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Made available in DSpace on 2021-06-25T12:16:52Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-05-21 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Changes in Brazilian corn production practices, have favored stink bugs, which feed on corn seedlings. Diceraeus melacanthus (Dallas) (Hemiptera: Pentatomidae), is a key pest in corn, and Euschistus heros (F.) (Hemiptera: Pentatomidae) is an emerging threat to corn. Economic thresholds (ET) for management of stink bugs are usually calculated based on the injury caused by adults. However, little is known about the impact of stink bug nymphs on corn seedlings. Thus, this work evaluated the effects of infestation with different nymphal instars on corn seedling weight (fresh and dry), plant height and stalk diameter. We also classified the level of overall injury to the seedlings using a rating scale. Corn seedlings in the V3 stage were infested with two insects of either D. melacanthus or E. heros in the 2(nd), 3(rd), 4(th), 5(th) instars or adult stage in separate greenhouse studies performed for each species. Plants without infestation were used as controls. Both species of stink bugs feeding on corn seedlings affected all the assessed parameters. The quantitative measures of seedling size (i.e., fresh weight, dry weight, plant height and stem diameter) were generally most severely affected by the 5(th) instars and adults of D. melacanthus and the 4(th) instars of E. heros. The qualitative injury rating scale generally showed a greater impact to seedlings by 3(rd) instar to adult life stages of each species. Across life stages, injury to seedlings caused by D. melacanthus was numerically greater than that from E. heros. These results will guide the development of a more accurate ETs for stink bug management on seedling corn plants. Sao Paulo State Univ, Sch Agr, Dept Crop Protect, BR-18610034 Botucatu, SP, Brazil Fed Univ Grande Dourados, Dept Entomol & Biodivers Conservat, BR-79804970 Dourados, MS, Brazil Agr Res & Rural Extens Co Santa Catarina, BR-89801970 Chapeco, SC, Brazil Univ Minnesota, Dept Entomol, St Paul, MN 55108 USA Sao Paulo State Univ, Sch Agr, Dept Crop Protect, BR-18610034 Botucatu, SP, Brazil CAPES: 001 CNPq: 303892/2016-1

Made available in DSpace on 2021-06-25T11:57:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-02-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The aim of this study was to evaluate different post-shock temperatures for tetraploid induction in the yellowtail tetra Astyanax altiparanae. Newly fertilized eggs were divided into four groups, three were submitted to heat shock (40 degrees C for 2 min) at 24 min post-fertilization (mpf) and another group remained without shock (control). Groups submitted to temperature shock were further separated at the following temperatures: 22 degrees C, 26 degrees C and 28 degrees C. Survival among embryonic development was counted and at hatching the ploidy was analyzed by flow cytometry. The results showed that the post-shock temperature affects the parameters analyzed and, therefore, must be considered for optimization of the production of tetraploid in A. altiparanae. Those data are innovative and could be used in future studies of basic biology in this species. Fed Univ Grande Dourados UFGD, BR-79825070 Dourados, MS, Brazil Sao Paulo State Univ, Aquacuiture Ctr, Via Acesso Prof Paulo Donato Castaane S-N, BR-14884900 Jaboticabal, SP, Brazil Chico Mendes Inst Biodivers Conservat, Natl Ctr Res & Conservat Continental Fish, Lab Fish Biotechnol, Rodovia Pref Euberto Nemesio Pereira de Godoy, BR-13630970 Pirassununga, SP, Brazil Sao Paulo State Univ, Aquacuiture Ctr, Via Acesso Prof Paulo Donato Castaane S-N, BR-14884900 Jaboticabal, SP, Brazil FAPESP: 2010/17429-1 FAPESP: 2011/11664-1 FAPESP: 2016/12383-0 CNPq: 471140/2012-0

Made available in DSpace on 2020-12-10T20:04:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-07-02 Fundacao de Apoio ao Desenvolvimento do Ensino, Ciencia e Tecnologia do Estado de Mato Grosso do Sul Candidayeast infections are the fourth leading cause of death worldwide. Peptides with antimicrobial activity are a promising alternative treatment for such infections. Here, the antifungal activity of a new antimicrobial peptide-PEP-IA18-was evaluated againstCandidaspecies. PEP-IA18 was designed from the primary sequence of profilin, a protein fromSpodoptera frugiperda,and displayed potent activity againstCandida albicansandCandida tropicalis, showing a minimum inhibitory concentration (MIC) of 2.5 mu M. Furthermore, the mechanism of action of PEP-IA18 involved interaction with the cell membrane (ergosterol complexation). Treatment at MIC and/or 10 x MIC significantly reduced biofilm formation and viability. PEP-IA18 showed low toxicity toward human fibroblasts and only revealed hemolytic activity at high concentrations. Thus, PEP-IA18 exhibited antifungal and anti-biofilm properties with potential applicability in the treatment of infections caused byCandidaspecies. Univ Fed Mato Grosso, Fac Pharmaceut Sci Food & Nutr, Prot Purificat Lab & Biol Funct, Campo Grande, MS, Brazil Fed Univ Grande Dourados, Ctr Biotechnol & Bioprospecting Studies Appl Meta, Dourados, MS, Brazil Sao Paulo State Univ, Inst Chem, Dept Biochem, Araraquara, SP, Brazil Univ Catolica Dom Bosco, Grad Program Biotechnol, S Inova Biotech, Campo Grande, MS, Brazil Univ Catolica Brasilia, Ctr Prote & Biochem Anal, Grad Program Genom Sci & Biotechnol, Brasilia, DF, Brazil Univ Brasilia, Fac Med, Grad Program Mol Pathol, Brasilia, DF, Brazil Sao Paulo State Univ, Inst Chem, Dept Biochem, Araraquara, SP, Brazil Fundacao de Apoio ao Desenvolvimento do Ensino, Ciencia e Tecnologia do Estado de Mato Grosso do Sul: 08/2015

Made available in DSpace on 2020-12-10T17:27:45Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-03-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Foundation for the Development of Education, Science and Technology of the State of Mato Grosso do Sul (FUNDECT) Federal University of Grande Dourados (UFGD) The identification of genes and polymorphisms in female zebu related to the leptin gene that may be involved in better productive and reproductive performances is relevant to the livestock and economic sector. Thus, the objective of this study was to evaluate the SNP markers (T954M and C305T) and the BM1500 microsatellite and to analyze their associations with the traits of body weight, age at first calving (AFC), calving interval (CI), and reproductive efficiency (RE) in Nellore heifers. DNA extraction was performed, followed by the amplification of all markers. Association analyses were carried out with each marker and each traits separately. The result showed that no significant association was found between the reproductive traits and all markers and between body weight and both SNP markers. For the BM1500 microsatellite, the effect of the allele 138 (p=0.0383) on body weight was observed, with the growth curve for this allele being related to the lower asymptotic weight. Therefore, the BM1500 microsatellite marker was the only one with a significant association with a productive trait providing information about the condition of body weight in Nellore heifers. Fed Univ Grande Dourados, Fac Biol & Environm Sci, Dourados Itahum Highway,Km 12, BR-79804970 Dourados, MS, Brazil Fed Univ Grande Dourados, Fac Agr Sci, Dourados Itahum Highway,Km 12, BR-79804970 Dourados, MS, Brazil Sao Paulo State Univ, Fac Agr & Vet Sci, Sao Paulo, Brazil Sao Paulo State Univ, Fac Agr & Vet Sci, Sao Paulo, Brazil

Made available in DSpace on 2021-06-25T11:50:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-02-04 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) The population discrimination and the classification of individuals have great importance for genetic improvement in population studies and genetic diversity conservation. Furthermore, multivariate approaches are often used, especially the Fisher and Anderson discriminant functions. New methodologies based on machine learning (ML) have shown to be promising for such procedures, but there is nonetheless a need for further evaluation and comparison of these methods. Thus, the present study evaluates the efficacy of supervised ML algorithms in classifying populations with different degrees of similarity-comparing them with discriminant analysis techniques proposed by Anderson and by Fisher. The methods of supervised ML tested were as follows: Naive Bayes, Decision Tree, k-Nearest Neighbors (kNN), Random Forest, Support Vector Machine (SVM) and Multi-layer Perceptron Neural Networks (MLP/ANN). To compare classification methods, we used phenotypic data of populations with different degrees of genetic similarity. Data stemmed from the genotypic information simulation for different populations submitted to the backcrossing scheme. Accuracy here means 30 repetitions from each classification method were compared by the Friedman and Nemenyi tests with a 95% confidence level. Classification methods based on machine learning algorithms showed superior results to the Fisher and Anderson discriminant functions, obtaining high accuracy where there was a higher similarity between populations. The kNN, Random Forest, SVM and Naive Bayes algorithms presented the highest accuracy, surpassing the Decision Tree algorithm and even MLP/ANN (which lost accuracy at a 96.88% similarity condition between populations). Thus, the present work confirms that ML techniques demonstrate greater accuracy in the discrimination and classification of populations without the limitations of statistical techniques. Univ Catolica Dom Bosco, Campo Grande, MS, Brazil Fed Univ Grande Dourados, Dourados, MS, Brazil Paulista State Univ Julio de Mesquita Filho, Ilha Solteira, SP, Brazil Univ Fed Mato Grosso do Sul, Campo Grande, MS, Brazil Fed Inst Educ Sci & Technol Mato Grosso do Sul, Campo Grande, MS, Brazil Univ Fed Mato Grosso do Sul, Inst Biosci, Lab Ecol & Evolutionary Biol, BR-79070900 Campo Grande, MS, Brazil Paulista State Univ Julio de Mesquita Filho, Ilha Solteira, SP, Brazil CAPES: PNPD/CAPES 88882.315120/2019-01 CAPES: CNPq 301840/2016-4

