1. HT-2 toxin impairs porcine oocyte in vitro maturation through disruption of endomembrane system.
- Author
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Li JR, Wu SL, Hu LL, Liao BY, and Sun SC
- Subjects
- Animals, Swine, T-2 Toxin toxicity, T-2 Toxin analogs & derivatives, Female, Golgi Apparatus drug effects, Endoplasmic Reticulum drug effects, Mitochondria drug effects, Oocytes drug effects, In Vitro Oocyte Maturation Techniques veterinary
- Abstract
HT-2 toxin is a type of mycotoxin which is shown to affect gastric and intestinal lesions, hematopoietic and immunosuppressive effects, anorexia, lethargy, nausea. Recently, emerging evidences indicate that HT-2 also disturbs the reproductive system. In this study, we investigated the impact of HT-2 toxin exposure on the organelles of porcine oocytes. Our results found that the abnormal distribution of endoplasmic reticulum increased after HT-2 treatment, with the perturbation of ribosome protein RPS3 and GRP78 expression; Golgi apparatus showed diffused localization pattern and GM130 localization was also impaired, thereby affecting the Rab10-based vesicular transport; Due to the impairment of ribosomes, ER, and Golgi apparatus, the protein supply to lysosomes was hindered, resulting in lysosomal damage, which further disrupted the LC3-based autophagy. Moreover, the results indicated that the function and distribution of mitochondria were also affected by HT-2 toxin, showing with fragments of mitochondria, decreased TMRE and ATP level. Taken together, our study suggested that HT-2 toxin exposure induces damage to the organelles for endomembrane system, which further inhibited the meiotic maturation of porcine oocytes., Competing Interests: Declaration of competing interest The absence of any conflicts of interest in relation to this article has been explicitly acknowledged by the authors., (Copyright © 2024 Elsevier Inc. All rights reserved.)
- Published
- 2024
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