1. P-glycoprotein (ABCB1) is involved in vitamin K efflux
- Author
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Charlotte Halimi, Marielle Margier, Marion Nowicki, Tiffany Antoine, Anne Lespine, Cédric Le May, Emmanuelle Reboul, Le May, Cedric, Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité de recherche de l'institut du thorax (ITX-lab), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Innovations Thérapeutiques et Résistances (InTheRes), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Région PACA in partnership with the Centre Technique de Conservation des Produits Agricoles (CTCPA Avignon), INRAE (French Institute for Agriculture, Food and Environment Research) in partnership with CTCPA Avignon, unité de recherche de l'institut du thorax UMR1087 UMR6291 (ITX), Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
- Subjects
Male ,Vitamin K ,ATP-binding cassette B1 ,Bioavailability ,030309 nutrition & dietetics ,[SDV.MHEP.PHY] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] ,NPC1L1 ,Vitamin k ,Analytical Chemistry ,chemistry.chemical_compound ,scavenger receptor class B type I ,Bile ,PBS ,[SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism ,0303 health sciences ,Chemistry ,SR-BI ,General Medicine ,[SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism ,Postprandial Period ,Recombinant Proteins ,Intestine ,[SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system ,medicine.anatomical_structure ,P glycoprotein abcb1 ,Enterocyte ,Female ,Efflux ,medicine.medical_specialty ,DMEM ,Phylloquinone MDR1 Bioavailability Intestine Enterocyte Bile ABCB1 ,MDR1 ,phosphate-buffered saline ,NPC1 transporter like 1 ,03 medical and health sciences ,[SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system ,Internal medicine ,Phylloquinone ,medicine ,[SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] ,Animals ,Humans ,ATP Binding Cassette Transporter, Subfamily B, Member 1 ,Dulbecco's Modified Eagle's ,030304 developmental biology ,Vitamin D metabolism ,Cholesterol ,CD36 molecule ,Transporter ,[SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology ,Mice, Mutant Strains ,[SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology ,Enterocytes ,Endocrinology ,Caco-2 Cells ,CD36 ,Food Science - Abstract
International audience; ABCB1 (P-glycoprotein/MDR1) is a multidrug efflux transporter that has previously been involved in cholesterol and vitamin D metabolism. Our aim was to explore whether ABCB1 is also involved in vitamin K efflux. Vitamin K apical efflux was significantly decreased in presence of ABCB1 inhibitor in Caco-2 cells (− 20.4%; p < 0.05) and increased in Griptite cells overexpressing ABCB1 (+40.7%; p < 0.05). In vivo, the vitamin K postprandial response was higher in male Abcb1 − /− mice after gavage compared to control animals (+115%; p < 0.05), but was unchanged in female mice. Finally, a vitamin K transintestinal efflux and a biliary vitamin K efflux were observed, but the specific involvement of ABCB1 could not be confirmed in these pathways. Overall, we showed for the first time that ABCB1 is involved in enterocyte vitamin K efflux in both cell and mouse models and regulates vitamin K absorption in mice. Recently, the multidrug membrane transporter ABCB1 (Sharom, 2011) has been identified to contribute to the intestinal transport of vitamin D (Margier et al., 2019
- Published
- 2020