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1. Analysis of seven SARS-CoV-2 rapid antigen tests in detecting omicron (B.1.1.529) versus delta (B.1.617.2) using cell culture supernatants and clinical specimens

Author

Jungnick, Sabrina, Hobmaier, Bernhard, Paravinja, Natali, Mautner, Lena, Hoyos, Mona, Konrad, Regina, Haase, Maren, Baiker, Armin, Eberle, Ute, Bichler, Magdalena, Treis, Bianca, Okeyo, Mercy, Streibl, Barbara, Wimmer, Clara, Hepner, Sabrina, Sprenger, Annika, Berger, Carola, Weise, Laura, Dangel, Alexandra, Ippisch, Siegfried, Jonas, Walter, Wildner, Manfred, Liebl, Bernhard, Ackermann, Nikolaus, Sing, Andreas, and Fingerle, Volker

Published
2023
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2. Molecular SARS-CoV-2 surveillance in Bavaria shows no Omicron transmission before the end of November 2021

Author

Flechsler, Jennifer, Eberle, Ute, Dangel, Alexandra, Hepner, Sabrina, Wimmer, Clara, Lutmayr, Johannes, Konrad, Regina, Berger, Carola, Weise, Laura, Sprenger, Annika, Zeitler, Jörg, Paravinja, Natali, Angermeier, Hildegard, Githure, George, Schmidt, Sandra, Treis, Bianca, Okeyo, Mercy, Liebl, Bernhard, Ackermann, Nikolaus, and Sing, Andreas

Published
2022
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6. Weekly SARS-CoV-2 Sentinel Surveillance in Primary Schools, Kindergartens, and Nurseries, Germany, June-November 2020

Author

Hoch, Martin, Vogel, Sebastian, Kolberg, Laura, Dick, Elisabeth, Fingerle, Volker, Eberle, Ute, Ackermann, Nikolaus, Sing, Andreas, Huebner, Johannes, Rack-Hoch, Anita, Schober, Tilmann, and von Both, Ulrich

Subjects
Epidemics -- Statistics -- Risk factors -- Control, Sentinel health events -- Methods, Elementary schools -- Statistics -- Health aspects -- Safety and security measures -- Germany, Nursery schools -- Statistics -- Health aspects -- Safety and security measures, Health
Abstract

We investigated severe acute respiratory syndrome coronavirus 2 infections in primary schools, kindergartens, and nurseries in Germany. Of 3,169 oropharyngeal swab specimens, only 2 were positive by real-time reverse transcription [...]

Published
2021
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8. Treponemen

Author

Berger, Anja, Eberle, Ute, Ackermann, Nikolaus, Fingerle, Volker, Sing, Andreas, Suerbaum, Sebastian, editor, Burchard, Gerd-Dieter, editor, Kaufmann, Stefan H. E., editor, and Schulz, Thomas F., editor

Published
2020
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9. Investigation of a COVID-19 outbreak in Germany resulting from a single travel-associated primary case: a case series

Author

Böhmer, Merle M, Buchholz, Udo, Corman, Victor M, Hoch, Martin, Katz, Katharina, Marosevic, Durdica V, Böhm, Stefanie, Woudenberg, Tom, Ackermann, Nikolaus, Konrad, Regina, Eberle, Ute, Treis, Bianca, Dangel, Alexandra, Bengs, Katja, Fingerle, Volker, Berger, Anja, Hörmansdorfer, Stefan, Ippisch, Siegfried, Wicklein, Bernd, Grahl, Andreas, Pörtner, Kirsten, Muller, Nadine, Zeitlmann, Nadine, Boender, T Sonia, Cai, Wei, Reich, Andreas, an der Heiden, Maria, Rexroth, Ute, Hamouda, Osamah, Schneider, Julia, Veith, Talitha, Mühlemann, Barbara, Wölfel, Roman, Antwerpen, Markus, Walter, Mathias, Protzer, Ulrike, Liebl, Bernhard, Haas, Walter, Sing, Andreas, Drosten, Christian, and Zapf, Andreas

Published
2020
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10. 10 Must Knows from Biodiversity Science 2024

Author

Leibniz Research Network Biodiversity., Thonicke, Kirsten; https://orcid.org/0000-0001-5283-4937, Rahner, Eva, Arneth, Almut, Bonn, Aletta, Borchard, Nils, Chaudhary, Abishek, Darbi, Marianne, Dutta, Trishna, Eberle, Ute, Eisenhauer, Nico, Farwig, Nina, Flocco, Cecilia G, Freitag, Jens, Grobe, Peter, Grosch, Rita, Grossart, Hans Peter, Grosse, Angela, Grützmacher, Kim, Hagemann, Nina, Hansjürgens, Bernd, Hartmann Scholz, Amber, Hassenrück, Christiane, Häuser, Christoph, Hickler, Thomas, Hölker, Franz; https://orcid.org/0000-0001-5932-266X, Jacob, Ute, Jähnig, Sonja C; https://orcid.org/0000-0002-6349-9561, Jürgens, Klaus, Krug, Cornelia; https://orcid.org/0000-0002-2470-1229, et al, Leibniz Research Network Biodiversity., Thonicke, Kirsten; https://orcid.org/0000-0001-5283-4937, Rahner, Eva, Arneth, Almut, Bonn, Aletta, Borchard, Nils, Chaudhary, Abishek, Darbi, Marianne, Dutta, Trishna, Eberle, Ute, Eisenhauer, Nico, Farwig, Nina, Flocco, Cecilia G, Freitag, Jens, Grobe, Peter, Grosch, Rita, Grossart, Hans Peter, Grosse, Angela, Grützmacher, Kim, Hagemann, Nina, Hansjürgens, Bernd, Hartmann Scholz, Amber, Hassenrück, Christiane, Häuser, Christoph, Hickler, Thomas, Hölker, Franz; https://orcid.org/0000-0001-5932-266X, Jacob, Ute, Jähnig, Sonja C; https://orcid.org/0000-0002-6349-9561, Jürgens, Klaus, Krug, Cornelia; https://orcid.org/0000-0002-2470-1229, and et al

