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AFM1 Detection in Milk by Fab' Functionalized Si 3 N 4 Asymmetric Mach-Zehnder Interferometric Biosensors.
- Source :
-
Toxins [Toxins (Basel)] 2019 Jul 14; Vol. 11 (7). Date of Electronic Publication: 2019 Jul 14. - Publication Year :
- 2019
-
Abstract
- Aflatoxins (AF) are naturally occurring mycotoxins, produced by many species of Aspergillus. Among aflatoxins, Aflatoxin M1 (AFM1) is one of the most frequent and dangerous for human health. The acceptable maximum level of AFM1 in milk according to EU regulation is 50 ppt, equivalent to 152 pM, and 25 ppt, equivalent to 76 pM, for adults and infants, respectively. Here, we study a photonic biosensor based on Si 3 N 4 asymmetric Mach-Zehnder Interferometers (aMZI) functionalized with Fab' for AFM1 detection in milk samples (eluates). The minimum concentration of AFM1 detected by our aMZI sensors is 48 pM (16.8 pg/mL) in purified and concentrated milk samples. Moreover, the real-time detection of the ligand-analyte binding enables the study of the kinetics of the reaction. We measured the kinetic rate constants of the Fab'-AFM1 interaction.
- Subjects :
- Aflatoxin M1 chemistry
Aflatoxin M1 immunology
Animals
Enzyme-Linked Immunosorbent Assay
Immunoglobulin Fab Fragments chemistry
Immunoglobulin Fab Fragments immunology
Interferometry
Light
Silicon Compounds chemistry
Aflatoxin M1 analysis
Biosensing Techniques
Food Contamination analysis
Milk chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 2072-6651
- Volume :
- 11
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Toxins
- Publication Type :
- Academic Journal
- Accession number :
- 31337103
- Full Text :
- https://doi.org/10.3390/toxins11070409