68 results on '"Elisabeth Plagnes-Juan"'
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2. On the Utilization of Dietary Glycerol in Carnivorous Fish—Part II: Insights Into Lipid Metabolism of Rainbow Trout (Oncorhynchus mykiss) and European Seabass (Dicentrarchus labrax)
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Ivan Viegas, Mariana Palma, Elisabeth Plagnes-Juan, Emanuel Silva, João Rito, Luís F. Henriques, Ludgero C. Tavares, Rodrigo O. A. Ozório, Stéphane Panserat, and Leonardo J. Magnoni
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2H NMR ,aquaculture ,circular economy ,deuterated water ,glycerol ,muscle ,Science ,General. Including nature conservation, geographical distribution ,QH1-199.5 - Abstract
Glycerol is the by-product of biodiesel production and its utilisation in fish feed has recently gained relevance. As an important metabolic intermediate and structural component of triacylglycerol (TAG), it is still unclear whether its supplementation affects lipid utilisation and/or deposition in different tissues. Accordingly, a set of studies was aimed to evaluate how increasing levels of dietary glycerol (0, 2.5 and 5%) affect lipid synthesis in the liver, muscle and adipose tissue. After a growth trial with rainbow trout (8 weeks) and European seabass (6 weeks) fish were sampled at 6 and 24 h to assess mRNA levels of lipid metabolism-related enzymes. The remaining fish were subjected to a metabolic trial consisting of a 6-day residence in deuterated water (2H2O) for metabolic flux calculations. This study stands as the second part of a broader experiment that also aimed at evaluating the carbohydrate metabolism (Viegas et al., 2022). Dietary supplementation at 5% glycerol significantly increased plasma TAG levels in both species and liver TAG levels in seabass, with no repercussions on the perivisceral fat index. Despite responding as expected in a postprandial setting, only fas and Δ6-fad in trout and Δ6-fad in seabass responded significantly by increasing with the dietary supplementation of glycerol. In trout, the observed differences in the regulation of these enzymes were not reflected in the de novo lipogenic fluxes. The fractional synthetic rates (FSR) were overall low in all tissues with an average of 0.04, 0.02 and 0.01% d–1, for liver, muscle and perivisceral fat, respectively. In seabass on the other hand, and despite increased mRNA levels in Δ6-fad, the overall lipid profile in the liver muscle and perivisceral fat was higher in MUFA at the expense of lower PUFA. Moreover, glycerol supplementation altered the lipogenic capacity of seabass with hepatic fractional synthetic rates for TAG-bound FA increasing with increasing glycerol levels (0.32 ± 0.18, 0.57 ± 0.18, and 0.82 ± 0.24 for 0%, 2.5% and 5% glycerol supplementation, respectively). The findings of the present study suggest that supplementation up to 2.5% of glycerol did not severely impact the lipid metabolism nor increased lipogenic potential in liver, muscle and perivisceral fat accumulation.
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- 2022
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3. On the Utilization of Dietary Glycerol in Carnivorous Fish - Part I: Insights Into Hepatic Carbohydrate Metabolism of Juvenile Rainbow Trout (Oncorhynchus mykiss) and European Seabass (Dicentrarchus labrax)
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Ivan Viegas, Ludgero C. Tavares, Elisabeth Plagnes-Juan, Emanuel Silva, João Rito, Lucie Marandel, Mariana Palma, Rodrigo O. A. Ozório, Leonardo J. Magnoni, and Stéphane Panserat
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2H NMR ,aquaculture ,blood glucose ,circular economy ,deuterated water ,glycerol ,Science ,General. Including nature conservation, geographical distribution ,QH1-199.5 - Abstract
Glycerol is a 3-carbon sugar alcohol successfully employed as an alternative feed ingredient for land-farmed animals and more recently for farmed fish. While most studies address zootechnical performance, few have delved into the metabolic utilization of dietary glycerol. A growth trial was performed using diets with increasing levels of glycerol (0, 2.5 and 5%) on two relevant species for aquaculture: rainbow trout (8-week trial; 3 tank per diet/25 fish per tank, on a 15 ± 1°C flow-through freshwater system); and European seabass (6-week trial; 5 tank per diet/6-8 fish per tank on a 21°C indoor saltwater RAS system). After this period, fish were subjected to a metabolic trial consisting of a 6-day residence in deuterated water (2H2O). Measurements of blood glucose and hepatic glycogen 2H-enrichments through Nuclear Magnetic Resonance, complemented by mRNA levels of key-enzymes for intermediary metabolism were used to evaluate the catabolic pathways of dietary glycerol. Dietary glycerol had no impact on plasma glucose, but hepatic glycogen levels increased significantly with increasing dietary glycerol levels in both species. While trout was able to regulate circulating glycerol plasma, seabass presented elevated levels on the glycerol-supplemented diets. Despite revealing some significant differences between sampling time (6 and 24 h), none of the enzymes’ mRNA levels responded to the dietary treatment. In trout, the main source of blood glucose was not labeled with 2H (~60%, likely from diet) while other contributors did not differ with glycerol supplementation. In seabass, the unlabeled contribution was approximately half of that observed in trout (~30%), accompanied by a significant increase of gluconeogenic contributions at the triose-phosphate level to the blood glucose with increasing dietary glycerol. In trout, labeling from 2H2O into hepatic glycogen revealed significant differences, with the contribution from the indirect pathway at the triose-phosphate level increasing with increased dietary glycerol. No such differences were found in seabass’ glycogen pool. These findings suggest that fish species are able to retain, catabolize glycerol and incorporate it into carbohydrates. The gluconeogenic utilization of exogenous glycerol differed between species and affected the synthesis of hepatic glycogen in trout and the appearance of blood glucose in seabass.
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- 2022
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4. Hepatic Glycerol Metabolism-Related Genes in Carnivorous Rainbow Trout (Oncorhynchus mykiss): Insights Into Molecular Characteristics, Ontogenesis, and Nutritional Regulation
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Stephane Panserat, Elisabeth Plagnes-Juan, Elsa Gazzola, Mariana Palma, Leonardo J. Magnoni, Lucie Marandel, and Ivan Viegas
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glycerol metabolism ,fish nutrition ,genomics ,gene expression ,rainbow trout ,aquaculture ,Physiology ,QP1-981 - Abstract
Glycerol metabolism in rainbow trout is poorly studied even though it is at the interface between lipid and glucose metabolism. Moreover, glycerol can be an important ingredient in new aquafeed formulation to decrease the catabolism of dietary amino acids. Thus, the present study aimed to characterize for the first time the different genes coding for key enzymes and proteins involved in hepatic glycerol metabolism. From the trout genomes, all the paralogous genes coding for glycerol transport (aqp9b), glycerol kinase (gk2a and gk5), glycerol-3-phosphate phosphatase (pgp), and glycerol-3-phosphate dehydrogenase (gpd1a, gpd1b, and gpd1c) were identified. The ontogenesis determined that the capacity to metabolize glycerol begins with the apparition of the liver during the development (stage 22) and are more expressed at the endogenous–exogenous feeding period (stage 35). The postprandial regulation of the expression of these genes in juvenile trout showed that the postprandial peak of expression is between 4 and 24 h after the last meal for many of the genes, demonstrating that glycerol metabolism could be nutritionally regulated at a molecular level. However, surprisingly, no regulation of the mRNA abundance for the glycerol metabolism-related genes by different levels of dietary glycerol (0, 2.5, and 5%) have been detected, showing that hepatic glycerol metabolism is poorly regulated at a molecular level by dietary glycerol in rainbow trout juveniles.
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- 2020
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5. Exploring the Impact of a Low-Protein High-Carbohydrate Diet in Mature Broodstock of a Glucose-Intolerant Teleost, the Rainbow Trout
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Thérèse Callet, Huihua Hu, Laurence Larroquet, Anne Surget, Jingwei Liu, Elisabeth Plagnes-Juan, Patrick Maunas, Nicolas Turonnet, Jan Alexander Mennigen, Julien Bobe, Christine Burel, Geneviève Corraze, Stephane Panserat, and Lucie Marandel
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liver ,gonad ,enzymatic activity ,glucose metabolism ,pentose phosphate pathway ,glycogen ,Physiology ,QP1-981 - Abstract
Sustainable aquaculture production requires a greater reduction in the use of marine-derived ingredients, and one of the most promising solutions today is the augmentation in the proportion of digestible carbohydrates in aquafeed. This challenge is particularly difficult for high trophic level teleost fish as they are considered to be glucose-intolerant (growth delay and persistent postprandial hyperglycemia observed in juveniles fed a diet containing more than 20% of carbohydrates). It was previously suggested that broodstock could potentially use carbohydrates more efficiently than juveniles, probably due to important metabolic changes that occur during gametogenesis. To investigate this hypothesis, 2-year old male and female rainbow trout (Oncorhynchus mykiss) were either fed a diet containing no carbohydrates (NC) or a 35%-carbohydrate diet (HC) for an entire reproductive cycle. Zootechnical parameters as well as the activities of enzymes involved in carbohydrate metabolism were measured in livers and gonads. Fish were then reproduced to investigate the effects of such a diet on reproductive performance. Broodstock consumed the HC diet, and in contrast to what is commonly observed in juveniles, they were able to grow normally and they did not display postprandial hyperglycemia. The modulation of their hepatic metabolism, with an augmentation of the glycogenesis, the pentose phosphate pathway and a possible better regulation of gluconeogenesis, may explain their improved ability to use dietary carbohydrates. Although the HC diet did induce precocious maturation, the reproductive performance of fish was not affected, confirming that broodstock are able to reproduce when fed a low-protein high-carbohydrate diet. In conclusion, this exploratory work has shown that broodstock are able to use a diet containing digestible carbohydrates as high as 35% and can then grow and reproduce normally over an entire reproductive cycle for females and at least at the beginning of the cycle for males. These results are highly promising and suggest that dietary carbohydrates can at least partially replace proteins in broodstock aquafeed.
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- 2020
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6. Glucose Injection Into Yolk Positively Modulates Intermediary Metabolism and Growth Performance in Juvenile Nile Tilapia (Oreochromis niloticus)
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Suksan Kumkhong, Lucie Marandel, Elisabeth Plagnes-Juan, Vincent Veron, Surintorn Boonanuntanasarn, and Stephane Panserat
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nile tilapia ,nutritional programming ,glucose injection ,gene expression ,glucose metabolism ,Physiology ,QP1-981 - Abstract
The aim of this study was to explore for the first time in omnivorous fish the concept of nutritional programming. A nutritional stimulus was accomplished by microinjecting 2 M glucose into yolk reserves during the alevin stage in Nile tilapia (Oreochromis niloticus). At the molecular level in fry, at 1 week post-injection, glucose stimuli were associated with the up-regulation of genes involved in glycolysis (pklr, hk1, hk2, and pkma), glucose transport (glut4) pathways and down-regulation of genes related to gluconeogenesis (g6pca1, g6pca2, and pck1) and amino acid catabolism (asat, alat) (P < 0.05), demonstrating that the larvae well received the glucose stimulus at a molecular level. Moreover, 20 weeks after glucose injection, early glucose stimuli were always linked to permanent effects in juvenile fish, as reflected by a higher level of glycolytic enzymes [gck, hk1 and hk2 at both mRNA and enzymatic levels and pyruvate kinase (PK) activity]. Finally, the effects of the glucose stimulus history were also examined in fish fed with two different dietary carbohydrate/protein levels (medium-carbohydrate diet, CHO-M; high-carbohydrate diet, CHO-H) in juvenile fish (during weeks 20–24). As expected, the CHO-H diet induced the expression of glycolytic and lipogenic genes (gck, pklr, hk1, hk2, fpkma, fasn, and g6pd) and suppressed the expression of gluconeogenic and amino acid catabolism genes (g6pca1, pck1, pck2, asat, alat, and gdh). Nevertheless, the early glucose stimulus led to persistent up-regulation of glycolytic enzymes (gck, pklr, hk1, and hk2) at both the mRNA and enzyme activity levels and glucose transporter glut4 as well as lower gluconeogenic pck1 gene expression (P < 0.05). More interestingly, the early glucose stimulus was associated with a better growth performance of juvenile fish irrespective of the diets. These permanent changes were associated with DNA hypomethylation in the liver and muscles, suggesting the existence of epigenetic mechanisms at the origin of programming. In conclusion, for the first time in tilapia, early glucose stimuli were found to be clearly associated with a positive metabolic programming effect later in life, improving the growth performance of the fish.
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- 2020
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7. Nutritional regulation of glucose metabolism-related genes in the emerging teleost model Mexican tetra surface fish: a first exploration
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Lucie Marandel, Elisabeth Plagnes-Juan, Michael Marchand, Therese Callet, Karine Dias, Frederic Terrier, Stéphane Père, Louise Vernier, Stephane Panserat, and Sylvie Rétaux
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mexican tetra ,gluconeogenesis ,nutrition ,brain ,liver ,Science - Abstract
Astyanax mexicanus has gained importance as a laboratory model organism for evolutionary biology. However, little is known about its intermediary metabolism, and feeding regimes remain variable between laboratories holding this species. We thus aimed to evaluate the intermediary metabolism response to nutritional status and to low (NC) or high (HC) carbohydrate diets in various organs of the surface-dwelling form of the species. As expected, glycaemia increased after feeding. Fish fed the HC diet had higher glycaemia than fish fed the NC diet, but without displaying hyperglycaemia, suggesting that carbohydrates are efficiently used as an energy source. At molecular level, only fasn (Fatty Acid Synthase) transcripts increased in tissues after refeeding, suggesting an activation of lipogenesis. On the other hand, we monitored only moderate changes in glucose-related transcripts. Most changes observed were related to the nutritional status, but not to the NC versus HC diet. Such a metabolic pattern is suggestive of an omnivorous-related metabolism, and this species, at least at adult stage, may adapt to a fish meal-substituted diet with high carbohydrate content and low protein supply. Investigation to identify molecular actors explaining the efficient use of such a diet should be pursued to deepen our knowledge on this species.
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- 2020
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8. Early feeding with hyperglucidic diet during fry stage exerts long-term positive effects on nutrient metabolism and growth performance in adult tilapia (Oreochromis niloticus)
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Suksan Kumkhong, Lucie Marandel, Elisabeth Plagnes-Juan, Vincent Veron, Stephane Panserat, and Surintorn Boonanuntanasarn
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Nile tilapia ,Nutrient programming ,Growth performance ,Early feeding ,Gene expression ,Glucose metabolism ,Nutrition. Foods and food supply ,TX341-641 ,Medicine - Abstract
The present study aimed to investigate nutritional programming of carbohydrate metabolism in Nile tilapia. Early nutritional intervention stimulus was achieved by feeding fry with high-protein/low-carbohydrate (HP/LC) or low-protein/high-carbohydrate (LP/HC) diet since first feeding for 4 weeks, and the effect of nutritional stimulus on carbohydrate and its related metabolism was evaluated through the adult stage. Our findings indicated that at week 1, LP/HC diet-fed fry had lower levels of mRNA for genes coding gluconeogenesis and amino acid catabolism and higher levels of hk2 (P < 0⋅05). As expected, in adult tilapia, although LP/HC diet-fed fish had poorer growth (end of stimulus), the fish showed compensatory growth. There were permanent effects of early high-carbohydrate (HC) intake on several parameters, including (1) modulating hepatic composition, (2) increased muscle glycogen, (3) lower levels of enzymes involved in amino acid catabolism and (4) higher levels of glycolytic enzymes in glycolysis. Finally, HP/LC diet- and LP/HC diet-fed fish were challenged with different dietary carbohydrate levels. Irrespective of challenging diets, the early HC stimulus had significant effects on adult tilapia by (1) promoting utilisation of glucose, which had protein-sparing effects for better growth, (2) inducting lipogenesis and (3) decreasing amino acid catabolism. Taken together, for the first time, we demonstrated that early HC feeding was effective for positive nutritional programming of metabolism in Nile tilapia (an omnivorous fish). It led to the improvement of growth performance in adult fish associated with early feeding, which is linked to a better ability to use glucose, to induce lipogenesis, and to suppress amino acid catabolism.
