parameters of antibiotic usage in NICU for the period of 2016-2017 (Phase 1) and 2018-2019 (Phase 2) were collected. WARN in NICU was started since January 2018. A time series forecasting was used to predict the quarterly antibiotic use in Phase 2, based on data from Phase 1. The actual antibiotic use of each quarter in Phase 2 was compared with the predicted values.Results: Totally 9297 neonates were included (4743 in Phase 1, 4488 in Phase 2). The composition of the disease spectrum between Phase 1 and Phase 2 was not different (P > 0.05). In Phase 1 and Phase 2, the overall antibiotic rate was 94.4 and 74.2%, the average accumulative defined daily dose per month was 199.00 ± 55.77 and 66.80 ± 45.64, the median antibiotic use density per month was 10.31 (9.00-13.27) and 2.48 (1.92-4.66), the median accumulative defined daily dose per case per month was 0.10 (0.09-0.13) and 0.03 (0.02-0.47), the number of patients who received any kind of antibiotic per 1000 hospital days per month was 103.45 (99.30-107.48) and 78.66 (74.62-82.77), rate of culture investigation before antibiotics was 64 to 92%, respectively, and all were better than the predicted values (P < 0.01).Conclusion: The implementation of periodical antibiotic rounds was effective in reducing the antibiotics use in the NICU.

With the recent advance in chemical modification of fullerenes, electrosynthesis has demonstrated increasing importance in regioselective synthesis of novel fullerene derivatives. Herein, we report successively regioselective synthesis of stable tetra- and hexafunctionalized [60]fullerene derivatives. The cycloaddition reaction of the electrochemically generated dianions from [60]fulleroindolines with phthaloyl chloride regioselectively affords 1,2,4,17-functionalized [60]fullerene derivatives with two attached ketone groups and a unique addition pattern, where the heterocycle is rearranged to a [5,6]-junction and the carbocycle is fused to an adjacent [6,6]-junction. This addition pattern is in sharp contrast with that of the previously reported biscycloadducts, where both cycles are appended to [6,6]-junctions. The obtained tetrafunctionalized compounds can be successively manipulated to 1,2,3,4,9,10-functionalized [60]fullerene derivatives with an intriguing “S”-shaped configuration via a novel electrochemical protonation. Importantly, the stability of tetrafunctionalized [60]fullerene products allows them to be applied in planar perovskite solar cells as efficient electron transport layers.

Broomcorn millet (Panicum miliaceum L.) affected by smut (caused by the pathogen Sporisorium destruens) has reduced production yields and quality. Determining the tolerance of broomcorn millet varieties is essential for smut control. This study focuses on the differences in the phenotypes, physiological characteristics, and transcriptomes of resistant and susceptible broomcorn millet varieties under Sporisorium destruens stress. In diseased broomcorn millet, the plant height and stem diameter were reduced, while the number of nodes increased. After infection, the activities of superoxide dismutase and peroxidase decreased, and malondialdehyde and relative chlorophyll content (SPAD) decreased. Transcriptome analysis showed 514 and 5452 differentially expressed genes (DEGs) in the resistant and susceptible varieties, respectively. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of DEGs showed that pathways related to plant disease resistance, such as phenylpropanoid biosynthesis, plant–pathogen interaction, and plant hormone signal transduction, were significantly enriched. In addition, the transcriptome changes of cluster leaves and normal leaves in diseased broomcorn millet were analysed. Gene ontology and KEGG enrichment analyses indicated that photosynthesis played an important role in both varieties. These findings lay a foundation for future research on the molecular mechanism of the interaction between broomcorn millet and Sporisorium destruens.

Glycosylphosphatidylinositol (GPI) anchor modification is a posttranslational modification of proteins that has been conserved in eukaryotes. The biosynthesis and transfer of GPI to proteins are carried out in the endoplasmic reticulum. Attachment of GPI to proteins is mediated by the GPI-transamidase (GPI-TA) complex, which recognizes and cleaves the C-terminal GPI attachment signal of precursor proteins. Then, GPI is transferred to the newly exposed C-terminus of the proteins. GPI-TA consists of five subunits: PIGK, GPAA1, PIGT, PIGS, and PIGU, and the absence of any subunit leads to the loss of activity. Here, we analyzed functionally important residues of the five subunits of GPI-TA by comparing conserved sequences among homologous proteins. In addition, we optimized the purification method for analyzing the structure of GPI-TA. Using purified GPI-TA, preliminary single particle images were obtained. Our results provide guidance for the structural and functional analysis of GPI-TA.

Author summary MgtE is an Mg2+ transporter involved in Mg2+ homeostasis. Here, the authors report that ATP regulates the Mg+2-dependent gating of MgtE and use X-ray crystallography combined with functional studies to propose the molecular mechanisms involved in this process.

