22 results on '"Eui-Ryong Chung"'
Search Results
2. Novel SNPs in the bovine ADIPOQ and PPARGC1A genes are associated with carcass traits in Hanwoo (Korean cattle)
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Eui-Ryong Chung and Sungchul Shin
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Candidate gene ,Meat ,Genotype ,Population ,Single-nucleotide polymorphism ,Quantitative trait locus ,Biology ,ADIPOQ Gene ,Polymorphism, Single Nucleotide ,Quantitative Trait, Heritable ,Gene Frequency ,Gene Order ,Genetics ,Animals ,education ,Molecular Biology ,Alleles ,Genetic Association Studies ,education.field_of_study ,Chromosome Mapping ,Sequence Analysis, DNA ,General Medicine ,Minor allele frequency ,Haplotypes ,Hanwoo ,Cattle ,Adiponectin ,PPARGC1A ,Transcription Factors - Abstract
Adiponectin (ADIPOQ) modulates several biological processes including energy homeostasis, glucose and lipid metabolism. The bovine ADIPOQ gene was located near the QTL affecting marbling, ribeye muscle area and fat thickness on BTA1. The gene encoding peroxisome proliferator-activated receptor-γ coactivator-1α (PPARGC1A) was located within the QTL region of the traits on BTA6. Moreover, its protein product has various biological functions such as cellular energy homeostasis, including adaptive thermogenesis, adipogenesis and gluconeogenesis. Therefore, the ADIPOQ and PPARGC1A genes are a positional and functional candidate gene for carcass traits in beef cattle. The objectives of this study were to identify polymorphisms in the bovine ADIPOQ and PPARGC1A genes, to evaluate their associations with carcass traits in Hanwoo (Korean cattle) population. We identified nine SNPs in the ADIPOQ gene. Two SNPs (DQ156119: g.1436T > C and DQ156119: g.1454A > G) in the promoter region were recognized as new SNPs identified in Hanwoo. Association analysis indicated that the g.1454A > G SNP genotype was significantly associated with effects on LMA (P = 0.004) and BF (P = 0.021). The ADIPOQ haplotype was also found to have significant effect on the LMA. In the PPARGC1A gene, we identified 11 SNPs in the two unexplored regions (intron 3 and 5). Among them, seven SNPs were located in intron 3 and four SNPs were located in intron 5. Of these 11 putative novel SNPs, two SNPs (AY839822: g.292C > T and AY839823: g.1064C > T) with minor allele frequency (MAF) > 0.20 were examined for associations with carcass traits. The association analysis revealed that both SNPs in PPARGC1A gene were significantly associated with LMA (P < 0.05). These findings suggest that the SNPs of bovine ADIPOQ and PPARGC1A genes may be a useful molecular marker for selection of carcass traits in Hanwoo.
- Published
- 2013
3. Acceleration of Cell Proliferation and Gene Expression in Human Chondrosarcoma Cells Stimulated by Strong Pulse Magnetic Field
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Do Guwn Hwang, Eui Ryong Chung, and Sung Chul Shin
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Chemistry ,Cell growth ,Cartilage ,Stimulation ,Condensed Matter Physics ,medicine.disease ,Chondrocyte ,Electronic, Optical and Magnetic Materials ,Cell biology ,Extracellular matrix ,medicine.anatomical_structure ,Gene expression ,medicine ,Electrical and Electronic Engineering ,Chondrosarcoma ,Gene - Abstract
For the treatment of osteoarthritis, pulsed electromagnetic field stimulus has been suggested as a useful therapeutic method in rehabilitative medicine. Most studies have been performed under low-frequency and lowenergy to find out biological properties for stimulating chondrocyte with pulsed magnetic field. In this study, the effect of strong pulse magnetic field on the human chondrosarcoma cells (SW-1353) has been investigated by means of cell counting, morphologies, and gene expression of cartilage extracellular matrix genes. The SW-1353 cells were exposed under the field intensities of 270, 100, 55, 36, and 26 mTesla during 6 hours a day in 5 consecutive days. The pulse magnetic field with an LRC oscillating signal has the pulse width of 0.126 msec and stimulation period of 1 sec. For the 270 and 100 mTesla stimulation, the cell proliferation significantly increased in 21-24% as compared with the non-stimulated cells. Gene expression of cartilage extracellular matrix genes (ACAN, COMP and COL2A1) was assayed by quantitative real time-PCR method. The ACAN gene expression showed a significant brightness, which means the increase on gene expression, compared with the non-stimulated cells. Our results suggest that the strong pulse magnetic field stimulation can be utilized to accelerate cell proliferation and gene expression on human chondrosarcoma cells.