Published
2024

16. The representative COVID-19 cohort Munich (KoCo19): from the beginning of the pandemic to the Delta virus variant

Author

Le Gleut, Ronan, Plank, Michael, Pütz, Peter, Radon, Katja, Bakuli, Abhishek, Rubio-Acero, Raquel, Paunovic, Ivana, Rieß, Friedrich, Winter, Simon, Reinkemeyer, Christina, Schälte, Yannik, Olbrich, Laura, Hannes, Marlene, Kroidl, Inge, Noreña, Ivan, Janke, Christian, Wieser, Andreas, Hoelscher, Michael, Fuchs, Christiane, Castelletti, Noemi, Ahmed, Mohamed Ibraheem Mohamed, Alamoudi, Emad, Anderson, Jared, Baldassarre, Valeria, Baumann, Maximilian, Becker, Marc, Bednarski, Franziska, Behlen, Marieke, Bemirayev, Olimbek, Beyerl, Jessica, Bitzer, Patrick, Böhnlein, Rebecca, Brand, Isabel, Brauer, Anna, Britz, Vera, Bruger, Jan, Bünz, Franziska, Caroli, Friedrich, Coleman, Josephine, Contento, Lorenzo, Czwienzek, Alina, Deák, Flora, Diefenbach, Maximilian N., Diepers, Paulina, Do, Anna, Dobler, Gerhard, Durner, Jürgen, Eser, Tabea, Eberle, Ute, Eckstein, Judith, Falk, Philine, Feyereisen, Manuela, Fingerle, Volker, Fischer, Stefanie, Frese, Jonathan, Forster, Felix, Fröschl, Günter, Geisenberger, Otto, Garí, Mercè, Gasser, Marius, Gauder, Sonja, Geier, Raffaela, Gillig, Kristina, Geldmacher, Christof, Gezgin, Keisha, Gilberg, Leonard, Girl, Philipp, Golschan, Elias, Grauvogl, Vitus, Noller, Jessica Michelle Guggenbuehl, Guglielmini, Elena Maria, Gutierrez, Pablo, Haderer, Anselm, Halfmann, Celina, Hartinger, Lena, Haselwarter, Timm, Hasenauer, Jan, Hernandez, Alejandra, Heller, Luca, Heiber, Arlett, Herrmann, Matthias, Hillari, Leah, Hillmann, Stefan, Hinske, Christian, Hoefflin, Janna, Hofberger, Tim, Höfinger, Michael, Hofmann, Larissa, Horn, Sacha, Huber, Kristina, Karger, Lilian, Kappl, Ursula, Keßler, Antonia, Khan, Zohaib, Kiani, Charlotte, Klugherz, Isabel, Kreider, Norah, Kresin, Johanna, Kroidl, Arne, Kunder, Pratik, Lang, Magdalena, Lang, Clemens, Lange, Silvan, Lapteva, Ekaterina, Laxy, Michael, Leidl, Reiner, Liedl, Leopold, Lindner, Felix, Lucaj, Xhovana, Lucke, Elisabeth, Luppa, Fabian, Nafziger, Alexandra Sophie, Maczka, Alexander, Mang, Petra, Markgraf, Alisa, Matcau, Paula, Mayrhofer, Rebecca, Mekota, Anna-Maria, Metaxa, Dafni, Mohr, Emily, Müller, Hannah, Müller, Katharina, Nascimento, Nathalia, Niermeyer, Kasimir, Nikolaides, Sophia, Pattard, Leonie, Pleimelding, Claire, Pletschette, Michel, Poll, Viona, Prückner, Stephan, Puchinger, Kerstin, Pusl, Konstantin, Raimúndez, Elba, Raschka, Julius, Reich, Jakob, Rothe, Camilla, Ruci, Viktoria, Saathoff, Elmar, Schäfer, Nicole, Schandelmaier, Paul, Schluse, Benedikt, Schneider, Annika, Schneider, Lara, Schultz, Sophie, Schunk, Mirjam, Schwettmann, Lars, Sedlmeier, Josefin, Sintu-Sempta, Linda, Soler, Alba, Sothmann, Peter, Strobl, Katharina, Strüber, Aida, Strüber, Laura, Tang, Jeni, Theis, Fabian, Thiel, Verena, Thumser, Eva, Thur, Niklas, Ullrich, Julian, Vollmayr, Vincent, Von Lovenberg, Emilia, Von Lovenberg, Jonathan, Vos, Carsten, Waibel, Julia, Wallrauch, Claudia, Weigl, Nikolas, Wölfl, Roman, Wolff, Julia, Wullinger, Pia, Würfel, Tobias, Wustrow, Patrick, Zange, Sabine, Zeggini, Eleftheria, Zielke, Anna, Zimmer, Thorbjörn, Zimmermann, Thomas, and Zuche, Lea

Abstract

**Background** Population-based serological studies allow to estimate prevalence of SARS-CoV-2 infections despite a substantial number of mild or asymptomatic disease courses. This became even more relevant for decision making after vaccination started. The KoCo19 cohort tracks the pandemic progress in the Munich general population for over two years, setting it apart in Europe. **Methods** Recruitment occurred during the initial pandemic wave, including 5313 participants above 13 years from private households in Munich. Four follow-ups were held at crucial times of the pandemic, with response rates of at least 70%. Participants filled questionnaires on socio-demographics and potential risk factors of infection. From Follow-up 2, information on SARS-CoV-2 vaccination was added. SARS-CoV-2 antibody status was measured using the Roche Elecsys® Anti-SARS-CoV-2 anti-N assay (indicating previous infection) and the Roche Elecsys® Anti-SARS-CoV-2 anti-S assay (indicating previous infection and/or vaccination). This allowed us to distinguish between sources of acquired antibodies. **Results** The SARS-CoV-2 estimated cumulative sero-prevalence increased from 1.6% (1.1-2.1%) in May 2020 to 14.5% (12.7-16.2%) in November 2021. Underreporting with respect to official numbers fluctuated with testing policies and capacities, becoming a factor of more than two during the second half of 2021. Simultaneously, the vaccination campaign against the SARS-CoV-2 virus increased the percentage of the Munich population having antibodies, with 86.8% (85.5-87.9%) having developed anti-S and/or anti-N in November 2021. Incidence rates for infections after (BTI) and without previous vaccination (INS) differed (ratio INS/BTI of 2.1, 0.7-3.6). However, the prevalence of infections was higher in the non-vaccinated population than in the vaccinated one. Considering the whole follow-up time, being born outside Germany, working in a high-risk job and living area per inhabitant were identified as risk factors for infection, while other socio-demographic and health-related variables were not. Although we obtained significant within-household clustering of SARS-CoV-2 cases, no further geospatial clustering was found. **Conclusions** Vaccination increased the coverage of the Munich population presenting SARS-CoV-2 antibodies, but breakthrough infections contribute to community spread. As underreporting stays relevant over time, infections can go undetected, so non-pharmaceutical measures are crucial, particularly for highly contagious strains like Omicron.

Published
2023
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18. Geht in Kentucky das Holz fürs Feuerwasser aus?

