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11 results on '"Elise Bouffard"'

1. Efficient Photodynamic Therapy of Prostate Cancer Cells through an Improved Targeting of the Cation-Independent Mannose 6-Phosphate Receptor

Author

Elise Bouffard, Chiara Mauriello Jimenez, Khaled El Cheikh, Marie Maynadier, Ilaria Basile, Laurence Raehm, Christophe Nguyen, Magali Gary-Bobo, Marcel Garcia, Jean-Olivier Durand, and Alain Morère

Subjects
mannose 6-phosphate analogues, binding affinity, mesoporous silica nanoparticles, photodynamic therapy, Biology (General), QH301-705.5, Chemistry, QD1-999
Abstract

The aim of the present work is the development of highly efficient targeting molecules to specifically address mesoporous silica nanoparticles (MSNs) designed for the photodynamic therapy (PDT) of prostate cancer. We chose the strategy to develop a novel compound that allows the improvement of the targeting of the cation-independent mannose 6-phosphate receptor, which is overexpressed in prostate cancer. This original sugar, a dimannoside-carboxylate (M6C-Man) grafted on the surface of MSN for PDT applications, leads to a higher endocytosis and thus increases the efficacy of MSNs.

Published
2019
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2. Refinement of Covalent EGFR Inhibitor AZD9291 to Eliminate Off-target Activity

Author

Balyn W. Zaro, Elise Bouffard, Melissa M. Dix, Chi-Huey Wong, and Benjamin F. Cravatt

Subjects
Side effect, 010405 organic chemistry, Chemistry, Organic Chemistry, Disease, Drug resistance, 010402 general chemistry, Proteomics, medicine.disease, 01 natural sciences, Biochemistry, Article, 0104 chemical sciences, respiratory tract diseases, T790M, Drug Discovery, medicine, Cancer research, Lung cancer, Tyrosine kinase, EGFR inhibitors
Abstract

Non-small-cell lung cancer (NSCLC) is a major disease that accounts for 85% of all lung cancer cases which claimed around 1.8 billion lives worldwide in 2020. Tyrosine kinase inhibitors (TKIs) that target EGFR have been used for the treatment of NSCLC, but often develop drug resistance, and the covalent inhibitor AZD9291 has been developed to tackle the problem of drug resistance mediated by the T790M EGFR mutation; however, there is a side effect of hyperglycemia that may be due to off-target activity. This study examines analogs of AZD9291 by chemical proteomics, identifying analogs that maintain T790M-EGFR engagement while showing reduced cross-reactivity with off-targets.

Published
2021

3. Efficient Photodynamic Therapy of Prostate Cancer Cells through an Improved Targeting of the Cation-Independent Mannose 6-Phosphate Receptor

Author

Marcel Garcia, Jean-Olivier Durand, Marie Maynadier, Elise Bouffard, Laurence Raehm, Khaled El Cheikh, Chiara Mauriello Jimenez, Christophe Nguyen, Alain Morère, Magali Gary-Bobo, Ilaria Basile, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), and NanoMedSyn

Subjects
Male, mannose 6-phosphate analogues, medicine.medical_treatment, Mannose, Photodynamic therapy, 02 engineering and technology, 010402 general chemistry, Endocytosis, 01 natural sciences, Receptor, IGF Type 2, Catalysis, lcsh:Chemistry, Inorganic Chemistry, chemistry.chemical_compound, Prostate cancer, Cell Line, Tumor, binding affinity, medicine, Humans, [CHIM]Chemical Sciences, Physical and Theoretical Chemistry, Receptor, mesoporous silica nanoparticles, lcsh:QH301-705.5, Molecular Biology, Spectroscopy, Mannosephosphates, Communication, Organic Chemistry, Prostatic Neoplasms, General Medicine, Mesoporous silica, Silicon Dioxide, 021001 nanoscience & nanotechnology, medicine.disease, 0104 chemical sciences, Computer Science Applications, Cation-independent mannose-6 phosphate receptor, lcsh:Biology (General), lcsh:QD1-999, Photochemotherapy, chemistry, photodynamic therapy, Cancer research, Nanoparticles, 0210 nano-technology
Abstract

This article belongs to the Special Issue Insights into Photodynamic TherapyThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).; International audience; The aim of the present work is the development of highly efficient targeting molecules to specifically address mesoporous silica nanoparticles (MSNs) designed for the photodynamic therapy (PDT) of prostate cancer. We chose the strategy to develop a novel compound that allows the improvement of the targeting of the cation-independent mannose 6-phosphate receptor, which is overexpressed in prostate cancer. This original sugar, a dimannoside-carboxylate (M6C-Man) grafted on the surface of MSN for PDT applications, leads to a higher endocytosis and thus increases the efficacy of MSNs. View Full-TextKeywords: mannose 6-phosphate analogues; binding affinity; mesoporous silica nanoparticles; photodynamic therapy