- Published
- 2013
4. Effect of Single Nucleotide Polymorphism of Endothelial Differentiation G-Protein Coupled Receptor 1 (EDG1) Gene on Marbling Score in Hanwoo
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Eui-Ryong Chung and Sung Chul Shin
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Genetics ,Candidate gene ,Marbled meat ,Hanwoo ,SNP ,Animal Science and Zoology ,Single-nucleotide polymorphism ,Intramuscular fat ,Biology ,Beef cattle ,Quantitative trait locus ,Food Science - Abstract
Marbling (intramuscular fat) is the most economically important meat quality trait in Hanwoo (Korean cattle). The endot-helial differentiation G-protein coupled receptor 1 (EDG1) gene, involved in blood vessel formation, is located within thegenomic region of a quantitative trait locus (QTL) for marbling on bovine chromosome 3. Thus, the EDG1 gene can beconsidered as a positional and functional candidate gene for meat quality in beef cattle. This study aimed to identify singlenucleotide polymorphisms (SNPs) in the EDG1 gene and to evaluate their associations with carcass traits in Hanwoo pop-ulation. We have sequenced a fragment of 5'-UTR of the EDG1 gene and identified one SNP. Genotyping of the g.166A>GSNP marker was carried out using PCR-RFLP analysis in 309 Hanwoo steers in order to evaluate their association withcarcass traits. The g.166A>G SNP marker showed a significant effect on the marbling score. Animals with the GG genotypehad higher marbling score compared with AA and AG genotypes (p
- Published
- 2012
5. Single nucleotide polymorphisms in exomes of high- and low-marbled meats in Korean cattle
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Sungchul Shin, Kung Ahn, Changpyo Hong, Eui-Ryong Chung, and Sanghoon Song
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0301 basic medicine ,Genetics ,Meat ,Marbled meat ,0402 animal and dairy science ,Single-nucleotide polymorphism ,04 agricultural and veterinary sciences ,General Medicine ,Sequence Analysis, DNA ,Biology ,040201 dairy & animal science ,Polymorphism, Single Nucleotide ,03 medical and health sciences ,030104 developmental biology ,Adipose Tissue ,Republic of Korea ,Animals ,Animal Science and Zoology ,Cattle ,Exome ,Exome sequencing - Published
- 2016
6. Genetic variants of the FABP4 gene are associated with marbling scores and meat quality grades in Hanwoo (Korean cattle)
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Sungchul Shin, Jae Pil Heo, and Eui Ryong Chung
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Heterozygote ,Meat ,Genotype ,Marbled meat ,Molecular Sequence Data ,Single-nucleotide polymorphism ,Biology ,Quantitative trait locus ,Fatty Acid-Binding Proteins ,Polymerase Chain Reaction ,Polymorphism, Single Nucleotide ,Loss of heterozygosity ,Quantitative Trait, Heritable ,Gene Frequency ,Republic of Korea ,Genetics ,Animals ,SNP ,Least-Squares Analysis ,Molecular Biology ,Allele frequency ,Genetic Association Studies ,Electrophoresis, Agar Gel ,Base Sequence ,General Medicine ,Hanwoo ,Cattle ,Polymorphism, Restriction Fragment Length - Abstract
This study was aimed to search new genetic variants in the bovine FABP4 gene as molecular markers for meat quality and carcass traits. PCR-RFLP analysis revealed that three SNPs located at nucleotide positions g.2834C>G, g.3533T>A, and g.3691G>A were identified based on a GenBank accession number (NC_007312.4). Sequence analysis revealed that SNPs were located in intron 1 (g.2834C>G) and 2 (g.3533T>A), and an exon 3 (g.3691G>A), showing allele frequencies as 0.592, 0.579, and 0.789, respectively. Genetic variabilities of heterozygosity (He) and polymorphic information contents (PIC) were estimated for g.2834C>G (0.608 and 0.531), g.3533T>A (0.615 and 0.539), and g.3691G>A (0.498 and 0.401) loci, respectively. A SNP located in the exon 3 of FABP4 was characterized and associated with desirable increases of MS (marbling scores) and MG (meat quality grades) in Hanwoo. The statistical analysis revealed that additive effects by GG genotypes in g.3691G>A SNP were significantly greater than AA genotypes in MS and MG traits. These findings suggest that the FABP4g.3691G>A SNP will be a useful candidate locus to maximize economic benefits for cattle populations.
