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Complete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes
- Source :
- Microbial Physiology. 27:43-54
- Publication Year :
- 2017
- Publisher :
- S. Karger AG, 2017.
-
Abstract
- We report the complete sequence and analysis of pFR260, a novel megaplasmid of 260,595 bp from the Bacillus thuringiensis strain INTA Fr7-4 isolated in Argentina. It carries 7 insecticidal genes: 3 cry8 copies previously reported, 2 vip1, and 2 vip2. Also, it carries a gene encoding a putative atypical Cry protein. These genes are arranged in a region of approximately 105 kbp in size with characteristics of a pathogenicity island with a potential coleopteran-specific insecticide profile. DNA strand composition asymmetry, as determined by GC skew analysis, and the presence of a Rep protein involved in the initiation of replication suggest a bidirectional theta mechanism of replication. In addition, many genes involved in conjugation and a CRISPR-Cas system were detected. The pFR260 sequence was deposited in GenBank under accession number KX258624.
Details
- ISSN :
- 26731673 and 26731665
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- Microbial Physiology
- Accession number :
- edsair.doi...........20c962586eb51a2cecd80d4bdcc479e9