Additional file 3 of Rapid point-of-care detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Additional file 3: Figure S2. Determination of the sensitivity of the assay. Isolated RNA from SARS-CoV-2 infected Vero cell culture supernatant was quantified using RT-ddPCR and serially diluted to determine the LOD. 1000 copies/µL and 200 copies/µL dilutions were analysed in six replicates, while every lower dilution (1000–0.2 copies/µL) were analysed in 12 replicates. For both target genes ORF8 (A) and N (B) the LOD is 100 copies/µL as the last dilution where all 12 replicates are positive. For non-template control (NTC) PCR grade water substituted SARS-CoV-2 RNA.