Author

Eberle, Ute

Subjects
WOOD, WHITE oak, WHISKEY industry, CHEMICAL reactions, WHISKEY
Abstract

Copyright of brand eins is the property of brand eins Medien AG and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2023

19. Epidemiological and Serological Analysis of a SARS-CoV-2 Outbreak in a Nursing Home: Impact of SARS-CoV-2 Vaccination and Enhanced Neutralizing Immunity Following Breakthrough Infection

Author

Streibl, Barbara I., primary, Lahne, Heidi, additional, Grahl, Andreas, additional, Agsten, Philipp, additional, Bichler, Magdalena, additional, Büchl, Christa, additional, Damzog, Marco, additional, Eberle, Ute, additional, Gärtner, Stefan, additional, Hobmaier, Bernhard, additional, Margos, Gabriele, additional, Hoch, Martin, additional, Jungnick, Sabrina, additional, Jonas, Walter, additional, Katz, Katharina, additional, Laubert, Liane, additional, Schutt, Barbara, additional, Seidl, Cornelia, additional, Treis, Bianca, additional, Weindl, Daniel, additional, Zilch, Karen, additional, Wildner, Manfred, additional, Liebl, Bernhard, additional, Ackermann, Nikolaus, additional, Sing, Andreas, additional, and Fingerle, Volker, additional

Published
2022
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20. The interplay of viral loads, clinical presentation, and serological responses in SARS-CoV-2 – Results from a prospective cohort of outpatient COVID-19 cases

Author

Puchinger, Kerstin, primary, Castelletti, Noemi, additional, Rubio-Acero, Raquel, additional, Geldmacher, Christof, additional, Eser, Tabea M., additional, Deák, Flora, additional, Paunovic, Ivana, additional, Bakuli, Abhishek, additional, Saathoff, Elmar, additional, von Meyer, Alexander, additional, Markgraf, Alisa, additional, Falk, Philine, additional, Reich, Jakob, additional, Riess, Friedrich, additional, Girl, Philipp, additional, Müller, Katharina, additional, Radon, Katja, additional, Guggenbuehl Noller, Jessica Michelle, additional, Wölfel, Roman, additional, Hoelscher, Michael, additional, Kroidl, Inge, additional, Wieser, Andreas, additional, Olbrich, Laura, additional, Alamoudi, Emad, additional, Anderson, Jared, additional, Baumann, Maximilian, additional, Behlen, Marieke, additional, Beyerl, Jessica, additional, Böhnlein, Rebecca, additional, Brauer, Anna, additional, Britz, Vera, additional, Bruger, Jan, additional, Caroli, Friedrich, additional, Contento, Lorenzo, additional, Diekmannshemke, Jana, additional, Do, Anna, additional, Dobler, Gerhard, additional, Eberle, Ute, additional, Eckstein, Judith, additional, Frese, Jonathan, additional, Forster, Felix, additional, Frahnow, Turid, additional, Fröschl, Günter, additional, Geisenberger, Otto, additional, Gillig, Kristina, additional, Heiber, Arlett, additional, Hinske, Christian, additional, Hoefflin, Janna, additional, Hofberger, Tim, additional, Höfinger, Michael, additional, Hofmann, Larissa, additional, Horn, Sacha, additional, Huber, Kristina, additional, Janke, Christian, additional, Kappl, Ursula, additional, Kiani, Charlotte, additional, Kroidl, Arne, additional, Laxy, Michael, additional, Leidl, Reiner, additional, Lindner, Felix, additional, Mayrhofer, Rebecca, additional, Mekota, Anna-Maria, additional, Müller, Hannah, additional, Metaxa, Dafni, additional, Pattard, Leonie, additional, Pletschette, Michel, additional, Prückner, Stephan, additional, Pusl, Konstantin, additional, Raimúndez, Elba, additional, Rothe, Camila, additional, Schäfer, Nicole, additional, Schandelmaier, Paul, additional, Schneider, Lara, additional, Schultz, Sophie, additional, Schunk, Mirjam, additional, Schwettmann, Lars, additional, Seibold, Heidi, additional, Sothmann, Peter, additional, Stapor, Paul, additional, Theis, Fabian, additional, Thiel, Verena, additional, Thiesbrummel, Sophie, additional, Thur, Niklas, additional, Waibel, Julia, additional, Wallrauch, Claudia, additional, Winter, Simon, additional, Wolff, Julia, additional, Wullinger, Pia, additional, Yaqine, Houda, additional, Zange, Sabine, additional, Zeggini, Eleftheria, additional, Zimmermann, Thomas, additional, Zielke, Anna, additional, Ibraheem, Mohamed, additional, Ahmed, Mohamed, additional, Becker, Marc, additional, Diepers, Paulina, additional, Schälte, Yannik, additional, Garí, Mercè, additional, Pütz, Peter, additional, Pritsch, Michael, additional, Fingerle, Volker, additional, Le Gleut, Ronan, additional, Gilberg, Leonard, additional, Brand, Isabel, additional, Diefenbach, Max, additional, Eser, Tabea, additional, Weinauer, Franz, additional, Martin, Silke, additional, Quenzel, Ernst-Markus, additional, Durner, Jürgen, additional, Fuchs, Christiane, additional, and Hasenauer, Jan, additional

Published
2022
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22. Reciprocal circulation pattern of SARS-CoV-2 and influenza viruses during the influenza seasons 2019/2020 and 2020/2021 in the Bavarian Influenza Sentinel (Germany) – CORRIGENDUM

Author

Heinzinger, Susanne, primary, Eberle, Ute, additional, Angermeier, Hildegard, additional, Flechsler, Jennifer, additional, Konrad, Regina, additional, Dangel, Alexandra, additional, Berger, Carola, additional, Sprenger, Annika, additional, Hepner, Sabrina, additional, Biere, Barbara, additional, Liebl, Bernhard, additional, Ackermann, Nikolaus, additional, and Sing, Andreas, additional

Published
2022
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23. Feasibility and Diagnostic Accuracy of Saliva-Based SARS-CoV-2 Screening in Educational Settings and Children Aged &lt

Author

Hoch, Martin, Vogel, Sebastian, Eberle, Ute, Kolberg, Laura, Gruenthaler, Valerie, Fingerle, Volker, Ackermann, Nikolaus, Sing, Andreas, Liebl, Bernhard, Huebner, Johannes, Kuttiadan, Simone, Rack-Hoch, Anita, Meyer-Buehn, Melanie, Schober, Tilmann, and Both, Ulrich von

Subjects
saliva sampling, SARS-CoV-2, childcare facilities, Salivette®, primary school
Abstract

