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256 results on '"Duprez, Delphine"'

6. Inhibitory SMAD6 interferes with BMP dependent generation of muscle progenitor cells and perturbs proximodistal pattern of murine limb muscles

Author

Asfour, Hasan, primary, Hirsinger, Estelle, additional, Rouco, Raquel, additional, Zarrouki, Faouzi, additional, Hayashi, Shinichiro, additional, Swist, Sandra, additional, Braun, Thomas, additional, Patel, Ketan, additional, Relaix, Frédéric, additional, Andrey, Guillaume, additional, Stricker, Sigmar, additional, Duprez, Delphine, additional, Stantzou, Amalia, additional, and Amthor, Helge, additional

Published
2023
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10. Odd skipped-related 1 identifies a population of embryonic fibro-adipogenic progenitors regulating myogenesis during limb development

Author

Vallecillo-García, Pedro, Orgeur, Mickael, vom Hofe-Schneider, Sophie, Stumm, Jürgen, Kappert, Verena, Ibrahim, Daniel M., Börno, Stefan T., Hayashi, Shinichiro, Relaix, Frédéric, Hildebrandt, Katrin, Sengle, Gerhard, Koch, Manuel, Timmermann, Bernd, Marazzi, Giovanna, Sassoon, David A., Duprez, Delphine, and Stricker, Sigmar

Published
2017
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15. Transcription factor EGR1 directs tendon differentiation and promotes tendon repair

Author

Guerquin, Marie-Justine, Charvet, Benjamin, Nourissat, Geoffroy, Havis, Emmanuelle, Ronsin, Olivier, Bonnin, Marie-Ange, Ruggiu, Mathilde, Olivera-Martinez, Isabel, Robert, Nicolas, Lu, Yinhui, Kadler, Karl E., Baumberger, Tristan, Doursounian, Levon, Berenbaum, Francis, and Duprez, Delphine

Subjects
Physiological aspects, Genetic aspects, Research, Regeneration (Biology) -- Research, Stem cells -- Physiological aspects, Tendons -- Physiological aspects -- Genetic aspects, Transcription factors -- Research
Abstract

Tendon formation and repair rely on specific combinations of transcription factors, growth factors, and mechanical parameters that regulate the production and spatial organization of type I collagen. Here, we investigated [...]

Published
2013
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17. Unexpected contribution of fibroblasts to muscle lineage as a mechanism for limb muscle patterning

Author

CMM Groep Coffer, Hubrecht Institute with UMC, Esteves de Lima, Joana, Blavet, Cédrine, Bonnin, Marie Ange, Hirsinger, Estelle, Comai, Glenda, Yvernogeau, Laurent, Delfini, Marie Claire, Bellenger, Léa, Mella, Sébastien, Nassari, Sonya, Robin, Catherine, Schweitzer, Ronen, Fournier-Thibault, Claire, Jaffredo, Thierry, Tajbakhsh, Shahragim, Relaix, Frédéric, Duprez, Delphine, CMM Groep Coffer, Hubrecht Institute with UMC, Esteves de Lima, Joana, Blavet, Cédrine, Bonnin, Marie Ange, Hirsinger, Estelle, Comai, Glenda, Yvernogeau, Laurent, Delfini, Marie Claire, Bellenger, Léa, Mella, Sébastien, Nassari, Sonya, Robin, Catherine, Schweitzer, Ronen, Fournier-Thibault, Claire, Jaffredo, Thierry, Tajbakhsh, Shahragim, Relaix, Frédéric, and Duprez, Delphine

Published
2021

19. Fgf4 positively regulates scleraxis and tenascin expression in chick limb tendons

Author

Edom-Vovard, Frederique, Schuler, Bernadette, Bonnin, Marie-Ange, Teillet, Marie-Aimee, and Duprez, Delphine

Subjects
Biological sciences
Abstract

In vertebrates, tendons connect muscles to skeletal elements. Surgical experiments in the chick have underlined developmental interactions between tendons and muscles. Initial formation of tendons occurs autonomously with respect to muscle. However, further tendon development requires the presence of muscle. The molecular signals involved in these interactions remain unknown. In the chick limb, Fgf4 transcripts are located at the extremities of muscles, where the future tendons will attach. In this paper, we analyse the putative role of muscle-Fgf4 on tendon development. We have used three general tendon markers, scleraxis, tenascin, and Fgf8 to analyse the regulation of these tendon-associated molecules by Fgf4 under different experimental conditions. In the absence of Fgf4, in muscleless and aneural limbs, the expression of the three tendon-associated molecules, scleraxis, tenascin, and Fgf8, is down-regulated. Exogenous implantation of Fgf4 in normal, aneural, and muscleless limbs induces scleraxis and tenascin expression but not that of Fgf8. These results indicate that Fgf4 expressed in muscle is required for the maintenance of scleraxis and tenascin but not Fgf8 expression in tendons. Key Words: Fgf4; Fgf8; scleraxis; tenascin; tendons; limb bud; chick embryo.