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- 2020
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9. Cholesterol metabolism regulation mediated by SREBP-2, LXRα and miR-33a in rainbow trout (Oncorhynchus mykiss) both in vivo and in vitro.
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Tengfei Zhu, Geneviève Corraze, Elisabeth Plagnes-Juan, and Sandrine Skiba-Cassy
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Medicine ,Science - Abstract
Cholesterol metabolism is greatly affected in fish fed plant-based diet. The regulation of cholesterol metabolism is mediated by both transcriptional factors such as sterol regulatory element-binding proteins (SREBPs) and liver X receptors (LXRs), and posttranscriptional factors including miRNAs. In mammals, SREBP-2 and LXRα are involved in the transcriptional regulation of cholesterol synthesis and elimination, respectively. In mammals, miR-33a is reported to directly target genes involved in cholesterol catabolism. The present study aims to investigate the regulation of cholesterol metabolism by SREBP-2 and LXRα and miR-33a in rainbow trout using in vivo and in vitro approaches. In vivo, juvenile rainbow trout of ~72 g initial body weight were fed a total plant-based diet (V) or a marine diet (M) containing fishmeal and fish oil. In vitro, primary cell culture hepatocytes were stimulated by graded concentrations of 25-hydroxycholesterol (25-HC). The hepatic expression of cholesterol synthetic genes, srebp-2 and miR-33a as well as miR-33a level in plasma were increased in fish fed the plant-based diet, reversely, their expression in hepatocytes were inhibited with the increasing 25-HC in vitro. However, lxrα was not affected neither in vivo nor in vitro. Our results suggest that SREBP-2 and miR-33a synergistically enhance the expression of cholesterol synthetic genes but do not support the involvement of LXRα in the regulation of cholesterol elimination. As plasma level of miR-33a appears as potential indicator of cholesterol synthetic capacities, this study also highlights circulating miRNAs as promising noninvasive biomarker in aquaculture.
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- 2020
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10. Evolutionary history of glucose-6-phosphatase encoding genes in vertebrate lineages: towards a better understanding of the functions of multiple duplicates
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Lucie Marandel, Stéphane Panserat, Elisabeth Plagnes-Juan, Eva Arbenoits, José Luis Soengas, and Julien Bobe
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Teleosts ,Duplicated genes ,Sarcopterygii ,Actinopterygii ,trout ,glucose ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Glucose-6-phosphate (G6pc) is a key enzyme involved in the regulation of the glucose homeostasis. The present study aims at revisiting and clarifying the evolutionary history of g6pc genes in vertebrates. Results g6pc duplications happened by successive rounds of whole genome duplication that occurred during vertebrate evolution. g6pc duplicated before or around Osteichthyes/Chondrichthyes radiation, giving rise to g6pca and g6pcb as a consequence of the second vertebrate whole genome duplication. g6pca was lost after this duplication in Sarcopterygii whereas both g6pca and g6pcb then duplicated as a consequence of the teleost-specific whole genome duplication. One g6pca duplicate was lost after this duplication in teleosts. Similarly one g6pcb2 duplicate was lost at least in the ancestor of percomorpha. The analysis of the evolution of spatial expression patterns of g6pc genes in vertebrates showed that all g6pc were mainly expressed in intestine and liver whereas teleost-specific g6pcb2 genes were mainly and surprisingly expressed in brain and heart. g6pcb2b, one gene previously hypothesised to be involved in the glucose intolerant phenotype in trout, was unexpectedly up-regulated (as it was in liver) by carbohydrates in trout telencephalon without showing significant changes in other brain regions. This up-regulation is in striking contrast with expected glucosensing mechanisms suggesting that its positive response to glucose relates to specific unknown processes in this brain area. Conclusions Our results suggested that the fixation and the divergence of g6pc duplicated genes during vertebrates’ evolution may lead to adaptive novelty and probably to the emergence of novel phenotypes related to glucose homeostasis.
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- 2017
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11. Exposure to an acute hypoxic stimulus during early life affects the expression of glucose metabolism-related genes at first-feeding in trout
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Jingwei Liu, Elisabeth Plagnes-Juan, Inge Geurden, Stéphane Panserat, and Lucie Marandel
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Medicine ,Science - Abstract
Abstract Rainbow trout (Oncorhynchus mykiss) is considered a “glucose-intolerant” species. With the aim of programming trout to improve their metabolic use of dietary carbohydrates, we hypothesised that a hypoxic stimulus applied during embryogenesis could later affect glucose metabolism at the first-feeding stage. An acute hypoxic stimulus (2.5 or 5.0 mg·L−1 O2) was applied for 24 h to non-hatched embryos or early hatched alevins followed by a challenge test with a high carbohydrate diet at first-feeding. The effectiveness of the early hypoxic stimulus was confirmed by the induction of oxygen-sensitive markers such as egln3. At first-feeding, trout previously subjected to the 2.5 mg·L−1 O2 hypoxia displayed a strong induction of glycolytic and glucose transport genes, whereas these glucose metabolism-related genes were affected much less in trout subjected to the less severe (5.0 mg·L−1 O2) hypoxia. Our results demonstrate that an acute hypoxic stimulus during early development can affect glucose metabolism in trout at first-feeding.
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- 2017
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12. The Autophagic Flux Inhibitor Bafilomycine A1 Affects the Expression of Intermediary Metabolism-Related Genes in Trout Hepatocytes
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Sarah Séité, Tracy Pioche, Nicolas Ory, Elisabeth Plagnes-Juan, Stéphane Panserat, and Iban Seiliez
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fish ,hepatocyte ,autophagy ,intermediary metabolism ,ER stress ,gene expression ,Physiology ,QP1-981 - Abstract
Autophagy is an evolutionarily conserved process of cellular self-eating which emerged these last years as a major adaptive metabolic response to various stresses such as fasting, hypoxia, or environmental pollutants. However, surprisingly very few data is currently available on its role in fish species which are directly exposed to frequent environmental perturbations. Here, we report that the treatment of fasted trout hepatocytes with the autophagy inhibitor Bafilomycine A1 lowered the mRNA levels of many of the gluconeogenesis-related genes and increased those of genes involved in intracellular lipid stores. Concurrently, intracellular free amino acid levels dropped and the expression of the main genes involved in the endoplasmic reticulum (ER) stress exhibited a sharp increase in autophagy inhibited cells. Together these results highlight the strong complexity of the crosstalk between ER, autophagy and metabolism and support the importance of considering this function in future studies on metabolic adaptation of fish to environmental stresses.
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- 2019
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13. Circulating miRNA measurements are reflective of cholesterol-based changes in rainbow trout (Oncorhynchus mykiss).
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Tengfei Zhu, Geneviève Corraze, Elisabeth Plagnes-Juan, and Sandrine Skiba-Cassy
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Medicine ,Science - Abstract
MicroRNAs (miRNAs) are a class of small non-coding RNAs which are known to posttranscriptionally regulate the expression of most genes in both animals and plants. Meanwhile, studies have shown that numbers of miRNAs are present in body fluids including the plasma. Despite the mode of action of these circulating miRNAs still remains unknown, they have been found to be promising biomarkers for disease diagnosis, prognosis and response to treatment. In order to evaluate the potential of miRNAs as non-invasive biomarkers in aquaculture, a time-course experiment was implemented to investigate the postprandial regulation of miRNAs levels in liver and plasma as well as the hepatic expression of genes involved in cholesterol metabolism. We showed that miR-1, miR-33a, miR-122, miR-128 and miR-223 were expressed in the liver of rainbow trout and present at detectable level in the plasma. We also demonstrated that hepatic expression of miR-1, miR-122 and miR-128 were regulated by feed intake and reached their highest levels 12 hours after the meal. Interestingly, we observed that circulating levels of miR-128 and miR-223 are subjected to postprandial regulations similar to that observed in their hepatic counterparts. Statistical correlations were observed between liver and plasma for miR-128 and miR-223 and between hepatic and circulating miR-122, miR-128 and miR-223 and expression of genes related to cholesterol synthesis and efflux or glucose phosphorylation. These results demonstrated that circulating miR-122, miR-128 and miR-223 are potential biomarkers of cholesterol metabolism in rainbow trout.
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- 2018
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14. Amino Acids Attenuate Insulin Action on Gluconeogenesis and Promote Fatty Acid Biosynthesis via mTORC1 Signaling Pathway in trout Hepatocytes
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Weiwei Dai, Stéphane Panserat, Elisabeth Plagnes-Juan, Iban Seiliez, and Sandrine Skiba-Cassy
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mTORC1 ,Insulin action ,Fatty acid biosynthesis ,Gluconeogenesis ,Rainbow trout ,Amino acids ,Physiology ,QP1-981 ,Biochemistry ,QD415-436 - Abstract
Background/Aims: Carnivores exhibit poor utilization of dietary carbohydrates and glucose intolerant phenotypes, yet it remains unclear what are the causal factors and underlying mechanisms. We aimed to evaluate excessive amino acids (AAs)-induced effects on insulin signaling, fatty acid biosynthesis and glucose metabolism in rainbow trout and determine the potential involvement of mTORC1 and p38 MAPK pathway. Methods: We stimulated trout primary hepatocytes with different AA levels and employed acute administration of rapamycin to inhibit mTORC1 activation. Results: Increased AA levels enhanced the phosphorylation of ribosomal protein S6 kinase (S6K1), S6, and insulin receptor substrate 1 (IRS-1) on Ser302 but suppressed Akt and p38 phosphorylation; up-regulated the expression of genes related to gluconeogenesis and fatty acid biosynthesis. mTORC1 inhibition not only inhibited the phosphorylation of mTORC1 downstream targets, but also blunted IRS-1 Ser302 phosphorylation and restored excessive AAs-suppressed Akt phosphorylation. Rapamycin also inhibited fatty acid biosynthetic and gluconeogenic gene expression. Conclusion: High levels of AAs up-regulate hepatic fatty acid biosynthetic gene expression through an mTORC1-dependent manner, while attenuate insulin-mediated repression of gluconeogenesis through elevating IRS-1 Ser302 phosphorylation, which in turn impairs Akt activation and thereby weakening insulin action. We propose that p38 MAPK probably also involves in these AAs-induced metabolic changes.
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- 2015
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15. Postprandial regulation of hepatic microRNAs predicted to target the insulin pathway in rainbow trout.
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Jan A Mennigen, Stéphane Panserat, Mélanie Larquier, Elisabeth Plagnes-Juan, Françoise Medale, Iban Seiliez, and Sandrine Skiba-Cassy
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Medicine ,Science - Abstract
Rainbow trout are carnivorous fish and poor metabolizers of carbohydrates, which established this species as a model organism to study the comparative physiology of insulin. Following the recent characterisation of key roles of several miRNAs in the insulin action on hepatic intermediary metabolism in mammalian models, we investigated the hypothesis that hepatic miRNA expression is postprandially regulated in the rainbow trout and temporally coordinated in the context of insulin-mediated regulation of metabolic gene expression in the liver. To address this hypothesis, we used a time-course experiment in which rainbow trout were fed a commercial diet after short-term fasting. We investigated hepatic miRNA expression, activation of the insulin pathway, and insulin regulated metabolic target genes at several time points. Several miRNAs which negatively regulate hepatic insulin signaling in mammalian model organisms were transiently increased 4 h after the meal, consistent with a potential role in acute postprandial negative feed-back regulation of the insulin pathway and attenuation of gluconeogenic gene expression. We equally observed a transient increase in omy- miRNA-33 and omy-miRNA-122b 4 h after feeding, whose homologues have potent lipogenic roles in the liver of mammalian model systems. A concurrent increase in the activity of the hepatic insulin signaling pathway and the expression of lipogenic genes (srebp1c, fas, acly) was equally observed, while lipolytic gene expression (cpt1a and cpt1b) decreased significantly 4 h after the meal. This suggests lipogenic roles of omy-miRNA-33 and omy-miRNA-122b may be conserved between rainbow trout and mammals and that these miRNAs may furthermore contribute to acute postprandial regulation of de novo hepatic lipid synthesis in rainbow trout. These findings provide a framework for future research of miRNA regulation of hepatic metabolism in trout and will help to further elucidate the metabolic phenotype of rainbow trout.
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- 2012
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16. The metabolic consequences of hepatic AMP-kinase phosphorylation in rainbow trout.
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Sergio Polakof, Stéphane Panserat, Paul M Craig, David J Martyres, Elisabeth Plagnes-Juan, Sharareh Savari, Stéphane Aris-Brosou, and Thomas W Moon
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Medicine ,Science - Abstract
AMP-activated protein kinase (AMPK), a phylogenetically conserved serine/threonine protein kinase, is proposed to function as a "fuel gauge" to monitor cellular energy status in response to nutritional environmental variations. However, in fish, few studies have addressed the metabolic consequences related to the activation of this kinase. This study demonstrates that the rainbow trout (Oncorhynchus mykiss) possesses paralogs of the three known AMPK subunits that co-diversified, that the AMPK protein is present in the liver and in isolated hepatocytes, and it does change in response to physiological (fasting-re-feeding cycle) and pharmacological (AICAR and metformin administration and incubations) manipulations. Moreover, the phosphorylation of AMPK results in the phosphorylation of acetyl-CoA carboxylase, a main downstream target of AMPK in mammals. Other findings include changes in hepatic glycogen levels and several molecular actors involved in hepatic glucose and lipid metabolism, including mRNA transcript levels for glucokinase, glucose-6-phosphatase and fatty acid synthase both in vivo and in vitro. The fact that most results presented in this study are consistent with the recognized role of AMPK as a master regulator of energy homeostasis in living organisms supports the idea that these functions are conserved in this piscine model.
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- 2011
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17. Short initial period of high carbohydrate feeding improves nutrient utilisation in juvenile Nile tilapia (Oreochromis niloticus) fed a high carbohydrate diet
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Nattanan Srisakultiew, Suksan Kumkhong, Lucie Marandel, Elisabeth Plagnes-Juan, Stephane Panserat, and Surintorn Boonanuntanasarn
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Aquatic Science - Published
- 2022
18. MicroRNAs related to cholesterol metabolism affected by vegetable diet in rainbow trout (Oncorhynchus mykiss) from control and selected lines
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Mathilde Dupont-Nivet, Sandrine Skiba-Cassy, Jérôme Montfort, Edwige Quillet, Tengfei Zhu, Geneviève Corraze, Elisabeth Plagnes-Juan, Julien Bobe, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, ANR-13-ADAP-001 AGREENFISH, Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), This work was carried out within the framework of the AGREENFISH project (project ANR-13-ADAP-001) funded by the French National Research Agency (ANR). T. Zhu gratefully acknowledges the financial assistance provided by the China Scholarship Council (CSC, File No. 201406330071) for his doctoral fellowship., and ANR-13-ADAP-0001,AGREENFISH,Adaptations aux transitions alimentaires en aquaculture: les caractériser et les favoriser(2013)
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food intake ,[SDV]Life Sciences [q-bio] ,adaptation ,poisson ,Aquaculture ,salmonids ,lipid metabolism ,Food science ,adaptation alimentaire ,métabolisme du cholestérol ,salmonidae ,trout ,alimentation ,oncorhynchus mykiss ,0303 health sciences ,biology ,04 agricultural and veterinary sciences ,foie ,Fish oil ,rainbow trout ,microRNAs ,régime alimentaire ,Trout ,micro arn ,expression des gènes ,Aquatic Science ,Selective breeding ,03 medical and health sciences ,Fish meal ,rainbow ,transcription factors ,croissance animale ,nutrition animale ,signal de transduction ,14. Life underwater ,Salmonidae ,030304 developmental biology ,fish ,apoptose ,métabolisme lipidique ,business.industry ,Lipid metabolism ,biology.organism_classification ,plant-based diet ,alimentation végétale ,animal nutrition ,cholesterol metabolism ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,Rainbow trout ,liver function tests ,business ,facteur de transcription ,animal growth ,truite arc en ciel - Abstract
For the sustainable development of aquaculture, vegetable ingredients have been used to replace the traditional ingredients fishmeal and fish oil in aquafeeds. On the other hand, selective breeding has also been implemented at research level to obtain strains that are adapted to feeding on the plant-based diet. To better understand the underlying mechanisms prompting the adaptation to plant-based diets in fish, we investigated the hepatic expression of several microRNAs (miRNAs) that are involved in the post-transcriptional regulation of cholesterol and lipid metabolism at 8 h and 16 h after the last meal in two lines of rainbow trout: one selected for better adaptation to plant-based diets and the corresponding control line. Both groups were fed either a fishmeal and fish oil based diet or a 100% plant-based diet. Results showed that the expression of miR-33a in liver was greatly elevated in trout fed the plant-based diet, while the expression of miR-122 and miR-128 was much higher in the selected line at 8 h after the last meal regardless of the diet. Furthermore, our results indicated that some genes involved in immune processes (caspase 6 apoptosis-related cysteine peptidase like 2, casp6l2) and cAMP signal transduction (phosphodiesterase 4B cAMP-specific a, pde4ba) were also potentially regulated by miRNAs. They were newly identified as putative direct targets of miRNAs and affected in trout fed the plant-based diet. Though further investigations are still needed to establish a valid relationship between miRNAs and their target genes, our study found miR-33a, miR-122 and miR-128 as potential candidates for further study and provided new perspectives to understand the role of miRNAs in the selective breeding for adaptation to the plant-based diets.