Children have been disproportionately affected during the COVID-19 pandemic. We aimed to assess a saliva-based algorithm for SARS-CoV-2 testing to be used in schools and childcare institutions under pandemic conditions. A weekly SARS-CoV-2 sentinel study in primary schools, kindergartens, and childcare facilities was conducted over a 12-week-period. In a sub-study covering 7 weeks, 1895 paired oropharyngeal and saliva samples were processed for SARS-CoV-2 rRT-PCR testing in both asymptomatic children (n = 1243) and staff (n = 652). Forty-nine additional concurrent swab and saliva samples were collected from SARS-CoV-2 infected patients (patient cohort). The Salivette® system was used for saliva collection and assessed for feasibility and diagnostic performance. For children, a mean of 1.18 mL saliva could be obtained. Based on results from both cohorts, the Salivette® testing algorithm demonstrated the specificity of 100% (95% CI 99.7–100) and sensitivity of 94.9% (95% CI 81.4–99.1) with oropharyngeal swabs as reference. Agreement between sampling systems was 100% for moderate to high viral load situations (defined as Ct-values &lt, 33 from oropharyngeal swabs). Comparative analysis of Ct-values derived from saliva vs. oropharyngeal swabs demonstrated a significant difference (mean 4.23, 95% CI 2.48–6.00). In conclusion, the Salivette® system proved to be an easy-to-use, safe and feasible saliva collection method and a more pleasant alternative to oropharyngeal swabs for SARS-CoV-2 testing in children aged 3 years and above.

Published
2021
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24. Feasibility and Diagnostic Accuracy of Saliva-Based SARS-CoV-2 Screening in Educational Settings and Children Aged <12 Years

Author

Hoch, Martin, primary, Vogel, Sebastian, additional, Eberle, Ute, additional, Kolberg, Laura, additional, Gruenthaler, Valerie, additional, Fingerle, Volker, additional, Ackermann, Nikolaus, additional, Sing, Andreas, additional, Liebl, Bernhard, additional, Huebner, Johannes, additional, Kuttiadan, Simone, additional, Rack-Hoch, Anita, additional, Meyer-Buehn, Melanie, additional, Schober, Tilmann, additional, and von Both, Ulrich, additional

Published
2021
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25. In Vitro Rapid Antigen Test Performance with the SARS-CoV-2 Variants of Concern B.1.1.7 (Alpha), B.1.351 (Beta), P.1 (Gamma), and B.1.617.2 (Delta)

Author

Jungnick, Sabrina, primary, Hobmaier, Bernhard, additional, Mautner, Lena, additional, Hoyos, Mona, additional, Haase, Maren, additional, Baiker, Armin, additional, Lahne, Heidi, additional, Eberle, Ute, additional, Wimmer, Clara, additional, Hepner, Sabrina, additional, Sprenger, Annika, additional, Berger, Carola, additional, Dangel, Alexandra, additional, Ippisch, Siegfried, additional, Hahner, Sonja, additional, Wildner, Manfred, additional, Liebl, Bernhard, additional, Ackermann, Nikolaus, additional, Sing, Andreas, additional, and Fingerle, Volker, additional

Published
2021
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26. Spatially resolved qualified sewage spot sampling to track SARS-CoV-2 dynamics in Munich - One year of experience

Author

Raquel Rubio-Acero, Jessica Beyerl, Maximilian Muenchhoff, Marc Sancho Roth, Noemi Castelletti, Ivana Paunovic, Katja Radon, Bernd Springer, Christian Nagel, Bernhard Boehm, Merle M. Böhmer, Alexander Graf, Helmut Blum, Stefan Krebs, Oliver T. Keppler, Andreas Osterman, Zohaib Nisar Khan, Michael Hoelscher, Andreas Wieser, Alamoudi Emad, Anderson Jared, Bakuli Abhishek, Baumann Maxilmilian, Becker Marc, Bednarzki Franziska, Bemirayev Olimbek, Beyerl Jessica, Bitzer Patrick, Böhnlein Rebecca, Brand Isabel, Bruger Jan, Caroli Friedrich, Castelletti Noemi, Coleman Josephine, Contento Lorenzo, Czwienzek Alina, Deák Flora, N. Diefenbach Maximilian, Diekmannshemke Jana, Dobler Gerhard, Durner Jürgen, Eberle Ute, Eckstein Judith, Eser Tabea, Falk Philine, Feyereisen Manuela, Fingerle Volker, Forster Felix, Frahnow Turid, Frese Jonathan, Fröschl Günter, Fuchs Christiane, Garí Mercè, Geisenberger Otto, Geldmacher Christof, Gilberg Leonard, Gillig Kristina, Girl Philipp, Golschan Elias, Guggenbuehl Noller Jessica Michelle, Guglielmini Elena Maria, Gutierrez Pablo, Haderer Anslem, Hannes Marlene, Hartinger Lena, Hasenauer Jan, Hernandez Alejandra, Hillari Leah, Hinske Christian, Hofberger Tim, Hölscher Michael, Horn Sacha, Huber Kristina, Janke Christian, Kappl Ursula, Keßler Antonia, Khan Zohaib, Kresin Johanna, Kroidl Inge, Kroidl Arne, Lang Magdalena, Lang Clemens, Lange Silvan, Laxy Michael, Le Gleut Ronan, Leidl Reiner, Liedl Leopold, Lucaj Xhovana, Luppa Fabian, Nafziger Alexandra Sophie, Mang Petra, Markgraf Alisa, Mayrhofer Rebecca, Metaxa Dafni, Müller Hannah, Müller Katharina, Olbrich Laura, Paunovic Ivana, Plank Michael, Pleimelding Claire, Pletschette Michel, Pritsch Michael, Prückner Stephan, Puchinger Kerstin, Pütz Peter, Radon Katja, Raimundéz Elba, Reich Jakob, Riess Friedrich, Rothe Camilla, Rubio-Acero Raquel, Ruci Viktoria, Saathoff Elmar, Schäfer Nicole, Schälte Yannik, Schluse Benedikt, Schneider Lara, Schunk Mirjam, Schwettmann Lars, Soler Alba, Sothmann Peter, Strobl Kathrin, Tang Jeni, Theis Fabian, Thiel Verena, Thiesbrummel Sophie, Vollmayr Vincent, Von Lovenberg Emilia, Von Lovenberg Jonathan, Waibel Julia, Wallrauch Claudia, Wieser Andreas, Winter Simon, Wölfel Roman, Wolff Julia, Würfel Tobias, Zange Sabine, Zeggini Eleftheria, Zielke Anna, and Zimmer Thorbjörn

