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21 results on '"Falke, Floris"'

7. Full integration of photonic nanoimmunosensors in portable platforms for on-line monitoring of ocean pollutants

Author

European Commission, Ministerio de Economía y Competitividad (España), Generalitat de Catalunya, Fernández Gavela, Adrián, Herranz, Sonia, Chocarro Ruiz, Blanca, Falke, Floris, Schreuder, Erik, Leeuwis, Henk, Heideman, René G., Lechuga, Laura M., European Commission, Ministerio de Economía y Competitividad (España), Generalitat de Catalunya, Fernández Gavela, Adrián, Herranz, Sonia, Chocarro Ruiz, Blanca, Falke, Floris, Schreuder, Erik, Leeuwis, Henk, Heideman, René G., and Lechuga, Laura M.

Abstract

We have developed a photonic nano-immunosensor platform for the on-site analysis of harmful organic ocean pollutants, intended to be allocated in stand-alone buoys. The main aim is bringing the monitoring tools directly to the contaminated place, resulting in cost and time savings as compared to the standard analytical techniques. As sensor we have employed an integrated asymmetric Mach-Zehnder interferometer (aMZI) of micro/nano dimensions, based on silicon photonic technology. In order to obtain a multiplexed system, a four-channel microfluidic cell has been designed, manufactured and incorporated in the miniaturized sensor. Additionally, a microfluidic delivery module enabling automatic sample analysis has been designed, evaluated and assembled. Moreover, we have implemented the optical interconnections of the sensor chip by fiber optics, as well the electronics and the required software and data processing. Pollutant detection is based on a competitive immunoassay using bioreceptors previously biofunctionalized on the aMZI sensor arms and incubation with a specific antibody. As proof of concept, two types of pollutants have been analysed: the biocide Irgarol 1051, and the antibiotic Tetracycline. Results show limits of detection in the range of few ng/mL, accomplished the European legislation.

Published
2019

14. Biosensors based on Si3N4asymmetric Mach-Zehnder interferometers

Author

Chalyan, Tatevik, additional, Pasquardini, Laura, additional, Falke, Floris, additional, Zanetti, Manuela, additional, Guider, Romain, additional, Gandolfi, Davide, additional, Schreuder, Eric, additional, Pederzolli, Cecilia, additional, Heideman, René G., additional, and Pavesi, Lorenzo, additional

Published
2016
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21. AFM1 Detection in Milk by Fab' Functionalized Si 3 N 4 Asymmetric Mach-Zehnder Interferometric Biosensors.

Author

Chalyan T, Potrich C, Schreuder E, Falke F, Pasquardini L, Pederzolli C, Heideman R, and Pavesi L

Subjects
Aflatoxin M1 chemistry, Aflatoxin M1 immunology, Animals, Enzyme-Linked Immunosorbent Assay, Immunoglobulin Fab Fragments chemistry, Immunoglobulin Fab Fragments immunology, Interferometry, Light, Silicon Compounds chemistry, Aflatoxin M1 analysis, Biosensing Techniques, Food Contamination analysis, Milk chemistry
Abstract

Aflatoxins (AF) are naturally occurring mycotoxins, produced by many species of Aspergillus. Among aflatoxins, Aflatoxin M1 (AFM1) is one of the most frequent and dangerous for human health. The acceptable maximum level of AFM1 in milk according to EU regulation is 50 ppt, equivalent to 152 pM, and 25 ppt, equivalent to 76 pM, for adults and infants, respectively. Here, we study a photonic biosensor based on Si 3 N 4 asymmetric Mach-Zehnder Interferometers (aMZI) functionalized with Fab' for AFM1 detection in milk samples (eluates). The minimum concentration of AFM1 detected by our aMZI sensors is 48 pM (16.8 pg/mL) in purified and concentrated milk samples. Moreover, the real-time detection of the ligand-analyte binding enables the study of the kinetics of the reaction. We measured the kinetic rate constants of the Fab'-AFM1 interaction.

Published
2019
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