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- 2019
19. Glucose Injection Into Yolk Positively Modulates Intermediary Metabolism and Growth Performance in Juvenile Nile Tilapia (Oreochromis niloticus)
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Elisabeth Plagnes-Juan, Vincent Veron, Lucie Marandel, Suksan Kumkhong, Surintorn Boonanuntanasarn, Stéphane Panserat, School of Animal Production Technology, Institute of Agricultural Technology, Suranaree University of Technology (SUT), Nutrition, Métabolisme, Aquaculture (NuMéA), Université de Pau et des Pays de l'Adour (UPPA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and This work was supported by Suranaree University of Technology (SUT) and by the Office of the Higher Education Commission under the NRUProject of Thailand. In addition, financial support from the Thailand Research Fund through the Royal Golden Jubilee Ph.D. Program (Grant No. PHD/0138/2557) to student’s initials and adviser’s initials is gratefully acknowledged.
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medicine.medical_specialty ,Physiology ,glucose metabolism ,Carbohydrate metabolism ,Biology ,lcsh:Physiology ,nile tilapia ,03 medical and health sciences ,Nile tilapia ,Physiology (medical) ,Internal medicine ,medicine ,Glycolysis ,Original Research ,030304 developmental biology ,2. Zero hunger ,0303 health sciences ,lcsh:QP1-981 ,Catabolism ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,Glucose transporter ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,04 agricultural and veterinary sciences ,biology.organism_classification ,glucose injection ,Endocrinology ,Gluconeogenesis ,040102 fisheries ,biology.protein ,gene expression ,0401 agriculture, forestry, and fisheries ,nutritional programming ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,Pyruvate kinase ,GLUT4 - Abstract
International audience; The aim of this study was to explore for the first time in omnivorous fish the concept of nutritional programming. A nutritional stimulus was accomplished by microinjecting 2 M glucose into yolk reserves during the alevin stage in Nile tilapia (Oreochromis niloticus). At the molecular level in fry, at 1 week post-injection, glucose stimuli were associated with the up-regulation of genes involved in glycolysis (pklr, hk1, hk2, and pkma), glucose transport (glut4) pathways and down-regulation of genes related to gluconeogenesis (g6pca1, g6pca2, and pck1) and amino acid catabolism (asat, alat) (P < 0.05), demonstrating that the larvae well received the glucose stimulus at a molecular level. Moreover, 20 weeks after glucose injection, early glucose stimuli were always linked to permanent effects in juvenile fish, as reflected by a higher level of glycolytic enzymes [gck, hk1 and hk2 at both mRNA and enzymatic levels and pyruvate kinase (PK) activity]. Finally, the effects of the glucose stimulus history were also examined in fish fed with two different dietary carbohydrate/protein levels (medium-carbohydrate diet, CHO-M; high-carbohydrate diet, CHO-H) in juvenile fish (during weeks 20-24). As expected, the CHO-H diet induced the expression of glycolytic and lipogenic genes (gck, pklr, hk1, hk2, fpkma, fasn, and g6pd) and suppressed the expression of gluconeogenic and amino acid catabolism genes (g6pca1, pck1, pck2, asat, alat, and gdh). Nevertheless, the early glucose stimulus led to persistent up-regulation of glycolytic enzymes (gck, pklr, hk1, and hk2) at both the mRNA and enzyme activity levels and glucose transporter glut4 as well as lower gluconeogenic pck1 gene expression (P < 0.05). More interestingly, the early glucose stimulus was associated with a better growth performance of juvenile fish irrespective of the diets. These permanent changes were associated with DNA hypomethylation in the liver and muscles, suggesting the existence of epigenetic mechanisms at the origin of programming. In conclusion, for the first time in tilapia, early glucose stimuli were found to be clearly associated with a positive metabolic programming effect later in life, improving the growth performance of the fish.
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- 2020
20. Early feeding with hyperglucidic diet during fry stage exerts long-term positive effects on nutrient metabolism and growth performance in adult tilapia ( Oreochromis niloticus )
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Lucie Marandel, Surintorn Boonanuntanasarn, Vincent Veron, Suksan Kumkhong, Elisabeth Plagnes-Juan, Stéphane Panserat, School of Animal Technology and Innovation, Nutrition, Métabolisme, Aquaculture (NuMéA), Université de Pau et des Pays de l'Adour (UPPA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Suranaree University of Technology, Office of the Higher Education Commission under the NRU Project of Thailand, Thailand Research Fund through the Royal Golden Jubilee Ph.D. Program (Grant No. PHD/0138/2557), French Government (N° de dossier Campus France: 931061D), and Embassy of France in Thailand to the RGJ-Ph.D. program
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0301 basic medicine ,medicine.medical_specialty ,food.ingredient ,Growth performance ,Endocrinology, Diabetes and Metabolism ,Carbohydrate metabolism ,03 medical and health sciences ,Nile tilapia ,chemistry.chemical_compound ,food ,Rivers ,Internal medicine ,Diet, Protein-Restricted ,Dietary Carbohydrates ,medicine ,Animals ,14. Life underwater ,2. Zero hunger ,Glucose metabolism ,Nutrition and Dietetics ,biology ,Glycogen ,Catabolism ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,Gluconeogenesis ,Tilapia ,04 agricultural and veterinary sciences ,Metabolism ,biology.organism_classification ,Animal Feed ,Diet ,Oreochromis ,030104 developmental biology ,Endocrinology ,chemistry ,Lipogenesis ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,Egypt ,nutrient programming ,early feeding ,Gene expression ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,Research Article ,Food Science - Abstract
The present study aimed to investigate nutritional programming of carbohydrate metabolism in Nile tilapia. Early nutritional intervention stimulus was achieved by feeding fry with high-protein/low-carbohydrate (HP/LC) or low-protein/high-carbohydrate (LP/HC) diet since first feeding for 4 weeks, and the effect of nutritional stimulus on carbohydrate and its related metabolism was evaluated through the adult stage. Our findings indicated that at week 1, LP/HC diet-fed fry had lower levels of mRNA for genes coding gluconeogenesis and amino acid catabolism and higher levels ofhk2 (P< 0⋅05). As expected, in adult tilapia, although LP/HC diet-fed fish had poorer growth (end of stimulus), the fish showed compensatory growth. There were permanent effects of early high-carbohydrate (HC) intake on several parameters, including (1) modulating hepatic composition, (2) increased muscle glycogen, (3) lower levels of enzymes involved in amino acid catabolism and (4) higher levels of glycolytic enzymes in glycolysis. Finally, HP/LC diet- and LP/HC diet-fed fish were challenged with different dietary carbohydrate levels. Irrespective of challenging diets, the early HC stimulus had significant effects on adult tilapia by (1) promoting utilisation of glucose, which had protein-sparing effects for better growth, (2) inducting lipogenesis and (3) decreasing amino acid catabolism. Taken together, for the first time, we demonstrated that early HC feeding was effective for positive nutritional programming of metabolism in Nile tilapia (an omnivorous fish). It led to the improvement of growth performance in adult fish associated with early feeding, which is linked to a better ability to use glucose, to induce lipogenesis, and to suppress amino acid catabolism.
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- 2020
21. Cholesterol metabolism regulation mediated by SREBP-2, LXRα and miR-33a in rainbow trout (Oncorhynchus mykiss) both in vivo and in vitro
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Geneviève Corraze, Elisabeth Plagnes-Juan, Tengfei Zhu, Sandrine Skiba-Cassy, Nutrition, Métabolisme, Aquaculture (NuMéA), Université de Pau et des Pays de l'Adour (UPPA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and China Scholarship Council (CSC, File No. 201406330071)
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0301 basic medicine ,Trout ,Gene Expression ,Aquaculture ,030204 cardiovascular system & hematology ,Biochemistry ,Receptors, G-Protein-Coupled ,chemistry.chemical_compound ,0302 clinical medicine ,Animal Cells ,Gene expression ,Transcriptional regulation ,Medicine and Health Sciences ,Cells, Cultured ,Liver X Receptors ,Mammals ,Multidisciplinary ,biology ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,Eukaryota ,Lipids ,Nucleic acids ,Cholesterol ,Liver ,Osteichthyes ,Oncorhynchus mykiss ,Vertebrates ,Medicine ,lipids (amino acids, peptides, and proteins) ,Cellular Types ,Anatomy ,Research Article ,medicine.medical_specialty ,Fish Biology ,Science ,03 medical and health sciences ,In vivo ,Internal medicine ,medicine ,Genetics ,Fish Physiology ,Animals ,Animal Physiology ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,14. Life underwater ,Liver X receptor ,Non-coding RNA ,Nutrition ,Natural antisense transcripts ,Organisms ,Biology and Life Sciences ,Cell Biology ,biology.organism_classification ,Lipid Metabolism ,Sterol ,Vertebrate Physiology ,Sterol regulatory element-binding protein ,Diet ,Gene regulation ,MicroRNAs ,030104 developmental biology ,Endocrinology ,Fish ,chemistry ,Amniotes ,Hepatocytes ,RNA ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,Zoology - Abstract
International audience; Cholesterol metabolism is greatly affected in fish fed plant-based diet. The regulation of cholesterol metabolism is mediated by both transcriptional factors such as sterol regulatory element binding proteins (SREBPs) and liver X receptors (LXRs), and posttranscriptional factors including miRNAs. In mammals, SREBP-2 and LXRα are involved in the transcrip-tional regulation of cholesterol synthesis and elimination, respectively. In mammals, miR-33a is reported to directly target genes involved in cholesterol catabolism. The present study aims to investigate the regulation of cholesterol metabolism by SREBP-2 and LXRα and miR-33a in rainbow trout using in vivo and in vitro approaches. In vivo, juvenile rainbow trout of~72 g initial body weight were fed a total plant-based diet (V) or a marine diet (M) containing fishmeal and fish oil. In vitro, primary cell culture hepatocytes were stimulated by graded concentrations of 25-hydroxycholesterol (25-HC). The hepatic expression of cholesterol synthetic genes, srebp-2 and miR-33a as well as miR-33a level in plasma were increased in fish fed the plant-based diet, reversely, their expression in hepatocytes were inhibited with the increasing 25-HC in vitro. However, lxrα was not affected neither in vivo nor in vitro. Our results suggest that SREBP-2 and miR-33a synergistically enhance the expression of cholesterol synthetic genes but do not support the involvement of LXRα in the regulation of cholesterol elimination. As plasma level of miR-33a appears as potential indicator of cholesterol synthetic capacities, this study also highlights circulating miRNAs as promising noninvasive biomarker in aquaculture.
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- 2020
22. Composition of Intestinal Microbiota in Two Lines of Rainbow Trout (Oncorhynchus Mykiss) Divergently Selected for Muscle Fat Content
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Stéphane Panserat, Sandrine Skiba-Cassy, Maxime Even, Laurence Larroquet, Mickael Rey, Elisabeth Plagnes-Juan, Edwige Quillet, Karine Ricaud, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, and UPPA / Conseil Départemental des Landes
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0301 basic medicine ,rainbow trout ,gut microbiota ,selection ,fish line ,adiposity ,aquaculture ,endocrine system ,animal structures ,Firmicutes ,animal diseases ,030106 microbiology ,Zoology ,Gut microbiota ,Aquaculture ,Gut flora ,digestive system ,Microbiology ,Actinobacteria ,03 medical and health sciences ,microbiote ,Biologie animale ,14. Life underwater ,Selection ,ComputingMilieux_MISCELLANEOUS ,Adiposity ,Animal biology ,oncorhynchus mykiss ,General Immunology and Microbiology ,biology ,urogenital system ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,Hindgut ,Midgut ,Lipid metabolism ,biology.organism_classification ,Fish lines ,adiposité ,030104 developmental biology ,Rainbow trout ,sélection animale ,Proteobacteria - Abstract
Background:Recently, studies suggest that gut microbiota contributes to the development of obesity in mammals. In rainbow trout, little is known about the role of intestinal microbiota in host physiology.Objective:The aim of this study was to investigate the link between intestinal microbiota and adiposity, by high-throughput 16S RNA gene based illumina Miseq sequencing in two rainbow trout lines divergently selected for muscle lipid content. Fish from these two lines of rainbow trout are known to have a differing lipid metabolism.Methods:Samples from the two lines (L for lean and F for fat) were collected from Midgut (M) and Hindgut (H) in juvenile fish (18 months) to compare intestinal microbiota diversity.Results:Whatever the lines and intestinal localisation,Proteobacteria,FirmicutesandActinobacteriaare the dominant phyla in the bacterial community of rainbow trout (at least 97%). The results indicate that richness and diversity indexes as well as bacterial composition are comparable between all groups even though 6 specific OTUs were identified in the intestinal microbiota of fish from the fat line and 2 OTUs were specific to the microbiota of fish from the lean line. Our work contributes to a better understanding in microbial diversity in intestinal microbiota of rainbow trout.Conclusion:Altogether, our study indicates that no major modification of the intestinal microbiota is induced by selection for muscle lipid content and associated metabolic changes. Finally, we identified members of core microbiota in rainbow trout.
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- 2018
23. Ontogenesis of metabolic gene expression in whiteleg shrimp (Litopenaeus vannamei): New molecular tools for programming in the future
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Luis Paulo Araujo Lage, Stéphane Panserat, Alberto J.P. Nunes, Elisabeth Plagnes-Juan, Alain Guyonvarch, Delphine Weissman, Soraia Marques Putrino, Frederic Baron, Rolland Brugger, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), LABOMAR Instituto de Ciencias do Mar, Universidade Federal do Ceará (UFC), NEOVIA InVivo, ANRT and CNPq for funding Luis Paulo Araujo Lage (CIFRE-Brasil/CNPq - GDE – France/Number 217420/2014-1, and Universidade Federal do Ceará = Federal University of Ceará (UFC)
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0301 basic medicine ,Ontogeny ,Litopenaeus ,digestion ,Aquatic Science ,programming ,03 medical and health sciences ,Gene expression ,Whiteleg shrimp ,crevette ,nutrition animale ,métabolisme ,Gene ,biology ,Ecology ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,ontogenesis ,Lipid metabolism ,programmation nutritionnelle ,04 agricultural and veterinary sciences ,Metabolism ,biology.organism_classification ,Shrimp ,ontogénèse ,030104 developmental biology ,aquaculture ,Biochemistry ,white shrimp ,animal nutrition ,gene expression ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,shrimp ,metabolism ,expression des gènes - Abstract
Nutritional programming is a very promising strategy for modifying nutrient metabolism to better adapt animals to new diets. Although it is a well-known approach in fish-farmed species, it has never been applied in marine shrimp. A preliminary requirement to perform nutritional programming is to identify the best developmental window for early stimulus. The objective of the present study was to characterize the ontogenesis of the expression of the main genes involved in digestion and metabolism in white shrimp, Litopenaeus vannamei. Four genes involved in digestion, 11 genes in intermediary metabolism (glucose, amino acid and lipid metabolism) and 5 genes in energy metabolism (mitochondria) were studied at 10 different developmental stages (from eggs – 8 h after fertilization - up to post-larval substage 5) using qRT-PCR for measuring mRNA levels. Our data show that almost all of the studied genes present higher mRNA levels during the protozoea substage Z1, which could be explained by the endogenous-exogenous feeding transition in white shrimp. In conclusion, Z1 substage shows a high molecular plasticity for metabolism which becomes the preferential developmental window for future programming experiments in L. vannamei. Statement of relevance Our study aimed at characterizing for the first time the ontogenesis of expressions of genes involved in intermediary metabolism (nutrient metabolism). This approach determined developmental windows with high molecular plasticity (Z1 and PL stages). These data will support future studies in nutritional programming for the whiteleg shrimp by determining the timing for early stimulus.