Subjects
Environmental Engineering, Population, Sewage, Wastewater, Track (rail transport), Article, Pandemic, Environmental Chemistry, Humans, Sequencing, education, B.1.1.7, Waste Management and Disposal, education.field_of_study, Surveillance, business.industry, SARS-CoV-2, Incidence (epidemiology), Sampling (statistics), COVID-19, Pollution, Metropolitan area, Geography, PCR, RNA, Viral, business, Cartography, Viral load
Abstract

Wastewater-based epidemiology (WBE) is a tool now increasingly proposed to monitor the SARS-CoV-2 burden in populations without the need for individual mass testing. It is especially interesting in metropolitan areas where spread can be very fast, and proper sewage systems are available for sampling with short flow times and thus little decay of the virus. We started in March 2020 to set up a once-a-week qualified spot sampling protocol in six different locations in Munich carefully chosen to contain primarily wastewater of permanent residential areas, rather than industry or hospitals. We used RT-PCR and sequencing to track the spread of SARS-CoV-2 in the Munich population with temporo-spatial resolution. The study became fully operational in mid-April 2020 and has been tracking SARS-CoV-2 RNA load weekly for one year. Sequencing of the isolated viral RNA was performed to obtain information about the presence and abundance of variants of concern in the Munich area over time. We demonstrate that the evolution of SARS-CoV-2 RNA loads (between, Graphical abstract Unlabelled Image

Published
2021

27. Detection of the new SARS-CoV-2 variants of concern B.1.1.7 and B.1.351 in five SARS-CoV-2 rapid antigen tests (RATs), Germany, March 2021

Author

Jungnick, Sabrina, Hobmaier, Bernhard, Mautner, Lena, Hoyos, Mona, Haase, Maren, Baiker, Armin, Lahne, Heidi, Eberle, Ute, Wimmer, Clara, Hepner, Sabrina, Sprenger, Annika, Berger, Carola, Dangel, Alexandra, Wildner, Manfred, Liebl, Bernhard, Ackermann, Nikolaus, Sing, Andreas, Fingerle, Volker, Bartha-Dima, Bernadett, Bengs, Katja, Berger, Anja, Boll, Kerstin, Carl, Anja, Jürgen, Christian, Drdlicek, Juliana, Eisenberger, David, Flechsler, Jennifer, Gerdes, Lars, Githure, George, Govindaswamy, Janani, Hupfer, Christine, Konrad, Regina, Lupoli, Gaia, Lutmayr, Johannes, Margos, Gabriele, Müller, Roswitha, Nickel, Silke, Okeyo, Mercy, Pavlovic, Melanie, Pecoraro, Sven, Sahm, Isabel, Schauer, Melanie, Schülein, Anika, Schürmann, Eva-Maria, Schulze, Gesine, Scuda, Nelly, Singer, Stefanie, Stellberger, Thorsten, Treis, Bianca, Tuschak, Christian, Zimmermann, Pia, and Paravinja, Natali

Subjects
0301 basic medicine, 2019-20 coronavirus outbreak, Saliva, Strain (chemistry), Coronavirus disease 2019 (COVID-19), Serial dilution, Epidemiology, SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Public Health, Environmental and Occupational Health, Variant(s) of Concern, B.1.351, Biology, Virology, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Antigen, Cell culture, Rapid antigen test(s), 030212 general & internal medicine, B.1.1.7, Rapid Communication, performance
Abstract

SARS-CoV-2 variants of concern (VOC) should not escape molecular surveillance. We investigated if SARS-CoV-2 rapid antigen tests (RATs) could detect B.1.1.7 and B.1.351 VOCs in certain laboratory conditions. Infectious cell culture supernatants containing B.1.1.7, B.1.351 or non-VOC SARS-CoV-2 were respectively diluted both in DMEM and saliva. Dilutions were analysed with Roche, Siemens, Abbott, nal von minden and RapiGEN RATs. While further studies with appropriate real-life clinical samples are warranted, all RATs detected B.1.1.7 and B.1.351, generally comparable to non-VOC strain.

Published
2021

29. From first to second wave: follow-up of the prospective COVID-19 cohort (KoCo19) in Munich (Germany)

Author

Radon, Katja, Bakuli, Abhishek, Pütz, Peter, Le Gleut, Ronan, Guggenbuehl Noller, Jessica Michelle, Olbrich, Laura, Saathoff, Elmar, Garí, Mercè, Schälte, Yannik, Frahnow, Turid, Wölfel, Roman, Pritsch, Michael, Rothe, Camilla, Pletschette, Michel, Rubio-Acero, Raquel, Beyerl, Jessica, Metaxa, Dafni, Forster, Felix, Thiel, Verena, Castelletti, Noemi, Rieß, Friedrich, Diefenbach, Maximilian N., Fröschl, Günter, Bruger, Jan, Winter, Simon, Frese, Jonathan, Puchinger, Kerstin, Brand, Isabel, Kroidl, Inge, Wieser, Andreas, Hoelscher, Michael, Hasenauer, Jan, Fuchs, Christiane, Ackermann, Nikolaus, Alamoudi, Emad, Anderson, Jared, Baumann, Maxilmilian, Becker, Marc, Bednarzki, Franziska, Bemirayev, Olimbek, Bitzer, Patrick, Böhnlein, Rebecca, Caroli, Friedrich, Coleman, Josephine, Contento, Lorenzo, Czwienzek, Alina, Deák, Flora, Diekmannshemke, Jana, Dobler, Gerhard, Durner, Jürgen, Eberle, Ute, Eckstein, Judith, Eser, Tabea, Falk, Philine, Feyereisen, Manuela, Fingerle, Volker, Geisenberger, Otto, Geldmacher, Christof, Gilberg, Leonard, Gillig, Kristina, Girl, Philipp, Golschan, Elias, Guglielmini, Elena Maria, Gutierrez, Pablo, Haderer, Anslem, Hannes, Marlene, Hartinger, Lena, Hernandez, Alejandra, Hillari, Leah, Hinske, Christian, Hofberger, Tim, Horn, Sacha, Huber, Kristina, Janke, Christian, Kappl, Ursula, Keßler, Antonia, Khan, Zohaib, Kresin, Johanna, Kroidl, Arne, Lang, Magdalena, Lang, Clemens, Lange, Silvan, Laxy, Michael, Leidl, Reiner, Liedl, Leopold, Lucaj, Xhovana, Luppa, Fabian, Nafziger, Alexandra Sophie, Mang, Petra, Markgraf, Alisa, Mayrhofer, Rebecca, Müller, Hannah, Müller, Katharina, Paunovic, Ivana, Plank, Michael, Pleimelding, Claire, Prückner, Stephan, Raimúndez, Elba, Reich, Jakob, Ruci, Viktoria, Schäfer, Nicole, Schluse, Benedikt, Schneider, Lara, Schunk, Mirjam, Schwettmann, Lars, Sing, Andreas, Soler, Alba, Sothmann, Peter, Strobl, Kathrin, Tang, Jeni, Theis, Fabian, Thiesbrummel, Sophie, Vollmayr, Vincent, von Lovenberg, Emilia, von Lovenberg, Jonathan, Waibel, Julia, Wallrauch, Claudia, Wolff, Julia, Würfel, Tobias, Yaqine, Houda, Zange, Sabine, Zeggini, Eleftheria, Zielke, Anna, and Zimmer, Thorbjörn

