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Novel SNPs in the bovine ADIPOQ and PPARGC1A genes are associated with carcass traits in Hanwoo (Korean cattle)
- Source :
- Molecular Biology Reports. 40:4651-4660
- Publication Year :
- 2013
- Publisher :
- Springer Science and Business Media LLC, 2013.
-
Abstract
- Adiponectin (ADIPOQ) modulates several biological processes including energy homeostasis, glucose and lipid metabolism. The bovine ADIPOQ gene was located near the QTL affecting marbling, ribeye muscle area and fat thickness on BTA1. The gene encoding peroxisome proliferator-activated receptor-γ coactivator-1α (PPARGC1A) was located within the QTL region of the traits on BTA6. Moreover, its protein product has various biological functions such as cellular energy homeostasis, including adaptive thermogenesis, adipogenesis and gluconeogenesis. Therefore, the ADIPOQ and PPARGC1A genes are a positional and functional candidate gene for carcass traits in beef cattle. The objectives of this study were to identify polymorphisms in the bovine ADIPOQ and PPARGC1A genes, to evaluate their associations with carcass traits in Hanwoo (Korean cattle) population. We identified nine SNPs in the ADIPOQ gene. Two SNPs (DQ156119: g.1436T > C and DQ156119: g.1454A > G) in the promoter region were recognized as new SNPs identified in Hanwoo. Association analysis indicated that the g.1454A > G SNP genotype was significantly associated with effects on LMA (P = 0.004) and BF (P = 0.021). The ADIPOQ haplotype was also found to have significant effect on the LMA. In the PPARGC1A gene, we identified 11 SNPs in the two unexplored regions (intron 3 and 5). Among them, seven SNPs were located in intron 3 and four SNPs were located in intron 5. Of these 11 putative novel SNPs, two SNPs (AY839822: g.292C > T and AY839823: g.1064C > T) with minor allele frequency (MAF) > 0.20 were examined for associations with carcass traits. The association analysis revealed that both SNPs in PPARGC1A gene were significantly associated with LMA (P < 0.05). These findings suggest that the SNPs of bovine ADIPOQ and PPARGC1A genes may be a useful molecular marker for selection of carcass traits in Hanwoo.
- Subjects :
- Candidate gene
Meat
Genotype
Population
Single-nucleotide polymorphism
Quantitative trait locus
Biology
ADIPOQ Gene
Polymorphism, Single Nucleotide
Quantitative Trait, Heritable
Gene Frequency
Gene Order
Genetics
Animals
education
Molecular Biology
Alleles
Genetic Association Studies
education.field_of_study
Chromosome Mapping
Sequence Analysis, DNA
General Medicine
Minor allele frequency
Haplotypes
Hanwoo
Cattle
Adiponectin
PPARGC1A
Transcription Factors
Subjects
Details
- ISSN :
- 15734978 and 03014851
- Volume :
- 40
- Database :
- OpenAIRE
- Journal :
- Molecular Biology Reports
- Accession number :
- edsair.doi.dedup.....90c6d7838b8228c202c08a34e79fac91