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Genetic, Antigenic and Serologic Characterization of Human Immunodeficiency Virus Type 1 from Indonesia
- Source :
- Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology. 14:1-6
- Publication Year :
- 1997
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 1997.
-
Abstract
- To examine the genetic and antigenic characteristics of HIV-1 in Indonesia, samples from 19 HIV-positive volunteers were studied. By a combination of PCR typing and DNA sequence analysis, 12 of the 19 volunteers were determined to be infected with HIV-1 clade B and seven with clade E. Six of the seven Indonesian clade E isolates were from volunteers associated with the Indonesian Military during a peacekeeping mission in Cambodia. Infectivity reduction neutralization assays showed that the Indonesian E viruses were effectively neutralized by Thailand clade E HIV-1 antisera but not by U.S. clade B antisera. The Indonesian clade B virus tested was neutralized by U.S. clade B antisera and not by the Thailand E antisera. Using a previously described serologic typing ELISA based on clade B and E V3 peptides, genetic clade was accurately determined in eight of eight sera tested. This is the first report of the genetic and antigenic analysis of HIV-1 isolates from Indonesia. The data indicate that at least two genetic and antigenic HIV-1 clades (clade E and B) circulate in Indonesia.
- Subjects :
- Male
Genotype
HIV Antigens
Sequence analysis
Molecular Sequence Data
Immunology
HIV Envelope Protein gp120
Biology
Polymerase Chain Reaction
Neutralization
Virus
Serology
Neutralization Tests
Risk Factors
Virology
HIV Seropositivity
parasitic diseases
Humans
Immunology and Allergy
Amino Acid Sequence
Typing
Serotyping
Clade
Phylogeny
Infectivity
Binding Sites
Immune Sera
virus diseases
Peptide Fragments
Military Personnel
Indonesia
CD4 Antigens
DNA, Viral
HIV-1
Female
Subjects
Details
- ISSN :
- 10779450
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology
- Accession number :
- edsair.doi.dedup.....c535f3c82c00d5a7205a124b5c30dd2e