Subjects
Research Article, Viral diseases, COVID-19, SARS-CoV-2, Population-based cohort study, Sero-prevalence, Sero-incidence, ORCHESTRA, ddc
Published
2020

30. Feasibility and diagnostic accuracy of saliva-based SARS-CoV-2 screening in educational settings and children aged < 12 years

Author

Hoch, Martin, primary, Vogel, Sebastian, additional, Eberle, Ute, additional, Kolberg, Laura, additional, Gruenthaler, Valerie, additional, Fingerle, Volker, additional, Ackermann, Nikolaus, additional, Sing, Andreas, additional, Liebl, Bernhard, additional, Huebner, Johannes, additional, Kuttiadan, Simone, additional, Rack-Hoch, Anita, additional, Meyer-Buehn, Melanie, additional, Schober, Tilmann, additional, and Both, Ulrich von, additional

Published
2021
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33. Weekly SARS-CoV-2 sentinel in primary schools, kindergartens and nurseries, June to November 2020, Germany

Author

Hoch, Martin, primary, Vogel, Sebastian, additional, Kolberg, Laura, additional, Dick, Elisabeth, additional, Fingerle, Volker, additional, Eberle, Ute, additional, Ackermann, Nikolaus, additional, Sing, Andreas, additional, Huebner, Johannes, additional, Rack-Hoch, Anita, additional, Schober, Tilmann, additional, and Both, Ulrich von, additional

Published
2021
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34. Reciprocal circulation pattern of SARS-CoV-2 and influenza viruses during the influenza seasons 2019/2020 and 2020/2021 in the Bavarian Influenza Sentinel (Germany)

Author

Heinzinger, Susanne, primary, Eberle, Ute, additional, Angermeier, Hildegard, additional, Flechsler, Jennifer, additional, Konrad, Regina, additional, Dangel, Alexandra, additional, Berger, Carola, additional, Sprenger, Annika, additional, Hepner, Sabrina, additional, Biere, Barbara, additional, Liebl, Bernhard, additional, Ackermann, Nikolaus, additional, and Sing, Andreas, additional

Published
2021
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37. Additional file 4 of Rapid point-of-care detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Author

Mautner, Lena, Christin-Kirsty Baillie, Herold, Heike Marie, Volkwein, Wolfram, Guertler, Patrick, Eberle, Ute, Ackermann, Nikolaus, Sing, Andreas, Pavlovic, Melanie, Goerlich, Ottmar, Busch, Ulrich, Wassill, Lars, Huber, Ingrid, and Baiker, Armin

Abstract

Additional file 4: Figure S3. Specificity of the assay. SARS-CoV Frankfurt 1 RNA and nucleic acid extracts from 20 different samples from respiratory pathogens were tested with RT-LAMP targeting ORF8 (A) and N (B) to determine cross reactivity. For non-template control (NTC) PCR grade water was used.

Published
2020
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38. Additional file 2 of Rapid point-of-care detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Author

Mautner, Lena, Christin-Kirsty Baillie, Herold, Heike Marie, Volkwein, Wolfram, Guertler, Patrick, Eberle, Ute, Ackermann, Nikolaus, Sing, Andreas, Pavlovic, Melanie, Goerlich, Ottmar, Busch, Ulrich, Wassill, Lars, Huber, Ingrid, and Baiker, Armin

Subjects
stomatognathic system
Abstract

Additional file 2: Table S1. Seven different swab types for suitability test of different swab types for RT-LAMP.

Published
2020
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39. Additional file 1 of Rapid point-of-care detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Author

Mautner, Lena, Christin-Kirsty Baillie, Herold, Heike Marie, Volkwein, Wolfram, Guertler, Patrick, Eberle, Ute, Ackermann, Nikolaus, Sing, Andreas, Pavlovic, Melanie, Goerlich, Ottmar, Busch, Ulrich, Wassill, Lars, Huber, Ingrid, and Baiker, Armin

Subjects
genetic structures, sense organs, eye diseases
Abstract

Additional file 1: Figure S1. Positions of LAMP primers in SARS-CoV-2 genome. LAMP primers of genes ORF8 (A) and N (B) aligned to the SARS-CoV-2 reference sequence. Arrowheads indicate orientation of the primer. Primers F1c and F2 together build the FIP primer, whereas primers B1c and B2 together build the BIP primer of the different LAMP primer sets.

Published
2020
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40. Additional file 5 of Rapid point-of-care detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Author

Mautner, Lena, Christin-Kirsty Baillie, Herold, Heike Marie, Volkwein, Wolfram, Guertler, Patrick, Eberle, Ute, Ackermann, Nikolaus, Sing, Andreas, Pavlovic, Melanie, Goerlich, Ottmar, Busch, Ulrich, Wassill, Lars, Huber, Ingrid, and Baiker, Armin

Abstract

Additional file 5: Figure S4. Colorimetric read-out of SARS-CoV-2 RT-LAMP. Positive samples 1–5 and Negative samples 1–5 were pre-heated at 90 °C for 5 min before being directly pipetted into RT-LAMP for SARS-CoV-2 targeting ORF8 and N. As internal control (IC) RNase P was additionally targeted. WarmStart Colorimetric LAMP 2x Master Mix (#M1800S, NEB, Ipswich, USA) was used by the manufacturer’s instructions with 5 µL of heated swab sample. For non-template control (NTC) PCR grade water substituted swab sample. Pink colour indicates negative result, yellow signals positive test result.

Published
2020
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41. Additional file 3 of Rapid point-of-care detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Author

Mautner, Lena, Christin-Kirsty Baillie, Herold, Heike Marie, Volkwein, Wolfram, Guertler, Patrick, Eberle, Ute, Ackermann, Nikolaus, Sing, Andreas, Pavlovic, Melanie, Goerlich, Ottmar, Busch, Ulrich, Wassill, Lars, Huber, Ingrid, and Baiker, Armin

Abstract

Additional file 3: Figure S2. Determination of the sensitivity of the assay. Isolated RNA from SARS-CoV-2 infected Vero cell culture supernatant was quantified using RT-ddPCR and serially diluted to determine the LOD. 1000 copies/µL and 200 copies/µL dilutions were analysed in six replicates, while every lower dilution (1000–0.2 copies/µL) were analysed in 12 replicates. For both target genes ORF8 (A) and N (B) the LOD is 100 copies/µL as the last dilution where all 12 replicates are positive. For non-template control (NTC) PCR grade water substituted SARS-CoV-2 RNA.