Published
2002

20. Core–Shell Pure Collagen Threads Extruded from Highly Concentrated Solutions Promote Colonization and Differentiation of C3H10T1/2 Cells

Author

Picaut, Lise, primary, Trichet, Léa, additional, Hélary, Christophe, additional, Ducourthial, Guillaume, additional, Bonnin, Marie-Ange, additional, Haye, Bernard, additional, Ronsin, Olivier, additional, Schanne-Klein, Marie-Claire, additional, Duprez, Delphine, additional, Baumberger, Tristan, additional, and Mosser, Gervaise, additional

Published
2021
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21. BMP Signaling Determines the Proximodistal Pattern of Limb Muscles by Spatiotemporal Control of Myogenic Progenitor Cells

Author

Asfour, Hasan, primary, Hirsinger, Estelle, additional, Rouco, Raquel, additional, Zarrouki, Faouzi, additional, Murgai, Arunima, additional, Hayashi, Shinichiro, additional, Swist, Sandra, additional, Braun, Thomas, additional, Patel, Ketan, additional, Relaix, Fred, additional, Andrey, Guillaume, additional, Duprez, Delphine, additional, Stricker, Sigmar, additional, Stantzou, Amalia, additional, and Amthor, Helge, additional

Published
2021
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23. BMP signalling directs a fibroblast-to-myoblast conversion at the connective tissue/muscle interface to pattern limb muscles

Author

de Lima, Joana Esteves, primary, Blavet, Cédrine, additional, Bonnin, Marie-Ange, additional, Hirsinger, Estelle, additional, Comai, Glenda, additional, Yvernogeau, Laurent, additional, Bellenger, Léa, additional, Mella, Sébastien, additional, Nassari, Sonya, additional, Robin, Catherine, additional, Schweitzer, Ronen, additional, Fournier-Thibault, Claire, additional, Tajbakhsh, Shahragim, additional, Relaix, Frédéric, additional, and Duprez, Delphine, additional

Published
2020
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26. Molecular Sciences EGR1 Transcription Factor is a Multifaceted Regulator of Matrix Production in Tendons and Other Connective Tissues

Author

Havis, Emmanuelle, Duprez, Delphine, Laboratoire de Biologie du Développement [Paris] (LBD), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Gestionnaire, Hal Sorbonne Université, and Laboratoire de Biologie du Développement [IBPS] (LBD)

Subjects
collagen, endocrine system, tendon, extracellular matrix, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], connective tissues, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], EGR1, adipose tissue
Abstract

International audience; Although the transcription factor EGR1 is known as NGF1-A, TIS8, Krox24, zif/268, and ZENK, it still has many fewer names than biological functions. A broad range of signals induce Egr1 gene expression via numerous regulatory elements identified in the Egr1 promoter. EGR1 is also the target of multiple post-translational modifications, which modulate EGR1 transcriptional activity. Despite the myriad regulators of Egr1 transcription and translation, and the numerous biological functions identified for EGR1, the literature reveals a recurring theme of EGR1 transcriptional activity in connective tissues, regulating genes related to the extracellular matrix. Egr1 is expressed in different connective tissues, such as tendon (a dense connective tissue), cartilage and bone (supportive connective tissues), and adipose tissue (a loose connective tissue). Egr1 is involved in the development, homeostasis, and healing processes of these tissues, mainly via the regulation of extracellular matrix. In addition, Egr1 is often involved in the abnormal production of extracellular matrix in fibrotic conditions, and Egr1 deletion is seen as a target for therapeutic strategies to fight fibrotic conditions. This generic EGR1 function in matrix regulation has little-explored implications but is potentially important for tendon repair.