Made available in DSpace on 2021-06-25T11:51:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Fundacao Chapadao FUNDECT/MS (Fundacao de Apoio ao Desenvolvimento do Ensino, Ciencia e Tecnologia do Estado de Mato Grosso do Sul) Cover crops promote nutrient cycling, and lime and gypsum can alter the soil physical attributes. This study aimed to evaluate the effect of lime and gypsum rates applied to a no-tillage system with addition of residues of three cover crops on the soil physical attributes. This experiment was carried out in chapacrjo do sul-ms. The treatments were comprised of three cover crops (Urochloa ruziziensis, fallow, and Pennisetum glaucum), with gypsum (0, 2.3 and 4.6 Mg ha-1) and lime applied at a dose of 0, 2, 4, 6 Mg ha-1).The attributes evaluated were: soil density, macroporosity, microporosity, total porosity and penetration resistance. The soil of the experiment was classified an Oxisol. Cover crops and lime and gypsum improved macroporosity, microporosity and total porosity at all depths, 0-0.2 m. Millet presented lower values for penetration resistance with the lime application and without gypsum application. No residual effect on soil density was detected for lime and gypsum application or cover crops in the 0.1-0.2 m layer. Brazilian Cerrado producers will have a well-defined management system to follow aiming at improving the soil physical attributes. Sao Paulo State Univ, Postgrad Program Energy Agr, Botucatu, SP, Brazil Univ Fed Mato Grosso do Sul, Dept Agron, Chapadao Do Sul, MS, Brazil Fed Univ Grande Dourados, Postgrad Program Agron, Dourados, MS, Brazil Univ Estado Mato Grosso, Dept Agron, Alta Floresta, Mato Grosso, Brazil Sao Paulo State Univ, Postgrad Program Energy Agr, Botucatu, SP, Brazil FUNDECT/MS (Fundacao de Apoio ao Desenvolvimento do Ensino, Ciencia e Tecnologia do Estado de Mato Grosso do Sul): 35231.528.7972.06062016-2982

Made available in DSpace on 2021-06-25T11:50:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Sows are highly motivated to build a nest prior to farrowing, but, given the barren environment of farrowing crates, this behaviour is limited and improperly expressed. We aimed to assess the effects of a straw provision in the farrowing crate as environmental enrichment in the prepartum period on: 1) sows' reproductive and behavioural responses before, during and after farrowing, and 2) piglets' performance during lactation. Multiparous sows (N = 32) were assigned according to parity to two enrichment conditions: 1) Control sows, and 2) Straw sows, (N = 16 sows/treatment). In the control treatment, the sows were kept in farrowing crates with no environmental enrichment. In the straw treatment, straw was provided in the farrowing crate by purpose-built boxes attached to the crate bars next to the sows. Straw was provided 24 h prior to the expected due date and removed after farrowing. Sows' behaviours (N = 12 sows/treatment) were recorded uninterruptedly from 12 h prior to farrowing until the 13th day of lactation. Data on reproductive performance (number of liveborn and stillborns, farrowing duration, and births interval) were collected. Five piglets were selected from each sow for assessment of performance and levels of blood lactate. All piglet deaths, as well as their causes, were recorded. Sows from straw treatment exhibited a higher frequency of nest-building behaviour and lower frequency of stereotyped behaviour and sitting posture (P < 0.05). No differences were found in the sows' behaviours during farrowing (P > 0.05). The sows from control treatment exhibited a higher number of posture changes (P = 0.057). The frequency of nursing was higher in the sows that had access to straw (P < 0.05). No differences were observed in reproductive performance (P> 0.05). Providing straw had no effects on performance, lactate levels, or postnatal mortality of piglets (P > 0.05). Providing nesting material in the farrowing crate improves the welfare of sows by increasing their motivation to build the nest and reducing the stereotyped behaviours prior to farrowing. Moreover, access to straw reduces sow unrest during farrowing and stimulates the maternal behaviour of nursing. Fed Univ Grande Dourados, Coll Agr Sci, Dourados, MS, Brazil Univ Fed Mato Grosso do Sul, Dept Anim Sci, Campo Grande, MS, Brazil Univ Ctr Grande Dourados, Sch Vet Med, Dourados, MS, Brazil S ao Paulo State Univ, Sch Agr & Veterinarian Sci, Jaboticabal, SP, Brazil Sao Paulo State Univ, Coll Vet Med & Anim Sci, St Prof Doctor Walter Mauricio Correa W-N, Botucatu, SP, Brazil Univ Fed Rural Rio de Janeiro, Dept Anim Nutr & Pastures, Seropedica, RJ, Brazil Sao Paulo State Univ, Coll Vet Med & Anim Sci, St Prof Doctor Walter Mauricio Correa W-N, Botucatu, SP, Brazil

Background Children with Developmental Coordination Disorder (DCD) are known to have poor physical fitness and psychosocial problems. The autonomous nervous system (ANS) plays an essential role in the regulation of human neurophysiological processes. Inadequate ANS modulation has been associated with harmful health conditions such as poor aerobic power, high body mass index, and symptoms of stress and anxiety. Modulation of ANS in children with DCD needs to be further investigated taking into consideration variables that may influence its function. For instance, would the level of physical fitness or the symptoms of stress and anxiety affect the ANS modulation of children with DCD? Aims To examine the ANS modulation during supine and standing postures, and stress/anxiety with questionnaire data from children with probable-DCD (p-DCD). Methods and procedures: Thirty children, 8–12 years old, composed two groups paired by age, gender, peak volume of oxygen uptake (aerobic power), and body mass index (BMI): p-DCD (9 boys, mean age 10.8 y) and typically developing (TD). Both groups were compared for stress/anxiety assessment by questionnaire and spectral, symbolic, and complexity heart rate variability (HRV) analyses during posture changes. Outcomes and results p-DCD group showed higher stress symptoms than TD group for stress/anxiety assessment in the questionnaire's data, but HRV analyses showed no differences between the two groups. Both groups showed parasympathetic prevalence during supine posture and sympathetic prevalence during standing posture. Conclusions and implications Children with p-DCD had similar autonomic control function as TD children during posture change (supine to standing). Symptoms of stress and anxiety demonstrated by p-DCD did not impact their ANS modulation. These results indicate that aerobic power and BMI are probable protective factors of ANS modulation for these children., Developmental coordination disorder (DCD); Autonomous nervous system; Heart rate variability; Stress and anxiety.