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- 2017
24. Evolutionary history of glucose-6-phosphatase encoding genes in vertebrate lineages: towards a better understanding of the functions of multiple duplicates
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Elisabeth Plagnes-Juan, Eva Arbenoits, Stéphane Panserat, Lucie Marandel, José L. Soengas, Julien Bobe, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Departamento de Bioloxia Funcional e Ciencias da Saude, Laboratorio de Fisioloxia Animal, Facultade de Bioloxia, Universidate de Vigo, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), This study was supported by research grants from the INRA PHASE Department (France) to LM and from the Ministerio de Economía y Competitividad (Spain) and European Fund for Regional Development to JLS (AGL2016-74857-C3-1-R and FEDER)., Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), and Universidade de Vigo
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0301 basic medicine ,Sarcopterygii ,glucose 6 phosphate ,Duplicated genes ,Proteomics ,Teleosts ,chemistry.chemical_compound ,0302 clinical medicine ,poisson ,duplication de gènes ,Gene duplication ,Glucose homeostasis ,glucose ,duplication des génomes ,Phylogeny ,ComputingMilieux_MISCELLANEOUS ,Animal biology ,Genetics ,trout ,teleost ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,Vertebrate ,Heart ,duplicated gene ,Phenotype ,Vertebrates ,Glucose-6-Phosphatase ,teleosteen ,DNA microarray ,duplicate genes ,expression des gènes ,Research Article ,Biotechnology ,lcsh:QH426-470 ,brain ,lcsh:Biotechnology ,truite ,Biology ,analyse phylogénétique ,Synteny ,Gene Expression Regulation, Enzymologic ,Evolution, Molecular ,03 medical and health sciences ,biology.animal ,lcsh:TP248.13-248.65 ,Biologie animale ,Dietary Carbohydrates ,Animals ,Humans ,gène dupliqué ,Gene ,Actinopterygii ,Myocardium ,sarcopterygii ,actinopterygii ,vertébré ,lcsh:Genetics ,030104 developmental biology ,Glucose 6-phosphate ,chemistry ,Evolutionary biology ,030217 neurology & neurosurgery ,homéostasie glucidique - Abstract
Background Glucose-6-phosphate (G6pc) is a key enzyme involved in the regulation of the glucose homeostasis. The present study aims at revisiting and clarifying the evolutionary history of g6pc genes in vertebrates. Results g6pc duplications happened by successive rounds of whole genome duplication that occurred during vertebrate evolution. g6pc duplicated before or around Osteichthyes/Chondrichthyes radiation, giving rise to g6pca and g6pcb as a consequence of the second vertebrate whole genome duplication. g6pca was lost after this duplication in Sarcopterygii whereas both g6pca and g6pcb then duplicated as a consequence of the teleost-specific whole genome duplication. One g6pca duplicate was lost after this duplication in teleosts. Similarly one g6pcb2 duplicate was lost at least in the ancestor of percomorpha. The analysis of the evolution of spatial expression patterns of g6pc genes in vertebrates showed that all g6pc were mainly expressed in intestine and liver whereas teleost-specific g6pcb2 genes were mainly and surprisingly expressed in brain and heart. g6pcb2b, one gene previously hypothesised to be involved in the glucose intolerant phenotype in trout, was unexpectedly up-regulated (as it was in liver) by carbohydrates in trout telencephalon without showing significant changes in other brain regions. This up-regulation is in striking contrast with expected glucosensing mechanisms suggesting that its positive response to glucose relates to specific unknown processes in this brain area. Conclusions Our results suggested that the fixation and the divergence of g6pc duplicated genes during vertebrates’ evolution may lead to adaptive novelty and probably to the emergence of novel phenotypes related to glucose homeostasis. Electronic supplementary material The online version of this article (doi:10.1186/s12864-017-3727-1) contains supplementary material, which is available to authorized users.
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- 2017
25. Eating for two: Consequences of parental methionine nutrition on offspring metabolism in rainbow trout (Oncorhynchus mykiss)
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Lucie Marandel, Esmail Lutfi, Sandrine Skiba-Cassy, Inge Geurden, Karine Dias, Iban Seiliez, Emilio J. Vélez, Elisabeth Plagnes-Juan, Stéphane Panserat, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Departament de Biologia Cellular, Fisiologia i Immunologia, Facultat de Biologia, Universitat Autònoma de Barcelona (UAB), This study was supported by the European Union Seventh Framework Programme (Project Call Identifier: FP7-KBBE-2011-5, Project Number: 288925, Advanced Research Initiatives for Nutrition & Aquaculture (ARRAINA)). E.J.V. and E.L. are supported by a predoctoral fellowship from the Ministerio de Ciencia e Innovación (MICINN), Spain, European Project: 288925, and Universitat Autònoma de Barcelona [Barcelona] (UAB)
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0301 basic medicine ,medicine.medical_specialty ,Offspring ,[SDV]Life Sciences [q-bio] ,reproducteur ,Broodstock ,Aquatic Science ,Biology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Sequestosome 1 ,broodstock ,poisson ,Internal medicine ,medicine ,14. Life underwater ,education ,métabolisme ,ComputingMilieux_MISCELLANEOUS ,fish ,fry ,oncorhynchus mykiss ,education.field_of_study ,Methionine ,méthionine ,programmation nutritionnelle ,Methylation ,Metabolism ,rainbow trout ,nutrition ,030104 developmental biology ,Endocrinology ,chemistry ,Gluconeogenesis ,Rainbow trout ,metabolism ,nutritional programing ,030217 neurology & neurosurgery - Abstract
International audience; Parental nutrition events may result in permanent changes in the growth potential, health and metabolic status of the next generation. In the present study,we aimed to determine in rainbow trout (Oncorhynchusmykiss), the effect of feeding broodstock with a diet deficient in methionine (one of the main methyl-group donor needed for all biological methylation reactions including DNA and histones methylation) on the response of several metabolism- and growth-related genes and proteins in offspring subjected to different dietary methionine levels (deficiency or adequate). Adult males and females were fed for 6 months with either a methionine deficient diet or a control diet. Males and females of the same feeding group were then crossed and the obtained fry were fed with either a methionine deficient or a control diet for 3 weeks from the first exogenous feeding. The obtained results indicated that, whatever the methionine content of the broodstock diet, a 3weeks dietary methionine deficiency in fry strongly affected the levels of transcripts and/or proteins involved in fatty acid synthesis, cholesterol synthesis and the two main cell quality control mechanisms, namely the ubiquitin-proteasome system and autophagy. In addition, our data also demonstrated that the levels of some of these mRNA and/or proteins are also affected by the dietary methionine deficiency in broodstock. Of interest, some genes (involved in gluconeogenesis and autophagy) were only affected by the parental and not by the fry diet. Together, these results clearly show that the broodstock diet may influence the metabolism of the progeny in rainbow trout and highlight the potential to apply new feeding strategies adapted to plant feedstuffs
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- 2017
26. Looking at the complex relationships between migration behavior and conditional strategy based on energy metabolism in the European glass eel (Anguilla anguilla)
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Elisabeth Plagnes-Juan, Pascale Coste, Jacques Rives, Emmanuel Huchet, Iban Seiliez, Vincent Veron, Valérie Bolliet, Hengtong Liu, Jacques Labonne, Ecologie Comportementale et Biologie des Populations de Poissons (ECOBIOP), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Collège STEE Côte Basque, E2S, MIRA, Université de Pau et des Pays de l'Adour (UPPA), and Nutrition, Métabolisme, Aquaculture (NuMéA)
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autophagy ,animal structures ,Environmental Engineering ,010504 meteorology & atmospheric sciences ,métabolisme énergétique ,Energy metabolism ,Zoology ,autophagie ,010501 environmental sciences ,migration ,01 natural sciences ,Anguillidae ,Environmental Chemistry ,anguilla anguilla ,14. Life underwater ,Waste Management and Disposal ,ComputingMilieux_MISCELLANEOUS ,0105 earth and related environmental sciences ,Facultative ,geography ,geography.geographical_feature_category ,biology ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,Energetics ,glass eel ,comportement migratoire ,Estuary ,biology.organism_classification ,Stress resistance ,Pollution ,migration behavior ,Spatial dispersion ,Metabolic rate ,energetic status ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,metabolism ,anguillidae - Abstract
International audience; Diadromy in eel is facultative and the diverse propensities in glass eel estuarine migration may lead to a large spatial dispersion, which may has profound influences on the species sex determination. In this study, we sought to clarify the relationship between European glass eel energetics and their pattern of migration behavior, in the framework of the conditional strategy. Marine glass eels were sampled in autumn and spring, stratifying high and low energetic status, respectively. Their migration behavior was determined in experimental installations that mimic tides using a change in water current direction every 6.2 h. When synchronized to the current reversal, glass eels were called active while individuals hiding in the substratum were considered as non-active. Then, for each active fish, a level of swimming activity was determined and both migration behavior and level of swimming activity were correlated to the individual wet weight (used as a proxy of energy stores), standard metabolic rate (relative SMR) and transcriptomic profile of metabolism related genes. Results showed that spring glass eels presented a lower probability to migrate, a lower wet weight and a higher expression of genes involved in energy stress resistance than autumn ones, supporting a conditional strategy based on individual's energy status. However, within each season, no wet weight difference was observed between active and non-active fish. In autumn glass eels, migration behavior was weakly related to relative SMR while in spring, none of the parameters analyzed allows discriminating active and non-active glass eels. The level of swimming activity in active fish was related to their relative SMR in autumn and to their wet weight in spring. Altogether, our results could not validate a conditional strategy but spring glass eels displayed some signs of energy distress and a lower level of swimming activity than autumn ones
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- 2019
27. Utilisation du glucose en environnement austral par des salmonidés carnivores
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Lucie Marandel, Philippe Gaudin, Francois Gueraud, Stéphane Glise, Alexandre Herman, Elisabeth Plagnes-Juan, Vincent Véron, Stéphane Panserat, Jacques Labonne, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), and Ecologie Comportementale et Biologie des Populations de Poissons (ECOBIOP)
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[SDV.BA]Life Sciences [q-bio]/Animal biology ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2018
28. Circulating miRNA measurements are reflective of cholesterol-based changes in rainbow trout (Oncorhynchus mykiss)
- Author
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Elisabeth Plagnes-Juan, Tengfei Zhu, Geneviève Corraze, Sandrine Skiba-Cassy, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), and T. Zhu gratefully acknowledges the financial assistance provided by the China Scholarship Council (CSC, File No. 201406330071) for his doctoral fellowship.
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0301 basic medicine ,Circulating mirnas ,Blood Glucose ,Physiology ,lcsh:Medicine ,Biochemistry ,chemistry.chemical_compound ,Medicine and Health Sciences ,lcsh:Science ,métabolisme du cholestérol ,ComputingMilieux_MISCELLANEOUS ,Animal biology ,trout ,oncorhynchus mykiss ,Multidisciplinary ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,Eukaryota ,cholestérol ,Postprandial Period ,Lipids ,rainbow trout ,Body Fluids ,microRNAs ,Nucleic acids ,Postprandial ,Cholesterol ,Blood ,Liver ,Osteichthyes ,Vertebrates ,micro arn ,Efflux ,Anatomy ,Research Article ,expression des gènes ,medicine.medical_specialty ,Fish Biology ,Biology ,Blood Plasma ,03 medical and health sciences ,rainbow ,Internal medicine ,microRNA ,Biologie animale ,medicine ,Genetics ,Fish Physiology ,Animals ,Animal Physiology ,14. Life underwater ,Non-coding RNA ,Gene ,Triglycerides ,Natural antisense transcripts ,Biology and life sciences ,Gene Expression Profiling ,Lipogenesis ,lcsh:R ,Organisms ,Lipid Metabolism ,Vertebrate Physiology ,Gene regulation ,Gene expression profiling ,030104 developmental biology ,Endocrinology ,Fish ,chemistry ,RNA ,Rainbow trout ,lcsh:Q ,Gene expression ,Zoology ,Biomarkers ,truite arc en ciel - Abstract
International audience; MicroRNAs (miRNAs) are a class of small non-coding RNAs which are known to posttranscriptionally regulate the expression of most genes in both animals and plants. Meanwhile, studies have shown that numbers of miRNAs are present in body fluids including the plasma. Despite the mode of action of these circulating miRNAs still remains unknown, they have been found to be promising biomarkers for disease diagnosis, prognosis and response to treatment. In order to evaluate the potential of miRNAs as non-invasive biomarkers in aquaculture, a time-course experiment was implemented to investigate the postprandial regulation of miRNAs levels in liver and plasma as well as the hepatic expression of genes involved in cholesterol metabolism. We showed that miR-1, miR-33a, miR-122, miR-128 and miR-223 were expressed in the liver of rainbow trout and present at detectable level in the plasma. We also demonstrated that hepatic expression of miR-1, miR-122 and miR-128 were regulated by feed intake and reached their highest levels 12 hours after the meal. Interestingly, we observed that circulating levels of miR-128 and miR-223 are subjected to postprandial regulations similar to that observed in their hepatic counterparts. Statistical correlations were observed between liver and plasma for miR-128 and miR-223 and between hepatic and circulating miR-122, miR-128 and miR-223 and expression of genes related to cholesterol synthesis and efflux or glucose phosphorylation. These results demonstrated that circulating miR-122, miR-128 and miR-223 are potential biomarkers of cholesterol metabolism in rainbow trout.
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- 2018
29. Regulation of genes related to cholesterol metabolism in rainbow trout (Oncorhynchus mykiss) fed a plant-based diet
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Tengfei Zhu, Edwige Quillet, Elisabeth Plagnes-Juan, Geneviève Corraze, Sandrine Skiba-Cassy, Mathilde Dupont-Nivet, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, and AgroParisTech-Institut National de la Recherche Agronomique (INRA)
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0301 basic medicine ,Physiology ,chemistry.chemical_compound ,Homeostasis ,métabolisme du cholestérol ,2. Zero hunger ,chemistry.chemical_classification ,oncorhynchus mykiss ,biology ,microRNA ,Diet, Vegetarian ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,cholestérol ,04 agricultural and veterinary sciences ,Fish oil ,Adaptation, Physiological ,rainbow trout ,Trout ,Cholesterol ,Animal Nutritional Physiological Phenomena ,lipids (amino acids, peptides, and proteins) ,animal feeding ,expression des gènes ,Fish Proteins ,medicine.medical_specialty ,alimentation animale ,Nutritional Status ,Cholesterol 7 alpha-hydroxylase ,digestive system ,Gene Expression Regulation, Enzymologic ,Bile Acids and Salts ,03 medical and health sciences ,Fish meal ,Physiology (medical) ,Internal medicine ,medicine ,Animals ,lxr ,nutrition animale ,fish ,aliment à base de plante ,Fatty acid ,Lipid metabolism ,biology.organism_classification ,Lipid Metabolism ,Animal Feed ,MicroRNAs ,030104 developmental biology ,Endocrinology ,chemistry ,plant-based diet ,srebp-2 ,animal nutrition ,040102 fisheries ,cholesterol metabolism ,0401 agriculture, forestry, and fisheries ,Rainbow trout - Abstract
When compared with fish meal and fish oil, plant ingredients differ not only in their protein content and amino acid and fatty acid profiles but are also devoid of cholesterol, the major component of cell membrane and precursor of several bioactive compounds. Based on these nutritional characteristics, plant-based diets can affect fish physiology and cholesterol metabolism. To investigate the mechanisms underlying cholesterol homeostasis, rainbow trout were fed from 1 g body wt for 6 mo with a totally plant-based diet (V), a marine diet (M), and a marine-restricted diet (MR), with feed intake adjusted to that of the V group. The expression of genes involved in cholesterol synthesis, esterification, excretion, bile acid synthesis, and cholesterol efflux was measured in liver. Results showed that genes involved in cholesterol synthesis were upregulated in trout fed the V diet, whereas expression of genes related to bile acid synthesis ( cyp7a1) and cholesterol elimination ( abcg8) were reduced. Feeding trout the V diet also enhanced the expression of srebp-2 while reducing that of lxrα and miR-223. Overall, these data suggested that rainbow trout coped with the altered nutritional characteristics and absence of dietary cholesterol supply by increasing cholesterol synthesis and limiting cholesterol efflux through molecular mechanisms involving at least srebp-2, lxrα, and miR-223. However, plasma and body cholesterol levels in trout fed the V diet were lower than in fish fed the M diet, raising the question of the role of cholesterol in the negative effect of plant-based diet on growth.