Published
2020
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42. Detection and viral RNA shedding of SARS-CoV-2 in respiratory specimens relative to symptom onset among COVID-19 patients in Bavaria, Germany.

Author

Woudenberg, Tom, Eberle, Ute, Marosevic, Durdica, Liebl, Bernhard, Ackermann, Nikolaus, Katz, Katharina, Sing, Andreas, Bavarian SARS-CoV-2-Group, Baiker, Armin, Bartha-Dima, Bernadett, Bengs, Katja, Berger, Anja, Böhm, Stefanie, Böhmer, Merle, Boll, Kerstin, Carl, Anja, Christian, Jürgen, Dangel, Alexandra, Drdlicek, Juliana, and Eisenberger, David

Abstract

We assessed severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) reverse transcriptase-polymerase chain reaction (RT-PCR) diagnostic sensitivity and cycle threshold (Ct) values relative to symptom onset in symptomatic coronavirus disease-2019 (COVID-19) patients from Bavaria, Germany, of whom a subset was repeatedly tested. Locally weighted scatterplot smoothing method was used to assess the relationship between symptom onset and Ct-values. Kaplan−Meier plots were used to visualise the empirical probability of detecting viral ribonucleic acid (RNA) over time and estimate the time until clearance of viral RNA among the repeatedly tested patients. Among 721 reported COVID-19 cases, the viral RNA was detected in specimens taken between three days before and up to 48 days after symptom onset. The mean Ct-value was 28.6 (95% confidence interval (CI) 28.2–29.0) with the lowest mean Ct-value (26.2) observed two days after symptom onset. Up to 7 days after symptom onset, the diagnostic sensitivity of the RT-PCR among repeatedly sampled patients (n = 208) remained above 90% and decreased to 50% at day 12 (95% CI 10.5–21.5). Our data provide valuable estimates to optimise the timing of sampling of individuals for SARS-CoV-2 detection. A considerable proportion of specimens sampled before symptom onset had Ct-values comparable with Ct-values after symptom onset, suggesting the probability of presymptomatic transmission. [ABSTRACT FROM AUTHOR]

Published
2021
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43. Virological COVID-19 surveillance in Bavaria, Germany suggests no SARS-CoV-2 spread prior to the first German case in January 2020.

Author

Eberle, Ute, Heinzinger, Susanne, Konrad, Regina, Wimmer, Clara, Liebl, Bernhard, Katz, Katharina, Ackermann, Nikolaus, Sing, Andreas, Bavarian SARS-CoV-Public Health Laboratory and Epidemiology Team, Bengs, Katja, Berger, Anja, Böhm, Stefanie, Böhmer, Merle, Dangel, Alexandra, Fingerle, Volker, Hepner, Sabrina, Hobmaier, Bernhard, Hoch, Martin, Lupoli, Gaia, and Margos, Gabriele

Subjects
PUBLIC health surveillance, REVERSE transcriptase polymerase chain reaction, COVID-19, CONFIDENCE intervals, RETROSPECTIVE studies, INFLUENZA, DESCRIPTIVE statistics, POLYMERASE chain reaction, ODDS ratio, COVID-19 testing
Abstract

The Bavarian Influenza Sentinel (BIS) monitors the annual influenza season by combining virological and epidemiological data. The 2019/2020 influenza season overlapped with the beginning COVID-19 pandemic thus allowing to investigate whether there was an unnoticed spread of SARS-CoV-2 among outpatients with acute respiratory infections in the community prior to the first COVID-19 cluster in Bavaria. Therefore, we retrospectively analysed oropharyngeal swabs obtained in BIS between calendar week (CW) 39 in 2019 and CW 14 in 2020 for the presence of SARS-CoV-2 RNA by RT-PCR. 610 of all 1376 BIS swabs-contained sufficient material to test for SARS-CoV-2, among them 260 oropharyngeal swabs which were collected prior to the first notified German COVID-19 case in CW 04/2020. In none of these swabs SARS-CoV-2 RNA was detected suggesting no SARS-CoV-2 spread prior to late January 2020 in Bavaria. [ABSTRACT FROM AUTHOR]

Published
2021
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44. Outbreak of COVID-19 in Germany Resulting from a Single Travel-Associated Primary Case

Author

Böhmer, Merle M., primary, Buchholz, Udo, additional, Corman, Victor M., additional, Hoch, Martin, additional, Katz, Katharina, additional, Marosevic, Durdica V., additional, Böhm, Stefanie, additional, Woudenberg, Tom, additional, Ackermann, Nikolaus, additional, Konrad, Regina, additional, Eberle, Ute, additional, Treis, Bianca, additional, Dangel, Alexandra, additional, Bengs, Katja, additional, Fingerle, Volker, additional, Berger, Anja, additional, Hörmansdorfer, Stefan, additional, Ippisch, Siegfried, additional, Wicklein, Bernd, additional, Grahl, Andreas, additional, Pörtner, Kirsten, additional, Muller, Nadine, additional, Zeitlmann, Nadine, additional, Boender, T. Sonia, additional, Cai, Wei, additional, Reich, Andreas, additional, an der Heiden, Maria, additional, Rexroth, Ute, additional, Hamouda, Osamah, additional, Schneider, Julia, additional, Veith, Talitha, additional, Mühlemann, Barbara, additional, Wölfel, Roman, additional, Antwerpen, Markus, additional, Walter, Mathias, additional, Protzer, Ulrike, additional, Liebl, Bernhard, additional, Haas, Walter, additional, Sing, Andreas, additional, Drosten, Christian, additional, and Zapf, Andreas, additional

Published
2020
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45. Comparison of nine different commercially available molecular assays for detection of SARS-CoV-2 RNA.