Published
2020
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27. Mechanical and molecular parameters that influence the tendon differentiation potential of C3H10T1/2 cells in 2D-and 3D-culture systems

Author

Gaut, Ludovic, Bonnin, Marie-Ange, Blavet, Cédrine, Cacciapuoti, Isabelle, Orpel, Monika, Mericskay, Mathias, Duprez, Delphine, Laboratoire de Biologie du Développement (LBD), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Formation et réparation des muscles et des tendons = Muscle and tendon formation and repair (LBD-E02), Institut National de la Santé et de la Recherche Médicale (INSERM)-Laboratoire de Biologie du Développement (LBD), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Adaptation Biologique et Vieillissement = Biological Adaptation and Ageing (B2A), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie du Développement [Paris] (LBD), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), and Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
musculoskeletal diseases, [SDV]Life Sciences [q-bio], Scleraxis, Cell confluence, Biology, Negative regulator, 03 medical and health sciences, 0302 clinical medicine, Gene expression, medicine, 030304 developmental biology, Murine cell, 0303 health sciences, Chemical treatment, TGFβ2, Mesenchymal stem cell, Tendon cell differentiation, cell cultures, Silicone substrate, Tenomodulin, Tendon, Cell biology, medicine.anatomical_structure, Plastic substrate, TGFb2, Mesenchymal stem cells, 2D-and 3D-cultures, Tendon differentiation, 030217 neurology & neurosurgery
Abstract

One of the main challenges in tendon field relies in the understanding of regulators of the tendon differentiation program. The optimum culture conditions that favor tendon cell differentiation are not identified. Mesenchymal stem cells present the ability to differentiate into multiple lineages in cultures under different cues ranging from chemical treatment to physical constraints. We analyzed the tendon differentiation potential of C3H10T1/2 cells, a murine cell line of mesenchymal stem cells, upon different 2D- and 3D-culture conditions. We observed that C3H10T1/2 cells cultured in 2D conditions on silicone substrate were more prone to tendon differentiation assessed with the expression of the tendon markersScx, Col1a1andTnmdas compared to cells cultured on plastic substrate. 3D fibrin environment was more favorable forScxandCol1a1expression compared to 2D-cultures. We also identified TGFβ2 as a negative regulator ofTnmdexpression in C3H10T1/2 cells in 2D- and 3D-cultures. Altogether, our results provide us with a better understanding of the culture conditions that promote tendon gene expression and identify mechanical and molecular parameters on which we could play to define the optimum culture conditions that favor tenogenic differentiation in mesenchymal stem cells.

Published
2019

28. TMEM8C-mediated fusion is regionalized and regulated by NOTCH signalling during foetal myogenesis.

Author

de Lima, Joana Esteves, Blavet, Cédrine, Bonnin, Marie-Ange, Hirsinger, Estelle, Havis, Emmanuelle, Relaix, Frédéric, and Duprez, Delphine

Subjects
MYOGENESIS, NOTCH effect, CHICKEN embryos, NOTCH genes, MUSCLE growth, MYOBLASTS, SKELETAL muscle
Abstract

The location and regulation of fusion events within skeletal muscles during development remain unknown. Using the fusion marker myomaker (Mymk), named TMEM8C in chicken, as a readout of fusion, we identified a co-segregation of TMEM8C-positive cells and MYOG-positive cells in single-cell RNA-sequencing datasets of limbs from chicken embryos. We found that TMEM8C transcripts, MYOGtranscripts and the fusion-competentMYOG-positive cellswere preferentially regionalized in central regions of foetal muscles. We also identified a similar regionalization for the gene encoding the NOTCH ligand JAG2 along with an absence of NOTCH activity in TMEM8C+ fusion-competent myocytes. NOTCH function in myoblast fusion had not been addressed so far. We analysed the consequences of NOTCH inhibition for TMEM8C expression and myoblast fusion during foetal myogenesis in chicken embryos. NOTCH inhibition increased myoblast fusion and TMEM8C expression and released the transcriptional repressor HEYL from the TMEM8C regulatory regions. These results identify a regionalization of TMEM8C-dependent fusion and a molecular mechanism underlying the fusion-inhibiting effect of NOTCH in foetal myogenesis. The modulation of NOTCH activity in the fusion zone could regulate the flux of fusion events. [ABSTRACT FROM AUTHOR]

Published
2022
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29. Microstructure Manipulation of Polyurethane‐Based Macromolecular Scaffold for Tendon/Ligament Tissue Engineering.