Made available in DSpace on 2021-06-25T12:21:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-10-13 Foundation for Support to the Development of Education, Science and Technology of Mato Grosso do Sul State (FUNDECT) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The indiscriminate use of synthetic insecticides has led to the emergence of resistant populations ofPlutella xylostella L. (Lepidoptera: Plutellidae), hindering its control and causing economic damage to brassica production. Thus, in order to seek control alternatives, this study aimed at assessing the bioactivity of the ethanolic extracts ofSchinus terebinthifolius(Anacardiaceae),Annona coriacea(Annonaceae),Annona crassiflora(Annonaceae), andSerjania marginata(Sapindaceae) on the life cycle ofP. xylostella. Cabbage leaf discs were immersed in the treatments at a concentration of 10mg/mL and given daily to the larvae. Larval and pupal duration and survival, pupae weight, the longevity of males and females, number and survival of eggs, incubation period, and days of oviposition parameters were evaluated. All extracts differed significantly from the control in terms of larval and pupal duration, pupal survival, and pupae weight. All the extracts prolonged the larval and pupal period ofP. xylostella. OnlyA. coriaceadid not differ significantly from the control in terms of larval survival, and the highest larval mortality was observed in the treatment withS. marginata(50%). Pupal survival, pupae mass, oviposition period, and egg survival were reduced in all treatments with ethanolic extracts. The treatments withAnnonaandS. marginataspecies have reduced the number of eggs. Treatments withS. terebinthifoliusandS. marginatahave reduced the incubation time of eggs. Thus, the treatments with ethanolic plant extracts act on the life cycle ofP. xylostellanegatively impacting the biological parameters evaluated. Fed Univ Grande Dourados, Fac Biol & Environm Sci, Lab Insect Plant Interact, Rodovia Dourados Itahum,Km 12, Dourados, MS, Brazil Fed Univ Grande Dourados, Fac Agr Sci, Lab Vegetables Prod, Rodovia Dourados Itahum,Km 12, Dourados, MS, Brazil Fed Univ Grande Dourados, Biol Control Lab, Fac Biol & Environm Sci, Rodovia Dourados Itahum,Km 12, Dourados, MS, Brazil Sao Paulo State Univ, Fac Agron Sci, Dept Vegetal Prod, Phytosanit Def,Lab Social Insects Pest, Rua Jose Barbosa Barros, BR-18610307 Botucatu, SP, Brazil Fed Univ Southern Bahia UFSB, Technosci & Innovat Training Ctr, Technosci & Innovat Training Ctr CFTCi, Itabuna Access Highway,Km 39, Itabuna, Bahia, Brazil Sao Paulo State Univ, Fac Agron Sci, Dept Vegetal Prod, Phytosanit Def,Lab Social Insects Pest, Rua Jose Barbosa Barros, BR-18610307 Botucatu, SP, Brazil Foundation for Support to the Development of Education, Science and Technology of Mato Grosso do Sul State (FUNDECT): 71/711.130/2018 CNPq: 304302/2015-5

Made available in DSpace on 2021-06-25T11:21:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-09-18 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Introduction The emergence of myopathies such as wooden breast in the poultry industry generally associated with the fast development of the breast muscle of broilers has provided changes in the morphological structure of muscle tissues, as well as problems of meat qualitative attributes. The aim of this study was to evaluate physical, chemical, qualitative, and sensorial attributes of broiler fillets associated with severity levels of wooden breast (WB) myopathy in a poultry slaughterhouse. Materials and methods Three hundred fillets in a poultry slaughterhouse were classified into three severity levels: normal (100 samples), moderate (100 samples), and severe (100 samples). Results: After identification, classification, and description of changes, fillets with a severe WB level presented higher levels of red (a*), yellow (b*), and final pH. The lowest shear force and the highest myofibrillar fragmentation index were observed in fillets with a severe level when compared with normal fillets. The collagen content increased according to severity level. Tasters better evidenced the characteristic taste of chicken meat when tasting fillets with a severe WB level when compared with normal and moderate fillets. The succulence and preference of the Brazilian testers increased according to the severity level of the myopathy. Conclusion In general, fillets with moderate and severe WB myopathy were affected not only in appearance but also in qualitative, physical, chemical, and sensory characteristics. Sao Paulo State Univ, Coll Vet Med & Anim Sci, St Prof Doctor Walter Mauricio Correa W-N, Botucatu, SP, Brazil Fed Univ Grande Dourados, Coll Agr Sci, Km 12, Dourados, MS, Brazil Fed Univ South & Southeast Para, Inst Studies Humid Trop, St Alberto Santos Dumont W-N, Xinguara, Para, Brazil Sao Paulo State Univ, Coll Vet Med & Anim Sci, St Prof Doctor Walter Mauricio Correa W-N, Botucatu, SP, Brazil CAPES: 001

Made available in DSpace on 2019-10-04T12:31:01Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-10-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) FUNDECT Federal University of Grande Dourados One Pr(III) lanthanide ion complex was initially synthesized and characterized by TGA-DSC in air atmosphere, as well as characterized by CHN elemental analysis, defining the stoichiometric ratio as Pr(DMBz)(3). The gaseous products evolved during the thermal decomposition were also monitored in N-2 atmosphere employing TGA/FT-IR system. A crystal structure is obtained by state-of-the-art powder X-rays diffraction methods measured in conventional laboratory equipment and refined by the Rietveld method, which defined it as a monoclinic system of the space group P2(1)/c with a polymeric crystal structure, [Pr(DMBz)(3)](n). FT-IR theoretical spectrum and time-dependent density functional theory (TD-DFT) were calculated from TGA-DSC and crystalline system data. The experimental and theoretical FT-IR spectra present a high correlation degree when the main stretching bands are compared, while the energy transfer (HOMO - LUMO) in their neighborhoods suggests the main contributions of the light-emitting states. (C) 2018 Published by Elsevier B.V. on behalf of Chinese Society of Rare Earths. Fed Univ Grande Dourados, CP 364, BR-79804970 Dourados, MS, Brazil State Univ Sao Paulo, Inst Chem, BR-14801970 Araraquara, SP, Brazil Univ Fed Mato Grosso do Sul, Chem Inst, BR-79074460 Campo Grande, MS, Brazil State Univ Sao Paulo, Chem Dept, BR-17033360 Bauru, SP, Brazil Univ Fed Juiz de Fora, BR-36036330 Juiz De Fora, MG, Brazil State Univ Sao Paulo, Inst Chem, BR-14801970 Araraquara, SP, Brazil State Univ Sao Paulo, Chem Dept, BR-17033360 Bauru, SP, Brazil CNPq: 474259/2013-7 FUNDECT: 23/200.632/2014

Made available in DSpace on 2018-11-26T16:04:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-04-01 Unconventional approaches can be used control populations of disease-carrying insect to improve health, economic, social, and environmental standards. There is a need for prolonged reduction of vector populations, using low-toxicity and low-cost products in domestic settings. Metals such copper and iron have shown to be toxic to insect larvae, with a delay in reproduction, damage to the cells of the digestive system and the production of free radicals and oxidant species, resulting in tissue damage and death. To control insect breeding, we synthesized and characterized Fe (III) acetate via carbonate. Fe (III) acetate showed no toxic effect on mosquito larvae up to 1.000 mg L-1, but was bactericidal for Gram-positive and Gram-negative bacteria, mainly due to the action of Fe (III) acetate. Sao Paulo State Univ, Inst Chem, BR-14800060 Araraquara, SP, Brazil Univ Fed Mato Grosso do Sul, Inst Chem, BR-79074460 Campo Grande, MS, Brazil Fed Univ Grande Dourados, Fac Exact Sci & Technol, Dept Chem, Dourados Itahum Highway 12 Km,POB 533, BR-79804970 Dourados, MS, Brazil Univ Estadual Campinas, Inst Biol, Dept Anim Biol, POB 6109,Monteiro Lobato St, BR-13083862 Campinas, SP, Brazil Sao Paulo State Univ, Inst Chem, BR-14800060 Araraquara, SP, Brazil

Made available in DSpace on 2018-11-26T17:41:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-12-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Suzano Pulp and Paper Company S.A. Fertilization of Eucalyptus plantations using sewage sludge on unfertile tropical soils represents an alternative to using mineral N and P fertilizers. A 44-month field experiment was conducted to study the effects of increasing application of sludge, and its interactions with mineral N and P fertilizers, on wood volume. Four rates of sludge (0, 8,15 and 23 Mg ha(-1), dry base), N (0, 47, 95 and 142 kg ha(-1)) and P (0, 28, 56 and 84 kg ha(-1) of P2O5) were combined in a 4 x 4 x 4 factorial scheme in a totally randomized block design. Response surface and age-shift modeling was used to establish an initial recommendation for mineral fertilization of the Eucalyptus plantations treated with sludge and to analyze the implications of increased growth on the duration of the forest cycle in a tropical climate. The results showed that from 8 to 44 months after planting, the sludge application (with or without N and P) yielded a statistically larger wood volume (P < 0.05), compared to application of N and P fertilizers only. The response surface modeling showed the following outcomes: i) application of sludge based on N criterion reduced the need for N and P fertilizers by 100%; and ii) an increase in wood volume by 7% could be achieved, compared to NPK fertilizers only, if 2/3 of the recommended P was applied. The cultivation time to produce 150 m(3) ha(-1) of wood volume was 45 months for the control and was reduced by two, three, four, or five months, respectively, through application of recommended P, sludge dose, sludge plus one third of P, and sludge plus two thirds of P. On the whole, sewage sludge could represent an excellent unconventional N and P fertilizer source for wood production on unfertile tropical soils. (C) 2017 Elsevier Ltd. All rights reserved. Univ Sao Paulo, Dept Plant Nutr, Ctr Nucl Energy Agr, BR-13400970 Piracicaba, SP, Brazil Fed Univ Grande Dourados, Sch Engn, BR-79804070 Dourados, MS, Brazil EMBRAPA Cerrado, BR-73310970 Planaltina, DF, Brazil Univ Sao Paulo, Luiz de Queiroz Coll Agr, BR-13418900 Piracicaba, SP, Brazil Suzano Papel & Celulose SA, BR-18200000 Itapetininga, SP, Brazil Univ Florida, Inst Food & Agr Sci, Indian River Res & Educ Ctr, Ft Pierce, FL 34945 USA Sao Paulo State Univ, Sch Engn, Dept Plant Protect Rural Engn & Soils, BR-15385000 Ilha Solteira, SP, Brazil Univ Sassari, Dipartimento Architettura Design & Urbanist, Via Colombo 1, I-08100 Nuoro, Italy Univ Sassari, Desertificat Res Ctr, Viale Italia 39, I-07100 Sassari, Italy Sao Paulo State Univ, Sch Engn, Dept Plant Protect Rural Engn & Soils, BR-15385000 Ilha Solteira, SP, Brazil FAPESP: 2004/15097-0 FAPESP: 2015/09690-5 CNPq: 313374/2014-7

