1. Acquired enamel pellicle protects gastroesophageal reflux disease patients against erosive tooth wear
- Author
-
Tatiana MARTINI, Daniela RIOS, Aline DIONIZIO, Luiza de Paula Silva CASSIANO, Cíntia Maria de Souza e SILVA, Even Akemi TAIRA, Talita Mendes Oliveira VENTURA, João Victor Frazão CÂMARA, Tamara Teodoro Araujo, Ana Carolina MAGALHÃES, Thiago Saads CARVALHO, Tommy BAUMANN, Adrian LUSSI, Ricardo Brandt de OLIVEIRA, Regina Guenka PALMA-DIBB, and Marília Afonso Rabelo BUZALAF
- Subjects
Tooth Erosion ,Dental Pellicle ,Proteomics ,Dentistry ,RK1-715 - Abstract
Abstract The objective of this study was to compare the protein profile of the acquired enamel pellicle (AEP) formed in vivo in patients with or without gastroesophageal reflux disease (GERD), and with or without erosive tooth wear (ETW). Twenty-four volunteers were divided into 3 groups: 1) GERD and ETW; 2) GERD without ETW; and 3) control (without GERD). The AEP formed 120 min after prophylaxis was collected from the lingual/palatal surfaces. The samples were subjected to mass spectrometry (nLC-ESI-MS/MS) and label-free quantification by Protein Lynx Global Service software. A total of 213 proteins were identified, or 119, 92 and 106 from each group, respectively. Group 2 showed a high number of phosphorylated and calcium-binding proteins. Twenty-three proteins were found in all the groups, including 14-3-3 protein zeta/delta and 1-phosphatidylinositol. Several intracellular proteins that join saliva after the exfoliation of oral mucosa cells might have the potential to bind hydroxyapatite, or participate in forming supramolecular aggregates that bind to precursor proteins in the AEP. Proteins might play a central role in protecting the dental surface against acid dissolution.
- Published
- 2023
- Full Text
- View/download PDF