- Published
- 2018
30. Adaptation of Nile tilapia ( Oreochromis niloticus ) to different levels of dietary carbohydrates: New insights from a long term nutritional study
- Author
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Suksan Kumkhong, Araya Jangprai, Christine Burel, Vincent Veron, Lucie Marandel, Stéphane Panserat, Elisabeth Plagnes-Juan, Surintorn Boonanuntanasarn, Institute of Agricultural Technology, Suranaree University of Technology, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), We acknowledge the project France Campus Hubert Curien PHC Siam n° 35445ZC (2016-2017) for funding the research 'Molecular and biochemical adaptation of Nile Tilapia to dietary carbohydrates' carried out between NuMeA INRA laboratory and Suranaree University of Technology. This work was supported by Suranaree University of Technology (SUT) and by the Office of the Higher Education Commission under the NRU project of Thailand, and Suranaree University of Technology (SUT)
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0301 basic medicine ,tilapia ,glucose metabolism ,Aquatic Science ,Carbohydrate metabolism ,nile tilapia ,03 medical and health sciences ,Nile tilapia ,14. Life underwater ,Food science ,nutrition animale ,métabolisme du glucose ,ComputingMilieux_MISCELLANEOUS ,2. Zero hunger ,biology ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,04 agricultural and veterinary sciences ,biology.organism_classification ,dextrine ,Oreochromis ,030104 developmental biology ,Gluconeogenesis ,phosphodextrins ,animal nutrition ,Lipogenesis ,040102 fisheries ,gene expression ,0401 agriculture, forestry, and fisheries ,Phosphoenolpyruvate carboxykinase ,dextrin ,Pyruvate kinase ,Dietary Carbohydrates ,expression des gènes - Abstract
International audience; The objective of this study was to examine the long-term effects of different levels of dietary carbohydrates on glucose metabolism in the omnivorous Nile Tilapia (Oreochromis niloticus). Fish were fed from the first feeding stage until the adult stage, over a period of 40 weeks, using three different levels of carbohydrates (dextrin): 0% (CHO-L), 30% (CHO-M) and 50% (CHO-H). Growth performance, blood metabolite parameters, the proximate composition of the whole body, muscle and liver tissue, and the mRNA levels for genes involved in glycolysis, gluconeogenesis, lipogenesis and glucose transport in liver and muscle tissue were analyzed at week 26 and week 40. Fish fed the CHO-M diet exhibited the best growth performance, while the fish fed with the CHO-H diet showed the lowest growth performance. These data suggest that the incorporation of a certain level dietary carbohydrates can benefit Nile Tilapia. However, if the proportion of carbohydrates is very high, and concomitantly, the level of dietary proteins too low, this will not promote growth. Fish fed with carbohydrates exhibited a significant increase in lipid deposition in the whole body, muscle and liver tissues in. This seems to be due to increased lipogenic capacity, as reflected by higher hepatic fatty acid synthase mRNA levels and activity, as well as higher levels of plasma triglyceride. Nile Tilapia appear to be able to effectively adapt to the intake of carbohydrates, as reflected by: (i) the weak postprandial hyperglycemia (5.3–6.1 mM), (ii) the higher level of hepatic glycogen, (iii) the higher glycolytic pyruvate kinase enzyme in muscle (mRNA level and activity) and (iv) the inhibition of the gluconeogenic pathway for glucose-6-phosphatase and phosphoenolpyruvate carboxykinase enzymes (at molecular and enzymatic levels). Overall, these data show that the well-known differences in the capacity of carnivorous and omnivorous fish species to use dietary carbohydrates could be linked to differences in the regulation of the gluconeogenic and lipogenic pathways.
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- 2018
31. Molecular responses of Nile tilapia ( Oreochromis niloticus ) to different levels of dietary carbohydrates
- Author
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Stéphane Panserat, Suksan Kumkhong, Christine Burel, Kirana Yoohat, Surintorn Boonanuntanasarn, Lucie Marandel, Elisabeth Plagnes-Juan, School of Animal Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), We acknowledge the project France Campus Hubert Curien PHC Siam n° 35445ZC (2016-2017) for the funding of the research 'Molecular and biochemical adaptation of Nile Tilapia to dietary carbohydrates' between NuMeA INRA Laboratory and the University of Surananree Technology University. This work was supported by Suranaree University of Technology (SUT) and by the Office of the Higher Education Commission under NRU project of Thailand, and Suranaree University of Technology (SUT)
- Subjects
0301 basic medicine ,food.ingredient ,tilapia ,alimentation animale ,glucose metabolism ,Aquatic Science ,Biology ,Carbohydrate metabolism ,nile tilapia ,03 medical and health sciences ,Nile tilapia ,chemistry.chemical_compound ,food ,Glucose homeostasis ,14. Life underwater ,métabolisme du glucose ,2. Zero hunger ,Glycogen ,oreochromis niloticus ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,Glucose transporter ,dietary dextrin ,Tilapia ,04 agricultural and veterinary sciences ,biology.organism_classification ,dextrine ,030104 developmental biology ,Biochemistry ,chemistry ,aquaculture ,phosphodextrins ,Lipogenesis ,040102 fisheries ,gene expression ,0401 agriculture, forestry, and fisheries ,animal feeding ,Dietary Carbohydrates ,expression des gènes - Abstract
The objective of the study was to test for the first time the molecular adaptation of the glucose metabolism to this fish species know to be a good user of carbohydrates. In this way, Nile tilapia were fed with 3 different levels of carbohydrates 0% (CHO-L) 30% (CHO-M) and 50% (CHO-H) dextrin. After 45 days and 90 days of feeding we analyzed the plasma parameters, zootechnical performance and the mRNA levels for genes involved in glycolysis, gluconeogenesis, lipogenesis and glucose transport in liver and muscle. The best growth performance was found for fish fed M-CHO diet, the fish fed with the H-CHO diets showing the worst growth performance. Increased of hepatic and muscle glycogen, hepatic somatic index and plasma metabolites (glucose, triglycerides and cholesterol) was linked to the increased of dietary carbohydrates. However, no hyperglycemia, no change of body compositions was found showing that dietary carbohydrates are efficiently used as an energy source in tilapia. Moreover, in contrats to the dietary protein-linked decreased of amino acid catabolic mRNA levels, no clear molecular adaptation for glycolysis, gluconeogenesis, lipogenesis in liver and glycolysis in muscle was detected except higher mRNA levels for glucose transporter in muscle. Our data suggest that these metabolic pathways at a molecular level are not the main actors explaining the efficient use of glucose in tilapia. Statement of relevance Our study aimed at characterizing for the first time the molecular effects of increased dietary carbohydrates (dextrin) from 0% up to 50% in Nile tilapia during 45 and 90 days. Our study confirmed that Nile tilapia can use high level of carbohydrates without any deregulation of glucose homeostasis. However, no strong regulations of expression for genes involved in glucose metabolism in liver and muscle - which could explain the reasons for an efficient use of carbohydrate by this fish species - were detected.
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- 2018
32. Programming of the Glucose Metabolism in Rainbow Trout Juveniles after Chronic Hypoxia at Hatching Stage Combined with a High Dietary Carbohydrate: Protein Ratios Intake at First-Feeding
- Author
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Isabelle Leguen, Lucie Marandel, Stéphane Panserat, Huihua Hu, Elisabeth Plagnes-Juan, Alexandre Herman, Jingwei Liu, Lionel Goardon, Inge Geurden, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Pisciculture Expérimentale INRA des Monts d'Arrée (PEIMA), Institut National de la Recherche Agronomique (INRA), 652831, European Union's Horizon 2020 research and innovation programme, European Project: 652831,H2020,H2020-INFRAIA-2014-2015,AQUAEXCEL2020(2015), and Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA)
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0301 basic medicine ,medicine.medical_specialty ,biology ,Hatching ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,Metabolism ,Aquatic Science ,Carbohydrate metabolism ,Stimulus (physiology) ,Carbohydrate ,biology.organism_classification ,03 medical and health sciences ,Trout ,No keyword ,030104 developmental biology ,Endocrinology ,Internal medicine ,medicine ,Juvenile ,Rainbow trout ,14. Life underwater - Abstract
International audience; It is well known that change in environmental parameters experienced during early life can have profound effect on the metabolism of individuals later in life. The purpose of the present study was to evaluate the impact of a chronic hypoxic stimulus (15 days, 50% dissolved O2, 5.0 mg·L-1) applied at hatching (oxygen history) alone or combined with a 60% high carbohydrate (HC) dietary stimulus (8 days) applied at first-feeding (dietary history) on growth performance and glucose metabolism of juvenile trout. The effectiveness of the hypoxic and the HC dietary stimuli were verified by monitoring the modulation in mRNA levels of oxygen-sensitive and dietary carbohydrate-sensitive genes, respectively. At juvenile stage (28 weeks after first-feeding), neither dietary history nor oxygen history had long-term effect on growth performance. These juveniles were then subjected to a 5 days challenge test with a 30% carbohydrate diet. Our data showed that mRNA levels of some glucose transport-related genes in liver and muscle, and plasma triglycerides were down-regulated in fish which were exposed to hypoxia during early life. Regarding to the effect of dietary history, the mRNA level of muscular pfkmaa was observably increased in fish previously subjected to the HC dietary stimulus. In summary, the present study indicated that a chronic hypoxic stimulus applied at hatching alone or combined with a high carbohydrate stimulus at first-feeding can affect plasma triglycerides level and glucose metabolism-related genes in juvenile trout.
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- 2018
33. A reassessment of the carnivorous status of salmonids : hepatic glucokinase is expressed in wild fish in Kerguelen Islands
- Author
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Lucie Marandel, Stéphane Glise, Vincent Veron, Alexandre Herman, Stéphane Panserat, Jacques Labonne, Elisabeth Plagnes-Juan, Francois Gueraud, Philippe Gaudin, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Ecologie Comportementale et Biologie des Populations de Poissons (ECOBIOP), and This work benefited from the financial support of the Fédération de recherche MIRA (Milieu et ressources aquatiques) on behalf of University of Pau and Pays de l'Adour. This study is part of SALMEVOL 1041 program, funded by the French Polar Institute Paul Emile Victor (IPEV).
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0106 biological sciences ,0301 basic medicine ,Environmental Engineering ,glucose metabolism ,Antarctic Regions ,Carbohydrate metabolism ,010603 evolutionary biology ,01 natural sciences ,poisson sauvage ,03 medical and health sciences ,Brown trout ,Nutrient ,salmonids ,Dietary Carbohydrates ,Animals ,Environmental Chemistry ,polar ,14. Life underwater ,métabolisme du glucose ,Waste Management and Disposal ,Salmonidae ,ComputingMilieux_MISCELLANEOUS ,Trophic level ,gluconeogénèse ,Islands ,salmonidae ,glucokinase ,biology ,Ecology ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,biology.organism_classification ,Pollution ,environnement ,Diet ,Trout ,030104 developmental biology ,gluconeogenesis ,Liver ,carbohydrate ,Predatory Behavior ,Freshwater fish ,kerguelen islands ,glyconeogenesis ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,iles kerguelen ,environment - Abstract
International audience; Salmonids belong to a high trophic level and are thus considered as strictly carnivorous species, metabolically adapted for high catabolism of proteins and low utilisation of dietary carbohydrates. However they conserved a “mammalian-type” nutritional regulation of glucokinase encoding gene and its enzymatic activity by dietary carbohydrates which remains puzzling regarding their dietary regime. The present study investigates the hypothesis that this conservation could be linked to a real consumption by trout of this nutrient in their natural habitat. To do so, brown trout were sampled in the sub-Antarctic Kerguelen Islands, a site presenting oligotrophic hydrosystems and no local freshwater fish fauna prior the introduction of salmonids fifty years ago. Qualitative and quantitative analysis of carbohydrate content within Kerguelen trout stomachs demonstrate that these animals are fed on food resources containing digestible carbohydrates. Additionally, glycaemia and more particularly gck mRNA level and gck enzymatic activity prove that Kerguelen trout digest and metabolise dietary carbohydrates. Physiological and molecular analyses performed in the present study thus strongly evidence for consumption of dietary carbohydrates by wild trout in natural environments. Investigating differences between Kerguelen individuals, we found that smaller individuals presented higher glycaemia, as well as higher carbohydrates contents in stomach. However no relationship between scaled mass index and any physiological indicator was found. Thus it appears that Kerguelen trout do not turn to carbohydrate diet because of a different condition index, or that the consumption of carbohydrates does not lead to a generally degraded physiological status. As a conclusion, our findings may explain the evolutionary conservation of a “mammalian-type” nutritional regulation of gck by dietary carbohydrates in these carnivorous fish
- Published
- 2018
34. Amino Acids Attenuate Insulin Action on Gluconeogenesis and Promote Fatty Acid Biosynthesis via mTORC1 Signaling Pathway in trout Hepatocytes
- Author
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Sandrine Skiba-Cassy, Weiwei Dai, Elisabeth Plagnes-Juan, Iban Seiliez, Stéphane Panserat, Nutrition, Aquaculture et Génomique (NUAGE), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA), W. Dai gratefully acknowledges the financial assistance provided by the China Scholarship Council (CSC, File No. 2011633111) for his doctoral fellowship, and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)
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Trout ,Physiology ,mTORC1 ,biosynthèse ,p38 Mitogen-Activated Protein Kinases ,lcsh:Physiology ,0302 clinical medicine ,lcsh:QD415-436 ,Phosphorylation ,Animal biology ,chemistry.chemical_classification ,0303 health sciences ,oncorhynchus mykiss ,acide gras ,lcsh:QP1-981 ,TOR Serine-Threonine Kinases ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,Fatty Acids ,Ribosomal Protein S6 Kinases, 70-kDa ,Fatty acid biosynthesis ,amino acid ,mtorc1 ,insulin action ,fatty acid biosynthesis ,gluconeogenesis ,rainbow trout ,acide aminé ,Rainbow trout ,Biochemistry ,Amino acids ,animal feeding ,Signal Transduction ,expression des gènes ,insulin ,alimentation animale ,Primary Cell Culture ,P70-S6 Kinase 1 ,Mechanistic Target of Rapamycin Complex 1 ,Biology ,lcsh:Biochemistry ,03 medical and health sciences ,Biologie animale ,Animals ,Protein kinase B ,insuline ,030304 developmental biology ,gluconeogénèse ,Sirolimus ,Insulin action ,Lipogenesis ,Ribosomal Protein S6 Kinases ,Gluconeogenesis ,Fatty acid ,IRS1 ,Insulin receptor ,Gene Expression Regulation ,chemistry ,Multiprotein Complexes ,Hepatocytes ,Insulin Receptor Substrate Proteins ,biology.protein ,fatty acid ,glyconeogenesis ,biosynthesis ,Proto-Oncogene Proteins c-akt ,030217 neurology & neurosurgery - Abstract
Background/Aims: Carnivores exhibit poor utilization of dietary carbohydrates and glucose intolerant phenotypes, yet it remains unclear what are the causal factors and underlying mechanisms. We aimed to evaluate excessive amino acids (AAs)-induced effects on insulin signaling, fatty acid biosynthesis and glucose metabolism in rainbow trout and determine the potential involvement of mTORC1 and p38 MAPK pathway. Methods: We stimulated trout primary hepatocytes with different AA levels and employed acute administration of rapamycin to inhibit mTORC1 activation. Results: Increased AA levels enhanced the phosphorylation of ribosomal protein S6 kinase (S6K1), S6, and insulin receptor substrate 1 (IRS-1) on Ser302 but suppressed Akt and p38 phosphorylation; up-regulated the expression of genes related to gluconeogenesis and fatty acid biosynthesis. mTORC1 inhibition not only inhibited the phosphorylation of mTORC1 downstream targets, but also blunted IRS-1 Ser302 phosphorylation and restored excessive AAs-suppressed Akt phosphorylation. Rapamycin also inhibited fatty acid biosynthetic and gluconeogenic gene expression. Conclusion: High levels of AAs up-regulate hepatic fatty acid biosynthetic gene expression through an mTORC1-dependent manner, while attenuate insulin-mediated repression of gluconeogenesis through elevating IRS-1 Ser302 phosphorylation, which in turn impairs Akt activation and thereby weakening insulin action. We propose that p38 MAPK probably also involves in these AAs-induced metabolic changes.