Made available in DSpace on 2018-11-26T17:42:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-11-01 Locomotion issues in broiler production may decrease performance (carcass yield and traits) and lead to high financial losses. This study evaluates the addition of glucosaminoglycans in broiler diets to minimize the lack of proper bone development and joint weakening. The experiment was conducted using 2,160 broilers randomly distributed in a factorial pattern (3 x 3) using 3 levels of glucosamine sulfate (0, 0.12, and 0.24%) and 3 levels of chondroitin sulfate addition (0, 0.08, and 0.16%). Eight repetitions were used for each treatment, distributed in 72 pens with 30 broilers each. There was a quadratic effect on feed conversion for broilers from 1 to 42 d old (P = 0.0123) for the addition of chondroitin, and better feed conversion was obtained by adding 0.08% of chondroitin. The relative tibia weight, the width of the proximal epiphysis and diaphysis presented a linear increased effect in broilers at 42 d old. An interaction was found between the amount of chondroitin x glucosamine and the number of chondrocytes in the proximal cartilage of the tibia (P = 0.0072). There was a quadratic effect of glucosamine levels (P = 0.0107) in the birds that had received the 0.16% addition of chondroitin, and the presence of 0.18% glucosamine increased the number chondrocytes in the cartilage of broilers. These results provide the first evidence that broilers may benefit from increased dietary chondroitin sulfate. These results indicate that the addition of glucosamine and chondroitin sulfates in broiler feed rations might alleviate leg conditions and decrease financial losses in the broiler industry. Brazil Univ, Descalvado, SP, Brazil Sao Paulo State Univ, Dept Morphol & Anim Physiol, Jaboticabal, SP, Brazil Fed Univ Grande Dourados, Coll Agr Sci, Dourados, MS, Brazil Sao Paulo State Univ, Dept Bioengn, Ribeirao Preto, SP, Brazil Sao Paulo State Univ, Dept Morphol & Anim Physiol, Jaboticabal, SP, Brazil Sao Paulo State Univ, Dept Bioengn, Ribeirao Preto, SP, Brazil

Made available in DSpace on 2019-10-04T12:14:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-10-01 A total of 172 pigs were randomly allocated to two stunning treatments: carbon dioxide (CO2) and electrical stunning at a commercial abattoir. The Longissimus lumborum (LL) muscle was used to evaluate the effects of stunning methods on pork quality and financial losses of carcasses and viscera condemnation was also determined following by sanitary inspection. There was no effect due to stunning method on quality parameters (P > .137) of pH 24, drip or thaw loss, lipid oxidation, myofibrillar fragmentation index, shear force or PSE / DFD meat incidence. The LL muscle of the electrically stunned pigs had a higher L * value (P < .05), higher cooking loss (P < .001) and lower pH 45 (P < .05) compared to those stunned with CO2. CO2 stunned pigs had fewer carcasses condemned due to fractures / lesions (P < .05), hepatic and renal congestion (P < .05). CO2 stunning can be beneficial from an economic point of view because it has reduced losses through condemnation at the slaughterhouse. Fed Univ Grande Dourados, Coll Agr Sci, BR-79804970 Dourados, MS, Brazil Univ Estadual Paulista, Fac Vet Med & Anim Sci, BR-18618970 Botucatu, SP, Brazil Fdn Univ Rio Grande Sul, BR-96203900 Rio Grande, RS, Brazil Univ Estadual Paulista, Fac Vet Med & Anim Sci, BR-18618970 Botucatu, SP, Brazil

Made available in DSpace on 2020-12-10T16:57:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-10-13 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Agence Universitaire de la Francophonie (PRISA) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Linkage disequilibrium (LD) plays an important role in genomic selection and mapping of quantitative trait loci (QTL). This study investigated the pattern of LD and effective population size (N-e) in Gir cattle selected for yearling weight. For this purpose, 173 animals with imputed genotypes (from 18 animals genotyped with the Illumina BovineHD BeadChip and 155 animals genotyped with the Bovine LDv4 panel) were analysed. The LD was evaluated at distances of 25-50 kb, 50-100 kb, 100-500 kb and 0.5-1 Mb. The N-e was estimated based on 5 past generations. The r(2) values (a measure of LD) were, respectively, .35, .29, .18 and .032 for the distances evaluated. The LD estimates decreased with increasing distance of SNP pairs and LD persisted up to a distance of 100 kb (r(2) = .29). The N-e was greater in generations 4 and 5 (24 and 30 animals, respectively) and declined drastically after the last generation (12 animals). The results showed high levels of LD and low N-e, which were probably due to the loss of genetic variability as a consequence of the structure of the Gir population studied. Sao Paulo State Univ, Fac Agr & Vet Sci, Genet Anim Breeding, Jaboticabal, SP, Brazil Sao Paulo State Univ, Fac Vet Med & Anim Sci, Dept Breeding & Anim Nutr, Botucatu, SP, Brazil Inst Anim Sci, Ctr Beef Cattle, Sertaozinho, Brazil Fed Univ Grande Dourados, Fac Agr Sci, Dourados, Brazil Sao Paulo State Univ, Fac Agr & Vet Sci, Genet Anim Breeding, Jaboticabal, SP, Brazil Sao Paulo State Univ, Fac Vet Med & Anim Sci, Dept Breeding & Anim Nutr, Botucatu, SP, Brazil FAPESP: 2015/06686-7 Agence Universitaire de la Francophonie (PRISA): 1140/2018 CNPq: 304047/2017-1

Made available in DSpace on 2020-12-10T16:59:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-10-22 Foundation for the Support to the Development of Teaching, Science and Technology of the State of Mato Grosso do Sul (Fundect) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) The increase in haylage production leads to the search for additives that improve its fermentation and nutritional value. This study aimed to assess the effect of adding crude glycerine and microbial additives on losses, fermentation parameters and nutritional value of haylage. The treatments were composed of three doses of crude glycerine (0, 60 and 120 g/kg forage) and three types of inoculation (control (distilled water), SIL (Lactobacillus plantarum 2.6x10(10) CFU/g and Pediococcus pentosaceus 2.6x10(10) CFU/g) and INC (Bacillus subtilis 2.0x10(9) CFU/g, Lactobacillus plantarum 8.0x10(9) CFU/g and Pediococcus acidilactici 1.0x10(10) CFU/g)). A negative linear effect was observed in the fibre fraction contents of the haylages as a function of crude glycerine addition, which contributed to similarly increasing dry matter in vitro digestibility coefficients. The use of inoculants also resulted in haylages with higher digestibility coefficients of 635.1 and 646.8 g/kg dry matter (DM) in the treatments inoculated with INC and SIL, respectively. Fermentation losses were reduced by adding crude glycerine and were not impacted by the microbial inoculants. Higher lactic acid productions were obtained as a function of crude glycerine doses. Acetic acid productions decreased from 29.3 g/kg DM to 19.2 g/kg DM between crude glycerine doses of 0 and 120 g/kg forage, respectively. SIL led to the highest lactic acid productions compared to INC and the control. Crude glycerine improves the fermentation parameters and nutritional value of haylages. However, the microbial inoculants had little impact on the parameters assessed. Fed Univ Grande Dourados, Coll Agr Sci, BR-79804970 Dourados, MS, Brazil Sao Paulo State Univ, Coll Vet Med & Anim Sci, BR-14884900 Jaboticabal, SP, Brazil Sao Paulo State Univ, Coll Vet Med & Anim Sci, BR-14884900 Jaboticabal, SP, Brazil Foundation for the Support to the Development of Teaching, Science and Technology of the State of Mato Grosso do Sul (Fundect): 0239/2014