- Published
- 2011
7. Association between SNP Marker of Uncoupling Protein 3 Gene and Meat Yield and Marbling Score Traits in Korean Cattle
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Eui-Ryong Chung, Sung Chul Shin, and Jae-Pil Heo
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Genetics ,Candidate gene ,Marbled meat ,Genotype ,SNP ,Animal Science and Zoology ,Single-nucleotide polymorphism ,White adipose tissue ,Intramuscular fat ,Biology ,Food Science ,UCP3 - Abstract
It is well established that uncoupling protein 3 (UCP3) is expressed largely in skeletal muscle, white adipose tissue and brown adipose tissue and has been suggested to play important roles in regulating energy expenditure, body weight, thermoregulation as well as fatty acid metabolism and obesity. Therefore, the UCP3 gene was selected as a candidate gene for carcass and meat quality traits in Korean cattle. The objective of this study was to identify single nucleotide polymorphisms (SNPs) in the UCP3 gene and to evaluate the association of UCP3 SNP markers with carcass and meat quality traits in Korean cattle. The five exons in the UCP3 gene were sequenced, and ten SNPs were identified. The PCR-SSCP method was then developed to genotype the individuals examined. The g.3076A>G genotype was significantly associated with marbling score (MS) of Korean cattle. Animals with the AA genotype had a higher MS than those with the AG and GG genotypes. No significant associations of the SNP g.3076A>G were observed for any traits. In conclusion, although SNP g.3076A>G, which showed an association with MS, does not cause amino acid changes, this SNP may be used as a DNA marker to select animals that have higher intramuscular fat content.
- Published
- 2011
8. Effect of Single Nucleotide Polymorphisms of Acetyl-CoA Carboxylase α (ACACA) Gene on Carcass Traits in Hanwoo (Korean Cattle)
- Author
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Eui-Ryong Chung, Jae-Pil Heo, and Sung Chul Shin
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Genetics ,ACACA ,Candidate gene ,Haploview ,Haplotype ,Hanwoo ,Acetyl-CoA carboxylase ,food and beverages ,Animal Science and Zoology ,Single-nucleotide polymorphism ,Biology ,Beef cattle ,Food Science - Abstract
Meat production and quality traits in beef cattle are largely affected by genetic factors. Acetyl-Coenzyme A carboxylase-α (ACACA) plays a key role in the regulation and metabolism of fatty acid biosynthesis in mammalian animals. The gene encoding ACACA enzyme was chosen as a candidate gene for carcass and meat traits. In this study, we investigated effects of single nucleotide polymorphisms (SNPs) in the ACACA gene on beef carcass and meat traits in Hanwoo (Korean cattle) populations. We have sequenced a fragment of intron I region of the Hanwoo ACACA gene and identified two SNPs. Genotyping of the two SNP markers (g.2344T>C and g.2447C>A) was carried out using PCR-SSCP analysis in 309 Hanwoo steers to evaluate their association with carcass and meat production traits. The g.2344C SNP marker showed a significant increasing effect on LW (p = 0.009) and CW (p = 0.017). Animals with the CC genotype had higher CW and LW compared with TT and TC genotypes (p C and g.2447C>A in the LD analysis, four major haplotype classes were formed with two SNP information within the ACACA gene. We constructed haplotypes using the HaploView software package program and analyzed association between haplotypes and carcass traits. The haplotype of ACACA gene significantly affected the LW (p = 0.027), CW (p = 0.041) and MC (p = 0.036). The effect of h1 haplotype on LW and CW was larger than that of h3 haplotype. Animals with the h1 haplotype also had greater MC than did animals with h2 haplotype. Consequently, the ACACA gene could be useful as a DNA marker for meat production traits such as carcass yield and meat contents in Hanwoo. (Key
- Published
- 2011
9. Identification of Korean Native Pork Using Breed-Specific DNA Marker of KIT Gene
- Author
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Eui-Ryong Chung and Ku-Young Chung
- Subjects
Genetics ,Coat ,Kit gene ,Intron ,Amplicon ,Biology ,Molecular biology ,Breed ,Korean Native ,chemistry.chemical_compound ,chemistry ,Genetic marker ,Animal Science and Zoology ,DNA ,Food Science - Abstract
Accurate methods for the identification of closely related species or breeds in raw and processed meats must be developed in order to protect both consumers and producers from mislabeling and fraud. This paper describes the development of DNA markers for the discrimination and improvement of Korean native pig (KNP) meat. The KIT gene is related to pig coat color and is often used as a candidate marker. A 538 bp fragment comprising intron 19 of the pig KIT gene was amplified by PCR using specific primers, after which the PCR amplicons of a number of meat samples from KNP and three major improved breeds (Landrace, Duroc and Yorkshire) were sequenced in order to find a nucleotide region suitable for PCR-RFLP analysis. Sequence data showed the presence of two nucleotide substitutions, g.276G>A and g.295A>C, between KNP and the improved pig breeds. Digestion of KIT amplicons with AccII enzyme generated characteristic PCR-RFLP profiles that allowed discrimination between meats from KNP and improved pig. KNP showed three visible DNA bands of 264/249, 199, and 75 bp, whereas DNA bands of 249, 199, and 90 bp were detected in the three improved pig breeds. Therefore, the 75 bp DNA fragment was specific only to KNP, whereas the 90 bp DNA fragment was specific to the improved breeds. The breed-specific DNA markers reported here that target the KIT gene could be useful for the identification of KNP meat from improved pig meats, thus contributing to the prevention of falsified breed labeling.