- Published
- 2015
35. Muscle catabolic capacities and global hepatic epigenome are modified in juvenile rainbow trout fed different vitamin levels at first feeding
- Author
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Lucie Marandel, Eva Arbenoits, Ester Santigosa, Karine Dias, Elisabeth Plagnes-Juan, Stéphane Panserat, Vincent Veron, Gaia Pegourié, Viviane Verlhac Trichet, Inge Geurden, Gilbert Weber, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Research Center for Animal Nutrition and Health, DSM Nutritional Products France, DSM Nutritional Products Ltd, Research Centre for Animal Nutrition and Health, and This work was fully supported by 2 research projects INRA - DSM (2013–2015) entitled 'Nutritional programming in fish: effects of early intake of vitamins on growth and metabolism in rainbow trout juveniles'
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0301 basic medicine ,Vitamin ,medicine.medical_specialty ,030209 endocrinology & metabolism ,energy and lipid catabolism ,Aquatic Science ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,épigénétique ,catabolisme ,rainbow ,lipid ,Internal medicine ,medicine ,Citrate synthase ,14. Life underwater ,vitamine ,lipide ,énergie ,2. Zero hunger ,trout ,biology ,epigenetics ,Catabolism ,Glutamate dehydrogenase ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,vitamin ,Lipid metabolism ,Metabolism ,programmation nutritionnelle ,biology.organism_classification ,rainbow trout ,Trout ,030104 developmental biology ,Endocrinology ,chemistry ,biology.protein ,nutritional programming ,metabolism ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,Pyruvate kinase ,energy ,truite arc en ciel - Abstract
Based on the concept of nutritional programming in mammals, we tested whether a short term hyper or hypo vitamin stimulus during first-feeding could induce long-lasting changes in nutrient metabolism in rainbow trout. Trout alevins received during the 4 first weeks of exogenous feeding a diet either without supplemental vitamins (NOSUP), a diet supplemented with a vitamin premix to satisfy the minimal requirement in all the vitamins (NRC) or a diet with a vitamin premix corresponding to an optimal vitamin nutrition (OVN). Following a common rearing period on the control diet, all three groups were then evaluated in terms of metabolic marker gene expressions at the end of the feeding period (day 119). Whereas no gene modifications for proteins involved in energy and lipid metabolism were observed in whole alevins (short-term effect), some of these genes showed a long-term molecular adaptation in the muscle of juveniles (long-term effect). Indeed, muscle of juveniles subjected at an early feeding of the OVN diet displayed up-regulated expression of markers of lipid catabolism (3-hydroxyacyl-CoA dehydrogenase – HOAD - enzyme) and mitochondrial energy metabolism (Citrate synthase - cs, Ubiquitinol cytochrome c reductase core protein 2 - QCR2, cytochrome oxidase 4 - COX4, ATP synthase form 5 - ATP5A) compared to fish fed the NOSUP diet. Moreover, some key enzymes involved in glucose catabolism (Muscle Pyruvate kinase - PKM) and amino acid catabolism (Glutamate dehydrogenase - GDH3) were also up regulated in muscle of juvenile fish fed with the OVN diet at first-feeding compared to fish fed the NOSUP diet. We researched if these permanently modified gene expressions could be related to global modifications of epigenetic marks (global DNA methylation and global histone acetylation and methylation). There was no variation of the epigenetic marks in muscle. However, we found changes in hepatic DNA methylation, global H3 acetylation and H3K4 methylation, dependent on the vitamin intake at early life. In summary, our data show, for the first time in fish, that a short-term vitamin-stimulus during early life may durably influence muscle energy and lipid metabolism as well as some hepatic epigenetic marks in rainbow trout. Statement of relevance Programming at first feeding with different levels of vitamins was successfully tested in fish for the first time. These data proove one more time the importance of the early life on the fish metabolism in adults.
- Published
- 2017
36. Long-term programming effect of early hypoxia and high carbohydrate diet at first-feeding on glucose metabolism in rainbow trout juveniles
- Author
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Stéphane Panserat, Karine Dias, Jingwei Liu, Lucie Marandel, Vincent Veron, and Elisabeth Plagnes-Juan
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0301 basic medicine ,medicine.medical_specialty ,Physiology ,030209 endocrinology & metabolism ,Aquatic Science ,Carbohydrate metabolism ,Feed conversion ratio ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Juvenile ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics ,biology ,Glucose transporter ,Juvenile fish ,Carbohydrate ,biology.organism_classification ,Trout ,030104 developmental biology ,Endocrinology ,13. Climate action ,Insect Science ,Animal Science and Zoology ,Rainbow trout - Abstract
Environmental conditions experienced during early life play an important role in the long-term metabolic status of individuals. The present study investigated whether early hypoxia exposure (24 h, 2.5 mg·l−1, 20% dissolved O2) during embryo stage alone (hypoxic history) or combined with a 5-day high carbohydrate diet (60%) stimulus at first-feeding (high carbohydrate diet history) can affect the glucose metabolism later in life, i.e. in fish juveniles. After 19 weeks of growth, we observed a decrease in final body weight in fish with a high carbohydrate diet history. Feed efficiency was significantly affected by both hypoxic and dietary histories. After a short challenge test (5 days) performed with a 30% carbohydrate diet in trout juveniles, our results also showed that in trout which experienced hypoxic history, mRNA levels of gluconeogenic genes in liver and glucose transport genes in both liver and muscle of fish juveniles were significantly increased. Besides, mRNA levels of glycolytic genes were decreased in fish with a high carbohydrate dietary history. Both hypoxic and dietary histories barely affected plasma metabolites and global epigenetic modifications in fish juveniles after the challenge test. In conclusion, our results demonstrated that an acute hypoxic stimulus during early development alone or combined with a hyperglucidic stimulus at first feeding can modify growth performance and glucose metabolism at the molecular level in trout juveniles.
- Published
- 2017
37. Exposure to an acute hypoxic stimulus during early life affects the expression of glucose metabolism-related genes at first-feeding in trout
- Author
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Elisabeth Plagnes-Juan, Stéphane Panserat, Lucie Marandel, Jingwei Liu, Inge Geurden, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), J.L. received a doctoral fellowship from the China Scholarship Council (File No. 201506330063). This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 652831 (AQUAEXCEL2020, Aquaculture infrastructures for excellence in European fish research towards 2020, 'WP6 Early life history impact on later experimental fish performance')., and European Project: 652831
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0301 basic medicine ,Male ,Embryo, Nonmammalian ,Glycolysis ,Hypoxia ,ComputingMilieux_MISCELLANEOUS ,Animal biology ,oncorhynchus mykiss ,Multidisciplinary ,biology ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,programmation nutritionnelle ,rainbow trout ,Trout ,Alimentation et Nutrition ,Medicine ,Female ,animal feeding ,medicine.symptom ,expression des gènes ,Fish Proteins ,medicine.medical_specialty ,endocrine system ,animal structures ,alimentation animale ,Science ,Carbohydrate metabolism ,Article ,03 medical and health sciences ,Internal medicine ,Biologie animale ,medicine ,Food and Nutrition ,Animals ,RNA, Messenger ,nutrition animale ,métabolisme du glucose ,urogenital system ,Glucose transporter ,hypoxie ,Metabolism ,Hypoxia (medical) ,Carbohydrate ,biology.organism_classification ,030104 developmental biology ,Endocrinology ,Glucose ,animal nutrition ,Rainbow trout ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition - Abstract
Rainbow trout (Oncorhynchus mykiss) is considered a “glucose-intolerant” species. With the aim of programming trout to improve their metabolic use of dietary carbohydrates, we hypothesised that a hypoxic stimulus applied during embryogenesis could later affect glucose metabolism at the first-feeding stage. An acute hypoxic stimulus (2.5 or 5.0 mg·L−1 O2) was applied for 24 h to non-hatched embryos or early hatched alevins followed by a challenge test with a high carbohydrate diet at first-feeding. The effectiveness of the early hypoxic stimulus was confirmed by the induction of oxygen-sensitive markers such as egln3. At first-feeding, trout previously subjected to the 2.5 mg·L−1 O2 hypoxia displayed a strong induction of glycolytic and glucose transport genes, whereas these glucose metabolism-related genes were affected much less in trout subjected to the less severe (5.0 mg·L−1 O2) hypoxia. Our results demonstrate that an acute hypoxic stimulus during early development can affect glucose metabolism in trout at first-feeding.
- Published
- 2017
38. Long-Term Programming Effect of Embryonic Hypoxia Exposure and High-Carbohydrate Diet at First Feeding on Glucose Metabolism in Juvenile Rainbow Trout
- Author
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Jingwei Liu, Karine Dias, Elisabeth Plagnes-Juan, Vincent Veron, Stéphane Panserat, Lucie Marandel, Nutrition, Métabolisme, Aquaculture (NuMéA), and Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA)
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No keyword ,Embryo, Nonmammalian ,Glucose ,Oncorhynchus mykiss ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,Dietary Carbohydrates ,Animals ,Anaerobiosis ,ComputingMilieux_MISCELLANEOUS - Abstract
Environmental conditions experienced during early life play an important role in the long-term metabolic status of individuals. The present study investigated whether hypoxia exposure [for 24 h: 2.5 mg O
- Published
- 2017
39. Postprandial kinetics of gene expression of proteins involved in the digestive process in rainbow trout (O. mykiss) and impact of diet composition
- Author
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Françoise Médale, Christine Burel, Geneviève Corraze, Alexandre Herman, Marianne Cluzeaud, Béatrice Lauga, Elisabeth Plagnes-Juan, Marion Borey, Anne Surget, Stéphane Panserat, Viviana Lazzarotto, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM), Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), This research was supported by the emergence programme of INRA (2013) and by the ANR Fish Physiol Biochem 123 Author's personal copy AGREENFISH Project (ANR-GUI-AAP-06). In addition, the fish used in this experiment came from a long-term study including the European ARRAINA project (Advanced Research Initiatives for Nutrition and Aquaculture) (No. 288925) of the FP7 programme and by the FUI project VegeAqua, ANR-13-ADAP-0001,AGREENFISH,Adaptations aux transitions alimentaires en aquaculture: les caractériser et les favoriser(2013), European Project: 288925,EC:FP7:KBBE,FP7-KBBE-2011-5,ARRAINA(2012), Université de Pau et des Pays de l'Adour (UPPA)-Centre National de la Recherche Scientifique (CNRS), and ANR: AGREENFISH ,ANR-GUI-AAP-06
- Subjects
Blood Glucose ,0301 basic medicine ,Hydrolases ,Physiology ,Gene Expression ,Biochemistry ,Pepsin ,Food science ,Amino Acids ,Intestinal Mucosa ,Plant Proteins ,Glucose Transporter Type 2 ,2. Zero hunger ,Symporters ,biology ,04 agricultural and veterinary sciences ,General Medicine ,Postprandial Period ,Fish oil ,Trout ,Nutrient transporters ,Postprandial ,Rainbow trout ,Oncorhynchus mykiss ,Digestion ,Fish Proteins ,medicine.medical_specialty ,Aquatic Science ,Peptide Transporter 1 ,03 medical and health sciences ,Fish Oils ,Sodium-Glucose Transporter 1 ,Fish meal ,Digestive enzymes ,Internal medicine ,Fish Products ,medicine ,Animals ,Plant Oils ,[CHIM]Chemical Sciences ,Gastric lipase ,14. Life underwater ,Triglycerides ,biology.organism_classification ,Diet ,Plant-based diet ,030104 developmental biology ,Endocrinology ,Gastric Mucosa ,040102 fisheries ,biology.protein ,0401 agriculture, forestry, and fisheries - Abstract
International audience; The impact of increased incorporation of plant ingredients on diets for rainbow trout was evaluated in terms of gene expression of gastric (gastric lipase, pepsinogen) and intestinal (prolidase, maltase, phospholipase A2) digestive enzymes and nutrient transporters (peptide and glucose transporters), as well as of postprandial levels of plasma glucose, triglycerides and total free amino acids. For that purpose, trout alevins were fed from the start of exogenous feeding one of three different experimental diets: a diet rich in fish meal and fish oil (FM–FO), a plant-based diet (noFM–noFO) totally free from fish meal and fish oil, but containing plant ingredients and a Mixed diet (Mixed) intermediate between the FM–FO and noFM–noFO diets. After 16 months of rearing, all fish were left unfed for 72 h and then given a single meal to satiation. Blood, stomach and anterior intestine were sampled before the meal and at 2, 6 and 12 h after this meal. The postprandial kinetics of gene expression of gastric and intestinal digestive enzymes and nutrient transporters were then followed in trout fed the FM–FO diet. The postprandial profiles showed that the expression of almost all genes studied was stimulated by the presence of nutrients in the digestive tract of trout, but the timing (appearance of peaks) varied between genes. Based on these data, we have focused on the molecular response to dietary factors in the stomach and the intestine at 6 and 12 h after feeding, respectively. The reduction in FM and FO levels of dietary incorporation induced a significant decrease in the gene expression of gastric lipase, GLUT2 and PEPT1. The plasma glucose and triglycerides levels were also reduced in trout fed the noFM–noFO diet. Consequently, the present study suggests a decrease in digestive capacities in trout fed a diet rich in plant ingredients.
