5 results on '"Espuche B"'
Search Results
2. Nanogels: Smart tools to enlarge the therapeutic window of gene therapy.
- Author
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Espuche B, Moya SE, and Calderón M
- Subjects
- Humans, Nanogels, Drug Delivery Systems, Genetic Therapy, COVID-19 Vaccines, Nucleic Acids
- Abstract
Gene therapy can potentially treat a great number of diseases, from cancer to rare genetic disorders. Very recently, the development and emergency approval of nucleic acid-based COVID-19 vaccines confirmed its strength and versatility. However, gene therapy encounters limitations due to the lack of suitable carriers to vectorize therapeutic genetic material inside target cells. Nanogels are highly hydrated nano-size crosslinked polymeric networks that have been used in many biomedical applications, from drug delivery to tissue engineering and diagnostics. Due to their easy production, tunability, and swelling properties they have called the attention as promising vectors for gene delivery. In this review, nanogels are discussed as vectors for nucleic acid delivery aiming to enlarge gene therapy's therapeutic window. Recent works highlighting the optimization of inherent transfection efficiency and biocompatibility are reviewed here. The importance of the monomer choice, along with the internal structure, surface decoration, and responsive features are outlined for the different transfection modalities. The possible sources of toxicological endpoints in nanogels are analyzed, and the strategies to limit them are compared. Finally, perspectives are discussed to identify the remining challenges for the nanogels before their translation to the market as transfection agents., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
3. Self-assembly of NrTP6 cell-penetrating lipo-peptide with variable number of lipid chains: Impact of phosphate ions on lipid association.
- Author
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Phungula A, Waddad AY, Fernandez Leyes MD, Di Gianvincenzo P, Espuche B, Zuffi S, Moya SE, Albericio F, and de la Torre BG
- Subjects
- Protein Structure, Secondary, Water chemistry, Circular Dichroism, Lipopeptides chemistry, Micelles
- Abstract
Hypothesis: Lipopeptides synthesized from the Nucleolar Targeting Peptide (NrTP6) with one, two or four dodecanoic fatty acid (FA) chains, display large head to tail volumes, which together with the number of lipid chains per molecule, impacts their self-assembly behavior. In phosphate buffer (PB), peptide to peptide interactions are triggered by the presence of phosphate ions that act as ionic crosslinkers, affecting the organization of the lipid assemblies., Experimental: The NrTP6 lipopeptides were synthesized by the solid phase peptide synthesis technique. The critical micellar concentration (CMC) of the lipopeptides was determined in water and PB by pyrene fluorescence. The size and morphology of lipopeptide assemblies were characterized by dynamic light scattering (DLS) and transmission electron microscopy (TEM). Circular dichroism (CD) was used to study the secondary structures of the lipopeptide assemblies., Results: For NrTP6 lipopeptides with two and four lipid chains, CMCs in water are larger than in PB. TEM images of the lipopeptide assemblies show different morphologies including fibers, rods, and spheres depending on the number of lipid chains, concentration and whether they are assembled in water or PB. CD spectroscopy shows that the peptide conformation, either random or beta, correlates with the morphology of the assemblies., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Inc. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
4. Assembly and recognition mechanisms of glycosylated PEGylated polyallylamine phosphate nanoparticles: A fluorescence correlation spectroscopy and small angle X-ray scattering study.
- Author
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Perez Schmidt P, Luedtke T, Moretti P, Di Gianvincenzo P, Fernandez Leyes M, Espuche B, Amenitsch H, Wang G, Ritacco H, Polito L, Ortore MG, and Moya SE
- Subjects
- Concanavalin A, Lactose, Mannose, Scattering, Small Angle, X-Rays, Polyethylene Glycols chemistry, X-Ray Diffraction, Polyamines, Lectins chemistry, Polymers, Spectrum Analysis, Phosphates, Nanoparticles chemistry
- Abstract
Hypothesis: Modification of polyallylamine hydrochloride (PAH) with heterobifunctional low molecular weight polyethylene glycol (PEG) (600 and 1395 Da), and subsequent attachment of mannose, glucose, or lactose sugars to PEG, can lead to formation of polyamine phosphate nanoparticles (PANs) with lectin binding affinity and narrow size distribution., Experiments: Size, polydispersity, and internal structure of glycosylated PEGylated PANs were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS) and small angle X-ray scattering (SAXS). Fluorescence correlation spectroscopy (FCS) was used to study the association of labelled glycol-PEGylated PANs. The number of polymer chains forming the nanoparticles was determined from the changes in amplitude of the cross-correlation function of the polymers after formation of the nanoparticles. SAXS and fluorescence cross-correlation spectroscopy were used to investigate the interaction of PANs with lectins: concanavalin A with mannose modified PANs, and jacalin with lactose modified ones., Findings: Glyco-PEGylated PANs are highly monodispersed, with diameters of a few tens of nanometers and low charge, and a structure corresponding to spheres with Gaussian chains. FCS shows that the PANs are single chain nanoparticles or formed by two polymer chains. Concanavalin A and jacalin show specific interactions for the glyco-PEGylated PANs with higher affinity than bovine serum albumin., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Inc.)
- Published
- 2023
- Full Text
- View/download PDF
5. Facile MOF Support Improvement in Synergy with Light Acceleration for Efficient Nanoalloy-Catalyzed H 2 Production from Formic Acid.
- Author
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Liu Y, Fu F, Salmon L, Espuche B, Moya S, Berlande M, Pozzo JL, Hamon JR, and Astruc D
- Abstract
Hydrogen (H
2 ) generation and storage are actively investigated to provide a green source of energy, and formic acid (HCOOH), a major product obtained from the biomass, is regarded as a productive source of H2 . Therefore, improvements in heterogeneous catalysts are called for. Here, a novel type of catalyst support is proposed involving simple addition of the mixture of metal ion precursors to core-shell ZIF-8@ZIF-67, followed by reduction with NaBH4 , with performances surpassing those obtained using nanocatalysts in ZIF-8 or ZIF-67. The nanocatalysts Pdx Ag were optimized with ZIF-8@Pd2 Ag1 @ZIF-67 under visible-light illumination for selective HCOOH dehydrogenation involving a turnover frequency value of 430 h-1 under light irradiation at 353 K. These results also reveal the crucial roles of the Pd sites electronically promoted in the presence of visible light by the Ag plasmon resonance and the advantageous core-shell MOF structure. In order to examine the potential of extending this catalyst improvement principle to other catalytic reactions, 4-nitrophenol reduction, a benchmarking model of catalytic reaction, was tested, and the results also confirmed the superiority of the performance of ZIF-8@Pd2 Ag1 @ZIF-67 over Pd2 Ag1 @ZIF-8 and Pd2 Ag1 @ZIF-67, confirming the interest in the novel catalyst design.- Published
- 2023
- Full Text
- View/download PDF
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