1. Heightened expression of HLA-DQB1 and HLA-DQB2 in pre-implantation biopsies predicts poor late kidney graft function
- Author
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Helio Tedesco-Silva, Maria Gerbase-DeLima, Larissa A. Salzedas, Erika F. Campos, Claudia Rosso Felipe, Tuíla B. Mourão, Karina L. Mine, Jose O. Medina-Pestana, and Bruna L. Aguiar
- Subjects
Adult ,Graft Rejection ,Male ,Risk ,0301 basic medicine ,Oncology ,medicine.medical_specialty ,Adolescent ,Biopsy ,Immunology ,Human leukocyte antigen ,030230 surgery ,Kidney ,Graft function ,Young Adult ,03 medical and health sciences ,Postoperative Complications ,0302 clinical medicine ,Predictive Value of Tests ,HLA-DQ Antigens ,Internal medicine ,medicine ,HLA-DQ beta-Chains ,Humans ,Immunology and Allergy ,Gene ,Kidney transplantation ,HLA-DQB1 ,Microarray analysis techniques ,business.industry ,General Medicine ,Middle Aged ,Prognosis ,medicine.disease ,Kidney Transplantation ,Up-Regulation ,030104 developmental biology ,medicine.anatomical_structure ,Cohort ,Female ,business - Abstract
Accurate pre-transplant prediction of late graft function remains an unmet need in kidney transplantation. The aim of this study was to evaluate HLA genes expression levels in pre-implantation biopsies (PIB) of deceased donor kidneys as markers for long-term graft outcome.HLA genes expression analysis was initially performed using microarray data of 53 PIB, previously generated by our laboratory. The validation analysis was performed by real-time PCR in 116 PIB from an independent cohort.The microarray data showed association between high expression levels of HLA class II genes, especially HLA-DQB1 and -DQB2, in kidneys from young (18 to 49-year-old) donors and poor (eGFR 45 mL/min/1.73 mHeightened expression of HLA-DQB1 and -DQB2 in PIB are promising tools for pre-transplant risk assessment of poor late graft function in transplants with kidneys from 18 to 49-year-old donors.
- Published
- 2018