Published
2019

4. Ruthenium(<scp>ii</scp>) complex-photosensitized multifunctionalized porous silicon nanoparticles for two-photon near-infrared light responsive imaging and photodynamic cancer therapy

Author

Marie Maynadier, Vanja Stojanovic, Alain Morère, Marcel Garcia, Jean-Olivier Durand, Elise Bouffard, Frédérique Cunin, Nikola Ž. Knežević, Gilles Lemercier, Khaled El Cheikh, Magali Gary-Bobo, Arnaud Chaix, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Materials science, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, medicine.medical_treatment, Biomedical Engineering, Nanoparticle, chemistry.chemical_element, Photodynamic therapy, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, 02 engineering and technology, Polyethylene glycol, 010402 general chemistry, Porous silicon, Photochemistry, 01 natural sciences, chemistry.chemical_compound, Two-photon excitation microscopy, medicine, Moiety, [CHIM.COOR]Chemical Sciences/Coordination chemistry, General Materials Science, technology, industry, and agriculture, General Chemistry, General Medicine, equipment and supplies, 021001 nanoscience & nanotechnology, 0104 chemical sciences, 3. Good health, Ruthenium, chemistry, Nanocarriers, 0210 nano-technology
Abstract

International audience; Multifunctionalized porous silicon nanoparticles (pSiNP) containing novel Ru(II) complex-photosensitizer, polyethylene glycol moiety, and mannose molecules as cancer targeting ligands, are constructed and showcased for application in near infrared (NIR) light-responsive photodynamic therapy (PDT) and imaging of cancer. Exposure to NIR light leads to twophoton excitation of the Ru(II)-complex which allows efficient simultaneous cancer-imaging and targeted PDT therapy with the functionalized biodegradable pSiNP nanocarriers.

Published
2016

5. Convenient Synthesis of the Protein Thermal-Stabilizer Mannosylglycerate

Author

Nadège Hamon, Alain Morère, Elise Bouffard, and Khaled El Cheikh

Subjects
Natural product, 010405 organic chemistry, General Chemistry, 010402 general chemistry, 01 natural sciences, Combinatorial chemistry, Mannosylglycerate, 0104 chemical sciences, chemistry.chemical_compound, chemistry, Thermal, medicine, Mannitol, Oxidative cleavage, medicine.drug, Stabilizer (chemistry)
Published
2016

6. Mesoporous silicon nanoparticles for targeted two-photon theranostics of prostate cancer

Author

Vanja Stojanovic, Alain Morère, Nikola Knezevic, Frédérique Cunin, Elise Bouffard, Sébastien Richeter, Arnaud Chaix, Philippe Maillard, Khaled El Cheikh, Dina Aggad, Magali Gary-Bobo, Marcel Garcia, Jean-Olivier Durand, Marie Maynadier, Laurence Raehm, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Nanomedsyn, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), NanoMedSyn, Distributed Programming Laboratory (LPD), Ecole Polytechnique Fédérale de Lausanne (EPFL), and Institut Curie, Research Center, PSL Research University

Subjects
Materials science, Silicon, medicine.medical_treatment, [SDV]Life Sciences [q-bio], Biomedical Engineering, Nanoparticle, chemistry.chemical_element, Photodynamic therapy, Nanotechnology, 02 engineering and technology, [CHIM.INOR]Chemical Sciences/Inorganic chemistry, 010402 general chemistry, Porous silicon, 01 natural sciences, Prostate cancer, Two-photon excitation microscopy, medicine, [CHIM]Chemical Sciences, General Materials Science, technology, industry, and agriculture, General Chemistry, General Medicine, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, medicine.disease, equipment and supplies, 0104 chemical sciences, chemistry, 0210 nano-technology, Mesoporous material
Abstract

International audience; A novel non-toxic porous silicon nanoparticle grafted with a mannose-6-phosphate analogue and applicable in 2-photon imaging and photodynamic therapy was specifically designed for targeting prostate cancer cells.