- Published
- 2010
10. Effects of SNP Markers of the Apolipoprotein E (APOE) Gene on Meat Quantity and Quality Traits in Korean Cattle
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Eui-Ryong Chung, Ku-Young Chung, Sung Chul Shin, and Ki-Hyun Shin
- Subjects
Genetics ,Apolipoprotein E ,Candidate gene ,Exon ,Genotype ,SNP ,lipids (amino acids, peptides, and proteins) ,Animal Science and Zoology ,Single-nucleotide polymorphism ,Biology ,Allele ,Beef cattle ,Food Science - Abstract
Apolipoprotein E (APOE) is a plasma lipoprotein in mammals and plays an important role in the transport and metabolism of lipids such as phospholipids and triglycerides. Therefore, the APOE gene could be a candidate gene controlling lipid metabolism in beef cattle. This study was performed to identify single nucleotide polymorphisms (SNP) in the APOE gene and to investigate the effects of SNP genotype on the carcass traits such as meat quantity and quality in Korean cattle. For PCR amplification, pooled DNA made from unrelated 60 individuals was prepared and primer pairs were designed based on the cDNA sequence of exon 4 region of the bovine APOE gene. A SNP was identified at position 2034 (T/C substitution) of the exon 4 region in the APOE gene. PCR-RFLP procedure with restriction enzyme ACC I was developed for determining the SNP genotype for each of a total of 309 animals with pedigree information and performance records through the national progeny testing program. The frequencies of the genotypes TT, TC and CC were 10.9, 46.9 and 42.2%. Gene frequencies were 0.344 for T allele and 0.656 for C allele. The g.2034T>C SNP genotype showed a significant effect (p C SNP genotype of the APOE gene may be useful as a DNA marker for meat quantity index and dressing percentage in Korean cattle.
- Published
- 2009
11. SNP Discovery in the Leptin Promoter Gene and Association with Meat Quality and Carcass Traits in Korean Cattle
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K. H. Shin, Ku-Young Chung, Eui-Ryong Chung, and Sung Chul Shin
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Genetics ,Candidate gene ,Leptin ,Genotype ,SNP ,Animal Science and Zoology ,Promoter ,Single-nucleotide polymorphism ,Beef cattle ,Biology ,Gene ,Food Science - Abstract
Leptin, the hormone product of the obese gene, is secreted predominately from white adipose tissue and regulates feed intake, energy metabolism and body composition. It has been considered a candidate gene for performance, carcass and meat quality traits in beef cattle. The objective of this study was to identify SNPs in the promoter region of the leptin gene and to evaluate the possible association of the SNP genotypes with carcass and meat quality traits in Korean cattle. We identified a total of 25 SNPs in the promoter region (1,208-3,049 bp upstream from the transcription start site) of the leptin gene, eleven (g.1508C>G, g.1540G>A, g.1545G>A, g.1551C>T, g.1746T>G, g.1798ins(G), g.1932del(T), g.1933del(T), g.1934del(T), g.1993C>T and g.2033C>T) of which have not been reported previously. Their sequences were deposited in GenBank database with accession number DQ202319. Genotyping of the SNPs located at positions g.2418C>G and g.2423G>A within the promoter region was performed by direct sequencing and PCR-SSCP method to investigate the effects of SNP genotypes on carcass and meat quality traits in Korean cattle. The SNP and SSCP genotypes from the two mutations of the leptin promoter were shown to be associated with the BF trait. The average BF value of animals with heterozygous SNP genotype was significantly greater than that of animals with the homozygous SNP genotypes for the g.2418C>G and g.2423G>A SNPs (p
- Published
- 2008
12. Association Analysis between Insulin-like Growth Factor Binding Protein 3 (IGFBP3) Polymorphisms and Carcass Traits in Cattle
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Hye Won Lee, Byung Lae Park, Eui Ryong Chung, Il-Cheong Cheong, Lyoung Hyo Kim, Hyun Sub Cheong, Hyoung Doo Shin, Duhak Yoon, Eun Mi Kim, and Sohg Namgoong
- Subjects
Genetics ,Korean Native ,Candidate gene ,Polymorphism (computer science) ,Marbled meat ,Haplotype ,Hanwoo ,IGFBP3 ,Animal Science and Zoology ,Biology ,Beef cattle ,Food Science - Abstract