Made available in DSpace on 2018-11-26T16:33:01Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-01-01. Added 1 bitstream(s) on 2021-07-15T15:01:26Z : No. of bitstreams: 1 S1516-635X2016000100125.pdf: 112453 bytes, checksum: 73f42cb188462e446fadb30d3b15dee0 (MD5) This study evaluated the effects of the dietary inclusion of an enzyme blend and a direct-fed microbials in broiler diets on litter production and quality. In total, 900 Cobb 500 (R) broiler chicks were distributed according to a completely randomized design into 4 treatments and 9 replicates of 25 birds each. Broilers were reared from 1 to 42 days of age. The treatments consisted of the following diets: NC: negative control; DFM: NC + 500 ppm of direct-fed microbials product (DFM), containing Bacillus subtilis and Bacillus licheniformis; ENZ: diet formulated with an enzyme blend (20 ppm phytase, 200 ppm protease and 200 ppm of xylanase); DFM+E: ENZ + DFM. Birds and litter were weighed at the start and end of the rearing period, for litter production and waste ratio (Rw) determination. Litter samples were analyzed for dry matter (DM) content, total and thermotolerant coliform counts, nutrient composition (nitrogen (N), phosphorous (P) and potassium (K)), and fiber fraction (neutral detergent fiber (NDF), acid detergent fiber (ADF) and lignin). The dietary inclusion of the evaluated additivesdid not influence litter production or Rw; however, ADF (%), NDF (kg and kg/kg DM litter), and total and thermotolerant coliform counts were reduced, and N content increased in the litter. The diets containing enzymes (ENZ and DFM+ E) reduced litter P content. The addition of exogenous enzymes and their combination with a DFM based on Bacillus spp. Did not affect waste production, and reduced litter microbial load, and the contents of P and insoluble fiber in the litter. Sao Paulo State Univ, Dept Nutr & Anim Prod, Pirassununga Ave Duque de Caxias 225, BR-13635900 Pirassununga, SP, Brazil Sao Paulo State Univ, Dept Agr Engn, BR-14884900 Jaboticabal, SP, Brazil Sao Paulo State Univ, Dept Morphol & Anim Physiol, BR-14884900 Jaboticabal, SP, Brazil DSM Nutr Prod, BR-05321010 Sao Paulo, SP, Brazil Fed Univ Grande Dourados, Coll Agr Sci, BR-79804970 Dourados, MS, Brazil Sao Paulo State Univ, Dept Nutr & Anim Prod, Pirassununga Ave Duque de Caxias 225, BR-13635900 Pirassununga, SP, Brazil Sao Paulo State Univ, Dept Agr Engn, BR-14884900 Jaboticabal, SP, Brazil Sao Paulo State Univ, Dept Morphol & Anim Physiol, BR-14884900 Jaboticabal, SP, Brazil

Made available in DSpace on 2020-12-10T17:29:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-05-23 Diretoria Executiva de Gestao da Pesquisa e da Pos-Graduacao (DEGPP, UNIPAR) Fundacao de Apoio ao Desenvolvimento do Ensino, Ciencia e Tecnologia do Estado de Mato Grosso do Sul (FUNDECT) Ethnopharmacological relevance: Baccharis trimera (Less.) DC is a perennial subshrub, popularly known as carqueja, that belongs to the Asteraceae family. Ethnobotanical studies indicate that this species is used for the treatment of diabetes and digestive and liver diseases. However, studies that sought to validate its popular use were conducted using ethanolic extracts of the plant, which does not reflect the ethnomedicinal use of this species in humans. Aim of the study: Non-alcoholic fatty liver disease (NAFLD) is characterized by triglyceride accumulation in the liver that can progress to cirrhosis and hepatocellular carcinoma. Because of the severity of this disease, less toxic and more effective therapeutic agents need to be developed. B. trimera may be a promising therapeutic alternative, but its activity against multiple risk factors for liver disease (e.g., smoking, dyslipidemia, and diabetes mellitus) has not been studied. The present study investigated the effects of an ethnomedicinal form of a B. trimera preparation in a rat model of NAFLD that is associated with multiple risk factors. Material and methods: Phytochemical analysis of the ethanolic soluble fraction of B. trimera extract was performed using ultra-performance liquid chromatography coupled to high-resolution mass spectrometry. Streptozotocin was used to induce diabetes in male Wistar rats. The rats received a 0.5% cholesterol-enriched diet and were exposed to cigarette smoke (9 cigarettes/day, 5 days/week, for 4 weeks). In the last 2 weeks, the animals were orally treated with vehicle (negative control group), B. trimera extract (30, 100, and 300 mg/kg), or insulin + simvastatin. One group of rats that was not exposed to these risk factors was also evaluated. Blood was collected for glucose, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) analysis. The liver and feces were collected for lipid quantification. The liver was additionally processed for histopathological analysis. Results: The model successfully induced NAFLD and increased levels of glucose, AST, and ALT in the negative control group. Treatment with the B. trimera extract (30 and 100 mg/kg) and insulin + simvastatin decreased hepatic and fecal lipids. In contrast to insulin + simvastatin treatment, all three doses of B. trimera effectively reduced AST and ALT levels. Conclusion: B. trimera may be promising as a hepatoprotective agent against hepatic lesions that are caused by multiple risk factors. Univ Paranaense, Lab Preclin Res Nat Prod, Postgrad Program Med Plants & Phytotherapeut Basi, Umuarama, PR, Brazil Univ Paranaense, Nursing Dept, Francisco Beltrao, PR, Brazil Assis Gurgacz Fac, Lab Prevent & Diag, Cascavel, PR, Brazil Sao Paulo State Univ, Lab Genet & Cell Therapy, Assis, SP, Brazil Fed Univ Grande Dourados, Fac Hlth Sci, Lab Electrophysiol & Cardiovasc Pharmacol, Rodovia Dourados Itahum,Km 12,POB 533, BR-79804970 Dourados, MS, Brazil Univ Fed Parana, Lab Pharmacol & Metab, Postgrad Program Pharmacol, Curitiba, Parana, Brazil Pequeno Principe Fac, Inst Res Pele Pequeno Principe, Curitiba, Parana, Brazil Sao Paulo State Univ, Lab Genet & Cell Therapy, Assis, SP, Brazil Fundacao de Apoio ao Desenvolvimento do Ensino, Ciencia e Tecnologia do Estado de Mato Grosso do Sul (FUNDECT): 59/300351/2016

Made available in DSpace on 2019-10-04T12:41:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01. Added 1 bitstream(s) on 2021-07-15T15:04:57Z : No. of bitstreams: 1 S1516-635X2019000200312.pdf: 737561 bytes, checksum: 2c2cc7533656e83eab92ca582075faf5 (MD5) Trade liberalization during the last decades has increased among nations as a result of decreased tariffs. However, during the same period, market agents have created new protection measures to replace such mechanisms in an attempt to promote the domestic economic stability of countries, while expanding and ensuring the trade between nations. Considering that Brazil is the second largest broiler producer and chicken-meat exporter worldwide, the present study proposed to analyze the main barriers imposed on Brazilian chicken meat imports by the ten major importing countries. This is a documentary research with primary and secondary data, of a descriptive-exploratory nature, under a qualitative approach. Semi-structured questionnaires including open-and close-ended questions were sent using Survey Monkey software to the Brazilian Association of Animal Protein. The study revealed that technical requirements are those most often imposed by the importing countries, indicating their concern about the safety, quality, and origin of the imported good. Commercial requirements do not constitute a problem for the growth of Brazilian chicken meat exports; however, the requirements made by some countries preclude the exports. Fed Univ Grande Dourados, Coll Agr Sci, Km 12,Rodovia Dourados Itanhum, BR-79804970 Dourados, MS, Brazil Brazil Univ, 950 Ave Hilario da Silva, BR-13690970 Descalvado, SP, Brazil Sao Paulo State Univ, Dept Morphol & Anim Physiol, Via Acesso Prof Paulo Donato Castelane, BR-14884900 Jaboticabal, SP, Brazil Sao Paulo State Univ, Dept Morphol & Anim Physiol, Via Acesso Prof Paulo Donato Castelane, BR-14884900 Jaboticabal, SP, Brazil