Author

Eberle, Ute, Wimmer, Clara, Huber, Ingrid, Neubauer-Juric, Antonie, Valenza, Giuseppe, Ackermann, Nikolaus, Sing, Andreas, for the Bavarian SARS-CoV-2-Public Health Laboratory Team, Baiker, Armin, Bartha-Dima, Bernadett, Bengs, Katja, Berger, Anja, Boll, Kerstin, Carl, Anja, Christian, Jürgen, Dangel, Alexandra, Drdlicek, Juliana, Eisenberger, David, Fingerle, Volker, and Gerdes, Lars

Subjects
*SARS-CoV-2, *COVID-19 pandemic, *RNA, *COVID-19, *COMMUNICABLE diseases
Abstract

To face the COVID-19 pandemic, the need for fast and reliable diagnostic assays for the detection of SARS-CoV-2 is immense. We describe our laboratory experiences evaluating nine commercially available real-time RT-PCR assays. We found that assays differed considerably in performance and validation before routine use is mandatory. [ABSTRACT FROM AUTHOR]

Published
2021
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46. Screening for infectious diseases among newly arrived asylum seekers, Bavaria, Germany, 2015

Author

Ackermann, Nikolaus, primary, Marosevic, Durdica, additional, Hörmansdorfer, Stefan, additional, Eberle, Ute, additional, Rieder, Gabriele, additional, Treis, Bianca, additional, Berger, Anja, additional, Bischoff, Heribert, additional, Bengs, Katja, additional, Konrad, Regina, additional, Hautmann, Wolfgang, additional, Schönberger, Katharina, additional, Belting, Anne, additional, Schlenk, Gisela, additional, Margos, Gabriele, additional, Hoch, Martin, additional, Pürner, Friedrich, additional, Fingerle, Volker, additional, Liebl, Bernhard, additional, and Sing, Andreas, additional

Published
2018
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47. Whole genome sequencing identifies influenza A H3N2 transmission and offers superior resolution to classical typing methods.

Author

Meinel, Dominik M., Heinzinger, Susanne, Eberle, Ute, Ackermann, Nikolaus, Schönberger, Katharina, and Sing, Andreas

Subjects
COMPARATIVE studies, EPIDEMICS, INFLUENZA A virus, H3N2 subtype, GENETIC mutation, POLYMERASE chain reaction, PUBLIC health surveillance, STATISTICAL sampling, SENTINEL health events, SEQUENCE analysis
Abstract

Objectives: Influenza with its annual epidemic waves is a major cause of morbidity and mortality worldwide. However, only little whole genome data are available regarding the molecular epidemiology promoting our understanding of viral spread in human populations.Methods: We implemented a RT-PCR strategy starting from patient material to generate influenza A whole genome sequences for molecular epidemiological surveillance. Samples were obtained within the Bavarian Influenza Sentinel. The complete influenza virus genome was amplified by a one-tube multiplex RT-PCR and sequenced on an Illumina MiSeq.Results: We report whole genomic sequences for 50 influenza A H3N2 viruses, which was the predominating virus in the season 2014/15, directly from patient specimens. The dataset included random samples from Bavaria (Germany) throughout the influenza season and samples from three suspected transmission clusters. We identified the outbreak samples based on sequence identity. Whole genome sequencing (WGS) was superior in resolution compared to analysis of single segments or partial segment analysis. Additionally, we detected manifestation of substantial amounts of viral quasispecies in several patients, carrying mutations varying from the dominant virus in each patient.Conclusion: Our rapid whole genome sequencing approach for influenza A virus shows that WGS can effectively be used to detect and understand outbreaks in large communities. Additionally, the genomic data provide in-depth details about the circulating virus within one season. [ABSTRACT FROM AUTHOR]

Published
2018
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48. Safety of Probiotic Escherichia coli Strain Nissle 1917 Depends on Intestinal Microbiota and Adaptive Immunity of the Host

Author

Gronbach, Kerstin, primary, Eberle, Ute, additional, Müller, Martina, additional, Ölschläger, Tobias A., additional, Dobrindt, Ulrich, additional, Leithäuser, Frank, additional, Niess, Jan Hendrik, additional, Döring, Gerd, additional, Reimann, Jörg, additional, Autenrieth, Ingo B., additional, and Frick, Julia-Stefanie, additional

Published
2010
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49. McDonald's in the classroom

Author

Eberle, Ute

Subjects
Corporate sponsorship -- Analysis, Education -- Finance, Schools -- Advertising
Published
2000

50. Safety of Probiotic Escherichia coliStrain Nissle 1917 Depends on Intestinal Microbiota and Adaptive Immunity of the Host

Author

Gronbach, Kerstin, Eberle, Ute, Müller, Martina, Ölschläger, Tobias A., Dobrindt, Ulrich, Leithäuser, Frank, Niess, Jan Hendrik, Döring, Gerd, Reimann, Jörg, Autenrieth, Ingo B., and Frick, Julia-Stefanie

Abstract

ABSTRACTProbiotics are viable microorganisms that are increasingly used for treatment of a variety of diseases. Occasionally, however, probiotics may have adverse clinical effects, including septicemia. Here we examined the role of the intestinal microbiota and the adaptive immune system in preventing translocation of probiotics (e.g., EscherichiacoliNissle). We challenged C57BL/6J mice raised under germfree conditions (GF-raised C57BL/6J mice) and Rag1−/−mice raised under germfree conditions (GF-raised Rag1−/−mice) and under specific-pathogen-free conditions (SPF-raised Rag1−/−mice) with probiotic E. colistrain Nissle 1917, strain Nissle 1917 mutants, the commensal strain E. colimpk, or Bacteroides vulgatusmpk. Additionally, we reconstituted Rag1−/−mice with CD4+T cells. E. colitranslocation and dissemination and the mortality of mice were assessed. In GF-raised Rag1−/−mice, but not in SPF-raised Rag1−/−mice or GF-raised C57BL/6J mice, oral challenge with E. colistrain Nissle 1917, but not oral challenge with E. colimpk, resulted in translocation and dissemination. The mortality rate was significantly higher for E. colistrain Nissle 1917-challenged GF-raised Rag1−/−mice (100%; P< 0.001) than for E. colistrain Nissle 1917-challenged SPF-raised Rag1−/−mice (0%) and GF-raised C57BL/6J mice (0%). Translocation of and mortality due to strain E. coliNissle 1917 in GF-raised Rag1−/−mice were prevented when mice were reconstituted with T cells prior to strain E. coliNissle 1917 challenge, but not when mice were reconstituted with T cells after E. colistrain Nissle 1917 challenge. Cocolonization experiments revealed that E. colimpk could not prevent translocation of strain E. coliNissle 1917. Moreover, we demonstrated that neither lipopolysaccharide structure nor flagella play a role in E. colistrain Nissle 1917 translocation and dissemination. Our results suggest that if both the microbiota and adaptive immunity are defective, translocation across the intestinal epithelium and dissemination of the probiotic E. colistrain Nissle 1917 may occur and have potentially severe adverse effects. Future work should define the possibly related molecular factors that promote probiotic functions, fitness, and facultative pathogenicity.

Published
2010
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