The insulin-like growth factor binding protein 3 (IGFBP3) has been investigated as a candidate gene for growth promoting effects in beef cattle and a modulator of IGF bioactivity. Previously, we have reported twenty two sequence variants discovered in Korean native cattle (Hanwoo). In this study, we examined the association between gene-specific polymorphisms of IGFBP3 and cold carcass weight (CW) and marbling score (MS) among Korean native cattle. Among twenty two polymorphisms, four common polymorphic sites (-854G>C, -100G>A, +421G>T and +3863C>A) were genotyped in our beef cattle (n = 437). Statistical analysis revealed that one common polymorphism in the promoter region (-854G>C) showed putative associations with MS (p = 0.03). IGFBP3 variation/haplotype information analyzed in this study will provide valuable information into strategies for the production of a commercial line of beef cattle.
- Published
- 2008
13. Identification of Meat Species Using Species-Specific PCR-RFLP Fingerprint of Mitochondrial 12S rRNA Gene
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Sung Chul Shin, Eui-Ryong Chung, Ki-Hyun Shin, Ku-Young Chung, and Jong Keun Park
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Genetics ,chemistry.chemical_classification ,Mitochondrial DNA ,Amplicon ,Biology ,Molecular biology ,law.invention ,Restriction enzyme ,chemistry ,DNA profiling ,law ,Animal Science and Zoology ,Nucleotide ,Restriction fragment length polymorphism ,Gene ,Polymerase chain reaction ,Food Science - Abstract
In order to develop a sensitive and reliable method for the species-specific molecular markers, PCR-RFLP assay of the mitochondrial DNA(mt DNA) 12S rRNA gene was exploited for the identification of the origin of animal meat species including cattle, pig, sheep, goat, horse, deer, chicken, duck and turkey. A specific primer pairs were designed, based on the nucleotide sequences of mt 12S rRNA gene, for the amplification of the highly conserved region in the gene of the animal species using PCR-RFLP technique. mt DNA was isolated from meat samples followed by DNA amplification using PCR with the specific primers. PCR amplification produced an approximately 455 bp fragment in each of these animal meats. To distinguish pleat species, the PCR amplicons were digested with restriction endonucleases Tsp5091 and MboI, respectively, which generates distinct RFLP profiles. The DNA profiles digested with Tsp5091 allowed the clear discrimination in the mammalian meat species and the DNA profiles digested with MboI in poultry meat species. Therefore, the PCR-RFLP profiles of mt 12S rRNA gene could be very useful to identify the origin of the raw materials in the raw meats as well as the processed meat products.
- Published
- 2007
14. Leptin Polymorphisms Associated with Carcass Traits of Meat in Korean Cattle
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Duhak Yoon, Hyun Sub Cheong, Hyoung Doo Shin, Lyoung Hyo Kim, Sung-Jong Oh, Byung Lae Park, Han Ju Lee, Eui Ryong Chung, and Il-Cheong Cheong
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Genetics ,Candidate gene ,Animal science ,Polymorphism (computer science) ,Leptin ,Marbled meat ,Genotype ,Trait ,Animal Science and Zoology ,Single-nucleotide polymorphism ,Beef cattle ,Biology ,Food Science - Abstract
Leptin has been investigated as a candidate gene for fat characteristics in beef cattle. Previously, we have reported 57 sequence variants discovered in Korean cattle (Bos Taurus coreanae). In this study, we examined the association between polymorphisms of leptin and carcass traits (cold carcass weight (CWT) and marbling score (Marb)) in Korean cattle. Among 57 polymorphisms, 11 common polymorphic sites were genotyped in our beef cattle (n = 437). Statistical analysis revealed that one single nucleotide polymorphism in coding exon (c.+411T>C (A137A)) showed a significant association with the yield trait, CWT. The C-bearing genotypes (CC or CT) of c.+411T>C (A137A) showed the higher CWT (p = 0.006). c.+150C>G (S50S) also showed a significant association with the quality trait, Marb (p = 0.01). Our findings suggest that polymorphisms in leptin might be one of the important genetic factors that influence carcass yield and quality in beef cattle, especially in CWT and Marb.