Made available in DSpace on 2019-10-04T12:37:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01. Added 1 bitstream(s) on 2021-07-15T15:04:54Z : No. of bitstreams: 1 S1516-635X2019000100300.pdf: 163714 bytes, checksum: eb6392ce88cbd55548cc6e057bd545f6 (MD5) LOHMANN DO BRASIL(R) NOVA-TECH(R) Engineering This study aimed at evaluating beak-trimming methods and intensities on the performance, beak length and uniformity, and egg parameters of commercial layers. During the rearing period, birds were distributed in randomized blocks, with a 2x3 factorial design + two treatments, consisting of beak trimming with infrared (IR) radiation (moderate or severe); three IR intensities (low: 42 nm, intermediate: 46 nm, or high: 52 nm); and two control hot blade (HB) beak trimming treatments (moderate or severe) with eight treatments and five replicates of 21 chicks each, totaling 840 birds. During the growing phase (second experiment) the factorial design was modified to 2 x 3 x 2 + 4, comprising two IR beak-trimming intensities (moderate or severe); three IR radiation intensities (low, moderate and high), second beak trimming at 11 weeks of age or not, and four additional HB beak trimming treatments (moderate or severe) beak-trimming intensities, and second beak trimming at 11 weeks of age or not. There were 6 treatments with six replicates of seven birds each, totaling 672 laying hens, out of which 576 were selected for evaluation during the production period, with the same number of treatments and replicates of the previous phase. Weight gain (g), body weight (g), body weight uniformity (%), beak length (mm) and uniformity (%), absence of cannibalism (%), livability (%), egg production (%), feed intake (g), feed conversion per eggmass, eggmass, specific gravity, eggshell strength, Haugh unit, % yolk were evaluated. Beak trimming by infrared radiation may be employed in commercial laying hens. Univ Estado Mato Grosso, Dept Anim Sci, CP 181, BR-78250000 Pontes E Lacerda, MT, Brazil State Univ Sao Paulo, Dept Anim Prod, CP 560, BR-18618000 Botucatu, SP, Brazil Fed Univ West Para, Dept Anim Sci, Vera Paz S-N, BR-68035110 Santarem, PA, Brazil Fed Univ Vale Jequitinhonha & Mucuri, Dept Anim Sci, CP 34, BR-39100000 Diamantina, MG, Brazil Rural Fed Univ Pernambuco, Dept Anim Sci, Dom Manoel de Medeiros S-N, BR-52171900 Recife, PE, Brazil Fed Univ Grande Dourados, Dept Anim Sci, CP 533, BR-79804970 Diamantina, MG, Brazil Lohmann Brasil, BR-15020360 Sao Jose Do Rio Preto, SP, Brazil State Univ Sao Paulo, Dept Anim Prod, CP 560, BR-18618000 Botucatu, SP, Brazil

Made available in DSpace on 2018-11-29T08:13:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-01-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) When the environment on which the animals are raised is very diverse, selecting the best sires for different environments may require the use of models that account for genotype by environment interaction (G x E). The main objective of this study was to evaluate the existence of G x E for yearling weight (YW) in Nellore cattle using reaction norm models with only pedigree and pedigree combined with genomic relationships. Additionally, genomic regions associated with each environment gradient were identified. A total of 67,996 YW records were used in reaction norm models to calculate EBV and genomic EBV. The method of choice for genomic evaluations was single-step genomic BLUP (ssGBLUP). Traditional and genomic models were tested on the ability to predict future animal performance. Genetic parameters for YW were obtained with the average information restricted maximum likelihood method, with and without adding genomic information for 5,091 animals. Additive genetic variances explained by windows of 200 adjacent SNP were used to identify genomic regions associated with the environmental gradient. Estimated variance components for the intercept and the slope in traditional and genomic models were similar. In both models, the observed changes in heritabilities and genetic correlations for YW across environments indicate the occurrence of genotype by environment interactions. Both traditional and genomic models were capable of identifying the genotype by environment interaction; however, the inclusion of genomic information in reaction norm models improved the ability to predict animals' future performance by 7.9% on average. The proportion of genetic variance explained by the top SNP window was 0.77% for the regression intercept (BTA5) and 0.82% for the slope (BTA14). Single-step GBLUP seems to be a suitable model to predict genetic values for YW in different production environments. Sao Paulo State Univ, Dept Anim Sci, FCAV, BR-14884900 Jaboticabal, Brazil Univ Georgia, Dept Anim & Dairy Sci, Athens, GA 30602 USA Fed Inst Sci & Technol Goiano, Campus Rio Verde, BR-75901970 Rio Verde, Go, Brazil Fed Univ Grande Dourados UFGD, Coll Agr Sci, Dourados, MS, Brazil Sao Paulo State Univ, Dept Anim Sci, FCAV, BR-14884900 Jaboticabal, Brazil CAPES: PDSE 012708/2013-05 FAPESP: 2009/16118-5

Made available in DSpace on 2018-11-26T17:44:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-12-01 Foundation to Support to Development of Education, Science and Technology of Mato Grosso do Sul State (FUNDECT) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Successive applications of insecticides to control Plutella xylostella L. (Lepidoptera: Plutellidae) have resulted in the emergence of resistant populations of this insect. A novel control measure for this target insect could be the use of botanical insecticides derived from plant tissues. Hence, we experimentally tested aqueous extracts of Alibertia edulis (Rich.), Alibertia intermedia (Mart.), and Alibertia sessilis (Vell.) K. Schum. found in the Brazilian savannah in order to investigate their potential to disrupt the life cycle of P. xylostella. Aqueous extracts of the leaves of A. intermedia and A. sessilis negatively affected the development of P. xylostella in all stages of the life cycle, prolonging the larval stage and causing mortality in the larval or pupal stages. Treatments with A. intermedia and A. sessilis extracts caused the lowest fecundity and the number of hatched larvae. The harmful effects of these aqueous extracts on the life cycle of P. xylostella may be attributable to the flavonoids and other phenolic compounds present in A. intermedia and A. sessilis. These aqueous botanical extracts are low in toxicity when compared to non-aqueous pesticides, and may emerge as an effective approach for control of populations of P. xylostella. Fed Univ Grande Dourados, Fac Biol & Environm Sci, Lab Insect Plant Interact, Highway Dourados Itahum,Km 12, BR-79804970 Dourados, MS, Brazil Fed Univ Grande Dourados, Fac Biol & Environm Sci, Biol Control Lab, Highway Dourados Itahum,Km 12, BR-79804970 Dourados, MS, Brazil Univ Estadual Mato Grosso do Sul, Chem Lab, Highway Dourados Itahum,Km 12, BR-79804970 Dourados, MS, Brazil Fed Univ Grande Dourados, Fac Agr Sci, Lab Vegetables Prod, Highway Dourados Itahum,Km 12, BR-79804970 Dourados, MS, Brazil Sao Paulo State Univ, Fac Agron Sci, Dept Vegetal Prod, Lab Social Insects Pest,Phytosanitary Def, St Jose Barbosa de Barros, BR-18610307 Sao Paulo, Brazil Sao Paulo State Univ, Fac Agron Sci, Dept Vegetal Prod, Lab Social Insects Pest,Phytosanitary Def, St Jose Barbosa de Barros, BR-18610307 Sao Paulo, Brazil Foundation to Support to Development of Education, Science and Technology of Mato Grosso do Sul State (FUNDECT): 59/300.029/2015 CNPq: 304302/2015-5 FAPESP: 2008/00522-9

Made available in DSpace on 2018-11-26T17:54:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-09-01 Foundation for the Support and Development of Education, Science, and Technology (FUNDECT) of the state of Mato Grosso do Sul Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Biomonitoring is one of the tools used to assess the mutagenic potential of the atmosphere. In this study, the mutagenicity of Tradescantia pallida, a species of plant largely present in urban environments, was investigated. The objectives of this study was to estimate the mutagenic potential of vehicular flow through the TRAD-MCN bioassay in cities located at different altitudes in the southwest mesoregion of Mato Grosso do Sul, Brazil, to infer possible abiotic agents that may contribute to the effects of atmospheric pollutants, and finally to map the cities with greater risks to the health of the local population. To achieve these objectives, the Tradescantia-micronucleus test was performed on young buds of T. pallida collected between August 2015 and August 2016 in nine cities of Mato Grosso do Sul. These buds were exposed to traffic flows of various intensities. The data collected consisted of measurements of meteorological parameters and vehicular traffic counts for each city. The variables considered were: mean ambient temperature; micronuclei frequency; vehicular flow; altitude; relative humidity; pluviosity. The application of the Trad-MCN bioassay, with the consideration of environmental variables and altitudes, and the use of the Kernel interpolation technique, allowed us to map the areas with significant pollution risks to the population. The highest frequency of exposure to mutagens occurred in the cities with the highest vehicular traffic intensity. The average ambient temperature failed to show a linear association with the frequency of the micronuclei in the samples analyzed (r = 0.11(ns)). A positive correlation was observed between micronuclei frequency and vehicular flow, (r = 0.67; p