Published
2016

7. Why Anticancer Nanomedicine Needs Sugars?

Author

Ilaria Basile, K. El Cheikh, Elise Bouffard, Magali Gary-Bobo, Marie Maynadier, A. B. F. da Silva, Alain Morere, M. Garcia, and Audrey Gallud

Subjects
Pharmacology, chemistry.chemical_classification, Physicochemical Processes, Drug Carriers, Biocompatibility, Biomolecule, Organic Chemistry, Carbohydrates, Target tissue, Nanotechnology, Antineoplastic Agents, Biochemistry, Nanomedicine, chemistry, Drug Discovery, Molecular Medicine, Animals, Humans
Abstract

Nowadays, nanomedicine brings new opportunities for diagnosis and treatment through innovative combinations of materials structured at the nanoscale, biomolecules and physicochemical processes. If the intrinsic properties of nanomaterials appear of major importance in this new discipline, the functionalization of these nanotools with biomolecules improves both their biocompatibility and efficacy. This is the case of carbohydrate derivatives, natural or synthetic, which are increasingly being used in nanostructures for medical purposes. As in current medicine, sugars are used to mimic their physiological roles. Indeed, carbohydrates enhance the solubility and reduce the clearance of drugs. They are used to mask immunogenic components of nano-objects and escape the body defenses and finally facilitate the delivery to the target tissue. All these properties explain the growing importance of sugars in nanomedicine.

Published
2015

8. Cover Feature: Two-Photon Fluorescence Imaging and Therapy of Cancer Cells with Anisotropic Gold-Nanoparticle-Supported Porous Silicon Nanostructures (ChemNanoMat 4/2018)

Author

Arnaud Chaix, Jean-Olivier Durand, Frédéric Favier, Anthony Brocéro, Magali Gary-Bobo, Khaled El Cheikh, Elise Bouffard, Vanja Stojanovic, Marcel Garcia, Alain Morère, Frédérique Cunin, Marie Maynadier, and Khalil Rajoua

Subjects
Nanostructure, Materials science, Silicon, Renewable Energy, Sustainability and the Environment, Energy Engineering and Power Technology, chemistry.chemical_element, Nanoparticle, Nanotechnology, Porous silicon, Two photon fluorescence, Biomaterials, chemistry, Feature (computer vision), Materials Chemistry, Anisotropy
Published
2018

9. Two-Photon Excitation of Porphyrin-Functionalized Porous Silicon Nanoparticles for Photodynamic Therapy

Author

Jean-Olivier Durand, Marie Maynadier, Audrey Gallud, Marcel Garcia, Vincent Hugues, Elise Bouffard, Philippe Maillard, Mireille Blanchard-Desce, Emilie Secret, Céline Frochot, Alain Morère, Frédérique Cunin, Khaled El Cheikh, Michael J. Sailor, Mélanie Auffan, Magali Gary-Bobo, Olivier Mongin, Arnaud Chaix, Nathalie Marcotte, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Network Centric (NC), TRT - THALES RESEARCH & TECHNOLOGY, Centre européen de recherche et d'enseignement des géosciences de l'environnement (CEREGE), Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Collège de France (CdF (institution))-Institut de Recherche pour le Développement (IRD)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Conception, synthèse et vectorisation de biomolécules. (CSVB), Université Paris Descartes - Paris 5 (UPD5)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris], Chimie et Photonique Moléculaires (CPM), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Dept Chem & Biochem UC San Diego, University of California [San Diego] (UC San Diego), University of California-University of California, Institut de recherche en cancérologie de Montpellier (IRCM - U896 Inserm - UM1), CRLCC Val d'Aurelle - Paul Lamarque-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 1 (UM1), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Réactions et Génie des Procédés (LRGP), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes - Paris 5 (UPD5)-Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Department of Chemistry and Biochemistry [Univ California San Diego] (Chemistry - UC San Diego), University of California (UC)-University of California (UC), Université Montpellier 1 (UM1)-CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Collège de France (CdF)-Institut national des sciences de l'Univers (INSU - CNRS)-Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA), Institut Curie-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5), Univ Calif San Diego, Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Rennes-Université de Rennes 1 (UR1), and Université de Rennes (UNIV-RENNES)

Subjects
Silicon, Porphyrins, Materials science, Infrared Rays, medicine.medical_treatment, Nanoparticle, Biocompatible Materials, Photodynamic therapy, 02 engineering and technology, 010402 general chemistry, Porous silicon, Endocytosis, Photochemistry, 01 natural sciences, chemistry.chemical_compound, Two-photon excitation microscopy, medicine, Humans, [CHIM]Chemical Sciences, General Materials Science, Nir laser, ComputingMilieux_MISCELLANEOUS, Photons, Microscopy, Confocal, Photosensitizing Agents, Cell Death, Mechanical Engineering, technology, industry, and agriculture, equipment and supplies, 021001 nanoscience & nanotechnology, Porphyrin, 0104 chemical sciences, 3. Good health, Microscopy, Fluorescence, Photochemotherapy, chemistry, Mechanics of Materials, MCF-7 Cells, Nanoparticles, 0210 nano-technology, Mannose, Porosity, Excitation
Abstract

Porous silicon nanoparticles (pSiNPs) act as a sensitizer for the 2-photon excitation of a pendant porphyrin using NIR laser light, for imaging and photodynamic therapy. Mannose-functionalized pSiNPs can be vectorized to MCF-7 human breast cancer cells through a mannose receptor-mediated endocytosis mechanism to provide a 3-fold enhancement of the 2-photon PDT effect.

