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2. Genetic, Antigenic and Serologic Characterization of Human Immunodeficiency Virus Type 1 from Indonesia

Author

Anthony Rl, Hayes Cg, Francine E. McCutchan, Hupudio H, R R Graham, Kevin R. Porter, Corwin Al, Thomas C. VanCott, Ertono S, Mascola, Widodo S, Ewing D, Donald S. Burke, and Wignall Fs

Subjects
Male, Genotype, HIV Antigens, Sequence analysis, Molecular Sequence Data, Immunology, HIV Envelope Protein gp120, Biology, Polymerase Chain Reaction, Neutralization, Virus, Serology, Neutralization Tests, Risk Factors, Virology, HIV Seropositivity, parasitic diseases, Humans, Immunology and Allergy, Amino Acid Sequence, Typing, Serotyping, Clade, Phylogeny, Infectivity, Binding Sites, Immune Sera, virus diseases, Peptide Fragments, Military Personnel, Indonesia, CD4 Antigens, DNA, Viral, HIV-1, Female
Abstract

To examine the genetic and antigenic characteristics of HIV-1 in Indonesia, samples from 19 HIV-positive volunteers were studied. By a combination of PCR typing and DNA sequence analysis, 12 of the 19 volunteers were determined to be infected with HIV-1 clade B and seven with clade E. Six of the seven Indonesian clade E isolates were from volunteers associated with the Indonesian Military during a peacekeeping mission in Cambodia. Infectivity reduction neutralization assays showed that the Indonesian E viruses were effectively neutralized by Thailand clade E HIV-1 antisera but not by U.S. clade B antisera. The Indonesian clade B virus tested was neutralized by U.S. clade B antisera and not by the Thailand E antisera. Using a previously described serologic typing ELISA based on clade B and E V3 peptides, genetic clade was accurately determined in eight of eight sera tested. This is the first report of the genetic and antigenic analysis of HIV-1 isolates from Indonesia. The data indicate that at least two genetic and antigenic HIV-1 clades (clade E and B) circulate in Indonesia.

Published
1997

3. Genetic, antigenic and serologic characterization of human immunodeficiency virus type 1 from Indonesia

Author

Porter, KR, Mascola, JR, Hupudio, H, Ewing, D, VanCott, TC, Anthony, RL, Corwin, AL, Widodo, S, Ertono, S, McCutchan, FE, Burke, DS, Hayes, CG, Wignall, FS, Graham, RR, Porter, KR, Mascola, JR, Hupudio, H, Ewing, D, VanCott, TC, Anthony, RL, Corwin, AL, Widodo, S, Ertono, S, McCutchan, FE, Burke, DS, Hayes, CG, Wignall, FS, and Graham, RR

Abstract

To examine the genetic and antigenic characteristics of HIV-1 in Indonesia, samples from 19 HIV-positive volunteers were studied. By a combination of PCR typing and DNA sequence analysis, 12 of the 19 volunteers were determined to be infected with HIV-1 clade B and seven with clade E. Six of the seven Indonesian clade E isolates were from volunteers associated with the Indonesian Military during a peacekeeping mission in Cambodia. Infectivity reduction neutralization assays showed that the Indonesian E viruses were effectively neutralized by Thailand clade E HIV-1 antisera but not by U.S. clade B antisera. The Indonesian clade B virus tested was neutralized by U.S. clade B antisera and not by the Thailand E antisera. Using a previously described serologic typing ELISA based on clade B and E V3 peptides, genetic clade was accurately determined in eight of eight sera tested. This is the first report of the genetic and antigenic analysis of HIV-1 isolates from Indonesia. The data indicate that at least two genetic and antigenic HIV-1 clades (clade E and B) circulate in Indonesia.

Published
1997

4. HIV and peacekeeping operations in Cambodia

Author

Ertono S, Hudoyo H, Soeprapto W, Kevin R. Porter, Mascola J, Gunawan S, and Corwin Al

Subjects
Diarrhea, United Nations, Human immunodeficiency virus (HIV), HIV Infections, medicine.disease_cause, Environmental protection, Environmental health, HIV Seropositivity, Prevalence, Humans, Mass Screening, Medicine, Mass screening, business.industry, Follow up studies, Sequence Analysis, DNA, General Medicine, medicine.disease, Hiv seropositivity, Malaria, Military personnel, Military Personnel, Population Surveillance, HIV-1, Cambodia, business, Follow-Up Studies, Peacekeeping
Published
1995

5. Genetic, antigenic and serologic characterization of human immunodeficiency virus type 1 from Indonesia.

Author

Porter KR, Mascola JR, Hupudio H, Ewing D, VanCott TC, Anthony RL, Corwin AL, Widodo S, Ertono S, McCutchan FE, Burke DS, Hayes CG, Wignall FS, and Graham RR

Subjects
Amino Acid Sequence, Binding Sites, CD4 Antigens metabolism, DNA, Viral analysis, DNA, Viral chemistry, Female, Genotype, HIV Antigens immunology, HIV Envelope Protein gp120 chemistry, HIV Envelope Protein gp120 genetics, HIV Envelope Protein gp120 immunology, HIV Seropositivity epidemiology, HIV-1 genetics, HIV-1 immunology, Humans, Immune Sera immunology, Indonesia epidemiology, Male, Military Personnel, Molecular Sequence Data, Neutralization Tests, Peptide Fragments chemistry, Peptide Fragments genetics, Peptide Fragments immunology, Phylogeny, Polymerase Chain Reaction, Risk Factors, Serotyping, HIV Seropositivity virology, HIV-1 classification
Abstract

To examine the genetic and antigenic characteristics of HIV-1 in Indonesia, samples from 19 HIV-positive volunteers were studied. By a combination of PCR typing and DNA sequence analysis, 12 of the 19 volunteers were determined to be infected with HIV-1 clade B and seven with clade E. Six of the seven Indonesian clade E isolates were from volunteers associated with the Indonesian Military during a peacekeeping mission in Cambodia. Infectivity reduction neutralization assays showed that the Indonesian E viruses were effectively neutralized by Thailand clade E HIV-1 antisera but not by U.S. clade B antisera. The Indonesian clade B virus tested was neutralized by U.S. clade B antisera and not by the Thailand E antisera. Using a previously described serologic typing ELISA based on clade B and E V3 peptides, genetic clade was accurately determined in eight of eight sera tested. This is the first report of the genetic and antigenic analysis of HIV-1 isolates from Indonesia. The data indicate that at least two genetic and antigenic HIV-1 clades (clade E and B) circulate in Indonesia.

Published
1997
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