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Your search keyword '"Elio Maximiliano Ortiz"' showing total 5 results
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5 results on '"Elio Maximiliano Ortiz"'

1. Complete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes

Author

Diego Herman Sauka, Rubén O. Zandomeni, Elio Maximiliano Ortiz, Marcelo Facundo Berretta, Ariel Fernando Amadio, Laura Emilce Navas, and Graciela Beatriz Benintende

Subjects
0301 basic medicine, Genetics, Physiology, Cell Biology, Biology, biology.organism_classification, Applied Microbiology and Biotechnology, Biochemistry, Microbiology, 03 medical and health sciences, Complete sequence, 030104 developmental biology, Plasmid, Bacillus thuringiensis, Gene, Biotechnology
Abstract

We report the complete sequence and analysis of pFR260, a novel megaplasmid of 260,595 bp from the Bacillus thuringiensis strain INTA Fr7-4 isolated in Argentina. It carries 7 insecticidal genes: 3 cry8 copies previously reported, 2 vip1, and 2 vip2. Also, it carries a gene encoding a putative atypical Cry protein. These genes are arranged in a region of approximately 105 kbp in size with characteristics of a pathogenicity island with a potential coleopteran-specific insecticide profile. DNA strand composition asymmetry, as determined by GC skew analysis, and the presence of a Rep protein involved in the initiation of replication suggest a bidirectional theta mechanism of replication. In addition, many genes involved in conjugation and a CRISPR-Cas system were detected. The pFR260 sequence was deposited in GenBank under accession number KX258624.

Published
2017

2. Draft Genome Sequence of Bacillus thuringiensis INTA Fr7-4

Author

Graciela Beatriz Benintende, Diego H. Sauka, Laura Emilce Navas, Elio Maximiliano Ortiz, Ariel Fernando Amadio, Marcelo Facundo Berretta, and Rubén O. Zandomeni

Subjects
0301 basic medicine, Genetics, Whole genome sequencing, biology, Otras Ciencias Biológicas, Strain (biology), purl.org/becyt/ford/1.2 [https], biology.organism_classification, Genome, BACILLUS THURINGIENSIS, Ciencias de la Computación, purl.org/becyt/ford/1 [https], GENOME, Ciencias Biológicas, 03 medical and health sciences, 030104 developmental biology, Ciencias de la Computación e Información, Bacillus thuringiensis, Prokaryotes, purl.org/becyt/ford/1.6 [https], Molecular Biology, Gene, CIENCIAS NATURALES Y EXACTAS
Abstract

We report here the complete annotated 6,035,547-bp draft genome sequence of Bacillus thuringiensis INTA Fr7-4. This strain contains three cry8 and two vip1 and vip2 insecticidal toxin genes. Fil: Navas, Laura Emilce. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Berretta, Marcelo Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Ortiz, Elio Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Sauka, Diego Herman. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Benintende, Graciela Beatriz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Zandomeni, Ruben O.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Amadio, Ariel Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina

Published
2017

3. Sequence and Expression of Two cry8 Genes from Bacillus thuringiensis INTA Fr7-4, a Native Strain from Argentina

Author

Graciela Beatriz Benintende, Rubén O. Zandomeni, Ariel Fernando Amadio, Melisa Paula Perez, Marcelo Facundo Berretta, Laura Emilce Navas, Elio Maximiliano Ortiz, and Diego Herman Sauka

Subjects
Cloning, Strain (chemistry), Physiology, Sequence analysis, fungi, Cell Biology, Biology, biology.organism_classification, Applied Microbiology and Biotechnology, Biochemistry, Microbiology, Molecular biology, law.invention, chemistry.chemical_compound, chemistry, law, Bacillus thuringiensis, Gene expression, Recombinant DNA, Gene, DNA, Biotechnology
Abstract

We found and characterized two cry8 genes from the Bacillus thuringiensis strain INTA Fr7-4 isolated in Argentina. These genes, cry8Kb3 and cry8Pa3, are located in a tandem array within a 13,200-bp DNA segment sequenced from a preparation of total DNA. They encode 1,169- and 1,176-amino-acid proteins, respectively. Both genes were cloned with their promoter sequences and the proteins were expressed separately in an acrystalliferous strain of B. thuringiensis leading to the formation of ovoid crystals in the recombinant strains. The toxicity against larvae of Anthonomus grandis Bh. (Coleoptera: Curculionidae) of a spore-crystal suspension from the recombinant strain containing cry8Pa3 was similar to that of the parent strain INTA Fr7-4.

Published
2014

4. Draft Genome Sequence of Geobacillus sp. Isolate T6, a Thermophilic Bacterium Collected from a Thermal Spring in Argentina

Author

Graciela Beatriz Benintende, Marcelo Facundo Berretta, Laura Emilce Navas, Elio Maximiliano Ortiz, Ariel Fernando Amadio, and Rubén O. Zandomeni

Subjects
Whole genome sequencing, biology, Thermophile, Genetics, Prokaryotes, Spring (mathematics), biology.organism_classification, Geobacillus sp, Molecular Biology, Gene, Bacteria, Microbiology
Abstract

Geobacillus sp. isolate T6 was collected from a thermal spring in Salta, Argentina. The draft genome sequence (3,767,773 bp) of this isolate is represented by one major scaffold of 3,46 Mbp, a second one of 207 kbp, and 20 scaffolds of

Published
2015

5. Draft Genome Sequence of Thermus sp. Isolate 2.9, Obtained from a Hot Water Spring Located in Salta, Argentina

Author

Marcelo Facundo Berretta, Graciela Beatriz Benintende, Laura Emilce Navas, Elio Maximiliano Ortiz, Ariel Alberto Amadío, and Rubén O. Zandomeni

Subjects
0106 biological sciences, Whole genome sequencing, 0303 health sciences, geography, geography.geographical_feature_category, Biology, 01 natural sciences, Thermus sp, 03 medical and health sciences, 010608 biotechnology, Spring (hydrology), Botany, Genetics, bacteria, Prokaryotes, Molecular Biology, 030304 developmental biology
Abstract

Thermus sp. isolate 2.9 was obtained from a hot water spring in Salta, Argentina. Here, we report the draft genome sequence (2,485,434 bp) of this isolate, which consists of 11 scaffolds of >10 kbp and 2,719 protein-coding sequences.

Published
2015

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