Made available in DSpace on 2018-11-26T15:44:15Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-04-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) The boll weevil, Anthonomus grandis grandis Boheman (Coleoptera: Curculionidae), is one of the most important pests of cotton production worldwide. The objective of this work was to develop a sequential sampling plan for the boll weevil. The studies were conducted in Maracaju, MS, Brazil, in two seasons with cotton cultivar FM 993.A 10,000-m(2) area of cotton was subdivided into 100 of 10- by 10-m plots, and five plants per plot were evaluated weekly, recording the number of squares with feeding + oviposition punctures of A. grandis in each plant. A sequential sampling plan by the maximum likelihood ratio test was developed, using a 10% threshold level of squares attacked. A 5% security level was adopted for the elaboration of the sequential sampling plan. The type I and type II error used was 0.05, recommended for studies with insects. The adjustment of the frequency distributions used were divided into two phases, so that the model that best fit to the data was the negative binomial distribution up to 85 DAE (Phase I), and from there the best fit was Poisson distribution (Phase II). The equations that define the decision-making for Phase I are S-0 = -5.1743 + 0.5730N and S-1 - 5.1743 + 0.5730N, and for the Phase II are S-0 = -4.2479 + 0.5771N and S-1 = 4.2479 + 0.5771N. The sequential sampling plan developed indicated the maximum number of sample units expected for decision-making is similar to 39 and 31 samples for Phases I and II, respectively. Fdn MS, CP 137, BR-79150000 Maracaju, MS, Brazil Sao Paulo State Univ FCAV UNESP, Via Acesso Prof Paulo Donato Castellane,S-N, BR-14884900 Jaboticabal, SP, Brazil Embrapa Agr Oeste, CP 449, BR-79804970 Dourados, MS, Brazil Fed Univ Grande Dourados FCBA UFGD, CP 533, BR-79804970 Dourados, MS, Brazil Sao Paulo State Univ FCAV UNESP, Via Acesso Prof Paulo Donato Castellane,S-N, BR-14884900 Jaboticabal, SP, Brazil

Made available in DSpace on 2019-10-06T08:12:05Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-09-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundação para o Desenvolvimento da UNESP (FUNDUNESP) NVIDIA Corporation Foundation to Support the Development of Teaching, Science and Technology of the state of Mato Grosso do Sul (FUNDECT) The annotation of large datasets is an issue whose challenge increases as the number of labeled samples available to train the classifier reduces in comparison to the amount of unlabeled data. In this context, semi-supervised learning methods aim at discovering and propagating labels to unsupervised samples, such that their correct labeling can improve the classification performance. Our proposal makes use of semi-supervised methodologies to classify an unlabeled training set that is used to train a Convolution Neural Network using different training strategies. The proposed approach is experimentally validated for soybean leaf and herbivorous pest identification using images captured by Unmanned Aerial Vehicles and can support specialists and farmers in the pest control management in soybean fields, especially when they have a limited amount of labeled samples. Fed Univ Grande Dourados, BR-79804970 Dourados, Brazil Univ Catolica Dom Bosco, BR-79117900 Campo Grande, Brazil Sao Paulo State Univ UNESP, BR-17033360 Bauru, Brazil Sao Paulo State Univ UNESP, BR-17033360 Bauru, Brazil CNPq: 427968/2018-6 CNPq: 307066/2017-7 FAPESP: 2013/07375-0 FAPESP: 2014/12236-1 FAPESP: 2015/25739-4 FAPESP: 2016/19403-6 FUNDUNESP: 2597.2017

Made available in DSpace on 2018-11-26T17:10:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-10-01 Skeletal abnormalities, bone deformities and fractures cause significant losses in broiler production during both rearing and processing. Silicon is an essential mineral for bone and connective tissue synthesis and for calcium absorption during the early stages of bone formation.Performance was not affected by the addition of silicon. However, broilers receiving silicon showed a significant increase of phosphorus, zinc, copper, manganese and ash in the tibia.In conclusion, broiler performance was not impaired by adding the tested silicon product to the drinking water. In addition, bone development improved, as demonstrated by higher mineral and ash content.Further studies are required to determine the optimal concentration of silicon, including heat stress simulations, to better understand the effects of silicon on bone development. Fed Univ Grande Dourados, Coll Agr Sci, Km 12,Postal Code 533, BR-79804970 Dourados, MS, Brazil Sao Paulo State Univ, Dept Morphol & Anim Physiol, Jaboticabal, Brazil Univ Fed Goias, Coll Agr Sci, Reg Jatai, Jatai, Brazil Sao Paulo State Univ, Dept Morphol & Anim Physiol, Jaboticabal, Brazil

Made available in DSpace on 2018-11-26T17:16:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-10-01. Added 1 bitstream(s) on 2021-07-15T15:01:35Z : No. of bitstreams: 1 S1516-635X2016000600041.pdf: 244221 bytes, checksum: 7af79ecb06a75851f7e834f2a94cff5b (MD5) Adequate environmental temperature control is essential for incubation efficiency. Layer breeder eggs (n=360) were weighed and distributed in a completely randomized experimental design with two treatments, consisting of two incubation temperatures (T1=37.5 degrees C, control; and T2=39.0 degrees C, hot), with two incubators per temperature, and 90 eggs per incubator, totaling 360 eggs. Hatchability, embryo mortality, and chick cloacal and body surface temperatures were not affected by incubation temperature. Eggs incubated at the hot temperature presented greater egg mass loss and higher eggshell conductance than those incubated at the control temperature. Layer chicks derived from eggs incubated at control temperature showed greater absolute weight, yolk-free egg mass, and heavier hearts than those from eggs submitted to heat stress during incubation. The control group presented lower base excess and ionized calcium blood levels. Incubating eggs at temperatures higher than those recommended compromises body and heart development of layer chicks and negatively affects blood ionized calcium availability, and therefore, bone mineralization during embryo development. Efficient temperature control during the incubation of fertile eggs is essential to obtain good quality layer chicks. Brazil Univ, BR-13690970 Descalvado, SP, Brazil Sao Paulo State Univ, Dept Morphol & Anim Physiol, Anim Sci, BR-14884900 Jaboticabal, SP, Brazil Fed Univ Grande Dourados, Postdoctoral Program Agribusiness, Coll Agr Sci, BR-79804970 Dourados, MS, Brazil Sao Paulo State Univ, Dept Morphol & Anim Physiol, BR-14884900 Jaboticabal, SP, Brazil Fed Univ Grande Dourados, Coll Agr Sci, BR-79804970 Dourados, MS, Brazil Sao Paulo State Univ, Dept Morphol & Anim Physiol, Anim Sci, BR-14884900 Jaboticabal, SP, Brazil Sao Paulo State Univ, Dept Morphol & Anim Physiol, BR-14884900 Jaboticabal, SP, Brazil

Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:26:26Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:36:41Z : No. of bitstreams: 1 2-s2.0-84865355009.pdf: 444618 bytes, checksum: 603fbef80b761dcf67d0bf1107c787c5 (MD5) Made available in DSpace on 2014-05-27T11:26:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-04-01 Broiler chicken production is widely dispersed across the globe, and one important issue for growers is the selection of adequate bedding material, as the availability and price of substrates varies among countries and regions within a same country. This study aimed at applying a multiple criteria analysis approach for the selection of the most appropriate bedding material for broiler production. Based on field research data and growers' experience, the most desirable characteristics of a litter material were chosen as the main criteria. The selected materials were wood shavings, rice husks, chopped Napier grass (Pennisetum pupureum), 50% sugar cane bagasse (Saccharum L.) plus 50% wood shavings, 50% sugar cane bagasse (Saccharum L.) plus 50% rice husks, and pure sugar cane bagasse (Saccharum L.). The analytical hierarchy process (AHP) was applied for selecting the most suitable bedding material. Validation was performed using data from previous studies carried out in central-western Brazil on the effects of different types of bedding material on broiler carcass quality. Considering the selected criteria, several bedding materials were tested and ranked, and the results showed that wood-shavings litter was the best option (weight = 0.28), followed by rice husks (weight = 0.24). All other tested alternatives presented lower scores and were, therefore, not considered for use. The AHP approach was found to be an efficient tool to select the most appropriate litter material under specific scenarios. College of Animal Science Federal University of Grande Dourados - UFGD, Dourados, MS Experimental Unit of Tupã State University of São Paulo - UNESP, Tupã, SP Experimental Unit of Tupã State University of São Paulo - UNESP, Tupã, SP