Author

Bahrami, Saeid, Solouk, Atefeh, Duprez, Delphine, and Mirzadeh, Hamid

Subjects
TISSUE engineering, TENDONS, LIGAMENTS, MESENCHYMAL stem cells, MICROSTRUCTURE
Abstract

In spite of improvements in tissue engineering approaches, tendon and ligament (T/L) regeneration is still a clinical challenge. The fabrication of functional scaffolds that possess the structural and biological properties of native T/L tissues is critical to a successful healing. Herein, the effect of scaffold microstructure on mesenchymal stem cells (MSCs) response is evaluated. Electrospun polyurethane (EPU) scaffolds with different alignment degrees of nanofibers are fabricated using five rotating speeds of the collector. Although the constructs display similarity in terms of porosity (85%), the increasing degree of alignment results in a notable reduction of fiber diameter. Accordingly, it also improves the tensile strength from 26.44 ± 0.50 to 35.76 ± 1.50 MPa and tensile modulus from 18.94 ± 1.02 to 31.10 ± 1.11 MPa. Furthermore, despite similarities of cell supporting behavior among all types of scaffolds in terms of cell attachment, spreading, proliferation, and infiltration, the aligned nanofibers noticeably direct cell orientation parallel to their alignment direction, resulting in significant upregulation of tendon‐related markers expression. Taken together, the results highlight that the aligned EPU scaffolds are a promising candidate for T/L regeneration, which deserves to be further investigated with long‐term studies. [ABSTRACT FROM AUTHOR]

Published
2022
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35. Odd skipped-related 1 identifies a population of embryonic fibro-adipogenic progenitors regulating myogenesis during limb development

Author

Martens, Marvin, Vallecillo-García, Pedro, Orgeur, Mickael, vom Hofe-Schneider, Sophie, Stumm, Jürgen, Kappert, Verena, Ibrahim, Daniel, Börno, Stefan, Hayashi, Shinichiro, Relaix, Frédéric, Hildebrandt, Katrin, Sengle, Gerhard, Koch, Manuel, Timmermann, Bernd, Marazzi, Giovanna, Sassoon, David, Duprez, Delphine, Stricker, Sigmar, Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Freie Universität Berlin, Groupe Myologie, Institut de Myologie, Université Pierre et Marie Curie - Paris 6 (UPMC)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Molecular Physiology of Somatic Sensation, Max Planck Institute for Molecular Genetics (MPIMG), Max-Planck-Gesellschaft, Université Pierre et Marie Curie - Paris 6 (UPMC)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie du Développement (LBD), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-IFR10, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Association française contre les myopathies (AFM-Téléthon)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Association française contre les myopathies (AFM-Téléthon)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Association française contre les myopathies (AFM-Téléthon)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Pierre et Marie Curie - Paris 6 (UPMC)

Subjects
[SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017
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36. A dual transcript-discovery approach to improve the delimitation of gene features from RNA-seq data in the chicken model

Author

Orgeur, Mickael, Martens, Marvin, Börno, Stefan, Timmermann, Bernd, Duprez, Delphine, Stricker, Sigmar, Börno, Stefan, Laboratoire de Biologie du Développement (LBD), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Max Planck Institute for Molecular Genetics (MPIMG), Max-Planck-Gesellschaft, Formation et réparation des muscles et des tendons = Muscle and tendon formation and repair (LBD-E02), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institue for Chemistry and Biochemistry, HAL UPMC, Gestionnaire, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
[SDV] Life Sciences [q-bio], Genome-guided transcript discovery, [SDV]Life Sciences [q-bio], Gallus gallus, RNA sequencing, Gene prediction, Chicken genome annotation, Transcriptome reconstruction
Abstract

International audience; The sequence of the chicken genome, like several other draft genome sequences, is presently not fully covered. Gaps, contigs assigned with low confidence and uncharacterized chromosomes result in gene fragmentation and imprecise gene annotation. Transcript abundance estimation from RNA sequencing (RNA-seq) data relies on read quality, library complexity and expression normalization. In addition, the quality of the genome sequence used to map sequencing reads, and the gene annotation that defines gene features, must also be taken into account. A partially covered genome sequence causes the loss of sequencing reads from the mapping step, while an inaccurate definition of gene features induces imprecise read counts from the assignment step. Both steps can significantly bias interpretation of RNA-seq data. Here, we describe a dual transcript-discovery approach combining a genome-guided gene prediction and a de novo transcriptome assembly. This dual approach enabled us to increase the assignment rate of RNA-seq data by nearly 20% as compared to when using only the chicken reference annotation, contributing therefore to a more accurate estimation of transcript abundance. More generally, this strategy could be applied to any organism with partial genome sequence and/ or lacking a manually-curated reference annotation in order to improve the accuracy of gene expression studies.