- Published
- 2006
15. Highly Polymorphic Bovine Leptin Gene
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I. C. Cheong, Byung-Lae Park, Eui Ryong Chung, Han-Chul Lee, Duhak Yoon, Y. H. Choi, B. H. Cho, Hyoung Doo Shin, and Hyun Sub Cheong
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Genetics ,Linkage disequilibrium ,Exon ,Genetic linkage ,Haplotype ,Animal Science and Zoology ,Single-nucleotide polymorphism ,Biology ,Tag SNP ,Gene ,Allele frequency ,Food Science - Abstract
The leptin, an anti-obesity protein, is a hormone protein expressed and secreted mainly from adipocyte tissue, and involved in regulation of body weight, food intake and energy metabolism. In an effort to discover polymorphism(s) in genes whose variant(s) might be implicated in phenotypic traits of growth, we have sequenced exons and their boundaries of leptin gene including 1,000 bp upstream of promoter region with twenty-four unrelated Korean cattle. Fifty-seven sequence variants were identified: fourteen in 5' flanking region, twenty-seven in introns, eight in exons, and eight in 3' flanking region. By pair-wise linkage analysis among polymorphisms, ten sets of SNPs were in absolute linkage disequilibrium (LD) (|D'| = 1 and r 2 = 1). Among variants identified, thirty-six SNPs were newly identified, and twenty-one SNPs, which were reported in other breeds, were also confirmed in Korean cattle. The allele frequencies of variants were quite different among breeds. The information from SNPs of bovine leptin gene could be useful for further genetic studies of this gene. (Asian-Aust. J. Anim. Sci. 2005. Vol 18, No. 11 : 1548-1551)
- Published
- 2005
16. Identification of Novel SNPs in Bovine Insulin-like Growth Factor Binding Protein-3 (IGFBP3) Gene
- Author
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Duhak Yoon, Jun Yeun Kim, Eui Ryong Chung, J.G. Choi, K.J. Na, Sung-Jong Oh, Lyoung-Ho Kim, Byung-Lae Park, Han-Chul Lee, C.Y. Cho, Il-Cheong Cheong, Hyoung Doo Shin, and B.C. Sang
- Subjects
Genetics ,Linkage disequilibrium ,Hanwoo ,Haplotype ,Intron ,IGFBP3 ,Animal Science and Zoology ,Single-nucleotide polymorphism ,Promoter ,Biology ,Gene ,Molecular biology ,Food Science - Abstract
The insulin-like growth factors (IGFs), their receptors, and their binding proteins play key roles in regulating cell proliferation and apoptosis. Insulin-like growth factor binding protein-3 (IGFBP3, OMIM #146732) is one of the proteins that bind to the IGFs. IGFBP3 is a modulator of IGF bioactivity, and direct growth inhibitor in the extravascular tissue compartment. We identified twenty-two novel single nucleotide polymorphisms (SNPs) in IGFBP3 gene in Korean cattle (Hanwoo, Bos taurus coreanae) by direct sequencing of full gene including -1,500 bp promoter region. Among the identified SNPs, five common SNPs were screened in 650 Korean cattle; one SNP in promoter (IGFBP3 G-854C), one in 5'UTR region (IGFBP3 G-100A), two in intron 1 (IGFBP3 G+421T, IGFBP3 T+1636A), and one in intron 2 (IGFBP3 C+3863A). The frequencies of each SNP were 0.357 (IGFBP3 G−854C), 0.472 (IGFBP3 G-100A), 0.418 (IGFBP3 G+421T), 0.363 (IGFBP3 T+1636A) and 0.226 (IGFBP3 C+3863A), respectively. Haplotypes and their frequencies were estimated by EM algorithm. Six haplotypes were constructed with five SNPs and linkage disequilibrium coefficients (|D'|) between SNP pairs were also calculated. The information on SNPs and haplotypes in IGFBP3 gene could be useful for genetic studies of this gene. (Asian-Aust. J. Anim. Sci. 2005. Vol 18, No. 1 : 3-7)
- Published
- 2005
17. Effects of genetic variants for the bovine calpain gene on meat tenderness
- Author
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Eui-Ryong Chung, Sungchul Shin, and Hoyoung Chung
- Subjects
Genetic Markers ,Meat ,Genotype ,Biology ,Polymorphism, Single Nucleotide ,Linkage Disequilibrium ,Meat tenderness ,Gene Frequency ,Genetic variation ,Gene Order ,Genetics ,Animals ,Allele ,Molecular Biology ,Alleles ,Genetic Association Studies ,Calpain ,Genetic Variation ,Single-strand conformation polymorphism ,General Medicine ,Genetic marker ,Hanwoo ,Cattle ,Restriction fragment length polymorphism - Abstract