Elevated soil lead (Pb) concentrations are a global concern owing to the toxic effects of this heavy metal. Solidification/stabilization (S/S) of soils using reagents like Portland cement (PC) is a common approach for the remediation of Pb contaminated sites. However, it has been reported that under long-term field conditions, the performance of PC treatments can diminish significantly. Therefore, novel reagents that provide longer-term stabilization performance are needed. In this study, four magnesium oxide (MgO) products of different reactivity values were applied (5 wt%) to a Pb contaminated clayey soil. The short-term (1–49 days) and long-term (25–100 years) temporal stabilization effects were investigated by laboratory incubation and accelerated ageing methods, respectively. The concentration of Pb in Toxicity Characterization Leaching Procure (TCLP) leachate was ~14 mg/L for the untreated soil; ~1.8 times higher than the TCLP regulatory level (5 mg/L). Only one day after treatment with MgO, the leachate concentration was reduced to below the regulatory level (a reduction of 69.4%–83.2%), regardless of the MgO type applied. However, in the long-term accelerated ageing experiments, only treatments using the most reactive MgO type could provide leachate concentrations that were consistently below the TCLP threshold throughout the 100 years of simulated ageing. The soil treated with the MgO of lowest reactivity was the first to exceed the regulatory level, at simulated year 75. It is thus demonstrated that MgO reactivity has a significant effect on its long-term effectiveness for contaminated soil stabilization. This is attributed to differences in their specific surface area and readiness to carbonate, which may facilitate the immobilization of Pb in the long term. It is also noteworthy that compared to PC, reactive MgO is more environmentally friendly owing to lower energy consumption and reduced CO2 emissions during its manufacture. Keywords: Soil pollution, Lead contamination, Remediation, Reactive magnesia, Reactivity, Accelerated ageing

Cemented paste backfill (CPB) technology has been applied quite popular around the world. Yield stress is a key factor determining whether CPB could be transported. In order to reveal the effect of solid components on yield stress of CPB, a uniform design experimental program (four factors and six levels) was conducted to test the rheological property of a mine’s CPB. The tested four factors including mass fraction, cement versus other solids ratio, coarse tailings, and gravel contents were considered during the experiment design. Likewise, six experimental levels were given to each factor. Results of the test show that yield stress increased with the mass fraction and cement content. However, the trend reversed for the content of coarse tailings and gravel. Contribution of the four factors to yield stress in descending order is mass fraction > content of gravel > content of coarse tailings > cement versus other tailings ration. Effect of solid components on the yield stress of CPB is mainly due to the different flocculation structure inside the CPB. These various flow structures result in the different free-water content of CPB, leading to a different yield stress value.

Sedimentation of filling materials could cause pipe blocking accident in mines. However, few quantitative characterization studies have investigated the sedimentation characteristics of filling materials. In this study, the sedimentation property of iron tailings with a cement-sand ratio of 1 : 4 and mass concentration of 73%∼82% was investigated based on rheology measurements. Results showed that shear stress increased as shear rate rose from 0 s−1 to 120 s−1. The shear stress increased as the filling material concentration increased as well. However, when the shear rate was reversed from 120 s−1 to 0 s−1, the shear stress presented an increase-constant-decrease change pattern as the mass concentration increases in the rheological curve. Accordingly, the sedimentation performance of iron tailings filling material was divided into three types: intense sedimentation (the ascending rheological curve) in the mass concentration range of 73%∼76%, slight sedimentation (the constant rheological curve) in the mass concentration range of 77%∼79%, and almost no sedimentation (the descending rheological curve) in the mass concentration range of 80%∼82%. The associated mechanism involving slurry mass concentration-rheological curves-sedimentation performance was illustrated. A correlation between the pipeline rheology and filling material sedimentation performance was established, which provides a practical guide to avoid pipeline blocking while transporting the filling material.

Cemented paste backfill (CPB) is an economic and environmental friendly technique applied in underground mining for supporting surrounding rock and replacing the pillar. However, little is known about the temperature effects on CPB in mines having a large temperature fluctuation. The main purpose of this research was to investigate the effect of temperature change on the rheological properties (e.g., shear stress and apparent viscosity) of CPB with copper mine tailings. Specifically, a series of rheological tests were conducted on 6 CPB samples using a Brookfield R/S+ Rheometer under various temperatures (2°C, 10°C, 20°C, 30°C, 40°C, and 60°C). Our results showed that both shear stress and apparent viscosity of these tailing samples increased with temperature rising from 2 to 60°C. Likewise, temperature has a significant impact on the Bingham yield stress of thickened tailings. The yield stress decreased from 122 Pa (2°C) to 112 Pa (20°C) and then increased to 152 Pa (60°C). Moreover, the pipeline transport pressure drop of CPB at various temperatures was calculated, illustrating an obvious effect on the paste pipeline transport. Compared with 20°C, the pressure drop under 2°C and 60°C increased by 11% and 22%, respectively. The results of this study indicate that the temperature plays an essential role in determining rheological properties of CPB and its engineering application in mines particularly with naturally fluctuating temperatures.