Published
2017
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41. EGR1 Regulates Transcription Downstream of Mechanical Signals during Tendon Formation and Healing

Author

Gaut, Ludovic, Robert, Nicolas, Delalande, Antony, Bonnin, Marie-Ange, Pichon, Chantal, Duprez, Delphine, Formation et réparation des muscles et des tendons = Muscle and tendon formation and repair (LBD-E02), Laboratoire de Biologie du Développement (LBD), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), HAL-UPMC, Gestionnaire, and Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Critical Care and Emergency Medicine, Transcription, Genetic, Physiology, Gene Expression, lcsh:Medicine, Toxicology, Pathology and Laboratory Medicine, Tendons, Mice, Medicine and Health Sciences, Morphogenesis, Toxins, Homeostasis, lcsh:Science, [SDV.BDD]Life Sciences [q-bio]/Development Biology, Trauma Medicine, Cell Differentiation, musculoskeletal system, Biomechanical Phenomena, Professions, Connective Tissue, Anatomy, Genetic Engineering, Traumatic Injury, Research Article, Biotechnology, Signal Transduction, musculoskeletal diseases, endocrine system, Toxic Agents, Bacterial Toxins, Down-Regulation, Botulinum Toxin, Bone and Bones, Tendon Injuries, Tissue Repair, [SDV.BDD] Life Sciences [q-bio]/Development Biology, Genetics, Animals, Early Growth Response Protein 1, Wound Healing, lcsh:R, Biology and Life Sciences, Engineers, Mice, Inbred C57BL, Biological Tissue, Musculoskeletal Injury, People and Places, Population Groupings, lcsh:Q, Stress, Mechanical, Physiological Processes
Abstract

International audience; BackgroundTendon is a mechanical tissue that transmits forces generated by muscle to bone in order to allow body motion. The molecular pathways that sense mechanical forces during tendon formation, homeostasis and repair are not known. EGR1 is a mechanosensitive transcription factor involved in tendon formation, homeostasis and repair. We hypothesized that EGR1 senses mechanical signals to promote tendon gene expression.Methodology/Principal findingsUsing in vitro and in vivo models, we show that the expression of Egr1 and tendon genes is downregulated in 3D-engineered tendons made of mesenchymal stem cells when tension is released as well as in tendon homeostasis and healing when mechanical signals are reduced. We further demonstrate that EGR1 overexpression prevents tendon gene downregulation in 3D-engineered tendons when tension is released. Lastly, ultrasound and microbubbles mediated EGR1 overexpression prevents the downregulation of tendon gene expression during tendon healing in reduced load conditions.Conclusion/SignificanceThese results show that Egr1 expression is sensitive to mechanical signals in tendon cells. Moreover, EGR1 overexpression prevents the downregulation of tendon gene expression in the absence of mechanical signals in 3D-engineered tendons and tendon healing. These results show that EGR1 induces a transcriptional response downstream of mechanical signals in tendon cells and open new avenues to use EGR1 to promote tendon healing in reduced load conditions.

Published
2016
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50. The Mkx homeoprotein promotes tenogenesis in stem cells and improves tendon repair

Author

Milet, Cécile, Duprez, Delphine, Formation et réparation des muscles et des tendons = Muscle and tendon formation and repair (LBD-E02), Laboratoire de Biologie du Développement (LBD), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), and Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
musculoskeletal diseases, Editorial, musculoskeletal system, [SDV.BDD]Life Sciences [q-bio]/Development Biology
Abstract

International audience; Tendons are essential structures that transmit mechanical forces generated by skeletal muscles to bones to cause body motion. Acute tendon injury and tendinopathy are common pathologies of the musculoskeletal system. However, a poor understanding of tendon biology has impaired the design of efficient treatments for tendon repair following injury and tendinopathy (1). A recent paper by Liu et al. , published in Stem Cells in 2014 (2) highlighted an essential role for the Mohawk (Mkx) homeodomain protein in promoting tenogenesis in mouse stem cells and improving tendon repair in a mouse model for tendon injury.

Published
2015
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