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- 2016
40. Hepatic fatty acid biosynthesis is more responsive to protein than carbohydrate in rainbow trout during acute stimulations
- Author
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Weiwei Dai, Elisabeth Plagnes-Juan, Iban Seiliez, Sadasivam Kaushik, Frédéric Terrier, Stéphane Panserat, Sandrine Skiba-Cassy, Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), and W. Dai gratefully acknowledges the financial assistance provided by the China Scholarship Council (CSC, file no. 2011633111) for his doctoral fellowship
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0301 basic medicine ,target of rapamycin ,Time Factors ,food intake ,ATP citrate lyase ,Physiology ,fatty acid biosynthesis ,rapamycine ,biosynthèse ,poisson ,Insulin ,Amino Acids ,Cells, Cultured ,lipogénèse ,chemistry.chemical_classification ,trout ,oncorhynchus mykiss ,alimentation ,biology ,acide gras ,TOR Serine-Threonine Kinases ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,Fatty Acids ,rainbow trout ,Pyruvate carboxylase ,Fatty acid synthase ,sirolimus ,Biochemistry ,protéine ,Lipogenesis ,Animal Nutritional Physiological Phenomena ,Dietary Proteins ,Signal Transduction ,Fish Proteins ,medicine.medical_specialty ,Primary Cell Culture ,Nutritional Status ,Mechanistic Target of Rapamycin Complex 1 ,Gene Expression Regulation, Enzymologic ,03 medical and health sciences ,rainbow ,Physiology (medical) ,Internal medicine ,medicine ,Dietary Carbohydrates ,Animals ,nutrition animale ,Protein Kinase Inhibitors ,lipogenesis ,fish ,Fatty acid ,Carbohydrate ,Sterol regulatory element-binding protein ,glucide ,030104 developmental biology ,Endocrinology ,Enzyme ,Glucose ,chemistry ,carbohydrate ,Multiprotein Complexes ,animal nutrition ,biology.protein ,Hepatocytes ,fatty acid ,biosynthesis ,protein ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,truite arc en ciel - Abstract
The link between dietary carbohydrate/protein and de novo lipogenesis (DNL) remains debatable in carnivorous fish. We aimed to evaluate and compare the response of hepatic lipogenic gene expression to dietary carbohydrate intake/glucose and dietary protein intake/amino acids (AAs) during acute stimulations using both in vivo and in vitro approaches. For the in vivo trial, three different diets and a controlled-feeding method were employed to supply fixed amount of dietary protein or carbohydrate in a single meal; for the in vitro trial, primary hepatocytes were stimulated with a low or high level of glucose (3 mM or 20 mM) and a low or high level of AAs (one-fold or four-fold concentrated AAs). In vitro data showed that a high level of AAs upregulated the expression of enzymes involved in DNL [fatty acid synthase (FAS) and ATP citrate lyase (ACLY)], lipid bioconversion [elongation of very long chain fatty acids like-5 (Elovl5), Elovl2, Δ6 fatty acyl desaturase (D6D) and stearoyl-CoA desaturase-1 (SCD1)], NADPH production [glucose-6-phosphate dehydrogenase (G6PDH) and malic enzyme (ME)], and transcriptional factor sterol regulatory element binding protein 1-like, while a high level of glucose only elevated the expression of ME. Data in trout liver also showed that high dietary protein intake induced higher lipogenic gene expression (FAS, ACLY, and Elovl2) regardless of dietary carbohydrate intake, while high carbohydrate intake markedly suppressed the expression of acetyl-CoA carboxylase (ACC) and Elovl5. Overall, we conclude that, unlike rodents or humans, hepatic fatty acid biosynthetic gene expression in rainbow trout is more responsive to dietary protein intake/AAs than dietary carbohydrate intake/glucose during acute stimulations. This discrepancy probably represents one important physiological and metabolic difference between carnivores and omnivores.
- Published
- 2016
41. Apparent low ability of liver and muscle to adapt to variation of dietary carbohydrate:protein ratio in rainbow trout (Oncorhynchus mykiss)
- Author
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Mélanie Larquier, Sadasivam Kaushik, Elisabeth Plagnes-Juan, Karine Dias, Stéphane Panserat, Anne Surget, Sandrine Skiba-Cassy, Iban Seiliez, Nutrition, Métabolisme, Aquaculture (NUMEA), Institut National de la Recherche Agronomique (INRA), and ANR-08-JCJC-0025
- Subjects
medicine.medical_specialty ,Low protein ,[SDV]Life Sciences [q-bio] ,Gene Expression ,Medicine (miscellaneous) ,030209 endocrinology & metabolism ,03 medical and health sciences ,0302 clinical medicine ,Carnitine palmitoyltransferase 1 ,Internal medicine ,Glucokinase ,Diet, Protein-Restricted ,Dietary Carbohydrates ,medicine ,Animals ,[INFO]Computer Science [cs] ,signalling ,Phosphorylation ,030304 developmental biology ,Analysis of Variance ,0303 health sciences ,Nutrition and Dietetics ,biology ,Chemistry ,Futile cycle ,Muscles ,Metabolism ,biology.organism_classification ,rainbow trout ,enzyme activity ,Fishery ,Trout ,Endocrinology ,Liver ,Hyperglycemia ,Oncorhynchus mykiss ,Rainbow trout ,Dietary Proteins ,metabolism ,Glycolysis ,Metabolic Networks and Pathways ,Pyruvate kinase - Abstract
The rainbow trout (Oncorhynchus mykiss) exhibits high dietary amino acid requirements and an apparent inefficiency to use dietary carbohydrates. Using this species, we investigated the metabolic consequences of long-term high carbohydrates/low protein feeding. Fish were fed two experimental diets containing either 20 % carbohydrates/50 % proteins (C20P50), or high levels of carbohydrates at the expense of proteins (35 % carbohydrates/35 % proteins – C35P35). The expression of genes related to hepatic and muscle glycolysis (glucokinase (GK), pyruvate kinase and hexokinase) illustrates the poor utilisation of carbohydrates irrespective of their dietary levels. The increased postprandial GK activity and the absence of inhibition of the gluconeogenic enzyme glucose-6-phosphatase activity support the hypothesis of the existence of a futile cycle around glucose phosphorylation extending postprandial hyperglycaemia. After 9 weeks of feeding, the C35P35-fed trout displayed lower body weight and feed efficiency and reduced protein and fat gains than those fed C20P50. The reduced activation of eukaryotic translation initiation factor 4-E binding protein 1 (4E-BP1) in the muscle in this C35P35 group suggests a reduction in protein synthesis, possibly contributing to the reduction in N gain. An increase in the dietary carbohydrate:protein ratio decreased the expression of genes involved in amino acid catabolism (serine dehydratase and branched-chain α-keto acid dehydrogenase E1α and E1β), and increased that of carnitine palmitoyltransferase 1, suggesting a higher reliance on lipids as energy source in fish fed high-carbohydrate and low-protein diets. This probably also contributes to the lower fat gain. Together, these results show that different metabolic pathways are affected by a high-carbohydrate/low-protein diet in rainbow trout.
- Published
- 2012
42. Dietary fat level modifies the expression of hepatic genes in juvenile rainbow trout (Oncorhynchus mykiss) as revealed by microarray analysis
- Author
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François Piumi, Catherine-Ines Kolditz, Prem Prakash Srivastava, Sadasivam Kaushik, Diane Esquerre, Elisabeth Plagnes-Juan, Stéphane Panserat, S. Ducasse-Cabanot, Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Central Institute of Fisheries Education, Laboratoire de radiobiologie et d'étude du génome (LREG), Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)
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medicine.medical_specialty ,LIVER ,030309 nutrition & dietetics ,Dietary lipid ,Aquatic Science ,Transcriptome ,03 medical and health sciences ,Aquaculture ,RAINBOW TROUT ,Lipid biosynthesis ,Internal medicine ,FISH NUTRITION ,medicine ,14. Life underwater ,030304 developmental biology ,0303 health sciences ,biology ,business.industry ,Microarray analysis techniques ,Fish oil ,biology.organism_classification ,GENOMIQUE ,Trout ,Endocrinology ,Biochemistry ,INTERMEDIARY METABOLISM ,[SDV.SA.STP]Life Sciences [q-bio]/Agricultural sciences/Sciences and technics of fishery ,Rainbow trout ,business ,GENOMICS ,SALMONIDE - Abstract
International audience; The objective of the present study was to identify postprandial molecular events associated with dietary fish oil level in rainbow trout using trout cDNA microarray tools (9 K). Our genomics data showed that relatively few hepatic genes (n=41) are differentially expressed between fish fed a high fat diet compared to those fed a diet without added fish oil. We then focus our analysis of specific events involved in intermediary metabolism by analysing them using qRT-PCR. Removal of fish oil was associated with higher lipid biosynthesis (reflected by the fatty acid synthase gene) and lower lipid catabolism (reflected by the acyl-CoA oxidase gene). More interestingly, some genes involved in proteasomal-dependant proteolysis (proteasome and ubiquitin ligase genes) were down regulated in fish fed without fish oil, suggesting a relationship between dietary lipid intake and regulation of hepatic protein metabolism in rainbow trout. (c) 2008 Elsevier B.V. All rights reserved.
- Published
- 2008
43. Glucose metabolism ontogenesis in rainbow trout (Oncorhynchus mykiss) in the light of the recently sequenced genome: new tools for intermediary metabolism programming
- Author
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Vincent Veron, Elisabeth Plagnes-Juan, Lucie Marandel, Stéphane Panserat, Anne Surget, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), This research was internally funded by the European Commission (European project FP7-KBBE-2011-5, ARRAINA project no. 288925, Advanced Research Initiatives for Nutrition and Aquaculture), and European Project: 288925,EC:FP7:KBBE,FP7-KBBE-2011-5,ARRAINA(2012)
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0301 basic medicine ,Physiology ,Zoology ,Aquatic Science ,Carbohydrate metabolism ,03 medical and health sciences ,No keyword ,Dietary Carbohydrates ,Animals ,14. Life underwater ,Molecular Biology ,Gene ,Ecology, Evolution, Behavior and Systematics ,ComputingMilieux_MISCELLANEOUS ,Phylogeny ,Genome ,biology ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,Embryogenesis ,Gene Expression Regulation, Developmental ,Embryo ,biology.organism_classification ,Phenotype ,Trout ,030104 developmental biology ,Glucose ,Gluconeogenesis ,Biochemistry ,Liver ,Insect Science ,Larva ,Oncorhynchus mykiss ,Glucose-6-Phosphatase ,Animal Science and Zoology ,Rainbow trout - Abstract
The rainbow trout (Oncorhynchus mykiss), a carnivorous fish species, displays a “glucose-intolerant” phenotype when fed a high carbohydrate diet. The importance of carbohydrate metabolism during embryogenesis and the timing of establishing this latter phenotype are currently unclear. In addition, the mechanisms underlying the poor ability of carnivorous fish to use dietary carbohydrates as a major energy substrate are poorly understood. It has recently been shown in trout that duplicated genes involved in glucose metabolism may be involved in establishing the glucose-intolerant phenotype. The aim of this study was therefore to provide new understanding of glucose metabolism during ontogenesis and nutritional transition, taking into consideration the complexity of the trout genome. Trout were sampled at several stages of development from fertilization to hatching, and alevins were then fed a non-, or a high carbohydrate diet during first-feeding. mRNA levels of all glucose metabolism-related genes increased in embryos during the setting up of the primitive liver. After the first meal, genes rapidly displayed expression patterns equivalent to those observed in the livers of juveniles. g6pcb2.a (one glucose-6-phosphatase encoding gene) was up-regulated in alevins fed a high carbohydrate diet, mimicking the expression pattern of gck genes. The g6pcb2.a gene may contribute to the non-inhibition of the last step of gluconeogenesis and thus to establishing the glucose-intolerant phenotype in trout fed a high carbohydrate diet as early as first-feeding. This information is crucial for nutritional programming investigations as it suggests that first feeding would be too late to programme glucose metabolism in the long term.
- Published
- 2015
44. Acute endocrine and nutritional co-regulation of the hepatic omy-miRNA-122b and the lipogenic gene fas in rainbow trout, Oncorhynchus mykiss
- Author
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Elisabeth Plagnes-Juan, Claudia A. Figueredo-Silva, Sandrine Skiba-Cassy, Jan A. Mennigen, Iban Seiliez, Stéphane Panserat, Nutrition, Métabolisme, Aquaculture (NUMEA), Institut National de la Recherche Agronomique (INRA), Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), and The authors would like to acknowledge funding through a Marie-Curie Post-doctoral Fellowship to JAM (project reference 273840, and the Seventh Framework Programme of the European Commission, http://cordis.europa.eu)
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medicine.medical_specialty ,insulin ,Physiology ,medicine.medical_treatment ,liver ,Biochemistry ,In vivo ,Internal medicine ,microRNA ,Dietary Carbohydrates ,medicine ,Animals ,Endocrine system ,Molecular Biology ,lipogenesis ,biology ,Insulin ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,intermediary metabolism ,biology.organism_classification ,Dietary Fats ,MicroRNAs ,Trout ,Endocrinology ,Postprandial ,Oncorhynchus mykiss ,Lipogenesis ,Animal Nutritional Physiological Phenomena ,Rainbow trout ,Dietary Proteins ,Fatty Acid Synthases - Abstract
International audience; Hepatic lipogenesis represents a crucial part of intermediary metabolism and is acutely regulated by endocrine factors and nutrients. The liver-specific and highly abundant microRNA-122 has emerged as a powerful regulator of lipogenesis in higher vertebrates, but little is known about its endocrine and nutritional regulation. In this study, we investigated the hypothesis that insulin regulates hepatic expression of omy-miRNA-122 isomiRNAs (omy-miRNA-122a and omy-miRNA-122b) by using in vivo and in vitro approaches. Since the hepatic insulin pathway and lipogenesis are acutely regulated by dietary macronutrient ratios in rainbow trout, we further investigated the effect of single meals with altered carbohydrate/protein ratio and lipid/protein ratio on the postprandial expression of omy-miRNA-122 isomiRNAs. Insulin acutely induced omy-miRNA-122b expression in vivo and in vitro. Conversely, a single meal with increased lipid to protein ratio acutely decreased expression of both omy-miRNA-122 isomiRNAs. As a direct proof of lipogenic effects of miRNA-122 is currently still lacking in fish, we investigated the correlated expression between omy-miRNA-122 isomiRNAs and the rate-limiting lipogenic gene fas, an indirect target gene of miRNA-122 in mammals. Our results show a significant positive correlation of omy-miRNA-122b and fas, consistent with a potential evolutionary conserved role for miRNA-122 in the regulation of postprandial lipogenesis in trout
- Published
- 2014
45. Glucose metabolic gene expression in growth hormone transgenic coho salmon
- Author
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Jeanne Fournier, Biju Sam Kamalam, Stéphane Panserat, Elisabeth Plagnes-Juan, Krista Woodward, Robert H. Devlin, Nutrition, Métabolisme, Aquaculture (NUMEA), Institut National de la Recherche Agronomique (INRA), Fisheries and Oceans Canada (DFO), and This study has been supported by funds from INRA (National insti- tute of Agronomic Research, France) and from the Canadian Regulatory System for Biotechnology. The second author gratefully acknowledges the fi nancial assistance provided by the Indian Council of Agricultural Research (ICAR) through the ICAR International Fellowship for his doctoral programme
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medicine.medical_specialty ,Physiology ,Transgene ,glucose metabolism ,Carbohydrate metabolism ,Glucosephosphate Dehydrogenase ,Biochemistry ,Animals, Genetically Modified ,Internal medicine ,Glucokinase ,medicine ,Glucose homeostasis ,Animals ,RNA, Messenger ,Molecular Biology ,Glucose tolerance test ,Glucose Transporter Type 4 ,biology ,medicine.diagnostic_test ,Muscles ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,Glucose transporter ,salmon ,Oncorhynchus kisutch ,Endocrinology ,Glucose ,Gene Expression Regulation ,Liver ,Lipogenesis ,growth hormone ,biology.protein ,transgenic fish ,GLUT4 - Abstract
International audience; Salmonids are generally known to be glucose intolerant.However,previous studies have shown that growth hor- mone (GH) transgenic coho salmon display modified nutritional regulation of glycolysis and lipogenesis com- pared to non-transgenic fish, suggesting the potential for better use of glucose in GH transgenic fish. To examine this in detail, GH transgenic and non-transgenic coho salmon were subjected to glucose tolerance test and subsequent metabolic assessments. After intra-peritoneal injection of 250 mg/kg glucose, we analysed post-injectionkineticsofglycaemiaandexpressionofseveralkeytargetgeneshighlyinvolvedinglucosehomeo- stasis in muscle and liver tissues. Our data show no significant differences in plasma glucose levels during peak hyperglycaemia (3–6 h after injection), demonstrating a similar glucose tolerance between transgenic and non transgenic. However, and unrelated to the hyperglycaemic episode, GH transgenic fish return to a slightly lower basal glycaemia values 24 h after injection. Correspondingly, GH transgenic fish exhibited higher mRNA levels of glucokinase (GK) and glucose-6-phosphate dehydrogenase (G6PDH) in liver, and glucose transporter (GLUT4) in muscle. These data suggest that these metabolic actors may be involved in different glucose use in GHtransgenic fish,which would be expected to influence the glucose challenge response.Overall,our data dem- onstrate that GH transgenic coho salmon may be a pertinent animal model for further study of glucose metabo- lism in carnivorous fish
- Published
- 2014
46. The metabolic consequences of hepatic AMP-kinase phosphorylation in rainbow trout
- Author
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Elisabeth Plagnes-Juan, Stéphane Panserat, David J. Martyres, Thomas W. Moon, Sergio Polakof, Sharareh Savari, Stéphane Aris-Brosou, Paul M. Craig, Nutrition, Métabolisme, Aquaculture (NUMEA), Institut National de la Recherche Agronomique (INRA), Laboratorio de Fisioloxia Animal, Universidade de Vigo, and University of Ottawa [Ottawa]
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AMPK ,Anatomy and Physiology ,Animal Evolution ,Gene Expression ,lcsh:Medicine ,Aquaculture ,Biochemistry ,0302 clinical medicine ,poisson ,Integrative Physiology ,Homeostasis ,Threonine ,lcsh:Science ,ComputingMilieux_MISCELLANEOUS ,salmonidae ,0303 health sciences ,oncorhynchus mykiss ,Multidisciplinary ,Kinase ,phosphorylation ,Agriculture ,foie ,Lipids ,Enzymes ,Fatty acid synthase ,Liver ,Phosphorylation ,Carbohydrate Metabolism ,Metabolic Pathways ,Research Article ,Cell Physiology ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,Biology ,Bioenergetics ,03 medical and health sciences ,RAINBOW TROUT ,Chemical Biology ,Genetics ,Animals ,Protein kinase A ,métabolisme ,AMP KINASE ,LIVER ,SALMONIDE ,030304 developmental biology ,Evolutionary Biology ,Glucokinase ,Evolutionary Developmental Biology ,Adenylate Kinase ,lcsh:R ,Lipid metabolism ,Genomic Evolution ,Lipid Metabolism ,Organismal Evolution ,Metabolism ,biology.protein ,lcsh:Q ,Physiological Processes ,Energy Metabolism ,030217 neurology & neurosurgery ,truite arc en ciel - Abstract
AMP-activated protein kinase (AMPK), a phylogenetically conserved serine/threonine protein kinase, is proposed to function as a “fuel gauge” to monitor cellular energy status in response to nutritional environmental variations. However, in fish, few studies have addressed the metabolic consequences related to the activation of this kinase. This study demonstrates that the rainbow trout (Oncorhynchus mykiss) possesses paralogs of the three known AMPK subunits that co-diversified, that the AMPK protein is present in the liver and in isolated hepatocytes, and it does change in response to physiological (fasting-re-feeding cycle) and pharmacological (AICAR and metformin administration and incubations) manipulations. Moreover, the phosphorylation of AMPK results in the phosphorylation of acetyl-CoA carboxylase, a main downstream target of AMPK in mammals. Other findings include changes in hepatic glycogen levels and several molecular actors involved in hepatic glucose and lipid metabolism, including mRNA transcript levels for glucokinase, glucose-6-phosphatase and fatty acid synthase both in vivo and in vitro. The fact that most results presented in this study are consistent with the recognized role of AMPK as a master regulator of energy homeostasis in living organisms supports the idea that these functions are conserved in this piscine model.