Made available in DSpace on 2018-11-26T15:29:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Peptidases are important because they play a central role in pharmaceutical, food, environmental, and other industrial processes. A serine peptidase from Aspergillus terreus was isolated after two chromatography steps that showed a yield of 15.5%. Its molecular mass was determined to be 43 kD, by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). This peptidase was active between pH 5.0 to 8.0 and had maximum activity at pH 7.0, at 45 degrees C. When exposited with 1 M of urea, the enzyme maintained 100% activity and used azocasein as substrate. The N-terminal (first 15 residues) showed 33% identity with the serine peptidase of Aspergillus clavatus ES1. The kinetics assays showed that subsite S-2 did not bind polar basic amino acids (His and Arg) nonpolar acidic amino acids (Asp and Glu). The subsite S-1 showed higher catalytic efficiency than the S-2 and S-3 subsites. Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Dept Pharmaceut Sci, S-N Cafe, BR-14040903 Ribeirao Preto, Brazil Univ Estadual Paulista, Inst Biosci Letters & Exact Sci, Sao Jose Do Rio Preto, Brazil Fed Univ Grande Dourados, Fac Biol & Environm Sci, Dourados, Brazil Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Dept Chem & Phys, BR-14040903 Ribeirao Preto, Brazil Univ Ctr Educ Fdn Barretos, Fac Pharm, Barretos, Brazil Univ Fed Sao Paulo, Paulista Sch Med, Dept Biophys, Sao Paulo, Brazil Univ Estadual Paulista, Inst Biosci Letters & Exact Sci, Sao Jose Do Rio Preto, Brazil FAPESP: 2011/06986-0 FAPESP: 2012/24703-8 CNPq: 308078/2012-8

Made available in DSpace on 2018-11-26T15:28:07Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-12-01 Fundect Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Composite films based on Poly(vinylidene fluoride) (PVDF) polymer filled with Nickel oxide (NiO) nanosheets were prepared via the solution casting method. The effect of NiO filler introduction on the structure and morphology of the composite films were analyzed by X-ray diffraction, Fourier transformed infrared spectroscopy and Scanning Electron Microscopy. The results showed that the crystallinity, beta-phase content and morphology are directly related to the NiO filler concentration into the polymer matrix owing to a heterogeneous nucleation. Electrical measurements revealed dielectric constants up to 45 at 1 kHz and an increasing in conductivity more than two orders of magnitude, with a percolation threshold of about 4.0% v/v of NiO filler content. (C) 2015 Elsevier Ltd and Techna Group S.r.l. All rights reserved. Fed Univ Grande Dourados, GOA, Appl Opt Grp, Dourados, MS, Brazil Sao Paulo State Univ, Inst Chem, LIEC Dept Chem Techonol, Interdisciplinary Lab Electrochem & Ceram, Araraquara, SP, Brazil Univ Grande Dourados, Lab Organ Catalysis & Biocatalysis, Dourados, MS, Brazil Univ Estadual Maringa, Mat Chem & Sensors Lab LMSen, Maringa, Parana, Brazil Sao Paulo State Univ, Inst Chem, LIEC Dept Chem Techonol, Interdisciplinary Lab Electrochem & Ceram, Araraquara, SP, Brazil Fundect: 15743.291.3846.25112009 FAPESP: 13/14647-6 FAPESP: 2012/11979-5 FAPESP: 2013/07296-2 CNPq: 310820/2011-1 CNPq: 2008232/2014-1 CNPq: 483683/2010-8

Fundacao de Apoio ao Desenvolvimento do Ensino Ciencia e Tecnologia do Mato Grosso do Sul (FUNDECT) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Background and Purpose: Leaves of Jacaranda decurrens are used in traditional Brazilian medicine to treat metabolic diseases related to increased reactive oxygen species. the present study evaluated the antioxidant and cytotoxic potential of hydroethanolic extract from the leaves of Jacaranda decurrens subsp. symmetrifoliolata.Experimental Approach: Phenolic compounds, flavonoids and saponins were evaluated in an ethanol: water (80: 20, v/v) extract from the leaves of Jacaranda decurrens subsp. symmetrifoliolata (E-Jds). the antioxidant activity of E-Jds was investigated by assessing the following: 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity; protection against 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced hemolysis of erythrocytes; in vitro and in vivo malondialdehyde dosage; and the ability to activate antioxidant enzymes. K562 leukemia cells were used for the cytotoxic evaluation of E-Jds and for the assessment of the cell death profile through flow cytometry.Key Results: Phenolic and flavonoid compounds were quantified as 14.38% and 2.15%, respectively, of E-Jds. These phenolic and flavonoid compounds proved to be able to scavenge DPPH free radicals with an IC50 of 9.3 +/- 3.3 mu g/mL, to protect up to 50% of erythrocytes against AAPH-induced hemolysis and to reduce in vitro and in vivo malondialdehyde levels up to 84% and 22%, respectively. E-Jds also increased glutathione peroxidase enzyme activity, with a concomitant decrease in superoxide dismutase and catalase activity, and exhibited dose-dependent cytotoxic activity on K562 erythroleukemia cells with cell death occurring via both late apoptosis and necrosis.Conclusions: E-Jds exhibits in vitro and in vivo antioxidant potential, which may be the mechanism mediating the metabolic activities reported in folk medicine. Furthermore, the cytotoxic activity identified in this study contributes with the knowledge of antiproliferative activities that have been described in the literature for the genus Jacaranda. Fed Univ Grande Dourados, Dourados, MS, Brazil Univ Estadual Mato Grosso do Sul, Dourados, MS, Brazil Univ Fed Alagoas, Maceio, Alagoas, Brazil Universidade Federal de São Paulo, São Paulo, Brazil Universidade Federal de São Paulo, EPM, São Paulo, Brazil Web of Science

Made available in DSpace on 2018-11-26T17:06:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-09-01. Added 1 bitstream(s) on 2021-07-15T15:01:30Z : No. of bitstreams: 1 S0103-90162016000500406.pdf: 150918 bytes, checksum: d1c8fe2b77b88c580f36c63da500ce93 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The effect of additives used in the feed of broilers on anaerobic bio-digestion of poultry litter was evaluated. Four diets were used: NC: negative control; DFM: NC + 500 ppm direct-fed microbials (DFM) containing Bacillus subtilis and Bacillus licheniformis; ENZ: diet formulated with an enzyme blend (20 ppm phytase, 200 ppm protease and 200 ppm xylanase); DFM+E: ENZ + DFM. Substrates for the anaerobic bio-digestion were prepared with litter from each treatment, containing 4 % total solids (TS). These were used in 16 continuous bio-digesters with a 2 kg d(-1) load, to determine the production and potential biogas production and composition during an 85-day period. Influent and effluent samples were collected for the amounts of TS and volatile solids (VS), fiber fraction (neutral detergent fiber [NDF], acid detergent fiber [ADF] and lignin), nutrients (N, P and K), and total and thermotolerant coliforms to be determined. For all treatments a reduction in the following effluents was observed as follows: TS (49, 48, 48 and 50 %) VS (70, 54, 55 and 62 %) NDF (91, 90, 95 and 96 %) ADF (89, 88, 93 and 94 %) and lignin (80, 76, 89 and 88 %). The efficiency of the treatment for coliforms in bio-digesters was higher than 90 % in the 85-day period in all treatment groups. There was a reduction in biogas and methane production when DFM (5500 and 4000 mL) and DFM + E (5800 and 4100 mL) were used, compared to treatments NC (6300 mL and 4400) and ENZ (6400 and 4500 mL). The potential production of reduced TS and VS was higher in ENZ (1: 00 and 1.74 106 mL kg(-1)) when compared to NC (0.88 and 1: 02 106 mL kg(-1)), DFM (0.80 and 1:40 106 mL kg(-1)) and DFM + E (0.88 1: 25 and 106 mL kg(-1)). The additives did not affect the percentage of methane production, and all treatments showed values higher than 70 %. Adding enzymes to the diet of broilers influences the litter characteristics and, as a consequence, increases biogas production. The addition of DFM and DFM + E to broiler diets reduced biogas and methane production. Sao Paulo State Univ, FMVZ, Dept Nutr & Anim Prod, Av Duque de Caxias 225, BR-13635900 Pirassununga, SP, Brazil Fed Univ Grande Dourados, Sch Agr Sci, Rod Dourados Itahum,Km 12,Unidade II,CP 364, BR-79804970 Dourados, MS, Brazil DSM Nutr Prod, Av Eng Billings,1729 Predio 31, BR-05321010 Sao Paulo, SP, Brazil Sao Paulo State Univ, FCAV, Dept Anim Morphol & Physiol, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, Brazil Fed Univ Grande Dourados, Sch Business Adm Acconting & Econ Sci, Dourados, MS, Brazil Sao Paulo State Univ, FMVZ, Dept Nutr & Anim Prod, Av Duque de Caxias 225, BR-13635900 Pirassununga, SP, Brazil Sao Paulo State Univ, FCAV, Dept Anim Morphol & Physiol, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, Brazil FAPESP: 2010/05263-1 FAPESP: 2010/11329-5