The objective of this study was to determine whether the genetic variants of CAPN1 developed in several cattle populations can be applied for Hanwoo, regarding genetic effects on meat traits. The traits were examined for 286 purebred Hanwoo steers with genotypes classified by restriction fragment length polymorphism (RFLP) and single strand conformation polymorphism (SSCP) analysis. The nucleotide positions of primers and previously identified genetic variants were based on sequences of the calpain 1 (CAPN1) gene with GenBank accession numbers (AF252504, AF248054, and AY639597). The analysis of genetic distribution estimated levels of minor allele frequencies ranged from 0.165 to 0.392, showing no significant departures from Hardy-Weinberg Equilibrium for all markers. Overall averages of heterozygosites (He) and polymorphic information contents (PICs) for all markers were calculated to 0.503 and 0.429, respectively, and the g.4558G>A marker showed the lowest He (0.425) and PIC (0.367). Animals from 29 months of age were slaughtered to measure Warner-Bratzler shear force (WBSF), cooking loss, water-holding capacity, pH, fat, and moisture. All the CAPN1 markers explained variations of WBSF, showing significant additive effects except g.5709G>A. A significant marginal mean difference in genotypes of g.6545C>T (P=0.046) was found in moisture with additive effects. From the result it may be possible to use three calpain markers (g.4558G>A, g.4685C>T, and g.6545C>T) classified by RFLP and SSCP analysis in marker assisted selection programs to improve WBSF as meat tenderness in Hanwoo.
- Published
- 2013
18. A previously unreportedDraI polymorphism within the regulatory region of the bovinegrowth hormonegene and its association with growth traits in Korean Hanwoo cattle
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J. H. Joh, Eui-Ryong Chung, C. S. Lee, O. H. Kim, Y. W. Seo, N. K. Kim, and G. H. Kim
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Genetics ,Korea ,Polymorphism, Genetic ,Base Sequence ,Molecular Sequence Data ,Sequence Analysis, DNA ,General Medicine ,Regulatory Sequences, Nucleic Acid ,Biology ,Regulatory region ,Species Specificity ,Polymorphism (computer science) ,Growth Hormone ,Hanwoo ,Linear Models ,Animals ,Body Weights and Measures ,Cattle ,Animal Science and Zoology ,Bovine somatotropin ,Gene ,Polymorphism, Restriction Fragment Length ,DNA Primers - Published
- 2004
19. Genetic association of phosphodiesterase 1B (PDE1B) with carcass traits in Korean cattle
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Jae-Pil Heo, Eui-Ryong Chung, Chaeyoung Lee, Jung-Sou Yeo, and Sungchul Shin
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Genetic Markers ,Candidate gene ,Meat ,Single-nucleotide polymorphism ,Quantitative trait locus ,Beef cattle ,Biology ,Polymerase Chain Reaction ,Polymorphism, Single Nucleotide ,Exon ,Quantitative Trait, Heritable ,Gene Frequency ,Genotype ,Republic of Korea ,Genetics ,Animals ,Molecular Biology ,Genetic Association Studies ,Genetic association ,Nucleotides ,food and beverages ,General Medicine ,Cyclic Nucleotide Phosphodiesterases, Type 1 ,Haplotypes ,Cattle ,Restriction fragment length polymorphism ,Polymorphism, Restriction Fragment Length - Abstract
Quantitative trait loci for fat deposition and carcass traits have been identified in the vicinity of the gene encoding phosphodiesterase 1B (PDE1B) on bovine chromosome 5. Therefore, the PDE1B gene can be considered as a positional and functional candidate gene for carcass traits in beef cattle. This study aimed to identify single nucleotide polymorphisms (SNPs) in the PDE1B gene and to evaluate their associations with carcass traits in Korean cattle. Eight SNPs, g.440TG, g.17122AG, g.17507AC, g.17575AG, g.17607TC, g.17609CA, g.17692CT, and g.17707CG, were identified in the region ranged from exon 1 to intron 6. Five of them were used for association analysis because of their availability of restriction fragment length polymorphisms. As a result, g.17122AG in intron 3 was significantly associated with backfat thickness (BFT), and g.17507AC in exon 5 was associated with longissimus dorsi muscle area (LMA, P 0.05). Animals with the AG genotype of g.17122 had thicker BFT than those with the AA genotype. Animals with the AA or AC genotype of g.17507AC had larger LMA than those with the CC genotype. We suggested the PDE1B gene as a candidate gene for carcass traits of beef cattle. Fine mapping would be required for application to marker-assisted selection.