Published
2014

10. Targeting multiplicity: the key factor for anti-cancer nanoparticles

Author

X. Rebillard, Elise Bouffard, Ophélie Vaillant, Philippe Nirdé, Audrey Gallud, Ilaria Basile, Marie Maynadier, Magali Gary-Bobo, K. El Cheikh, Alain Morere, M. Garcia, P. Puche, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), and Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)

Subjects
Antineoplastic Agents, Nanotechnology, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Biochemistry, non-invasive therapy, Drug Delivery Systems, multiple targeting, Neoplasms, Drug Discovery, Tumor Microenvironment, Animals, Humans, Medicine, Cancer, Pharmacology, Tumor microenvironment, business.industry, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Oxygen metabolism, Organic Chemistry, Non invasive, Life time, 021001 nanoscience & nanotechnology, 3. Good health, 0104 chemical sciences, Oxygen, Cancer cell, Cancer research, Molecular Medicine, nanoparticles, 0210 nano-technology, business
Abstract

International audience; In this mini-review, we focus on different strategies to bring nanotools specifically to cancer cells. We discuss about a better targeting of tumor, combining the characteristics of tumor environment, the increase in nanoparticles life time, the biomarkers overexpressed on cancer cells and different physical methods for non invasive therapies. Here we detail the necessity of a synergy between passive and active targeting for an actual specificity of cancer cells.

Published
2013

11. Porphyrin-Functionalized Porous Silicon Nanoparticles for Photodynamic Therapy

Author

Emilie Secret, Marie Maynadier, Audrey Gallud, Arnaud Chaix, Elise Bouffard, Magali Gary-Bobo, Nathalie Marcotte, Olivier Mongin, Khaled El Cheikh, Vincent Hugues, Melanie Auffan, Céline Frochot, Alain Morère, Philippe Maillard, Mireille Blanchard-Desce, M. J. Sailor, Marcel Garcia, Jean-Olivier Durand, and Frederique Cunin

Abstract

Nanomaterials offer the potential for minimally invasive and focal therapies to reduce side-effects associated with systemic administration of anticancer drugs. Clinical treatment involving photodynamic therapy is particularly attractive since it allows spatial and temporal control of the production of highly cytotoxic singlet oxygen and reactive oxygen species through local excitation of a photosensitizer. Porous silicon nanoparticles (pSiNP), obtained by electrochemical etching of crystalline silicon followed by ultrasonic treatment, are particularly attractive for nanomedicine because they are bioresorbable in vivo and their degradation product, silicic acid, is non-toxic. In addition pSiNP display intrinsic optical properties (luminescence) deriving from their semi-conductive nature, very usefull for imaging. Here we will present simple photosensitizer/pSiNP systems based on a porphyrin derivative covalently attached to the nanoparticle, and demonstrate imaging and PDT in vitro under one (OPE) and two photon excitation (TPE) conditions. We will describe the preparation of the biodegradable pSiNP, the chemical functionalization of pSiNP with the photosensitizer by the covalent anchoring of a porphyrin into the porous silicon matrix using a new type of conjugation chemistry, as well as their functionalization with cancer cell targeting species. The targeting and photodynamic efficiency of the described formulations on various cancer cell lines will be presented. We will show that both types of excitation, OPE and TPE, lead to cell death through different excitation pathways. Emilie Secret, Marie Maynadier, Audrey Gallud, Arnaud Chaix, Elise Bouffard, Magali Gary-Bobo, Nathalie Marcotte, Olivier Mongin, Khaled El Cheikh, Vincent Hugues, Mélanie Auffan, Céline Frochot, Alain Morère, Philippe Maillard, Mireille Blanchard-Desce, Michael J. Sailor, Marcel Garcia, Jean-Olivier Durand and Frédérique Cunin,Two-Photon Excitation of Porphyrin Functionalized Porous Silicon Nanoparticles for Photodynamic Therapy Adv. Mater., 2014, Online. DOI: 10.1002/adma.201403415. Emilie Secret, Marie Maynadier, Audrey Gallud, Magali Gary-Bobo, Arnaud Chaix, Emmanuel Belamie, Philippe Maillard, Michael J. Sailor, Marcel Garcia, Jean-Olivier Durand, Frédérique Cunin, Anionic porphyrin-grafted porous silicon nanoparticles for photodynamic therapy,Chem. Commun., 2013, 49(39), 4202-4204. DOI: 10.1039/C3CC38837A

Published
2015

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