- Published
- 2011
47. L-Leucine, L-Methionine, and L-Lysine Are Involved in the Regulation of Intermediary Metabolism-Related Gene Expression in Rainbow Trout Hepatocytes
- Author
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Iban Seiliez, Marine Lansard, Elisabeth Plagnes-Juan, Sandrine Skiba-Cassy, Stéphane Panserat, Karine Dias, Nutrition, Métabolisme, Aquaculture (NUMEA), Institut National de la Recherche Agronomique (INRA), European Union [016249-2], French National Research Agency [ANR-08-JCJC-0025], Aquitaine Region [CCRRDT-20051303004AB], and AQUAMAX European project
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Male ,LIVER ,[SDV]Life Sciences [q-bio] ,INSULIN-RESPONSE ,Medicine (miscellaneous) ,CHAIN AMINO-ACIDS ,chemistry.chemical_compound ,Serine dehydratase ,Methionine ,Glucokinase ,Phosphorylation ,Cells, Cultured ,chemistry.chemical_classification ,Regulation of gene expression ,0303 health sciences ,Nutrition and Dietetics ,MOLECULAR-MECHANISMS ,TOR Serine-Threonine Kinases ,MTOR ,04 agricultural and veterinary sciences ,TOR ,Amino acid ,Biochemistry ,Oncorhynchus mykiss ,Glucose-6-Phosphatase ,Phosphoenolpyruvate Carboxykinase (GTP) ,Leucine ,SIGNALING PATHWAYS ,03 medical and health sciences ,Animals ,[INFO]Computer Science [cs] ,RNA, Messenger ,030304 developmental biology ,Lysine ,Ribosomal Protein S6 Kinases ,Metabolism ,PROTEIN-KINASE ,ONCORHYNCHUS-MYKISS ,chemistry ,Gluconeogenesis ,Gene Expression Regulation ,040102 fisheries ,Hepatocytes ,0401 agriculture, forestry, and fisheries ,PANCREATIC BETA-CELLS ,Amino Acids, Essential ,Proto-Oncogene Proteins c-akt - Abstract
International audience; Using rainbow trout hepatocytes stimulated with L-leucine, L-methionine, or L-lysine in the presence or absence of bovine insulin, we investigated the ability of these amino acids to mimic the effects of a pool of amino acids on protein kinase B (Akt)/target of rapamycin (TOR) signaling pathways and expression of 6 genes known to be subjected to insulin and/or amino acid regulation [glucose-6-phosphatase (G6Pase), phosphoenolpyruvate carboxykinase (PEPCK), glucokinase (GK), pyruvate kinase (PK), fatty acid synthase (FAS), and serine dehydratase (SDH)]. Emphasis was placed on leucine, known to be a signaling molecule in mammals, and methionine and lysine that are essential amino acids limiting in plant-based diets for fish. In the presence of insulin, leucine (but not methionine or lysine) phosphorylated Akt and ribosomal protein 56 as previously observed with a pool of amino acids, suggesting that leucine might participate in the activation of the TOR pathway by amino acids in f sh, as in mammals. G6Pase, PEPCK, GK, and SDH gene expression were higher in leucine-treated cells compared with control cells. Leucine combined with insulin reduced G6Pase gene expression by 90% and increased FAS gene expression > 4-fold compared with the control treatment. Methionine weakly decreased G6Pase, GK, and SDH gene expression and lysine weakly but significantly decreased the mRNA level of PEPCK. Thus, leucine regulated gluconeogenesis and lipogenesis, but not glycolysis, in the same way as a pool of amino acids. Methionine appeared to be involved in the regulation of specific genes, whereas lysine only had limited effects. These findings are particularly relevant regarding the involvement of amino acids in the regulation of metabolism-related gene expression. J. Nutr. 141: 75-80, 2011.
- Published
- 2011
48. Dietary carbohydrate-to-protein ratio affects TOR signaling and metabolism-related gene expression in the liver and muscle of rainbow trout after a single meal
- Author
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Sergio Polakof, Iban Seiliez, Marine Lansard, Anne Surget, Elisabeth Plagnes-Juan, Mélanie Larquier, Stéphane Panserat, Karine Dias, Sandrine Skiba-Cassy, Sadasivam Kaushik, Nutrition, Métabolisme, Aquaculture (NUMEA), Institut National de la Recherche Agronomique (INRA), Laboratorio de Fisioloxia Animal, and Universidade de Vigo
- Subjects
Blood Glucose ,Fish Proteins ,medicine.medical_specialty ,Time Factors ,Physiology ,INSULINE ,Gene Expression Regulation, Enzymologic ,03 medical and health sciences ,Carnitine palmitoyltransferase 1 ,Physiology (medical) ,Internal medicine ,Dietary Carbohydrates ,medicine ,Animals ,RNA, Messenger ,Muscle, Skeletal ,030304 developmental biology ,2. Zero hunger ,Regulation of gene expression ,chemistry.chemical_classification ,0303 health sciences ,biology ,Glucokinase ,TOR Serine-Threonine Kinases ,04 agricultural and veterinary sciences ,Metabolism ,INSULIN ,TOR signaling ,Amino acid ,Fatty acid synthase ,Endocrinology ,Liver ,Biochemistry ,chemistry ,Oncorhynchus mykiss ,040102 fisheries ,biology.protein ,0401 agriculture, forestry, and fisheries ,AMINO ACID SIGNALING PATHWAY ,Dietary Proteins ,Energy Metabolism ,Proto-Oncogene Proteins c-akt ,GLUT4 ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology ,Signal Transduction ,SALMONIDE - Abstract
International audience; Most teleost fish are known to require high levels of dietary proteins. Such high-protein intake could have significant effects, particularly on insulin-regulated gene expression. We therefore analyzed the effects of an increase in the ratio of dietary carbohydrates/proteins on the refeeding activation of the Akt-target of rapamycin (TOR) signaling pathways in rainbow trout and the effects on the expression of several genes related to hepatic and muscle metabolism and known to be regulated by insulin, amino acids, and/or glucose. Fish were fed once one of three experimental diets containing high (H), medium (M), or low (L) protein (P) or carbohydrate (C) levels after 48 h of feed deprivation. Activation of the Akt/TOR signaling pathway by refeeding was severely impaired by decreasing the proteins-to-carbohydrates ratio. Similarly, postprandial regulation of several genes related to glucose (Glut4, glucose-6-phosphatase isoform 1), lipid (fatty acid synthase, ATP-citrate lyase, sterol responsive element binding protein, carnitine palmitoyltransferase 1, and 3-hydroxyacyl-CoA dehydrogenase), and amino acid metabolism (serine dehydratase and branched-chain alpha-keto acid dehydrogenase E2 subunit) only occurred when fish were fed the high-protein diet. On the other hand, diet composition had a low impact on the expression of genes related to muscle protein degradation. Interestingly, glucokinase was the only gene of those monitored whose expression was significantly upregulated by increased carbohydrate intake. In conclusion, this study demonstrated that macro-nutrient composition of the diet strongly affected the insulin/amino acids signaling pathway and expression pattern of genes related to metabolism.
- Published
- 2011
49. Integration of insulin and amino acid signals that regulate hepatic metabolism-related gene expression in rainbow trout: role of TOR
- Author
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Sandrine Skiba-Cassy, Elisabeth Plagnes-Juan, Stéphane Panserat, Marine Lansard, Iban Seiliez, Nutrition, Aquaculture et Génomique (NUAGE), and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)
- Subjects
Fish Proteins ,[SDV]Life Sciences [q-bio] ,Clinical Biochemistry ,Gene Expression ,Protein Serine-Threonine Kinases ,METABOLISM ,Biochemistry ,03 medical and health sciences ,Serine dehydratase ,AMINO ACIDS ,Animals ,Glycolysis ,[INFO]Computer Science [cs] ,Cells, Cultured ,030304 developmental biology ,HEPATIC GENE EXPRESSION ,chemistry.chemical_classification ,0303 health sciences ,biology ,Glucokinase ,TOR Serine-Threonine Kinases ,Organic Chemistry ,Intracellular Signaling Peptides and Proteins ,04 agricultural and veterinary sciences ,Metabolism ,TOR ,INSULIN ,Amino acid ,Fatty acid synthase ,Liver ,Gluconeogenesis ,chemistry ,Oncorhynchus mykiss ,Hepatocytes ,040102 fisheries ,biology.protein ,0401 agriculture, forestry, and fisheries ,Pyruvate kinase ,Signal Transduction ,SALMONIDE - Abstract
International audience; Amino acids are considered to be regulators of metabolism in several species, and increasing importance has been accorded to the role of amino acids as signalling molecules regulating protein synthesis through the activation of the TOR transduction pathway. Using rainbow trout hepatocytes, we examined the ability of amino acids to regulate hepatic metabolism-related gene expression either alone or together with insulin, and the possible involvement of TOR. We demonstrated that amino acids alone regulate expression of several genes, including glucose-6-phosphatase, phosphoenolpyruvate carboxykinase, pyruvate kinase, 6-phospho-fructo-1-kinase and serine dehydratase, through an unknown molecular pathway that is independent of TOR activation. When insulin and amino acids were added together, a different pattern of regulation was observed that depended upon activation of the TOR pathway. This pattern included a dramatic up-regulation of lipogenic (fatty acid synthase, ATP-citrate lyase and sterol responsive element binding protein 1) and glycolytic (glucokinase, 6-phospho-fructo-1-kinase and pyruvate kinase) genes in a TOR-dependent manner. Regarding gluconeogenesis genes, only glucose-6-phosphatase was inhibited in a TOR-dependent manner by combination of insulin and amino acids and not by amino acids alone. This study is the first to demonstrate an important role of amino acids in combination with insulin in the molecular regulation of hepatic metabolism.
- Published
- 2010
50. Changes in white muscle transcriptome induced by dietary energy levels in two lines of rainbow trout (Oncorhynchus mykiss) selected for muscle fat content
- Author
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Florence Lefèvre, Elisabeth Plagnes-Juan, Françoise Médale, Edwige Quillet, Catherine-Ines Kolditz, Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), and Institut National de la Recherche Agronomique (INRA)
- Subjects
CD36 Antigens ,muscle ,CD36 ,animal diseases ,Gene Expression ,Medicine (miscellaneous) ,dietary treatment ,énergie alimentaire ,Transcriptome ,poisson ,Tissue Distribution ,[SDV.BDD]Life Sciences [q-bio]/Development Biology ,salmonidae ,0303 health sciences ,oncorhynchus mykiss ,Nutrition and Dietetics ,sélection génétique ,biology ,acide gras ,Biologie du développement ,04 agricultural and veterinary sciences ,Development Biology ,Trout ,régime alimentaire ,nutrition ,Alimentation et Nutrition ,marqueur moléculaire ,Genetic Markers ,medicine.medical_specialty ,endocrine system ,DNA, Complementary ,animal structures ,VLDL receptor ,muscle lipid ,cd36 ,digestive system ,03 medical and health sciences ,fatty acid translocase/cd36 ,Transferases ,Internal medicine ,medicine ,Animals ,Food and Nutrition ,Muscle, Skeletal ,lipide ,Gene Library ,030304 developmental biology ,cDNA library ,urogenital system ,Gene Expression Profiling ,0402 animal and dairy science ,Metabolism ,Lipid Metabolism ,biology.organism_classification ,Dietary Fats ,040201 dairy & animal science ,croissance ,Diet ,genetic selection ,vldl receptor ,Endocrinology ,Receptors, LDL ,Genetic marker ,biology.protein ,RNA ,Rainbow trout ,Energy Intake ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,contrôle génétique ,truite arc en ciel - Abstract
Energy intake and genetic background are major determinants of muscle fat content in most animals, including man. We combined genetic selection and dietary energy supply to study the metabolic pathways involved in genetic and nutritional control of fat deposition in the muscle of rainbow trout (Oncorhynchus mykiss). Two experimental lines of rainbow trout, selected for lean (L) or fat (F) muscle, were fed with diets containing either 10 or 23 % lipids from the first feeding, up to 6 months. At the end of the trial, trout exhibited very different values of muscle fat content (from 4·2 to 10·1 % wet weight). Using microarrays made from a rainbow trout multi-tissue cDNA library, we analysed the molecular changes occurring in the muscle of the two lines when fed the low-energy or high-energy diet. The results from microarray analysis revealed that eleven metabolism-related genes were differentially expressed according to the diet while selection resulted in expression change for twenty-six genes. The most striking observation was the increased level of transcripts encoding the VLDL receptor and fatty acid translocase/CD36 following both the high-fat diet and upward selection for muscle fat content, suggesting that these two genes are relevant molecular markers of fat deposition in the white muscle of rainbow trout.
- Published
- 2010
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