- Published
- 2011
20. Fatty acid composition of beef is associated with exonic nucleotide variants of the gene encoding FASN
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Eui-Ryong Chung, Yoonseok Lee, Dong-Yep Oh, Chaeyoung Lee, Younyoung Kim, Jung-Sou Yeo, and Boomi La
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medicine.medical_specialty ,Linkage disequilibrium ,Meat ,Single-nucleotide polymorphism ,Polymorphism, Single Nucleotide ,Linkage Disequilibrium ,Gene Frequency ,Internal medicine ,Genotype ,Republic of Korea ,Genetics ,medicine ,Animals ,Molecular Biology ,Unsaturated fatty acid ,Genetic Association Studies ,Genetic association ,Adiposity ,chemistry.chemical_classification ,biology ,Nucleotides ,Muscles ,Haplotype ,Fatty Acids ,Fatty acid ,General Medicine ,Exons ,Fatty acid synthase ,Endocrinology ,Phenotype ,chemistry ,Haplotypes ,biology.protein ,Cattle ,Female ,Fatty Acid Synthases - Abstract
Genetic associations of fatty acid composition with exonic single nucleotide polymorphisms (SNPs) in the gene encoding fatty acid synthase (FASN) were examined using 513 Korean cattle. All five individual SNPs of g.12870 T>C, g.13126 T>C, g.15532 C>A, g.16907 T>C and g.17924 G>A were associated with a variety of fatty acid compositions and further with marbling score (P
- Published
- 2011
21. Association of bovine fatty acid composition with novel missense nucleotide polymorphism in thethyroid hormone-responsive(THRSP) gene
- Author
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Eui-Ryong Chung, Jung-Sou Yeo, Chaeyoung Lee, Dong-Yep Oh, Yongseok Lee, Sungchul Shin, and Boomi La
- Subjects
Male ,Hormone Responsive ,Genetics ,Fatty Acids ,Thyroid ,Mutation, Missense ,Nuclear Proteins ,Single-nucleotide polymorphism ,Exons ,Sequence Analysis, DNA ,General Medicine ,Biology ,Polymorphism, Single Nucleotide ,medicine.anatomical_structure ,Republic of Korea ,medicine ,Animals ,Missense mutation ,Cattle ,Animal Science and Zoology ,Fatty acid composition ,THRSP Gene ,Genetic Association Studies ,Transcription Factors - Published
- 2012
22. Novel SNP in the coding region of the FTO gene is associated with marbling score in Hanwoo (Korean cattle)
- Author
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Eui-Ryong Chung
- Subjects
Genetics ,education.field_of_study ,Candidate gene ,Ecology ,Research ,Veterinary (miscellaneous) ,Marbled meat ,Population ,SNP ,Single-nucleotide polymorphism ,Marbling ,Beef cattle ,Biology ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,FTO gene ,Hanwoo ,Animal Science and Zoology ,Meat quality ,FTO ,education ,Food Science - Abstract
The fat mass and obesity associated (FTO) gene plays an important role in the regulation of energy homeostasis, fat deposition and obesity. For this reason, the FTO gene is a physiological and functional candidate gene for carcass and meat quality traits in beef cattle. The objectives of this study were to identify SNPs in the exonic regions of FTO gene and to evaluate the association of these SNPs with carcass traits in Hanwoo (Korean cattle). In this study, we newly identified two exonic SNPs in Hanwoo population. The g.125550A > T SNP was located in exon 3 and the g.175675C > T SNP was located in exon 6. Genotyping of the two SNP markers was carried out using PCR-RFLP analysis in Hanwoo steers to evaluate their association with carcass traits. As a result, g.125550A > T SNP genotype was significantly associated with effects on marbling score. Animals with the AA and TT homozygous genotypes had a significantly higher marbling score (p T SNP. Our results suggest that the exonic SNP g.125550A > T in the FTO gene may be used as a DNA marker for the selection of Hanwoo with higher marbling.
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